AlfalfaGEDB | MS.gene55324

M. sativa cultivar XinJiangDaYe / MS.gene55324

KEGG: K12581
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04857.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55324.t1	XP_024639118.1	91.1	282	25	0	1	282	1	282	4.10E-149	537.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55324.t1	Q9FMS6	56.6	274	113	3	10	280	7	277	9.8e-83	308.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55324.t1	A0A396HQ65	91.1	282	25	0	1	282	1	282	3.0e-149	537.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene55324	MS.gene007154	PPI
MS.gene55324	MS.gene72977	PPI
MS.gene55324	MS.gene025628	PPI
MS.gene034250	MS.gene55324	PPI
MS.gene034816	MS.gene55324	PPI
MS.gene72978	MS.gene55324	PPI
MS.gene034815	MS.gene55324	PPI
MS.gene55324	MS.gene065709	PPI
MS.gene55324	MS.gene065707	PPI
MS.gene55324	MS.gene000609	PPI
MS.gene025629	MS.gene55324	PPI
MS.gene41100	MS.gene55324	PPI
MS.gene55324	MS.gene21541	PPI
MS.gene55324	MS.gene41097	PPI
MS.gene55324	MS.gene41101	PPI
MS.gene065708	MS.gene55324	PPI
MS.gene053225	MS.gene55324	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55324.t1	AT5G22250	56.569	274	113	3	10	280	7	277	1.75e-105	308
MS.gene55324.t1	AT3G44260	55.390	269	111	5	15	280	17	279	2.39e-98	290
MS.gene55324.t1	AT5G10960	45.818	275	140	6	7	280	1	267	4.35e-79	241
MS.gene55324.t1	AT2G32070	47.940	267	130	6	15	280	10	268	1.38e-78	239
MS.gene55324.t1	AT1G80780	45.865	266	136	5	15	280	10	267	2.29e-74	229
MS.gene55324.t1	AT1G80780	45.865	266	136	5	15	280	10	267	2.29e-74	229
MS.gene55324.t1	AT1G80780	45.865	266	136	5	15	280	10	267	4.05e-74	228
MS.gene55324.t1	AT1G15920	45.324	278	142	6	9	282	8	279	5.22e-71	221
MS.gene55324.t1	AT1G15920	45.324	278	142	6	9	282	8	279	5.22e-71	221
MS.gene55324.t1	AT1G61470	39.024	246	125	8	19	259	4	229	1.78e-42	147
MS.gene55324.t1	AT1G06450	33.824	272	157	10	10	278	3	254	5.48e-38	137
MS.gene55324.t1	AT1G27820	36.260	262	140	9	13	267	2	243	3.94e-36	131
MS.gene55324.t1	AT1G27890	37.255	255	135	9	19	267	3	238	7.15e-35	127
MS.gene55324.t1	AT3G44240	35.745	235	126	9	31	260	3	217	9.25e-33	120

Find 58 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCATGGCGCTTATGATTT+TGG	0.117389	5.2:-32847915	MS.gene55324:CDS
AAATACGCTGGAGTAGTATT+TGG	0.224745	5.2:-32847618	MS.gene55324:CDS
TGAATATGGTGAATATTCTT+TGG	0.278766	5.2:-32848315	MS.gene55324:CDS
CGTGATGTTCTTCCTGAATA+TGG	0.278966	5.2:-32848329	MS.gene55324:CDS
CAATCATTGTCCGAGAAGTT+TGG	0.285300	5.2:-32848385	MS.gene55324:CDS
TTGCCAAATGTAGCGATTAT+TGG	0.305326	5.2:+32848118	None:intergenic
CTTGGACTCACTTTAATAGA+TGG	0.314324	5.2:-32848167	MS.gene55324:CDS
CAAACTTCTCGGACAATGAT+TGG	0.324520	5.2:+32848386	None:intergenic
AACATTCTGGAAATGTTATT+CGG	0.326580	5.2:-32847834	MS.gene55324:CDS
AAGAAATCTCCCTGATAGTT+TGG	0.329665	5.2:-32847868	MS.gene55324:CDS
GCTGAACTAATGTTGACTTC+TGG	0.360897	5.2:-32847972	MS.gene55324:CDS
AATCATTGTCCGAGAAGTTT+GGG	0.374317	5.2:-32848384	MS.gene55324:CDS
ACACCAATAATCGCTACATT+TGG	0.390799	5.2:-32848121	MS.gene55324:CDS
AGTTTAAGATCATCAACATT+TGG	0.398324	5.2:+32848197	None:intergenic
CGATTATTGGTGTCAAAATC+AGG	0.404880	5.2:+32848131	None:intergenic
AAAGAATATTCACCATATTC+AGG	0.416250	5.2:+32848317	None:intergenic
AGGTGGCGATGGTCGACTTT+TGG	0.420934	5.2:+32848254	None:intergenic
TCGATAGACACTGAATTTCC+TGG	0.427087	5.2:-32848290	MS.gene55324:CDS
CATTGTATGGTGGTCTTGAT+CGG	0.438293	5.2:-32847767	MS.gene55324:CDS
TGTCAACAAGCTGTCGGATC+CGG	0.457649	5.2:+32847695	None:intergenic
AACTATCAGGGAGATTTCTT+CGG	0.461082	5.2:+32847871	None:intergenic
AGTTGTGTGCCCAAACTTCT+CGG	0.466420	5.2:+32848375	None:intergenic
TATTCAAGAAATCAATGACT+TGG	0.469568	5.2:-32847944	MS.gene55324:CDS
CTCAACGTGGATCGGGCTGT+TGG	0.476111	5.2:-32847732	MS.gene55324:CDS
CGCTACTGCAATGCATTGTA+TGG	0.477422	5.2:-32847780	MS.gene55324:CDS
ATGGCAGACGTTTCATAAGT+TGG	0.479969	5.2:-32847673	MS.gene55324:CDS
TGTAGTAATCAGAGGGTTGA+AGG	0.480186	5.2:+32848234	None:intergenic
CAAGTACACTCAACGTGGAT+CGG	0.507101	5.2:-32847740	MS.gene55324:CDS
AAGTACACTCAACGTGGATC+GGG	0.508841	5.2:-32847739	MS.gene55324:CDS
TTCTGGAAATGTTATTCGGA+AGG	0.512713	5.2:-32847830	MS.gene55324:CDS
ATTGTATGGTGGTCTTGATC+GGG	0.514058	5.2:-32847766	MS.gene55324:CDS
ACGTGGATCGGGCTGTTGGA+AGG	0.522206	5.2:-32847728	MS.gene55324:CDS
TTGTTGACGAAGCAAATCAA+TGG	0.531925	5.2:+32848046	None:intergenic
GTTGGAAGGTCTCATCAATC+CGG	0.537925	5.2:-32847714	MS.gene55324:CDS
TTCAATCGTTGTGTCTCTCA+AGG	0.540052	5.2:-32848014	MS.gene55324:CDS
ATTCAAGAAATCAATGACTT+GGG	0.542864	5.2:-32847943	MS.gene55324:CDS
GATCTTAAACTCATCCAACT+TGG	0.545194	5.2:-32848185	MS.gene55324:CDS
CAATGGAATCCTTGTTGTGA+GGG	0.555858	5.2:+32848063	None:intergenic
CTATTAAAGTGAGTCCAAGT+TGG	0.558411	5.2:+32848171	None:intergenic
TTTGGTGTATGGATAACTCC+AGG	0.559457	5.2:+32848272	None:intergenic
CAGAGGGTTGAAGGTGGCGA+TGG	0.559485	5.2:+32848243	None:intergenic
TCAACATTAGTTCAGCAAAA+CGG	0.564358	5.2:+32847979	None:intergenic
GAAGAACATCACGAATGAGA+TGG	0.565749	5.2:+32848339	None:intergenic
ATTGATTTGCTTCGTCAACA+AGG	0.572268	5.2:-32848044	MS.gene55324:CDS
GATCCGACAGCTTGTTGACA+TGG	0.577163	5.2:-32847692	MS.gene55324:CDS
AAAACTCTTCCAAACTATCA+GGG	0.580045	5.2:+32847859	None:intergenic
CATACAATGCATTGCAGTAG+CGG	0.583860	5.2:+32847781	None:intergenic
GACGTATCTGTAGTAATCAG+AGG	0.593946	5.2:+32848226	None:intergenic
TTGATTTGCTTCGTCAACAA+GGG	0.596345	5.2:-32848043	MS.gene55324:CDS
TACTGCAATGCATTGTATGG+TGG	0.597106	5.2:-32847777	MS.gene55324:CDS
ACGTATCTGTAGTAATCAGA+GGG	0.599199	5.2:+32848227	None:intergenic
CTGCCATGTCAACAAGCTGT+CGG	0.613501	5.2:+32847689	None:intergenic
CTCACTTTAATAGATGGTTG+CGG	0.615126	5.2:-32848161	MS.gene55324:CDS
TCAATGGAATCCTTGTTGTG+AGG	0.615657	5.2:+32848062	None:intergenic
CGAGCGTGACCCTCACAACA+AGG	0.631507	5.2:-32848072	MS.gene55324:CDS
TATTGGTGTCAAAATCAGGA+AGG	0.658615	5.2:+32848135	None:intergenic
AGTAATCAGAGGGTTGAAGG+TGG	0.690515	5.2:+32848237	None:intergenic
GGTTGCAAGTACACTCAACG+TGG	0.728911	5.2:-32847745	MS.gene55324:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAGAATATTCACCATATTC+AGG	+	chr5.2:32847710-32847729	None:intergenic	25.0%
!	ATTCAAGAAATCAATGACTT+GGG	-	chr5.2:32848081-32848100	MS.gene55324:CDS	25.0%
!	TATTCAAGAAATCAATGACT+TGG	-	chr5.2:32848080-32848099	MS.gene55324:CDS	25.0%
!	TGAATATGGTGAATATTCTT+TGG	-	chr5.2:32847709-32847728	MS.gene55324:CDS	25.0%
!!	AACATTCTGGAAATGTTATT+CGG	-	chr5.2:32848190-32848209	MS.gene55324:CDS	25.0%
!!	AGTTTAAGATCATCAACATT+TGG	+	chr5.2:32847830-32847849	None:intergenic	25.0%
	AAAAACTCTTCCAAACTATC+AGG	+	chr5.2:32848169-32848188	None:intergenic	30.0%
	AAAACTCTTCCAAACTATCA+GGG	+	chr5.2:32848168-32848187	None:intergenic	30.0%
	AGAGAATTTGAAAAATACGC+TGG	-	chr5.2:32848394-32848413	MS.gene55324:CDS	30.0%
	TCAACATTAGTTCAGCAAAA+CGG	+	chr5.2:32848048-32848067	None:intergenic	30.0%
	AAATACGCTGGAGTAGTATT+TGG	-	chr5.2:32848406-32848425	MS.gene55324:CDS	35.0%
	AACTATCAGGGAGATTTCTT+CGG	+	chr5.2:32848156-32848175	None:intergenic	35.0%
	AAGAAATCTCCCTGATAGTT+TGG	-	chr5.2:32848156-32848175	MS.gene55324:CDS	35.0%
	AATCATTGTCCGAGAAGTTT+GGG	-	chr5.2:32847640-32847659	MS.gene55324:CDS	35.0%
	ACACCAATAATCGCTACATT+TGG	-	chr5.2:32847903-32847922	MS.gene55324:CDS	35.0%
	ACGTATCTGTAGTAATCAGA+GGG	+	chr5.2:32847800-32847819	None:intergenic	35.0%
	CTATTAAAGTGAGTCCAAGT+TGG	+	chr5.2:32847856-32847875	None:intergenic	35.0%
	CTCACTTTAATAGATGGTTG+CGG	-	chr5.2:32847863-32847882	MS.gene55324:CDS	35.0%
	CTTGGACTCACTTTAATAGA+TGG	-	chr5.2:32847857-32847876	MS.gene55324:CDS	35.0%
	GATCTTAAACTCATCCAACT+TGG	-	chr5.2:32847839-32847858	MS.gene55324:CDS	35.0%
	TTGCCAAATGTAGCGATTAT+TGG	+	chr5.2:32847909-32847928	None:intergenic	35.0%
	TTGTTGACGAAGCAAATCAA+TGG	+	chr5.2:32847981-32848000	None:intergenic	35.0%
!	CGCTTATGATTTTGGATACT+TGG	-	chr5.2:32848117-32848136	MS.gene55324:CDS	35.0%
!	GGAAGAGTTTTTGAACATTC+TGG	-	chr5.2:32848177-32848196	MS.gene55324:CDS	35.0%
!	TTCTGGAAATGTTATTCGGA+AGG	-	chr5.2:32848194-32848213	MS.gene55324:CDS	35.0%
!	TTTCATGGCGCTTATGATTT+TGG	-	chr5.2:32848109-32848128	MS.gene55324:CDS	35.0%
!!	ATTGATTTGCTTCGTCAACA+AGG	-	chr5.2:32847980-32847999	MS.gene55324:CDS	35.0%
!!	CGATTATTGGTGTCAAAATC+AGG	+	chr5.2:32847896-32847915	None:intergenic	35.0%
!!	TATTGGTGTCAAAATCAGGA+AGG	+	chr5.2:32847892-32847911	None:intergenic	35.0%
!!	TTGATTTGCTTCGTCAACAA+GGG	-	chr5.2:32847981-32848000	MS.gene55324:CDS	35.0%
	ATGGCAGACGTTTCATAAGT+TGG	-	chr5.2:32848351-32848370	MS.gene55324:CDS	40.0%
	CAAACTTCTCGGACAATGAT+TGG	+	chr5.2:32847641-32847660	None:intergenic	40.0%
	CAATCATTGTCCGAGAAGTT+TGG	-	chr5.2:32847639-32847658	MS.gene55324:CDS	40.0%
	CATACAATGCATTGCAGTAG+CGG	+	chr5.2:32848246-32848265	None:intergenic	40.0%
	CGTGATGTTCTTCCTGAATA+TGG	-	chr5.2:32847695-32847714	MS.gene55324:CDS	40.0%
	GAAGAACATCACGAATGAGA+TGG	+	chr5.2:32847688-32847707	None:intergenic	40.0%
	GACGTATCTGTAGTAATCAG+AGG	+	chr5.2:32847801-32847820	None:intergenic	40.0%
	TACTGCAATGCATTGTATGG+TGG	-	chr5.2:32848247-32848266	MS.gene55324:CDS	40.0%
	TCGATAGACACTGAATTTCC+TGG	-	chr5.2:32847734-32847753	MS.gene55324:CDS	40.0%
	TGTAGTAATCAGAGGGTTGA+AGG	+	chr5.2:32847793-32847812	None:intergenic	40.0%
	TTCAATCGTTGTGTCTCTCA+AGG	-	chr5.2:32848010-32848029	MS.gene55324:CDS	40.0%
!	ATTGTATGGTGGTCTTGATC+GGG	-	chr5.2:32848258-32848277	MS.gene55324:CDS	40.0%
!	CAATGGAATCCTTGTTGTGA+GGG	+	chr5.2:32847964-32847983	None:intergenic	40.0%
!	CATTGTATGGTGGTCTTGAT+CGG	-	chr5.2:32848257-32848276	MS.gene55324:CDS	40.0%
!	TCAATGGAATCCTTGTTGTG+AGG	+	chr5.2:32847965-32847984	None:intergenic	40.0%
!	TTTGGTGTATGGATAACTCC+AGG	+	chr5.2:32847755-32847774	None:intergenic	40.0%
!!	ATGACTTGGGTGACTTTTCA+TGG	-	chr5.2:32848094-32848113	MS.gene55324:CDS	40.0%
!!	GCTGAACTAATGTTGACTTC+TGG	-	chr5.2:32848052-32848071	MS.gene55324:CDS	40.0%
	AAGTACACTCAACGTGGATC+GGG	-	chr5.2:32848285-32848304	MS.gene55324:CDS	45.0%
	AGTAATCAGAGGGTTGAAGG+TGG	+	chr5.2:32847790-32847809	None:intergenic	45.0%
	AGTTGTGTGCCCAAACTTCT+CGG	+	chr5.2:32847652-32847671	None:intergenic	45.0%
	CAAGTACACTCAACGTGGAT+CGG	-	chr5.2:32848284-32848303	MS.gene55324:CDS	45.0%
	CGCTACTGCAATGCATTGTA+TGG	-	chr5.2:32848244-32848263	MS.gene55324:CDS	45.0%
	GTTGGAAGGTCTCATCAATC+CGG	-	chr5.2:32848310-32848329	MS.gene55324:CDS	45.0%
!!	GATGGTCGACTTTTGGTGTA+TGG	+	chr5.2:32847766-32847785	None:intergenic	45.0%
	CTGCCATGTCAACAAGCTGT+CGG	+	chr5.2:32848338-32848357	None:intergenic	50.0%
	GGTTGCAAGTACACTCAACG+TGG	-	chr5.2:32848279-32848298	MS.gene55324:CDS	50.0%
	TGTCAACAAGCTGTCGGATC+CGG	+	chr5.2:32848332-32848351	None:intergenic	50.0%
!!	GATCCGACAGCTTGTTGACA+TGG	-	chr5.2:32848332-32848351	MS.gene55324:CDS	50.0%
!!	AGGTGGCGATGGTCGACTTT+TGG	+	chr5.2:32847773-32847792	None:intergenic	55.0%
	ACGTGGATCGGGCTGTTGGA+AGG	-	chr5.2:32848296-32848315	MS.gene55324:CDS	60.0%
	CAGAGGGTTGAAGGTGGCGA+TGG	+	chr5.2:32847784-32847803	None:intergenic	60.0%
	CGAGCGTGACCCTCACAACA+AGG	-	chr5.2:32847952-32847971	MS.gene55324:CDS	60.0%
	CTCAACGTGGATCGGGCTGT+TGG	-	chr5.2:32848292-32848311	MS.gene55324:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	32847599	32848447	32847599	ID=MS.gene55324
chr5.2	mRNA	32847599	32848447	32847599	ID=MS.gene55324.t1;Parent=MS.gene55324
chr5.2	exon	32847599	32848447	32847599	ID=MS.gene55324.t1.exon1;Parent=MS.gene55324.t1
chr5.2	CDS	32847599	32848447	32847599	ID=cds.MS.gene55324.t1;Parent=MS.gene55324.t1

Gene Sequence

>MS.gene55324

ATGACAAAAACGTGCATGCTGAGTGAAGAGTTATCAAAACCAATCATTGTCCGAGAAGTTTGGGCACACAACTTAGAGAATGAATTCCATCTCATTCGTGATGTTCTTCCTGAATATGGTGAATATTCTTTGGTCTCGATAGACACTGAATTTCCTGGAGTTATCCATACACCAAAAGTCGACCATCGCCACCTTCAACCCTCTGATTACTACAGATACGTCTTAAAGCCAAATGTTGATGATCTTAAACTCATCCAACTTGGACTCACTTTAATAGATGGTTGCGGACACCTTCCTGATTTTGACACCAATAATCGCTACATTTGGCAATTCAACTTCTGTGACTTCAACGTCGAGCGTGACCCTCACAACAAGGATTCCATTGATTTGCTTCGTCAACAAGGGATTGACTTCAATCGTTGTGTCTCTCAAGGTGTGAATTCATTCCGTTTTGCTGAACTAATGTTGACTTCTGGACTTCTATTCAAGAAATCAATGACTTGGGTGACTTTTCATGGCGCTTATGATTTTGGATACTTGGTGAAGATATTGATCCGAAGAAATCTCCCTGATAGTTTGGAAGAGTTTTTGAACATTCTGGAAATGTTATTCGGAAGGAATGTGTATGATTTGAAACATATGATCCGCTACTGCAATGCATTGTATGGTGGTCTTGATCGGGTTGCAAGTACACTCAACGTGGATCGGGCTGTTGGAAGGTCTCATCAATCCGGATCCGACAGCTTGTTGACATGGCAGACGTTTCATAAGTTGGTGCAAACACATCTCAGTCACAGAGAATTTGAAAAATACGCTGGAGTAGTATTTGGATTAGAAGTTTGTCGATAA

Protein sequence

>MS.gene55324.t1

MTKTCMLSEELSKPIIVREVWAHNLENEFHLIRDVLPEYGEYSLVSIDTEFPGVIHTPKVDHRHLQPSDYYRYVLKPNVDDLKLIQLGLTLIDGCGHLPDFDTNNRYIWQFNFCDFNVERDPHNKDSIDLLRQQGIDFNRCVSQGVNSFRFAELMLTSGLLFKKSMTWVTFHGAYDFGYLVKILIRRNLPDSLEEFLNILEMLFGRNVYDLKHMIRYCNALYGGLDRVASTLNVDRAVGRSHQSGSDSLLTWQTFHKLVQTHLSHREFEKYAGVVFGLEVCR