Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53120.t1 | XP_024639118.1 | 90.8 | 282 | 26 | 0 | 1 | 282 | 1 | 282 | 5.40E-149 | 537 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53120.t1 | Q9FMS6 | 56.6 | 274 | 113 | 3 | 10 | 280 | 7 | 277 | 2.6e-83 | 310.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53120.t1 | A0A396HQ65 | 90.8 | 282 | 26 | 0 | 1 | 282 | 1 | 282 | 3.9e-149 | 537.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53120 | MS.gene025628 | PPI |
| MS.gene53120 | MS.gene034816 | PPI |
| MS.gene53120 | MS.gene72978 | PPI |
| MS.gene53120 | MS.gene41100 | PPI |
| MS.gene53120 | MS.gene41097 | PPI |
| MS.gene53120 | MS.gene000609 | PPI |
| MS.gene53120 | MS.gene21541 | PPI |
| MS.gene053225 | MS.gene53120 | PPI |
| MS.gene53120 | MS.gene007154 | PPI |
| MS.gene53120 | MS.gene41101 | PPI |
| MS.gene034815 | MS.gene53120 | PPI |
| MS.gene53120 | MS.gene065708 | PPI |
| MS.gene025629 | MS.gene53120 | PPI |
| MS.gene034250 | MS.gene53120 | PPI |
| MS.gene53120 | MS.gene065709 | PPI |
| MS.gene53120 | MS.gene72977 | PPI |
| MS.gene53120 | MS.gene065707 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53120.t1 | AT5G22250 | 56.569 | 274 | 113 | 3 | 10 | 280 | 7 | 277 | 9.08e-106 | 309 |
| MS.gene53120.t1 | AT3G44260 | 55.390 | 269 | 111 | 5 | 15 | 280 | 17 | 279 | 1.76e-98 | 290 |
| MS.gene53120.t1 | AT5G10960 | 47.080 | 274 | 134 | 6 | 7 | 280 | 5 | 267 | 5.00e-80 | 243 |
| MS.gene53120.t1 | AT2G32070 | 47.940 | 267 | 130 | 6 | 15 | 280 | 10 | 268 | 7.03e-79 | 240 |
| MS.gene53120.t1 | AT1G80780 | 45.865 | 266 | 136 | 5 | 15 | 280 | 10 | 267 | 2.01e-74 | 229 |
| MS.gene53120.t1 | AT1G80780 | 45.865 | 266 | 136 | 5 | 15 | 280 | 10 | 267 | 2.01e-74 | 229 |
| MS.gene53120.t1 | AT1G80780 | 45.865 | 266 | 136 | 5 | 15 | 280 | 10 | 267 | 2.80e-74 | 229 |
| MS.gene53120.t1 | AT1G15920 | 44.604 | 278 | 144 | 6 | 9 | 282 | 8 | 279 | 2.65e-70 | 219 |
| MS.gene53120.t1 | AT1G15920 | 44.604 | 278 | 144 | 6 | 9 | 282 | 8 | 279 | 2.65e-70 | 219 |
| MS.gene53120.t1 | AT1G61470 | 39.024 | 246 | 125 | 8 | 19 | 259 | 4 | 229 | 1.06e-42 | 147 |
| MS.gene53120.t1 | AT1G06450 | 34.559 | 272 | 150 | 10 | 16 | 281 | 8 | 257 | 1.45e-37 | 136 |
| MS.gene53120.t1 | AT1G27820 | 36.614 | 254 | 134 | 9 | 13 | 259 | 2 | 235 | 1.99e-36 | 132 |
| MS.gene53120.t1 | AT1G27890 | 37.652 | 247 | 129 | 9 | 19 | 259 | 3 | 230 | 3.80e-35 | 128 |
| MS.gene53120.t1 | AT3G44240 | 35.622 | 233 | 129 | 8 | 31 | 260 | 3 | 217 | 1.95e-33 | 122 |
Find 57 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATGGCGCTTATGATTT+TGG | 0.117389 | 5.4:-32244104 | MS.gene53120:CDS |
| AAATACGCTGGAGTAGTATT+TGG | 0.224745 | 5.4:-32243807 | MS.gene53120:CDS |
| TGAATATGGTGAATATTCTT+TGG | 0.278766 | 5.4:-32244504 | MS.gene53120:CDS |
| CGTGATGTTCTTCCTGAATA+TGG | 0.278966 | 5.4:-32244518 | MS.gene53120:CDS |
| CAATCATTGTCCGAGAAGTT+TGG | 0.285300 | 5.4:-32244574 | MS.gene53120:CDS |
| CTTGGACTCACTTTAATAGA+TGG | 0.314324 | 5.4:-32244356 | MS.gene53120:CDS |
| AAGAAATCTGCCTGATAGTT+TGG | 0.321613 | 5.4:-32244057 | MS.gene53120:CDS |
| CAAACTTCTCGGACAATGAT+TGG | 0.324520 | 5.4:+32244575 | None:intergenic |
| TCGATAGACACCGAGTTTCC+TGG | 0.362514 | 5.4:-32244479 | MS.gene53120:CDS |
| AGTTTAAGATCATCCACATT+AGG | 0.373743 | 5.4:+32244386 | None:intergenic |
| AATCATTGTCCGAGAAGTTT+GGG | 0.374317 | 5.4:-32244573 | MS.gene53120:CDS |
| CGATTATCGGTGTCAAAATC+AGG | 0.397384 | 5.4:+32244320 | None:intergenic |
| ACACCGATAATCGCTACATT+TGG | 0.399135 | 5.4:-32244310 | MS.gene53120:CDS |
| AGGTGGCGATGGTCGACTTT+TGG | 0.407532 | 5.4:+32244443 | None:intergenic |
| CTCAATGTGGATCGGGTTGT+TGG | 0.408633 | 5.4:-32243921 | MS.gene53120:CDS |
| TTGCCAAATGTAGCGATTAT+CGG | 0.410093 | 5.4:+32244307 | None:intergenic |
| AAAGAATATTCACCATATTC+AGG | 0.416250 | 5.4:+32244506 | None:intergenic |
| CATTGTATGGTGGTCTTGAT+CGG | 0.438293 | 5.4:-32243956 | MS.gene53120:CDS |
| AACTATCAGGCAGATTTCTT+CGG | 0.444161 | 5.4:+32244060 | None:intergenic |
| TGTCAACAAGCTGTCGGATC+CGG | 0.457649 | 5.4:+32243884 | None:intergenic |
| AGTTGTGTGCCCAAACTTCT+CGG | 0.466420 | 5.4:+32244564 | None:intergenic |
| TATTCAAGAAATCAATGACT+TGG | 0.469568 | 5.4:-32244133 | MS.gene53120:CDS |
| CGCTACTGCAATGCATTGTA+TGG | 0.477422 | 5.4:-32243969 | MS.gene53120:CDS |
| ATGGCAGACGTTTCATAAGT+TGG | 0.479969 | 5.4:-32243862 | MS.gene53120:CDS |
| TGTAGTAATCAGAGGGTTGA+AGG | 0.480186 | 5.4:+32244423 | None:intergenic |
| GCTGAACTAATGTTGACGTC+TGG | 0.481099 | 5.4:-32244161 | MS.gene53120:CDS |
| ATTGTATGGTGGTCTTGATC+GGG | 0.514058 | 5.4:-32243955 | MS.gene53120:CDS |
| AAGTACACTCAATGTGGATC+GGG | 0.524067 | 5.4:-32243928 | MS.gene53120:CDS |
| CAATGGACTCTTTGTTGTGA+GGG | 0.524178 | 5.4:+32244252 | None:intergenic |
| TTGTCGACGAAGCAAATCAA+TGG | 0.528289 | 5.4:+32244235 | None:intergenic |
| TTTGGTGCATGGATAACTCC+AGG | 0.535086 | 5.4:+32244461 | None:intergenic |
| TTCAATCGTTGTGTCTCTCA+AGG | 0.540052 | 5.4:-32244203 | MS.gene53120:CDS |
| GTTGGAAGGTCTCATCAATC+CGG | 0.540408 | 5.4:-32243903 | MS.gene53120:CDS |
| ATGTGGATCGGGTTGTTGGA+AGG | 0.540595 | 5.4:-32243917 | MS.gene53120:CDS |
| ATTCAAGAAATCAATGACTT+GGG | 0.542864 | 5.4:-32244132 | MS.gene53120:CDS |
| GATCTTAAACTCATCCAACT+TGG | 0.545194 | 5.4:-32244374 | MS.gene53120:CDS |
| CAAGTACACTCAATGTGGAT+CGG | 0.548190 | 5.4:-32243929 | MS.gene53120:CDS |
| CTATTAAAGTGAGTCCAAGT+TGG | 0.558411 | 5.4:+32244360 | None:intergenic |
| CAGAGGGTTGAAGGTGGCGA+TGG | 0.559485 | 5.4:+32244432 | None:intergenic |
| TTCGGATCAATATCTTCACC+AGG | 0.564436 | 5.4:+32244078 | None:intergenic |
| GAAGAACATCACGAATGAGA+TGG | 0.565749 | 5.4:+32244528 | None:intergenic |
| ATTGATTTGCTTCGTCGACA+AGG | 0.569126 | 5.4:-32244233 | MS.gene53120:CDS |
| TCAACATTAGTTCAGCAAAA+CGG | 0.571611 | 5.4:+32244168 | None:intergenic |
| GATCCGACAGCTTGTTGACA+TGG | 0.577163 | 5.4:-32243881 | MS.gene53120:CDS |
| CACGTATCTGTAGTAATCAG+AGG | 0.581646 | 5.4:+32244415 | None:intergenic |
| CATACAATGCATTGCAGTAG+CGG | 0.583860 | 5.4:+32243970 | None:intergenic |
| TTGATTTGCTTCGTCGACAA+GGG | 0.585778 | 5.4:-32244232 | MS.gene53120:CDS |
| ATGGATAACTCCAGGAAACT+CGG | 0.585861 | 5.4:+32244469 | None:intergenic |
| TCAATGGACTCTTTGTTGTG+AGG | 0.592655 | 5.4:+32244251 | None:intergenic |
| TACTGCAATGCATTGTATGG+TGG | 0.597106 | 5.4:-32243966 | MS.gene53120:CDS |
| CTCACTTTAATAGATGGTTG+CGG | 0.607202 | 5.4:-32244350 | MS.gene53120:CDS |
| CTGCCATGTCAACAAGCTGT+CGG | 0.613501 | 5.4:+32243878 | None:intergenic |
| ACGTATCTGTAGTAATCAGA+GGG | 0.642679 | 5.4:+32244416 | None:intergenic |
| ATACGTGTTGAAGCCTAATG+TGG | 0.647752 | 5.4:-32244399 | MS.gene53120:CDS |
| GGTTGCAAGTACACTCAATG+TGG | 0.665808 | 5.4:-32243934 | MS.gene53120:CDS |
| TATCGGTGTCAAAATCAGGA+AGG | 0.683593 | 5.4:+32244324 | None:intergenic |
| AGTAATCAGAGGGTTGAAGG+TGG | 0.690515 | 5.4:+32244426 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTTGGAAATGTTATT+CGG | - | chr5.4:32244379-32244398 | MS.gene53120:CDS | 20.0% |
| ! | AAAGAATATTCACCATATTC+AGG | + | chr5.4:32243899-32243918 | None:intergenic | 25.0% |
| ! | AATGAATTTGAAAAATACGC+TGG | - | chr5.4:32244583-32244602 | MS.gene53120:CDS | 25.0% |
| ! | ATTCAAGAAATCAATGACTT+GGG | - | chr5.4:32244270-32244289 | MS.gene53120:CDS | 25.0% |
| ! | TATTCAAGAAATCAATGACT+TGG | - | chr5.4:32244269-32244288 | MS.gene53120:CDS | 25.0% |
| ! | TGAATATGGTGAATATTCTT+TGG | - | chr5.4:32243898-32243917 | MS.gene53120:CDS | 25.0% |
| AAAAACTCTTCCAAACTATC+AGG | + | chr5.4:32244358-32244377 | None:intergenic | 30.0% | |
| TCAACATTAGTTCAGCAAAA+CGG | + | chr5.4:32244237-32244256 | None:intergenic | 30.0% | |
| ! | AGTTTAAGATCATCCACATT+AGG | + | chr5.4:32244019-32244038 | None:intergenic | 30.0% |
| ! | GGAAGAGTTTTTGAACATTT+TGG | - | chr5.4:32244366-32244385 | MS.gene53120:CDS | 30.0% |
| !! | TTTTGGAAATGTTATTCGGA+AGG | - | chr5.4:32244383-32244402 | MS.gene53120:CDS | 30.0% |
| AAATACGCTGGAGTAGTATT+TGG | - | chr5.4:32244595-32244614 | MS.gene53120:CDS | 35.0% | |
| AACTATCAGGCAGATTTCTT+CGG | + | chr5.4:32244345-32244364 | None:intergenic | 35.0% | |
| AAGAAATCTGCCTGATAGTT+TGG | - | chr5.4:32244345-32244364 | MS.gene53120:CDS | 35.0% | |
| AATCATTGTCCGAGAAGTTT+GGG | - | chr5.4:32243829-32243848 | MS.gene53120:CDS | 35.0% | |
| ACGTATCTGTAGTAATCAGA+GGG | + | chr5.4:32243989-32244008 | None:intergenic | 35.0% | |
| CTATTAAAGTGAGTCCAAGT+TGG | + | chr5.4:32244045-32244064 | None:intergenic | 35.0% | |
| CTCACTTTAATAGATGGTTG+CGG | - | chr5.4:32244052-32244071 | MS.gene53120:CDS | 35.0% | |
| CTTGGACTCACTTTAATAGA+TGG | - | chr5.4:32244046-32244065 | MS.gene53120:CDS | 35.0% | |
| GATCTTAAACTCATCCAACT+TGG | - | chr5.4:32244028-32244047 | MS.gene53120:CDS | 35.0% | |
| TTGCCAAATGTAGCGATTAT+CGG | + | chr5.4:32244098-32244117 | None:intergenic | 35.0% | |
| ! | TTTCATGGCGCTTATGATTT+TGG | - | chr5.4:32244298-32244317 | MS.gene53120:CDS | 35.0% |
| AAGTACACTCAATGTGGATC+GGG | - | chr5.4:32244474-32244493 | MS.gene53120:CDS | 40.0% | |
| ACACCGATAATCGCTACATT+TGG | - | chr5.4:32244092-32244111 | MS.gene53120:CDS | 40.0% | |
| ATACGTGTTGAAGCCTAATG+TGG | - | chr5.4:32244003-32244022 | MS.gene53120:CDS | 40.0% | |
| ATGGATAACTCCAGGAAACT+CGG | + | chr5.4:32243936-32243955 | None:intergenic | 40.0% | |
| ATGGCAGACGTTTCATAAGT+TGG | - | chr5.4:32244540-32244559 | MS.gene53120:CDS | 40.0% | |
| CAAACTTCTCGGACAATGAT+TGG | + | chr5.4:32243830-32243849 | None:intergenic | 40.0% | |
| CAAGTACACTCAATGTGGAT+CGG | - | chr5.4:32244473-32244492 | MS.gene53120:CDS | 40.0% | |
| CAATCATTGTCCGAGAAGTT+TGG | - | chr5.4:32243828-32243847 | MS.gene53120:CDS | 40.0% | |
| CACGTATCTGTAGTAATCAG+AGG | + | chr5.4:32243990-32244009 | None:intergenic | 40.0% | |
| CATACAATGCATTGCAGTAG+CGG | + | chr5.4:32244435-32244454 | None:intergenic | 40.0% | |
| CGTGATGTTCTTCCTGAATA+TGG | - | chr5.4:32243884-32243903 | MS.gene53120:CDS | 40.0% | |
| GAAGAACATCACGAATGAGA+TGG | + | chr5.4:32243877-32243896 | None:intergenic | 40.0% | |
| TACTGCAATGCATTGTATGG+TGG | - | chr5.4:32244436-32244455 | MS.gene53120:CDS | 40.0% | |
| TGTAGTAATCAGAGGGTTGA+AGG | + | chr5.4:32243982-32244001 | None:intergenic | 40.0% | |
| TTCAATCGTTGTGTCTCTCA+AGG | - | chr5.4:32244199-32244218 | MS.gene53120:CDS | 40.0% | |
| TTCGGATCAATATCTTCACC+AGG | + | chr5.4:32244327-32244346 | None:intergenic | 40.0% | |
| TTGTCGACGAAGCAAATCAA+TGG | + | chr5.4:32244170-32244189 | None:intergenic | 40.0% | |
| ! | ATTGTATGGTGGTCTTGATC+GGG | - | chr5.4:32244447-32244466 | MS.gene53120:CDS | 40.0% |
| ! | CAATGGACTCTTTGTTGTGA+GGG | + | chr5.4:32244153-32244172 | None:intergenic | 40.0% |
| ! | CATTGTATGGTGGTCTTGAT+CGG | - | chr5.4:32244446-32244465 | MS.gene53120:CDS | 40.0% |
| ! | CGCTTATGATTTTGGATACC+TGG | - | chr5.4:32244306-32244325 | MS.gene53120:CDS | 40.0% |
| ! | TCAATGGACTCTTTGTTGTG+AGG | + | chr5.4:32244154-32244173 | None:intergenic | 40.0% |
| !! | ATGACTTGGGTGACTTTTCA+TGG | - | chr5.4:32244283-32244302 | MS.gene53120:CDS | 40.0% |
| !! | ATTGATTTGCTTCGTCGACA+AGG | - | chr5.4:32244169-32244188 | MS.gene53120:CDS | 40.0% |
| !! | CGATTATCGGTGTCAAAATC+AGG | + | chr5.4:32244085-32244104 | None:intergenic | 40.0% |
| !! | TATCGGTGTCAAAATCAGGA+AGG | + | chr5.4:32244081-32244100 | None:intergenic | 40.0% |
| !! | TTGATTTGCTTCGTCGACAA+GGG | - | chr5.4:32244170-32244189 | MS.gene53120:CDS | 40.0% |
| AGTAATCAGAGGGTTGAAGG+TGG | + | chr5.4:32243979-32243998 | None:intergenic | 45.0% | |
| AGTTGTGTGCCCAAACTTCT+CGG | + | chr5.4:32243841-32243860 | None:intergenic | 45.0% | |
| CGCTACTGCAATGCATTGTA+TGG | - | chr5.4:32244433-32244452 | MS.gene53120:CDS | 45.0% | |
| GCTGAACTAATGTTGACGTC+TGG | - | chr5.4:32244241-32244260 | MS.gene53120:CDS | 45.0% | |
| GGTTGCAAGTACACTCAATG+TGG | - | chr5.4:32244468-32244487 | MS.gene53120:CDS | 45.0% | |
| GTTGGAAGGTCTCATCAATC+CGG | - | chr5.4:32244499-32244518 | MS.gene53120:CDS | 45.0% | |
| ! | TTTGGTGCATGGATAACTCC+AGG | + | chr5.4:32243944-32243963 | None:intergenic | 45.0% |
| CTGCCATGTCAACAAGCTGT+CGG | + | chr5.4:32244527-32244546 | None:intergenic | 50.0% | |
| TCGATAGACACCGAGTTTCC+TGG | - | chr5.4:32243923-32243942 | MS.gene53120:CDS | 50.0% | |
| TGTCAACAAGCTGTCGGATC+CGG | + | chr5.4:32244521-32244540 | None:intergenic | 50.0% | |
| ! | ATGTGGATCGGGTTGTTGGA+AGG | - | chr5.4:32244485-32244504 | MS.gene53120:CDS | 50.0% |
| ! | CTCAATGTGGATCGGGTTGT+TGG | - | chr5.4:32244481-32244500 | MS.gene53120:CDS | 50.0% |
| !! | GATCCGACAGCTTGTTGACA+TGG | - | chr5.4:32244521-32244540 | MS.gene53120:CDS | 50.0% |
| !! | GATGGTCGACTTTTGGTGCA+TGG | + | chr5.4:32243955-32243974 | None:intergenic | 50.0% |
| !! | AGGTGGCGATGGTCGACTTT+TGG | + | chr5.4:32243962-32243981 | None:intergenic | 55.0% |
| CAGAGGGTTGAAGGTGGCGA+TGG | + | chr5.4:32243973-32243992 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 32243788 | 32244636 | 32243788 | ID=MS.gene53120 |
| chr5.4 | mRNA | 32243788 | 32244636 | 32243788 | ID=MS.gene53120.t1;Parent=MS.gene53120 |
| chr5.4 | exon | 32243788 | 32244636 | 32243788 | ID=MS.gene53120.t1.exon1;Parent=MS.gene53120.t1 |
| chr5.4 | CDS | 32243788 | 32244636 | 32243788 | ID=cds.MS.gene53120.t1;Parent=MS.gene53120.t1 |
| Gene Sequence |
| Protein sequence |
