Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48858.t1 | XP_028762943.1 | 66 | 94 | 26 | 1 | 12 | 99 | 255 | 348 | 1.10E-27 | 132.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48858.t1 | A0A151TBS9 | 64.8 | 88 | 30 | 1 | 10 | 97 | 136 | 222 | 1.2e-25 | 125.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene48858 | 0.835833 | 1.33E-56 | -1.69E-46 |
| MS.gene049701 | MS.gene48858 | 0.812495 | 4.12E-51 | -1.69E-46 |
| MS.gene049853 | MS.gene48858 | 0.810248 | 1.27E-50 | -1.69E-46 |
| MS.gene05329 | MS.gene48858 | 0.811631 | 6.36E-51 | -1.69E-46 |
| MS.gene05422 | MS.gene48858 | 0.802684 | 4.99E-49 | -1.69E-46 |
| MS.gene055056 | MS.gene48858 | 0.8128 | 3.53E-51 | -1.69E-46 |
| MS.gene055058 | MS.gene48858 | 0.817927 | 2.56E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene48858 | 0.826174 | 3.13E-54 | -1.69E-46 |
| MS.gene05762 | MS.gene48858 | 0.800624 | 1.32E-48 | -1.69E-46 |
| MS.gene058515 | MS.gene48858 | 0.828014 | 1.14E-54 | -1.69E-46 |
| MS.gene059276 | MS.gene48858 | 0.952359 | 2.87E-110 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48858.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 19 | 99 | 5 | 80 | 1.91e-24 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCTTTGCTGATCCTTA+TGG | 0.222807 | 4.3:-71554053 | MS.gene48858:CDS |
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 4.3:-71554151 | MS.gene48858:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 4.3:-71554159 | MS.gene48858:CDS |
| AGAAATCAGCAGCATCTAAA+TGG | 0.308817 | 4.3:+71554069 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 4.3:+71554182 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 4.3:-71554099 | MS.gene48858:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.394855 | 4.3:-71554107 | MS.gene48858:CDS |
| GGAATGATTGATCATCTCAT+TGG | 0.401226 | 4.3:-71554032 | MS.gene48858:CDS |
| GTTGTGGAGGCAAAGGTCAT+TGG | 0.462627 | 4.3:-71554223 | MS.gene48858:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 4.3:-71554150 | MS.gene48858:CDS |
| TGCTATCGTTGTGGAGGCAA+AGG | 0.489090 | 4.3:-71554230 | MS.gene48858:CDS |
| AGATGATCAATCATTCCATA+AGG | 0.536978 | 4.3:+71554038 | None:intergenic |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 4.3:-71554239 | MS.gene48858:CDS |
| AGTATTTGCTATCGTTGTGG+AGG | 0.613176 | 4.3:-71554236 | MS.gene48858:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGAAAAAAATGATAT+TGG | - | chr4.3:71554016-71554035 | MS.gene48858:CDS | 15.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr4.3:71554147-71554166 | MS.gene48858:CDS | 20.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr4.3:71554146-71554165 | MS.gene48858:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | - | chr4.3:71554190-71554209 | MS.gene48858:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | - | chr4.3:71554198-71554217 | MS.gene48858:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | + | chr4.3:71554118-71554137 | None:intergenic | 25.0% |
| AGATGATCAATCATTCCATA+AGG | + | chr4.3:71554262-71554281 | None:intergenic | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr4.3:71554138-71554157 | MS.gene48858:CDS | 30.0% | |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr4.3:71554141-71554160 | None:intergenic | 30.0% |
| AGAAATCAGCAGCATCTAAA+TGG | + | chr4.3:71554231-71554250 | None:intergenic | 35.0% | |
| GATTTCTTTGCTGATCCTTA+TGG | - | chr4.3:71554244-71554263 | MS.gene48858:CDS | 35.0% | |
| GGAATGATTGATCATCTCAT+TGG | - | chr4.3:71554265-71554284 | MS.gene48858:CDS | 35.0% | |
| !! | AGTATTTGCTATCGTTGTGG+AGG | - | chr4.3:71554061-71554080 | MS.gene48858:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr4.3:71554058-71554077 | MS.gene48858:CDS | 40.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | + | chr4.3:71554107-71554126 | None:intergenic | 45.0% |
| GTTGTGGAGGCAAAGGTCAT+TGG | - | chr4.3:71554074-71554093 | MS.gene48858:CDS | 50.0% | |
| TGCTATCGTTGTGGAGGCAA+AGG | - | chr4.3:71554067-71554086 | MS.gene48858:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 71554010 | 71554309 | 71554010 | ID=MS.gene48858 |
| chr4.3 | mRNA | 71554010 | 71554309 | 71554010 | ID=MS.gene48858.t1;Parent=MS.gene48858 |
| chr4.3 | exon | 71554010 | 71554309 | 71554010 | ID=MS.gene48858.t1.exon1;Parent=MS.gene48858.t1 |
| chr4.3 | CDS | 71554010 | 71554309 | 71554010 | ID=cds.MS.gene48858.t1;Parent=MS.gene48858.t1 |
| Gene Sequence |
| Protein sequence |
