AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47563

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47563.t1 MTR_4g088055 100.000 196 0 0 1 196 1 196 3.04e-147 407
MS.gene47563.t1 MTR_4g073250 83.249 197 32 1 1 196 1 197 1.96e-122 344
MS.gene47563.t1 MTR_2g090875 86.735 196 26 0 1 196 1 196 4.77e-122 343
MS.gene47563.t1 MTR_8g075240 85.955 178 25 0 1 178 1 178 1.51e-116 329
MS.gene47563.t1 MTR_5g022600 84.831 178 27 0 1 178 1 178 5.34e-114 323
MS.gene47563.t1 MTR_6g087980 74.874 199 46 1 1 195 1 199 1.88e-112 319
MS.gene47563.t1 MTR_3g078260 73.980 196 50 1 2 196 4 199 1.67e-109 312
MS.gene47563.t1 MTR_3g078260 84.034 119 19 0 2 120 4 122 2.22e-73 218
MS.gene47563.t1 MTR_1g115325 34.132 167 97 5 6 170 15 170 1.41e-27 103
MS.gene47563.t1 MTR_1g068740 32.979 188 110 6 6 188 15 191 1.66e-27 103
MS.gene47563.t1 MTR_1g068730 32.979 188 110 6 6 188 15 191 3.01e-27 103
MS.gene47563.t1 MTR_7g100020 32.447 188 111 6 6 188 15 191 7.38e-27 102
MS.gene47563.t1 MTR_7g103070 33.146 178 104 5 8 181 18 184 2.02e-26 100
MS.gene47563.t1 MTR_1g094220 33.702 181 100 8 7 183 32 196 3.75e-25 97.1
MS.gene47563.t1 MTR_4g063160 33.728 169 97 6 6 170 15 172 8.10e-25 96.7
MS.gene47563.t1 MTR_2g096550 31.414 191 116 6 5 191 14 193 9.37e-25 96.7
MS.gene47563.t1 MTR_8g006970 33.333 189 107 8 6 187 22 198 2.55e-24 95.5
MS.gene47563.t1 MTR_2g027660 31.395 172 104 5 1 170 1 160 4.54e-24 94.7
MS.gene47563.t1 MTR_8g093740 30.726 179 109 5 6 181 13 179 6.16e-24 94.4
MS.gene47563.t1 MTR_8g006970 34.286 175 98 7 6 175 8 170 1.61e-23 92.4
MS.gene47563.t1 MTR_1g062760 33.333 177 102 6 8 180 16 180 2.72e-23 92.8
MS.gene47563.t1 MTR_7g081700 31.217 189 111 7 6 187 8 184 3.45e-23 92.0
MS.gene47563.t1 MTR_8g103550 33.146 178 102 6 8 183 11 173 3.51e-23 92.0
MS.gene47563.t1 MTR_1g094220 32.571 175 101 7 7 177 32 193 4.27e-23 92.0
MS.gene47563.t1 MTR_3g096350 30.337 178 109 5 7 181 13 178 5.02e-23 92.0
MS.gene47563.t1 MTR_6g021790 30.814 172 105 5 1 170 1 160 6.99e-23 91.7
MS.gene47563.t1 MTR_1g094220 33.136 169 96 7 7 171 32 187 7.05e-23 91.7
MS.gene47563.t1 MTR_1g068730 34.884 129 81 3 6 132 15 142 1.22e-22 89.7
MS.gene47563.t1 MTR_1g094220 32.967 182 102 8 7 181 32 200 1.49e-22 91.3
MS.gene47563.t1 MTR_1g068740 35.484 124 77 3 6 127 15 137 6.00e-22 89.0
MS.gene47563.t1 MTR_4g098950 35.227 176 97 6 8 181 11 171 8.63e-22 88.6
MS.gene47563.t1 MTR_2g005500 31.138 167 101 5 6 170 9 163 9.19e-22 88.6
MS.gene47563.t1 MTR_1g029450 29.609 179 111 5 6 181 17 183 1.06e-21 88.6
MS.gene47563.t1 MTR_5g013900 35.227 176 97 6 8 181 11 171 1.35e-21 88.2
MS.gene47563.t1 MTR_8g031870 29.070 172 108 5 1 170 1 160 3.34e-21 86.7
MS.gene47563.t1 MTR_3g064390 30.978 184 108 6 8 187 12 180 3.78e-21 86.7
MS.gene47563.t1 MTR_2g083860 37.500 120 72 3 6 123 13 131 4.26e-21 87.0
MS.gene47563.t1 MTR_2g005510 33.793 145 86 4 6 141 15 158 1.14e-20 85.9
MS.gene47563.t1 MTR_8g090215 33.939 165 95 5 8 170 11 163 1.17e-20 85.5
MS.gene47563.t1 MTR_3g087160 33.333 165 96 4 8 170 11 163 1.30e-20 85.5
MS.gene47563.t1 MTR_2g102060 30.909 165 100 5 8 170 12 164 1.57e-20 85.1
MS.gene47563.t1 MTR_1g052475 38.462 117 69 3 8 122 17 132 2.04e-20 85.5
MS.gene47563.t1 MTR_3g069510 32.934 167 98 5 6 170 12 166 2.32e-20 85.1
MS.gene47563.t1 MTR_5g076590 29.891 184 110 6 8 187 12 180 2.47e-20 84.7
MS.gene47563.t1 MTR_8g089985 32.121 165 98 5 8 170 19 171 2.65e-20 85.1
MS.gene47563.t1 MTR_4g079350 34.667 150 88 4 6 146 13 161 2.98e-20 84.7
MS.gene47563.t1 MTR_8g027220 38.655 119 70 3 8 124 28 145 3.38e-20 85.1
MS.gene47563.t1 MTR_2g075950 37.500 120 72 3 6 123 13 131 4.30e-20 84.3
MS.gene47563.t1 MTR_7g117910 36.508 126 77 3 8 131 22 146 5.51e-20 84.0
MS.gene47563.t1 MTR_4g088090 37.500 120 72 3 6 123 13 131 5.89e-20 84.0
MS.gene47563.t1 MTR_2g014990 37.500 120 72 3 6 123 13 131 6.01e-20 84.0
MS.gene47563.t1 MTR_4g064897 31.737 167 100 6 6 170 12 166 6.70e-20 84.0
MS.gene47563.t1 MTR_0262s0050 37.607 117 70 3 8 122 17 132 7.42e-20 84.0
MS.gene47563.t1 MTR_3g086980 30.909 165 100 5 8 170 18 170 1.07e-19 83.6
MS.gene47563.t1 MTR_8g031660 29.070 172 108 5 1 170 1 160 1.77e-19 82.4
MS.gene47563.t1 MTR_2g101890 30.556 180 110 5 6 179 10 180 4.14e-19 81.6
MS.gene47563.t1 MTR_4g069850 29.032 186 117 4 6 185 8 184 6.95e-19 80.9
MS.gene47563.t1 MTR_8g092020 29.775 178 110 5 6 177 12 180 9.18e-19 80.9
MS.gene47563.t1 MTR_8g031830 28.070 171 109 5 1 169 1 159 1.46e-18 79.7
MS.gene47563.t1 MTR_1g097420 29.688 192 118 6 1 184 1 183 1.48e-18 80.1
MS.gene47563.t1 MTR_3g064390 34.426 122 77 3 8 127 12 132 1.64e-18 79.0
MS.gene47563.t1 MTR_4g012940 29.947 187 114 6 1 179 1 178 2.51e-18 79.3
MS.gene47563.t1 MTR_1g078200 30.233 172 106 5 6 175 14 173 4.01e-18 79.3
MS.gene47563.t1 MTR_8g031680 26.744 172 112 5 1 170 1 160 4.78e-18 79.0
MS.gene47563.t1 MTR_8g036220 31.138 167 101 5 6 170 16 170 5.85e-18 79.0
MS.gene47563.t1 MTR_7g051940 30.233 172 106 5 6 175 13 172 7.95e-18 78.2
MS.gene47563.t1 MTR_3g096350 33.051 118 75 3 7 121 13 129 1.54e-17 76.3
MS.gene47563.t1 MTR_1g090717 29.891 184 112 7 8 186 17 188 1.54e-17 77.4
MS.gene47563.t1 MTR_3g096350 33.333 117 74 3 8 121 14 129 1.89e-17 75.9
MS.gene47563.t1 MTR_8g107070 34.454 119 75 3 6 122 14 131 6.82e-17 76.6
MS.gene47563.t1 MTR_7g103070 32.609 138 79 4 47 181 2 128 1.53e-16 73.6
MS.gene47563.t1 MTR_8g093740 28.395 162 92 4 22 181 7 146 1.88e-16 73.9
MS.gene47563.t1 MTR_7g405790 29.070 172 108 5 6 175 15 174 2.35e-16 74.3
MS.gene47563.t1 MTR_2g090865 36.667 120 73 3 6 123 11 129 3.62e-16 73.9
MS.gene47563.t1 MTR_6g079550 33.065 124 80 3 6 127 12 134 1.50e-15 72.4
MS.gene47563.t1 MTR_4g099470 30.822 146 87 5 29 172 7 140 2.30e-15 70.9
MS.gene47563.t1 MTR_1g097420 29.310 174 108 5 17 184 46 210 3.54e-15 71.6
MS.gene47563.t1 MTR_7g094810 26.842 190 105 3 7 183 9 177 1.97e-14 71.2
MS.gene47563.t1 MTR_3g107707 27.879 165 104 4 7 170 14 164 2.11e-14 69.3
MS.gene47563.t1 MTR_3g107717 27.879 165 104 4 7 170 14 164 2.18e-14 69.3
MS.gene47563.t1 MTR_3g107713 27.879 165 104 4 7 170 14 164 2.27e-14 69.3
MS.gene47563.t1 MTR_7g094810 26.842 190 105 3 7 183 9 177 2.41e-14 70.9
MS.gene47563.t1 MTR_7g094810 26.842 190 105 3 7 183 9 177 2.42e-14 70.9
MS.gene47563.t1 MTR_7g094810 26.842 190 105 3 7 183 9 177 2.47e-14 70.9
MS.gene47563.t1 MTR_7g094810 26.842 190 105 3 7 183 9 177 2.48e-14 70.9
MS.gene47563.t1 MTR_1g072280 28.652 178 117 4 7 183 13 181 1.99e-13 68.2
MS.gene47563.t1 MTR_1g082870 28.090 178 118 5 7 183 17 185 1.71e-12 65.5
MS.gene47563.t1 MTR_4g113620 25.610 164 100 5 7 170 14 155 1.25e-11 61.6
MS.gene47563.t1 MTR_6g039490 25.405 185 123 4 6 184 8 183 1.59e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47563.t1 AT5G45970 82.090 201 31 1 1 196 1 201 1.78e-120 339
MS.gene47563.t1 AT4G35020 85.083 181 27 0 1 181 1 181 2.72e-119 336
MS.gene47563.t1 AT4G35020 85.083 181 27 0 1 181 1 181 2.72e-119 336
MS.gene47563.t1 AT4G35020 85.083 181 27 0 1 181 1 181 2.72e-119 336
MS.gene47563.t1 AT1G75840 84.694 196 30 0 1 196 1 196 2.22e-118 334
MS.gene47563.t1 AT1G20090 85.492 193 28 0 4 196 3 195 9.02e-118 332
MS.gene47563.t1 AT3G51300 87.079 178 23 0 1 178 1 178 2.80e-117 331
MS.gene47563.t1 AT2G17800 87.640 178 22 0 1 178 1 178 2.83e-117 331
MS.gene47563.t1 AT2G17800 87.640 178 22 0 1 178 1 178 2.83e-117 331
MS.gene47563.t1 AT4G35950 86.517 178 24 0 1 178 1 178 4.44e-116 328
MS.gene47563.t1 AT4G28950 75.393 191 45 1 1 191 1 189 3.75e-108 308
MS.gene47563.t1 AT5G62880 78.212 179 39 0 2 180 4 182 9.73e-108 308
MS.gene47563.t1 AT3G48040 77.654 179 40 0 2 180 4 182 1.38e-106 305
MS.gene47563.t1 AT2G44690 75.000 192 47 1 6 196 18 209 7.78e-105 300
MS.gene47563.t1 AT5G62880 77.326 172 35 1 2 173 4 171 2.25e-100 288
MS.gene47563.t1 AT3G48040 78.528 163 35 0 2 164 4 166 1.53e-97 280
MS.gene47563.t1 AT3G51290 86.897 145 19 0 34 178 635 779 2.01e-87 273
MS.gene47563.t1 AT4G35020 89.167 120 13 0 1 120 1 120 1.62e-79 234
MS.gene47563.t1 AT4G35020 89.167 120 13 0 1 120 1 120 1.62e-79 234
MS.gene47563.t1 AT3G46060 34.132 167 97 5 6 170 15 170 2.63e-27 103
MS.gene47563.t1 AT3G46060 34.132 167 97 5 6 170 15 170 2.63e-27 103
MS.gene47563.t1 AT3G46060 34.132 167 97 5 6 170 15 170 2.63e-27 103
MS.gene47563.t1 AT3G54840 33.333 180 102 6 7 183 34 198 7.90e-27 101
MS.gene47563.t1 AT3G54840 33.146 178 101 6 9 183 2 164 2.70e-26 99.4
MS.gene47563.t1 AT5G59840 32.934 167 99 5 6 170 15 170 4.72e-26 100
MS.gene47563.t1 AT3G54840 33.333 168 97 5 7 171 34 189 2.42e-25 97.4
MS.gene47563.t1 AT3G53610 32.335 167 100 5 6 170 15 170 7.79e-25 97.1
MS.gene47563.t1 AT3G53610 32.335 167 100 5 6 170 15 170 7.79e-25 97.1
MS.gene47563.t1 AT3G53610 32.335 167 100 5 6 170 15 170 7.79e-25 97.1
MS.gene47563.t1 AT5G45130 32.609 184 105 6 8 187 12 180 1.23e-24 95.9
MS.gene47563.t1 AT4G35860 31.844 179 107 6 1 176 1 167 2.63e-24 95.5
MS.gene47563.t1 AT4G19640 32.609 184 105 6 8 187 12 180 3.52e-24 94.7
MS.gene47563.t1 AT4G17170 31.395 172 104 5 1 170 1 160 3.61e-24 95.1
MS.gene47563.t1 AT5G45130 32.418 182 104 6 10 187 22 188 5.74e-24 94.4
MS.gene47563.t1 AT1G02130 32.275 189 109 8 6 187 8 184 1.01e-23 93.6
MS.gene47563.t1 AT4G17530 32.275 189 109 7 6 187 8 184 1.47e-23 93.2
MS.gene47563.t1 AT5G47200 32.275 189 109 7 6 187 8 184 3.32e-23 92.4
MS.gene47563.t1 AT4G17160 30.233 172 106 5 1 170 1 160 3.44e-23 92.4
MS.gene47563.t1 AT2G44610 35.795 176 96 6 8 181 11 171 9.42e-23 91.3
MS.gene47563.t1 AT1G43890 30.168 179 110 5 6 181 13 179 1.05e-22 91.3
MS.gene47563.t1 AT1G43890 30.168 179 110 5 6 181 13 179 1.05e-22 91.3
MS.gene47563.t1 AT1G43890 30.168 179 110 5 6 181 13 179 1.05e-22 91.3
MS.gene47563.t1 AT5G03520 33.511 188 109 6 6 188 15 191 2.04e-22 90.5
MS.gene47563.t1 AT3G09900 34.132 167 97 5 6 170 15 170 2.34e-22 90.5
MS.gene47563.t1 AT1G09630 35.333 150 87 4 6 146 12 160 3.24e-22 90.1
MS.gene47563.t1 AT4G39890 35.294 170 94 6 4 170 8 164 3.42e-22 89.7
MS.gene47563.t1 AT1G16920 39.167 120 70 3 6 123 13 131 4.74e-22 89.7
MS.gene47563.t1 AT1G18200 33.333 171 100 5 6 174 13 171 6.78e-22 89.4
MS.gene47563.t1 AT2G22290 36.364 165 91 5 8 170 11 163 1.44e-21 88.2
MS.gene47563.t1 AT5G03530 33.708 178 102 8 8 181 15 180 1.91e-21 87.8
MS.gene47563.t1 AT5G03530 33.708 178 102 8 8 181 15 180 1.91e-21 87.8
MS.gene47563.t1 AT3G12160 39.316 117 68 3 8 122 17 132 4.99e-21 87.0
MS.gene47563.t1 AT5G45750 33.533 167 97 5 6 170 13 167 6.33e-21 86.7
MS.gene47563.t1 AT1G06400 38.333 120 71 3 6 123 13 131 6.40e-21 86.7
MS.gene47563.t1 AT3G11730 29.940 167 103 5 6 170 8 162 6.71e-21 86.3
MS.gene47563.t1 AT3G18820 30.645 186 114 5 6 185 8 184 9.60e-21 85.9
MS.gene47563.t1 AT1G73640 33.533 167 97 5 6 170 13 167 9.97e-21 86.7
MS.gene47563.t1 AT5G10260 34.545 165 94 5 8 170 11 163 1.16e-20 85.9
MS.gene47563.t1 AT4G19640 36.066 122 75 3 8 127 12 132 1.67e-20 84.0
MS.gene47563.t1 AT1G22740 31.250 192 115 6 1 184 1 183 1.68e-20 85.1
MS.gene47563.t1 AT4G18430 40.000 120 69 3 6 123 13 131 1.99e-20 85.5
MS.gene47563.t1 AT3G15060 38.983 118 69 3 8 123 15 131 3.15e-20 84.7
MS.gene47563.t1 AT4G19640 36.066 122 75 3 8 127 12 132 5.19e-20 83.6
MS.gene47563.t1 AT4G18800 32.335 167 99 5 6 170 13 167 6.53e-20 84.0
MS.gene47563.t1 AT5G59150 30.994 171 104 5 6 174 12 170 8.02e-20 83.6
MS.gene47563.t1 AT5G64990 35.152 165 93 5 8 170 9 161 1.17e-19 83.2
MS.gene47563.t1 AT5G60860 37.500 120 72 3 6 123 13 131 1.87e-19 82.8
MS.gene47563.t1 AT5G65270 30.097 206 123 6 8 193 19 223 2.06e-19 82.8
MS.gene47563.t1 AT1G28550 38.333 120 71 3 6 123 13 131 2.19e-19 82.4
MS.gene47563.t1 AT1G07410 31.138 167 101 5 6 170 12 166 2.83e-19 82.0
MS.gene47563.t1 AT1G52280 29.744 195 119 7 1 187 1 185 4.74e-19 81.3
MS.gene47563.t1 AT2G33870 38.655 119 70 3 6 122 13 130 6.09e-19 81.3
MS.gene47563.t1 AT5G47960 35.043 117 73 3 8 122 17 132 6.40e-19 81.6
MS.gene47563.t1 AT5G47520 29.651 172 107 5 6 175 14 173 7.69e-19 81.3
MS.gene47563.t1 AT4G39990 33.884 121 77 3 8 126 19 138 8.46e-19 81.3
MS.gene47563.t1 AT3G46830 30.539 167 102 5 6 170 12 166 1.10e-18 80.9
MS.gene47563.t1 AT3G46830 30.539 167 102 5 6 170 12 166 1.10e-18 80.9
MS.gene47563.t1 AT2G43130 29.651 172 107 5 6 175 12 171 1.22e-18 80.5
MS.gene47563.t1 AT2G31680 29.651 172 107 5 6 175 12 171 3.47e-18 79.3
MS.gene47563.t1 AT2G21880 28.649 185 117 5 6 184 9 184 5.53e-18 78.6
MS.gene47563.t1 AT3G16100 30.481 187 113 6 1 179 1 178 6.09e-18 78.6
MS.gene47563.t1 AT1G05810 29.240 171 107 5 6 174 12 170 8.78e-18 78.2
MS.gene47563.t1 AT1G05810 29.240 171 107 5 6 174 58 216 1.14e-17 79.0
MS.gene47563.t1 AT1G01200 35.897 117 72 3 8 122 30 145 1.34e-17 78.2
MS.gene47563.t1 AT5G64990 33.721 172 93 6 8 170 9 168 6.04e-17 75.9
MS.gene47563.t1 AT1G49300 29.825 171 105 5 6 170 8 169 6.56e-17 75.9
MS.gene47563.t1 AT1G49300 29.825 171 105 5 6 170 8 169 6.56e-17 75.9
MS.gene47563.t1 AT3G07410 29.651 172 107 5 6 175 12 171 7.72e-17 75.9
MS.gene47563.t1 AT4G09720 29.032 186 115 6 1 178 1 177 1.43e-16 74.7
MS.gene47563.t1 AT5G03520 33.562 146 82 5 47 188 47 181 1.95e-16 74.3
MS.gene47563.t1 AT4G09720 28.272 191 115 5 1 178 1 182 3.07e-16 73.9
MS.gene47563.t1 AT4G35860 31.818 132 76 5 47 176 2 121 4.56e-16 72.4
MS.gene47563.t1 AT5G55080 32.479 117 77 2 7 122 14 129 1.51e-15 72.4
MS.gene47563.t1 AT4G09720 27.411 197 115 5 1 178 1 188 2.56e-15 71.6
MS.gene47563.t1 AT3G09910 27.228 202 120 7 8 194 15 204 5.33e-15 70.5
MS.gene47563.t1 AT3G09910 27.228 202 120 7 8 194 15 204 5.33e-15 70.5
MS.gene47563.t1 AT3G09910 27.228 202 120 7 8 194 15 204 5.33e-15 70.5
MS.gene47563.t1 AT5G27540 24.859 177 125 3 7 183 18 186 7.50e-15 72.4
MS.gene47563.t1 AT5G27540 24.859 177 125 3 7 183 18 186 7.50e-15 72.4
MS.gene47563.t1 AT5G39620 29.213 178 111 5 7 178 6 174 1.75e-14 69.3
MS.gene47563.t1 AT5G20020 27.879 165 104 4 7 170 14 164 2.08e-14 69.3
MS.gene47563.t1 AT5G20010 27.879 165 104 4 7 170 14 164 2.19e-14 69.3
MS.gene47563.t1 AT5G55190 27.879 165 104 4 7 170 14 164 2.19e-14 69.3
MS.gene47563.t1 AT3G09910 28.571 189 117 7 8 190 15 191 2.49e-14 68.6
MS.gene47563.t1 AT3G09910 28.571 189 117 7 8 190 15 191 2.49e-14 68.6
MS.gene47563.t1 AT3G63150 28.652 178 117 4 7 183 15 183 1.52e-13 68.9
MS.gene47563.t1 AT5G59150 30.233 129 77 4 47 174 3 119 5.17e-13 64.3
MS.gene47563.t1 AT2G21880 27.381 168 107 5 23 184 1 159 5.72e-12 62.0
MS.gene47563.t1 AT2G21880 27.381 168 107 5 23 184 1 159 5.72e-12 62.0

Find 35 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACAGTTAATCTTGGTTTA+TGG 0.192232 4.3:-25383481 MS.gene47563:CDS
GTCTTGCGTGGTTTCTTCTT+TGG 0.222979 4.3:+25382490 None:intergenic
GTGCTGGTGGGAACAAAATT+AGG 0.286733 4.3:-25383084 MS.gene47563:intron
CGTGGTTTCTTCTTTGGCTT+TGG 0.304527 4.3:+25382496 None:intergenic
TTATGAGAACATTTCCAAAA+AGG 0.315063 4.3:-25383235 MS.gene47563:intron
TACAGTTAATCTTGGTTTAT+GGG 0.333419 4.3:-25383480 MS.gene47563:CDS
AAGAAGATTACAATAGATTA+AGG 0.345982 4.3:-25383320 MS.gene47563:CDS
TTTGTTCCCACCAGCACAAT+AGG 0.364490 4.3:+25383090 None:intergenic
ACCAGCACAATAGGCACATT+TGG 0.392174 4.3:+25383099 None:intergenic
GATGGTAGTACAGTTAATCT+TGG 0.405802 4.3:-25383488 MS.gene47563:CDS
GGTTTCTTCTTTGGCTTTGG+TGG 0.414729 4.3:+25382499 None:intergenic
GAAGAATTAAAGAAAATGAT+TGG 0.437849 4.3:-25382758 MS.gene47563:CDS
GGGAAAGGTATTGCTTGTAT+AGG 0.469336 4.3:+25383649 None:intergenic
TCCAAATGTGCCTATTGTGC+TGG 0.495554 4.3:-25383100 MS.gene47563:CDS
GTTGTTATTTGTGTTGCTCC+AGG 0.503224 4.3:+25382898 None:intergenic
CTTGGTTTATGGGATACTGC+AGG 0.516373 4.3:-25383470 MS.gene47563:intron
CTGAAATTGTCAAACACAGT+TGG 0.527759 4.3:+25383527 None:intergenic
TTTATCAAGTGTGTCACAGT+AGG 0.541993 4.3:-25383707 MS.gene47563:CDS
TTAGAAACATACTGTGGGAA+AGG 0.550222 4.3:+25383634 None:intergenic
TTATCAAGTGTGTCACAGTA+GGG 0.554079 4.3:-25383706 MS.gene47563:CDS
ACAGTAGGGGATGGTGCTGT+TGG 0.554138 4.3:-25383692 MS.gene47563:CDS
AGAAGAAACCACGCAAGACA+AGG 0.561090 4.3:-25382486 MS.gene47563:CDS
AAATGTGCCTATTGTGCTGG+TGG 0.561822 4.3:-25383097 MS.gene47563:CDS
TCAGCTCCTCTGTAACTCAA+TGG 0.570878 4.3:+25383297 None:intergenic
AACACAAATAACAACTGCTA+AGG 0.570922 4.3:-25382890 MS.gene47563:intron
GTACATGTCCTTGTCTTGCG+TGG 0.584257 4.3:+25382478 None:intergenic
GTGCAGCTCTAAAACACAGC+AGG 0.601225 4.3:-25382717 MS.gene47563:intron
TTAAGGCCATTGAGTTACAG+AGG 0.604328 4.3:-25383303 MS.gene47563:CDS
AAGTGTGTCACAGTAGGGGA+TGG 0.619947 4.3:-25383701 MS.gene47563:CDS
AGTGCTAATGTAGTGGTGGA+TGG 0.631459 4.3:-25383506 MS.gene47563:CDS
TATCAAGTGTGTCACAGTAG+GGG 0.650658 4.3:-25383705 MS.gene47563:CDS
CAATTTCAGTGCTAATGTAG+TGG 0.654836 4.3:-25383513 MS.gene47563:CDS
TTTCAGTGCTAATGTAGTGG+TGG 0.655908 4.3:-25383510 MS.gene47563:CDS
AATGTGCCTATTGTGCTGGT+GGG 0.668934 4.3:-25383096 MS.gene47563:CDS
CTGCAATAAAGATTGCATTG+AGG 0.683742 4.3:-25382522 MS.gene47563:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTAATCAATGATTG+TGG - chr4.3:25383399-25383418 MS.gene47563:intron 15.0%
!!! AATTTTTTGAGGTTATTTAT+TGG - chr4.3:25382823-25382842 MS.gene47563:intron 15.0%
!! AAGAAGATTACAATAGATTA+AGG - chr4.3:25382870-25382889 MS.gene47563:intron 20.0%
!! ATTACTGTCAACTTATAATA+TGG + chr4.3:25383164-25383183 None:intergenic 20.0%
!! GAAGAATTAAAGAAAATGAT+TGG - chr4.3:25383432-25383451 MS.gene47563:intron 20.0%
!! TTCAAAAAATACTTCACAAT+TGG + chr4.3:25383592-25383611 None:intergenic 20.0%
!! TTGTACATGCAAAATAATAT+TGG + chr4.3:25383373-25383392 None:intergenic 20.0%
!!! ATTTTGTCTGAAATTTTTTG+AGG - chr4.3:25382812-25382831 MS.gene47563:intron 20.0%
!!! TATTATTTTGCATGTACAAA+TGG - chr4.3:25383374-25383393 MS.gene47563:intron 20.0%
!!! TCTTTTGATTTTTCTATGAT+AGG - chr4.3:25382632-25382651 MS.gene47563:intron 20.0%
! AACTATTTAGAAACATACTG+TGG + chr4.3:25382565-25382584 None:intergenic 25.0%
! ACTATTTAGAAACATACTGT+GGG + chr4.3:25382564-25382583 None:intergenic 25.0%
! ACTTATAAGGTAATGTATAG+AGG + chr4.3:25382989-25383008 None:intergenic 25.0%
! ATTTATTGGTGTAAATTGTG+TGG - chr4.3:25382837-25382856 MS.gene47563:intron 25.0%
! GAGATCTTATCTAACTTATA+AGG + chr4.3:25383002-25383021 None:intergenic 25.0%
! TACAGTTAATCTTGGTTTAT+GGG - chr4.3:25382710-25382729 MS.gene47563:intron 25.0%
! TTATGAGAACATTTCCAAAA+AGG - chr4.3:25382955-25382974 MS.gene47563:intron 25.0%
!! TAGAGGAAAAAAAACCTTTT+TGG + chr4.3:25382972-25382991 None:intergenic 25.0%
!!! AAATTACAGCACTTCTTTTT+TGG - chr4.3:25383501-25383520 MS.gene47563:CDS 25.0%
!!! CATTTTTAGTTGATTAACAG+TGG - chr4.3:25383044-25383063 MS.gene47563:intron 25.0%
AACACAAATAACAACTGCTA+AGG - chr4.3:25383300-25383319 MS.gene47563:CDS 30.0%
AATCAATGATTGTGGTAAAC+AGG - chr4.3:25383407-25383426 MS.gene47563:intron 30.0%
ACTGTCAACTTATAATATGG+TGG + chr4.3:25383161-25383180 None:intergenic 30.0%
ATCAATGATTGTGGTAAACA+GGG - chr4.3:25383408-25383427 MS.gene47563:intron 30.0%
GTACAGTTAATCTTGGTTTA+TGG - chr4.3:25382709-25382728 MS.gene47563:intron 30.0%
! CAATTTTTCATTGATCATCC+TGG - chr4.3:25383274-25383293 MS.gene47563:CDS 30.0%
! TGGTTGAAATGTTACTTAGA+TGG + chr4.3:25383141-25383160 None:intergenic 30.0%
!! ATCAATGTCAAGTTTTGATG+AGG - chr4.3:25382785-25382804 MS.gene47563:intron 30.0%
!!! GTTTTGTTTACAGAATGTGA+AGG - chr4.3:25383633-25383652 MS.gene47563:intron 30.0%
CAATTTCAGTGCTAATGTAG+TGG - chr4.3:25382677-25382696 MS.gene47563:intron 35.0%
CTGAAATTGTCAAACACAGT+TGG + chr4.3:25382666-25382685 None:intergenic 35.0%
CTGCAATAAAGATTGCATTG+AGG - chr4.3:25383668-25383687 MS.gene47563:CDS 35.0%
GATGGTAGTACAGTTAATCT+TGG - chr4.3:25382702-25382721 MS.gene47563:intron 35.0%
TTAGAAACATACTGTGGGAA+AGG + chr4.3:25382559-25382578 None:intergenic 35.0%
TTATCAAGTGTGTCACAGTA+GGG - chr4.3:25382484-25382503 MS.gene47563:CDS 35.0%
TTTATCAAGTGTGTCACAGT+AGG - chr4.3:25382483-25382502 MS.gene47563:CDS 35.0%
! GTGTAAATTGTGTGGATTAC+AGG - chr4.3:25382845-25382864 MS.gene47563:intron 35.0%
TATCAAGTGTGTCACAGTAG+GGG - chr4.3:25382485-25382504 MS.gene47563:CDS 40.0%
TCGCAAATCTGTGCATACAT+AGG + chr4.3:25383245-25383264 None:intergenic 40.0%
TTAAGGCCATTGAGTTACAG+AGG - chr4.3:25382887-25382906 MS.gene47563:intron 40.0%
TTTCAGTGCTAATGTAGTGG+TGG - chr4.3:25382680-25382699 MS.gene47563:intron 40.0%
!! GGGAAAGGTATTGCTTGTAT+AGG + chr4.3:25382544-25382563 None:intergenic 40.0%
!! GTTGTTATTTGTGTTGCTCC+AGG + chr4.3:25383295-25383314 None:intergenic 40.0%
AAATGTGCCTATTGTGCTGG+TGG - chr4.3:25383093-25383112 MS.gene47563:CDS 45.0%
ACCAGCACAATAGGCACATT+TGG + chr4.3:25383094-25383113 None:intergenic 45.0%
AGAAGAAACCACGCAAGACA+AGG - chr4.3:25383704-25383723 MS.gene47563:CDS 45.0%
CTTGGTTTATGGGATACTGC+AGG - chr4.3:25382720-25382739 MS.gene47563:CDS 45.0%
GTCTTGCGTGGTTTCTTCTT+TGG + chr4.3:25383703-25383722 None:intergenic 45.0%
TCAGCTCCTCTGTAACTCAA+TGG + chr4.3:25382896-25382915 None:intergenic 45.0%
TCCAAATGTGCCTATTGTGC+TGG - chr4.3:25383090-25383109 MS.gene47563:CDS 45.0%
TTTGTTCCCACCAGCACAAT+AGG + chr4.3:25383103-25383122 None:intergenic 45.0%
! AATGTGCCTATTGTGCTGGT+GGG - chr4.3:25383094-25383113 MS.gene47563:CDS 45.0%
! AGTGCTAATGTAGTGGTGGA+TGG - chr4.3:25382684-25382703 MS.gene47563:intron 45.0%
! CGTGGTTTCTTCTTTGGCTT+TGG + chr4.3:25383697-25383716 None:intergenic 45.0%
! GTGCTGGTGGGAACAAAATT+AGG - chr4.3:25383106-25383125 MS.gene47563:CDS 45.0%
!! GGTTTCTTCTTTGGCTTTGG+TGG + chr4.3:25383694-25383713 None:intergenic 45.0%
AAGTGTGTCACAGTAGGGGA+TGG - chr4.3:25382489-25382508 MS.gene47563:CDS 50.0%
GTACATGTCCTTGTCTTGCG+TGG + chr4.3:25383715-25383734 None:intergenic 50.0%
!! GTGCAGCTCTAAAACACAGC+AGG - chr4.3:25383473-25383492 MS.gene47563:CDS 50.0%
!! ACAGTAGGGGATGGTGCTGT+TGG - chr4.3:25382498-25382517 MS.gene47563:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 25382468 25383744 25382468 ID=MS.gene47563
chr4.3 mRNA 25382468 25383744 25382468 ID=MS.gene47563.t1;Parent=MS.gene47563
chr4.3 exon 25383646 25383744 25383646 ID=MS.gene47563.t1.exon1;Parent=MS.gene47563.t1
chr4.3 CDS 25383646 25383744 25383646 ID=cds.MS.gene47563.t1;Parent=MS.gene47563.t1
chr4.3 exon 25383471 25383558 25383471 ID=MS.gene47563.t1.exon2;Parent=MS.gene47563.t1
chr4.3 CDS 25383471 25383558 25383471 ID=cds.MS.gene47563.t1;Parent=MS.gene47563.t1
chr4.3 exon 25383236 25383345 25383236 ID=MS.gene47563.t1.exon3;Parent=MS.gene47563.t1
chr4.3 CDS 25383236 25383345 25383236 ID=cds.MS.gene47563.t1;Parent=MS.gene47563.t1
chr4.3 exon 25383085 25383148 25383085 ID=MS.gene47563.t1.exon4;Parent=MS.gene47563.t1
chr4.3 CDS 25383085 25383148 25383085 ID=cds.MS.gene47563.t1;Parent=MS.gene47563.t1
chr4.3 exon 25382891 25382955 25382891 ID=MS.gene47563.t1.exon5;Parent=MS.gene47563.t1
chr4.3 CDS 25382891 25382955 25382891 ID=cds.MS.gene47563.t1;Parent=MS.gene47563.t1
chr4.3 exon 25382718 25382783 25382718 ID=MS.gene47563.t1.exon6;Parent=MS.gene47563.t1
chr4.3 CDS 25382718 25382783 25382718 ID=cds.MS.gene47563.t1;Parent=MS.gene47563.t1
chr4.3 exon 25382468 25382566 25382468 ID=MS.gene47563.t1.exon7;Parent=MS.gene47563.t1
chr4.3 CDS 25382468 25382566 25382468 ID=cds.MS.gene47563.t1;Parent=MS.gene47563.t1
Gene Sequence

>MS.gene47563

ATGAGTACAGCTAGATTTATCAAGTGTGTCACAGTAGGGGATGGTGCTGTTGGAAAGACTTGCATGCTTATATCCTATACAAGCAATACCTTTCCCACAGTATGTTTCTAAATAGTTCAATTTACATGTAAAAAAATATAGTGATGAATCTAATATATTTTTTTTCTTTTGATTTTTCTATGATAGGATTATGTTCCAACTGTGTTTGACAATTTCAGTGCTAATGTAGTGGTGGATGGTAGTACAGTTAATCTTGGTTTATGGGATACTGCAGGTTAGTCTAAATCTCTATGTATTACTTTTAAATTTTAATATAAATCAATGTCAAGTTTTGATGAGGTGAAATTTTGTCTGAAATTTTTTGAGGTTATTTATTGGTGTAAATTGTGTGGATTACAGGACAAGAAGATTACAATAGATTAAGGCCATTGAGTTACAGAGGAGCTGATGTGTTTTTGTTGTGTTTTTCTCTCATTAGTAAAGCTAGTTATGAGAACATTTCCAAAAAGGTTTTTTTTCCTCTATACATTACCTTATAAGTTAGATAAGATCTCTATTTTTTTGTTATTGTAATAACATTTTTAGTTGATTAACAGTGGATATCTGAGCTGAGACATTATGCTCCAAATGTGCCTATTGTGCTGGTGGGAACAAAATTAGGTAAGCTATTCCATCTAAGTAACATTTCAACCACCATATTATAAGTTGACAGTAATGAAAATTTCATCTGATTATAGCTTACAGAACATTCAAAATGTCTGATTATGTTTAAATCCTATGTATGCACAGATTTGCGAGACGACAAGCAATTTTTCATTGATCATCCTGGAGCAACACAAATAACAACTGCTAAGGCATGTCGTCGATAAATAAATTATTCACAAAAATTTCTATATTTGAAACCAATATTATTTTGCATGTACAAATGGATTTAAATTTAATCAATGATTGTGGTAAACAGGGTGAAGAATTAAAGAAAATGATTGGAGCAGTTAGTTACATTGAGTGCAGCTCTAAAACACAGCAGGTAAAAAAATTACAGCACTTCTTTTTTGGATTTAACTTTTTTAGTGTGTCATATTTTTGATGCAAAAGATGCTACTTGATTCAATTATTATATTCCAATTGTGAAGTATTTTTTGAAGTATTTGTTCTAATTATTTTCGTTTTGTTTACAGAATGTGAAGGTAGTTTTTGATGCTGCAATAAAGATTGCATTGAGGCCACCAAAGCCAAAGAAGAAACCACGCAAGACAAGGACATGTACTTTCCTCTAA
Protein sequence

>MS.gene47563.t1

MSTARFIKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFSANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLCFSLISKASYENISKKWISELRHYAPNVPIVLVGTKLDLRDDKQFFIDHPGATQITTAKGEELKKMIGAVSYIECSSKTQQNVKVVFDAAIKIALRPPKPKKKPRKTRTCTFL