Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50177.t1 | XP_003616397.1 | 99 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 2.10E-223 | 784.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50177.t1 | Q9Y312 | 28.8 | 400 | 245 | 9 | 1 | 386 | 6 | 379 | 5.6e-36 | 153.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50177.t1 | G7KBQ2 | 99.0 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 1.5e-223 | 784.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene50177 | 0.810487 | 1.12E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene50177 | 0.801604 | 8.32E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene50177 | 0.803488 | 3.40E-49 | -1.69E-46 |
| MS.gene052611 | MS.gene50177 | 0.809857 | 1.54E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene50177 | 0.808964 | 2.39E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene50177 | 0.840099 | 1.07E-57 | -1.69E-46 |
| MS.gene054994 | MS.gene50177 | 0.80549 | 1.30E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene50177 | 0.818718 | 1.69E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene50177 | 0.803381 | 3.58E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene50177 | 0.80012 | 1.67E-48 | -1.69E-46 |
| MS.gene060803 | MS.gene50177 | 0.805603 | 1.23E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68979 | MS.gene50177 | PPI |
| MS.gene50177 | MS.gene044498 | PPI |
| MS.gene50177 | MS.gene033017 | PPI |
| MS.gene50177 | MS.gene038800 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50177.t1 | MTR_5g079770 | 98.977 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 0.0 | 800 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50177.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene50177.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene50177.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene50177.t1 | AT1G66510 | 66.006 | 353 | 108 | 5 | 45 | 390 | 1 | 348 | 8.42e-174 | 489 |
Find 84 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGGTAATTGTTCGTAAA+TGG | 0.186934 | 5.4:+62670544 | MS.gene50177:intron |
| ACTTTACTGGCAAAAGATTA+TGG | 0.257738 | 5.4:+62672618 | MS.gene50177:CDS |
| CACTATTGTTGGATGATTCT+TGG | 0.258501 | 5.4:+62674198 | MS.gene50177:CDS |
| AAGCTATTAGAGAGCAATCT+AGG | 0.318940 | 5.4:+62674863 | MS.gene50177:CDS |
| GTTTGCATTTATCGCCCTTA+TGG | 0.327929 | 5.4:+62672674 | MS.gene50177:CDS |
| CAGCAGAACAACGCTGTTGA+TGG | 0.346353 | 5.4:+62674896 | MS.gene50177:CDS |
| TCCTTGGTTAGCCTTCTGTT+TGG | 0.383792 | 5.4:+62672810 | MS.gene50177:CDS |
| ATTGGATTCTTTATTGATGC+TGG | 0.406561 | 5.4:+62669201 | MS.gene50177:CDS |
| ATTGTTTACCTGAGTATCAA+TGG | 0.419994 | 5.4:-62666787 | None:intergenic |
| ATGGAATTTGACCGACAACT+TGG | 0.429503 | 5.4:+62671341 | MS.gene50177:CDS |
| ACAATTGCTAGAGACTTTAC+TGG | 0.432701 | 5.4:+62672605 | MS.gene50177:CDS |
| ATCTTAGCCACTATGAAGAT+TGG | 0.447384 | 5.4:+62671372 | MS.gene50177:CDS |
| TAGTAGACAAAATGAGTTCC+CGG | 0.450062 | 5.4:-62668437 | None:intergenic |
| TGTTCGTAAATGGGACCAAC+AGG | 0.453079 | 5.4:+62670554 | MS.gene50177:CDS |
| GTTGGCAAGTTGCAGAGAAA+AGG | 0.455685 | 5.4:+62671638 | MS.gene50177:CDS |
| GCAGGTAATTGTTCGTAAAT+GGG | 0.457276 | 5.4:+62670545 | MS.gene50177:intron |
| ATCTATTATCAACTAAAATA+CGG | 0.457832 | 5.4:+62674143 | MS.gene50177:CDS |
| CTAGATGGGTCTGTCATTGA+TGG | 0.466944 | 5.4:+62674650 | MS.gene50177:CDS |
| GGTTCTGAAGACTTGCTCCT+TGG | 0.470331 | 5.4:+62672642 | MS.gene50177:CDS |
| TCATAGTGGCTAAGATTGTA+AGG | 0.472984 | 5.4:-62671365 | None:intergenic |
| TGACTAGCCGTTCCTCCTGT+TGG | 0.475000 | 5.4:-62670569 | None:intergenic |
| AACACTGACTTCTCCTCCAA+TGG | 0.477819 | 5.4:-62671522 | None:intergenic |
| AGAAAATATACTTACTGCTT+CGG | 0.477936 | 5.4:-62672838 | None:intergenic |
| GATAATTCTGCAACATCTGT+TGG | 0.478381 | 5.4:+62671620 | MS.gene50177:CDS |
| CTAGGATGAAAGATATAGTC+TGG | 0.482454 | 5.4:+62671307 | MS.gene50177:intron |
| GGGTATTAAGATGATTCCTC+CGG | 0.487283 | 5.4:+62668418 | MS.gene50177:CDS |
| ATTTCTCCAGTGGAAATCCT+TGG | 0.497323 | 5.4:+62672794 | MS.gene50177:CDS |
| GTGTCTAGTGCTTTCTCCAT+TGG | 0.503391 | 5.4:-62671584 | None:intergenic |
| GGCTTCCTGCAGAGATGGAA+AGG | 0.504025 | 5.4:+62669164 | MS.gene50177:intron |
| GATTGTAAGGCCCAAGTTGT+CGG | 0.506606 | 5.4:-62671352 | None:intergenic |
| TATTGATGCTGGCCCTTCAG+AGG | 0.511306 | 5.4:+62669212 | MS.gene50177:CDS |
| TAAATAATACCTTACAATGA+TGG | 0.519108 | 5.4:-62674244 | None:intergenic |
| TTGGCAAGTTGCAGAGAAAA+GGG | 0.521046 | 5.4:+62671639 | MS.gene50177:CDS |
| TATGGATTCTCAAACAGCGT+TGG | 0.521890 | 5.4:+62666704 | None:intergenic |
| GATCAAATCAAACCTGGTTG+AGG | 0.524670 | 5.4:-62668465 | None:intergenic |
| AGTCAAAGTATCTGAGGAAG+AGG | 0.525053 | 5.4:+62670587 | MS.gene50177:CDS |
| TCATCTAACATCTCTACTAC+AGG | 0.528298 | 5.4:-62675040 | None:intergenic |
| TAACACAGGACCACTATTGT+TGG | 0.533398 | 5.4:+62674187 | MS.gene50177:CDS |
| CCTGAGTATCAATGGCAACA+AGG | 0.534352 | 5.4:-62666779 | None:intergenic |
| TGGAATTTGACCGACAACTT+GGG | 0.538353 | 5.4:+62671342 | MS.gene50177:CDS |
| ACACTGACTTCTCCTCCAAT+GGG | 0.539146 | 5.4:-62671521 | None:intergenic |
| CAAGAATCATCCAACAATAG+TGG | 0.539982 | 5.4:-62674197 | None:intergenic |
| CCTTGTTGCCATTGATACTC+AGG | 0.546257 | 5.4:+62666779 | MS.gene50177:CDS |
| GGCTAACCAAGGATTTCCAC+TGG | 0.549600 | 5.4:-62672800 | None:intergenic |
| ATCTTAATACCCTTAAAAGA+AGG | 0.553188 | 5.4:-62668407 | None:intergenic |
| TATTTATGAGCAGAGCCCAT+TGG | 0.553357 | 5.4:+62671506 | MS.gene50177:intron |
| CCACGTGTTGTAAAATGCAA+GGG | 0.556834 | 5.4:+62671680 | MS.gene50177:CDS |
| TTATGAGCAGAGCCCATTGG+AGG | 0.557137 | 5.4:+62671509 | MS.gene50177:intron |
| ATGCAAGGGGATCAGTGGAC+AGG | 0.557605 | 5.4:+62671694 | MS.gene50177:CDS |
| AGCTATTAGAGAGCAATCTA+GGG | 0.559485 | 5.4:+62674864 | MS.gene50177:CDS |
| TCCACGTGTTGTAAAATGCA+AGG | 0.560327 | 5.4:+62671679 | MS.gene50177:CDS |
| AAGAGCATCATCGAACGGCT+TGG | 0.567694 | 5.4:+62671419 | MS.gene50177:CDS |
| ATCGTTTCCAATCTTCATAG+TGG | 0.569850 | 5.4:-62671379 | None:intergenic |
| CGTTAGAAGCATTTCTCCAG+TGG | 0.577996 | 5.4:+62672784 | MS.gene50177:CDS |
| TCAATAAAGAATCCAATCAT+CGG | 0.579744 | 5.4:-62669195 | None:intergenic |
| TCTACTATAGTTCCTCAACC+AGG | 0.581273 | 5.4:+62668453 | MS.gene50177:CDS |
| CTGAGTATCAATGGCAACAA+GGG | 0.594931 | 5.4:-62666778 | None:intergenic |
| CTTCGGTGCAGCCAAACAGA+AGG | 0.598687 | 5.4:-62672821 | None:intergenic |
| AAACGCTCCTAAAACACCAA+TGG | 0.600774 | 5.4:+62671568 | MS.gene50177:CDS |
| TATTAGAGAGCAATCTAGGG+TGG | 0.601228 | 5.4:+62674867 | MS.gene50177:CDS |
| AGCACTAGACACGCAGCTGA+AGG | 0.603110 | 5.4:+62671595 | MS.gene50177:CDS |
| AATGGGACCAACAGGAGGAA+CGG | 0.609015 | 5.4:+62670562 | MS.gene50177:CDS |
| GGTATTAAGATGATTCCTCC+GGG | 0.612119 | 5.4:+62668419 | MS.gene50177:CDS |
| TGGCAACAAGGGTGTACTGA+GGG | 0.612425 | 5.4:-62666767 | None:intergenic |
| GTAAAATGCAAGGGGATCAG+TGG | 0.615109 | 5.4:+62671689 | MS.gene50177:CDS |
| ATTAGAGAGCAATCTAGGGT+GGG | 0.617313 | 5.4:+62674868 | MS.gene50177:CDS |
| ATGCAAACTGCAGTTCTCCA+AGG | 0.619224 | 5.4:-62672659 | None:intergenic |
| AGCCGTGTTCGTGTGTGGAA+AGG | 0.621192 | 5.4:-62673570 | None:intergenic |
| TTACAAAGAGCATCATCGAA+CGG | 0.632571 | 5.4:+62671414 | MS.gene50177:CDS |
| AGCCTTTCCACACACGAACA+CGG | 0.635124 | 5.4:+62673568 | MS.gene50177:intron |
| TGAATAGCCGTGTTCGTGTG+TGG | 0.635232 | 5.4:-62673575 | None:intergenic |
| CGGACTACAGAAAGATCGCA+AGG | 0.640325 | 5.4:+62674163 | MS.gene50177:CDS |
| ACGGCTAGTCAAAGTATCTG+AGG | 0.646658 | 5.4:+62670581 | MS.gene50177:CDS |
| CACGTGTTGTAAAATGCAAG+GGG | 0.646735 | 5.4:+62671681 | MS.gene50177:CDS |
| TACTTCTTTGAATCTTGACA+AGG | 0.647721 | 5.4:+62671721 | MS.gene50177:CDS |
| GCCAAACAGAAGGCTAACCA+AGG | 0.675388 | 5.4:-62672811 | None:intergenic |
| GTACTTTGATGAGAACGATG+AGG | 0.679398 | 5.4:+62674922 | MS.gene50177:CDS |
| TCGTAAATGGGACCAACAGG+AGG | 0.680154 | 5.4:+62670557 | MS.gene50177:CDS |
| ATGGCAACAAGGGTGTACTG+AGG | 0.692263 | 5.4:-62666768 | None:intergenic |
| AAAGATCGCAAGGATAACAC+AGG | 0.694703 | 5.4:+62674173 | MS.gene50177:CDS |
| TTACTGGCAAAAGATTATGG+AGG | 0.700059 | 5.4:+62672621 | MS.gene50177:CDS |
| GGGTGTACTGAGGGACATCG+AGG | 0.709510 | 5.4:-62666758 | None:intergenic |
| ACGAACACGGCTATTCACCA+AGG | 0.734563 | 5.4:+62673581 | MS.gene50177:CDS |
| TAGACAAAATGAGTTCCCGG+AGG | 0.766673 | 5.4:-62668434 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 62666705 | 62675081 | 62666705 | ID=MS.gene50177 |
| chr5.4 | mRNA | 62666705 | 62675081 | 62666705 | ID=MS.gene50177.t1;Parent=MS.gene50177 |
| chr5.4 | exon | 62666705 | 62666800 | 62666705 | ID=MS.gene50177.t1.exon1;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62666705 | 62666800 | 62666705 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62668392 | 62668474 | 62668392 | ID=MS.gene50177.t1.exon2;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62668392 | 62668474 | 62668392 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62669176 | 62669233 | 62669176 | ID=MS.gene50177.t1.exon3;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62669176 | 62669233 | 62669176 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62670549 | 62670608 | 62670549 | ID=MS.gene50177.t1.exon4;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62670549 | 62670608 | 62670549 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62671311 | 62671440 | 62671311 | ID=MS.gene50177.t1.exon5;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62671311 | 62671440 | 62671311 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62671519 | 62671742 | 62671519 | ID=MS.gene50177.t1.exon6;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62671519 | 62671742 | 62671519 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62672603 | 62672695 | 62672603 | ID=MS.gene50177.t1.exon7;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62672603 | 62672695 | 62672603 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62672774 | 62672845 | 62672774 | ID=MS.gene50177.t1.exon8;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62672774 | 62672845 | 62672774 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62673570 | 62673602 | 62673570 | ID=MS.gene50177.t1.exon9;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62673570 | 62673602 | 62673570 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62674131 | 62674256 | 62674131 | ID=MS.gene50177.t1.exon10;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62674131 | 62674256 | 62674131 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62674632 | 62674688 | 62674632 | ID=MS.gene50177.t1.exon11;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62674632 | 62674688 | 62674632 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62674848 | 62674943 | 62674848 | ID=MS.gene50177.t1.exon12;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62674848 | 62674943 | 62674848 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| chr5.4 | exon | 62675034 | 62675081 | 62675034 | ID=MS.gene50177.t1.exon13;Parent=MS.gene50177.t1 |
| chr5.4 | CDS | 62675034 | 62675081 | 62675034 | ID=cds.MS.gene50177.t1;Parent=MS.gene50177.t1 |
| Gene Sequence |
| Protein sequence |
