Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058179.t1 | XP_003594829.1 | 99.4 | 157 | 1 | 0 | 1 | 157 | 1 | 157 | 1.60E-81 | 312 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058179.t1 | P93333 | 99.4 | 157 | 1 | 0 | 1 | 157 | 1 | 157 | 3.8e-84 | 312.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058179.t1 | A0A396J749 | 99.4 | 157 | 1 | 0 | 1 | 157 | 1 | 157 | 1.1e-81 | 312.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050380 | MS.gene058179 | 0.845475 | 3.97E-59 | -1.69E-46 |
| MS.gene050381 | MS.gene058179 | 0.832973 | 6.96E-56 | -1.69E-46 |
| MS.gene050809 | MS.gene058179 | 0.818221 | 2.19E-52 | -1.69E-46 |
| MS.gene05104 | MS.gene058179 | 0.818823 | 1.60E-52 | -1.69E-46 |
| MS.gene051632 | MS.gene058179 | 0.822851 | 1.90E-53 | -1.69E-46 |
| MS.gene053488 | MS.gene058179 | 0.809305 | 2.02E-50 | -1.69E-46 |
| MS.gene055124 | MS.gene058179 | 0.850964 | 1.21E-60 | -1.69E-46 |
| MS.gene055959 | MS.gene058179 | 0.857877 | 1.21E-62 | -1.69E-46 |
| MS.gene056313 | MS.gene058179 | 0.824469 | 7.94E-54 | -1.69E-46 |
| MS.gene058093 | MS.gene058179 | 0.870393 | 1.51E-66 | -1.69E-46 |
| MS.gene058099 | MS.gene058179 | 0.84178 | 3.86E-58 | -1.69E-46 |
| MS.gene058108 | MS.gene058179 | 0.870667 | 1.23E-66 | -1.69E-46 |
| MS.gene058109 | MS.gene058179 | 0.838983 | 2.08E-57 | -1.69E-46 |
| MS.gene058170 | MS.gene058179 | 0.81363 | 2.32E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene058179 | 0.862208 | 5.96E-64 | -1.69E-46 |
| MS.gene058173 | MS.gene058179 | 0.862654 | 4.35E-64 | -1.69E-46 |
| MS.gene058174 | MS.gene058179 | 0.8154 | 9.43E-52 | -1.69E-46 |
| MS.gene058175 | MS.gene058179 | 0.867087 | 1.77E-65 | -1.69E-46 |
| MS.gene058176 | MS.gene058179 | 0.863203 | 2.94E-64 | -1.69E-46 |
| MS.gene058177 | MS.gene058179 | 0.802959 | 4.38E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene058180 | 0.916979 | 9.27E-86 | -1.69E-46 |
| MS.gene058179 | MS.gene058840 | 0.821366 | 4.19E-53 | -1.69E-46 |
| MS.gene058179 | MS.gene059037 | 0.905348 | 4.72E-80 | -1.69E-46 |
| MS.gene058179 | MS.gene059038 | 0.875531 | 2.86E-68 | -1.69E-46 |
| MS.gene058179 | MS.gene059044 | 0.858816 | 6.35E-63 | -1.69E-46 |
| MS.gene058179 | MS.gene059045 | 0.838405 | 2.93E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene059944 | 0.800927 | 1.15E-48 | -1.69E-46 |
| MS.gene058179 | MS.gene060205 | 0.835419 | 1.70E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene060578 | 0.836712 | 7.97E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene061402 | -0.832754 | 7.89E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene063073 | 0.812542 | 4.02E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene068296 | 0.890586 | 8.61E-74 | -1.69E-46 |
| MS.gene058179 | MS.gene068297 | 0.916846 | 1.09E-85 | -1.69E-46 |
| MS.gene058179 | MS.gene069431 | 0.841833 | 3.74E-58 | -1.69E-46 |
| MS.gene058179 | MS.gene071837 | 0.814512 | 1.48E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene071838 | 0.953073 | 6.10E-111 | -1.69E-46 |
| MS.gene058179 | MS.gene071839 | 0.824778 | 6.71E-54 | -1.69E-46 |
| MS.gene058179 | MS.gene071840 | 0.803463 | 3.44E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene071842 | 0.820051 | 8.41E-53 | -1.69E-46 |
| MS.gene058179 | MS.gene071843 | 0.866143 | 3.54E-65 | -1.69E-46 |
| MS.gene058179 | MS.gene071844 | 0.811032 | 8.58E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene071942 | 0.838972 | 2.09E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene072609 | 0.832666 | 8.30E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene072806 | 0.828153 | 1.05E-54 | -1.69E-46 |
| MS.gene058179 | MS.gene07591 | 0.820284 | 7.44E-53 | -1.69E-46 |
| MS.gene058179 | MS.gene08407 | 0.810733 | 9.95E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene21350 | 0.817935 | 2.55E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene21577 | 0.819965 | 8.80E-53 | -1.69E-46 |
| MS.gene058179 | MS.gene21757 | 0.843591 | 1.28E-58 | -1.69E-46 |
| MS.gene058179 | MS.gene21758 | 0.845004 | 5.32E-59 | -1.69E-46 |
| MS.gene058179 | MS.gene21768 | 0.828478 | 8.79E-55 | -1.69E-46 |
| MS.gene058179 | MS.gene21772 | 0.84663 | 1.92E-59 | -1.69E-46 |
| MS.gene058179 | MS.gene21851 | 0.838022 | 3.68E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene21856 | 0.836758 | 7.76E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene21867 | 0.863877 | 1.82E-64 | -1.69E-46 |
| MS.gene058179 | MS.gene21868 | 0.834262 | 3.32E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene24540 | 0.806795 | 6.92E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene24974 | 0.915733 | 4.14E-85 | -1.69E-46 |
| MS.gene058179 | MS.gene26243 | 0.827324 | 1.67E-54 | -1.69E-46 |
| MS.gene058179 | MS.gene26403 | 0.846144 | 2.61E-59 | -1.69E-46 |
| MS.gene058179 | MS.gene26517 | 0.815616 | 8.44E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene27550 | 0.838801 | 2.32E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene27779 | 0.844159 | 8.99E-59 | -1.69E-46 |
| MS.gene058179 | MS.gene27827 | 0.825624 | 4.24E-54 | -1.69E-46 |
| MS.gene058179 | MS.gene28724 | 0.811312 | 7.46E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene32972 | 0.806139 | 9.51E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene33428 | 0.871521 | 6.42E-67 | -1.69E-46 |
| MS.gene058179 | MS.gene33429 | 0.892036 | 2.30E-74 | -1.69E-46 |
| MS.gene058179 | MS.gene33682 | 0.877657 | 5.27E-69 | -1.69E-46 |
| MS.gene058179 | MS.gene33683 | 0.888661 | 4.84E-73 | -1.69E-46 |
| MS.gene058179 | MS.gene35105 | 0.822045 | 2.92E-53 | -1.69E-46 |
| MS.gene058179 | MS.gene35208 | 0.83885 | 2.25E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene38094 | 0.806024 | 1.01E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene38095 | 0.812779 | 3.57E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene38097 | 0.84557 | 3.74E-59 | -1.69E-46 |
| MS.gene058179 | MS.gene38155 | 0.824902 | 6.28E-54 | -1.69E-46 |
| MS.gene058179 | MS.gene38172 | 0.813983 | 1.94E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene38173 | 0.833301 | 5.77E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene38706 | 0.80338 | 3.58E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene39533 | 0.833596 | 4.87E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene39535 | 0.826231 | 3.04E-54 | -1.69E-46 |
| MS.gene058179 | MS.gene39635 | 0.815661 | 8.24E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene40171 | 0.812937 | 3.30E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene40480 | 0.81393 | 1.99E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene41015 | 0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene44721 | 0.818264 | 2.15E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene44722 | 0.83159 | 1.53E-55 | -1.69E-46 |
| MS.gene058179 | MS.gene46024 | 0.86145 | 1.02E-63 | -1.69E-46 |
| MS.gene058179 | MS.gene46025 | 0.875711 | 2.48E-68 | -1.69E-46 |
| MS.gene058179 | MS.gene46027 | 0.842307 | 2.80E-58 | -1.69E-46 |
| MS.gene058179 | MS.gene46434 | 0.833516 | 5.10E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene46779 | 0.809309 | 2.02E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene47576 | 0.835964 | 1.23E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene47577 | 0.805126 | 1.55E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene48182 | 0.802033 | 6.80E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene48183 | 0.838574 | 2.65E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene48184 | 0.818774 | 1.64E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene48314 | 0.835521 | 1.60E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene50789 | 0.805001 | 1.65E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene51118 | 0.826504 | 2.62E-54 | -1.69E-46 |
| MS.gene058179 | MS.gene51120 | 0.814691 | 1.35E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene51523 | 0.801726 | 7.86E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene51620 | 0.807634 | 4.59E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene52700 | 0.861238 | 1.18E-63 | -1.69E-46 |
| MS.gene058179 | MS.gene56009 | 0.832819 | 7.60E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene59001 | 0.84182 | 3.77E-58 | -1.69E-46 |
| MS.gene058179 | MS.gene59005 | 0.874467 | 6.60E-68 | -1.69E-46 |
| MS.gene058179 | MS.gene59006 | 0.887914 | 9.38E-73 | -1.69E-46 |
| MS.gene058179 | MS.gene59273 | 0.815685 | 8.14E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene59394 | 0.857245 | 1.86E-62 | -1.69E-46 |
| MS.gene058179 | MS.gene59395 | 0.881832 | 1.73E-70 | -1.69E-46 |
| MS.gene058179 | MS.gene59396 | 0.86997 | 2.08E-66 | -1.69E-46 |
| MS.gene058179 | MS.gene60477 | 0.852714 | 3.85E-61 | -1.69E-46 |
| MS.gene058179 | MS.gene61194 | 0.876257 | 1.61E-68 | -1.69E-46 |
| MS.gene058179 | MS.gene62114 | 0.889243 | 2.88E-73 | -1.69E-46 |
| MS.gene058179 | MS.gene62120 | 0.854177 | 1.46E-61 | -1.69E-46 |
| MS.gene058179 | MS.gene63731 | 0.833362 | 5.57E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene64372 | 0.832378 | 9.78E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene64686 | 0.872229 | 3.73E-67 | -1.69E-46 |
| MS.gene058179 | MS.gene64687 | 0.854559 | 1.14E-61 | -1.69E-46 |
| MS.gene058179 | MS.gene65287 | 0.818837 | 1.59E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene65865 | 0.804375 | 2.23E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene66895 | 0.839614 | 1.43E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene68121 | 0.819473 | 1.14E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene70061 | 0.805451 | 1.33E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene70062 | 0.820902 | 5.37E-53 | -1.69E-46 |
| MS.gene058179 | MS.gene70420 | 0.864851 | 9.03E-65 | -1.69E-46 |
| MS.gene058179 | MS.gene70474 | 0.812907 | 3.35E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene71525 | 0.861254 | 1.17E-63 | -1.69E-46 |
| MS.gene058179 | MS.gene75392 | 0.867987 | 9.12E-66 | -1.69E-46 |
| MS.gene058179 | MS.gene75396 | 0.905329 | 4.82E-80 | -1.69E-46 |
| MS.gene058179 | MS.gene77223 | 0.820916 | 5.33E-53 | -1.69E-46 |
| MS.gene058179 | MS.gene77471 | 0.864395 | 1.25E-64 | -1.69E-46 |
| MS.gene058179 | MS.gene78687 | 0.812957 | 3.26E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene80092 | 0.819196 | 1.32E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene81770 | 0.808851 | 2.53E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene84374 | 0.81511 | 1.09E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene84568 | 0.87031 | 1.61E-66 | -1.69E-46 |
| MS.gene058179 | MS.gene85638 | 0.815245 | 1.02E-51 | -1.69E-46 |
| MS.gene058179 | MS.gene91973 | 0.847799 | 9.20E-60 | -1.69E-46 |
| MS.gene058179 | MS.gene91976 | 0.83985 | 1.24E-57 | -1.69E-46 |
| MS.gene058179 | MS.gene91977 | 0.848384 | 6.34E-60 | -1.69E-46 |
| MS.gene058179 | MS.gene92296 | 0.809558 | 1.78E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene92808 | 0.822818 | 1.93E-53 | -1.69E-46 |
| MS.gene058179 | MS.gene94790 | 0.873013 | 2.04E-67 | -1.69E-46 |
| MS.gene058179 | MS.gene96173 | 0.861779 | 8.07E-64 | -1.69E-46 |
| MS.gene058179 | MS.gene96584 | 0.80854 | 2.95E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene97182 | 0.806093 | 9.72E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene97499 | 0.883807 | 3.28E-71 | -1.69E-46 |
| MS.gene058179 | MS.gene97500 | 0.90493 | 7.33E-80 | -1.69E-46 |
| MS.gene058179 | MS.gene98878 | 0.810235 | 1.28E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene98883 | 0.807966 | 3.91E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058179.t1 | MTR_2g035100 | 99.363 | 157 | 1 | 0 | 1 | 157 | 1 | 157 | 2.78e-109 | 308 |
| MS.gene058179.t1 | MTR_2g035105 | 98.089 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 6.21e-108 | 304 |
| MS.gene058179.t1 | MTR_2g035130 | 88.535 | 157 | 18 | 0 | 1 | 157 | 1 | 157 | 3.77e-97 | 277 |
| MS.gene058179.t1 | MTR_2g035150 | 84.713 | 157 | 24 | 0 | 1 | 157 | 1 | 157 | 2.16e-93 | 268 |
| MS.gene058179.t1 | MTR_2g035120 | 83.439 | 157 | 26 | 0 | 1 | 157 | 1 | 157 | 3.39e-93 | 267 |
| MS.gene058179.t1 | MTR_6g033450 | 71.975 | 157 | 44 | 0 | 1 | 157 | 1 | 157 | 2.05e-79 | 232 |
| MS.gene058179.t1 | MTR_4g120970 | 71.975 | 157 | 44 | 0 | 1 | 157 | 1 | 157 | 2.05e-79 | 232 |
| MS.gene058179.t1 | MTR_2g035320 | 65.605 | 157 | 54 | 0 | 1 | 157 | 1 | 157 | 2.70e-69 | 206 |
| MS.gene058179.t1 | MTR_2g035190 | 61.783 | 157 | 60 | 0 | 1 | 157 | 1 | 157 | 5.95e-66 | 198 |
| MS.gene058179.t1 | MTR_2g035210 | 61.146 | 157 | 61 | 0 | 1 | 157 | 1 | 157 | 9.49e-65 | 195 |
| MS.gene058179.t1 | MTR_2g035220 | 61.783 | 157 | 60 | 0 | 1 | 157 | 1 | 157 | 9.35e-64 | 192 |
| MS.gene058179.t1 | MTR_2g035170 | 59.873 | 157 | 63 | 0 | 1 | 157 | 1 | 157 | 4.41e-62 | 188 |
| MS.gene058179.t1 | MTR_2g435310 | 40.385 | 156 | 92 | 1 | 1 | 155 | 1 | 156 | 7.63e-39 | 129 |
| MS.gene058179.t1 | MTR_2g035320 | 63.542 | 96 | 35 | 0 | 62 | 157 | 27 | 122 | 4.89e-37 | 124 |
| MS.gene058179.t1 | MTR_4g120760 | 39.744 | 156 | 93 | 1 | 1 | 155 | 1 | 156 | 1.87e-36 | 123 |
| MS.gene058179.t1 | MTR_4g120760 | 39.744 | 156 | 93 | 1 | 1 | 155 | 1 | 156 | 2.13e-36 | 123 |
| MS.gene058179.t1 | MTR_4g120950 | 44.720 | 161 | 82 | 5 | 1 | 157 | 1 | 158 | 2.56e-33 | 115 |
| MS.gene058179.t1 | MTR_4g120940 | 62.500 | 88 | 30 | 1 | 70 | 157 | 35 | 119 | 2.42e-30 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGAAAGTAAGTTTCTTGA+TGG | 0.287513 | 2.1:+57706900 | None:intergenic |
| AGCTATATGCTTTAGTAATC+AGG | 0.289844 | 2.1:+57705582 | None:intergenic |
| CCCTTGATTTCCTCTTCACT+TGG | 0.329042 | 2.1:+57705663 | None:intergenic |
| CTTCAACAATTTCAATACTT+TGG | 0.342783 | 2.1:+57706941 | None:intergenic |
| ATCAATAACCTTTGGGATAA+GGG | 0.353978 | 2.1:+57706969 | None:intergenic |
| GGATGGCATCAATAACCTTT+GGG | 0.355456 | 2.1:+57706962 | None:intergenic |
| GAGATCTTCTCCACAGTGTC+TGG | 0.364866 | 2.1:+57705771 | None:intergenic |
| TGGATGGCATCAATAACCTT+TGG | 0.369451 | 2.1:+57706961 | None:intergenic |
| AAGAAACTTACTTTCGTTGA+AGG | 0.370871 | 2.1:-57706895 | MS.gene058179:intron |
| GGCTCTTGAAGGTTACGTTT+TGG | 0.372797 | 2.1:-57705610 | MS.gene058179:CDS |
| CATCAATAACCTTTGGGATA+AGG | 0.374566 | 2.1:+57706968 | None:intergenic |
| CAAAGTATTGAAATTGTTGA+AGG | 0.421501 | 2.1:-57706940 | MS.gene058179:CDS |
| GCTTACAACTACAGCATAGT+TGG | 0.432371 | 2.1:-57705807 | MS.gene058179:CDS |
| CATGGGTGTCTTCAACTTTG+AGG | 0.439280 | 2.1:-57707055 | MS.gene058179:CDS |
| AACAATTTCAATACTTTGGA+TGG | 0.439896 | 2.1:+57706945 | None:intergenic |
| ATTGAAATTGTTGAAGGAAA+CGG | 0.445615 | 2.1:-57706934 | MS.gene058179:CDS |
| AAATCAAGGGTGGCAAAGCT+AGG | 0.448601 | 2.1:-57705650 | MS.gene058179:CDS |
| TTTGAGTCTAAATTGTCTGC+AGG | 0.453113 | 2.1:-57705747 | MS.gene058179:CDS |
| TTTGCAATGGATCCTCCATC+TGG | 0.453770 | 2.1:+57705723 | None:intergenic |
| TACAGCATAGTTGGTGGTGG+TGG | 0.475041 | 2.1:-57705798 | MS.gene058179:CDS |
| AGAGCTTTGTAAAGTCTAGC+AGG | 0.480944 | 2.1:+57707009 | None:intergenic |
| GGTGGCAAAGCTAGGGGTGA+TGG | 0.489081 | 2.1:-57705642 | MS.gene058179:CDS |
| AATCAAGGGTGGCAAAGCTA+GGG | 0.491277 | 2.1:-57705649 | MS.gene058179:CDS |
| ACTGTGAAATACTTCACAAA+AGG | 0.498744 | 2.1:-57705696 | MS.gene058179:CDS |
| ACCAAGTGAAGAGGAAATCA+AGG | 0.498836 | 2.1:-57705664 | MS.gene058179:CDS |
| GTTGAAGGAAACGGTGGTGC+TGG | 0.507666 | 2.1:-57706925 | MS.gene058179:CDS |
| AAAGTATGATTTGCACAAAG+TGG | 0.527363 | 2.1:-57705853 | MS.gene058179:CDS |
| TTTCACAGTAAGCTTTGCAA+TGG | 0.527944 | 2.1:+57705710 | None:intergenic |
| TGCAGACAATTTAGACTCAA+AGG | 0.553148 | 2.1:+57705749 | None:intergenic |
| AAATTGTCTGCAGGGCCAGA+TGG | 0.558648 | 2.1:-57705738 | MS.gene058179:CDS |
| TTCTGATACCCTTATCCCAA+AGG | 0.569991 | 2.1:-57706977 | MS.gene058179:CDS |
| TTGAGTCTAAATTGTCTGCA+GGG | 0.579973 | 2.1:-57705746 | MS.gene058179:CDS |
| TGATGCTGCACCAAGTGAAG+AGG | 0.603594 | 2.1:-57705673 | MS.gene058179:CDS |
| TTGTCTGCAGGGCCAGATGG+AGG | 0.618761 | 2.1:-57705735 | MS.gene058179:CDS |
| TACAACTACAGCATAGTTGG+TGG | 0.619903 | 2.1:-57705804 | MS.gene058179:CDS |
| AACTACAGCATAGTTGGTGG+TGG | 0.623337 | 2.1:-57705801 | MS.gene058179:CDS |
| ATCAAGGGTGGCAAAGCTAG+GGG | 0.634094 | 2.1:-57705648 | MS.gene058179:CDS |
| AGCAGGAGCTACAATAGATG+TGG | 0.635518 | 2.1:+57707026 | None:intergenic |
| CCAAGTGAAGAGGAAATCAA+GGG | 0.664848 | 2.1:-57705663 | MS.gene058179:CDS |
| AGTGAAGAGGAAATCAAGGG+TGG | 0.695634 | 2.1:-57705660 | MS.gene058179:CDS |
| GAAATTGTTGAAGGAAACGG+TGG | 0.726108 | 2.1:-57706931 | MS.gene058179:CDS |
| TGGTGGACTTCCAGACACTG+TGG | 0.759361 | 2.1:-57705781 | MS.gene058179:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAATTTCATAATTAAG+AGG | + | chr2.1:57706008-57706027 | None:intergenic | 10.0% |
| !!! | TAAACATTTTATTATAGATA+GGG | + | chr2.1:57705830-57705849 | None:intergenic | 10.0% |
| !!! | TTAAACATTTTATTATAGAT+AGG | + | chr2.1:57705831-57705850 | None:intergenic | 10.0% |
| !!! | TGGATTTTTTTTTTTTTTGA+AGG | - | chr2.1:57706550-57706569 | MS.gene058179:intron | 15.0% |
| !!! | TTGAAAAACAGTTTTTAAAT+AGG | + | chr2.1:57706401-57706420 | None:intergenic | 15.0% |
| !!! | TTTTTGAAGGAATTATATAT+TGG | - | chr2.1:57706563-57706582 | MS.gene058179:intron | 15.0% |
| !! | AAACTACCAATATAAGTATT+TGG | + | chr2.1:57706167-57706186 | None:intergenic | 20.0% |
| !! | AAGATACATAACATAAATAG+TGG | - | chr2.1:57706233-57706252 | MS.gene058179:intron | 20.0% |
| !! | TAATATGTGAAAAATTGAAG+TGG | - | chr2.1:57705787-57705806 | MS.gene058179:CDS | 20.0% |
| !!! | AATTAACCGTCTTTATTTTA+AGG | + | chr2.1:57705893-57705912 | None:intergenic | 20.0% |
| !!! | AGTTTTTAAATAGGCTTATT+TGG | + | chr2.1:57706392-57706411 | None:intergenic | 20.0% |
| !!! | TGAGAAACAGTTTTTTTTTT+TGG | + | chr2.1:57706429-57706448 | None:intergenic | 20.0% |
| !!! | TTTTTACCGTAAAACAATAT+GGG | - | chr2.1:57706192-57706211 | MS.gene058179:intron | 20.0% |
| !!! | TTTTTTACCGTAAAACAATA+TGG | - | chr2.1:57706191-57706210 | MS.gene058179:intron | 20.0% |
| ! | AACAAGAATCAACAATTTGA+TGG | + | chr2.1:57706491-57706510 | None:intergenic | 25.0% |
| ! | AACAATTTCAATACTTTGGA+TGG | + | chr2.1:57705705-57705724 | None:intergenic | 25.0% |
| ! | AATAACCTAATTGAACTAAG+TGG | - | chr2.1:57706717-57706736 | MS.gene058179:intron | 25.0% |
| ! | AATAGGCTTATTTGGTATTA+TGG | + | chr2.1:57706384-57706403 | None:intergenic | 25.0% |
| ! | ATTGAAATTGTTGAAGGAAA+CGG | - | chr2.1:57705713-57705732 | MS.gene058179:CDS | 25.0% |
| ! | CCATATCCTTAAAATAAAGA+CGG | - | chr2.1:57705884-57705903 | MS.gene058179:intron | 25.0% |
| ! | CTTCAACAATTTCAATACTT+TGG | + | chr2.1:57705709-57705728 | None:intergenic | 25.0% |
| ! | TAGCTACCAAATACTTATAT+TGG | - | chr2.1:57706158-57706177 | MS.gene058179:intron | 25.0% |
| ! | TATGTCTTTCTATGGTTTAA+TGG | - | chr2.1:57706306-57706325 | MS.gene058179:intron | 25.0% |
| !! | AAAATAATTTTACACCACAG+TGG | + | chr2.1:57706336-57706355 | None:intergenic | 25.0% |
| !! | ATACTTATATTGGTAGTTTC+AGG | - | chr2.1:57706168-57706187 | MS.gene058179:intron | 25.0% |
| !!! | AACAGTTTTTTTTTTTGGTC+AGG | + | chr2.1:57706424-57706443 | None:intergenic | 25.0% |
| !!! | CAAAGTATTGAAATTGTTGA+AGG | - | chr2.1:57705707-57705726 | MS.gene058179:CDS | 25.0% |
| !!! | TTGTTTCCCATATTGTTTTA+CGG | + | chr2.1:57706201-57706220 | None:intergenic | 25.0% |
| AAACCATAGAAAGACATAGA+TGG | + | chr2.1:57706304-57706323 | None:intergenic | 30.0% | |
| AAATTGTCAACCAAATCGAT+CGG | + | chr2.1:57706060-57706079 | None:intergenic | 30.0% | |
| AACGAAAGTAAGTTTCTTGA+TGG | + | chr2.1:57705750-57705769 | None:intergenic | 30.0% | |
| AAGAAACTTACTTTCGTTGA+AGG | - | chr2.1:57705752-57705771 | MS.gene058179:CDS | 30.0% | |
| ACTGTGAAATACTTCACAAA+AGG | - | chr2.1:57706951-57706970 | MS.gene058179:CDS | 30.0% | |
| ATACTAGAGCATGTTTAAGA+TGG | - | chr2.1:57705934-57705953 | MS.gene058179:intron | 30.0% | |
| CAATATAAGTAATCTCATCG+TGG | + | chr2.1:57706132-57706151 | None:intergenic | 30.0% | |
| CACGATGAGATTACTTATAT+TGG | - | chr2.1:57706130-57706149 | MS.gene058179:intron | 30.0% | |
| TTACCATCTATGTCTTTCTA+TGG | - | chr2.1:57706298-57706317 | MS.gene058179:intron | 30.0% | |
| TTTCACAATCACAAACATCA+TGG | + | chr2.1:57706746-57706765 | None:intergenic | 30.0% | |
| ! | AAAGTATGATTTGCACAAAG+TGG | - | chr2.1:57706794-57706813 | MS.gene058179:intron | 30.0% |
| ! | TACTAGAGCATGTTTAAGAT+GGG | - | chr2.1:57705935-57705954 | MS.gene058179:intron | 30.0% |
| !! | ATCAATAACCTTTGGGATAA+GGG | + | chr2.1:57705681-57705700 | None:intergenic | 30.0% |
| !!! | CCGTCTTTATTTTAAGGATA+TGG | + | chr2.1:57705887-57705906 | None:intergenic | 30.0% |
| AACTACTATACCGATCGATT+TGG | - | chr2.1:57706047-57706066 | MS.gene058179:intron | 35.0% | |
| GAAACGTATCAATTATGTGC+AGG | - | chr2.1:57706762-57706781 | MS.gene058179:intron | 35.0% | |
| GAATTGTCATAGTTGTCACA+CGG | + | chr2.1:57706360-57706379 | None:intergenic | 35.0% | |
| TGCAGACAATTTAGACTCAA+AGG | + | chr2.1:57706901-57706920 | None:intergenic | 35.0% | |
| TGCATGCAGTGAAAATTGTA+TGG | + | chr2.1:57705862-57705881 | None:intergenic | 35.0% | |
| TTGAGTCTAAATTGTCTGCA+GGG | - | chr2.1:57706901-57706920 | MS.gene058179:CDS | 35.0% | |
| TTTCACAGTAAGCTTTGCAA+TGG | + | chr2.1:57706940-57706959 | None:intergenic | 35.0% | |
| TTTGAGTCTAAATTGTCTGC+AGG | - | chr2.1:57706900-57706919 | MS.gene058179:CDS | 35.0% | |
| !! | CATCAATAACCTTTGGGATA+AGG | + | chr2.1:57705682-57705701 | None:intergenic | 35.0% |
| ACCAAGTGAAGAGGAAATCA+AGG | - | chr2.1:57706983-57707002 | MS.gene058179:CDS | 40.0% | |
| ACGTATCAATTATGTGCAGG+TGG | - | chr2.1:57706765-57706784 | MS.gene058179:intron | 40.0% | |
| CCAAGTGAAGAGGAAATCAA+GGG | - | chr2.1:57706984-57707003 | MS.gene058179:CDS | 40.0% | |
| GAAATTGTTGAAGGAAACGG+TGG | - | chr2.1:57705716-57705735 | MS.gene058179:CDS | 40.0% | |
| GCTTACAACTACAGCATAGT+TGG | - | chr2.1:57706840-57706859 | MS.gene058179:intron | 40.0% | |
| GGAGACCACTTAGTTCAATT+AGG | + | chr2.1:57706725-57706744 | None:intergenic | 40.0% | |
| GGATGGCATCAATAACCTTT+GGG | + | chr2.1:57705688-57705707 | None:intergenic | 40.0% | |
| TACAACTACAGCATAGTTGG+TGG | - | chr2.1:57706843-57706862 | MS.gene058179:intron | 40.0% | |
| TGGATGGCATCAATAACCTT+TGG | + | chr2.1:57705689-57705708 | None:intergenic | 40.0% | |
| TTCTGATACCCTTATCCCAA+AGG | - | chr2.1:57705670-57705689 | MS.gene058179:CDS | 40.0% | |
| ! | AGAGCTTTGTAAAGTCTAGC+AGG | + | chr2.1:57705641-57705660 | None:intergenic | 40.0% |
| AAATCAAGGGTGGCAAAGCT+AGG | - | chr2.1:57706997-57707016 | MS.gene058179:CDS | 45.0% | |
| AATCAAGGGTGGCAAAGCTA+GGG | - | chr2.1:57706998-57707017 | MS.gene058179:CDS | 45.0% | |
| AGCAGGAGCTACAATAGATG+TGG | + | chr2.1:57705624-57705643 | None:intergenic | 45.0% | |
| AGTGAAGAGGAAATCAAGGG+TGG | - | chr2.1:57706987-57707006 | MS.gene058179:CDS | 45.0% | |
| CCCTTGATTTCCTCTTCACT+TGG | + | chr2.1:57706987-57707006 | None:intergenic | 45.0% | |
| GGTTTAATGGAGATCCACTG+TGG | - | chr2.1:57706319-57706338 | MS.gene058179:intron | 45.0% | |
| TTTGCAATGGATCCTCCATC+TGG | + | chr2.1:57706927-57706946 | None:intergenic | 45.0% | |
| ! | CATGTTTAAGATGGGTCGAC+TGG | - | chr2.1:57705943-57705962 | MS.gene058179:intron | 45.0% |
| ! | GGCTCTTGAAGGTTACGTTT+TGG | - | chr2.1:57707037-57707056 | MS.gene058179:CDS | 45.0% |
| !! | AACTACAGCATAGTTGGTGG+TGG | - | chr2.1:57706846-57706865 | MS.gene058179:intron | 45.0% |
| !! | GGTCTTTTCAAGGCTCTTGA+AGG | - | chr2.1:57707026-57707045 | MS.gene058179:CDS | 45.0% |
| !! | TAGGGGTGATGGTCTTTTCA+AGG | - | chr2.1:57707016-57707035 | MS.gene058179:CDS | 45.0% |
| !! | AATATATAATAAAATAAAAG+AGG | + | chr2.1:57706531-57706550 | None:intergenic | 5.0% |
| !! | ATATATAATAAAATAAAAGA+GGG | + | chr2.1:57706530-57706549 | None:intergenic | 5.0% |
| !!! | TCTTTTATTTTATTATATAT+TGG | - | chr2.1:57706530-57706549 | MS.gene058179:intron | 5.0% |
| AAATTGTCTGCAGGGCCAGA+TGG | - | chr2.1:57706909-57706928 | MS.gene058179:CDS | 50.0% | |
| ATCAAGGGTGGCAAAGCTAG+GGG | - | chr2.1:57706999-57707018 | MS.gene058179:CDS | 50.0% | |
| GAGATCTTCTCCACAGTGTC+TGG | + | chr2.1:57706879-57706898 | None:intergenic | 50.0% | |
| TGATGCTGCACCAAGTGAAG+AGG | - | chr2.1:57706974-57706993 | MS.gene058179:CDS | 50.0% | |
| !! | TACAGCATAGTTGGTGGTGG+TGG | - | chr2.1:57706849-57706868 | MS.gene058179:intron | 50.0% |
| GTTGAAGGAAACGGTGGTGC+TGG | - | chr2.1:57705722-57705741 | MS.gene058179:CDS | 55.0% | |
| !! | TGGTGGACTTCCAGACACTG+TGG | - | chr2.1:57706866-57706885 | MS.gene058179:intron | 55.0% |
| GGTGGCAAAGCTAGGGGTGA+TGG | - | chr2.1:57707005-57707024 | MS.gene058179:CDS | 60.0% | |
| TTGTCTGCAGGGCCAGATGG+AGG | - | chr2.1:57706912-57706931 | MS.gene058179:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 57705593 | 57707076 | 57705593 | ID=MS.gene058179 |
| chr2.1 | mRNA | 57705593 | 57707076 | 57705593 | ID=MS.gene058179.t1;Parent=MS.gene058179 |
| chr2.1 | exon | 57706896 | 57707076 | 57706896 | ID=MS.gene058179.t1.exon1;Parent=MS.gene058179.t1 |
| chr2.1 | CDS | 57706896 | 57707076 | 57706896 | ID=cds.MS.gene058179.t1;Parent=MS.gene058179.t1 |
| chr2.1 | exon | 57705593 | 57705885 | 57705593 | ID=MS.gene058179.t1.exon2;Parent=MS.gene058179.t1 |
| chr2.1 | CDS | 57705593 | 57705885 | 57705593 | ID=cds.MS.gene058179.t1;Parent=MS.gene058179.t1 |
| Gene Sequence |
| Protein sequence |
