AlfalfaGEDB | MS.gene45735

M. sativa cultivar XinJiangDaYe / MS.gene45735

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45735.t1	KEH30380.1	95.5	200	9	0	1	200	121	320	4.90E-104	387.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45735.t1	Q9XII1	48.5	202	91	4	1	200	117	307	1.5e-40	167.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45735.t1	A0A072UX16	95.5	200	9	0	1	200	121	320	3.5e-104	387.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049149	MS.gene45735	0.806856	6.71E-50	-1.69E-46
MS.gene049195	MS.gene45735	0.820621	6.23E-53	-1.69E-46
MS.gene049297	MS.gene45735	0.805116	1.56E-49	-1.69E-46
MS.gene050330	MS.gene45735	-0.849273	3.59E-60	-1.69E-46
MS.gene050558	MS.gene45735	0.81939	1.19E-52	-1.69E-46
MS.gene050642	MS.gene45735	0.80484	1.78E-49	-1.69E-46
MS.gene050820	MS.gene45735	0.810316	1.22E-50	-1.69E-46
MS.gene051983	MS.gene45735	0.806483	8.05E-50	-1.69E-46
MS.gene052176	MS.gene45735	0.822825	1.93E-53	-1.69E-46
MS.gene052309	MS.gene45735	-0.860899	1.50E-63	-1.69E-46
MS.gene052358	MS.gene45735	-0.849938	2.34E-60	-1.69E-46
MS.gene052361	MS.gene45735	-0.843316	1.51E-58	-1.69E-46
MS.gene053054	MS.gene45735	-0.817109	3.91E-52	-1.69E-46
MS.gene053836	MS.gene45735	0.817952	2.53E-52	-1.69E-46
MS.gene054983	MS.gene45735	0.822366	2.46E-53	-1.69E-46
MS.gene057121	MS.gene45735	0.800505	1.40E-48	-1.69E-46
MS.gene057127	MS.gene45735	-0.813853	2.07E-51	-1.69E-46
MS.gene061040	MS.gene45735	-0.865585	5.31E-65	-1.69E-46
MS.gene061114	MS.gene45735	-0.844474	7.39E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45735.t1	MTR_4g068880	95.500	200	9	1	200	121	320	2.45e-143	402
MS.gene45735.t1	MTR_4g068880	95.000	200	10	1	200	121	320	1.08e-142	400
MS.gene45735.t1	MTR_0026s0200	95.500	200	9	1	200	579	778	1.92e-136	400

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45735.t1	AT2G16070	48.039	204	89	6	1	200	34	224	3.14e-51	165
MS.gene45735.t1	AT2G16070	48.039	204	89	6	1	200	34	224	3.14e-51	165
MS.gene45735.t1	AT2G16070	48.039	204	89	6	1	200	117	307	7.10e-51	166

Find 49 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACGAACCTTCTGCAAATT+AGG	0.192387	4.4:-38611451	None:intergenic
GAGATCTACCACTGAAAATA+AGG	0.238835	4.4:+38611516	MS.gene45735:CDS
CCACATCCATAGTTTATATC+AGG	0.316941	4.4:-38611646	None:intergenic
GTTGCAGCAACAGCTGAAAA+TGG	0.342115	4.4:-38611622	None:intergenic
GGAGTATAAAGGAAAAGATT+TGG	0.355923	4.4:+38611090	MS.gene45735:CDS
GTGTCCACCTGGTAGAGTTT+TGG	0.371016	4.4:+38611549	MS.gene45735:CDS
GCAACACCTGATATAAACTA+TGG	0.384435	4.4:+38611640	MS.gene45735:CDS
ATATTGAGTATGTCAGGATT+TGG	0.388003	4.4:+38611427	MS.gene45735:CDS
AATAAGCTCAAGGAGTATAA+AGG	0.395809	4.4:+38611079	MS.gene45735:CDS
TATGGAGGGCGGGTTGGATA+AGG	0.395815	4.4:-38611181	None:intergenic
GGTTGGCTCCACTGTTTCAC+TGG	0.400114	4.4:-38611140	None:intergenic
CACTGTTTCACTGGCAAAAG+TGG	0.402405	4.4:-38611131	None:intergenic
TTGTCAAGAGACATGGAATA+TGG	0.435035	4.4:-38611199	None:intergenic
AGCAAAATCATGTTGAAGAC+GGG	0.457681	4.4:+38611323	MS.gene45735:CDS
AAAGCAGTGCTCACAGTTGT+TGG	0.459690	4.4:+38611394	MS.gene45735:CDS
CACAGACTAGAAAATGAGAA+TGG	0.463269	4.4:+38611493	MS.gene45735:CDS
AGGAGGAAGTAGTAGATCAT+TGG	0.467708	4.4:-38611161	None:intergenic
ATGGAATATGGAGGGCGGGT+TGG	0.479831	4.4:-38611187	None:intergenic
ATCCCTTCTGTCAATAAGTC+CGG	0.488206	4.4:+38611238	MS.gene45735:CDS
GTCAATAAGTCCGGTCGCAA+TGG	0.494855	4.4:+38611247	MS.gene45735:CDS
GTATCAATATTGAGTATGTC+AGG	0.496740	4.4:+38611421	MS.gene45735:CDS
GGAGGGCGGGTTGGATAAGG+AGG	0.511572	4.4:-38611178	None:intergenic
CACACTTGATAATGTGATTG+AGG	0.511821	4.4:+38611276	MS.gene45735:CDS
CTAATTTGCAGAAGGTTCGT+TGG	0.524180	4.4:+38611452	MS.gene45735:CDS
ACAGACTAGAAAATGAGAAT+GGG	0.537258	4.4:+38611494	MS.gene45735:CDS
AGATCTACCACTGAAAATAA+GGG	0.549200	4.4:+38611517	MS.gene45735:CDS
CGACCGGACTTATTGACAGA+AGG	0.550670	4.4:-38611241	None:intergenic
CTCGATGTCTCGTGAAAGAG+AGG	0.553753	4.4:+38611590	MS.gene45735:CDS
ACCACTGAAAATAAGGGTGA+TGG	0.554032	4.4:+38611523	MS.gene45735:CDS
AGACATGGAATATGGAGGGC+GGG	0.557062	4.4:-38611191	None:intergenic
GGTGATGGACAGTGTCCACC+TGG	0.562228	4.4:+38611538	MS.gene45735:CDS
TCCAGCACCAAAACTCTACC+AGG	0.564759	4.4:-38611556	None:intergenic
ATTTGGTCCTAATTTGCAGA+AGG	0.566190	4.4:+38611444	MS.gene45735:CDS
GAGCAAAATCATGTTGAAGA+CGG	0.571685	4.4:+38611322	MS.gene45735:CDS
CCTGATATAAACTATGGATG+TGG	0.573675	4.4:+38611646	MS.gene45735:CDS
TGTGATTAATCCATTGCGAC+CGG	0.579636	4.4:-38611257	None:intergenic
AATGCTAATTAATAAGCTCA+AGG	0.585821	4.4:+38611069	None:intergenic
TCAAGAGACATGGAATATGG+AGG	0.598159	4.4:-38611196	None:intergenic
GACCGGACTTATTGACAGAA+GGG	0.606790	4.4:-38611240	None:intergenic
ATATTGCTTGTCAAGAGACA+TGG	0.613371	4.4:-38611206	None:intergenic
CAAGAGACATGGAATATGGA+GGG	0.618949	4.4:-38611195	None:intergenic
GGAAGTAGTAGATCATTGGT+TGG	0.626172	4.4:-38611157	None:intergenic
TTCGTTGGAATGTACAACGC+AGG	0.626741	4.4:+38611467	MS.gene45735:CDS
TTTGGTGCTGGAAAATGGGG+AGG	0.629531	4.4:+38611567	MS.gene45735:CDS
GAGACATGGAATATGGAGGG+CGG	0.660233	4.4:-38611192	None:intergenic
GCAAAATCATGTTGAAGACG+GGG	0.661988	4.4:+38611324	MS.gene45735:CDS
AGTGCTCACAGTTGTTGGTG+TGG	0.679889	4.4:+38611399	MS.gene45735:CDS
AGCACCAAAACTCTACCAGG+TGG	0.702063	4.4:-38611553	None:intergenic
TCGATGTCTCGTGAAAGAGA+GGG	0.703822	4.4:+38611591	MS.gene45735:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGATTTTGCTCTTTTTCATT+TGG	-	chr4.4:38611313-38611332	None:intergenic	25.0%
	ACAGACTAGAAAATGAGAAT+GGG	+	chr4.4:38611494-38611513	MS.gene45735:CDS	30.0%
	AGATCTACCACTGAAAATAA+GGG	+	chr4.4:38611517-38611536	MS.gene45735:CDS	30.0%
	ATATTGAGTATGTCAGGATT+TGG	+	chr4.4:38611427-38611446	MS.gene45735:CDS	30.0%
	GGAGTATAAAGGAAAAGATT+TGG	+	chr4.4:38611090-38611109	MS.gene45735:CDS	30.0%
	GTATCAATATTGAGTATGTC+AGG	+	chr4.4:38611421-38611440	MS.gene45735:CDS	30.0%
!	AATAAGCTCAAGGAGTATAA+AGG	+	chr4.4:38611079-38611098	MS.gene45735:CDS	30.0%
	AGCAAAATCATGTTGAAGAC+GGG	+	chr4.4:38611323-38611342	MS.gene45735:CDS	35.0%
	ATATTGCTTGTCAAGAGACA+TGG	-	chr4.4:38611209-38611228	None:intergenic	35.0%
	CACAGACTAGAAAATGAGAA+TGG	+	chr4.4:38611493-38611512	MS.gene45735:CDS	35.0%
	CCACATCCATAGTTTATATC+AGG	-	chr4.4:38611649-38611668	None:intergenic	35.0%
	CCTGATATAAACTATGGATG+TGG	+	chr4.4:38611646-38611665	MS.gene45735:CDS	35.0%
	GAGATCTACCACTGAAAATA+AGG	+	chr4.4:38611516-38611535	MS.gene45735:CDS	35.0%
	GAGCAAAATCATGTTGAAGA+CGG	+	chr4.4:38611322-38611341	MS.gene45735:CDS	35.0%
	GCAACACCTGATATAAACTA+TGG	+	chr4.4:38611640-38611659	MS.gene45735:CDS	35.0%
	GGGGCTAAAAATTCAAGAAA+AGG	+	chr4.4:38611343-38611362	MS.gene45735:CDS	35.0%
	TTGTCAAGAGACATGGAATA+TGG	-	chr4.4:38611202-38611221	None:intergenic	35.0%
!	ATTTGGTCCTAATTTGCAGA+AGG	+	chr4.4:38611444-38611463	MS.gene45735:CDS	35.0%
!!	CACACTTGATAATGTGATTG+AGG	+	chr4.4:38611276-38611295	MS.gene45735:CDS	35.0%
	ACCACTGAAAATAAGGGTGA+TGG	+	chr4.4:38611523-38611542	MS.gene45735:CDS	40.0%
	AGGAGGAAGTAGTAGATCAT+TGG	-	chr4.4:38611164-38611183	None:intergenic	40.0%
	ATCCCTTCTGTCAATAAGTC+CGG	+	chr4.4:38611238-38611257	MS.gene45735:CDS	40.0%
	CAACGAACCTTCTGCAAATT+AGG	-	chr4.4:38611454-38611473	None:intergenic	40.0%
	CAAGAGACATGGAATATGGA+GGG	-	chr4.4:38611198-38611217	None:intergenic	40.0%
	GCAAAATCATGTTGAAGACG+GGG	+	chr4.4:38611324-38611343	MS.gene45735:CDS	40.0%
	GGAAGTAGTAGATCATTGGT+TGG	-	chr4.4:38611160-38611179	None:intergenic	40.0%
	TCAAGAGACATGGAATATGG+AGG	-	chr4.4:38611199-38611218	None:intergenic	40.0%
	TGTGATTAATCCATTGCGAC+CGG	-	chr4.4:38611260-38611279	None:intergenic	40.0%
!	CTAATTTGCAGAAGGTTCGT+TGG	+	chr4.4:38611452-38611471	MS.gene45735:CDS	40.0%
!!!	AGAGTTTTGGTGCTGGAAAA+TGG	+	chr4.4:38611562-38611581	MS.gene45735:CDS	40.0%
!!!	AGTTTTGGTGCTGGAAAATG+GGG	+	chr4.4:38611564-38611583	MS.gene45735:CDS	40.0%
!!!	GAGTTTTGGTGCTGGAAAAT+GGG	+	chr4.4:38611563-38611582	MS.gene45735:CDS	40.0%
!!!	TCCATCACCCTTATTTTCAG+TGG	-	chr4.4:38611527-38611546	None:intergenic	40.0%
	AAAGCAGTGCTCACAGTTGT+TGG	+	chr4.4:38611394-38611413	MS.gene45735:CDS	45.0%
	CACTGTTTCACTGGCAAAAG+TGG	-	chr4.4:38611134-38611153	None:intergenic	45.0%
	GACCGGACTTATTGACAGAA+GGG	-	chr4.4:38611243-38611262	None:intergenic	45.0%
	GTTGCAGCAACAGCTGAAAA+TGG	-	chr4.4:38611625-38611644	None:intergenic	45.0%
	TCGATGTCTCGTGAAAGAGA+GGG	+	chr4.4:38611591-38611610	MS.gene45735:CDS	45.0%
	TTCGTTGGAATGTACAACGC+AGG	+	chr4.4:38611467-38611486	MS.gene45735:CDS	45.0%
!	CACTTTTGCCAGTGAAACAG+TGG	+	chr4.4:38611132-38611151	MS.gene45735:CDS	45.0%
	AGACATGGAATATGGAGGGC+GGG	-	chr4.4:38611194-38611213	None:intergenic	50.0%
	AGCACCAAAACTCTACCAGG+TGG	-	chr4.4:38611556-38611575	None:intergenic	50.0%
	AGTGCTCACAGTTGTTGGTG+TGG	+	chr4.4:38611399-38611418	MS.gene45735:CDS	50.0%
	CGACCGGACTTATTGACAGA+AGG	-	chr4.4:38611244-38611263	None:intergenic	50.0%
	CTCGATGTCTCGTGAAAGAG+AGG	+	chr4.4:38611590-38611609	MS.gene45735:CDS	50.0%
	GAGACATGGAATATGGAGGG+CGG	-	chr4.4:38611195-38611214	None:intergenic	50.0%
	GTCAATAAGTCCGGTCGCAA+TGG	+	chr4.4:38611247-38611266	MS.gene45735:CDS	50.0%
	TCCAGCACCAAAACTCTACC+AGG	-	chr4.4:38611559-38611578	None:intergenic	50.0%
!	GTGTCCACCTGGTAGAGTTT+TGG	+	chr4.4:38611549-38611568	MS.gene45735:CDS	50.0%
!!	ACCTGGTAGAGTTTTGGTGC+TGG	+	chr4.4:38611555-38611574	MS.gene45735:CDS	50.0%
!!	TTTGGTGCTGGAAAATGGGG+AGG	+	chr4.4:38611567-38611586	MS.gene45735:CDS	50.0%
	ATGGAATATGGAGGGCGGGT+TGG	-	chr4.4:38611190-38611209	None:intergenic	55.0%
!	GGTTGGCTCCACTGTTTCAC+TGG	-	chr4.4:38611143-38611162	None:intergenic	55.0%
!!	TATGGAGGGCGGGTTGGATA+AGG	-	chr4.4:38611184-38611203	None:intergenic	55.0%
!!	GGTGATGGACAGTGTCCACC+TGG	+	chr4.4:38611538-38611557	MS.gene45735:CDS	60.0%
!!	GGAGGGCGGGTTGGATAAGG+AGG	-	chr4.4:38611181-38611200	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	38611070	38611672	38611070	ID=MS.gene45735
chr4.4	mRNA	38611070	38611672	38611070	ID=MS.gene45735.t1;Parent=MS.gene45735
chr4.4	exon	38611070	38611672	38611070	ID=MS.gene45735.t1.exon1;Parent=MS.gene45735.t1
chr4.4	CDS	38611070	38611672	38611070	ID=cds.MS.gene45735.t1;Parent=MS.gene45735.t1

Gene Sequence

>MS.gene45735

ATGCTAATTAATAAGCTCAAGGAGTATAAAGGAAAAGATTTGGAAGTGATACATGAAGCTTCCACTTTTGCCAGTGAAACAGTGGAGCCAACCAATGATCTACTACTTCCTCCTTATCCAACCCGCCCTCCATATTCCATGTCTCTTGACAAGCAATATCTGTCACAGATCCCTTCTGTCAATAAGTCCGGTCGCAATGGATTAATCACACTTGATAATGTGATTGAGGCTAAAAAGAATCCAAATGAAAAAGAGCAAAATCATGTTGAAGACGGGGCTAAAAATTCAAGAAAAGGTTTACGATTTTTTATAACTTCTGCAGCAAAAGCAGTGCTCACAGTTGTTGGTGTGGTATCAATATTGAGTATGTCAGGATTTGGTCCTAATTTGCAGAAGGTTCGTTGGAATGTACAACGCAGGCATCACAGACTAGAAAATGAGAATGGGAGATCTACCACTGAAAATAAGGGTGATGGACAGTGTCCACCTGGTAGAGTTTTGGTGCTGGAAAATGGGGAGGCTCGATGTCTCGTGAAAGAGAGGGTTGAAATTCCATTTTCAGCTGTTGCTGCAACACCTGATATAAACTATGGATGTGGTTAA

Protein sequence

>MS.gene45735.t1

MLINKLKEYKGKDLEVIHEASTFASETVEPTNDLLLPPYPTRPPYSMSLDKQYLSQIPSVNKSGRNGLITLDNVIEAKKNPNEKEQNHVEDGAKNSRKGLRFFITSAAKAVLTVVGVVSILSMSGFGPNLQKVRWNVQRRHHRLENENGRSTTENKGDGQCPPGRVLVLENGEARCLVKERVEIPFSAVAATPDINYGCG