Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53481.t1 | XP_003613224.1 | 98.1 | 108 | 2 | 0 | 61 | 168 | 61 | 168 | 1.50E-42 | 182.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53481.t1 | G7K1C9 | 98.1 | 108 | 2 | 0 | 61 | 168 | 61 | 168 | 1.1e-42 | 182.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene53481 | 0.877826 | 4.60E-69 | -1.69E-46 |
| MS.gene050297 | MS.gene53481 | 0.812224 | 4.72E-51 | -1.69E-46 |
| MS.gene051067 | MS.gene53481 | 0.813638 | 2.31E-51 | -1.69E-46 |
| MS.gene051478 | MS.gene53481 | 0.846976 | 1.55E-59 | -1.69E-46 |
| MS.gene053144 | MS.gene53481 | 0.814081 | 1.85E-51 | -1.69E-46 |
| MS.gene053536 | MS.gene53481 | 0.823104 | 1.66E-53 | -1.69E-46 |
| MS.gene053538 | MS.gene53481 | 0.823365 | 1.44E-53 | -1.69E-46 |
| MS.gene054118 | MS.gene53481 | 0.829242 | 5.74E-55 | -1.69E-46 |
| MS.gene054584 | MS.gene53481 | 0.807751 | 4.34E-50 | -1.69E-46 |
| MS.gene054999 | MS.gene53481 | 0.806467 | 8.11E-50 | -1.69E-46 |
| MS.gene055537 | MS.gene53481 | 0.821051 | 4.96E-53 | -1.69E-46 |
| MS.gene055764 | MS.gene53481 | 0.802319 | 5.94E-49 | -1.69E-46 |
| MS.gene056704 | MS.gene53481 | 0.805736 | 1.16E-49 | -1.69E-46 |
| MS.gene057082 | MS.gene53481 | 0.826093 | 3.28E-54 | -1.69E-46 |
| MS.gene05866 | MS.gene53481 | 0.80656 | 7.75E-50 | -1.69E-46 |
| MS.gene058909 | MS.gene53481 | 0.821979 | 3.03E-53 | -1.69E-46 |
| MS.gene058912 | MS.gene53481 | 0.822121 | 2.81E-53 | -1.69E-46 |
| MS.gene05894 | MS.gene53481 | 0.813642 | 2.31E-51 | -1.69E-46 |
| MS.gene059300 | MS.gene53481 | 0.808629 | 2.82E-50 | -1.69E-46 |
| MS.gene059424 | MS.gene53481 | 0.812547 | 4.01E-51 | -1.69E-46 |
| MS.gene06057 | MS.gene53481 | 0.804177 | 2.45E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53481.t1 | MTR_5g034200 | 99.405 | 168 | 1 | 0 | 1 | 168 | 1 | 168 | 4.74e-122 | 341 |
| MS.gene53481.t1 | MTR_8g064760 | 38.608 | 158 | 73 | 6 | 20 | 167 | 18 | 161 | 5.43e-21 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53481.t1 | AT1G23530 | 33.690 | 187 | 89 | 8 | 11 | 167 | 7 | 188 | 2.93e-16 | 72.8 |
Find 33 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGTTGATATTGAATT+TGG | 0.135097 | 5.3:+23221162 | MS.gene53481:CDS |
| AATTCAATATCAACTTCTTT+TGG | 0.244618 | 5.3:-23221159 | None:intergenic |
| CATTCATCAAATGGATCATT+TGG | 0.283971 | 5.3:+23220988 | MS.gene53481:CDS |
| CTGTTGCTTGTTGTCTTGGA+AGG | 0.386601 | 5.3:+23221052 | MS.gene53481:CDS |
| TCTGCTGTTGCTTGTTGTCT+TGG | 0.387132 | 5.3:+23221048 | MS.gene53481:CDS |
| CCTCCATGTCCATTGTTCAT+AGG | 0.387145 | 5.3:-23221213 | None:intergenic |
| GATGAATGGTGGTCAGGTTC+AGG | 0.392297 | 5.3:-23220973 | None:intergenic |
| TTTCAAAACTCTTCATATCA+TGG | 0.397060 | 5.3:-23221323 | None:intergenic |
| TCATGGTGACCTTGAGCAAC+TGG | 0.407489 | 5.3:-23221306 | None:intergenic |
| TGTTGTTGTTGTTGCTGCAT+TGG | 0.421046 | 5.3:-23220901 | None:intergenic |
| ATTCATCAAATGGATCATTT+GGG | 0.438895 | 5.3:+23220989 | MS.gene53481:CDS |
| AGTATAGGCCAGGTGCATAA+TGG | 0.443427 | 5.3:+23221370 | MS.gene53481:CDS |
| CCTGACCACCATTCATCAAA+TGG | 0.451998 | 5.3:+23220979 | MS.gene53481:CDS |
| CAGGTTCAGGTGGTGCATAC+TGG | 0.460578 | 5.3:-23220960 | None:intergenic |
| CCATTTGATGAATGGTGGTC+AGG | 0.470979 | 5.3:-23220979 | None:intergenic |
| GTGGTTGGATAAACTTGAAC+TGG | 0.498993 | 5.3:-23220928 | None:intergenic |
| AGCAGAAATTACTATTATGA+TGG | 0.518824 | 5.3:-23221031 | None:intergenic |
| GTCCTCCTCAAGGAAAGTAT+AGG | 0.536696 | 5.3:+23221355 | MS.gene53481:CDS |
| CAAATGATCCATTTGATGAA+TGG | 0.542377 | 5.3:-23220987 | None:intergenic |
| TACTGGTATGTGACAGTGGT+TGG | 0.552488 | 5.3:-23220943 | None:intergenic |
| GCTTAACAGGTTTCTGACTG+TGG | 0.553296 | 5.3:-23221104 | None:intergenic |
| ATAAAGCTTGGTCCTCCTCA+AGG | 0.553613 | 5.3:+23221345 | MS.gene53481:CDS |
| TTGTAACTCCATTATGCACC+TGG | 0.558632 | 5.3:-23221378 | None:intergenic |
| CCTCAAGGAAAGTATAGGCC+AGG | 0.560026 | 5.3:+23221360 | MS.gene53481:CDS |
| GGCCTATACTTTCCTTGAGG+AGG | 0.568497 | 5.3:-23221357 | None:intergenic |
| CCTGGCCTATACTTTCCTTG+AGG | 0.589930 | 5.3:-23221360 | None:intergenic |
| GTACAGAAGCCAGTTGCTCA+AGG | 0.601141 | 5.3:+23221297 | MS.gene53481:CDS |
| GAATGGTGGTCAGGTTCAGG+TGG | 0.612964 | 5.3:-23220970 | None:intergenic |
| GCTTCTAAGCCTATGAACAA+TGG | 0.625263 | 5.3:+23221204 | MS.gene53481:CDS |
| AAGCCTATGAACAATGGACA+TGG | 0.633051 | 5.3:+23221210 | MS.gene53481:CDS |
| TGCATACTGGTATGTGACAG+TGG | 0.646713 | 5.3:-23220947 | None:intergenic |
| ATGATCCATTTGATGAATGG+TGG | 0.679103 | 5.3:-23220984 | None:intergenic |
| CCTATGAACAATGGACATGG+AGG | 0.679752 | 5.3:+23221213 | MS.gene53481:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAAGTTGATATTGAATT+TGG | + | chr5.3:23221162-23221181 | MS.gene53481:CDS | 20.0% |
| !!! | AATTCAATATCAACTTCTTT+TGG | - | chr5.3:23221162-23221181 | None:intergenic | 20.0% |
| !!! | TTTTCAAAAAATAATGGACA+TGG | + | chr5.3:23221237-23221256 | MS.gene53481:CDS | 20.0% |
| ! | AGCAGAAATTACTATTATGA+TGG | - | chr5.3:23221034-23221053 | None:intergenic | 25.0% |
| ! | ATTCATCAAATGGATCATTT+GGG | + | chr5.3:23220989-23221008 | MS.gene53481:CDS | 25.0% |
| ! | TTTCAAAACTCTTCATATCA+TGG | - | chr5.3:23221326-23221345 | None:intergenic | 25.0% |
| !!! | AAGAGTTTTGAAATAAAGCT+TGG | + | chr5.3:23221333-23221352 | MS.gene53481:CDS | 25.0% |
| CAAATGATCCATTTGATGAA+TGG | - | chr5.3:23220990-23221009 | None:intergenic | 30.0% | |
| CATTCATCAAATGGATCATT+TGG | + | chr5.3:23220988-23221007 | MS.gene53481:CDS | 30.0% | |
| ! | GGAGGCTTTTCAAAAAATAA+TGG | + | chr5.3:23221231-23221250 | MS.gene53481:CDS | 30.0% |
| !! | TCAAAAAATAATGGACATGG+AGG | + | chr5.3:23221240-23221259 | MS.gene53481:CDS | 30.0% |
| ATGATCCATTTGATGAATGG+TGG | - | chr5.3:23220987-23221006 | None:intergenic | 35.0% | |
| AAGCCTATGAACAATGGACA+TGG | + | chr5.3:23221210-23221229 | MS.gene53481:CDS | 40.0% | |
| GCTTCTAAGCCTATGAACAA+TGG | + | chr5.3:23221204-23221223 | MS.gene53481:CDS | 40.0% | |
| GTGGTTGGATAAACTTGAAC+TGG | - | chr5.3:23220931-23220950 | None:intergenic | 40.0% | |
| ! | TGTTGTTGTTGTTGCTGCAT+TGG | - | chr5.3:23220904-23220923 | None:intergenic | 40.0% |
| !!! | TGCTGCTTTTGATGCTTAAC+AGG | - | chr5.3:23221120-23221139 | None:intergenic | 40.0% |
| ATAAAGCTTGGTCCTCCTCA+AGG | + | chr5.3:23221345-23221364 | MS.gene53481:CDS | 45.0% | |
| CCATTTGATGAATGGTGGTC+AGG | - | chr5.3:23220982-23221001 | None:intergenic | 45.0% | |
| CCTATGAACAATGGACATGG+AGG | + | chr5.3:23221213-23221232 | MS.gene53481:CDS | 45.0% | |
| CCTCCATGTCCATTGTTCAT+AGG | - | chr5.3:23221216-23221235 | None:intergenic | 45.0% | |
| CCTGACCACCATTCATCAAA+TGG | + | chr5.3:23220979-23220998 | MS.gene53481:CDS | 45.0% | |
| GCTTAACAGGTTTCTGACTG+TGG | - | chr5.3:23221107-23221126 | None:intergenic | 45.0% | |
| TACTGGTATGTGACAGTGGT+TGG | - | chr5.3:23220946-23220965 | None:intergenic | 45.0% | |
| TGCATACTGGTATGTGACAG+TGG | - | chr5.3:23220950-23220969 | None:intergenic | 45.0% | |
| ! | CTTCTTTTGGACGTGAGTGT+TGG | - | chr5.3:23221149-23221168 | None:intergenic | 45.0% |
| ! | GTCCTCCTCAAGGAAAGTAT+AGG | + | chr5.3:23221355-23221374 | MS.gene53481:CDS | 45.0% |
| !! | CTGTTGCTTGTTGTCTTGGA+AGG | + | chr5.3:23221052-23221071 | MS.gene53481:CDS | 45.0% |
| !! | TCTGCTGTTGCTTGTTGTCT+TGG | + | chr5.3:23221048-23221067 | MS.gene53481:CDS | 45.0% |
| CCTGGCCTATACTTTCCTTG+AGG | - | chr5.3:23221363-23221382 | None:intergenic | 50.0% | |
| GATGAATGGTGGTCAGGTTC+AGG | - | chr5.3:23220976-23220995 | None:intergenic | 50.0% | |
| GGCCTATACTTTCCTTGAGG+AGG | - | chr5.3:23221360-23221379 | None:intergenic | 50.0% | |
| GTACAGAAGCCAGTTGCTCA+AGG | + | chr5.3:23221297-23221316 | MS.gene53481:CDS | 50.0% | |
| TCATGGTGACCTTGAGCAAC+TGG | - | chr5.3:23221309-23221328 | None:intergenic | 50.0% | |
| ! | CCTCAAGGAAAGTATAGGCC+AGG | + | chr5.3:23221360-23221379 | MS.gene53481:CDS | 50.0% |
| CAGGTTCAGGTGGTGCATAC+TGG | - | chr5.3:23220963-23220982 | None:intergenic | 55.0% | |
| GAATGGTGGTCAGGTTCAGG+TGG | - | chr5.3:23220973-23220992 | None:intergenic | 55.0% | |
| ! | TGTGGCTTTTGCCGTTGCCG+CGG | - | chr5.3:23221089-23221108 | None:intergenic | 60.0% |
| ! | GGAAGGTTTTGCAGCCGCCG+CGG | + | chr5.3:23221069-23221088 | MS.gene53481:CDS | 65.0% |
| ! | TTTTGCAGCCGCCGCGGCAA+CGG | + | chr5.3:23221075-23221094 | MS.gene53481:CDS | 65.0% |
| ! | GGCTTTTGCCGTTGCCGCGG+CGG | - | chr5.3:23221086-23221105 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 23220883 | 23221389 | 23220883 | ID=MS.gene53481 |
| chr5.3 | mRNA | 23220883 | 23221389 | 23220883 | ID=MS.gene53481.t1;Parent=MS.gene53481 |
| chr5.3 | exon | 23220883 | 23221389 | 23220883 | ID=MS.gene53481.t1.exon1;Parent=MS.gene53481.t1 |
| chr5.3 | CDS | 23220883 | 23221389 | 23220883 | ID=cds.MS.gene53481.t1;Parent=MS.gene53481.t1 |
| Gene Sequence |
| Protein sequence |
