AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50068

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50068.t1 MTR_4g069520 68.889 720 163 8 1 663 1 716 0.0 951
MS.gene50068.t1 MTR_4g069520 67.575 734 163 9 1 663 1 730 0.0 942
MS.gene50068.t1 MTR_4g069550 75.276 453 65 8 238 663 38 470 0.0 650
MS.gene50068.t1 MTR_4g069550 86.854 213 22 2 3 215 4 210 3.68e-123 374
MS.gene50068.t1 MTR_4g069570 73.353 334 75 3 334 656 6 336 1.76e-177 508
MS.gene50068.t1 MTR_4g069570 59.140 93 20 1 240 332 6 80 2.94e-24 104
MS.gene50068.t1 MTR_4g069570 56.250 96 22 2 180 264 2 88 2.98e-23 102
MS.gene50068.t1 MTR_4g069570 48.246 114 32 3 104 217 2 88 2.98e-19 90.1
MS.gene50068.t1 MTR_4g069570 50.000 98 31 2 82 179 9 88 7.53e-18 85.9
MS.gene50068.t1 MTR_4g069040 65.781 301 82 4 365 661 232 515 4.39e-139 417
MS.gene50068.t1 MTR_3g116270 38.343 519 262 16 169 658 19 508 5.69e-96 305
MS.gene50068.t1 MTR_6g086530 70.244 205 60 1 452 655 1 205 1.11e-94 291
MS.gene50068.t1 MTR_3g116320 49.669 302 117 7 379 658 402 690 1.38e-91 299
MS.gene50068.t1 MTR_4g069680 47.032 219 111 4 440 655 70 286 2.66e-63 212
MS.gene50068.t1 MTR_4g068420 64.384 146 33 1 518 663 2 128 4.54e-63 205
MS.gene50068.t1 MTR_3g107830 47.345 226 114 4 440 662 39 262 4.73e-63 210
MS.gene50068.t1 MTR_3g116410 46.903 226 115 4 440 662 59 282 6.45e-61 205
MS.gene50068.t1 MTR_3g116380 43.636 220 118 4 440 656 87 303 2.51e-55 191
MS.gene50068.t1 MTR_1g008500 38.813 219 124 6 440 654 171 383 2.44e-41 155
MS.gene50068.t1 MTR_3g109490 38.258 264 142 9 405 655 66 321 3.27e-41 153
MS.gene50068.t1 MTR_1g008470 36.574 216 128 5 440 653 89 297 3.42e-39 146
MS.gene50068.t1 MTR_0312s0030 36.239 218 129 6 440 654 113 323 1.05e-38 146
MS.gene50068.t1 MTR_1g007520 36.239 218 129 6 440 654 113 323 1.05e-38 146
MS.gene50068.t1 MTR_1g008590 36.239 218 129 6 440 654 113 323 3.63e-38 144
MS.gene50068.t1 MTR_1g008460 35.981 214 129 5 440 653 89 294 9.49e-38 142
MS.gene50068.t1 MTR_1g008510 36.574 216 130 5 440 654 115 324 1.00e-37 143
MS.gene50068.t1 MTR_1g008530 34.884 215 129 6 445 654 14 222 2.80e-36 136
MS.gene50068.t1 MTR_1g008560 35.455 220 131 6 440 654 115 328 6.43e-36 138
MS.gene50068.t1 MTR_1g008450 36.316 190 114 4 464 653 128 310 1.33e-32 128
MS.gene50068.t1 MTR_4g069540 69.412 85 26 0 419 503 13 97 2.22e-32 121
MS.gene50068.t1 MTR_1g008580 35.795 176 106 5 480 654 1 170 4.08e-31 120
MS.gene50068.t1 MTR_8g064560 33.505 194 117 5 464 653 438 623 1.05e-26 115
MS.gene50068.t1 MTR_5g034320 31.959 194 120 6 464 653 441 626 1.57e-25 112
MS.gene50068.t1 MTR_3g078090 30.455 220 139 6 440 653 413 624 1.52e-24 108
MS.gene50068.t1 MTR_8g064530 31.959 194 120 6 464 653 455 640 3.68e-24 107
MS.gene50068.t1 MTR_8g064500 31.414 191 125 5 464 653 185 370 5.41e-24 104
MS.gene50068.t1 MTR_4g133840 33.117 154 98 4 501 653 37 186 8.30e-21 91.3
MS.gene50068.t1 MTR_5g033350 28.723 188 121 4 474 653 16 198 1.81e-18 84.7
MS.gene50068.t1 MTR_5g033340 28.723 188 121 4 474 653 195 377 2.20e-16 82.0
MS.gene50068.t1 MTR_5g033340 29.530 149 101 2 485 633 6 150 8.38e-14 73.9
MS.gene50068.t1 MTR_2g081770 32.127 221 116 11 440 655 79 270 1.18e-15 78.2
MS.gene50068.t1 MTR_5g033330 29.167 168 107 3 474 633 226 389 1.62e-14 77.4
MS.gene50068.t1 MTR_5g033330 28.571 168 108 3 474 633 18 181 5.52e-14 75.9
MS.gene50068.t1 MTR_5g033330 27.660 188 123 4 474 653 434 616 1.63e-13 74.3
MS.gene50068.t1 MTR_1g045860 34.000 150 88 6 506 655 121 259 1.97e-13 71.6
MS.gene50068.t1 MTR_2g081610 32.867 143 84 5 513 655 129 259 4.27e-12 67.4
MS.gene50068.t1 MTR_8g046000 30.719 153 90 7 506 656 85 223 2.32e-11 64.7
MS.gene50068.t1 MTR_8g075930 41.176 68 38 2 567 633 11 77 4.89e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50068.t1 AT1G49320 36.486 222 133 3 440 654 58 278 1.49e-47 169
MS.gene50068.t1 AT5G25610 36.073 219 130 6 440 653 176 389 5.41e-38 145
MS.gene50068.t1 AT1G60390 29.897 194 124 6 464 653 435 620 2.08e-23 105
MS.gene50068.t1 AT1G70370 31.282 195 122 7 464 654 437 623 5.89e-23 104
MS.gene50068.t1 AT1G70370 31.282 195 122 7 464 654 437 623 5.89e-23 104
MS.gene50068.t1 AT1G23760 28.351 194 129 6 464 654 433 619 2.29e-20 95.9

Find 223 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCATTGGATTCTGATTGTT+TGG 0.238831 4.2:-37162823 None:intergenic
TTTACAGAAGTACACCATTT+TGG 0.240272 4.2:+37163493 MS.gene50068:CDS
TTGTACCCGGTAGTGTAATC+TGG 0.274373 4.2:-37162744 None:intergenic
ATGTACCCGTTAAGGTAATT+TGG 0.281112 4.2:-37163026 None:intergenic
TCCATTGGATTCTTGTTGTT+TGG 0.293372 4.2:-37162877 None:intergenic
GTGTATGAAGCTGGGTAATT+TGG 0.297089 4.2:-37161994 None:intergenic
TTGAACCCGGTAGTGTAATC+TGG 0.302982 4.2:-37162603 None:intergenic
ACCCAGATCATATTATATTT+AGG 0.313828 4.2:+37163702 MS.gene50068:CDS
AATTTGGTTGCTTGGCTTCA+TGG 0.313905 4.2:-37163010 None:intergenic
ATTTAGGCAGCTTGGCATTA+AGG 0.314472 4.2:+37163718 MS.gene50068:CDS
GGTAGTGTAATCTGGGTTCT+TGG 0.317068 4.2:-37162595 None:intergenic
GGTAGTGTAATCTGGGTTCT+TGG 0.317068 4.2:-37162736 None:intergenic
TGTAGTGTAATCCGGGTTCT+TGG 0.321752 4.2:-37162112 None:intergenic
TGTAGTGTAATCCGGGTTCT+TGG 0.321752 4.2:-37162226 None:intergenic
TGTAGTGTAATCCGGGTTCT+TGG 0.321752 4.2:-37162340 None:intergenic
TGTAGTGTAATCCGGGTTCT+TGG 0.321752 4.2:-37162454 None:intergenic
TTAGCTTGAGGAGAATCTTC+AGG 0.325821 4.2:-37163302 None:intergenic
TGCCTAAATATAATATGATC+TGG 0.343870 4.2:-37163704 None:intergenic
GGGCCTTACATTACTCAATA+TGG 0.344598 4.2:+37162561 MS.gene50068:CDS
CGTACAATTCATTAGATTCC+TGG 0.344740 4.2:-37162971 None:intergenic
TTACCATATTGATTTATATA+AGG 0.347046 4.2:-37162705 None:intergenic
TTGGTTTACTGCCTCCGTAC+TGG 0.347317 4.2:-37161975 None:intergenic
TGATATATGCTTGTTGTGTT+TGG 0.352204 4.2:-37162022 None:intergenic
ATCATGTGATACTTCCAAAA+TGG 0.355384 4.2:-37163507 None:intergenic
TTGTACCCTGTAGTGTAATC+CGG 0.360303 4.2:-37162462 None:intergenic
TTGGTGGTGGTGTATGAAGC+TGG 0.361912 4.2:-37162003 None:intergenic
GGCAGTGTTGTAAGGTTCAT+TGG 0.362108 4.2:-37162784 None:intergenic
TCCATTGGAATCCAGTTGTT+TGG 0.364793 4.2:-37162682 None:intergenic
CCCATTGGAATCCAGCTGTT+TGG 0.367673 4.2:-37162541 None:intergenic
GGGCCTTACATAACTCAATA+TGG 0.370659 4.2:+37162843 MS.gene50068:CDS
TGTGAATCGGAAACCATTAC+AGG 0.374526 4.2:+37163350 MS.gene50068:CDS
AGATCGTTGGAGGTGGTAGA+TGG 0.379193 4.2:-37163077 None:intergenic
GGACCTTATATAAATCAATA+TGG 0.380976 4.2:+37162702 MS.gene50068:CDS
GTGATCCAAGTTAGGAGATT+GGG 0.384612 4.2:-37163108 None:intergenic
TGATGCGAATGGAGATAAAA+TGG 0.384644 4.2:+37163640 MS.gene50068:CDS
GAATTTGTTGACATGATCAT+TGG 0.389245 4.2:+37163413 MS.gene50068:CDS
TTGTACCCTGTAGTGTAATC+CGG 0.392483 4.2:-37162120 None:intergenic
TTGTACCCTGTAGTGTAATC+CGG 0.392483 4.2:-37162234 None:intergenic
TTGTACCCTGTAGTGTAATC+CGG 0.392483 4.2:-37162348 None:intergenic
TTTCATTAGATTCATGGGTA+TGG 0.395484 4.2:-37162146 None:intergenic
TTTCATTAGATTCATGGGTA+TGG 0.395484 4.2:-37162260 None:intergenic
TTTCATTAGATTCATGGGTA+TGG 0.395484 4.2:-37162374 None:intergenic
TATGTAATGACAAAAGTAAA+TGG 0.399290 4.2:-37163579 None:intergenic
GCCATTTGGACACATCTGAT+TGG 0.403046 4.2:+37163678 MS.gene50068:CDS
TGTAAGGTTCAATGGATTCA+TGG 0.403280 4.2:-37162635 None:intergenic
AGCAACCAAATTACCTTAAC+GGG 0.404392 4.2:+37163021 MS.gene50068:CDS
GGCAGTTATGTAAGGTTCAA+TGG 0.409676 4.2:-37162643 None:intergenic
GGATGACAAGCTACCCATTT+GGG 0.410770 4.2:-37163539 None:intergenic
TGTAAGGTCCACTGGATTCA+TGG 0.411259 4.2:-37162494 None:intergenic
TTCATTAGATTCATGGGTAT+GGG 0.412584 4.2:-37162145 None:intergenic
TTCATTAGATTCATGGGTAT+GGG 0.412584 4.2:-37162259 None:intergenic
TTCATTAGATTCATGGGTAT+GGG 0.412584 4.2:-37162373 None:intergenic
AGCCGTTGGTGAGGGTTTGT+TGG 0.413150 4.2:-37163465 None:intergenic
AGCTGTTGGAATTTGCCATT+TGG 0.418321 4.2:+37163664 MS.gene50068:CDS
CAACAGATATGTACCCGTTA+AGG 0.419009 4.2:-37163034 None:intergenic
CAGCTCTACCTTCAAGTCTC+TGG 0.419943 4.2:-37161908 None:intergenic
TGACAAAAGTAAATGGCATA+TGG 0.422075 4.2:-37163572 None:intergenic
CTCTCCAAGTGAATTTATCA+TGG 0.423407 4.2:-37163327 None:intergenic
GTTAAGGTAATTTGGTTGCT+TGG 0.424962 4.2:-37163018 None:intergenic
GTTCATTGGATGCATGGGTA+TGG 0.426590 4.2:-37162770 None:intergenic
GTCCACTGGATTCATGGGTA+TGG 0.427423 4.2:-37162488 None:intergenic
GGATCACACAGAAGCTTTCA+AGG 0.433713 4.2:+37163124 MS.gene50068:CDS
AGTCTTTGTCTCCCCTGTAA+TGG 0.434357 4.2:-37163363 None:intergenic
TACCTGTGAACCATCTTATG+TGG 0.435229 4.2:+37163771 MS.gene50068:CDS
AATTTGGATGATCTTTATGT+TGG 0.440932 4.2:+37163158 MS.gene50068:CDS
TTACCATATTGAGTTATGTA+AGG 0.441136 4.2:-37162846 None:intergenic
GCTTCTGTGTGATCCAAGTT+AGG 0.443750 4.2:-37163116 None:intergenic
GAACCTTACATAACTGCCTT+CGG 0.444477 4.2:+37162648 MS.gene50068:CDS
GTTCAATGGATTCATGGGTA+TGG 0.445281 4.2:-37162629 None:intergenic
CATATTATATTTAGGCAGCT+TGG 0.445595 4.2:+37163710 MS.gene50068:CDS
TTGATTTATATAAGGTCCAT+TGG 0.446324 4.2:-37162697 None:intergenic
TGGATGACAAGCTACCCATT+TGG 0.450677 4.2:-37163540 None:intergenic
AAATGGTGTACTTCTGTAAA+GGG 0.451382 4.2:-37163490 None:intergenic
ATGCTTGTTGTGTTTGGTGG+TGG 0.454019 4.2:-37162016 None:intergenic
AAGCAACCAAATTACCTTAA+CGG 0.454443 4.2:+37163020 MS.gene50068:CDS
AAAATGGTGTACTTCTGTAA+AGG 0.455484 4.2:-37163491 None:intergenic
CATCAATGCCAGAGACTTGA+AGG 0.456451 4.2:+37161900 MS.gene50068:CDS
TTACCATAGGCAGTGTTGTA+AGG 0.456880 4.2:-37162792 None:intergenic
TATATGCTTGTTGTGTTTGG+TGG 0.457052 4.2:-37162019 None:intergenic
TGGACATGAAGCCAAACAAC+TGG 0.458874 4.2:+37162671 MS.gene50068:CDS
ATGATATTGATGCTCCCAAA+TGG 0.459757 4.2:+37163525 MS.gene50068:CDS
GAACCTTACAACACTGCCTA+TGG 0.460056 4.2:+37162789 MS.gene50068:CDS
TACAGGGACTAGAGTGTTCA+AGG 0.461314 4.2:+37163604 MS.gene50068:CDS
GGAAGTTGTGTAAGGTCCAT+TGG 0.462228 4.2:-37162892 None:intergenic
TTGAGTTATGTAAGGCCCAT+TGG 0.465944 4.2:-37162838 None:intergenic
TTACCATATTGAGTAATGTA+AGG 0.470153 4.2:-37162564 None:intergenic
GAAGGTAGAGCTGCAAGATC+AGG 0.475278 4.2:+37161918 MS.gene50068:CDS
TTACCATAGGAAGTTGTGTA+AGG 0.475470 4.2:-37162900 None:intergenic
ACCACCTCCAACGATCTAGA+AGG 0.478247 4.2:+37163083 MS.gene50068:CDS
TGGTTTACTGCCTCCGTACT+GGG 0.478278 4.2:-37161974 None:intergenic
GATCGTTGGAGGTGGTAGAT+GGG 0.479040 4.2:-37163076 None:intergenic
TAGCTTGAGGAGAATCTTCA+GGG 0.480432 4.2:-37163301 None:intergenic
CTGTACCCGCCAGTCATGTA+AGG 0.481597 4.2:-37162066 None:intergenic
GGGGCCTTCTAGATCGTTGG+AGG 0.482641 4.2:-37163087 None:intergenic
CTGTAACCGCCAGTCATGTA+AGG 0.482850 4.2:-37162180 None:intergenic
CTGTACCCGCCAGTCATGTA+AGG 0.482850 4.2:-37162294 None:intergenic
CTGTACCCGCCAGTCATGTA+AGG 0.482850 4.2:-37162408 None:intergenic
GAATTGTACGTATCTGCATA+TGG 0.484602 4.2:+37162984 MS.gene50068:CDS
GCATTGGAACCCACATAAGA+TGG 0.485432 4.2:-37163781 None:intergenic
GTGTTCAAGGTCTCACTTGT+CGG 0.486727 4.2:+37163617 MS.gene50068:CDS
CCACCGAAGGCAGTTATGTA+AGG 0.487517 4.2:-37162651 None:intergenic
GGACCTTACACAACTTCCTA+TGG 0.490690 4.2:+37162897 MS.gene50068:CDS
TGTGATCCAAGTTAGGAGAT+TGG 0.490761 4.2:-37163109 None:intergenic
TCCACTGGATTCATGGGTAT+GGG 0.493934 4.2:-37162487 None:intergenic
GGAAGTTATGTAAGGTCCAC+TGG 0.495055 4.2:-37162502 None:intergenic
GGTGGTGAGAATACTATGTT+TGG 0.495632 4.2:-37163444 None:intergenic
TGTACCCTGTAGTGTAATCC+GGG 0.499981 4.2:-37162461 None:intergenic
CTTTGCTTCTTGTTTACCAT+AGG 0.500812 4.2:-37162913 None:intergenic
GAGAAGAGTTGAAGAACACT+TGG 0.501370 4.2:-37163275 None:intergenic
TTGAGTAATGTAAGGCCCAT+TGG 0.501940 4.2:-37162556 None:intergenic
TTTATCATGGATTTAGCTTG+AGG 0.502246 4.2:-37163314 None:intergenic
TCCAATCAGATGTGTCCAAA+TGG 0.503560 4.2:-37163679 None:intergenic
CCACTTGGACGGGAAATTGA+AGG 0.503790 4.2:-37163193 None:intergenic
CTAAGCGAGCCTTACATGAC+TGG 0.509211 4.2:+37162171 MS.gene50068:CDS
CTAAGCGAGCCTTACATGAC+TGG 0.509211 4.2:+37162285 MS.gene50068:CDS
CTAAGCGAGCCTTACATGAC+TGG 0.509211 4.2:+37162399 MS.gene50068:CDS
ATCGTTGGAGGTGGTAGATG+GGG 0.509770 4.2:-37163075 None:intergenic
AAGAACCCGGATTACACTAC+AGG 0.510637 4.2:+37162114 MS.gene50068:CDS
AAGAACCCGGATTACACTAC+AGG 0.510637 4.2:+37162228 MS.gene50068:CDS
AAGAACCCGGATTACACTAC+AGG 0.510637 4.2:+37162342 MS.gene50068:CDS
AAGAACCCGGATTACACTAC+AGG 0.510637 4.2:+37162456 MS.gene50068:CDS
GCCTTCTAGATCGTTGGAGG+TGG 0.512982 4.2:-37163084 None:intergenic
CCTCCGTACTGGGTAATGTA+TGG 0.514085 4.2:-37161964 None:intergenic
AGGCAGCTTGGCATTAAGGC+CGG 0.516232 4.2:+37163722 MS.gene50068:CDS
CGTTGGTGAGGGTTTGTTGG+TGG 0.516901 4.2:-37163462 None:intergenic
ACCTGTGAACCATCTTATGT+GGG 0.517632 4.2:+37163772 MS.gene50068:CDS
GGAGATAAAATGGAAGCTGT+TGG 0.517741 4.2:+37163650 MS.gene50068:CDS
TTCATTGGATGCATGGGTAT+GGG 0.519593 4.2:-37162769 None:intergenic
TTCACTTGGAGAGTGTGAAT+CGG 0.519976 4.2:+37163337 MS.gene50068:CDS
GTAAGGTTCAATGGATTCAT+GGG 0.520886 4.2:-37162634 None:intergenic
TGGTGGTGGTGTATGAAGCT+GGG 0.523140 4.2:-37162002 None:intergenic
GCCTAAATATAATATGATCT+GGG 0.525219 4.2:-37163703 None:intergenic
GTACAATTCATTAGATTCCT+GGG 0.527136 4.2:-37162970 None:intergenic
CTGGGTATGAGTGTTATATC+CGG 0.528373 4.2:-37162952 None:intergenic
TTCAATGGATTCATGGGTAT+GGG 0.529636 4.2:-37162628 None:intergenic
TGTACCCGGTAGTGTAATCT+GGG 0.532124 4.2:-37162743 None:intergenic
TTTGGCTTCATGTTTACCAT+AGG 0.532676 4.2:-37162805 None:intergenic
TGAACCCGGTAGTGTAATCT+GGG 0.534636 4.2:-37162602 None:intergenic
CTACCGTAGGAAGTTATGTA+AGG 0.538633 4.2:-37162510 None:intergenic
GGCAGCTTGGCATTAAGGCC+GGG 0.541302 4.2:+37163723 MS.gene50068:CDS
TCTGTTGGAAATGACCCAAA+AGG 0.545209 4.2:+37163050 MS.gene50068:CDS
ACACACTGGAGAGTTCTTCC+CGG 0.545365 4.2:-37163741 None:intergenic
CTTAACGGGTACATATCTGT+TGG 0.545684 4.2:+37163035 MS.gene50068:CDS
TGATATTGATGCTCCCAAAT+GGG 0.545823 4.2:+37163526 MS.gene50068:CDS
TCGTTGGAGGTGGTAGATGG+GGG 0.546821 4.2:-37163074 None:intergenic
GGCGGTTACAGACTTGCATA+TGG 0.547871 4.2:+37162192 MS.gene50068:CDS
GTAAGGTCCACTGGATTCAT+GGG 0.548211 4.2:-37162493 None:intergenic
CCAAACAATCAGAATCCAAT+GGG 0.549989 4.2:+37162823 MS.gene50068:CDS
AAGAACCCAGATTACACTAC+CGG 0.550854 4.2:+37162597 MS.gene50068:CDS
TGTACCCTGTAGTGTAATCC+GGG 0.551924 4.2:-37162119 None:intergenic
TGTACCCTGTAGTGTAATCC+GGG 0.551924 4.2:-37162233 None:intergenic
TGTACCCTGTAGTGTAATCC+GGG 0.551924 4.2:-37162347 None:intergenic
AAGAACCCAGATTACACTAC+CGG 0.556042 4.2:+37162738 MS.gene50068:CDS
GGCGGGTACAGACTTGCATA+TGG 0.556538 4.2:+37162078 MS.gene50068:CDS
GGCGGGTACAGACTTGCATA+TGG 0.556538 4.2:+37162306 MS.gene50068:CDS
GGCGGGTACAGACTTGCATA+TGG 0.556538 4.2:+37162420 MS.gene50068:CDS
GCCAAACAGCTGGATTCCAA+TGG 0.556982 4.2:+37162540 MS.gene50068:CDS
TAGACATGAAGCCAAACAGC+TGG 0.557804 4.2:+37162530 MS.gene50068:CDS
AAAGGAGAAACCACTTGGAC+GGG 0.559421 4.2:-37163203 None:intergenic
TGTAAGGTTCATTGGATGCA+TGG 0.559989 4.2:-37162776 None:intergenic
TTGTGACATTGCAAGAGGAA+TGG 0.560283 4.2:-37163249 None:intergenic
CCAAACAGCTGGATTCCAAT+GGG 0.560358 4.2:+37162541 MS.gene50068:CDS
AGAGGAAAGGAGAAACCACT+TGG 0.562679 4.2:-37163208 None:intergenic
CTAAGCGATCCTTACATGAC+TGG 0.562821 4.2:+37162057 MS.gene50068:CDS
CACACAGAAGCTTTCAAGGT+AGG 0.566929 4.2:+37163128 MS.gene50068:CDS
AAGCAACAAAGTTACAATAC+CGG 0.568840 4.2:+37162933 MS.gene50068:CDS
TCACTTGTCGGTGATGCGAA+TGG 0.570327 4.2:+37163629 MS.gene50068:CDS
TAAAGGGACAGCCGTTGGTG+AGG 0.576755 4.2:-37163474 None:intergenic
TTTGGCTTCATGTCCACCGA+AGG 0.576894 4.2:-37162664 None:intergenic
AAACCATACATTACCCAGTA+CGG 0.577354 4.2:+37161961 MS.gene50068:CDS
ACCCACATAAGATGGTTCAC+AGG 0.577363 4.2:-37163773 None:intergenic
ATGGGTATGGGTCTTGAACC+CGG 0.577717 4.2:-37162616 None:intergenic
ATGGGTATGGGTCTTGTACC+CGG 0.577866 4.2:-37162757 None:intergenic
CGGATATAACACTCATACCC+AGG 0.579747 4.2:+37162953 MS.gene50068:CDS
AAAGGGACAGCCGTTGGTGA+GGG 0.580139 4.2:-37163473 None:intergenic
GCCAAACAATCAGAATCCAA+TGG 0.580573 4.2:+37162822 MS.gene50068:CDS
GAGCATGGACTCTAGAGAGT+TGG 0.580577 4.2:-37163390 None:intergenic
TGATCCAAGTTAGGAGATTG+GGG 0.581553 4.2:-37163107 None:intergenic
TTTGGCTTCATGTCTACCGT+AGG 0.582386 4.2:-37162523 None:intergenic
GTAAGGTTCATTGGATGCAT+GGG 0.583311 4.2:-37162775 None:intergenic
CCTTCAATTTCCCGTCCAAG+TGG 0.588140 4.2:+37163193 MS.gene50068:CDS
TTCTGTAAAGGGACAGCCGT+TGG 0.589659 4.2:-37163479 None:intergenic
TAAACATGAAGCCAAGAACC+CGG 0.590476 4.2:+37162101 MS.gene50068:CDS
TAAACATGAAGCCAAGAACC+CGG 0.590476 4.2:+37162215 MS.gene50068:CDS
TAAACATGAAGCCAAGAACC+CGG 0.590476 4.2:+37162329 MS.gene50068:CDS
TAAACATGAAGCCAAGAACC+CGG 0.590476 4.2:+37162443 MS.gene50068:CDS
GGACCTTACATAACTTCCTA+CGG 0.597436 4.2:+37162507 MS.gene50068:CDS
GAAAGGAGAAACCACTTGGA+CGG 0.598116 4.2:-37163204 None:intergenic
TTTGTCATTACATAGCTACA+GGG 0.598347 4.2:+37163588 MS.gene50068:CDS
CACTTGGACGGGAAATTGAA+GGG 0.598885 4.2:-37163192 None:intergenic
CCTTACATAACTGCCTTCGG+TGG 0.600129 4.2:+37162651 MS.gene50068:CDS
GCGATCCTTACATGACTGGC+GGG 0.601793 4.2:+37162061 MS.gene50068:CDS
AAATCCATGATAAATTCACT+TGG 0.603290 4.2:+37163323 MS.gene50068:CDS
CACCAACAAACCCTCACCAA+CGG 0.604728 4.2:+37163463 MS.gene50068:CDS
GCCAAACAACTGGATTCCAA+TGG 0.609705 4.2:+37162681 MS.gene50068:CDS
GGGAGTTGTGACATTGCAAG+AGG 0.609821 4.2:-37163254 None:intergenic
GCTGCAAGATCAGGATCAAG+TGG 0.609867 4.2:+37161927 MS.gene50068:CDS
ATGGCATATGGATATGAGAG+TGG 0.619183 4.2:-37163560 None:intergenic
AGCGATCCTTACATGACTGG+CGG 0.623067 4.2:+37162060 MS.gene50068:CDS
CATGTCAACAAATTCGAGCA+TGG 0.623516 4.2:-37163405 None:intergenic
CGTTGGAGGTGGTAGATGGG+GGG 0.625306 4.2:-37163073 None:intergenic
GCCAAACAACAAGAATCCAA+TGG 0.625758 4.2:+37162876 MS.gene50068:CDS
GCGAGCCTTACATGACTGGC+GGG 0.626816 4.2:+37162289 MS.gene50068:CDS
GCGAGCCTTACATGACTGGC+GGG 0.626816 4.2:+37162403 MS.gene50068:CDS
GTGAATCGGAAACCATTACA+GGG 0.628683 4.2:+37163351 MS.gene50068:CDS
AGAAGAGTTGAAGAACACTT+GGG 0.631035 4.2:-37163274 None:intergenic
AGCGAGCCTTACATGACTGG+CGG 0.648735 4.2:+37162288 MS.gene50068:CDS
AGCGAGCCTTACATGACTGG+CGG 0.648735 4.2:+37162402 MS.gene50068:CDS
ACCCATACCCATGAATCCAG+TGG 0.649681 4.2:+37162486 MS.gene50068:CDS
AGAACCCAGATTACACTACC+GGG 0.654066 4.2:+37162598 MS.gene50068:CDS
ACAGGTAAGAAGTGACACAC+TGG 0.654861 4.2:-37163755 None:intergenic
ATAGACGAACTCACTTCACA+TGG 0.657262 4.2:-37163819 None:intergenic
AGAACCCAGATTACACTACC+GGG 0.659254 4.2:+37162739 MS.gene50068:CDS
AGAACCCGGATTACACTACA+GGG 0.688478 4.2:+37162115 MS.gene50068:CDS
AGAACCCGGATTACACTACA+GGG 0.688478 4.2:+37162229 MS.gene50068:CDS
AGAACCCGGATTACACTACA+GGG 0.688478 4.2:+37162343 MS.gene50068:CDS
AGAACCCGGATTACACTACA+GGG 0.688478 4.2:+37162457 MS.gene50068:CDS
GACGAACTCACTTCACATGG+AGG 0.694583 4.2:-37163816 None:intergenic
AGCGAGCCTTACATGACTGG+CGG 0.703664 4.2:+37162174 MS.gene50068:CDS
CCATACATTACCCAGTACGG+AGG 0.735732 4.2:+37161964 MS.gene50068:CDS
GATCCAAGTTAGGAGATTGG+GGG 0.743862 4.2:-37163106 None:intergenic
TGAATCGGAAACCATTACAG+GGG 0.744089 4.2:+37163352 MS.gene50068:CDS
AGAAGAAAAGAACTACTGTG+AGG 0.750096 4.2:-37160030 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAAAATAAAAAAAA+TGG - chr4.2:37160598-37160617 None:intergenic 0.0%
!! TAAATAAAAATAAAAAAAAT+GGG - chr4.2:37160597-37160616 None:intergenic 0.0%
!!! TTATAATTATAAATTTTTTA+AGG + chr4.2:37160380-37160399 MS.gene50068:intron 0.0%
!! TATATTAAACTTAACTAATA+AGG - chr4.2:37161475-37161494 None:intergenic 10.0%
!! TATATTGAAAGAAAAATTAT+AGG - chr4.2:37160489-37160508 None:intergenic 10.0%
!!! AATGTAAATTTTTATTTTCA+AGG - chr4.2:37160332-37160351 None:intergenic 10.0%
!!! ATTATTTATTTTTTGGTTAA+AGG + chr4.2:37161715-37161734 MS.gene50068:intron 10.0%
!!! TATTCTTTTTTTTTTTTTGA+CGG + chr4.2:37161799-37161818 MS.gene50068:intron 10.0%
!! AAACAAACTAATTGTATATT+TGG - chr4.2:37161325-37161344 None:intergenic 15.0%
!! ATGATAAGTAAAACAATAAA+TGG - chr4.2:37160866-37160885 None:intergenic 15.0%
!! CAATAAATGATTATATAGAA+TGG - chr4.2:37161564-37161583 None:intergenic 15.0%
!! TGATAAGTAAAACAATAAAT+GGG - chr4.2:37160865-37160884 None:intergenic 15.0%
!! TGTTTAGTACAAATTAATTA+TGG + chr4.2:37161340-37161359 MS.gene50068:intron 15.0%
!! TTTATTTACATTTGAATAGA+AGG + chr4.2:37160781-37160800 MS.gene50068:intron 15.0%
!! TTTGATTAAGTTATTAGATA+CGG + chr4.2:37160131-37160150 MS.gene50068:intron 15.0%
!!! ATTCTTTTTTTTTTTTTGAC+GGG + chr4.2:37161800-37161819 MS.gene50068:intron 15.0%
!!! TTACCATATTGATTTATATA+AGG - chr4.2:37162708-37162727 None:intergenic 15.0%
!! TATGTAATGACAAAAGTAAA+TGG - chr4.2:37163582-37163601 None:intergenic 20.0%
!! TGTAAATAAAACTAAAGTGT+CGG - chr4.2:37160771-37160790 None:intergenic 20.0%
!! TTTGTTGAATATTGTGATTA+TGG + chr4.2:37160556-37160575 MS.gene50068:intron 20.0%
!!! ACCTTTCTAAATAGTATTTT+CGG + chr4.2:37161397-37161416 MS.gene50068:intron 20.0%
! AAATCCATGATAAATTCACT+TGG + chr4.2:37163323-37163342 MS.gene50068:CDS 25.0%
! AAGCAATAGTAATATCAACT+TGG + chr4.2:37161757-37161776 MS.gene50068:intron 25.0%
! AAGTAAAACAATAAATGGGA+TGG - chr4.2:37160861-37160880 None:intergenic 25.0%
! AATTGCACATAGTGATATTT+GGG + chr4.2:37160065-37160084 MS.gene50068:intron 25.0%
! AATTTGGATGATCTTTATGT+TGG + chr4.2:37163158-37163177 MS.gene50068:CDS 25.0%
! ACCCAGATCATATTATATTT+AGG + chr4.2:37163702-37163721 MS.gene50068:CDS 25.0%
! ACCGAAAATACTATTTAGAA+AGG - chr4.2:37161401-37161420 None:intergenic 25.0%
! ATAAATTTAGTCGTCATGTT+TGG + chr4.2:37161003-37161022 MS.gene50068:intron 25.0%
! CTAAATCTACAAGATATAGA+TGG - chr4.2:37160647-37160666 None:intergenic 25.0%
! GCCTAAATATAATATGATCT+GGG - chr4.2:37163706-37163725 None:intergenic 25.0%
! GGACCTTATATAAATCAATA+TGG + chr4.2:37162702-37162721 MS.gene50068:CDS 25.0%
! TGCCTAAATATAATATGATC+TGG - chr4.2:37163707-37163726 None:intergenic 25.0%
! TTACCATATTGAGTAATGTA+AGG - chr4.2:37162567-37162586 None:intergenic 25.0%
! TTACCATATTGAGTTATGTA+AGG - chr4.2:37162849-37162868 None:intergenic 25.0%
! TTGATTTATATAAGGTCCAT+TGG - chr4.2:37162700-37162719 None:intergenic 25.0%
!! AGTAGTTCTTTTCTTCTTTA+TGG + chr4.2:37160036-37160055 MS.gene50068:CDS 25.0%
!! TAGCTTTTCATTAGATTCAT+GGG - chr4.2:37162154-37162173 None:intergenic 25.0%
!!! TTAGCTTTTCATTAGATTCA+TGG - chr4.2:37162155-37162174 None:intergenic 25.0%
AAAATGGTGTACTTCTGTAA+AGG - chr4.2:37163494-37163513 None:intergenic 30.0%
AAATGGTGTACTTCTGTAAA+GGG - chr4.2:37163493-37163512 None:intergenic 30.0%
AAGCAACAAAGTTACAATAC+CGG + chr4.2:37162933-37162952 MS.gene50068:CDS 30.0%
AAGCAACCAAATTACCTTAA+CGG + chr4.2:37163020-37163039 MS.gene50068:CDS 30.0%
ATCATGTGATACTTCCAAAA+TGG - chr4.2:37163510-37163529 None:intergenic 30.0%
ATTAGATACGGAGAAAAAAG+AGG + chr4.2:37160143-37160162 MS.gene50068:intron 30.0%
ATTGCACATAGTGATATTTG+GGG + chr4.2:37160066-37160085 MS.gene50068:intron 30.0%
CAATTGCACATAGTGATATT+TGG + chr4.2:37160064-37160083 MS.gene50068:intron 30.0%
CATATTATATTTAGGCAGCT+TGG + chr4.2:37163710-37163729 MS.gene50068:CDS 30.0%
CCAAACAATGCAATTTCTAT+AGG + chr4.2:37160303-37160322 MS.gene50068:intron 30.0%
CCTATAGAAATTGCATTGTT+TGG - chr4.2:37160306-37160325 None:intergenic 30.0%
GTACAATTCATTAGATTCCT+GGG - chr4.2:37162973-37162992 None:intergenic 30.0%
TCTGATTAAAAGTGACTTGA+AGG - chr4.2:37161250-37161269 None:intergenic 30.0%
TGACAAAAGTAAATGGCATA+TGG - chr4.2:37163575-37163594 None:intergenic 30.0%
TTAGATACGGAGAAAAAAGA+GGG + chr4.2:37160144-37160163 MS.gene50068:intron 30.0%
TTCATTAGATTCATGGGTAT+GGG - chr4.2:37162148-37162167 None:intergenic 30.0%
TTGAGTCACACAATTAAATG+TGG - chr4.2:37160521-37160540 None:intergenic 30.0%
TTTCATTAGATTCATGGGTA+TGG - chr4.2:37162149-37162168 None:intergenic 30.0%
TTTGTCATTACATAGCTACA+GGG + chr4.2:37163588-37163607 MS.gene50068:CDS 30.0%
! AGTTAGAGGAAATTTTACGA+GGG - chr4.2:37161538-37161557 None:intergenic 30.0%
! TTTTGTCATTACATAGCTAC+AGG + chr4.2:37163587-37163606 MS.gene50068:CDS 30.0%
!! CAAGGTAGGATTTTTCAATT+TGG + chr4.2:37163142-37163161 MS.gene50068:CDS 30.0%
!! GAATTTGTTGACATGATCAT+TGG + chr4.2:37163413-37163432 MS.gene50068:CDS 30.0%
!! TGATATATGCTTGTTGTGTT+TGG - chr4.2:37162025-37162044 None:intergenic 30.0%
!! TGCATAAAGCTTTGTTAACA+AGG + chr4.2:37161050-37161069 MS.gene50068:intron 30.0%
!! TTTACAGAAGTACACCATTT+TGG + chr4.2:37163493-37163512 MS.gene50068:CDS 30.0%
!! TTTATCATGGATTTAGCTTG+AGG - chr4.2:37163317-37163336 None:intergenic 30.0%
AAACCATACATTACCCAGTA+CGG + chr4.2:37161961-37161980 MS.gene50068:CDS 35.0%
AACAATAAATGGGATGGCTA+AGG - chr4.2:37160855-37160874 None:intergenic 35.0%
AATCTACAATGACAACAACG+AGG - chr4.2:37161872-37161891 None:intergenic 35.0%
ACATTTGAATAGAAGGCAAG+CGG + chr4.2:37160788-37160807 MS.gene50068:intron 35.0%
AGAAGAAAAGAACTACTGTG+AGG - chr4.2:37160033-37160052 None:intergenic 35.0%
AGAAGAGTTGAAGAACACTT+GGG - chr4.2:37163277-37163296 None:intergenic 35.0%
AGCAACCAAATTACCTTAAC+GGG + chr4.2:37163021-37163040 MS.gene50068:CDS 35.0%
ATATAGAATGGCAGAGTTAG+AGG - chr4.2:37161552-37161571 None:intergenic 35.0%
ATGATATTGATGCTCCCAAA+TGG + chr4.2:37163525-37163544 MS.gene50068:CDS 35.0%
ATGTACCCGTTAAGGTAATT+TGG - chr4.2:37163029-37163048 None:intergenic 35.0%
CCAAACAATCAGAATCCAAT+GGG + chr4.2:37162823-37162842 MS.gene50068:CDS 35.0%
CGTACAATTCATTAGATTCC+TGG - chr4.2:37162974-37162993 None:intergenic 35.0%
CTCTCCAAGTGAATTTATCA+TGG - chr4.2:37163330-37163349 None:intergenic 35.0%
GAATTGTACGTATCTGCATA+TGG + chr4.2:37162984-37163003 MS.gene50068:CDS 35.0%
GTTAAGGTAATTTGGTTGCT+TGG - chr4.2:37163021-37163040 None:intergenic 35.0%
TAAAAAAAATGGGGTGACCA+AGG - chr4.2:37160587-37160606 None:intergenic 35.0%
TATCAACTTGGCTAATCACT+TGG + chr4.2:37161769-37161788 MS.gene50068:intron 35.0%
TCCTTTCCTCTCAAAAAAAG+AGG + chr4.2:37163220-37163239 MS.gene50068:CDS 35.0%
TCGTTGTTGTCATTGTAGAT+TGG + chr4.2:37161871-37161890 MS.gene50068:intron 35.0%
TGATATTGATGCTCCCAAAT+GGG + chr4.2:37163526-37163545 MS.gene50068:CDS 35.0%
TGATGCGAATGGAGATAAAA+TGG + chr4.2:37163640-37163659 MS.gene50068:CDS 35.0%
TGGTTCATTACAGGAAAATG+AGG - chr4.2:37160286-37160305 None:intergenic 35.0%
TTACCATAGGAAGTTGTGTA+AGG - chr4.2:37162903-37162922 None:intergenic 35.0%
! CTTTGCTTCTTGTTTACCAT+AGG - chr4.2:37162916-37162935 None:intergenic 35.0%
! GAGTTAGAGGAAATTTTACG+AGG - chr4.2:37161539-37161558 None:intergenic 35.0%
! GTAAGGTTCAATGGATTCAT+GGG - chr4.2:37162637-37162656 None:intergenic 35.0%
! TGCATTGTTTGGTTCATTAC+AGG - chr4.2:37160295-37160314 None:intergenic 35.0%
! TGTAAGGTTCAATGGATTCA+TGG - chr4.2:37162638-37162657 None:intergenic 35.0%
! TTCAATGGATTCATGGGTAT+GGG - chr4.2:37162631-37162650 None:intergenic 35.0%
! TTTGGCTTCATGTTTACCAT+AGG - chr4.2:37162808-37162827 None:intergenic 35.0%
!! TATATGCTTGTTGTGTTTGG+TGG - chr4.2:37162022-37162041 None:intergenic 35.0%
!! TCCATTGGATTCTTGTTGTT+TGG - chr4.2:37162880-37162899 None:intergenic 35.0%
AAGAACCCAGATTACACTAC+CGG + chr4.2:37162597-37162616 MS.gene50068:CDS 40.0%
AATTTGGTTGCTTGGCTTCA+TGG - chr4.2:37163013-37163032 None:intergenic 40.0%
ACCTGTGAACCATCTTATGT+GGG + chr4.2:37163772-37163791 MS.gene50068:CDS 40.0%
AGCTGTTGGAATTTGCCATT+TGG + chr4.2:37163664-37163683 MS.gene50068:CDS 40.0%
ATGGCATATGGATATGAGAG+TGG - chr4.2:37163563-37163582 None:intergenic 40.0%
ATTTAGGCAGCTTGGCATTA+AGG + chr4.2:37163718-37163737 MS.gene50068:CDS 40.0%
CAACAGATATGTACCCGTTA+AGG - chr4.2:37163037-37163056 None:intergenic 40.0%
CATGTCAACAAATTCGAGCA+TGG - chr4.2:37163408-37163427 None:intergenic 40.0%
CATTTGAATAGAAGGCAAGC+GGG + chr4.2:37160789-37160808 MS.gene50068:intron 40.0%
CCCATTGGATTCTGATTGTT+TGG - chr4.2:37162826-37162845 None:intergenic 40.0%
CTACCGTAGGAAGTTATGTA+AGG - chr4.2:37162513-37162532 None:intergenic 40.0%
CTGGGTATGAGTGTTATATC+CGG - chr4.2:37162955-37162974 None:intergenic 40.0%
GAACCTTACATAACTGCCTT+CGG + chr4.2:37162648-37162667 MS.gene50068:CDS 40.0%
GAGAAGAGTTGAAGAACACT+TGG - chr4.2:37163278-37163297 None:intergenic 40.0%
GCCAAACAACAAGAATCCAA+TGG + chr4.2:37162876-37162895 MS.gene50068:CDS 40.0%
GCCAAACAATCAGAATCCAA+TGG + chr4.2:37162822-37162841 MS.gene50068:CDS 40.0%
GGACCTTACATAACTTCCTA+CGG + chr4.2:37162507-37162526 MS.gene50068:CDS 40.0%
GGAGATAAAATGGAAGCTGT+TGG + chr4.2:37163650-37163669 MS.gene50068:CDS 40.0%
GGCAGTTATGTAAGGTTCAA+TGG - chr4.2:37162646-37162665 None:intergenic 40.0%
GGGCCTTACATAACTCAATA+TGG + chr4.2:37162843-37162862 MS.gene50068:CDS 40.0%
GGGCCTTACATTACTCAATA+TGG + chr4.2:37162561-37162580 MS.gene50068:CDS 40.0%
GGTGGTGAGAATACTATGTT+TGG - chr4.2:37163447-37163466 None:intergenic 40.0%
GTAAGGTTCATTGGATGCAT+GGG - chr4.2:37162778-37162797 None:intergenic 40.0%
GTGAATCGGAAACCATTACA+GGG + chr4.2:37163351-37163370 MS.gene50068:CDS 40.0%
GTGATCCAAGTTAGGAGATT+GGG - chr4.2:37163111-37163130 None:intergenic 40.0%
GTGTATGAAGCTGGGTAATT+TGG - chr4.2:37161997-37162016 None:intergenic 40.0%
TAAACATGAAGCCAAGAACC+CGG + chr4.2:37162101-37162120 MS.gene50068:CDS 40.0%
TACCTGTGAACCATCTTATG+TGG + chr4.2:37163771-37163790 MS.gene50068:CDS 40.0%
TAGCTTGAGGAGAATCTTCA+GGG - chr4.2:37163304-37163323 None:intergenic 40.0%
TCCAATCAGATGTGTCCAAA+TGG - chr4.2:37163682-37163701 None:intergenic 40.0%
TCCATTGGAATCCAGTTGTT+TGG - chr4.2:37162685-37162704 None:intergenic 40.0%
TCTGTTGGAAATGACCCAAA+AGG + chr4.2:37163050-37163069 MS.gene50068:CDS 40.0%
TGAATCGGAAACCATTACAG+GGG + chr4.2:37163352-37163371 MS.gene50068:CDS 40.0%
TGATCCAAGTTAGGAGATTG+GGG - chr4.2:37163110-37163129 None:intergenic 40.0%
TGTAAGGTTCATTGGATGCA+TGG - chr4.2:37162779-37162798 None:intergenic 40.0%
TGTGAATCGGAAACCATTAC+AGG + chr4.2:37163350-37163369 MS.gene50068:CDS 40.0%
TGTGATCCAAGTTAGGAGAT+TGG - chr4.2:37163112-37163131 None:intergenic 40.0%
TTACCATAGGCAGTGTTGTA+AGG - chr4.2:37162795-37162814 None:intergenic 40.0%
TTCATTGGATGCATGGGTAT+GGG - chr4.2:37162772-37162791 None:intergenic 40.0%
TTGAGTAATGTAAGGCCCAT+TGG - chr4.2:37162559-37162578 None:intergenic 40.0%
TTGAGTTATGTAAGGCCCAT+TGG - chr4.2:37162841-37162860 None:intergenic 40.0%
TTGTACCCTGTAGTGTAATC+CGG - chr4.2:37162123-37162142 None:intergenic 40.0%
TTGTGACATTGCAAGAGGAA+TGG - chr4.2:37163252-37163271 None:intergenic 40.0%
TTGTGATTATGGTGTAGCCT+TGG + chr4.2:37160567-37160586 MS.gene50068:intron 40.0%
! CATAGTGATATTTGGGGTCT+TGG + chr4.2:37160072-37160091 MS.gene50068:intron 40.0%
! CTTAACGGGTACATATCTGT+TGG + chr4.2:37163035-37163054 MS.gene50068:CDS 40.0%
! GTTCAATGGATTCATGGGTA+TGG - chr4.2:37162632-37162651 None:intergenic 40.0%
! TTAGCTTGAGGAGAATCTTC+AGG - chr4.2:37163305-37163324 None:intergenic 40.0%
! TTCACTTGGAGAGTGTGAAT+CGG + chr4.2:37163337-37163356 MS.gene50068:CDS 40.0%
!!! AGTCAGCCTCTTTTTTTGAG+AGG - chr4.2:37163229-37163248 None:intergenic 40.0%
!!! GCCTCTTTTTTTGAGAGGAA+AGG - chr4.2:37163224-37163243 None:intergenic 40.0%
AAAGGAGAAACCACTTGGAC+GGG - chr4.2:37163206-37163225 None:intergenic 45.0%
AAGAACCCGGATTACACTAC+AGG + chr4.2:37162114-37162133 MS.gene50068:CDS 45.0%
ACAGGTAAGAAGTGACACAC+TGG - chr4.2:37163758-37163777 None:intergenic 45.0%
ACCCACATAAGATGGTTCAC+AGG - chr4.2:37163776-37163795 None:intergenic 45.0%
AGAACCCAGATTACACTACC+GGG + chr4.2:37162598-37162617 MS.gene50068:CDS 45.0%
AGAACCCGGATTACACTACA+GGG + chr4.2:37162115-37162134 MS.gene50068:CDS 45.0%
AGAGGAAAGGAGAAACCACT+TGG - chr4.2:37163211-37163230 None:intergenic 45.0%
AGTCTTTGTCTCCCCTGTAA+TGG - chr4.2:37163366-37163385 None:intergenic 45.0%
CACACAGAAGCTTTCAAGGT+AGG + chr4.2:37163128-37163147 MS.gene50068:CDS 45.0%
CACTTGGACGGGAAATTGAA+GGG - chr4.2:37163195-37163214 None:intergenic 45.0%
CAGGAAAATGAGGAGAGTGA+AGG - chr4.2:37160276-37160295 None:intergenic 45.0%
CATCAATGCCAGAGACTTGA+AGG + chr4.2:37161900-37161919 MS.gene50068:CDS 45.0%
CCAAACAGCTGGATTCCAAT+GGG + chr4.2:37162541-37162560 MS.gene50068:CDS 45.0%
CGGATATAACACTCATACCC+AGG + chr4.2:37162953-37162972 MS.gene50068:CDS 45.0%
CTAAGCGATCCTTACATGAC+TGG + chr4.2:37162057-37162076 MS.gene50068:CDS 45.0%
GAAAGGAGAAACCACTTGGA+CGG - chr4.2:37163207-37163226 None:intergenic 45.0%
GAACCTTACAACACTGCCTA+TGG + chr4.2:37162789-37162808 MS.gene50068:CDS 45.0%
GATCCAAGTTAGGAGATTGG+GGG - chr4.2:37163109-37163128 None:intergenic 45.0%
GCATTGGAACCCACATAAGA+TGG - chr4.2:37163784-37163803 None:intergenic 45.0%
GCCAAACAACTGGATTCCAA+TGG + chr4.2:37162681-37162700 MS.gene50068:CDS 45.0%
GCCATTTGGACACATCTGAT+TGG + chr4.2:37163678-37163697 MS.gene50068:CDS 45.0%
GCTTCTGTGTGATCCAAGTT+AGG - chr4.2:37163119-37163138 None:intergenic 45.0%
GGAAGTTATGTAAGGTCCAC+TGG - chr4.2:37162505-37162524 None:intergenic 45.0%
GGAAGTTGTGTAAGGTCCAT+TGG - chr4.2:37162895-37162914 None:intergenic 45.0%
GGACCTTACACAACTTCCTA+TGG + chr4.2:37162897-37162916 MS.gene50068:CDS 45.0%
GGATCACACAGAAGCTTTCA+AGG + chr4.2:37163124-37163143 MS.gene50068:CDS 45.0%
GGATGACAAGCTACCCATTT+GGG - chr4.2:37163542-37163561 None:intergenic 45.0%
GGCAGTGTTGTAAGGTTCAT+TGG - chr4.2:37162787-37162806 None:intergenic 45.0%
GGTAGTGTAATCTGGGTTCT+TGG - chr4.2:37162598-37162617 None:intergenic 45.0%
GTAAGGTCCACTGGATTCAT+GGG - chr4.2:37162496-37162515 None:intergenic 45.0%
GTGTTCAAGGTCTCACTTGT+CGG + chr4.2:37163617-37163636 MS.gene50068:CDS 45.0%
GTTCATTGGATGCATGGGTA+TGG - chr4.2:37162773-37162792 None:intergenic 45.0%
TAGACATGAAGCCAAACAGC+TGG + chr4.2:37162530-37162549 MS.gene50068:CDS 45.0%
TCCACTGGATTCATGGGTAT+GGG - chr4.2:37162490-37162509 None:intergenic 45.0%
TGAACCCGGTAGTGTAATCT+GGG - chr4.2:37162605-37162624 None:intergenic 45.0%
TGGACATGAAGCCAAACAAC+TGG + chr4.2:37162671-37162690 MS.gene50068:CDS 45.0%
TGGATGACAAGCTACCCATT+TGG - chr4.2:37163543-37163562 None:intergenic 45.0%
TGTAAGGTCCACTGGATTCA+TGG - chr4.2:37162497-37162516 None:intergenic 45.0%
TGTACCCGGTAGTGTAATCT+GGG - chr4.2:37162746-37162765 None:intergenic 45.0%
TGTACCCTGTAGTGTAATCC+GGG - chr4.2:37162122-37162141 None:intergenic 45.0%
TGTAGTGTAATCCGGGTTCT+TGG - chr4.2:37162115-37162134 None:intergenic 45.0%
TTGAACCCGGTAGTGTAATC+TGG - chr4.2:37162606-37162625 None:intergenic 45.0%
TTGTACCCGGTAGTGTAATC+TGG - chr4.2:37162747-37162766 None:intergenic 45.0%
! ATATTTGGGGTCTTGGAGAG+GGG + chr4.2:37160079-37160098 MS.gene50068:intron 45.0%
! GATATTTGGGGTCTTGGAGA+GGG + chr4.2:37160078-37160097 MS.gene50068:intron 45.0%
! TACAGGGACTAGAGTGTTCA+AGG + chr4.2:37163604-37163623 MS.gene50068:CDS 45.0%
! TGATATTTGGGGTCTTGGAG+AGG + chr4.2:37160077-37160096 MS.gene50068:intron 45.0%
! TTTGGCTTCATGTCTACCGT+AGG - chr4.2:37162526-37162545 None:intergenic 45.0%
!! ATGCTTGTTGTGTTTGGTGG+TGG - chr4.2:37162019-37162038 None:intergenic 45.0%
!! GAGGCTTTTGAAGGTTGCAT+TGG - chr4.2:37163800-37163819 None:intergenic 45.0%
!! AAATAAAAATAAAAAAAATG+GGG - chr4.2:37160596-37160615 None:intergenic 5.0%
!!! CAATATTATTATTTATTTTT+TGG + chr4.2:37161708-37161727 MS.gene50068:intron 5.0%
!!! TTTTATTTTTTCTTTATATT+TGG + chr4.2:37161512-37161531 MS.gene50068:intron 5.0%
ACACACTGGAGAGTTCTTCC+CGG - chr4.2:37163744-37163763 None:intergenic 50.0%
ACCACCTCCAACGATCTAGA+AGG + chr4.2:37163083-37163102 MS.gene50068:CDS 50.0%
ACCCATACCCATGAATCCAG+TGG + chr4.2:37162486-37162505 MS.gene50068:CDS 50.0%
AGATCGTTGGAGGTGGTAGA+TGG - chr4.2:37163080-37163099 None:intergenic 50.0%
AGCGATCCTTACATGACTGG+CGG + chr4.2:37162060-37162079 MS.gene50068:CDS 50.0%
ATCGTTGGAGGTGGTAGATG+GGG - chr4.2:37163078-37163097 None:intergenic 50.0%
ATGGGTATGGGTCTTGAACC+CGG - chr4.2:37162619-37162638 None:intergenic 50.0%
CACCAACAAACCCTCACCAA+CGG + chr4.2:37163463-37163482 MS.gene50068:CDS 50.0%
CAGCTCTACCTTCAAGTCTC+TGG - chr4.2:37161911-37161930 None:intergenic 50.0%
CCACCGAAGGCAGTTATGTA+AGG - chr4.2:37162654-37162673 None:intergenic 50.0%
CCACTTGGACGGGAAATTGA+AGG - chr4.2:37163196-37163215 None:intergenic 50.0%
CCATACATTACCCAGTACGG+AGG + chr4.2:37161964-37161983 MS.gene50068:CDS 50.0%
CCCATTGGAATCCAGCTGTT+TGG - chr4.2:37162544-37162563 None:intergenic 50.0%
CCTCCGTACTGGGTAATGTA+TGG - chr4.2:37161967-37161986 None:intergenic 50.0%
CCTTACATAACTGCCTTCGG+TGG + chr4.2:37162651-37162670 MS.gene50068:CDS 50.0%
CCTTCAATTTCCCGTCCAAG+TGG + chr4.2:37163193-37163212 MS.gene50068:CDS 50.0%
CTAAGCGAGCCTTACATGAC+TGG + chr4.2:37162171-37162190 MS.gene50068:CDS 50.0%
CTGTAACCGCCAGTCATGTA+AGG - chr4.2:37162183-37162202 None:intergenic 50.0%
GAAGGTAGAGCTGCAAGATC+AGG + chr4.2:37161918-37161937 MS.gene50068:CDS 50.0%
GATCGTTGGAGGTGGTAGAT+GGG - chr4.2:37163079-37163098 None:intergenic 50.0%
GCCAAACAGCTGGATTCCAA+TGG + chr4.2:37162540-37162559 MS.gene50068:CDS 50.0%
GCTGCAAGATCAGGATCAAG+TGG + chr4.2:37161927-37161946 MS.gene50068:CDS 50.0%
GGCGGTTACAGACTTGCATA+TGG + chr4.2:37162192-37162211 MS.gene50068:CDS 50.0%
GGGAGTTGTGACATTGCAAG+AGG - chr4.2:37163257-37163276 None:intergenic 50.0%
GTCCACTGGATTCATGGGTA+TGG - chr4.2:37162491-37162510 None:intergenic 50.0%
TCACTTGTCGGTGATGCGAA+TGG + chr4.2:37163629-37163648 MS.gene50068:CDS 50.0%
TGGTTTACTGCCTCCGTACT+GGG - chr4.2:37161977-37161996 None:intergenic 50.0%
TTCTGTAAAGGGACAGCCGT+TGG - chr4.2:37163482-37163501 None:intergenic 50.0%
TTGGTTTACTGCCTCCGTAC+TGG - chr4.2:37161978-37161997 None:intergenic 50.0%
! ATGGGTATGGGTCTTGTACC+CGG - chr4.2:37162136-37162155 None:intergenic 50.0%
! GAGCATGGACTCTAGAGAGT+TGG - chr4.2:37163393-37163412 None:intergenic 50.0%
! TTTGGCTTCATGTCCACCGA+AGG - chr4.2:37162667-37162686 None:intergenic 50.0%
!! TGGTGGTGGTGTATGAAGCT+GGG - chr4.2:37162005-37162024 None:intergenic 50.0%
!! TTGGTGGTGGTGTATGAAGC+TGG - chr4.2:37162006-37162025 None:intergenic 50.0%
AGCGAGCCTTACATGACTGG+CGG + chr4.2:37162174-37162193 MS.gene50068:CDS 55.0%
AGGCAGCTTGGCATTAAGGC+CGG + chr4.2:37163722-37163741 MS.gene50068:CDS 55.0%
AGGCCCCCAATCTCCTAACT+TGG + chr4.2:37163103-37163122 MS.gene50068:CDS 55.0%
CTGTACCCGCCAGTCATGTA+AGG - chr4.2:37162069-37162088 None:intergenic 55.0%
GCCTTCTAGATCGTTGGAGG+TGG - chr4.2:37163087-37163106 None:intergenic 55.0%
GCGATCCTTACATGACTGGC+GGG + chr4.2:37162061-37162080 MS.gene50068:CDS 55.0%
GGCGGGTACAGACTTGCATA+TGG + chr4.2:37162078-37162097 MS.gene50068:CDS 55.0%
TAAAGGGACAGCCGTTGGTG+AGG - chr4.2:37163477-37163496 None:intergenic 55.0%
TCGTTGGAGGTGGTAGATGG+GGG - chr4.2:37163077-37163096 None:intergenic 55.0%
TTGGGGGCCTTCTAGATCGT+TGG - chr4.2:37163093-37163112 None:intergenic 55.0%
! AAAGGGACAGCCGTTGGTGA+GGG - chr4.2:37163476-37163495 None:intergenic 55.0%
! AGCCGTTGGTGAGGGTTTGT+TGG - chr4.2:37163468-37163487 None:intergenic 55.0%
! TGGTAGATGGGGGGCCTTTT+GGG - chr4.2:37163067-37163086 None:intergenic 55.0%
!! CGTTGGTGAGGGTTTGTTGG+TGG - chr4.2:37163465-37163484 None:intergenic 55.0%
CGTTGGAGGTGGTAGATGGG+GGG - chr4.2:37163076-37163095 None:intergenic 60.0%
GCGAGCCTTACATGACTGGC+GGG + chr4.2:37162175-37162194 MS.gene50068:CDS 60.0%
GGCAGCTTGGCATTAAGGCC+GGG + chr4.2:37163723-37163742 MS.gene50068:CDS 60.0%
GGGGCCTTCTAGATCGTTGG+AGG - chr4.2:37163090-37163109 None:intergenic 60.0%
! GTGGTAGATGGGGGGCCTTT+TGG - chr4.2:37163068-37163087 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 37160004 37163826 37160004 ID=MS.gene50068
chr4.2 mRNA 37160004 37163826 37160004 ID=MS.gene50068.t1;Parent=MS.gene50068
chr4.2 exon 37160004 37160057 37160004 ID=MS.gene50068.t1.exon1;Parent=MS.gene50068.t1
chr4.2 CDS 37160004 37160057 37160004 ID=cds.MS.gene50068.t1;Parent=MS.gene50068.t1
chr4.2 exon 37161889 37163826 37161889 ID=MS.gene50068.t1.exon2;Parent=MS.gene50068.t1
chr4.2 CDS 37161889 37163826 37161889 ID=cds.MS.gene50068.t1;Parent=MS.gene50068.t1
Gene Sequence

>MS.gene50068

ATGGCTACTGCACTAGCACTTCAGTTCCTCACAGTAGTTCTTTTCTTCTTTATGGTAATTCAATTGCACATAGTGATATTTGGGGTCTTGGAGAGGGGAGATGAGAGATTATTTTAAATTTTAGTTATTTGATTAAGTTATTAGATACGGAGAAAAAAGAGGGAAGCAAAATTTCTTCAAAAATAATTTCACATTTAAACATAAATGACATTTTACTAATTGTCATAATAAATATATTTCAATCGTGAAAATTGTAATTTTATGCATTTCCTTCACTCTCCTCATTTTCCTGTAATGAACCAAACAATGCAATTTCTATAGGTGTCCTTGAAAATAAAAATTTACATTTAGCATCTATAACATGTATAGTTTCAAGTTATAATTATAAATTTTTTAAGGAGTTATAATTATAACTTAGTTTTGTGATAAAACAAAAATGTTGTTTATTCATCGATTTTTAAATGCATTTTTATATCTTCAATCCTATAATTTTTCTTTCAATATAATCTTTTAGCCACATTTAATTGTGTGACTCAATGAAAACACTAGAGTTTTGTTGAATATTGTGATTATGGTGTAGCCTTGGTCACCCCATTTTTTTTATTTTTATTTATCAGTTAGTTAGTATATTTTTTTATAGCCATCTATATCTTGTAGATTTAGCTACTTTTTTTTTTTTAATGAACAATAAAAATGTTTTGTTGAAAACACTTGCACTTTTTCTAATGAATAAAATCTGATCTTTGTTGTAGATGTATTCAATACCGACACTTTAGTTTTATTTACATTTGAATAGAAGGCAAGCGGGCAACAAACTGCATATAACAGTGTACATGTGTGTTGCGTAGCCTTAGCCATCCCATTTATTGTTTTACTTATCATCTAGCTAGTATATTTGTTTTAGTTATCTAGTAATCACGTATATTTAGCTACTTTGAACAAGTCTTATTAGCTAAAGATGAGTATAAATAGAAAAGTAAAAAAATTACAGCTTGATTGATAAATTTAGTCGTCATGTTTGGACACTTCTAGTTTTATAGTAATTATGCATAAAGCTTTGTTAACAAGGAAAATTGTCAACACAAGATTTAGAAGCAAAATATATGATGAGTTATCTACAAATAACTTAAATGATTCTACATAATGTTATGATTTGTAGTTTTTGATTCTAACATTGATTTTAACATATAAATTTATAGCTCAACTCACTTTTCAAGAATATATTTGAATACAATTCAATTTACCTTCAAGTCACTTTTAATCAGAATAAATTTTAAAGAATCAATTCACTTAAAAATAAGTTCTTATCACTGCATAACCAAATATACAATTAGTTTGTTTAGTACAAATTAATTATGGACGAATTTAATTTCTTGAAACATCATCGAACTCGACCTTTCTAAATAGTATTTTCGGTTTTTATTTCATTTGCACTACTTTAATTTACGTTTCTATAGAACTATTTTTTCCTTATTAGTTAAGTTTAATATATTAACTTTATAGACATGTTTTATTTTTTCTTTATATTTGGCCCTCGTAAAATTTCCTCTAACTCTGCCATTCTATATAATCATTTATTGCTATATTTTTTTAAAATTTAATTATTTCATAGTTATTTCTTTTTTTTTTGACGAATATTTCATTGTTATTTTAAATCTCGTATTAATTTTGCTGACATCAATATTCTACAAAACTCTATGTCGTCAATATTATTATTTATTTTTTGGTTAAAGGTCATTGCAGTTAAAACGTGAAGCAATAGTAATATCAACTTGGCTAATCACTTGGACATTAATATTCTTTTTTTTTTTTTGACGGGATTAATATGTTTTTTTTGTTTTTTGAAAAAGTCTTTCAATTTATATCCTCGTTGTTGTCATTGTAGATTGGTCAAGACATCAATGCCAGAGACTTGAAGGTAGAGCTGCAAGATCAGGATCAAGTGGACTCGAATGATAAACCATACATTACCCAGTACGGAGGCAGTAAACCAAATTACCCAGCTTCATACACCACCACCAAACACAACAAGCATATATCACATCTCGAAAAGCTAAGCGATCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATACCCATGAATCTAATGAAAAGCTAAGCGAGCCTTACATGACTGGCGGTTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATACCCATGAATCTAATGAAAAGCTAAGCGAGCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAGACCCATACCCATGAATCTAATGAAAAGCTAAGCGAGCCTTACATGACTGGCGGGTACAGACTTGCATATGGTAAACATGAAGCCAAGAACCCGGATTACACTACAGGGTACAAAACCCATACCCATGAATCCAGTGGACCTTACATAACTTCCTACGGTAGACATGAAGCCAAACAGCTGGATTCCAATGGGCCTTACATTACTCAATATGGTAAACATGAAACCAAGAACCCAGATTACACTACCGGGTTCAAGACCCATACCCATGAATCCATTGAACCTTACATAACTGCCTTCGGTGGACATGAAGCCAAACAACTGGATTCCAATGGACCTTATATAAATCAATATGGTAAACATGAAACCAAGAACCCAGATTACACTACCGGGTACAAGACCCATACCCATGCATCCAATGAACCTTACAACACTGCCTATGGTAAACATGAAGCCAAACAATCAGAATCCAATGGGCCTTACATAACTCAATATGGTAAACATGAAGCCAAACAACAAGAATCCAATGGACCTTACACAACTTCCTATGGTAAACAAGAAGCAAAGCAACAAAGTTACAATACCGGATATAACACTCATACCCAGGAATCTAATGAATTGTACGTATCTGCATATGGTAACCATGAAGCCAAGCAACCAAATTACCTTAACGGGTACATATCTGTTGGAAATGACCCAAAAGGCCCCCCATCTACCACCTCCAACGATCTAGAAGGCCCCCAATCTCCTAACTTGGATCACACAGAAGCTTTCAAGGTAGGATTTTTCAATTTGGATGATCTTTATGTTGGAAATGCAATGACCCTTCAATTTCCCGTCCAAGTGGTTTCTCCTTTCCTCTCAAAAAAAGAGGCTGACTCCATTCCTCTTGCAATGTCACAACTCCCAAGTGTTCTTCAACTCTTCTCAATCCCTGAAGATTCTCCTCAAGCTAAATCCATGATAAATTCACTTGGAGAGTGTGAATCGGAAACCATTACAGGGGAGACAAAGACTTGTGCCAACTCTCTAGAGTCCATGCTCGAATTTGTTGACATGATCATTGGTTCAGACGCCAAACATAGTATTCTCACCACCAACAAACCCTCACCAACGGCTGTCCCTTTACAGAAGTACACCATTTTGGAAGTATCACATGATATTGATGCTCCCAAATGGGTAGCTTGTCATCCACTCTCATATCCATATGCCATTTACTTTTGTCATTACATAGCTACAGGGACTAGAGTGTTCAAGGTCTCACTTGTCGGTGATGCGAATGGAGATAAAATGGAAGCTGTTGGAATTTGCCATTTGGACACATCTGATTGGAACCCAGATCATATTATATTTAGGCAGCTTGGCATTAAGGCCGGGAAGAACTCTCCAGTGTGTCACTTCTTACCTGTGAACCATCTTATGTGGGTTCCAATGCAACCTTCAAAAGCCTCCATGTGA
Protein sequence

>MS.gene50068.t1

MATALALQFLTVVLFFFMIGQDINARDLKVELQDQDQVDSNDKPYITQYGGSKPNYPASYTTTKHNKHISHLEKLSDPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESNEKLSEPYMTGGYRLAYGKHEAKNPDYTTGYKTHTHESSGPYITSYGRHEAKQLDSNGPYITQYGKHETKNPDYTTGFKTHTHESIEPYITAFGGHEAKQLDSNGPYINQYGKHETKNPDYTTGYKTHTHASNEPYNTAYGKHEAKQSESNGPYITQYGKHEAKQQESNGPYTTSYGKQEAKQQSYNTGYNTHTQESNELYVSAYGNHEAKQPNYLNGYISVGNDPKGPPSTTSNDLEGPQSPNLDHTEAFKVGFFNLDDLYVGNAMTLQFPVQVVSPFLSKKEADSIPLAMSQLPSVLQLFSIPEDSPQAKSMINSLGECESETITGETKTCANSLESMLEFVDMIIGSDAKHSILTTNKPSPTAVPLQKYTILEVSHDIDAPKWVACHPLSYPYAIYFCHYIATGTRVFKVSLVGDANGDKMEAVGICHLDTSDWNPDHIIFRQLGIKAGKNSPVCHFLPVNHLMWVPMQPSKASM