Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53123.t1 | XP_024639118.1 | 90.4 | 282 | 27 | 0 | 1 | 282 | 1 | 282 | 1.10E-146 | 529.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53123.t1 | Q9FMS6 | 56.6 | 274 | 113 | 3 | 10 | 280 | 7 | 277 | 3.4e-83 | 309.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53123.t1 | A0A396HQ65 | 90.4 | 282 | 27 | 0 | 1 | 282 | 1 | 282 | 8.1e-147 | 529.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene41100 | MS.gene53123 | PPI |
| MS.gene000609 | MS.gene53123 | PPI |
| MS.gene53123 | MS.gene21541 | PPI |
| MS.gene53123 | MS.gene41097 | PPI |
| MS.gene065707 | MS.gene53123 | PPI |
| MS.gene41101 | MS.gene53123 | PPI |
| MS.gene065708 | MS.gene53123 | PPI |
| MS.gene053225 | MS.gene53123 | PPI |
| MS.gene034815 | MS.gene53123 | PPI |
| MS.gene025629 | MS.gene53123 | PPI |
| MS.gene034250 | MS.gene53123 | PPI |
| MS.gene034816 | MS.gene53123 | PPI |
| MS.gene72977 | MS.gene53123 | PPI |
| MS.gene065709 | MS.gene53123 | PPI |
| MS.gene72978 | MS.gene53123 | PPI |
| MS.gene53123 | MS.gene025628 | PPI |
| MS.gene007154 | MS.gene53123 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53123.t1 | AT5G22250 | 56.569 | 274 | 113 | 3 | 10 | 280 | 7 | 277 | 7.54e-106 | 309 |
| MS.gene53123.t1 | AT3G44260 | 55.390 | 269 | 111 | 5 | 15 | 280 | 17 | 279 | 2.66e-98 | 290 |
| MS.gene53123.t1 | AT5G10960 | 47.445 | 274 | 133 | 6 | 7 | 280 | 5 | 267 | 1.61e-80 | 244 |
| MS.gene53123.t1 | AT2G32070 | 48.315 | 267 | 129 | 6 | 15 | 280 | 10 | 268 | 1.91e-79 | 242 |
| MS.gene53123.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 3.39e-75 | 231 |
| MS.gene53123.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 3.39e-75 | 231 |
| MS.gene53123.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 5.92e-75 | 231 |
| MS.gene53123.t1 | AT1G15920 | 45.324 | 278 | 142 | 6 | 9 | 282 | 8 | 279 | 2.56e-72 | 224 |
| MS.gene53123.t1 | AT1G15920 | 45.324 | 278 | 142 | 6 | 9 | 282 | 8 | 279 | 2.56e-72 | 224 |
| MS.gene53123.t1 | AT1G61470 | 39.024 | 246 | 125 | 8 | 19 | 259 | 4 | 229 | 5.34e-43 | 148 |
| MS.gene53123.t1 | AT1G06450 | 34.926 | 272 | 149 | 10 | 16 | 281 | 8 | 257 | 1.21e-38 | 139 |
| MS.gene53123.t1 | AT1G27820 | 36.614 | 254 | 134 | 9 | 13 | 259 | 2 | 235 | 1.04e-36 | 133 |
| MS.gene53123.t1 | AT1G27890 | 37.652 | 247 | 129 | 9 | 19 | 259 | 3 | 230 | 2.18e-35 | 129 |
| MS.gene53123.t1 | AT3G44240 | 35.622 | 233 | 129 | 8 | 31 | 260 | 3 | 217 | 7.06e-34 | 123 |
Find 56 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATGGCGCTTATGATTT+TGG | 0.117389 | 5.4:-32304175 | MS.gene53123:CDS |
| AAATACGCTGGAGTAGTATT+TGG | 0.224745 | 5.4:-32303878 | MS.gene53123:CDS |
| TGAATATGGTGAATATTCTT+TGG | 0.278766 | 5.4:-32304575 | MS.gene53123:CDS |
| CGTGATGTTCTTCCTGAATA+TGG | 0.278966 | 5.4:-32304589 | MS.gene53123:CDS |
| CAATCATTGTCCGAGAAGTT+TGG | 0.285300 | 5.4:-32304645 | MS.gene53123:CDS |
| CTTGGACTCACTTTAATAGA+TGG | 0.314324 | 5.4:-32304427 | MS.gene53123:CDS |
| AAGAAATCTGCCTGATAGTT+TGG | 0.321613 | 5.4:-32304128 | MS.gene53123:CDS |
| CAAACTTCTCGGACAATGAT+TGG | 0.324520 | 5.4:+32304646 | None:intergenic |
| AGTTTAAGATCATCAACATT+AGG | 0.373743 | 5.4:+32304457 | None:intergenic |
| AATCATTGTCCGAGAAGTTT+GGG | 0.374317 | 5.4:-32304644 | MS.gene53123:CDS |
| AATGTGTTTGCACCAACTTA+TGG | 0.396776 | 5.4:+32303921 | None:intergenic |
| ACACAGATAATCGCTACATT+TGG | 0.408173 | 5.4:-32304381 | MS.gene53123:CDS |
| CTCAATGTGGATCGGGTTGT+TGG | 0.408633 | 5.4:-32303992 | MS.gene53123:CDS |
| AAAGAATATTCACCATATTC+AGG | 0.416250 | 5.4:+32304577 | None:intergenic |
| CGATTATCTGTGTCAAAATC+AGG | 0.420663 | 5.4:+32304391 | None:intergenic |
| AGGTGGCGATGGTCGACTTT+TGG | 0.420934 | 5.4:+32304514 | None:intergenic |
| TCGATAGACACTGAATTTCC+TGG | 0.427087 | 5.4:-32304550 | MS.gene53123:CDS |
| CATTGTATGGTGGTCTTGAT+CGG | 0.438293 | 5.4:-32304027 | MS.gene53123:CDS |
| AACTATCAGGCAGATTTCTT+CGG | 0.444161 | 5.4:+32304131 | None:intergenic |
| CAATGGACTCTTTGTTCTGA+GGG | 0.467682 | 5.4:+32304323 | None:intergenic |
| AGTTATGTGCCCAAACTTCT+CGG | 0.468860 | 5.4:+32304635 | None:intergenic |
| TATTCAAGAAATCAATGACT+TGG | 0.469568 | 5.4:-32304204 | MS.gene53123:CDS |
| CGCTACTGCAATGCATTGTA+TGG | 0.477422 | 5.4:-32304040 | MS.gene53123:CDS |
| TGTCAACAAGCTATCGGATC+CGG | 0.479582 | 5.4:+32303955 | None:intergenic |
| TGTAGTAATCAGAGGGTTGA+AGG | 0.480186 | 5.4:+32304494 | None:intergenic |
| GCTGAACTAATGTTGACGTC+TGG | 0.481099 | 5.4:-32304232 | MS.gene53123:CDS |
| ATTGTATGGTGGTCTTGATC+GGG | 0.514058 | 5.4:-32304026 | MS.gene53123:CDS |
| AAGTACACTCAATGTGGATC+GGG | 0.524067 | 5.4:-32303999 | MS.gene53123:CDS |
| TTGTCGACGAAGCAAATCAA+TGG | 0.528289 | 5.4:+32304306 | None:intergenic |
| TCAATGGACTCTTTGTTCTG+AGG | 0.533732 | 5.4:+32304322 | None:intergenic |
| TTCAATCGTTGTGTCTCTCA+AGG | 0.540052 | 5.4:-32304274 | MS.gene53123:CDS |
| GTTGGAAGGTCTCATCAATC+CGG | 0.540408 | 5.4:-32303974 | MS.gene53123:CDS |
| ATGTGGATCGGGTTGTTGGA+AGG | 0.540595 | 5.4:-32303988 | MS.gene53123:CDS |
| ATTCAAGAAATCAATGACTT+GGG | 0.542864 | 5.4:-32304203 | MS.gene53123:CDS |
| GATCTTAAACTCATCCAACT+TGG | 0.545194 | 5.4:-32304445 | MS.gene53123:CDS |
| CAAGTACACTCAATGTGGAT+CGG | 0.548190 | 5.4:-32304000 | MS.gene53123:CDS |
| CTGCCATGTCAACAAGCTAT+CGG | 0.556385 | 5.4:+32303949 | None:intergenic |
| CTATTAAAGTGAGTCCAAGT+TGG | 0.558411 | 5.4:+32304431 | None:intergenic |
| TTTGGTGTATGGATAACTCC+AGG | 0.559457 | 5.4:+32304532 | None:intergenic |
| CAGAGGGTTGAAGGTGGCGA+TGG | 0.559485 | 5.4:+32304503 | None:intergenic |
| ATGGCAGACGTTCCATAAGT+TGG | 0.560131 | 5.4:-32303933 | MS.gene53123:CDS |
| CACGCTCGACGTTGAAGTCA+CGG | 0.560577 | 5.4:+32304347 | None:intergenic |
| TTCGGATCAATATCTTCACC+AGG | 0.564436 | 5.4:+32304149 | None:intergenic |
| GAAGAACATCACGAATGAGA+TGG | 0.565749 | 5.4:+32304599 | None:intergenic |
| ATTGATTTGCTTCGTCGACA+AGG | 0.569126 | 5.4:-32304304 | MS.gene53123:CDS |
| TCAACATTAGTTCAGCAAAA+CGG | 0.571611 | 5.4:+32304239 | None:intergenic |
| GATCCGATAGCTTGTTGACA+TGG | 0.582907 | 5.4:-32303952 | MS.gene53123:CDS |
| CATACAATGCATTGCAGTAG+CGG | 0.583860 | 5.4:+32304041 | None:intergenic |
| TTGATTTGCTTCGTCGACAA+GGG | 0.585778 | 5.4:-32304303 | MS.gene53123:CDS |
| GACGTATCTGTAGTAATCAG+AGG | 0.593946 | 5.4:+32304486 | None:intergenic |
| TACTGCAATGCATTGTATGG+TGG | 0.597106 | 5.4:-32304037 | MS.gene53123:CDS |
| CTCACTTTAATAGATGGTTG+CGG | 0.607202 | 5.4:-32304421 | MS.gene53123:CDS |
| ACGTATCTGTAGTAATCAGA+GGG | 0.626051 | 5.4:+32304487 | None:intergenic |
| GGTTGCAAGTACACTCAATG+TGG | 0.665808 | 5.4:-32304005 | MS.gene53123:CDS |
| TATCTGTGTCAAAATCAGGA+AGG | 0.673620 | 5.4:+32304395 | None:intergenic |
| AGTAATCAGAGGGTTGAAGG+TGG | 0.690515 | 5.4:+32304497 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTTGGAAAAATTATT+CGG | - | chr5.4:32304450-32304469 | MS.gene53123:CDS | 15.0% |
| ! | AAAGAATATTCACCATATTC+AGG | + | chr5.4:32303970-32303989 | None:intergenic | 25.0% |
| ! | AATGAATTTGAAAAATACGC+TGG | - | chr5.4:32304654-32304673 | MS.gene53123:CDS | 25.0% |
| ! | ATTCAAGAAATCAATGACTT+GGG | - | chr5.4:32304341-32304360 | MS.gene53123:CDS | 25.0% |
| ! | TATTCAAGAAATCAATGACT+TGG | - | chr5.4:32304340-32304359 | MS.gene53123:CDS | 25.0% |
| ! | TGAATATGGTGAATATTCTT+TGG | - | chr5.4:32303969-32303988 | MS.gene53123:CDS | 25.0% |
| !! | AGTTTAAGATCATCAACATT+AGG | + | chr5.4:32304090-32304109 | None:intergenic | 25.0% |
| !!! | TTTTGGAAAAATTATTCGGA+AGG | - | chr5.4:32304454-32304473 | MS.gene53123:CDS | 25.0% |
| AAAAACTCTTCCAAACTATC+AGG | + | chr5.4:32304429-32304448 | None:intergenic | 30.0% | |
| TCAACATTAGTTCAGCAAAA+CGG | + | chr5.4:32304308-32304327 | None:intergenic | 30.0% | |
| ! | GGAAGAGTTTTTGAACATTT+TGG | - | chr5.4:32304437-32304456 | MS.gene53123:CDS | 30.0% |
| AAATACGCTGGAGTAGTATT+TGG | - | chr5.4:32304666-32304685 | MS.gene53123:CDS | 35.0% | |
| AACTATCAGGCAGATTTCTT+CGG | + | chr5.4:32304416-32304435 | None:intergenic | 35.0% | |
| AAGAAATCTGCCTGATAGTT+TGG | - | chr5.4:32304416-32304435 | MS.gene53123:CDS | 35.0% | |
| AATCATTGTCCGAGAAGTTT+GGG | - | chr5.4:32303900-32303919 | MS.gene53123:CDS | 35.0% | |
| AATGTGTTTGCACCAACTTA+TGG | + | chr5.4:32304626-32304645 | None:intergenic | 35.0% | |
| ACACAGATAATCGCTACATT+TGG | - | chr5.4:32304163-32304182 | MS.gene53123:CDS | 35.0% | |
| ACGTATCTGTAGTAATCAGA+GGG | + | chr5.4:32304060-32304079 | None:intergenic | 35.0% | |
| CGATTATCTGTGTCAAAATC+AGG | + | chr5.4:32304156-32304175 | None:intergenic | 35.0% | |
| CTATTAAAGTGAGTCCAAGT+TGG | + | chr5.4:32304116-32304135 | None:intergenic | 35.0% | |
| CTCACTTTAATAGATGGTTG+CGG | - | chr5.4:32304123-32304142 | MS.gene53123:CDS | 35.0% | |
| CTTGGACTCACTTTAATAGA+TGG | - | chr5.4:32304117-32304136 | MS.gene53123:CDS | 35.0% | |
| GATCTTAAACTCATCCAACT+TGG | - | chr5.4:32304099-32304118 | MS.gene53123:CDS | 35.0% | |
| TATCTGTGTCAAAATCAGGA+AGG | + | chr5.4:32304152-32304171 | None:intergenic | 35.0% | |
| ! | TTTCATGGCGCTTATGATTT+TGG | - | chr5.4:32304369-32304388 | MS.gene53123:CDS | 35.0% |
| AAGTACACTCAATGTGGATC+GGG | - | chr5.4:32304545-32304564 | MS.gene53123:CDS | 40.0% | |
| AGTTATGTGCCCAAACTTCT+CGG | + | chr5.4:32303912-32303931 | None:intergenic | 40.0% | |
| CAAACTTCTCGGACAATGAT+TGG | + | chr5.4:32303901-32303920 | None:intergenic | 40.0% | |
| CAAGTACACTCAATGTGGAT+CGG | - | chr5.4:32304544-32304563 | MS.gene53123:CDS | 40.0% | |
| CAATCATTGTCCGAGAAGTT+TGG | - | chr5.4:32303899-32303918 | MS.gene53123:CDS | 40.0% | |
| CATACAATGCATTGCAGTAG+CGG | + | chr5.4:32304506-32304525 | None:intergenic | 40.0% | |
| CGTGATGTTCTTCCTGAATA+TGG | - | chr5.4:32303955-32303974 | MS.gene53123:CDS | 40.0% | |
| GAAGAACATCACGAATGAGA+TGG | + | chr5.4:32303948-32303967 | None:intergenic | 40.0% | |
| GACGTATCTGTAGTAATCAG+AGG | + | chr5.4:32304061-32304080 | None:intergenic | 40.0% | |
| TACTGCAATGCATTGTATGG+TGG | - | chr5.4:32304507-32304526 | MS.gene53123:CDS | 40.0% | |
| TCGATAGACACTGAATTTCC+TGG | - | chr5.4:32303994-32304013 | MS.gene53123:CDS | 40.0% | |
| TGTAGTAATCAGAGGGTTGA+AGG | + | chr5.4:32304053-32304072 | None:intergenic | 40.0% | |
| TTCAATCGTTGTGTCTCTCA+AGG | - | chr5.4:32304270-32304289 | MS.gene53123:CDS | 40.0% | |
| TTCGGATCAATATCTTCACC+AGG | + | chr5.4:32304398-32304417 | None:intergenic | 40.0% | |
| TTGTCGACGAAGCAAATCAA+TGG | + | chr5.4:32304241-32304260 | None:intergenic | 40.0% | |
| ! | ATTGTATGGTGGTCTTGATC+GGG | - | chr5.4:32304518-32304537 | MS.gene53123:CDS | 40.0% |
| ! | CAATGGACTCTTTGTTCTGA+GGG | + | chr5.4:32304224-32304243 | None:intergenic | 40.0% |
| ! | CATTGTATGGTGGTCTTGAT+CGG | - | chr5.4:32304517-32304536 | MS.gene53123:CDS | 40.0% |
| ! | CGCTTATGATTTTGGATACC+TGG | - | chr5.4:32304377-32304396 | MS.gene53123:CDS | 40.0% |
| ! | TCAATGGACTCTTTGTTCTG+AGG | + | chr5.4:32304225-32304244 | None:intergenic | 40.0% |
| ! | TTTGGTGTATGGATAACTCC+AGG | + | chr5.4:32304015-32304034 | None:intergenic | 40.0% |
| !! | ATGACTTGGGTGACTTTTCA+TGG | - | chr5.4:32304354-32304373 | MS.gene53123:CDS | 40.0% |
| !! | ATTGATTTGCTTCGTCGACA+AGG | - | chr5.4:32304240-32304259 | MS.gene53123:CDS | 40.0% |
| !! | TTGATTTGCTTCGTCGACAA+GGG | - | chr5.4:32304241-32304260 | MS.gene53123:CDS | 40.0% |
| AGTAATCAGAGGGTTGAAGG+TGG | + | chr5.4:32304050-32304069 | None:intergenic | 45.0% | |
| ATGGCAGACGTTCCATAAGT+TGG | - | chr5.4:32304611-32304630 | MS.gene53123:CDS | 45.0% | |
| CGCTACTGCAATGCATTGTA+TGG | - | chr5.4:32304504-32304523 | MS.gene53123:CDS | 45.0% | |
| CTGCCATGTCAACAAGCTAT+CGG | + | chr5.4:32304598-32304617 | None:intergenic | 45.0% | |
| GCTGAACTAATGTTGACGTC+TGG | - | chr5.4:32304312-32304331 | MS.gene53123:CDS | 45.0% | |
| GGTTGCAAGTACACTCAATG+TGG | - | chr5.4:32304539-32304558 | MS.gene53123:CDS | 45.0% | |
| GTTGGAAGGTCTCATCAATC+CGG | - | chr5.4:32304570-32304589 | MS.gene53123:CDS | 45.0% | |
| TGTCAACAAGCTATCGGATC+CGG | + | chr5.4:32304592-32304611 | None:intergenic | 45.0% | |
| !! | GATCCGATAGCTTGTTGACA+TGG | - | chr5.4:32304592-32304611 | MS.gene53123:CDS | 45.0% |
| !! | GATGGTCGACTTTTGGTGTA+TGG | + | chr5.4:32304026-32304045 | None:intergenic | 45.0% |
| ! | ATGTGGATCGGGTTGTTGGA+AGG | - | chr5.4:32304556-32304575 | MS.gene53123:CDS | 50.0% |
| ! | CTCAATGTGGATCGGGTTGT+TGG | - | chr5.4:32304552-32304571 | MS.gene53123:CDS | 50.0% |
| CACGCTCGACGTTGAAGTCA+CGG | + | chr5.4:32304200-32304219 | None:intergenic | 55.0% | |
| !! | AGGTGGCGATGGTCGACTTT+TGG | + | chr5.4:32304033-32304052 | None:intergenic | 55.0% |
| CAGAGGGTTGAAGGTGGCGA+TGG | + | chr5.4:32304044-32304063 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 32303859 | 32304707 | 32303859 | ID=MS.gene53123 |
| chr5.4 | mRNA | 32303859 | 32304707 | 32303859 | ID=MS.gene53123.t1;Parent=MS.gene53123 |
| chr5.4 | exon | 32303859 | 32304707 | 32303859 | ID=MS.gene53123.t1.exon1;Parent=MS.gene53123.t1 |
| chr5.4 | CDS | 32303859 | 32304707 | 32303859 | ID=cds.MS.gene53123.t1;Parent=MS.gene53123.t1 |
| Gene Sequence |
| Protein sequence |
