Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54356.t1 | XP_013447788.1 | 100 | 98 | 0 | 0 | 1 | 98 | 123 | 220 | 5.60E-45 | 189.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54356.t1 | P19892 | 82.5 | 97 | 16 | 1 | 2 | 98 | 121 | 216 | 7.0e-36 | 151.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54356.t1 | B7FIA6 | 100.0 | 98 | 0 | 0 | 1 | 98 | 123 | 220 | 4.1e-45 | 189.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene54356 | 0.807436 | 5.06E-50 | -1.69E-46 |
| MS.gene04917 | MS.gene54356 | 0.802422 | 5.65E-49 | -1.69E-46 |
| MS.gene04984 | MS.gene54356 | 0.819932 | 8.96E-53 | -1.69E-46 |
| MS.gene050114 | MS.gene54356 | 0.812498 | 4.11E-51 | -1.69E-46 |
| MS.gene050317 | MS.gene54356 | 0.840675 | 7.54E-58 | -1.69E-46 |
| MS.gene050762 | MS.gene54356 | 0.819007 | 1.46E-52 | -1.69E-46 |
| MS.gene050763 | MS.gene54356 | 0.82067 | 6.07E-53 | -1.69E-46 |
| MS.gene051245 | MS.gene54356 | 0.805297 | 1.43E-49 | -1.69E-46 |
| MS.gene052856 | MS.gene54356 | 0.814089 | 1.84E-51 | -1.69E-46 |
| MS.gene053109 | MS.gene54356 | 0.805233 | 1.47E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene54356 | 0.803058 | 4.18E-49 | -1.69E-46 |
| MS.gene055413 | MS.gene54356 | 0.805175 | 1.52E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene54356 | 0.801753 | 7.76E-49 | -1.69E-46 |
| MS.gene057234 | MS.gene54356 | 0.808324 | 3.28E-50 | -1.69E-46 |
| MS.gene057452 | MS.gene54356 | -0.814241 | 1.70E-51 | -1.69E-46 |
| MS.gene058182 | MS.gene54356 | 0.841425 | 4.79E-58 | -1.69E-46 |
| MS.gene058238 | MS.gene54356 | 0.824858 | 6.43E-54 | -1.69E-46 |
| MS.gene059719 | MS.gene54356 | 0.810155 | 1.33E-50 | -1.69E-46 |
| MS.gene060084 | MS.gene54356 | 0.861682 | 8.64E-64 | -1.69E-46 |
| MS.gene060869 | MS.gene54356 | 0.820268 | 7.50E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54356.t1 | MTR_7g405790 | 100.000 | 98 | 0 | 0 | 1 | 98 | 123 | 220 | 1.77e-66 | 199 |
| MS.gene54356.t1 | MTR_7g051940 | 78.351 | 97 | 18 | 1 | 2 | 98 | 122 | 215 | 3.56e-48 | 152 |
| MS.gene54356.t1 | MTR_1g078200 | 49.524 | 105 | 41 | 2 | 2 | 98 | 123 | 223 | 2.52e-25 | 94.7 |
| MS.gene54356.t1 | MTR_8g036220 | 44.340 | 106 | 49 | 2 | 1 | 98 | 124 | 227 | 8.54e-21 | 82.8 |
| MS.gene54356.t1 | MTR_4g064897 | 39.796 | 98 | 56 | 1 | 1 | 98 | 120 | 214 | 7.32e-20 | 80.1 |
| MS.gene54356.t1 | MTR_8g089985 | 49.425 | 87 | 33 | 1 | 1 | 87 | 125 | 200 | 1.30e-19 | 79.7 |
| MS.gene54356.t1 | MTR_1g052475 | 40.385 | 104 | 53 | 3 | 1 | 98 | 123 | 223 | 5.77e-19 | 78.2 |
| MS.gene54356.t1 | MTR_3g086980 | 41.748 | 103 | 51 | 2 | 1 | 98 | 124 | 222 | 2.20e-18 | 76.6 |
| MS.gene54356.t1 | MTR_2g075950 | 57.143 | 63 | 27 | 0 | 1 | 63 | 121 | 183 | 2.91e-17 | 73.6 |
| MS.gene54356.t1 | MTR_2g090865 | 45.545 | 101 | 46 | 3 | 1 | 98 | 119 | 213 | 3.87e-17 | 73.2 |
| MS.gene54356.t1 | MTR_2g005510 | 50.769 | 65 | 32 | 0 | 1 | 65 | 123 | 187 | 5.51e-17 | 72.8 |
| MS.gene54356.t1 | MTR_4g079350 | 42.857 | 98 | 54 | 1 | 1 | 98 | 121 | 216 | 6.16e-17 | 72.8 |
| MS.gene54356.t1 | MTR_2g083860 | 52.308 | 65 | 31 | 0 | 1 | 65 | 121 | 185 | 1.23e-16 | 72.0 |
| MS.gene54356.t1 | MTR_0262s0050 | 50.000 | 66 | 33 | 0 | 1 | 66 | 123 | 188 | 1.41e-16 | 71.6 |
| MS.gene54356.t1 | MTR_3g069510 | 39.796 | 98 | 57 | 1 | 1 | 98 | 120 | 215 | 1.53e-16 | 71.6 |
| MS.gene54356.t1 | MTR_4g088090 | 42.857 | 98 | 54 | 1 | 1 | 98 | 121 | 216 | 1.68e-16 | 71.6 |
| MS.gene54356.t1 | MTR_2g014990 | 42.857 | 98 | 54 | 1 | 1 | 98 | 121 | 216 | 6.07e-16 | 70.1 |
| MS.gene54356.t1 | MTR_2g027660 | 35.000 | 100 | 61 | 2 | 1 | 98 | 114 | 211 | 1.64e-13 | 63.5 |
| MS.gene54356.t1 | MTR_6g079550 | 52.381 | 63 | 30 | 0 | 1 | 63 | 120 | 182 | 9.78e-13 | 61.6 |
| MS.gene54356.t1 | MTR_8g027220 | 50.794 | 63 | 31 | 0 | 1 | 63 | 134 | 196 | 3.60e-12 | 60.1 |
| MS.gene54356.t1 | MTR_6g021790 | 35.000 | 100 | 61 | 2 | 1 | 98 | 114 | 211 | 2.55e-11 | 57.8 |
| MS.gene54356.t1 | MTR_7g081700 | 46.032 | 63 | 34 | 0 | 2 | 64 | 117 | 179 | 9.25e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54356.t1 | AT1G05810 | 82.474 | 97 | 16 | 1 | 2 | 98 | 121 | 216 | 1.82e-50 | 158 |
| MS.gene54356.t1 | AT1G05810 | 82.474 | 97 | 16 | 1 | 2 | 98 | 167 | 262 | 5.68e-50 | 159 |
| MS.gene54356.t1 | AT2G43130 | 78.351 | 97 | 17 | 1 | 2 | 98 | 121 | 213 | 8.03e-48 | 151 |
| MS.gene54356.t1 | AT2G31680 | 77.551 | 98 | 20 | 2 | 2 | 98 | 121 | 217 | 6.58e-47 | 149 |
| MS.gene54356.t1 | AT3G07410 | 75.510 | 98 | 23 | 1 | 1 | 98 | 120 | 216 | 3.69e-45 | 145 |
| MS.gene54356.t1 | AT5G47520 | 50.980 | 102 | 41 | 3 | 2 | 98 | 123 | 220 | 1.74e-25 | 95.1 |
| MS.gene54356.t1 | AT1G07410 | 41.837 | 98 | 53 | 2 | 1 | 98 | 120 | 213 | 1.62e-20 | 82.0 |
| MS.gene54356.t1 | AT4G18430 | 44.444 | 99 | 51 | 2 | 1 | 98 | 121 | 216 | 3.47e-20 | 81.3 |
| MS.gene54356.t1 | AT3G12160 | 53.030 | 66 | 31 | 0 | 1 | 66 | 123 | 188 | 2.95e-19 | 79.0 |
| MS.gene54356.t1 | AT5G46025 | 61.017 | 59 | 23 | 0 | 1 | 59 | 53 | 111 | 4.59e-19 | 75.9 |
| MS.gene54356.t1 | AT5G59150 | 54.098 | 61 | 28 | 0 | 1 | 61 | 69 | 129 | 6.56e-19 | 76.6 |
| MS.gene54356.t1 | AT5G59150 | 54.098 | 61 | 28 | 0 | 1 | 61 | 120 | 180 | 2.51e-18 | 76.3 |
| MS.gene54356.t1 | AT1G09630 | 37.755 | 98 | 59 | 1 | 1 | 98 | 120 | 215 | 2.73e-18 | 76.3 |
| MS.gene54356.t1 | AT5G60860 | 44.898 | 98 | 52 | 1 | 1 | 98 | 121 | 216 | 4.12e-18 | 75.9 |
| MS.gene54356.t1 | AT3G46830 | 54.098 | 61 | 28 | 0 | 1 | 61 | 120 | 180 | 6.37e-18 | 75.1 |
| MS.gene54356.t1 | AT3G46830 | 54.098 | 61 | 28 | 0 | 1 | 61 | 120 | 180 | 6.37e-18 | 75.1 |
| MS.gene54356.t1 | AT1G28550 | 40.816 | 98 | 57 | 1 | 1 | 98 | 121 | 217 | 1.56e-17 | 74.3 |
| MS.gene54356.t1 | AT1G06400 | 47.945 | 73 | 38 | 0 | 1 | 73 | 121 | 193 | 3.95e-17 | 73.2 |
| MS.gene54356.t1 | AT5G45750 | 41.837 | 98 | 54 | 2 | 1 | 98 | 121 | 215 | 4.16e-17 | 73.2 |
| MS.gene54356.t1 | AT2G33870 | 39.796 | 98 | 58 | 1 | 1 | 98 | 121 | 217 | 5.53e-17 | 72.8 |
| MS.gene54356.t1 | AT4G39990 | 50.000 | 66 | 33 | 0 | 1 | 66 | 125 | 190 | 8.88e-17 | 72.4 |
| MS.gene54356.t1 | AT4G18800 | 53.846 | 65 | 30 | 0 | 1 | 65 | 121 | 185 | 1.02e-16 | 72.0 |
| MS.gene54356.t1 | AT5G47960 | 43.564 | 101 | 50 | 3 | 1 | 97 | 123 | 220 | 2.51e-16 | 71.2 |
| MS.gene54356.t1 | AT3G15060 | 53.125 | 64 | 30 | 0 | 1 | 64 | 121 | 184 | 2.76e-16 | 70.9 |
| MS.gene54356.t1 | AT5G65270 | 50.794 | 63 | 31 | 0 | 1 | 63 | 125 | 187 | 6.95e-16 | 70.1 |
| MS.gene54356.t1 | AT1G18200 | 52.381 | 63 | 30 | 0 | 1 | 63 | 121 | 183 | 5.18e-15 | 67.8 |
| MS.gene54356.t1 | AT1G16920 | 42.857 | 98 | 53 | 2 | 1 | 98 | 121 | 215 | 8.13e-15 | 67.0 |
| MS.gene54356.t1 | AT1G73640 | 51.613 | 62 | 30 | 0 | 1 | 62 | 121 | 182 | 8.64e-15 | 67.4 |
| MS.gene54356.t1 | AT4G17170 | 35.000 | 100 | 61 | 2 | 1 | 98 | 114 | 211 | 1.23e-13 | 63.9 |
| MS.gene54356.t1 | AT4G17160 | 37.113 | 97 | 55 | 2 | 2 | 98 | 115 | 205 | 1.43e-13 | 63.5 |
| MS.gene54356.t1 | AT4G35860 | 43.750 | 64 | 36 | 0 | 1 | 64 | 68 | 131 | 6.30e-12 | 58.5 |
| MS.gene54356.t1 | AT4G35860 | 34.653 | 101 | 60 | 3 | 1 | 98 | 114 | 211 | 1.59e-11 | 58.2 |
Find 18 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATGGAAACCTCTGCTT+TGG | 0.343525 | 7.4:-93876322 | MS.gene54356:CDS |
| AAAATAGCCTTTGCTTTGCT+TGG | 0.356179 | 7.4:+93876155 | None:intergenic |
| AGGCTGAATTATCTGTCAAC+AGG | 0.387029 | 7.4:-93876209 | MS.gene54356:CDS |
| GGTGGGAAACAAGTGTGATT+TGG | 0.440037 | 7.4:-93876406 | MS.gene54356:CDS |
| AGATATACAACAATGTTAGC+AGG | 0.522828 | 7.4:-93876257 | MS.gene54356:CDS |
| CCTCAACTCAGACACTTACA+AGG | 0.545261 | 7.4:-93876229 | MS.gene54356:CDS |
| AAAAGTCTTGCGGAATCAGA+AGG | 0.554276 | 7.4:-93876351 | MS.gene54356:CDS |
| TACGACGGTGGCAATGATGC+TGG | 0.577423 | 7.4:-93876427 | None:intergenic |
| AGAGATGTGAGCATTGAAGA+AGG | 0.582505 | 7.4:-93876375 | MS.gene54356:CDS |
| CCTTGTAAGTGTCTGAGTTG+AGG | 0.585872 | 7.4:+93876229 | None:intergenic |
| ATTTGTAGAGTCCAAAGCAG+AGG | 0.603895 | 7.4:+93876311 | None:intergenic |
| TCTACATCCAAGCAAAGCAA+AGG | 0.634847 | 7.4:-93876162 | MS.gene54356:CDS |
| TGAAGAAGGCAAAAGTCTTG+CGG | 0.635809 | 7.4:-93876361 | MS.gene54356:CDS |
| GGCTGAATTATCTGTCAACA+GGG | 0.647451 | 7.4:-93876208 | MS.gene54356:CDS |
| AGGGTCAGTCTTGTTAACAA+TGG | 0.671243 | 7.4:-93876189 | MS.gene54356:CDS |
| ATACAACAATGTTAGCAGGA+AGG | 0.674114 | 7.4:-93876253 | MS.gene54356:CDS |
| GACGGTGGCAATGATGCTGG+TGG | 0.677201 | 7.4:-93876424 | None:intergenic |
| ACGGTGGCAATGATGCTGGT+GGG | 0.711154 | 7.4:-93876423 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATATACAACAATGTTAGC+AGG | - | chr7.4:93876297-93876316 | MS.gene54356:CDS | 30.0% | |
| !! | ATCAGAAGGTTTGTTTTTCA+TGG | - | chr7.4:93876217-93876236 | MS.gene54356:CDS | 30.0% |
| AAAATAGCCTTTGCTTTGCT+TGG | + | chr7.4:93876402-93876421 | None:intergenic | 35.0% | |
| ATACAACAATGTTAGCAGGA+AGG | - | chr7.4:93876301-93876320 | MS.gene54356:CDS | 35.0% | |
| AAAAGTCTTGCGGAATCAGA+AGG | - | chr7.4:93876203-93876222 | MS.gene54356:CDS | 40.0% | |
| AGGCTGAATTATCTGTCAAC+AGG | - | chr7.4:93876345-93876364 | MS.gene54356:CDS | 40.0% | |
| ATTTGTAGAGTCCAAAGCAG+AGG | + | chr7.4:93876246-93876265 | None:intergenic | 40.0% | |
| GGCTGAATTATCTGTCAACA+GGG | - | chr7.4:93876346-93876365 | MS.gene54356:CDS | 40.0% | |
| TCTACATCCAAGCAAAGCAA+AGG | - | chr7.4:93876392-93876411 | MS.gene54356:CDS | 40.0% | |
| TGAAGAAGGCAAAAGTCTTG+CGG | - | chr7.4:93876193-93876212 | MS.gene54356:CDS | 40.0% | |
| TTTCATGGAAACCTCTGCTT+TGG | - | chr7.4:93876232-93876251 | MS.gene54356:CDS | 40.0% | |
| !! | AGAGATGTGAGCATTGAAGA+AGG | - | chr7.4:93876179-93876198 | MS.gene54356:CDS | 40.0% |
| !! | AGGGTCAGTCTTGTTAACAA+TGG | - | chr7.4:93876365-93876384 | MS.gene54356:CDS | 40.0% |
| !! | TGTGAAGACAGCTTTTGAGA+TGG | - | chr7.4:93876265-93876284 | MS.gene54356:CDS | 40.0% |
| CCTCAACTCAGACACTTACA+AGG | - | chr7.4:93876325-93876344 | MS.gene54356:CDS | 45.0% | |
| CCTTGTAAGTGTCTGAGTTG+AGG | + | chr7.4:93876328-93876347 | None:intergenic | 45.0% | |
| GGTGGGAAACAAGTGTGATT+TGG | - | chr7.4:93876148-93876167 | MS.gene54356:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 93876140 | 93876436 | 93876140 | ID=MS.gene54356 |
| chr7.4 | mRNA | 93876140 | 93876436 | 93876140 | ID=MS.gene54356.t1;Parent=MS.gene54356 |
| chr7.4 | exon | 93876140 | 93876436 | 93876140 | ID=MS.gene54356.t1.exon1;Parent=MS.gene54356.t1 |
| chr7.4 | CDS | 93876140 | 93876436 | 93876140 | ID=cds.MS.gene54356.t1;Parent=MS.gene54356.t1 |
| Gene Sequence |
| Protein sequence |
