Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53119.t1 | XP_024639118.1 | 92.6 | 282 | 21 | 0 | 1 | 282 | 1 | 282 | 1.50E-151 | 545.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53119.t1 | Q9FMS6 | 56.2 | 274 | 114 | 3 | 10 | 280 | 7 | 277 | 1.7e-82 | 307.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53119.t1 | A0A396HQ65 | 92.6 | 282 | 21 | 0 | 1 | 282 | 1 | 282 | 1.1e-151 | 545.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53119 | MS.gene025628 | PPI |
| MS.gene034250 | MS.gene53119 | PPI |
| MS.gene034816 | MS.gene53119 | PPI |
| MS.gene72977 | MS.gene53119 | PPI |
| MS.gene065709 | MS.gene53119 | PPI |
| MS.gene72978 | MS.gene53119 | PPI |
| MS.gene034815 | MS.gene53119 | PPI |
| MS.gene007154 | MS.gene53119 | PPI |
| MS.gene025629 | MS.gene53119 | PPI |
| MS.gene41100 | MS.gene53119 | PPI |
| MS.gene53119 | MS.gene41097 | PPI |
| MS.gene53119 | MS.gene21541 | PPI |
| MS.gene065707 | MS.gene53119 | PPI |
| MS.gene41101 | MS.gene53119 | PPI |
| MS.gene000609 | MS.gene53119 | PPI |
| MS.gene065708 | MS.gene53119 | PPI |
| MS.gene053225 | MS.gene53119 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53119.t1 | AT5G22250 | 56.204 | 274 | 114 | 3 | 10 | 280 | 7 | 277 | 9.30e-105 | 306 |
| MS.gene53119.t1 | AT3G44260 | 55.390 | 269 | 111 | 5 | 15 | 280 | 17 | 279 | 3.17e-98 | 290 |
| MS.gene53119.t1 | AT5G10960 | 46.182 | 275 | 139 | 6 | 7 | 280 | 1 | 267 | 4.89e-80 | 243 |
| MS.gene53119.t1 | AT2G32070 | 48.315 | 267 | 129 | 6 | 15 | 280 | 10 | 268 | 1.93e-79 | 242 |
| MS.gene53119.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 3.65e-75 | 231 |
| MS.gene53119.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 3.65e-75 | 231 |
| MS.gene53119.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 5.09e-75 | 231 |
| MS.gene53119.t1 | AT1G15920 | 45.324 | 278 | 142 | 6 | 9 | 282 | 8 | 279 | 9.20e-72 | 223 |
| MS.gene53119.t1 | AT1G15920 | 45.324 | 278 | 142 | 6 | 9 | 282 | 8 | 279 | 9.20e-72 | 223 |
| MS.gene53119.t1 | AT1G61470 | 39.024 | 246 | 125 | 8 | 19 | 259 | 4 | 229 | 4.14e-42 | 146 |
| MS.gene53119.t1 | AT1G06450 | 34.545 | 275 | 157 | 10 | 10 | 281 | 3 | 257 | 2.84e-39 | 140 |
| MS.gene53119.t1 | AT1G27820 | 36.260 | 262 | 140 | 9 | 13 | 267 | 2 | 243 | 3.47e-36 | 131 |
| MS.gene53119.t1 | AT1G27890 | 37.255 | 255 | 135 | 9 | 19 | 267 | 3 | 238 | 1.18e-34 | 127 |
| MS.gene53119.t1 | AT3G44240 | 35.622 | 233 | 129 | 8 | 31 | 260 | 3 | 217 | 5.06e-33 | 121 |
Find 58 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATGGCGCTTATGATTT+TGG | 0.117389 | 5.4:-32231600 | MS.gene53119:CDS |
| AAATACGCTGGAGTAGTATT+TGG | 0.224745 | 5.4:-32231303 | MS.gene53119:CDS |
| TGAATATGGTGAATATTCTT+TGG | 0.278766 | 5.4:-32232000 | MS.gene53119:CDS |
| CGTGATGTTCTTCCTGAATA+TGG | 0.278966 | 5.4:-32232014 | MS.gene53119:CDS |
| CAAACTTCTCTGACAATGAT+TGG | 0.307379 | 5.4:+32232071 | None:intergenic |
| CTTGGACTCACTTTAATAGA+TGG | 0.314324 | 5.4:-32231852 | MS.gene53119:CDS |
| CAATCATTGTCAGAGAAGTT+TGG | 0.315172 | 5.4:-32232070 | MS.gene53119:CDS |
| AAGAAATCTGCCTGATAGTT+TGG | 0.321613 | 5.4:-32231553 | MS.gene53119:CDS |
| AACATCTTGGAAATGTTATT+CGG | 0.335246 | 5.4:-32231519 | MS.gene53119:CDS |
| TCGATAGACACCGAGTTTCC+TGG | 0.362514 | 5.4:-32231975 | MS.gene53119:CDS |
| AATCATTGTCAGAGAAGTTT+GGG | 0.392755 | 5.4:-32232069 | MS.gene53119:CDS |
| CGATTATCGGTGTCAAAATC+AGG | 0.397384 | 5.4:+32231816 | None:intergenic |
| AGTTTAAGATCATCAACATT+TGG | 0.398324 | 5.4:+32231882 | None:intergenic |
| ACACCGATAATCGCTACATT+TGG | 0.399135 | 5.4:-32231806 | MS.gene53119:CDS |
| CTCAATGTGGATCGGGTTGT+TGG | 0.408633 | 5.4:-32231417 | MS.gene53119:CDS |
| TTGCCAAATGTAGCGATTAT+CGG | 0.410093 | 5.4:+32231803 | None:intergenic |
| AAAGAATATTCACCATATTC+AGG | 0.416250 | 5.4:+32232002 | None:intergenic |
| AGGTGGCGATGGTCGACTTT+TGG | 0.420934 | 5.4:+32231939 | None:intergenic |
| CATTGTATGGTGGTCTTGAT+CGG | 0.438293 | 5.4:-32231452 | MS.gene53119:CDS |
| AACTATCAGGCAGATTTCTT+CGG | 0.444161 | 5.4:+32231556 | None:intergenic |
| TGTCAACAAGCTGTCGGATC+CGG | 0.457649 | 5.4:+32231380 | None:intergenic |
| TATTCAAGAAATCAATGACT+TGG | 0.469568 | 5.4:-32231629 | MS.gene53119:CDS |
| CGCTACTGCAATGCATTGTA+TGG | 0.477422 | 5.4:-32231465 | MS.gene53119:CDS |
| ATGGCAGACGTTTCATAAGT+TGG | 0.479969 | 5.4:-32231358 | MS.gene53119:CDS |
| TGTAGTAATCAGAGGGTTGA+AGG | 0.480186 | 5.4:+32231919 | None:intergenic |
| GCTGAACTAATGTTGACGTC+TGG | 0.481099 | 5.4:-32231657 | MS.gene53119:CDS |
| ATTGTATGGTGGTCTTGATC+GGG | 0.514058 | 5.4:-32231451 | MS.gene53119:CDS |
| TTGTCGACGAAGCAAATCAA+TGG | 0.516920 | 5.4:+32231731 | None:intergenic |
| AAGTACACTCAATGTGGATC+GGG | 0.524067 | 5.4:-32231424 | MS.gene53119:CDS |
| TTCAATCGTTGTGTCTCTCA+AGG | 0.540052 | 5.4:-32231699 | MS.gene53119:CDS |
| GTTGGAAGGTCTCATCAATC+CGG | 0.540408 | 5.4:-32231399 | MS.gene53119:CDS |
| ATGTGGATCGGGTTGTTGGA+AGG | 0.540595 | 5.4:-32231413 | MS.gene53119:CDS |
| ATTCAAGAAATCAATGACTT+GGG | 0.542864 | 5.4:-32231628 | MS.gene53119:CDS |
| GATCTTAAACTCATCCAACT+TGG | 0.545194 | 5.4:-32231870 | MS.gene53119:CDS |
| TCTTGGAAATGTTATTCGGA+AGG | 0.546839 | 5.4:-32231515 | MS.gene53119:CDS |
| CAAGTACACTCAATGTGGAT+CGG | 0.548190 | 5.4:-32231425 | MS.gene53119:CDS |
| CAATGGAATCCTTGTTGTGA+GGG | 0.555858 | 5.4:+32231748 | None:intergenic |
| CTATTAAAGTGAGTCCAAGT+TGG | 0.558411 | 5.4:+32231856 | None:intergenic |
| TTTGGTGTATGGATAACTCC+AGG | 0.559457 | 5.4:+32231957 | None:intergenic |
| CAGAGGGTTGAAGGTGGCGA+TGG | 0.559485 | 5.4:+32231928 | None:intergenic |
| ATTGATTTGCTTCGTCGACA+AGG | 0.559543 | 5.4:-32231729 | MS.gene53119:CDS |
| TTCGGATCAATATCTTCACC+AGG | 0.564436 | 5.4:+32231574 | None:intergenic |
| GAAGAACATCACGAATGAGA+TGG | 0.565749 | 5.4:+32232024 | None:intergenic |
| TCAACATTAGTTCAGCAAAA+CGG | 0.571611 | 5.4:+32231664 | None:intergenic |
| GATCCGACAGCTTGTTGACA+TGG | 0.577163 | 5.4:-32231377 | MS.gene53119:CDS |
| CATACAATGCATTGCAGTAG+CGG | 0.583860 | 5.4:+32231466 | None:intergenic |
| TTGATTTGCTTCGTCGACAA+GGG | 0.585778 | 5.4:-32231728 | MS.gene53119:CDS |
| GACGTATCTGTAGTAATCAG+AGG | 0.593946 | 5.4:+32231911 | None:intergenic |
| TACTGCAATGCATTGTATGG+TGG | 0.597106 | 5.4:-32231462 | MS.gene53119:CDS |
| ATGGATAACTCCAGGAAACT+CGG | 0.598400 | 5.4:+32231965 | None:intergenic |
| ACGTATCTGTAGTAATCAGA+GGG | 0.599199 | 5.4:+32231912 | None:intergenic |
| CTCACTTTAATAGATGGTTG+CGG | 0.607202 | 5.4:-32231846 | MS.gene53119:CDS |
| CTGCCATGTCAACAAGCTGT+CGG | 0.613501 | 5.4:+32231374 | None:intergenic |
| TCAATGGAATCCTTGTTGTG+AGG | 0.615657 | 5.4:+32231747 | None:intergenic |
| GGTTGCAAGTACACTCAATG+TGG | 0.665808 | 5.4:-32231430 | MS.gene53119:CDS |
| TGAGCGTGACCCTCACAACA+AGG | 0.672608 | 5.4:-32231757 | MS.gene53119:CDS |
| TATCGGTGTCAAAATCAGGA+AGG | 0.683593 | 5.4:+32231820 | None:intergenic |
| AGTAATCAGAGGGTTGAAGG+TGG | 0.690515 | 5.4:+32231922 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAATATTCACCATATTC+AGG | + | chr5.4:32231395-32231414 | None:intergenic | 25.0% |
| ! | AACATCTTGGAAATGTTATT+CGG | - | chr5.4:32231875-32231894 | MS.gene53119:CDS | 25.0% |
| ! | ATTCAAGAAATCAATGACTT+GGG | - | chr5.4:32231766-32231785 | MS.gene53119:CDS | 25.0% |
| ! | TATTCAAGAAATCAATGACT+TGG | - | chr5.4:32231765-32231784 | MS.gene53119:CDS | 25.0% |
| ! | TGAATATGGTGAATATTCTT+TGG | - | chr5.4:32231394-32231413 | MS.gene53119:CDS | 25.0% |
| !! | AGTTTAAGATCATCAACATT+TGG | + | chr5.4:32231515-32231534 | None:intergenic | 25.0% |
| AAAAACTCTTCCAAACTATC+AGG | + | chr5.4:32231854-32231873 | None:intergenic | 30.0% | |
| AATCATTGTCAGAGAAGTTT+GGG | - | chr5.4:32231325-32231344 | MS.gene53119:CDS | 30.0% | |
| AGAGAATTTGAAAAATACGC+TGG | - | chr5.4:32232079-32232098 | MS.gene53119:CDS | 30.0% | |
| TCAACATTAGTTCAGCAAAA+CGG | + | chr5.4:32231733-32231752 | None:intergenic | 30.0% | |
| AAATACGCTGGAGTAGTATT+TGG | - | chr5.4:32232091-32232110 | MS.gene53119:CDS | 35.0% | |
| AACTATCAGGCAGATTTCTT+CGG | + | chr5.4:32231841-32231860 | None:intergenic | 35.0% | |
| AAGAAATCTGCCTGATAGTT+TGG | - | chr5.4:32231841-32231860 | MS.gene53119:CDS | 35.0% | |
| ACGTATCTGTAGTAATCAGA+GGG | + | chr5.4:32231485-32231504 | None:intergenic | 35.0% | |
| CAAACTTCTCTGACAATGAT+TGG | + | chr5.4:32231326-32231345 | None:intergenic | 35.0% | |
| CAATCATTGTCAGAGAAGTT+TGG | - | chr5.4:32231324-32231343 | MS.gene53119:CDS | 35.0% | |
| CTATTAAAGTGAGTCCAAGT+TGG | + | chr5.4:32231541-32231560 | None:intergenic | 35.0% | |
| CTCACTTTAATAGATGGTTG+CGG | - | chr5.4:32231548-32231567 | MS.gene53119:CDS | 35.0% | |
| CTTGGACTCACTTTAATAGA+TGG | - | chr5.4:32231542-32231561 | MS.gene53119:CDS | 35.0% | |
| GATCTTAAACTCATCCAACT+TGG | - | chr5.4:32231524-32231543 | MS.gene53119:CDS | 35.0% | |
| TCTTGGAAATGTTATTCGGA+AGG | - | chr5.4:32231879-32231898 | MS.gene53119:CDS | 35.0% | |
| TTGCCAAATGTAGCGATTAT+CGG | + | chr5.4:32231594-32231613 | None:intergenic | 35.0% | |
| ! | GGAAGAGTTTTTGAACATCT+TGG | - | chr5.4:32231862-32231881 | MS.gene53119:CDS | 35.0% |
| ! | TTTCATGGCGCTTATGATTT+TGG | - | chr5.4:32231794-32231813 | MS.gene53119:CDS | 35.0% |
| AAGTACACTCAATGTGGATC+GGG | - | chr5.4:32231970-32231989 | MS.gene53119:CDS | 40.0% | |
| ACACCGATAATCGCTACATT+TGG | - | chr5.4:32231588-32231607 | MS.gene53119:CDS | 40.0% | |
| ATGGATAACTCCAGGAAACT+CGG | + | chr5.4:32231432-32231451 | None:intergenic | 40.0% | |
| ATGGCAGACGTTTCATAAGT+TGG | - | chr5.4:32232036-32232055 | MS.gene53119:CDS | 40.0% | |
| CAAGTACACTCAATGTGGAT+CGG | - | chr5.4:32231969-32231988 | MS.gene53119:CDS | 40.0% | |
| CATACAATGCATTGCAGTAG+CGG | + | chr5.4:32231931-32231950 | None:intergenic | 40.0% | |
| CGTGATGTTCTTCCTGAATA+TGG | - | chr5.4:32231380-32231399 | MS.gene53119:CDS | 40.0% | |
| GAAGAACATCACGAATGAGA+TGG | + | chr5.4:32231373-32231392 | None:intergenic | 40.0% | |
| GACGTATCTGTAGTAATCAG+AGG | + | chr5.4:32231486-32231505 | None:intergenic | 40.0% | |
| TACTGCAATGCATTGTATGG+TGG | - | chr5.4:32231932-32231951 | MS.gene53119:CDS | 40.0% | |
| TGTAGTAATCAGAGGGTTGA+AGG | + | chr5.4:32231478-32231497 | None:intergenic | 40.0% | |
| TTCAATCGTTGTGTCTCTCA+AGG | - | chr5.4:32231695-32231714 | MS.gene53119:CDS | 40.0% | |
| TTCGGATCAATATCTTCACC+AGG | + | chr5.4:32231823-32231842 | None:intergenic | 40.0% | |
| TTGTCGACGAAGCAAATCAA+TGG | + | chr5.4:32231666-32231685 | None:intergenic | 40.0% | |
| ! | ATTGTATGGTGGTCTTGATC+GGG | - | chr5.4:32231943-32231962 | MS.gene53119:CDS | 40.0% |
| ! | CAATGGAATCCTTGTTGTGA+GGG | + | chr5.4:32231649-32231668 | None:intergenic | 40.0% |
| ! | CATTGTATGGTGGTCTTGAT+CGG | - | chr5.4:32231942-32231961 | MS.gene53119:CDS | 40.0% |
| ! | CGCTTATGATTTTGGATACC+TGG | - | chr5.4:32231802-32231821 | MS.gene53119:CDS | 40.0% |
| ! | TCAATGGAATCCTTGTTGTG+AGG | + | chr5.4:32231650-32231669 | None:intergenic | 40.0% |
| ! | TTTGGTGTATGGATAACTCC+AGG | + | chr5.4:32231440-32231459 | None:intergenic | 40.0% |
| !! | ATGACTTGGGTGACTTTTCA+TGG | - | chr5.4:32231779-32231798 | MS.gene53119:CDS | 40.0% |
| !! | ATTGATTTGCTTCGTCGACA+AGG | - | chr5.4:32231665-32231684 | MS.gene53119:CDS | 40.0% |
| !! | CGATTATCGGTGTCAAAATC+AGG | + | chr5.4:32231581-32231600 | None:intergenic | 40.0% |
| !! | TATCGGTGTCAAAATCAGGA+AGG | + | chr5.4:32231577-32231596 | None:intergenic | 40.0% |
| !! | TTGATTTGCTTCGTCGACAA+GGG | - | chr5.4:32231666-32231685 | MS.gene53119:CDS | 40.0% |
| AGTAATCAGAGGGTTGAAGG+TGG | + | chr5.4:32231475-32231494 | None:intergenic | 45.0% | |
| CGCTACTGCAATGCATTGTA+TGG | - | chr5.4:32231929-32231948 | MS.gene53119:CDS | 45.0% | |
| GCTGAACTAATGTTGACGTC+TGG | - | chr5.4:32231737-32231756 | MS.gene53119:CDS | 45.0% | |
| GGTTGCAAGTACACTCAATG+TGG | - | chr5.4:32231964-32231983 | MS.gene53119:CDS | 45.0% | |
| GTTGGAAGGTCTCATCAATC+CGG | - | chr5.4:32231995-32232014 | MS.gene53119:CDS | 45.0% | |
| !! | GATGGTCGACTTTTGGTGTA+TGG | + | chr5.4:32231451-32231470 | None:intergenic | 45.0% |
| CTGCCATGTCAACAAGCTGT+CGG | + | chr5.4:32232023-32232042 | None:intergenic | 50.0% | |
| TCGATAGACACCGAGTTTCC+TGG | - | chr5.4:32231419-32231438 | MS.gene53119:CDS | 50.0% | |
| TGTCAACAAGCTGTCGGATC+CGG | + | chr5.4:32232017-32232036 | None:intergenic | 50.0% | |
| ! | ATGTGGATCGGGTTGTTGGA+AGG | - | chr5.4:32231981-32232000 | MS.gene53119:CDS | 50.0% |
| ! | CTCAATGTGGATCGGGTTGT+TGG | - | chr5.4:32231977-32231996 | MS.gene53119:CDS | 50.0% |
| !! | GATCCGACAGCTTGTTGACA+TGG | - | chr5.4:32232017-32232036 | MS.gene53119:CDS | 50.0% |
| TGAGCGTGACCCTCACAACA+AGG | - | chr5.4:32231637-32231656 | MS.gene53119:CDS | 55.0% | |
| !! | AGGTGGCGATGGTCGACTTT+TGG | + | chr5.4:32231458-32231477 | None:intergenic | 55.0% |
| CAGAGGGTTGAAGGTGGCGA+TGG | + | chr5.4:32231469-32231488 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 32231284 | 32232132 | 32231284 | ID=MS.gene53119 |
| chr5.4 | mRNA | 32231284 | 32232132 | 32231284 | ID=MS.gene53119.t1;Parent=MS.gene53119 |
| chr5.4 | exon | 32231284 | 32232132 | 32231284 | ID=MS.gene53119.t1.exon1;Parent=MS.gene53119.t1 |
| chr5.4 | CDS | 32231284 | 32232132 | 32231284 | ID=cds.MS.gene53119.t1;Parent=MS.gene53119.t1 |
| Gene Sequence |
| Protein sequence |
