AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05762


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05762.t1 MTR_3g465410 93.617 141 9 0 14 154 3 143 1.74e-95 272
MS.gene05762.t1 MTR_4g063790 90.780 141 13 0 14 154 31 171 1.27e-90 261
MS.gene05762.t1 MTR_3g467080 77.099 131 30 0 14 144 31 161 3.29e-68 204
MS.gene05762.t1 MTR_3g466980 49.296 142 69 2 14 153 31 171 2.39e-41 137
MS.gene05762.t1 MTR_3g031240 46.970 132 70 0 15 146 32 163 4.34e-37 125
MS.gene05762.t1 MTR_3g065100 47.244 127 66 1 15 141 32 157 2.80e-36 125
MS.gene05762.t1 MTR_2g016210 43.165 139 79 0 14 152 31 169 3.97e-35 122
MS.gene05762.t1 MTR_3g031100 41.727 139 81 0 14 152 31 169 1.47e-32 115
MS.gene05762.t1 MTR_4g032620 39.259 135 82 0 14 148 31 165 8.47e-31 111
MS.gene05762.t1 MTR_5g075380 40.000 130 71 2 14 140 31 156 1.91e-30 108
MS.gene05762.t1 MTR_4g032290 39.552 134 81 0 15 148 32 165 2.06e-30 110
MS.gene05762.t1 MTR_2g035580 46.774 124 65 1 14 137 31 153 5.60e-29 104
MS.gene05762.t1 MTR_4g031910 38.806 134 82 0 15 148 32 165 6.16e-29 106
MS.gene05762.t1 MTR_4g032260 36.508 126 80 0 17 142 34 159 9.84e-28 101
MS.gene05762.t1 MTR_8g036130 38.686 137 82 1 14 148 31 167 3.63e-26 99.8
MS.gene05762.t1 MTR_2g035610 46.512 86 46 0 15 100 32 117 3.48e-25 93.6
MS.gene05762.t1 MTR_1g077300 35.385 130 80 2 15 144 32 157 2.15e-22 87.4
MS.gene05762.t1 MTR_1g077320 36.719 128 79 2 15 142 32 157 2.21e-22 87.4
MS.gene05762.t1 MTR_4g028720 35.938 128 80 2 15 142 32 157 2.77e-22 87.4
MS.gene05762.t1 MTR_5g047580 37.903 124 75 2 14 137 31 152 4.17e-22 86.7
MS.gene05762.t1 MTR_7g011950 35.156 128 81 2 15 142 32 157 1.16e-21 85.5
MS.gene05762.t1 MTR_1g084950 34.127 126 81 2 15 140 32 155 2.16e-21 84.7
MS.gene05762.t1 MTR_3g466830 34.532 139 90 1 2 140 18 155 2.49e-21 84.7
MS.gene05762.t1 MTR_1g077390 36.434 129 80 2 14 142 31 157 5.28e-21 84.0
MS.gene05762.t1 MTR_3g466930 34.921 126 81 1 15 140 2 126 6.36e-20 80.5
MS.gene05762.t1 MTR_1g090783 34.677 124 79 2 14 137 30 151 6.40e-19 78.6
MS.gene05762.t1 MTR_3g466900 33.333 126 83 1 15 140 2 126 3.65e-18 75.9
MS.gene05762.t1 MTR_3g466890 33.588 131 86 1 10 140 26 155 1.50e-17 75.1
MS.gene05762.t1 MTR_7g055800 37.931 87 39 1 14 100 18 89 1.55e-15 67.8
MS.gene05762.t1 MTR_1g090710 31.452 124 84 1 14 137 31 153 5.76e-15 68.2
MS.gene05762.t1 MTR_5g047560 35.398 113 71 2 25 137 26 136 3.17e-14 65.9
MS.gene05762.t1 MTR_1g090697 32.540 126 79 4 14 137 31 152 5.04e-14 65.9
MS.gene05762.t1 MTR_7g055940 38.158 76 32 1 14 89 18 78 9.48e-14 63.2
MS.gene05762.t1 MTR_4g019670 33.333 108 62 2 14 118 34 134 3.10e-12 63.5
MS.gene05762.t1 MTR_7g106510 32.143 112 61 3 14 122 34 133 6.15e-12 62.8
MS.gene05762.t1 MTR_4g084780 53.704 54 25 0 57 110 1 54 7.55e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05762.t1 AT5G48670 45.255 137 69 4 15 149 32 164 5.36e-31 114
MS.gene05762.t1 AT5G27810 51.724 87 42 0 14 100 3 89 8.60e-28 100
MS.gene05762.t1 AT1G65300 39.200 125 75 1 14 138 30 153 3.24e-22 89.7
MS.gene05762.t1 AT1G65330 39.200 125 75 1 14 138 30 153 2.32e-21 87.8
MS.gene05762.t1 AT3G05860 40.164 122 68 2 14 133 31 149 2.56e-20 83.6
MS.gene05762.t1 AT3G05860 36.620 142 85 2 14 153 31 169 3.44e-20 84.0
MS.gene05762.t1 AT3G05860 36.620 142 85 2 14 153 31 169 5.38e-20 83.6
MS.gene05762.t1 AT5G26630 35.537 121 77 1 17 137 34 153 6.25e-20 82.8
MS.gene05762.t1 AT5G26650 34.351 131 77 2 14 140 29 154 2.59e-19 83.2
MS.gene05762.t1 AT1G31640 36.029 136 73 4 12 141 28 155 5.20e-19 82.8
MS.gene05762.t1 AT5G27960 43.373 83 47 0 14 96 29 111 2.14e-17 77.4
MS.gene05762.t1 AT1G31630 33.333 129 82 3 14 141 30 155 7.56e-17 76.3
MS.gene05762.t1 AT1G22590 37.349 83 52 0 14 96 31 113 1.29e-14 67.4
MS.gene05762.t1 AT2G28700 32.258 124 75 2 14 133 31 149 1.31e-11 61.6
MS.gene05762.t1 AT5G06500 27.559 127 84 3 14 137 31 152 5.62e-11 58.9

Find 36 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCATCTTTC+TGG 0.077675 3.1:+49188930 None:intergenic
ATTCAAACCATAGAATTTAA+TGG 0.173791 3.1:-49188892 MS.gene05762:CDS
TACATGCTTTGTTGAAATTA+TGG 0.174609 3.1:+49189384 None:intergenic
CGAATTTCCATTAAATTCTA+TGG 0.218755 3.1:+49188885 None:intergenic
GAGGTTTGGCCATCAGATTC+AGG 0.258145 3.1:-49189087 MS.gene05762:CDS
TCTTCTTGAGGCAAAACATT+AGG 0.355934 3.1:-49188990 MS.gene05762:CDS
AATATAATGATCAATGATAT+TGG 0.368342 3.1:+49188912 None:intergenic
ATCAAGAGGAGGTTGGAAAT+GGG 0.388178 3.1:-49188771 MS.gene05762:CDS
GATCAAGAGGAGGTTGGAAA+TGG 0.401299 3.1:-49188772 MS.gene05762:CDS
GAAGTGTGATATCTAGGTTT+AGG 0.426765 3.1:-49189059 MS.gene05762:CDS
GGGTCAGAAGTGTGATATCT+AGG 0.436600 3.1:-49189065 MS.gene05762:CDS
TCTAGGTTTAGGAGTTTGCC+TGG 0.445921 3.1:-49189048 MS.gene05762:CDS
AGAATTTGAAGGAGGTTGAT+CGG 0.478161 3.1:-49188819 MS.gene05762:CDS
ACAACCCTCGAGTAGAGGTT+TGG 0.487846 3.1:-49189101 MS.gene05762:CDS
TCAAGAGGAGGTTGGAAATG+GGG 0.500358 3.1:-49188770 MS.gene05762:CDS
AGGTTTGGCCATCAGATTCA+GGG 0.511068 3.1:-49189086 MS.gene05762:CDS
ACGAGCAGTTGAAGAAGCAA+AGG 0.512962 3.1:-49188960 MS.gene05762:CDS
AAGGGAGGAAACCAGAAAGA+TGG 0.513695 3.1:-49188941 MS.gene05762:CDS
ATGAAAGATCAAGAGGAGGT+TGG 0.519383 3.1:-49188778 MS.gene05762:CDS
GAGGAGGTTGGAAATGGGGT+GGG 0.563227 3.1:-49188766 MS.gene05762:CDS
AGAGGAGGTTGGAAATGGGG+TGG 0.565910 3.1:-49188767 MS.gene05762:CDS
CGAGCAGTTGAAGAAGCAAA+GGG 0.580872 3.1:-49188959 MS.gene05762:CDS
ACATGCTTCGACTCCACATA+GGG 0.581479 3.1:+49189143 None:intergenic
AATGACAATGAAAGATCAAG+AGG 0.583122 3.1:-49188785 MS.gene05762:CDS
TATTGATGAGAATTTGAAGG+AGG 0.592173 3.1:-49188827 MS.gene05762:CDS
CACATGCTTCGACTCCACAT+AGG 0.603214 3.1:+49189142 None:intergenic
AATGAAATTAGCACCCTATG+TGG 0.604835 3.1:-49189156 MS.gene05762:CDS
GCAAAACAACCCTCGAGTAG+AGG 0.609086 3.1:-49189106 MS.gene05762:CDS
CTTCTGACCCCTGAATCTGA+TGG 0.624024 3.1:+49189078 None:intergenic
GATGGCCAAACCTCTACTCG+AGG 0.634480 3.1:+49189096 None:intergenic
GACAATGAAAGATCAAGAGG+AGG 0.653972 3.1:-49188782 MS.gene05762:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.657992 3.1:-49189085 MS.gene05762:CDS
ATGGCCAAACCTCTACTCGA+GGG 0.659257 3.1:+49189097 None:intergenic
GCTTTGTTGAAATTATGGAG+AGG 0.672067 3.1:+49189389 None:intergenic
GCAGTTGAAGAAGCAAAGGG+AGG 0.693727 3.1:-49188956 MS.gene05762:CDS
TGGATCAGGAGGTCTTCTTG+AGG 0.700337 3.1:-49189002 MS.gene05762:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATATAGAATTTTTTTAAT+AGG - chr3.1:49188967-49188986 MS.gene05762:CDS 10.0%
!! AATATAATGATCAATGATAT+TGG + chr3.1:49189253-49189272 None:intergenic 15.0%
!! AATAAACAATATACAATCGA+TGG + chr3.1:49188948-49188967 None:intergenic 20.0%
!! ATTCAAACCATAGAATTTAA+TGG - chr3.1:49189270-49189289 MS.gene05762:intron 20.0%
!!! TTTTATTGATGAGAATTTGA+AGG - chr3.1:49189332-49189351 MS.gene05762:intron 20.0%
! ACATGAACAAAGTAAAAAGA+TGG - chr3.1:49189137-49189156 MS.gene05762:CDS 25.0%
! CGAATTTCCATTAAATTCTA+TGG + chr3.1:49189280-49189299 None:intergenic 25.0%
! TGTTAACATGTGATATTTCT+AGG + chr3.1:49188841-49188860 None:intergenic 25.0%
!! TACATGCTTTGTTGAAATTA+TGG + chr3.1:49188781-49188800 None:intergenic 25.0%
!!! TGTGATATTTCTAGGAAAAA+AGG + chr3.1:49188833-49188852 None:intergenic 25.0%
AATGACAATGAAAGATCAAG+AGG - chr3.1:49189377-49189396 MS.gene05762:intron 30.0%
TATTGATGAGAATTTGAAGG+AGG - chr3.1:49189335-49189354 MS.gene05762:intron 30.0%
TGAACAAAGTAAAAAGATGG+TGG - chr3.1:49189140-49189159 MS.gene05762:CDS 30.0%
! TTTTTACTTTGTTCATGTCC+AGG + chr3.1:49189135-49189154 None:intergenic 30.0%
AATGAAATTAGCACCCTATG+TGG - chr3.1:49189006-49189025 MS.gene05762:CDS 35.0%
ATTGGTCATTTCCATCTTTC+TGG + chr3.1:49189235-49189254 None:intergenic 35.0%
GAAGTGTGATATCTAGGTTT+AGG - chr3.1:49189103-49189122 MS.gene05762:CDS 35.0%
TCTTCTTGAGGCAAAACATT+AGG - chr3.1:49189172-49189191 MS.gene05762:intron 35.0%
! AGAATTTGAAGGAGGTTGAT+CGG - chr3.1:49189343-49189362 MS.gene05762:intron 35.0%
! GCTTTGTTGAAATTATGGAG+AGG + chr3.1:49188776-49188795 None:intergenic 35.0%
!! AAGTAAAAAGATGGTGGATC+AGG - chr3.1:49189146-49189165 MS.gene05762:CDS 35.0%
ATCAAGAGGAGGTTGGAAAT+GGG - chr3.1:49189391-49189410 MS.gene05762:CDS 40.0%
ATGAAAGATCAAGAGGAGGT+TGG - chr3.1:49189384-49189403 MS.gene05762:intron 40.0%
GACAATGAAAGATCAAGAGG+AGG - chr3.1:49189380-49189399 MS.gene05762:intron 40.0%
!! TAAAAAGATGGTGGATCAGG+AGG - chr3.1:49189149-49189168 MS.gene05762:CDS 40.0%
AAGGGAGGAAACCAGAAAGA+TGG - chr3.1:49189221-49189240 MS.gene05762:intron 45.0%
ACATGCTTCGACTCCACATA+GGG + chr3.1:49189022-49189041 None:intergenic 45.0%
ACGAGCAGTTGAAGAAGCAA+AGG - chr3.1:49189202-49189221 MS.gene05762:intron 45.0%
AGGTTTGGCCATCAGATTCA+GGG - chr3.1:49189076-49189095 MS.gene05762:CDS 45.0%
CGAGCAGTTGAAGAAGCAAA+GGG - chr3.1:49189203-49189222 MS.gene05762:intron 45.0%
GATCAAGAGGAGGTTGGAAA+TGG - chr3.1:49189390-49189409 MS.gene05762:CDS 45.0%
TCAAGAGGAGGTTGGAAATG+GGG - chr3.1:49189392-49189411 MS.gene05762:CDS 45.0%
TCTAGGTTTAGGAGTTTGCC+TGG - chr3.1:49189114-49189133 MS.gene05762:CDS 45.0%
! GGGTCAGAAGTGTGATATCT+AGG - chr3.1:49189097-49189116 MS.gene05762:CDS 45.0%
ACAACCCTCGAGTAGAGGTT+TGG - chr3.1:49189061-49189080 MS.gene05762:CDS 50.0%
ATGGCCAAACCTCTACTCGA+GGG + chr3.1:49189068-49189087 None:intergenic 50.0%
CACATGCTTCGACTCCACAT+AGG + chr3.1:49189023-49189042 None:intergenic 50.0%
CTTCTGACCCCTGAATCTGA+TGG + chr3.1:49189087-49189106 None:intergenic 50.0%
GAGGTTTGGCCATCAGATTC+AGG - chr3.1:49189075-49189094 MS.gene05762:CDS 50.0%
GCAAAACAACCCTCGAGTAG+AGG - chr3.1:49189056-49189075 MS.gene05762:CDS 50.0%
GCAGTTGAAGAAGCAAAGGG+AGG - chr3.1:49189206-49189225 MS.gene05762:intron 50.0%
GGTTTGGCCATCAGATTCAG+GGG - chr3.1:49189077-49189096 MS.gene05762:CDS 50.0%
TGGATCAGGAGGTCTTCTTG+AGG - chr3.1:49189160-49189179 MS.gene05762:CDS 50.0%
AGAGGAGGTTGGAAATGGGG+TGG - chr3.1:49189395-49189414 MS.gene05762:CDS 55.0%
GAGGAGGTTGGAAATGGGGT+GGG - chr3.1:49189396-49189415 MS.gene05762:CDS 55.0%
GATGGCCAAACCTCTACTCG+AGG + chr3.1:49189069-49189088 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 49188759 49189425 49188759 ID=MS.gene05762
chr3.1 mRNA 49188759 49189425 49188759 ID=MS.gene05762.t1;Parent=MS.gene05762
chr3.1 exon 49189387 49189425 49189387 ID=MS.gene05762.t1.exon1;Parent=MS.gene05762.t1
chr3.1 CDS 49189387 49189425 49189387 ID=cds.MS.gene05762.t1;Parent=MS.gene05762.t1
chr3.1 exon 49188759 49189184 49188759 ID=MS.gene05762.t1.exon2;Parent=MS.gene05762.t1
chr3.1 CDS 49188759 49189184 49188759 ID=cds.MS.gene05762.t1;Parent=MS.gene05762.t1
Gene Sequence

>MS.gene05762

ATGGTTAGATTTAACCTCTCCATAATTTCAACAAAGCATGTATATATTATCTTTGCATATCATACACATAGCCTTTTTTCCTAGAAATATCACATGTTAACATTTTTTGTTTATATTTATTAAAACTATAATTTATAGTTTTGAAAATTCGTGATTTAGATTTCATTTAGTGAATTTGAAATTTTACCATCGATTGTATATTGTTTATTGATATAGAATTTTTTTAATAGGAATTATGAAGAAGATTAATGAAATTAGCACCCTATGTGGAGTCGAAGCATGTGCAATAATTTCAGAGCAAAACAACCCTCGAGTAGAGGTTTGGCCATCAGATTCAGGGGTCAGAAGTGTGATATCTAGGTTTAGGAGTTTGCCTGGACATGAACAAAGTAAAAAGATGGTGGATCAGGAGGTCTTCTTGAGGCAAAACATTAGGAAAGTCTACGAGCAGTTGAAGAAGCAAAGGGAGGAAACCAGAAAGATGGAAATGACCAATATCATTGATCATTATATTCAAACCATAGAATTTAATGGAAATTCGATGAGCAAATCTGATCTTAATGATTTTTCATCTTTTATTGATGAGAATTTGAAGGAGGTTGATCGGAAGATGAAAGAAATGACAATGAAAGATCAAGAGGAGGTTGGAAATGGGGTGGGATTATGA

Protein sequence

>MS.gene05762.t1

MVRFNLSIISTKHKINEISTLCGVEACAIISEQNNPRVEVWPSDSGVRSVISRFRSLPGHEQSKKMVDQEVFLRQNIRKVYEQLKKQREETRKMEMTNIIDHYIQTIEFNGNSMSKSDLNDFSSFIDENLKEVDRKMKEMTMKDQEEVGNGVGL