AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51453

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51453.t1 MTR_1g073860 97.635 296 6 1 29 323 27 322 0.0 573
MS.gene51453.t1 MTR_1g044155 66.990 309 80 7 34 323 54 359 1.22e-106 315
MS.gene51453.t1 MTR_1g079810 54.633 313 110 10 35 323 1 305 1.09e-79 245
MS.gene51453.t1 MTR_3g068035 59.910 222 71 5 103 312 86 301 1.18e-67 214
MS.gene51453.t1 MTR_5g091630 56.364 220 76 5 96 315 47 246 1.89e-64 204
MS.gene51453.t1 MTR_5g011520 74.026 154 40 0 96 249 100 253 5.55e-64 205
MS.gene51453.t1 MTR_8g036060 60.262 229 84 6 96 323 86 308 3.69e-63 202
MS.gene51453.t1 MTR_7g080980 50.923 271 108 5 46 304 1 258 9.37e-63 200
MS.gene51453.t1 MTR_3g100470 50.556 180 85 2 88 263 66 245 4.25e-58 189
MS.gene51453.t1 MTR_4g098450 57.789 199 73 3 103 299 83 272 2.91e-57 187
MS.gene51453.t1 MTR_5g080580 67.133 143 47 0 103 245 96 238 3.64e-52 175
MS.gene51453.t1 MTR_1g073870 50.820 244 54 6 83 323 39 219 5.23e-48 160
MS.gene51453.t1 MTR_5g066020 42.063 252 117 7 61 300 9 243 7.95e-44 151
MS.gene51453.t1 MTR_5g089600 32.143 168 103 3 87 243 68 235 5.32e-28 109
MS.gene51453.t1 MTR_5g010330 33.500 200 113 7 95 282 103 294 1.06e-22 96.7
MS.gene51453.t1 MTR_1g053685 29.775 178 115 2 95 262 84 261 7.24e-22 93.6
MS.gene51453.t1 MTR_4g057525 32.370 173 105 3 81 243 23 193 1.93e-21 91.7
MS.gene51453.t1 MTR_4g057487 32.075 159 98 2 95 243 16 174 3.78e-21 90.9
MS.gene51453.t1 MTR_5g089700 33.548 155 93 3 94 239 73 226 6.31e-21 90.1
MS.gene51453.t1 MTR_6g061670 39.655 116 67 1 123 235 130 245 1.03e-20 91.3
MS.gene51453.t1 MTR_6g061670 39.655 116 67 1 123 235 125 240 1.26e-20 90.9
MS.gene51453.t1 MTR_1g079780 37.500 136 81 2 105 236 88 223 2.82e-20 90.1
MS.gene51453.t1 MTR_5g089090 36.242 149 87 2 96 237 28 175 3.29e-20 87.4
MS.gene51453.t1 MTR_1g032910 30.864 162 100 2 94 243 99 260 1.44e-19 87.4
MS.gene51453.t1 MTR_1g061530 39.474 114 65 2 126 235 144 257 2.47e-19 87.8
MS.gene51453.t1 MTR_4g098460 36.986 146 79 4 96 235 57 195 7.28e-18 82.4
MS.gene51453.t1 MTR_7g116320 39.823 113 68 0 123 235 143 255 2.61e-17 82.0
MS.gene51453.t1 MTR_7g077120 34.731 167 100 3 94 254 125 288 3.47e-17 81.6
MS.gene51453.t1 MTR_4g069110 39.175 97 59 0 94 190 32 128 1.38e-16 76.6
MS.gene51453.t1 MTR_8g036160 35.294 153 91 2 126 274 145 293 2.18e-16 79.3
MS.gene51453.t1 MTR_4g098460 36.000 150 80 4 96 235 57 200 3.41e-16 77.8
MS.gene51453.t1 MTR_8g031100 40.566 106 60 1 89 191 118 223 3.85e-16 78.6
MS.gene51453.t1 MTR_8g098390 31.944 144 94 3 114 254 157 299 4.02e-16 78.6
MS.gene51453.t1 MTR_7g116320 38.400 125 73 1 123 243 143 267 9.32e-16 76.3
MS.gene51453.t1 MTR_1g076710 37.931 116 68 2 123 235 134 248 1.07e-15 77.0
MS.gene51453.t1 MTR_7g074935 47.761 67 35 0 126 192 130 196 1.26e-15 77.0
MS.gene51453.t1 MTR_4g055813 36.364 143 82 4 113 247 4 145 1.01e-14 73.6
MS.gene51453.t1 MTR_8g078930 31.210 157 96 4 127 272 5 160 2.63e-14 70.1
MS.gene51453.t1 MTR_4g055803 40.517 116 63 3 126 235 128 243 5.56e-14 72.8
MS.gene51453.t1 MTR_5g011620 28.873 142 97 2 127 266 119 258 1.16e-13 72.0
MS.gene51453.t1 MTR_4g055833 36.719 128 75 3 122 243 121 248 6.21e-13 68.9
MS.gene51453.t1 MTR_4g101990 43.284 67 38 0 126 192 147 213 8.47e-13 68.6
MS.gene51453.t1 MTR_6g060670 38.739 111 66 2 126 236 180 288 2.45e-12 67.0
MS.gene51453.t1 MTR_5g011610 28.169 142 98 3 127 266 62 201 2.78e-12 67.0
MS.gene51453.t1 MTR_5g011680 29.286 140 91 3 126 258 145 283 4.18e-12 66.2
MS.gene51453.t1 MTR_1g071850 30.769 143 71 3 103 245 37 151 3.77e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51453.t1 AT4G14465 61.486 296 92 7 35 323 1 281 4.70e-103 303
MS.gene51453.t1 AT3G04570 62.762 239 59 5 35 245 1 237 3.02e-79 244
MS.gene51453.t1 AT1G76500 63.102 187 55 4 72 247 49 232 4.44e-63 202
MS.gene51453.t1 AT2G35270 65.161 155 54 0 96 250 78 232 1.66e-61 197
MS.gene51453.t1 AT2G45430 59.641 223 80 5 96 311 89 308 2.62e-61 198
MS.gene51453.t1 AT2G42940 64.286 154 55 0 96 249 53 206 1.58e-60 194
MS.gene51453.t1 AT4G22810 54.357 241 76 5 96 323 105 324 4.25e-60 195
MS.gene51453.t1 AT3G60870 55.330 197 77 3 53 240 7 201 1.50e-59 192
MS.gene51453.t1 AT4G12050 70.323 155 45 1 96 249 118 272 6.87e-57 187
MS.gene51453.t1 AT5G49700 43.725 247 109 7 88 323 49 276 7.75e-57 185
MS.gene51453.t1 AT3G55560 44.478 335 111 12 35 322 1 307 2.12e-54 180
MS.gene51453.t1 AT4G17800 55.340 206 66 2 103 302 89 274 3.18e-54 179
MS.gene51453.t1 AT4G35390 68.000 150 46 1 96 243 63 212 2.66e-50 169
MS.gene51453.t1 AT1G20900 59.259 189 60 2 74 245 56 244 8.94e-48 163
MS.gene51453.t1 AT1G14490 49.444 180 79 4 97 266 25 202 1.07e-44 152
MS.gene51453.t1 AT1G14490 49.444 180 79 4 97 266 6 183 1.24e-44 152
MS.gene51453.t1 AT4G17950 42.069 145 77 3 96 235 196 338 1.47e-22 97.8
MS.gene51453.t1 AT1G63470 36.986 146 85 3 96 235 147 291 3.25e-22 96.3
MS.gene51453.t1 AT5G62260 37.097 124 75 1 123 243 160 283 3.72e-21 92.0
MS.gene51453.t1 AT2G45850 39.597 149 80 4 95 235 131 277 2.32e-20 90.5
MS.gene51453.t1 AT2G45850 39.597 149 80 4 95 235 131 277 2.32e-20 90.5
MS.gene51453.t1 AT2G33620 37.241 145 87 3 94 235 135 278 3.65e-20 90.1
MS.gene51453.t1 AT2G33620 37.241 145 87 3 94 235 135 278 3.65e-20 90.1
MS.gene51453.t1 AT2G33620 37.241 145 87 3 94 235 135 278 3.65e-20 90.1
MS.gene51453.t1 AT2G33620 37.241 145 87 3 94 235 135 278 3.65e-20 90.1
MS.gene51453.t1 AT4G12080 39.316 117 68 1 126 239 173 289 3.99e-20 90.1
MS.gene51453.t1 AT5G62260 37.931 116 69 1 123 235 160 275 5.74e-20 90.1
MS.gene51453.t1 AT5G62260 37.931 116 69 1 123 235 160 275 5.74e-20 90.1
MS.gene51453.t1 AT5G51590 38.938 113 66 1 126 235 181 293 1.24e-19 89.4
MS.gene51453.t1 AT4G25320 35.220 159 83 3 97 235 123 281 1.30e-19 89.0
MS.gene51453.t1 AT3G04590 37.273 110 69 0 126 235 174 283 4.01e-19 87.8
MS.gene51453.t1 AT2G45850 38.667 150 82 4 95 236 131 278 5.71e-19 85.5
MS.gene51453.t1 AT3G04590 37.273 110 69 0 126 235 174 283 8.07e-19 85.9
MS.gene51453.t1 AT1G63480 30.811 185 107 6 96 260 124 307 6.95e-17 80.9
MS.gene51453.t1 AT1G63480 30.811 185 107 6 96 260 124 307 6.95e-17 80.9
MS.gene51453.t1 AT1G63480 30.811 185 107 6 96 260 124 307 6.95e-17 80.9
MS.gene51453.t1 AT1G63480 30.811 185 107 6 96 260 124 307 6.95e-17 80.9
MS.gene51453.t1 AT1G63480 30.811 185 107 6 96 260 124 307 6.95e-17 80.9
MS.gene51453.t1 AT1G63480 30.978 184 106 6 96 259 130 312 8.08e-17 80.5
MS.gene51453.t1 AT1G63480 30.978 184 106 6 96 259 130 312 8.08e-17 80.5
MS.gene51453.t1 AT5G46640 42.759 145 76 3 96 236 155 296 2.36e-16 79.3
MS.gene51453.t1 AT3G61310 34.694 147 90 2 95 235 133 279 1.11e-14 74.3
MS.gene51453.t1 AT4G00200 38.655 119 70 1 126 241 126 244 2.97e-14 72.8
MS.gene51453.t1 AT4G00200 38.655 119 70 1 126 241 126 244 2.97e-14 72.8
MS.gene51453.t1 AT4G22770 38.333 120 71 1 126 242 153 272 5.28e-14 72.0
MS.gene51453.t1 AT4G22770 38.333 120 71 1 126 242 153 272 5.28e-14 72.0

Find 112 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTTGGTGAGTGGGTTCC+TGG 0.152889 1.4:+60474236 None:intergenic
CCAAATTTGTTGCCTAATAA+TGG 0.209471 1.4:-60473498 MS.gene51453:CDS
CCATTATTAGGCAACAAATT+TGG 0.219989 1.4:+60473498 None:intergenic
ACAATTCAAGGAGGGTTATC+TGG 0.264024 1.4:-60474266 MS.gene51453:CDS
ACATGGCTCTTCAAAGCATT+TGG 0.272297 1.4:+60474029 None:intergenic
GTTACAAAGATGGGTGGTTT+TGG 0.295281 1.4:+60474062 None:intergenic
CTTGCCTGGGCTGCTGCTCA+TGG 0.299376 1.4:-60473453 MS.gene51453:CDS
CCCATCTGCCCTCCATTATT+AGG 0.325156 1.4:+60473486 None:intergenic
AAATACTACAACAGCAGTTC+TGG 0.340855 1.4:-60474303 MS.gene51453:CDS
CCACTCAACTCAACTCAATA+AGG 0.342276 1.4:+60473423 None:intergenic
AGTGTACTTGGCTGGAGGTC+AGG 0.342488 1.4:-60473778 MS.gene51453:CDS
GGTGCTGTTGTAGCACTTCA+TGG 0.358357 1.4:-60473873 MS.gene51453:CDS
CCTGCTCCACCAGGTTCAAC+TGG 0.375523 1.4:-60473807 MS.gene51453:CDS
TGAGCAAGGGACTCAGAAAC+TGG 0.380264 1.4:-60475415 MS.gene51453:CDS
GATCCAGGTGGTCTTCCTCT+TGG 0.385010 1.4:+60474095 None:intergenic
CATGGCTCTTCAAAGCATTT+GGG 0.396581 1.4:+60474030 None:intergenic
TGGGACATGAGGCTCTTGCC+TGG 0.399930 1.4:-60473467 MS.gene51453:CDS
GTAGTTGGAGGTTGTGTTGT+TGG 0.411543 1.4:-60473750 MS.gene51453:CDS
GGAGGTCAGGGTCAGGTAGT+TGG 0.413964 1.4:-60473765 MS.gene51453:CDS
GTGGTCTTCCTCTTGGTCTT+CGG 0.414167 1.4:+60474102 None:intergenic
TGCAACTGATGACCAATTCC+TGG 0.414250 1.4:+60473567 None:intergenic
GGATCGCCTCCACCAGGAAT+TGG 0.416217 1.4:-60473579 MS.gene51453:CDS
CAATTCAAGGAGGGTTATCT+GGG 0.426102 1.4:-60474265 MS.gene51453:CDS
CTACCTGGTCCTGCTCCACC+AGG 0.428797 1.4:-60473816 MS.gene51453:CDS
GCTATCTCTTGTTACAAAGA+TGG 0.435101 1.4:+60474052 None:intergenic
GTGCAAGGTGGAGGAGCGTC+TGG 0.440396 1.4:-60473603 MS.gene51453:CDS
CTTGGCTGGAGGTCAGGGTC+AGG 0.442843 1.4:-60473772 MS.gene51453:CDS
AACTGGTCTTACAGTGTACT+TGG 0.444380 1.4:-60473790 MS.gene51453:CDS
AGGAATTGGTCATCAGTTGC+AGG 0.445128 1.4:-60473565 MS.gene51453:CDS
GTTGGAACTCTTGTTGCAGT+TGG 0.451198 1.4:-60473732 MS.gene51453:CDS
CACTTGGTGAGTGGGTTCCT+GGG 0.452332 1.4:+60474237 None:intergenic
TTGGTCATCAGTTGCAGGGT+GGG 0.456584 1.4:-60473560 MS.gene51453:CDS
AATGAAGGGCCTGGTTCTGC+AGG 0.457424 1.4:-60473630 MS.gene51453:CDS
GCCTAATAATGGAGGGCAGA+TGG 0.462555 1.4:-60473487 MS.gene51453:CDS
AATCCTTGGTGGACAATTCA+AGG 0.465438 1.4:-60474278 MS.gene51453:CDS
TGCACACCTCCTGCAGAACC+AGG 0.466109 1.4:+60473621 None:intergenic
CTATCTCTTGTTACAAAGAT+GGG 0.474278 1.4:+60474053 None:intergenic
AAAGGCATGGAAGAAGGATC+TGG 0.482278 1.4:+60473534 None:intergenic
CCAGTTGAACCTGGTGGAGC+AGG 0.482936 1.4:+60473807 None:intergenic
CTGTTGTAGCACTTCATGGT+AGG 0.488288 1.4:-60473869 MS.gene51453:CDS
GTGTACTTGGCTGGAGGTCA+GGG 0.493209 1.4:-60473777 MS.gene51453:CDS
GGGACATGAGGCTCTTGCCT+GGG 0.498915 1.4:-60473466 MS.gene51453:CDS
CCCTTGCTCAGATGTATGAC+AGG 0.500235 1.4:+60475428 None:intergenic
TTCTGCTATATCAGCTCCAC+CGG 0.501001 1.4:+60473995 None:intergenic
CCTAATAATGGAGGGCAGAT+GGG 0.501432 1.4:-60473486 MS.gene51453:CDS
GTTTAAACCACTTGGTGAGT+GGG 0.503662 1.4:+60474229 None:intergenic
ACTTCAACTGCACCTTCTCT+AGG 0.505701 1.4:+60474131 None:intergenic
AGGTAGAAAAGTCCCGGTTA+AGG 0.506619 1.4:+60473833 None:intergenic
GATGATAATGATAATAATGA+AGG 0.507615 1.4:-60473645 MS.gene51453:CDS
TCTTCCATGAGCAGCAGCCC+AGG 0.509624 1.4:+60473449 None:intergenic
GGTTCTGCAGGAGGTGTGCA+AGG 0.509942 1.4:-60473618 MS.gene51453:CDS
GCAGGAGGTGTGCAAGGTGG+AGG 0.514025 1.4:-60473612 MS.gene51453:CDS
GGTCAGGGTCAGGTAGTTGG+AGG 0.515248 1.4:-60473762 MS.gene51453:CDS
CAGCAGTTCTGGCTAATCCT+TGG 0.516441 1.4:-60474292 MS.gene51453:CDS
CTTAGACAACCTTCAGCTCC+TGG 0.520193 1.4:-60473894 MS.gene51453:CDS
TTATTAGGCAACAAATTTGG+TGG 0.521583 1.4:+60473501 None:intergenic
TTATACAAAGGCATGGAAGA+AGG 0.526106 1.4:+60473528 None:intergenic
GGTCTTACAGTGTACTTGGC+TGG 0.537231 1.4:-60473786 MS.gene51453:CDS
AATGATAATAATGAAGGGCC+TGG 0.537636 1.4:-60473639 MS.gene51453:CDS
TGTTTAAACCACTTGGTGAG+TGG 0.539187 1.4:+60474228 None:intergenic
CAGTTCTGGCTAATCCTTGG+TGG 0.541662 1.4:-60474289 MS.gene51453:CDS
AGAGAAGGTGCAGTTGAAGT+TGG 0.547259 1.4:-60474128 MS.gene51453:CDS
TGCTTTGAAGAGCCATGTCA+TGG 0.550949 1.4:-60474024 MS.gene51453:CDS
TGGTGTTTGTTTAAACCACT+TGG 0.552489 1.4:+60474221 None:intergenic
GTTTGTGTTCTCAGTGGAAG+TGG 0.555754 1.4:-60473936 MS.gene51453:CDS
GAACCTGGTGGAGCAGGACC+AGG 0.556098 1.4:+60473813 None:intergenic
AATTTGTTGCCTAATAATGG+AGG 0.564310 1.4:-60473495 MS.gene51453:CDS
GTGTGGCACAATTCGCAAGA+AGG 0.565025 1.4:-60473971 MS.gene51453:CDS
TCTGGTGGATCGCCTCCACC+AGG 0.566478 1.4:-60473585 MS.gene51453:CDS
TGATTGTTAGGTTTGTGTTG+TGG 0.569261 1.4:+60474201 None:intergenic
AGCCATGTCATGGAGGTAGC+CGG 0.569908 1.4:-60474014 MS.gene51453:CDS
GGGTTATCTGGGGTTGACCC+AGG 0.573460 1.4:-60474254 MS.gene51453:CDS
CGCGGTGTTTGTGTTCTCAG+TGG 0.579327 1.4:-60473942 MS.gene51453:CDS
TCATTATCATCGTCATCAAG+TGG 0.580583 1.4:+60473657 None:intergenic
TGATGACCAATTCCTGGTGG+AGG 0.582864 1.4:+60473573 None:intergenic
ACTGTAAGACCAGTTGAACC+TGG 0.584188 1.4:+60473798 None:intergenic
AGACCAAGAGGAAGACCACC+TGG 0.587335 1.4:-60474098 MS.gene51453:CDS
ACCTGTCATACATCTGAGCA+AGG 0.587720 1.4:-60475429 MS.gene51453:CDS
AGGACCAGGTAGAAAAGTCC+CGG 0.591266 1.4:+60473827 None:intergenic
ACTTCATTCATATACACAAG+TGG 0.591925 1.4:-60475382 MS.gene51453:CDS
TGGCAAATTATACAAAGGCA+TGG 0.593563 1.4:+60473521 None:intergenic
TGGAGGGCAGATGGGACATG+AGG 0.594853 1.4:-60473478 MS.gene51453:CDS
GAAGGGCCTGGTTCTGCAGG+AGG 0.596026 1.4:-60473627 MS.gene51453:CDS
TTTGGTGGCAAATTATACAA+AGG 0.596262 1.4:+60473516 None:intergenic
TCTGCAGGAGGTGTGCAAGG+TGG 0.601460 1.4:-60473615 MS.gene51453:CDS
ACAACAGCACCAGGAGCTGA+AGG 0.601528 1.4:+60473885 None:intergenic
CACCGGCTACCTCCATGACA+TGG 0.602203 1.4:+60474012 None:intergenic
TGAAGTGCTACAACAGCACC+AGG 0.605157 1.4:+60473876 None:intergenic
ATGATAATGATAATAATGAA+GGG 0.605731 1.4:-60473644 MS.gene51453:CDS
ATTTGTTGCCTAATAATGGA+GGG 0.617852 1.4:-60473494 MS.gene51453:CDS
TCTCTTGTTACAAAGATGGG+TGG 0.617894 1.4:+60474056 None:intergenic
CAAGGTGGAGGAGCGTCTGG+TGG 0.620899 1.4:-60473600 MS.gene51453:CDS
GGAATTGGTCATCAGTTGCA+GGG 0.626929 1.4:-60473564 MS.gene51453:CDS
CTTGGTGGACAATTCAAGGA+GGG 0.630959 1.4:-60474274 MS.gene51453:CDS
AGCTGATATAGCAGAAAGTG+TGG 0.642948 1.4:-60473988 MS.gene51453:CDS
CTTACAGTGTACTTGGCTGG+AGG 0.650202 1.4:-60473783 MS.gene51453:CDS
ATTGGTCATCAGTTGCAGGG+TGG 0.653304 1.4:-60473561 MS.gene51453:CDS
TTGCTCAGATGTATGACAGG+TGG 0.660606 1.4:+60475431 None:intergenic
AACAGAGATGAACCTAGAGA+AGG 0.663205 1.4:-60474143 MS.gene51453:CDS
GTTGGTAACCGAAGACCAAG+AGG 0.671648 1.4:-60474110 MS.gene51453:CDS
CTCAACTCAACTCAATAAGG+TGG 0.676109 1.4:+60473426 None:intergenic
CCAGGAACCCACTCACCAAG+TGG 0.676419 1.4:-60474236 MS.gene51453:CDS
AACTGATGACCAATTCCTGG+TGG 0.683488 1.4:+60473570 None:intergenic
CCTCCTTGAATTGTCCACCA+AGG 0.691826 1.4:+60474275 None:intergenic
CATGTCATGGAGGTAGCCGG+TGG 0.697813 1.4:-60474011 MS.gene51453:CDS
GTAAGACCAGTTGAACCTGG+TGG 0.698978 1.4:+60473801 None:intergenic
CCTGTCATACATCTGAGCAA+GGG 0.699527 1.4:-60475428 MS.gene51453:CDS
AGTAGCATTAGTAAACGTAG+CGG 0.707477 1.4:+60473692 None:intergenic
AATTCAAGGAGGGTTATCTG+GGG 0.712203 1.4:-60474264 MS.gene51453:CDS
CCTTGGTGGACAATTCAAGG+AGG 0.716947 1.4:-60474275 MS.gene51453:CDS
TTTGAAGAGCCATGTCATGG+AGG 0.719459 1.4:-60474021 MS.gene51453:CDS
TTCGCAAGAAGGCGTCAACG+CGG 0.746882 1.4:-60473960 MS.gene51453:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATAAACAAACAAATAAA+TGG - chr1.4:60474209-60474228 MS.gene51453:CDS 15.0%
!! GAATAAACAAACAAATAAAT+GGG - chr1.4:60474210-60474229 MS.gene51453:CDS 15.0%
!! TATTTGTTTGTTTATTCTAT+TGG + chr1.4:60474207-60474226 None:intergenic 15.0%
!!! ATGATAATGATAATAATGAA+GGG - chr1.4:60475227-60475246 MS.gene51453:intron 15.0%
!!! GATGATAATGATAATAATGA+AGG - chr1.4:60475226-60475245 MS.gene51453:intron 20.0%
!!! TGTTTTCTTACTTACTTTAT+TGG + chr1.4:60473910-60473929 None:intergenic 20.0%
! AAGTTATTATGGGACAAAAA+GGG - chr1.4:60473865-60473884 MS.gene51453:CDS 25.0%
! AGCTGAAAAAAGAAACAAAA+GGG + chr1.4:60474404-60474423 None:intergenic 25.0%
! GCAAAAATCTCAGTTATTAT+AGG + chr1.4:60473644-60473663 None:intergenic 25.0%
! GCAAATGACTAAGTTATTAT+GGG - chr1.4:60473855-60473874 MS.gene51453:CDS 25.0%
! TAAGTTATTATGGGACAAAA+AGG - chr1.4:60473864-60473883 MS.gene51453:CDS 25.0%
! TGCAAATGACTAAGTTATTA+TGG - chr1.4:60473854-60473873 MS.gene51453:CDS 25.0%
!! ACGAAATGAGTCAATTATTT+TGG - chr1.4:60473822-60473841 MS.gene51453:CDS 25.0%
!! TACTCAAAATGATGATGATA+GGG + chr1.4:60474334-60474353 None:intergenic 25.0%
!! TTACTCAAAATGATGATGAT+AGG + chr1.4:60474335-60474354 None:intergenic 25.0%
!!! TTAGTTGTTTTTTGTGATGT+AGG + chr1.4:60474467-60474486 None:intergenic 25.0%
AACTAACTAACTATTTGAGG+AGG + chr1.4:60474045-60474064 None:intergenic 30.0%
ACTTCATTCATATACACAAG+TGG - chr1.4:60473489-60473508 MS.gene51453:CDS 30.0%
AGAGAATTAGATGCAATTAC+TGG + chr1.4:60474286-60474305 None:intergenic 30.0%
AGTTATTATGGGACAAAAAG+GGG - chr1.4:60473866-60473885 MS.gene51453:CDS 30.0%
CAAAACTAATCAAGCTCAAT+TGG - chr1.4:60474494-60474513 MS.gene51453:intron 30.0%
CCATTATTAGGCAACAAATT+TGG + chr1.4:60475376-60475395 None:intergenic 30.0%
CGAAACTAACTAACTATTTG+AGG + chr1.4:60474048-60474067 None:intergenic 30.0%
CTATCTCTTGTTACAAAGAT+GGG + chr1.4:60474821-60474840 None:intergenic 30.0%
GAGCTGAAAAAAGAAACAAA+AGG + chr1.4:60474405-60474424 None:intergenic 30.0%
TATTTGAGGAGGACAAATTA+AGG + chr1.4:60474034-60474053 None:intergenic 30.0%
TTATTAGGCAACAAATTTGG+TGG + chr1.4:60475373-60475392 None:intergenic 30.0%
TTTGTGATGTAGGAGATATA+TGG + chr1.4:60474457-60474476 None:intergenic 30.0%
! AAATGATGATGATAGGGTAA+AGG + chr1.4:60474328-60474347 None:intergenic 30.0%
! ATTTGTTGCCTAATAATGGA+GGG - chr1.4:60475377-60475396 MS.gene51453:intron 30.0%
! TTTGGTGGCAAATTATACAA+AGG + chr1.4:60475358-60475377 None:intergenic 30.0%
!! AATTTGTTGCCTAATAATGG+AGG - chr1.4:60475376-60475395 MS.gene51453:intron 30.0%
!! CCAAATTTGTTGCCTAATAA+TGG - chr1.4:60475373-60475392 MS.gene51453:intron 30.0%
!! CGTGATTTTCATTGATTGTT+AGG + chr1.4:60474685-60474704 None:intergenic 30.0%
!! TTTGAGATTTTGTCCTTAAC+CGG - chr1.4:60475025-60475044 MS.gene51453:intron 30.0%
AAATACTACAACAGCAGTTC+TGG - chr1.4:60474568-60474587 MS.gene51453:intron 35.0%
AATGAGAGAAACTTTGCACT+AGG + chr1.4:60473532-60473551 None:intergenic 35.0%
AATGATAATAATGAAGGGCC+TGG - chr1.4:60475232-60475251 MS.gene51453:intron 35.0%
AGTAGCATTAGTAAACGTAG+CGG + chr1.4:60475182-60475201 None:intergenic 35.0%
CTGGATGAGAATCAAGTAAA+TGG + chr1.4:60474267-60474286 None:intergenic 35.0%
GCTATCTCTTGTTACAAAGA+TGG + chr1.4:60474822-60474841 None:intergenic 35.0%
TCATTATCATCGTCATCAAG+TGG + chr1.4:60475217-60475236 None:intergenic 35.0%
TGATTGTTAGGTTTGTGTTG+TGG + chr1.4:60474673-60474692 None:intergenic 35.0%
TGGCAAATTATACAAAGGCA+TGG + chr1.4:60475353-60475372 None:intergenic 35.0%
TGGTGTTTGTTTAAACCACT+TGG + chr1.4:60474653-60474672 None:intergenic 35.0%
! AGCTGTATTGTTGTTGACAT+GGG + chr1.4:60473965-60473984 None:intergenic 35.0%
! AGTTGAGTCTTTGAAAGGTT+TGG - chr1.4:60474162-60474181 MS.gene51453:CDS 35.0%
! TTATACAAAGGCATGGAAGA+AGG + chr1.4:60475346-60475365 None:intergenic 35.0%
! TTGAGATTTTGTCCTTAACC+GGG - chr1.4:60475026-60475045 MS.gene51453:intron 35.0%
!! ACTTGAGTTGAGTCTTTGAA+AGG - chr1.4:60474157-60474176 MS.gene51453:CDS 35.0%
AACAGAGATGAACCTAGAGA+AGG - chr1.4:60474728-60474747 MS.gene51453:intron 40.0%
AATTCAAGGAGGGTTATCTG+GGG - chr1.4:60474607-60474626 MS.gene51453:intron 40.0%
ACAATTCAAGGAGGGTTATC+TGG - chr1.4:60474605-60474624 MS.gene51453:intron 40.0%
AGCTGATATAGCAGAAAGTG+TGG - chr1.4:60474883-60474902 MS.gene51453:intron 40.0%
CAATTCAAGGAGGGTTATCT+GGG - chr1.4:60474606-60474625 MS.gene51453:intron 40.0%
GTGAGTGAGCATCTTATGTT+AGG - chr1.4:60474359-60474378 MS.gene51453:intron 40.0%
TCTCTTGTTACAAAGATGGG+TGG + chr1.4:60474818-60474837 None:intergenic 40.0%
TGATAGGGTAAAGGAAAGTG+AGG + chr1.4:60474319-60474338 None:intergenic 40.0%
! AATCCTTGGTGGACAATTCA+AGG - chr1.4:60474593-60474612 MS.gene51453:intron 40.0%
! ACATGGCTCTTCAAAGCATT+TGG + chr1.4:60474845-60474864 None:intergenic 40.0%
! CATGGCTCTTCAAAGCATTT+GGG + chr1.4:60474844-60474863 None:intergenic 40.0%
! GAGCTGTATTGTTGTTGACA+TGG + chr1.4:60473966-60473985 None:intergenic 40.0%
! GCTGTATTGTTGTTGACATG+GGG + chr1.4:60473964-60473983 None:intergenic 40.0%
! GTTACAAAGATGGGTGGTTT+TGG + chr1.4:60474812-60474831 None:intergenic 40.0%
! GTTTAAACCACTTGGTGAGT+GGG + chr1.4:60474645-60474664 None:intergenic 40.0%
! TGTTTAAACCACTTGGTGAG+TGG + chr1.4:60474646-60474665 None:intergenic 40.0%
!! AACTGGTCTTACAGTGTACT+TGG - chr1.4:60475081-60475100 MS.gene51453:intron 40.0%
!!! TTTGGTCTGTTTTTGGATCC+AGG + chr1.4:60474794-60474813 None:intergenic 40.0%
AACTGATGACCAATTCCTGG+TGG + chr1.4:60475304-60475323 None:intergenic 45.0%
ACCTGTCATACATCTGAGCA+AGG - chr1.4:60473442-60473461 MS.gene51453:CDS 45.0%
ACTGTAAGACCAGTTGAACC+TGG + chr1.4:60475076-60475095 None:intergenic 45.0%
ACTTCAACTGCACCTTCTCT+AGG + chr1.4:60474743-60474762 None:intergenic 45.0%
AGGAATTGGTCATCAGTTGC+AGG - chr1.4:60475306-60475325 MS.gene51453:intron 45.0%
AGGTAGAAAAGTCCCGGTTA+AGG + chr1.4:60475041-60475060 None:intergenic 45.0%
CCTGTCATACATCTGAGCAA+GGG - chr1.4:60473443-60473462 MS.gene51453:CDS 45.0%
CTGTTGTAGCACTTCATGGT+AGG - chr1.4:60475002-60475021 MS.gene51453:intron 45.0%
GGAATTGGTCATCAGTTGCA+GGG - chr1.4:60475307-60475326 MS.gene51453:intron 45.0%
GTAGTTGGAGGTTGTGTTGT+TGG - chr1.4:60475121-60475140 MS.gene51453:intron 45.0%
GTTGGAACTCTTGTTGCAGT+TGG - chr1.4:60475139-60475158 MS.gene51453:intron 45.0%
GTTTGTGTTCTCAGTGGAAG+TGG - chr1.4:60474935-60474954 MS.gene51453:intron 45.0%
TGCAACTGATGACCAATTCC+TGG + chr1.4:60475307-60475326 None:intergenic 45.0%
TTCTGCTATATCAGCTCCAC+CGG + chr1.4:60474879-60474898 None:intergenic 45.0%
TTGCTCAGATGTATGACAGG+TGG + chr1.4:60473443-60473462 None:intergenic 45.0%
TTTGAAGAGCCATGTCATGG+AGG - chr1.4:60474850-60474869 MS.gene51453:intron 45.0%
! AAAGGCATGGAAGAAGGATC+TGG + chr1.4:60475340-60475359 None:intergenic 45.0%
! AGAGAAGGTGCAGTTGAAGT+TGG - chr1.4:60474743-60474762 MS.gene51453:intron 45.0%
! CCTAATAATGGAGGGCAGAT+GGG - chr1.4:60475385-60475404 MS.gene51453:CDS 45.0%
! CTTGGTGGACAATTCAAGGA+GGG - chr1.4:60474597-60474616 MS.gene51453:intron 45.0%
! TTAACCGGGACTTTTCTACC+TGG - chr1.4:60475040-60475059 MS.gene51453:intron 45.0%
!! TGCTTTGAAGAGCCATGTCA+TGG - chr1.4:60474847-60474866 MS.gene51453:intron 45.0%
!!! GGGTGGTTTTGGTCTGTTTT+TGG + chr1.4:60474801-60474820 None:intergenic 45.0%
AGGACCAGGTAGAAAAGTCC+CGG + chr1.4:60475047-60475066 None:intergenic 50.0%
ATTGGTCATCAGTTGCAGGG+TGG - chr1.4:60475310-60475329 MS.gene51453:intron 50.0%
CCCATCTGCCCTCCATTATT+AGG + chr1.4:60475388-60475407 None:intergenic 50.0%
CCCTTGCTCAGATGTATGAC+AGG + chr1.4:60473446-60473465 None:intergenic 50.0%
CCTCCTTGAATTGTCCACCA+AGG + chr1.4:60474599-60474618 None:intergenic 50.0%
CTTACAGTGTACTTGGCTGG+AGG - chr1.4:60475088-60475107 MS.gene51453:intron 50.0%
CTTAGACAACCTTCAGCTCC+TGG - chr1.4:60474977-60474996 MS.gene51453:intron 50.0%
GGTCTTACAGTGTACTTGGC+TGG - chr1.4:60475085-60475104 MS.gene51453:intron 50.0%
GTAAGACCAGTTGAACCTGG+TGG + chr1.4:60475073-60475092 None:intergenic 50.0%
GTGTGGCACAATTCGCAAGA+AGG - chr1.4:60474900-60474919 MS.gene51453:intron 50.0%
TGAGCAAGGGACTCAGAAAC+TGG - chr1.4:60473456-60473475 MS.gene51453:CDS 50.0%
TGATGACCAATTCCTGGTGG+AGG + chr1.4:60475301-60475320 None:intergenic 50.0%
TTGGTCATCAGTTGCAGGGT+GGG - chr1.4:60475311-60475330 MS.gene51453:intron 50.0%
! CAGCAGTTCTGGCTAATCCT+TGG - chr1.4:60474579-60474598 MS.gene51453:intron 50.0%
! CAGTTCTGGCTAATCCTTGG+TGG - chr1.4:60474582-60474601 MS.gene51453:intron 50.0%
! CCTTGGTGGACAATTCAAGG+AGG - chr1.4:60474596-60474615 MS.gene51453:intron 50.0%
! GCCTAATAATGGAGGGCAGA+TGG - chr1.4:60475384-60475403 MS.gene51453:CDS 50.0%
! GTGGTCTTCCTCTTGGTCTT+CGG + chr1.4:60474772-60474791 None:intergenic 50.0%
! TGAAGTGCTACAACAGCACC+AGG + chr1.4:60474998-60475017 None:intergenic 50.0%
!! GGTGCTGTTGTAGCACTTCA+TGG - chr1.4:60474998-60475017 MS.gene51453:intron 50.0%
!! GTTGGTAACCGAAGACCAAG+AGG - chr1.4:60474761-60474780 MS.gene51453:intron 50.0%
!!! GGTCTGTTTTTGGATCCAGG+TGG + chr1.4:60474791-60474810 None:intergenic 50.0%
ACAACAGCACCAGGAGCTGA+AGG + chr1.4:60474989-60475008 None:intergenic 55.0%
AGACCAAGAGGAAGACCACC+TGG - chr1.4:60474773-60474792 MS.gene51453:intron 55.0%
AGCCATGTCATGGAGGTAGC+CGG - chr1.4:60474857-60474876 MS.gene51453:intron 55.0%
AGTGTACTTGGCTGGAGGTC+AGG - chr1.4:60475093-60475112 MS.gene51453:intron 55.0%
CGCGGTGTTTGTGTTCTCAG+TGG - chr1.4:60474929-60474948 MS.gene51453:intron 55.0%
GATCCAGGTGGTCTTCCTCT+TGG + chr1.4:60474779-60474798 None:intergenic 55.0%
GTGTACTTGGCTGGAGGTCA+GGG - chr1.4:60475094-60475113 MS.gene51453:intron 55.0%
! CACTTGGTGAGTGGGTTCCT+GGG + chr1.4:60474637-60474656 None:intergenic 55.0%
!! AATGAAGGGCCTGGTTCTGC+AGG - chr1.4:60475241-60475260 MS.gene51453:intron 55.0%
!! TTCGCAAGAAGGCGTCAACG+CGG - chr1.4:60474911-60474930 MS.gene51453:intron 55.0%
CATGTCATGGAGGTAGCCGG+TGG - chr1.4:60474860-60474879 MS.gene51453:intron 60.0%
CCAGGAACCCACTCACCAAG+TGG - chr1.4:60474635-60474654 MS.gene51453:intron 60.0%
CCAGTTGAACCTGGTGGAGC+AGG + chr1.4:60475067-60475086 None:intergenic 60.0%
CCTGCTCCACCAGGTTCAAC+TGG - chr1.4:60475064-60475083 MS.gene51453:intron 60.0%
GGAGGTCAGGGTCAGGTAGT+TGG - chr1.4:60475106-60475125 MS.gene51453:intron 60.0%
GGATCGCCTCCACCAGGAAT+TGG - chr1.4:60475292-60475311 MS.gene51453:intron 60.0%
GGGACATGAGGCTCTTGCCT+GGG - chr1.4:60475405-60475424 MS.gene51453:CDS 60.0%
GGTCAGGGTCAGGTAGTTGG+AGG - chr1.4:60475109-60475128 MS.gene51453:intron 60.0%
TCTTCCATGAGCAGCAGCCC+AGG + chr1.4:60475425-60475444 None:intergenic 60.0%
TGCACACCTCCTGCAGAACC+AGG + chr1.4:60475253-60475272 None:intergenic 60.0%
TGGAGGGCAGATGGGACATG+AGG - chr1.4:60475393-60475412 MS.gene51453:CDS 60.0%
TGGGACATGAGGCTCTTGCC+TGG - chr1.4:60475404-60475423 MS.gene51453:CDS 60.0%
! CACCGGCTACCTCCATGACA+TGG + chr1.4:60474862-60474881 None:intergenic 60.0%
! CCACTTGGTGAGTGGGTTCC+TGG + chr1.4:60474638-60474657 None:intergenic 60.0%
! GGGTTATCTGGGGTTGACCC+AGG - chr1.4:60474617-60474636 MS.gene51453:intron 60.0%
! GGTTCTGCAGGAGGTGTGCA+AGG - chr1.4:60475253-60475272 MS.gene51453:intron 60.0%
! TCTGCAGGAGGTGTGCAAGG+TGG - chr1.4:60475256-60475275 MS.gene51453:intron 60.0%
CTACCTGGTCCTGCTCCACC+AGG - chr1.4:60475055-60475074 MS.gene51453:intron 65.0%
CTTGCCTGGGCTGCTGCTCA+TGG - chr1.4:60475418-60475437 MS.gene51453:CDS 65.0%
CTTGGCTGGAGGTCAGGGTC+AGG - chr1.4:60475099-60475118 MS.gene51453:intron 65.0%
GAACCTGGTGGAGCAGGACC+AGG + chr1.4:60475061-60475080 None:intergenic 65.0%
! GCAGGAGGTGTGCAAGGTGG+AGG - chr1.4:60475259-60475278 MS.gene51453:intron 65.0%
! TCTGGTGGATCGCCTCCACC+AGG - chr1.4:60475286-60475305 MS.gene51453:intron 65.0%
!! CAAGGTGGAGGAGCGTCTGG+TGG - chr1.4:60475271-60475290 MS.gene51453:intron 65.0%
!! GAAGGGCCTGGTTCTGCAGG+AGG - chr1.4:60475244-60475263 MS.gene51453:intron 65.0%
!! GTGCAAGGTGGAGGAGCGTC+TGG - chr1.4:60475268-60475287 MS.gene51453:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 60473437 60475456 60473437 ID=MS.gene51453
chr1.4 mRNA 60473437 60475456 60473437 ID=MS.gene51453.t1;Parent=MS.gene51453
chr1.4 exon 60475379 60475456 60475379 ID=MS.gene51453.t1.exon1;Parent=MS.gene51453.t1
chr1.4 CDS 60475379 60475456 60475379 ID=cds.MS.gene51453.t1;Parent=MS.gene51453.t1
chr1.4 exon 60473437 60474330 60473437 ID=MS.gene51453.t1.exon2;Parent=MS.gene51453.t1
chr1.4 CDS 60473437 60474330 60473437 ID=cds.MS.gene51453.t1;Parent=MS.gene51453.t1
Gene Sequence

>MS.gene51453

ATGCCACCTGTCATACATCTGAGCAAGGGACTCAGAAACTGGAACAATCATAACTTCATTCATATACACAAGTGGTTCGTTAGCTTTATCTGCCTAGTGCAAAGTTTCTCTCATTTTATTAAATTGAAATCTTGTTTATTTGAAATGTTGTTTATTTCATTTTTTTATCAAGTAGTATAATAACTTAAATAATACTCACTTCATCCTATAATAACTGAGATTTTTGCTCGTTTTACACGTATTAAGAAAAATGTATAACTGAAAAAGAGAAATATAGTTTTGAGTGTTTTGTCAAAATATATTGAATTAGAAGTGATGAAAGTATCATTAATATATTTTTTTAAAGTAGTTATAATTAAAAAAAATATTAATATAGCATTGAAAAACGAAATGAGTCAATTATTTTGGTGTTTTTTTTGCAAATGACTAAGTTATTATGGGACAAAAAGGGGTATCAATAAACGACAAAACCAATAAAGTAAGTAAGAAAACAAATAAGTAAGAAAAAAAAATATATTCTATCTCCCCATGTCAACAACAATACAGCTCACAACAACAATGATGAATTCAATTTATTCAAAAAATTTCTTCTCTCCTTAATTTGTCCTCCTCAAATAGTTAGTTAGTTTCGTGTTCTTCTCTAATTACTTCATTATTATAGCACTTTTGTCTCATAGTCTATGATCTAATTTTTTATTTCTTAATTCTCTCTCTCTTTGTACTTGAGTTGAGTCTTTGAAAGGTTTGGTATATCTTCTATCTTGTAACCAATAGAATAAACAAACAAATAAATGGGAAAAAAATTAGACAGTGTTTTTTTAATCAAACCATTTACTTGATTCTCATCCAGTAATTGCATCTAATTCTCTTTTCTAAGCACCTCACTTTCCTTTACCCTATCATCATCATTTTGAGTAAGTTAGTGAGTGAGCATCTTATGTTAGGTCATCATAGACATACATATCCCTTTTGTTTCTTTTTTCAGCTCTCTTATTGAGTTTGACTTTTTTGTTTCATCCATATATCTCCTACATCACAAAAAACAACTAAAATCAAACAAAACTAATCAAGCTCAATTGGTTTCTCATATTCTTATAAACAAAAGCATCATAATCTTCTTCTTAAGTGTAGAAATACTACAACAGCAGTTCTGGCTAATCCTTGGTGGACAATTCAAGGAGGGTTATCTGGGGTTGACCCAGGAACCCACTCACCAAGTGGTTTAAACAAACACCACAACACAAACCTAACAATCAATGAAAATCACGATGAAGAAGAAGACGTTGACGACAACAGAGATGAACCTAGAGAAGGTGCAGTTGAAGTTGGTAACCGAAGACCAAGAGGAAGACCACCTGGATCCAAAAACAGACCAAAACCACCCATCTTTGTAACAAGAGATAGCCCAAATGCTTTGAAGAGCCATGTCATGGAGGTAGCCGGTGGAGCTGATATAGCAGAAAGTGTGGCACAATTCGCAAGAAGGCGTCAACGCGGTGTTTGTGTTCTCAGTGGAAGTGGCTCTGTAGCTAATGTTACTCTTAGACAACCTTCAGCTCCTGGTGCTGTTGTAGCACTTCATGGTAGGTTTGAGATTTTGTCCTTAACCGGGACTTTTCTACCTGGTCCTGCTCCACCAGGTTCAACTGGTCTTACAGTGTACTTGGCTGGAGGTCAGGGTCAGGTAGTTGGAGGTTGTGTTGTTGGAACTCTTGTTGCAGTTGGATCTGTTATGCTTATTGCCGCTACGTTTACTAATGCTACTTATGAGAGACTTCCACTTGATGACGATGATAATGATAATAATGAAGGGCCTGGTTCTGCAGGAGGTGTGCAAGGTGGAGGAGCGTCTGGTGGATCGCCTCCACCAGGAATTGGTCATCAGTTGCAGGGTGGGCTTCCAGATCCTTCTTCCATGCCTTTGTATAATTTGCCACCAAATTTGTTGCCTAATAATGGAGGGCAGATGGGACATGAGGCTCTTGCCTGGGCTGCTGCTCATGGAAGACCACCTTATTGA
Protein sequence

>MS.gene51453.t1

MPPVIHLSKGLRNWNNHNFIHIHKWFCRNTTTAVLANPWWTIQGGLSGVDPGTHSPSGLNKHHNTNLTINENHDEEEDVDDNRDEPREGAVEVGNRRPRGRPPGSKNRPKPPIFVTRDSPNALKSHVMEVAGGADIAESVAQFARRRQRGVCVLSGSGSVANVTLRQPSAPGAVVALHGRFEILSLTGTFLPGPAPPGSTGLTVYLAGGQGQVVGGCVVGTLVAVGSVMLIAATFTNATYERLPLDDDDNDNNEGPGSAGGVQGGGASGGSPPPGIGHQLQGGLPDPSSMPLYNLPPNLLPNNGGQMGHEALAWAAAHGRPPY