AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47577

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47577.t1 MTR_4g088190 98.901 364 3 1 1 363 1 364 0.0 733
MS.gene47577.t1 MTR_4g088200 89.256 363 37 1 1 363 1 361 0.0 666
MS.gene47577.t1 MTR_4g033015 75.549 364 87 2 1 363 1 363 0.0 546
MS.gene47577.t1 MTR_4g033200 75.275 364 88 2 1 363 1 363 0.0 544
MS.gene47577.t1 MTR_4g033150 74.725 364 90 2 1 363 1 363 0.0 542
MS.gene47577.t1 MTR_4g033170 74.931 363 85 1 1 363 1 357 0.0 525
MS.gene47577.t1 MTR_4g033085 75.482 363 83 1 1 363 1 357 0.0 512
MS.gene47577.t1 MTR_0794s0020 73.200 250 65 2 1 249 1 249 1.86e-133 381
MS.gene47577.t1 MTR_7g014510 49.862 363 176 4 1 363 1 357 2.72e-122 357
MS.gene47577.t1 MTR_7g014570 49.862 363 171 5 1 363 1 352 4.43e-122 356
MS.gene47577.t1 MTR_5g074600 49.859 355 170 5 11 363 11 359 3.90e-121 354
MS.gene47577.t1 MTR_4g088345 52.473 364 164 5 1 363 1 356 8.59e-120 350
MS.gene47577.t1 MTR_7g024500 48.876 356 170 5 6 361 5 348 6.90e-118 345
MS.gene47577.t1 MTR_5g074680 48.023 354 176 5 11 362 12 359 2.38e-117 344
MS.gene47577.t1 MTR_1g076940 49.180 366 178 6 1 363 1 361 1.76e-116 342
MS.gene47577.t1 MTR_7g024520 50.000 356 166 5 6 361 5 348 1.22e-115 340
MS.gene47577.t1 MTR_5g074580 48.732 355 174 5 11 363 12 360 1.75e-115 340
MS.gene47577.t1 MTR_4g079160 46.610 354 180 3 11 363 10 355 5.79e-108 320
MS.gene47577.t1 MTR_4g088340 51.320 341 157 5 24 363 1 333 9.01e-108 319
MS.gene47577.t1 MTR_5g074610 45.892 353 158 3 11 363 7 326 1.64e-106 315
MS.gene47577.t1 MTR_5g074540 45.352 355 168 5 11 363 11 341 1.35e-105 314
MS.gene47577.t1 MTR_4g038440 34.332 367 225 9 2 363 13 368 6.29e-62 202
MS.gene47577.t1 MTR_3g083620 36.152 343 204 9 20 359 27 357 1.03e-60 199
MS.gene47577.t1 MTR_0017s0100 34.153 366 227 8 2 363 13 368 4.70e-60 198
MS.gene47577.t1 MTR_0079s0070 34.503 342 208 9 27 363 4 334 1.01e-57 191
MS.gene47577.t1 MTR_1g076930 41.975 243 132 5 24 263 1 237 1.57e-55 182
MS.gene47577.t1 MTR_1g036460 31.778 343 210 10 33 362 38 369 8.68e-44 155
MS.gene47577.t1 MTR_1g036490 32.153 339 214 8 33 362 38 369 1.33e-43 155
MS.gene47577.t1 MTR_5g098170 34.139 331 200 10 28 350 30 350 2.73e-43 154
MS.gene47577.t1 MTR_7g012090 31.343 335 213 8 26 350 34 361 1.61e-41 149
MS.gene47577.t1 MTR_3g092900 32.486 354 205 11 9 349 17 349 2.52e-41 149
MS.gene47577.t1 MTR_1g096310 31.233 365 226 10 4 358 16 365 2.77e-40 146
MS.gene47577.t1 MTR_8g068750 31.714 350 215 10 9 348 27 362 3.66e-40 146
MS.gene47577.t1 MTR_7g012070 30.149 335 217 8 26 350 34 361 7.81e-40 145
MS.gene47577.t1 MTR_7g012040 30.699 329 217 7 26 350 33 354 1.21e-39 144
MS.gene47577.t1 MTR_7g118320 33.033 333 202 13 26 348 26 347 1.24e-39 144
MS.gene47577.t1 MTR_2g072790 33.141 347 204 11 26 360 21 351 2.56e-39 143
MS.gene47577.t1 MTR_7g011990 28.736 348 222 9 26 362 34 366 4.17e-39 143
MS.gene47577.t1 MTR_8g068690 32.822 326 204 9 26 344 40 357 5.60e-39 142
MS.gene47577.t1 MTR_7g011900 30.909 330 216 7 26 350 34 356 7.37e-39 142
MS.gene47577.t1 MTR_0591s0010 31.790 324 206 9 28 344 43 358 1.73e-38 141
MS.gene47577.t1 MTR_8g068870 30.423 355 229 10 4 348 2 348 1.01e-37 139
MS.gene47577.t1 MTR_7g118300 30.792 341 211 10 26 355 42 368 1.63e-36 136
MS.gene47577.t1 MTR_2g055940 31.269 323 207 10 32 348 34 347 1.65e-36 135
MS.gene47577.t1 MTR_7g012020 29.394 330 221 7 26 350 33 355 8.59e-35 131
MS.gene47577.t1 MTR_8g024160 40.541 185 105 3 173 355 140 321 1.29e-34 130
MS.gene47577.t1 MTR_4g038440 32.707 266 167 6 2 263 13 270 1.39e-34 128
MS.gene47577.t1 MTR_1g036510 30.476 315 200 9 56 362 80 383 2.35e-34 130
MS.gene47577.t1 MTR_3g021430 30.899 356 217 11 13 358 14 350 5.88e-34 129
MS.gene47577.t1 MTR_3g021440 31.548 336 198 14 32 353 42 359 2.49e-33 127
MS.gene47577.t1 MTR_3g096050 29.204 339 220 11 32 362 31 357 5.19e-32 124
MS.gene47577.t1 MTR_8g068570 28.981 314 177 8 27 331 42 318 2.87e-30 118
MS.gene47577.t1 MTR_6g078850 28.161 348 230 9 9 348 33 368 1.70e-29 119
MS.gene47577.t1 MTR_8g068730 28.571 350 194 10 9 348 27 330 1.16e-28 114
MS.gene47577.t1 MTR_3g096070 26.786 336 205 10 32 360 31 332 3.40e-25 104
MS.gene47577.t1 MTR_7g012020 29.555 247 165 6 26 267 33 275 8.13e-23 97.4
MS.gene47577.t1 MTR_1g050220 33.766 154 89 2 194 347 147 287 1.04e-20 91.7
MS.gene47577.t1 MTR_8g068690 28.745 247 165 7 26 266 40 281 6.85e-19 86.3
MS.gene47577.t1 MTR_8g068740 27.437 277 152 7 26 293 41 277 2.74e-17 81.3
MS.gene47577.t1 MTR_3g096050 29.876 241 154 9 32 266 31 262 6.57e-17 80.1
MS.gene47577.t1 MTR_1g036510 29.907 214 136 7 56 263 80 285 8.91e-16 77.4
MS.gene47577.t1 MTR_8g068600 26.556 241 150 9 38 264 27 254 7.76e-15 74.3
MS.gene47577.t1 MTR_7g012020 29.630 216 143 6 26 236 33 244 1.01e-14 73.6
MS.gene47577.t1 MTR_4g025570 45.783 83 38 3 6 88 16 91 1.63e-14 72.4
MS.gene47577.t1 MTR_4g025570 38.542 96 55 3 265 359 122 214 1.49e-12 66.6
MS.gene47577.t1 MTR_8g068620 36.170 94 60 0 173 266 20 113 2.00e-13 66.6
MS.gene47577.t1 MTR_8g068760 42.529 87 46 2 263 348 135 218 9.26e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47577.t1 AT4G35150 33.520 358 178 11 16 362 15 323 1.27e-50 172
MS.gene47577.t1 AT4G35160 34.435 363 211 11 16 362 29 380 6.10e-47 164
MS.gene47577.t1 AT5G54160 34.006 347 198 9 28 361 31 359 6.35e-45 158
MS.gene47577.t1 AT3G53140 32.286 350 208 13 26 360 21 356 7.95e-40 144
MS.gene47577.t1 AT1G77520 31.551 374 223 13 9 362 20 380 5.38e-39 143
MS.gene47577.t1 AT1G51990 31.267 371 230 13 4 363 6 362 1.71e-38 141
MS.gene47577.t1 AT1G51990 30.343 379 223 12 4 363 6 362 2.03e-38 141
MS.gene47577.t1 AT5G53810 31.624 351 212 12 29 362 38 377 2.92e-34 130
MS.gene47577.t1 AT1G77530 33.333 369 223 13 9 362 20 380 3.93e-34 130
MS.gene47577.t1 AT1G33030 30.702 342 220 10 28 362 18 349 6.02e-34 129
MS.gene47577.t1 AT1G63140 29.301 372 235 12 9 362 19 380 2.26e-32 125
MS.gene47577.t1 AT1G63140 29.301 372 235 12 9 362 39 400 2.32e-32 125
MS.gene47577.t1 AT1G62900 35.681 213 118 6 153 362 8 204 2.36e-25 102
MS.gene47577.t1 AT1G76790 27.933 358 218 12 7 344 9 346 3.89e-25 105
MS.gene47577.t1 AT5G37170 30.928 194 128 4 172 362 43 233 2.54e-23 97.4
MS.gene47577.t1 AT5G37170 30.928 194 128 4 172 362 143 333 6.26e-23 98.2
MS.gene47577.t1 AT5G37170 31.667 180 114 5 187 360 7 183 3.83e-22 92.8
MS.gene47577.t1 AT1G21130 27.089 347 200 15 31 350 40 360 2.99e-21 94.4
MS.gene47577.t1 AT1G21110 26.023 342 200 14 31 345 40 355 2.50e-20 91.7
MS.gene47577.t1 AT1G21100 26.163 344 207 13 31 350 40 360 2.64e-20 91.7
MS.gene47577.t1 AT1G21120 25.581 344 209 12 31 350 40 360 6.53e-20 90.5
MS.gene47577.t1 AT1G21120 25.581 344 209 12 31 350 88 408 8.38e-20 90.5
MS.gene47577.t1 AT1G63140 27.306 271 174 9 9 263 39 302 9.03e-15 74.7
MS.gene47577.t1 AT1G33030 28.099 242 161 8 28 263 18 252 6.79e-13 68.6
MS.gene47577.t1 AT1G33030 28.033 239 159 8 31 263 21 252 1.13e-12 68.2

Find 65 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCAACAGGGGAGAGCTTC+TGG 0.191292 4.3:-25263285 MS.gene47577:CDS
GCTGTTCTTCAATATCTTTA+AGG 0.195840 4.3:+25262607 None:intergenic
TTTGAAACTTCATCCTTCTA+AGG 0.260291 4.3:-25263560 MS.gene47577:CDS
CGAACACGTGTTTGTTCTCT+TGG 0.272286 4.3:+25263173 None:intergenic
TGTTTATCTTCAATTGTTAA+AGG 0.290462 4.3:-25263391 MS.gene47577:CDS
CAGGTTGTTGGTAACTTAAC+TGG 0.291153 4.3:-25263046 MS.gene47577:CDS
TACAGTGGGTTCTGCATGAT+TGG 0.326455 4.3:-25262643 MS.gene47577:intron
AGAAACCGTTGTGTGTTAAA+AGG 0.336053 4.3:+25263512 None:intergenic
TGTATACATGTTTGTACAAA+TGG 0.340652 4.3:+25263686 None:intergenic
CAAACACGTGTTCGAGGGTT+TGG 0.341667 4.3:-25263164 MS.gene47577:CDS
ACACTGTTTGAGTGTGCAAC+AGG 0.344866 4.3:-25263298 MS.gene47577:CDS
GTGATGCTTACAATGTTTCT+TGG 0.364082 4.3:-25262470 MS.gene47577:CDS
TTCATTGAACCATTTCTTTG+AGG 0.375008 4.3:+25263333 None:intergenic
TGGATTAACTGAACTGCAAT+TGG 0.377228 4.3:-25262504 MS.gene47577:CDS
AATGAAAATTTGAACTTTGT+TGG 0.379769 4.3:-25263022 MS.gene47577:CDS
GCTAATTCAGAAAGAGTCAT+TGG 0.394069 4.3:+25263589 None:intergenic
CCACTTATGAGAAGCTTTGA+AGG 0.396791 4.3:+25263424 None:intergenic
GTTGATGTTGGAGGTGGTAC+TGG 0.401447 4.3:-25263133 MS.gene47577:CDS
TTGGAGTCTCTTGTTGATGT+TGG 0.404723 4.3:-25263145 MS.gene47577:CDS
CTCCACATGTCTAAAGAACT+TGG 0.410016 4.3:+25263358 None:intergenic
TGAAGTATATTAACCTTAGA+AGG 0.411294 4.3:+25263547 None:intergenic
AAGAAAGAACAAAGCAAGAA+TGG 0.426132 4.3:-25262445 MS.gene47577:CDS
ACTGCAATTGGACTATGATT+TGG 0.427028 4.3:-25262492 MS.gene47577:CDS
GAGAGACTTGAAGCCAGAAA+TGG 0.427559 4.3:+25262376 None:intergenic
CCTTCAAAGCTTCTCATAAG+TGG 0.434600 4.3:-25263424 MS.gene47577:CDS
AGAGACTTGAAGCCAGAAAT+GGG 0.439445 4.3:+25262377 None:intergenic
TGTGGAGTTCCTCAAAGAAA+TGG 0.466565 4.3:-25263342 MS.gene47577:CDS
TGCAACAGGGGAGAGCTTCT+GGG 0.468202 4.3:-25263284 MS.gene47577:CDS
GTGTTAAAAGGCGCAAGAAT+CGG 0.481292 4.3:+25263524 None:intergenic
GAAAATTTGAACTTTGTTGG+TGG 0.485249 4.3:-25263019 MS.gene47577:CDS
TTCACTTTCTTCAGAACCAT+TGG 0.487182 4.3:+25263726 None:intergenic
TTTGATCAACCACAGGTTGT+TGG 0.492105 4.3:-25263058 MS.gene47577:CDS
CTCAAATCAGCCATGGAACT+AGG 0.496080 4.3:-25263643 MS.gene47577:CDS
GAGTCTCTTGTTGATGTTGG+AGG 0.496314 4.3:-25263142 MS.gene47577:CDS
ATCCAAGTTCTTTAGACATG+TGG 0.496638 4.3:-25263360 MS.gene47577:CDS
GAGTATGTTTCAAGATGCTA+TGG 0.498973 4.3:-25263227 MS.gene47577:CDS
ACAATAGTGAAAGGCAAAGA+AGG 0.501253 4.3:-25263481 MS.gene47577:CDS
ATCAGCTATGCCTAGTTCCA+TGG 0.501316 4.3:+25263633 None:intergenic
GAAATGGTTCAATGAAGATA+AGG 0.502299 4.3:-25263326 MS.gene47577:CDS
AGAAAGAACAAAGCAAGAAT+GGG 0.507206 4.3:-25262444 MS.gene47577:CDS
ACAATTGAAGATAAACATGT+TGG 0.521606 4.3:+25263397 None:intergenic
ATACAACTTTGTTAGTTCCA+TGG 0.535416 4.3:-25263668 MS.gene47577:CDS
GAAATGGCTTTCACTACCAA+TGG 0.538698 4.3:-25263742 MS.gene47577:CDS
AGAGAACAAACACGTGTTCG+AGG 0.543577 4.3:-25263170 MS.gene47577:CDS
ATTTCACACAAAGGAAAGGA+TGG 0.547784 4.3:-25262575 MS.gene47577:CDS
AGTAAAACAGCATCAGCAGA+AGG 0.551999 4.3:+25262977 None:intergenic
TCTCTTGTTGATGTTGGAGG+TGG 0.554073 4.3:-25263139 MS.gene47577:CDS
AGGATGAAGTTTCAAAGAAG+AGG 0.557817 4.3:+25263567 None:intergenic
CATGGCTGATTTGAGTGCCA+TGG 0.563511 4.3:+25263651 None:intergenic
AGCAATTTCACACAAAGGAA+AGG 0.577136 4.3:-25262579 MS.gene47577:CDS
GAGAACAAACACGTGTTCGA+GGG 0.583061 4.3:-25263169 MS.gene47577:CDS
CACTGTTTGAGTGTGCAACA+GGG 0.603315 4.3:-25263297 MS.gene47577:CDS
CATGGCACTCAAATCAGCCA+TGG 0.607444 4.3:-25263650 MS.gene47577:CDS
GATGTTGGAGGTGGTACTGG+TGG 0.610342 4.3:-25263130 MS.gene47577:CDS
GCTGATGCAATCCATAACCA+TGG 0.611918 4.3:-25263616 MS.gene47577:CDS
TGTACAAATGGATTTGAGCA+TGG 0.612036 4.3:+25263698 None:intergenic
AAAACAGTGCATTTAAGGTG+AGG 0.613644 4.3:+25263079 None:intergenic
GTAAAACAGCATCAGCAGAA+GGG 0.623492 4.3:+25262978 None:intergenic
AAAGAAGCAATTTCACACAA+AGG 0.626707 4.3:-25262584 MS.gene47577:CDS
GAGACTTGAAGCCAGAAATG+GGG 0.636393 4.3:+25262378 None:intergenic
AGACTTGAAGCCAGAAATGG+GGG 0.646680 4.3:+25262379 None:intergenic
ACTGTTTGAGTGTGCAACAG+GGG 0.649035 4.3:-25263296 MS.gene47577:CDS
GATGAAACAAGTGACGATCG+TGG 0.671140 4.3:-25262524 MS.gene47577:CDS
CTTATGAGAAGCTTTGAAGG+AGG 0.681784 4.3:+25263427 None:intergenic
GTTAAGTTACCAACAACCTG+TGG 0.693381 4.3:+25263049 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATAACTTTTACTAATA+AGG - chr4.3:25263347-25263366 MS.gene47577:CDS 10.0%
!!! TGACATTTAATTTAGTTATT+TGG + chr4.3:25263422-25263441 None:intergenic 15.0%
!!! TTTTACTAATAAGGATAAAA+TGG - chr4.3:25263356-25263375 MS.gene47577:CDS 15.0%
!! AAGTTATATAACAAGATTGA+AGG + chr4.3:25263338-25263357 None:intergenic 20.0%
!! AATGAAAATTTGAACTTTGT+TGG - chr4.3:25263075-25263094 MS.gene47577:CDS 20.0%
!! TGTTTATCTTCAATTGTTAA+AGG - chr4.3:25262706-25262725 MS.gene47577:intron 20.0%
!!! ACAAATTAAGATGCATATTT+TGG - chr4.3:25263391-25263410 MS.gene47577:CDS 20.0%
!!! GATTGTTACTATTTCAATTT+TGG - chr4.3:25263162-25263181 MS.gene47577:CDS 20.0%
! ACAATTGAAGATAAACATGT+TGG + chr4.3:25262703-25262722 None:intergenic 25.0%
! TGAAGTATATTAACCTTAGA+AGG + chr4.3:25262553-25262572 None:intergenic 25.0%
! TGTATACATGTTTGTACAAA+TGG + chr4.3:25262414-25262433 None:intergenic 25.0%
! TGTTAGACATTAAAATGTGT+AGG - chr4.3:25263304-25263323 MS.gene47577:CDS 25.0%
! TTTGCAAAAACAATAGTGAA+AGG - chr4.3:25262607-25262626 MS.gene47577:CDS 25.0%
!! CAATTGTCTTTTATTACAGT+GGG - chr4.3:25263440-25263459 MS.gene47577:CDS 25.0%
!! TCAATTGTCTTTTATTACAG+TGG - chr4.3:25263439-25263458 MS.gene47577:CDS 25.0%
AAAGAAGCAATTTCACACAA+AGG - chr4.3:25263513-25263532 MS.gene47577:CDS 30.0%
AAGAAAGAACAAAGCAAGAA+TGG - chr4.3:25263652-25263671 MS.gene47577:CDS 30.0%
AGAAAGAACAAAGCAAGAAT+GGG - chr4.3:25263653-25263672 MS.gene47577:CDS 30.0%
ATACAACTTTGTTAGTTCCA+TGG - chr4.3:25262429-25262448 MS.gene47577:CDS 30.0%
GAAAATTTGAACTTTGTTGG+TGG - chr4.3:25263078-25263097 MS.gene47577:CDS 30.0%
GAAATGGTTCAATGAAGATA+AGG - chr4.3:25262771-25262790 MS.gene47577:intron 30.0%
GATCAAAAACAGTGCATTTA+AGG + chr4.3:25263026-25263045 None:intergenic 30.0%
GCTGTTCTTCAATATCTTTA+AGG + chr4.3:25263493-25263512 None:intergenic 30.0%
TTCATTGAACCATTTCTTTG+AGG + chr4.3:25262767-25262786 None:intergenic 30.0%
! TCGTCGACTAAATTTAATTC+TGG + chr4.3:25263244-25263263 None:intergenic 30.0%
! TTTGAAACTTCATCCTTCTA+AGG - chr4.3:25262537-25262556 MS.gene47577:CDS 30.0%
ACAATAGTGAAAGGCAAAGA+AGG - chr4.3:25262616-25262635 MS.gene47577:CDS 35.0%
ACTGCAATTGGACTATGATT+TGG - chr4.3:25263605-25263624 MS.gene47577:CDS 35.0%
AGAAACCGTTGTGTGTTAAA+AGG + chr4.3:25262588-25262607 None:intergenic 35.0%
AGCAATTTCACACAAAGGAA+AGG - chr4.3:25263518-25263537 MS.gene47577:CDS 35.0%
AGGATGAAGTTTCAAAGAAG+AGG + chr4.3:25262533-25262552 None:intergenic 35.0%
ATCCAAGTTCTTTAGACATG+TGG - chr4.3:25262737-25262756 MS.gene47577:intron 35.0%
ATTTCACACAAAGGAAAGGA+TGG - chr4.3:25263522-25263541 MS.gene47577:CDS 35.0%
GAAAAACTCATATACGATGC+AGG - chr4.3:25263675-25263694 MS.gene47577:CDS 35.0%
GAGTATGTTTCAAGATGCTA+TGG - chr4.3:25262870-25262889 MS.gene47577:intron 35.0%
GCTAATTCAGAAAGAGTCAT+TGG + chr4.3:25262511-25262530 None:intergenic 35.0%
GTCATTGTCAATATTGTGTC+CGG + chr4.3:25263214-25263233 None:intergenic 35.0%
GTGATGCTTACAATGTTTCT+TGG - chr4.3:25263627-25263646 MS.gene47577:CDS 35.0%
TACAAAATTACCCCCATTTC+TGG - chr4.3:25263708-25263727 MS.gene47577:CDS 35.0%
TGGATTAACTGAACTGCAAT+TGG - chr4.3:25263593-25263612 MS.gene47577:CDS 35.0%
TTCACTTTCTTCAGAACCAT+TGG + chr4.3:25262374-25262393 None:intergenic 35.0%
! AAAACAGTGCATTTAAGGTG+AGG + chr4.3:25263021-25263040 None:intergenic 35.0%
! TGTACAAATGGATTTGAGCA+TGG + chr4.3:25262402-25262421 None:intergenic 35.0%
!! CAATTTTGGTCTATGAAGCA+CGG - chr4.3:25263176-25263195 MS.gene47577:CDS 35.0%
!!! AAAGAGTCATTGGTTTTCCA+TGG + chr4.3:25262501-25262520 None:intergenic 35.0%
!!! TCATTGGTTTTCCATGGTTA+TGG + chr4.3:25262495-25262514 None:intergenic 35.0%
!!! TGCTGATGCTGTTTTACTTA+AGG - chr4.3:25263125-25263144 MS.gene47577:CDS 35.0%
AGAGACTTGAAGCCAGAAAT+GGG + chr4.3:25263723-25263742 None:intergenic 40.0%
AGTAAAACAGCATCAGCAGA+AGG + chr4.3:25263123-25263142 None:intergenic 40.0%
CCTTCAAAGCTTCTCATAAG+TGG - chr4.3:25262673-25262692 MS.gene47577:intron 40.0%
CTCCACATGTCTAAAGAACT+TGG + chr4.3:25262742-25262761 None:intergenic 40.0%
GTAAAACAGCATCAGCAGAA+GGG + chr4.3:25263122-25263141 None:intergenic 40.0%
GTTAAGTTACCAACAACCTG+TGG + chr4.3:25263051-25263070 None:intergenic 40.0%
TGTGGAGTTCCTCAAAGAAA+TGG - chr4.3:25262755-25262774 MS.gene47577:intron 40.0%
TTGGAGTCTCTTGTTGATGT+TGG - chr4.3:25262952-25262971 MS.gene47577:intron 40.0%
TTTGATCAACCACAGGTTGT+TGG - chr4.3:25263039-25263058 MS.gene47577:CDS 40.0%
! CCACTTATGAGAAGCTTTGA+AGG + chr4.3:25262676-25262695 None:intergenic 40.0%
! GTGTTAAAAGGCGCAAGAAT+CGG + chr4.3:25262576-25262595 None:intergenic 40.0%
! TTGCGCCTTTTAACACACAA+CGG - chr4.3:25262580-25262599 MS.gene47577:CDS 40.0%
!! CAGGTTGTTGGTAACTTAAC+TGG - chr4.3:25263051-25263070 MS.gene47577:CDS 40.0%
!! CTTATGAGAAGCTTTGAAGG+AGG + chr4.3:25262673-25262692 None:intergenic 40.0%
!!! CACTGTTTTTGATCAACCAC+AGG - chr4.3:25263032-25263051 MS.gene47577:CDS 40.0%
ACACTGTTTGAGTGTGCAAC+AGG - chr4.3:25262799-25262818 MS.gene47577:intron 45.0%
ACTGTTTGAGTGTGCAACAG+GGG - chr4.3:25262801-25262820 MS.gene47577:intron 45.0%
AGACTTGAAGCCAGAAATGG+GGG + chr4.3:25263721-25263740 None:intergenic 45.0%
AGAGAACAAACACGTGTTCG+AGG - chr4.3:25262927-25262946 MS.gene47577:intron 45.0%
ATCAGCTATGCCTAGTTCCA+TGG + chr4.3:25262467-25262486 None:intergenic 45.0%
CACTGTTTGAGTGTGCAACA+GGG - chr4.3:25262800-25262819 MS.gene47577:intron 45.0%
CGAACACGTGTTTGTTCTCT+TGG + chr4.3:25262927-25262946 None:intergenic 45.0%
CTCAAATCAGCCATGGAACT+AGG - chr4.3:25262454-25262473 MS.gene47577:CDS 45.0%
GAGAACAAACACGTGTTCGA+GGG - chr4.3:25262928-25262947 MS.gene47577:intron 45.0%
GAGACTTGAAGCCAGAAATG+GGG + chr4.3:25263722-25263741 None:intergenic 45.0%
GAGAGACTTGAAGCCAGAAA+TGG + chr4.3:25263724-25263743 None:intergenic 45.0%
GAGTCTCTTGTTGATGTTGG+AGG - chr4.3:25262955-25262974 MS.gene47577:intron 45.0%
GATGAAACAAGTGACGATCG+TGG - chr4.3:25263573-25263592 MS.gene47577:CDS 45.0%
GCTGATGCAATCCATAACCA+TGG - chr4.3:25262481-25262500 MS.gene47577:CDS 45.0%
TCTCTTGTTGATGTTGGAGG+TGG - chr4.3:25262958-25262977 MS.gene47577:intron 45.0%
! TACAGTGGGTTCTGCATGAT+TGG - chr4.3:25263454-25263473 MS.gene47577:CDS 45.0%
CAAACACGTGTTCGAGGGTT+TGG - chr4.3:25262933-25262952 MS.gene47577:intron 50.0%
CATGGCACTCAAATCAGCCA+TGG - chr4.3:25262447-25262466 MS.gene47577:CDS 50.0%
GTTGATGTTGGAGGTGGTAC+TGG - chr4.3:25262964-25262983 MS.gene47577:intron 50.0%
!! CATGGCTGATTTGAGTGCCA+TGG + chr4.3:25262449-25262468 None:intergenic 50.0%
TGCAACAGGGGAGAGCTTCT+GGG - chr4.3:25262813-25262832 MS.gene47577:intron 55.0%
! GATGTTGGAGGTGGTACTGG+TGG - chr4.3:25262967-25262986 MS.gene47577:intron 55.0%
AGCACGGACACTGCACACAC+CGG - chr4.3:25263192-25263211 MS.gene47577:CDS 60.0%
GTGCAACAGGGGAGAGCTTC+TGG - chr4.3:25262812-25262831 MS.gene47577:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 25262358 25263761 25262358 ID=MS.gene47577
chr4.3 mRNA 25262358 25263761 25262358 ID=MS.gene47577.t1;Parent=MS.gene47577
chr4.3 exon 25262973 25263761 25262973 ID=MS.gene47577.t1.exon1;Parent=MS.gene47577.t1
chr4.3 CDS 25262973 25263761 25262973 ID=cds.MS.gene47577.t1;Parent=MS.gene47577.t1
chr4.3 exon 25262358 25262660 25262358 ID=MS.gene47577.t1.exon2;Parent=MS.gene47577.t1
chr4.3 CDS 25262358 25262660 25262358 ID=cds.MS.gene47577.t1;Parent=MS.gene47577.t1
Gene Sequence

>MS.gene47577

ATGGCTTTCACTACCAATGGTTCTGAAGAAAGTGAACTGTACCATGCTCAAATCCATTTGTACAAACATGTATACAACTTTGTTAGTTCCATGGCACTCAAATCAGCCATGGAACTAGGCATAGCTGATGCAATCCATAACCATGGAAAACCAATGACTCTTTCTGAATTAGCCTCTTCTTTGAAACTTCATCCTTCTAAGGTTAATATACTTCACCGATTCTTGCGCCTTTTAACACACAACGGTTTCTTTGCAAAAACAATAGTGAAAGGCAAAGAAGGAGAAGAAGAAGAAATAGCATATTCTCTCACACCTCCTTCAAAGCTTCTCATAAGTGGAAAACCAACATGTTTATCTTCAATTGTTAAAGGAGCACTTCATCCAAGTTCTTTAGACATGTGGAGTTCCTCAAAGAAATGGTTCAATGAAGATAAGGAACAAACACTGTTTGAGTGTGCAACAGGGGAGAGCTTCTGGGATTTTCTTAACAAAGATTCTGAATCTAGTACTTTGAGTATGTTTCAAGATGCTATGGCATCTGATTCTAGAATGTTTAAGCTTGTTCTCCAAGAGAACAAACACGTGTTCGAGGGTTTGGAGTCTCTTGTTGATGTTGGAGGTGGTACTGGTGGTGTCACAAAACTCATTAATGAAATTTTTCCTCACCTTAAATGCACTGTTTTTGATCAACCACAGGTTGTTGGTAACTTAACTGGAAATGAAAATTTGAACTTTGTTGGTGGAGATATGTTCAAATCTATCCCTTCTGCTGATGCTGTTTTACTTAAGGTATGCATGTTCAACGATTGTTACTATTTCAATTTTGGTCTATGAAGCACGGACACTGCACACACCGGACACAATATTGACAATGACATAGACACCAGAATTAAATTTAGTCGACGAAACTGTTTGAATTTAATAACATATGACTGCGTCGAGATAATGTTAGACATTAAAATGTGTAGGACACATTACCTTCAATCTTGTTATATAACTTTTACTAATAAGGATAAAATGGTAGTAACTAGTAACAAATTAAGATGCATATTTTGGATCAACCAAATAACTAAATTAAATGTCAATTGTCTTTTATTACAGTGGGTTCTGCATGATTGGAATGATGAACAATCCTTAAAGATATTGAAGAACAGCAAAGAAGCAATTTCACACAAAGGAAAGGATGGAAAAGTGATTATCATCGATATATCAATTGATGAAACAAGTGACGATCGTGGATTAACTGAACTGCAATTGGACTATGATTTGGTGATGCTTACAATGTTTCTTGGTAAAGAAAGAACAAAGCAAGAATGGGAAAAACTCATATACGATGCAGGTTTTAGCAGCTACAAAATTACCCCCATTTCTGGCTTCAAGTCTCTCATTGAAGTTTATCCTTAA
Protein sequence

>MS.gene47577.t1

MAFTTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLSELASSLKLHPSKVNILHRFLRLLTHNGFFAKTIVKGKEGEEEEIAYSLTPPSKLLISGKPTCLSSIVKGALHPSSLDMWSSSKKWFNEDKEQTLFECATGESFWDFLNKDSESSTLSMFQDAMASDSRMFKLVLQENKHVFEGLESLVDVGGGTGGVTKLINEIFPHLKCTVFDQPQVVGNLTGNENLNFVGGDMFKSIPSADAVLLKWVLHDWNDEQSLKILKNSKEAISHKGKDGKVIIIDISIDETSDDRGLTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFSSYKITPISGFKSLIEVYP