Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48475.t1 | XP_003602997.3 | 95.9 | 146 | 6 | 0 | 1 | 146 | 5 | 150 | 2.30E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48475.t1 | A0A396IX21 | 95.9 | 146 | 6 | 0 | 1 | 146 | 5 | 150 | 1.6e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049320 | MS.gene48475 | -0.818487 | 1.91E-52 | -1.69E-46 |
| MS.gene049483 | MS.gene48475 | 0.896108 | 5.06E-76 | -1.69E-46 |
| MS.gene050163 | MS.gene48475 | -0.813093 | 3.05E-51 | -1.69E-46 |
| MS.gene050165 | MS.gene48475 | -0.827208 | 1.78E-54 | -1.69E-46 |
| MS.gene050173 | MS.gene48475 | 0.804522 | 2.07E-49 | -1.69E-46 |
| MS.gene050333 | MS.gene48475 | -0.802336 | 5.89E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene48475 | 0.80578 | 1.13E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene48475 | -0.81626 | 6.06E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene48475 | -0.819152 | 1.35E-52 | -1.69E-46 |
| MS.gene050986 | MS.gene48475 | -0.806359 | 8.55E-50 | -1.69E-46 |
| MS.gene051127 | MS.gene48475 | 0.802535 | 5.36E-49 | -1.69E-46 |
| MS.gene053561 | MS.gene48475 | 0.84055 | 8.13E-58 | -1.69E-46 |
| MS.gene054616 | MS.gene48475 | 0.94148 | 3.86E-101 | -1.69E-46 |
| MS.gene055154 | MS.gene48475 | 0.816411 | 5.61E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene48475 | -0.806677 | 7.32E-50 | -1.69E-46 |
| MS.gene056550 | MS.gene48475 | 0.810782 | 9.71E-51 | -1.69E-46 |
| MS.gene058773 | MS.gene48475 | -0.823358 | 1.45E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene48475 | -0.841124 | 5.75E-58 | -1.69E-46 |
| MS.gene059677 | MS.gene48475 | -0.810155 | 1.33E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene48475 | -0.820782 | 5.72E-53 | -1.69E-46 |
| MS.gene060703 | MS.gene48475 | -0.81571 | 8.04E-52 | -1.69E-46 |
| MS.gene06077 | MS.gene48475 | -0.808813 | 2.58E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48475.t1 | MTR_3g101260 | 96.154 | 130 | 5 | 0 | 17 | 146 | 1 | 130 | 2.82e-91 | 261 |
| MS.gene48475.t1 | MTR_4g086740 | 45.763 | 118 | 64 | 0 | 29 | 146 | 60 | 177 | 1.29e-34 | 119 |
| MS.gene48475.t1 | MTR_4g086730 | 45.763 | 118 | 64 | 0 | 29 | 146 | 61 | 178 | 1.39e-34 | 119 |
| MS.gene48475.t1 | MTR_4g086750 | 45.763 | 118 | 64 | 0 | 29 | 146 | 62 | 179 | 1.51e-34 | 119 |
| MS.gene48475.t1 | MTR_4g086710 | 44.915 | 118 | 65 | 0 | 29 | 146 | 62 | 179 | 2.52e-34 | 118 |
| MS.gene48475.t1 | MTR_4g086690 | 41.379 | 87 | 51 | 0 | 60 | 146 | 21 | 107 | 2.90e-19 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48475.t1 | AT2G15780 | 42.478 | 113 | 63 | 1 | 29 | 141 | 142 | 252 | 4.77e-25 | 96.7 |
| MS.gene48475.t1 | AT2G15770 | 38.793 | 116 | 69 | 1 | 26 | 141 | 141 | 254 | 1.17e-23 | 94.0 |
| MS.gene48475.t1 | AT4G33930 | 35.897 | 117 | 58 | 6 | 39 | 144 | 229 | 339 | 4.59e-12 | 62.4 |
| MS.gene48475.t1 | AT4G34300 | 35.652 | 115 | 56 | 6 | 39 | 141 | 198 | 306 | 1.58e-11 | 60.8 |
Find 47 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCCAATGTGTCATTTATT+TGG | 0.160335 | 3.1:-80401083 | None:intergenic |
| CTTCCTTGTGATTGCTTCAA+TGG | 0.264782 | 3.1:+80400948 | MS.gene48475:CDS |
| TCCAACCAAGATGCTTCTTT+TGG | 0.296074 | 3.1:-80400998 | None:intergenic |
| GTGATTGCTTCAATGGTTGC+AGG | 0.311734 | 3.1:+80400955 | MS.gene48475:CDS |
| GGGAATTGGACTAAATTAAA+AGG | 0.331947 | 3.1:-80401582 | None:intergenic |
| TCATCAGAGGCAAAGTAATA+AGG | 0.345121 | 3.1:-80401497 | None:intergenic |
| TACACGACGTGTGAATTTAG+AGG | 0.385924 | 3.1:+80401404 | MS.gene48475:CDS |
| GCTTCAATGGTTGCAGGGTC+AGG | 0.401233 | 3.1:+80400961 | MS.gene48475:CDS |
| GACTCTGAAGGTTGGCGTGC+AGG | 0.411668 | 3.1:+80401021 | MS.gene48475:CDS |
| AATTAAAAGGAACGTGTAGA+TGG | 0.428002 | 3.1:-80401569 | None:intergenic |
| TATTACTTTGCCTCTGATGA+AGG | 0.434564 | 3.1:+80401500 | MS.gene48475:CDS |
| AATGCCTATGACTGTATTGC+TGG | 0.454610 | 3.1:+80401524 | MS.gene48475:CDS |
| GAGTCCAGCAATACAGTCAT+AGG | 0.464321 | 3.1:-80401528 | None:intergenic |
| GTCGTGTAACTCCACATATT+CGG | 0.471065 | 3.1:-80401389 | None:intergenic |
| CAATGTGTCATTTATTTGGA+AGG | 0.485349 | 3.1:-80401079 | None:intergenic |
| TTTAGAGGAGCAAAGTTGCT+TGG | 0.490765 | 3.1:+80401419 | MS.gene48475:CDS |
| TTTACTTGCTACCGAATATG+TGG | 0.498287 | 3.1:+80401378 | MS.gene48475:CDS |
| TTCCAAATAAATGACACATT+GGG | 0.508285 | 3.1:+80401081 | MS.gene48475:CDS |
| ATGTGTCATTTATTTGGAAG+GGG | 0.525400 | 3.1:-80401077 | None:intergenic |
| AATGTGTCATTTATTTGGAA+GGG | 0.530429 | 3.1:-80401078 | None:intergenic |
| CTTCCAAATAAATGACACAT+TGG | 0.530831 | 3.1:+80401080 | MS.gene48475:CDS |
| CAGAACCAAAAGAAGCATCT+TGG | 0.539687 | 3.1:+80400993 | MS.gene48475:CDS |
| ACCAAAAGAAGCATCTTGGT+TGG | 0.551375 | 3.1:+80400997 | MS.gene48475:CDS |
| GGTTGCAGGGTCAGGAAGCA+AGG | 0.552711 | 3.1:+80400969 | MS.gene48475:CDS |
| CAGGCACTAACTACACCCAA+TGG | 0.558303 | 3.1:+80401040 | MS.gene48475:CDS |
| GCTGATCAAGGTGGTGGTGA+GGG | 0.565763 | 3.1:+80401446 | MS.gene48475:CDS |
| TGATTGCTTCAATGGTTGCA+GGG | 0.566600 | 3.1:+80400956 | MS.gene48475:CDS |
| TTCGGTAGCAAGTAAACGCT+GGG | 0.568902 | 3.1:-80401371 | None:intergenic |
| TGGTTGGAGACTCTGAAGGT+TGG | 0.582794 | 3.1:+80401013 | MS.gene48475:CDS |
| AGGCACTAACTACACCCAAT+GGG | 0.584087 | 3.1:+80401041 | MS.gene48475:CDS |
| ATTCGGTAGCAAGTAAACGC+TGG | 0.587303 | 3.1:-80401372 | None:intergenic |
| AACCGTGGAATTTCCTGAAG+GGG | 0.598371 | 3.1:-80401345 | None:intergenic |
| AGATGGAACAGCAATGAACT+TGG | 0.599581 | 3.1:-80401552 | None:intergenic |
| TGCTGATCAAGGTGGTGGTG+AGG | 0.607379 | 3.1:+80401445 | MS.gene48475:CDS |
| ATAGGCATTGCCTTCATCAG+AGG | 0.610448 | 3.1:-80401510 | None:intergenic |
| TAAAGATTGAGCTGAGTCAG+TGG | 0.610528 | 3.1:+80401471 | MS.gene48475:CDS |
| ATGTCTAGAATTCTCATACA+AGG | 0.613782 | 3.1:+80400919 | MS.gene48475:CDS |
| GGTAATGCTGATCAAGGTGG+TGG | 0.619304 | 3.1:+80401440 | MS.gene48475:CDS |
| ACCGTGGAATTTCCTGAAGG+GGG | 0.620984 | 3.1:-80401344 | None:intergenic |
| TTGCTTGGTAATGCTGATCA+AGG | 0.621103 | 3.1:+80401434 | MS.gene48475:CDS |
| ATCTTGGTTGGAGACTCTGA+AGG | 0.629130 | 3.1:+80401009 | MS.gene48475:CDS |
| CAACCATTGAAGCAATCACA+AGG | 0.631574 | 3.1:-80400951 | None:intergenic |
| CAACCGTGGAATTTCCTGAA+GGG | 0.654328 | 3.1:-80401346 | None:intergenic |
| ACAACCGTGGAATTTCCTGA+AGG | 0.668113 | 3.1:-80401347 | None:intergenic |
| CTTGGTAATGCTGATCAAGG+TGG | 0.670664 | 3.1:+80401437 | MS.gene48475:CDS |
| GTAAACGCTGGGAACAACCG+TGG | 0.691075 | 3.1:-80401360 | None:intergenic |
| CCGTGGAATTTCCTGAAGGG+GGG | 0.694717 | 3.1:-80401343 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTCAAATTATTATCAA+AGG | + | chr3.1:80401211-80401230 | MS.gene48475:intron | 10.0% |
| !! | TTAAATATAATTAAATGCGT+TGG | - | chr3.1:80401182-80401201 | None:intergenic | 15.0% |
| !!! | TTTGATTGATGGATTAATTT+TGG | + | chr3.1:80401286-80401305 | MS.gene48475:intron | 20.0% |
| ! | CAATATATATGCACAAACAA+TGG | - | chr3.1:80401249-80401268 | None:intergenic | 25.0% |
| ! | TTCCAAATAAATGACACATT+GGG | + | chr3.1:80401081-80401100 | MS.gene48475:CDS | 25.0% |
| !!! | AATGTGTCATTTATTTGGAA+GGG | - | chr3.1:80401081-80401100 | None:intergenic | 25.0% |
| CTTCCAAATAAATGACACAT+TGG | + | chr3.1:80401080-80401099 | MS.gene48475:CDS | 30.0% | |
| CTTCTTCTTCATTTGATTGA+TGG | + | chr3.1:80401275-80401294 | MS.gene48475:intron | 30.0% | |
| ! | ATGTCTAGAATTCTCATACA+AGG | + | chr3.1:80400919-80400938 | MS.gene48475:CDS | 30.0% |
| !! | ATGTGTCATTTATTTGGAAG+GGG | - | chr3.1:80401080-80401099 | None:intergenic | 30.0% |
| !! | CAATGTGTCATTTATTTGGA+AGG | - | chr3.1:80401082-80401101 | None:intergenic | 30.0% |
| !! | TACCCAATGTGTCATTTATT+TGG | - | chr3.1:80401086-80401105 | None:intergenic | 30.0% |
| TATTACTTTGCCTCTGATGA+AGG | + | chr3.1:80401500-80401519 | MS.gene48475:CDS | 35.0% | |
| TCATCAGAGGCAAAGTAATA+AGG | - | chr3.1:80401500-80401519 | None:intergenic | 35.0% | |
| ! | TTTACTTGCTACCGAATATG+TGG | + | chr3.1:80401378-80401397 | MS.gene48475:CDS | 35.0% |
| !!! | GCTATTTTTTATGGCCCATT+GGG | - | chr3.1:80401058-80401077 | None:intergenic | 35.0% |
| !!! | TTGGAAGGGGCTATTTTTTA+TGG | - | chr3.1:80401067-80401086 | None:intergenic | 35.0% |
| AATGCCTATGACTGTATTGC+TGG | + | chr3.1:80401524-80401543 | MS.gene48475:CDS | 40.0% | |
| AGATGGAACAGCAATGAACT+TGG | - | chr3.1:80401555-80401574 | None:intergenic | 40.0% | |
| CAACCATTGAAGCAATCACA+AGG | - | chr3.1:80400954-80400973 | None:intergenic | 40.0% | |
| CTTCCTTGTGATTGCTTCAA+TGG | + | chr3.1:80400948-80400967 | MS.gene48475:CDS | 40.0% | |
| GTCGTGTAACTCCACATATT+CGG | - | chr3.1:80401392-80401411 | None:intergenic | 40.0% | |
| TAAAGATTGAGCTGAGTCAG+TGG | + | chr3.1:80401471-80401490 | MS.gene48475:CDS | 40.0% | |
| TACACGACGTGTGAATTTAG+AGG | + | chr3.1:80401404-80401423 | MS.gene48475:CDS | 40.0% | |
| TGATTGCTTCAATGGTTGCA+GGG | + | chr3.1:80400956-80400975 | MS.gene48475:CDS | 40.0% | |
| TTTAGAGGAGCAAAGTTGCT+TGG | + | chr3.1:80401419-80401438 | MS.gene48475:CDS | 40.0% | |
| ! | TCCAACCAAGATGCTTCTTT+TGG | - | chr3.1:80401001-80401020 | None:intergenic | 40.0% |
| !! | ACCAAAAGAAGCATCTTGGT+TGG | + | chr3.1:80400997-80401016 | MS.gene48475:CDS | 40.0% |
| !! | CAGAACCAAAAGAAGCATCT+TGG | + | chr3.1:80400993-80401012 | MS.gene48475:CDS | 40.0% |
| !! | TTGCTTGGTAATGCTGATCA+AGG | + | chr3.1:80401434-80401453 | MS.gene48475:CDS | 40.0% |
| !!! | CAAGATGCTTCTTTTGGTTC+TGG | - | chr3.1:80400995-80401014 | None:intergenic | 40.0% |
| !!! | GGCTATTTTTTATGGCCCAT+TGG | - | chr3.1:80401059-80401078 | None:intergenic | 40.0% |
| AACCGTGGAATTTCCTGAAG+GGG | - | chr3.1:80401348-80401367 | None:intergenic | 45.0% | |
| ACAACCGTGGAATTTCCTGA+AGG | - | chr3.1:80401350-80401369 | None:intergenic | 45.0% | |
| AGGCACTAACTACACCCAAT+GGG | + | chr3.1:80401041-80401060 | MS.gene48475:CDS | 45.0% | |
| ATCTTGGTTGGAGACTCTGA+AGG | + | chr3.1:80401009-80401028 | MS.gene48475:CDS | 45.0% | |
| ATTCGGTAGCAAGTAAACGC+TGG | - | chr3.1:80401375-80401394 | None:intergenic | 45.0% | |
| CAACCGTGGAATTTCCTGAA+GGG | - | chr3.1:80401349-80401368 | None:intergenic | 45.0% | |
| GAGTCCAGCAATACAGTCAT+AGG | - | chr3.1:80401531-80401550 | None:intergenic | 45.0% | |
| GTGATTGCTTCAATGGTTGC+AGG | + | chr3.1:80400955-80400974 | MS.gene48475:CDS | 45.0% | |
| TTCGGTAGCAAGTAAACGCT+GGG | - | chr3.1:80401374-80401393 | None:intergenic | 45.0% | |
| ! | ATAGGCATTGCCTTCATCAG+AGG | - | chr3.1:80401513-80401532 | None:intergenic | 45.0% |
| !! | CTTGGTAATGCTGATCAAGG+TGG | + | chr3.1:80401437-80401456 | MS.gene48475:CDS | 45.0% |
| ACCGTGGAATTTCCTGAAGG+GGG | - | chr3.1:80401347-80401366 | None:intergenic | 50.0% | |
| CAGGCACTAACTACACCCAA+TGG | + | chr3.1:80401040-80401059 | MS.gene48475:CDS | 50.0% | |
| GGTAATGCTGATCAAGGTGG+TGG | + | chr3.1:80401440-80401459 | MS.gene48475:CDS | 50.0% | |
| ! | TGGTTGGAGACTCTGAAGGT+TGG | + | chr3.1:80401013-80401032 | MS.gene48475:CDS | 50.0% |
| CCCCCCTTCAGGAAATTCCA+CGG | + | chr3.1:80401343-80401362 | MS.gene48475:CDS | 55.0% | |
| CCGTGGAATTTCCTGAAGGG+GGG | - | chr3.1:80401346-80401365 | None:intergenic | 55.0% | |
| GCTTCAATGGTTGCAGGGTC+AGG | + | chr3.1:80400961-80400980 | MS.gene48475:CDS | 55.0% | |
| GTAAACGCTGGGAACAACCG+TGG | - | chr3.1:80401363-80401382 | None:intergenic | 55.0% | |
| GTGTTCAAGTACCCCCCTTC+AGG | + | chr3.1:80401332-80401351 | MS.gene48475:intron | 55.0% | |
| ! | GCTGATCAAGGTGGTGGTGA+GGG | + | chr3.1:80401446-80401465 | MS.gene48475:CDS | 55.0% |
| ! | TGCTGATCAAGGTGGTGGTG+AGG | + | chr3.1:80401445-80401464 | MS.gene48475:CDS | 55.0% |
| GGTTGCAGGGTCAGGAAGCA+AGG | + | chr3.1:80400969-80400988 | MS.gene48475:CDS | 60.0% | |
| ! | GACTCTGAAGGTTGGCGTGC+AGG | + | chr3.1:80401021-80401040 | MS.gene48475:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 80400919 | 80401589 | 80400919 | ID=MS.gene48475 |
| chr3.1 | mRNA | 80400919 | 80401589 | 80400919 | ID=MS.gene48475.t1;Parent=MS.gene48475 |
| chr3.1 | exon | 80400919 | 80401102 | 80400919 | ID=MS.gene48475.t1.exon1;Parent=MS.gene48475.t1 |
| chr3.1 | CDS | 80400919 | 80401102 | 80400919 | ID=cds.MS.gene48475.t1;Parent=MS.gene48475.t1 |
| chr3.1 | exon | 80401333 | 80401589 | 80401333 | ID=MS.gene48475.t1.exon2;Parent=MS.gene48475.t1 |
| chr3.1 | CDS | 80401333 | 80401589 | 80401333 | ID=cds.MS.gene48475.t1;Parent=MS.gene48475.t1 |
| Gene Sequence |
| Protein sequence |
