AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049195


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049195.t1 MTR_3g098170 96.026 151 6 0 2 152 10 160 1.39e-104 296
MS.gene049195.t1 MTR_3g098160 76.923 156 31 1 2 152 10 165 7.03e-86 248
MS.gene049195.t1 MTR_3g098180 76.623 154 33 1 2 152 5 158 3.66e-84 244
MS.gene049195.t1 MTR_3g098130 72.727 154 39 1 2 152 10 163 7.30e-79 231
MS.gene049195.t1 MTR_3g098130 72.727 154 39 1 2 152 48 201 1.94e-78 231
MS.gene049195.t1 MTR_3g098140 74.126 143 32 1 15 152 14 156 2.17e-74 219
MS.gene049195.t1 MTR_3g098100 68.590 156 44 3 1 152 1 155 2.68e-70 209
MS.gene049195.t1 MTR_3g098170 93.458 107 7 0 2 108 10 116 1.15e-69 206
MS.gene049195.t1 MTR_3g098120 66.225 151 44 2 3 152 52 196 7.43e-66 199
MS.gene049195.t1 MTR_3g098160 78.846 104 22 0 2 105 10 113 1.67e-57 176
MS.gene049195.t1 MTR_5g069640 32.278 158 96 3 4 152 6 161 1.34e-21 85.5
MS.gene049195.t1 MTR_8g032450 30.469 128 87 2 26 152 61 187 5.67e-11 58.2
MS.gene049195.t1 MTR_8g433000 30.469 128 87 2 26 152 61 187 5.67e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049195.t1 AT1G04290 60.667 150 54 2 1 146 1 149 1.75e-57 176
MS.gene049195.t1 AT1G04290 54.128 109 45 2 1 105 1 108 5.44e-32 111
MS.gene049195.t1 AT2G29590 34.161 161 91 4 3 152 2 158 4.07e-21 84.3

Find 35 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAATTTCCTCCCAAGTTTC+TGG 0.072394 3.3:-80785172 MS.gene049195:CDS
ACTGACAAAGGGAAGATATT+TGG 0.225921 3.3:+80783877 None:intergenic
AGAATCAGTGAAGAGGTATT+TGG 0.264125 3.3:-80785223 MS.gene049195:CDS
TTAATTAACACGCAGAATTC+AGG 0.290014 3.3:-80784991 MS.gene049195:intron
GCTGGATTTCCGATGGATAC+TGG 0.400055 3.3:-80784901 MS.gene049195:CDS
CATCGTGCGTGGCCTACGAT+TGG 0.426469 3.3:-80785142 MS.gene049195:CDS
GATGAAAGGTTCCAGAAACT+TGG 0.442003 3.3:+80785161 None:intergenic
GCAATTGCAATTCCTGCCGC+TGG 0.476508 3.3:-80784919 MS.gene049195:CDS
CGTAGGCCACGCACGATGAA+AGG 0.480275 3.3:+80785147 None:intergenic
CATTGTTACTCTGGTGGATG+TGG 0.494972 3.3:-80784947 MS.gene049195:CDS
CGTTGGTGCCATTGTTACTC+TGG 0.516580 3.3:-80784956 MS.gene049195:CDS
ATTGAGAAAACGACACGGCC+AGG 0.517820 3.3:+80785108 None:intergenic
TCCTGCCGCTGGATTTCCGA+TGG 0.525258 3.3:-80784908 MS.gene049195:CDS
CGATTGGATCTCATTGAGCC+TGG 0.533177 3.3:-80785126 MS.gene049195:CDS
CTGGAACCTTTCATCGTGCG+TGG 0.538244 3.3:-80785153 MS.gene049195:CDS
TCCATCGGAAATCCAGCGGC+AGG 0.540610 3.3:+80784907 None:intergenic
CAAGGTTCTACGAGTAGGAA+AGG 0.557518 3.3:-80783969 MS.gene049195:CDS
AGCATGAGACATTGATCTCG+AGG 0.588082 3.3:+80784872 None:intergenic
ATGAAAGGTTCCAGAAACTT+GGG 0.589410 3.3:+80785162 None:intergenic
GGAGATTGAGATTGATAACA+AGG 0.592158 3.3:-80783987 MS.gene049195:CDS
GTTACTCTGGTGGATGTGGT+AGG 0.592542 3.3:-80784943 MS.gene049195:CDS
TGGTGCCATTGTTACTCTGG+TGG 0.609784 3.3:-80784953 MS.gene049195:CDS
TGGATTTAGAATCAGTGAAG+AGG 0.614768 3.3:-80785230 MS.gene049195:CDS
CACATCCACCAGAGTAACAA+TGG 0.615960 3.3:+80784948 None:intergenic
GATAACAAGGTTCTACGAGT+AGG 0.624009 3.3:-80783974 MS.gene049195:CDS
GCATGAGACATTGATCTCGA+GGG 0.635278 3.3:+80784873 None:intergenic
AGGGAAACTCCAGTATCCAT+CGG 0.635502 3.3:+80784892 None:intergenic
GCTCAATGAGATCCAATCGT+AGG 0.645816 3.3:+80785130 None:intergenic
AAAGGTTCCAGAAACTTGGG+AGG 0.690498 3.3:+80785165 None:intergenic
AGTATCCATCGGAAATCCAG+CGG 0.693311 3.3:+80784903 None:intergenic
AGAGTACGAACGAGTAAACG+TGG 0.694717 3.3:+80785075 None:intergenic
GGAGAAAAGAGAAACATCAA+CGG 0.699958 3.3:-80785202 MS.gene049195:CDS
TCAGGTAAGCACTTGCACGT+TGG 0.701973 3.3:-80784973 MS.gene049195:CDS
TGTTCATTGAGAAAACGACA+CGG 0.782322 3.3:+80785103 None:intergenic
GTACGAACGAGTAAACGTGG+AGG 0.803457 3.3:+80785078 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAAAATTAGTGTTTA+AGG + chr3.3:80784583-80784602 None:intergenic 15.0%
!! AAAGAAAAATTAGTGTTTAA+GGG + chr3.3:80784582-80784601 None:intergenic 15.0%
!! ATATTATTTAGAAACGAAAA+AGG + chr3.3:80784509-80784528 None:intergenic 15.0%
!! CAAATTTAATACAAAAAACA+AGG + chr3.3:80785024-80785043 None:intergenic 15.0%
!! TATTATTTAGAAACGAAAAA+GGG + chr3.3:80784508-80784527 None:intergenic 15.0%
!!! AATGAAAAACCAATTTTAAT+TGG + chr3.3:80784345-80784364 None:intergenic 15.0%
!!! ATGAAAAACCAATTTTAATT+GGG + chr3.3:80784344-80784363 None:intergenic 15.0%
!! ACAGATGTAAAAAAATTGAA+AGG + chr3.3:80784962-80784981 None:intergenic 20.0%
!! ATAATTGTCCCAATTAAAAT+TGG - chr3.3:80784333-80784352 MS.gene049195:intron 20.0%
!! ATTTAGTCTAGACTAATAAA+GGG - chr3.3:80784693-80784712 MS.gene049195:intron 20.0%
!! CGTTTCTAAATAATATTCAT+TGG - chr3.3:80784513-80784532 MS.gene049195:intron 20.0%
!! GCGTGTTAATTAAATAATTA+AGG + chr3.3:80784101-80784120 None:intergenic 20.0%
!! TATTTAGTCTAGACTAATAA+AGG - chr3.3:80784692-80784711 MS.gene049195:intron 20.0%
!!! ATTAGTCTAGACTAAATAAA+TGG + chr3.3:80784690-80784709 None:intergenic 20.0%
!!! TTTTTTTGTTTAATTCCCTA+TGG - chr3.3:80785072-80785091 MS.gene049195:intron 20.0%
! AAGTTTCAATGAATGACTAA+TGG + chr3.3:80784843-80784862 None:intergenic 25.0%
! ATCATAAAAAAGAGTTTGTC+TGG + chr3.3:80784753-80784772 None:intergenic 25.0%
! ATGATTAACGATAATACAAG+AGG + chr3.3:80784297-80784316 None:intergenic 25.0%
! GATATCAAATTGATCATTAG+AGG - chr3.3:80784982-80785001 MS.gene049195:intron 25.0%
! TCATAAAAAAGAGTTTGTCT+GGG + chr3.3:80784752-80784771 None:intergenic 25.0%
! TGATTAACGATAATACAAGA+GGG + chr3.3:80784296-80784315 None:intergenic 25.0%
!!! AAATAAATGGTCGTTTTCTT+CGG + chr3.3:80784677-80784696 None:intergenic 25.0%
AGAGTTCAGAAAGAAAAAGA+CGG - chr3.3:80785172-80785191 MS.gene049195:CDS 30.0%
GAATTAGCATATGTGCTTAA+TGG + chr3.3:80784394-80784413 None:intergenic 30.0%
GATTAACGATAATACAAGAG+GGG + chr3.3:80784295-80784314 None:intergenic 30.0%
GCTAATTGAATATGAGAATG+AGG - chr3.3:80784786-80784805 MS.gene049195:intron 30.0%
GCTATGAAACAAACTAACAT+TGG + chr3.3:80784268-80784287 None:intergenic 30.0%
TTAATTAACACGCAGAATTC+AGG - chr3.3:80784108-80784127 MS.gene049195:intron 30.0%
TTCTCATATTCAATTAGCAG+TGG + chr3.3:80784784-80784803 None:intergenic 30.0%
! ATACATTACTGTTAGCTTAG+TGG + chr3.3:80784898-80784917 None:intergenic 30.0%
! TACATTACTGTTAGCTTAGT+GGG + chr3.3:80784897-80784916 None:intergenic 30.0%
!! AATAAATGGTCGTTTTCTTC+GGG + chr3.3:80784676-80784695 None:intergenic 30.0%
!! ATAATATTCATTGGCTTGTC+AGG - chr3.3:80784522-80784541 MS.gene049195:intron 30.0%
!! TAATATTCATTGGCTTGTCA+GGG - chr3.3:80784523-80784542 MS.gene049195:intron 30.0%
AAGCAAGAAGAAGAAGATTG+AGG + chr3.3:80784050-80784069 None:intergenic 35.0%
AAGTGAAGCTATACATAGAG+AGG - chr3.3:80784423-80784442 MS.gene049195:intron 35.0%
ACTGACAAAGGGAAGATATT+TGG + chr3.3:80785225-80785244 None:intergenic 35.0%
ATGAAAGGTTCCAGAAACTT+GGG + chr3.3:80783940-80783959 None:intergenic 35.0%
ATTCCCTATGGCTTATGTAA+TGG - chr3.3:80785084-80785103 MS.gene049195:intron 35.0%
CTACCATTACATAAGCCATA+GGG + chr3.3:80785090-80785109 None:intergenic 35.0%
GAGTTCAGAAAGAAAAAGAC+GGG - chr3.3:80785173-80785192 MS.gene049195:CDS 35.0%
GGAGAAAAGAGAAACATCAA+CGG - chr3.3:80783897-80783916 MS.gene049195:CDS 35.0%
TGTTCATTGAGAAAACGACA+CGG + chr3.3:80783999-80784018 None:intergenic 35.0%
! ACATTACTGTTAGCTTAGTG+GGG + chr3.3:80784896-80784915 None:intergenic 35.0%
! GGAGATTGAGATTGATAACA+AGG - chr3.3:80785112-80785131 MS.gene049195:CDS 35.0%
! TGGATTTAGAATCAGTGAAG+AGG - chr3.3:80783869-80783888 MS.gene049195:CDS 35.0%
!! AGAATCAGTGAAGAGGTATT+TGG - chr3.3:80783876-80783895 MS.gene049195:CDS 35.0%
!! CTTCTTCTTGCTTTTGATGA+TGG - chr3.3:80784057-80784076 MS.gene049195:intron 35.0%
ATGGCTTATGTAATGGTAGG+AGG - chr3.3:80785091-80785110 MS.gene049195:CDS 40.0%
CCTACCATTACATAAGCCAT+AGG + chr3.3:80785091-80785110 None:intergenic 40.0%
CCTATGGCTTATGTAATGGT+AGG - chr3.3:80785088-80785107 MS.gene049195:CDS 40.0%
GATAACAAGGTTCTACGAGT+AGG - chr3.3:80785125-80785144 MS.gene049195:CDS 40.0%
GATGAAAGGTTCCAGAAACT+TGG + chr3.3:80783941-80783960 None:intergenic 40.0%
AAAGGTTCCAGAAACTTGGG+AGG + chr3.3:80783937-80783956 None:intergenic 45.0%
AGAGTACGAACGAGTAAACG+TGG + chr3.3:80784027-80784046 None:intergenic 45.0%
AGCATGAGACATTGATCTCG+AGG + chr3.3:80784230-80784249 None:intergenic 45.0%
AGGGAAACTCCAGTATCCAT+CGG + chr3.3:80784210-80784229 None:intergenic 45.0%
AGTATCCATCGGAAATCCAG+CGG + chr3.3:80784199-80784218 None:intergenic 45.0%
CAAGGTTCTACGAGTAGGAA+AGG - chr3.3:80785130-80785149 MS.gene049195:CDS 45.0%
CACATCCACCAGAGTAACAA+TGG + chr3.3:80784154-80784173 None:intergenic 45.0%
CGAATTTCCTCCCAAGTTTC+TGG - chr3.3:80783927-80783946 MS.gene049195:CDS 45.0%
GACGCCCTTGAGCAAAAATT+TGG + chr3.3:80785199-80785218 None:intergenic 45.0%
GCATGAGACATTGATCTCGA+GGG + chr3.3:80784229-80784248 None:intergenic 45.0%
GCTCAATGAGATCCAATCGT+AGG + chr3.3:80783972-80783991 None:intergenic 45.0%
! ACGGGCCAAATTTTTGCTCA+AGG - chr3.3:80785191-80785210 MS.gene049195:CDS 45.0%
! CATTGTTACTCTGGTGGATG+TGG - chr3.3:80784152-80784171 MS.gene049195:intron 45.0%
! CGGGCCAAATTTTTGCTCAA+GGG - chr3.3:80785192-80785211 MS.gene049195:CDS 45.0%
ATTGAGAAAACGACACGGCC+AGG + chr3.3:80783994-80784013 None:intergenic 50.0%
CGATTGGATCTCATTGAGCC+TGG - chr3.3:80783973-80783992 MS.gene049195:CDS 50.0%
GCTGGATTTCCGATGGATAC+TGG - chr3.3:80784198-80784217 MS.gene049195:intron 50.0%
GTACGAACGAGTAAACGTGG+AGG + chr3.3:80784024-80784043 None:intergenic 50.0%
TCAGGTAAGCACTTGCACGT+TGG - chr3.3:80784126-80784145 MS.gene049195:intron 50.0%
! GTTACTCTGGTGGATGTGGT+AGG - chr3.3:80784156-80784175 MS.gene049195:intron 50.0%
!! CATTGGCTTGTCAGGGTGAT+TGG - chr3.3:80784530-80784549 MS.gene049195:intron 50.0%
!! CGTTGGTGCCATTGTTACTC+TGG - chr3.3:80784143-80784162 MS.gene049195:intron 50.0%
!! TGGTGCCATTGTTACTCTGG+TGG - chr3.3:80784146-80784165 MS.gene049195:intron 50.0%
CTGGAACCTTTCATCGTGCG+TGG - chr3.3:80783946-80783965 MS.gene049195:CDS 55.0%
GCAATTGCAATTCCTGCCGC+TGG - chr3.3:80784180-80784199 MS.gene049195:intron 55.0%
CATCGTGCGTGGCCTACGAT+TGG - chr3.3:80783957-80783976 MS.gene049195:CDS 60.0%
CGTAGGCCACGCACGATGAA+AGG + chr3.3:80783955-80783974 None:intergenic 60.0%
TCCATCGGAAATCCAGCGGC+AGG + chr3.3:80784195-80784214 None:intergenic 60.0%
TCCTGCCGCTGGATTTCCGA+TGG - chr3.3:80784191-80784210 MS.gene049195:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 80783868 80785253 80783868 ID=MS.gene049195
chr3.3 mRNA 80783868 80785253 80783868 ID=MS.gene049195.t1;Parent=MS.gene049195
chr3.3 exon 80785086 80785253 80785086 ID=MS.gene049195.t1.exon1;Parent=MS.gene049195.t1
chr3.3 CDS 80785086 80785253 80785086 ID=cds.MS.gene049195.t1;Parent=MS.gene049195.t1
chr3.3 exon 80784852 80784998 80784852 ID=MS.gene049195.t1.exon2;Parent=MS.gene049195.t1
chr3.3 CDS 80784852 80784998 80784852 ID=cds.MS.gene049195.t1;Parent=MS.gene049195.t1
chr3.3 exon 80783868 80784011 80783868 ID=MS.gene049195.t1.exon3;Parent=MS.gene049195.t1
chr3.3 CDS 80783868 80784011 80783868 ID=cds.MS.gene049195.t1;Parent=MS.gene049195.t1
Gene Sequence

>MS.gene049195

ATGGATTTAGAATCAGTGAAGAGGTATTTGGAGAAAAGAGAAACATCAACGGTAGATGACGAATTTCCTCCCAAGTTTCTGGAACCTTTCATCGTGCGTGGCCTACGATTGGATCTCATTGAGCCTGGCCGTGTCGTTTTCTCAATGAACATACCTCCACGTTTACTCGTTCGTACTCTCCTCAATCTTCTTCTTCTTGCTTTTGATGATGGATTTTGTTGTTATTTTGACCTTAATTATTTAATTAACACGCAGAATTCAGGTAAGCACTTGCACGTTGGTGCCATTGTTACTCTGGTGGATGTGGTAGGAGCAATTGCAATTCCTGCCGCTGGATTTCCGATGGATACTGGAGTTTCCCTCGAGATCAATGTCTCATGCTTCGATGCTGCTTATGCCAATGTTAGTTTGTTTCATAGCTTAACCCCTCTTGTATTATCGTTAATCATACAATTTTCAATTAACATAATTGTCCCAATTAAAATTGGTTTTTCATTGTAATTAGTTGTATGACACGACATGTCCATTAAGCACATATGCTAATTCACACAAATAAAGTGAAGCTATACATAGAGAGGATGCATTTTTTACAAAGACTACTAATAAAACTTGAAATCTATGTTTTGAAAATTTCAATCCCTTTTTCGTTTCTAAATAATATTCATTGGCTTGTCAGGGTGATTGGTGTCAGACTTCATTTTATAAACGATGCCCTTAAACACTAATTTTTCTTTTAAAACAATGAATGTTTTGAAAATTTCAGCATTTTTAAACACTTTCATGATATCTTTTGTAATGTGTATTACCCGAAGAAAACGACCATTTATTTAGTCTAGACTAATAAAGGGAAAACAAAGAATGCATATTTATGCTAAAAAAAACCCAGACAAACTCTTTTTTATGATGTTTTTCACCACTGCTAATTGAATATGAGAATGAGGAAGTGAGAAGACTGAACAGAATCTTCTGAGTCCATTAGTCATTCATTGAAACTTCTTATACAAAGTATCTACACTAGCTTACTTCCCCACTAAGCTAACAGTAATGTATCATTCACTAAAAATAACAGAAAATGAATGAGTAACACTACTCCTTTCAATTTTTTTACATCTGTGATATCAAATTGATCATTAGAGGAATATGACATTCTTTTCCTTGTTTTTTGTATTAAATTTGTGTGATGAAGATATCACAAAAATAGTTCTTTTTTTGTTTAATTCCCTATGGCTTATGTAATGGTAGGAGGAGATTGAGATTGATAACAAGGTTCTACGAGTAGGAAAGGCTATAGCTGTTGTAAGCGTAGAGTTCAGAAAGAAAAAGACGGGCCAAATTTTTGCTCAAGGGCGTCATACCAAATATCTTCCCTTTGTCAGTAAAATGTGA

Protein sequence

>MS.gene049195.t1

MDLESVKRYLEKRETSTVDDEFPPKFLEPFIVRGLRLDLIEPGRVVFSMNIPPRLLNSGKHLHVGAIVTLVDVVGAIAIPAAGFPMDTGVSLEINVSCFDAAYANEEIEIDNKVLRVGKAIAVVSVEFRKKKTGQIFAQGRHTKYLPFVSKM