AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55767


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55767.t1 MTR_8g099150 100.000 106 0 0 1 106 1 106 2.78e-76 220
MS.gene55767.t1 MTR_8g016300 98.113 106 2 0 1 106 1 106 4.01e-75 217
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55767.t1 AT5G55140 63.810 105 37 1 3 106 1 105 1.15e-45 143

Find 42 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGCGCCTATGTTGCTTTC+TGG 0.240202 4.3:-4980730 None:intergenic
AACGCTAGTGAGGGGTATTC+CGG 0.301510 4.3:+4980704 MS.gene55767:CDS
GGTTTCCTCCTTCTCTTTGC+GGG 0.349900 4.3:-4980964 None:intergenic
TCAACCTGCTTCTTCTGCTT+AGG 0.384301 4.3:+4981022 MS.gene55767:CDS
CAACCTGCTTCTTCTGCTTA+GGG 0.390606 4.3:+4981023 MS.gene55767:CDS
GATGGAACACACCTACTGTT+AGG 0.400025 4.3:+4980796 MS.gene55767:CDS
CACTAGCGTTATGTAGAGAT+TGG 0.401453 4.3:-4980691 None:intergenic
ACGCTAGTGAGGGGTATTCC+GGG 0.415196 4.3:+4980705 MS.gene55767:CDS
GGGTTTCCTCCTTCTCTTTG+CGG 0.418532 4.3:-4980965 None:intergenic
AGATTGGGGCTATAAGCAAT+TGG 0.427253 4.3:-4980675 None:intergenic
CATGAGCATGAATGCGTTCA+AGG 0.431873 4.3:+4980635 None:intergenic
AAGCAGAAGAAGCAGGTTGA+TGG 0.435151 4.3:-4981019 None:intergenic
ACTAGCGTTATGTAGAGATT+GGG 0.460548 4.3:-4980690 None:intergenic
ATGGAACACACCTACTGTTA+GGG 0.464145 4.3:+4980797 MS.gene55767:CDS
GTTAGTTGTTGTTGAGACAG+AGG 0.465463 4.3:+4980929 MS.gene55767:CDS
CTATGTTGCTTTCTGGTCCC+CGG 0.474886 4.3:-4980723 None:intergenic
CAGAGCTCTTCGTCCTCCAT+TGG 0.509479 4.3:+4980989 MS.gene55767:CDS
TGTTCCATCGCATGACAGTT+CGG 0.511917 4.3:-4980782 None:intergenic
AAAGCAACATAGGCGCACTT+TGG 0.520220 4.3:+4980734 MS.gene55767:CDS
GGTTGATGGTTTATGACCAA+TGG 0.522943 4.3:-4981005 None:intergenic
GATTGGGGCTATAAGCAATT+GGG 0.527244 4.3:-4980674 None:intergenic
TGTATTTGATATAGGTCAAG+AGG 0.544788 4.3:+4980907 MS.gene55767:intron
GTACCCTAAGCAGAAGAAGC+AGG 0.555500 4.3:-4981026 None:intergenic
GTACAAGGCCCGCAAAGAGA+AGG 0.555978 4.3:+4980956 MS.gene55767:CDS
CGGGGACCAGAAAGCAACAT+AGG 0.565331 4.3:+4980724 MS.gene55767:CDS
TGGAGGACGAAGAGCTCTGT+GGG 0.570519 4.3:-4980985 None:intergenic
TGTTAGGGGAATGATCCAGC+AGG 0.585091 4.3:+4980812 MS.gene55767:CDS
GCTATAAGCAATTGGGACAC+AGG 0.590348 4.3:-4980667 None:intergenic
GCAACCGAACTGTCATGCGA+TGG 0.602415 4.3:+4980778 MS.gene55767:CDS
CAAGGCCCGCAAAGAGAAGG+AGG 0.604236 4.3:+4980959 MS.gene55767:CDS
ATCTCTACATAACGCTAGTG+AGG 0.609634 4.3:+4980694 MS.gene55767:CDS
CTAGCGTTATGTAGAGATTG+GGG 0.616077 4.3:-4980689 None:intergenic
TGGATCATTCCCCTAACAGT+AGG 0.620579 4.3:-4980807 None:intergenic
TGATGGTTTATGACCAATGG+AGG 0.622285 4.3:-4981002 None:intergenic
CAGTTCGGTTGCATTTGCCG+AGG 0.626107 4.3:-4980767 None:intergenic
TCTCTACATAACGCTAGTGA+GGG 0.627577 4.3:+4980695 MS.gene55767:CDS
TGAGACAGAGGAGATGTACA+AGG 0.642473 4.3:+4980941 MS.gene55767:CDS
ACTTTGGAAGCACTACGCCT+CGG 0.645701 4.3:+4980750 MS.gene55767:CDS
TGGAACACACCTACTGTTAG+GGG 0.654422 4.3:+4980798 MS.gene55767:CDS
ATGGAGGACGAAGAGCTCTG+TGG 0.663844 4.3:-4980986 None:intergenic
CGCTAGTGAGGGGTATTCCG+GGG 0.667158 4.3:+4980706 MS.gene55767:CDS
CTCTACATAACGCTAGTGAG+GGG 0.670443 4.3:+4980696 MS.gene55767:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GGTAACTTTGTATTTGATAT+AGG + chr4.3:4980899-4980918 MS.gene55767:intron 25.0%
ACCAATGTTGTTAGTATACA+GGG - chr4.3:4980882-4980901 None:intergenic 30.0%
TACCAATGTTGTTAGTATAC+AGG - chr4.3:4980883-4980902 None:intergenic 30.0%
TGTATTTGATATAGGTCAAG+AGG + chr4.3:4980907-4980926 MS.gene55767:intron 30.0%
ACTAGCGTTATGTAGAGATT+GGG - chr4.3:4980693-4980712 None:intergenic 35.0%
GGAGATAGGTAGTTTCAAAA+TGG - chr4.3:4980861-4980880 None:intergenic 35.0%
TCCCTGTATACTAACAACAT+TGG + chr4.3:4980878-4980897 MS.gene55767:intron 35.0%
TTGTTAGTATACAGGGAGAT+AGG - chr4.3:4980875-4980894 None:intergenic 35.0%
AGATTGGGGCTATAAGCAAT+TGG - chr4.3:4980678-4980697 None:intergenic 40.0%
ATCTCTACATAACGCTAGTG+AGG + chr4.3:4980694-4980713 MS.gene55767:CDS 40.0%
ATGGAACACACCTACTGTTA+GGG + chr4.3:4980797-4980816 MS.gene55767:CDS 40.0%
CACTAGCGTTATGTAGAGAT+TGG - chr4.3:4980694-4980713 None:intergenic 40.0%
CTAGCGTTATGTAGAGATTG+GGG - chr4.3:4980692-4980711 None:intergenic 40.0%
GATTGGGGCTATAAGCAATT+GGG - chr4.3:4980677-4980696 None:intergenic 40.0%
TCTCTACATAACGCTAGTGA+GGG + chr4.3:4980695-4980714 MS.gene55767:CDS 40.0%
TGATGGTTTATGACCAATGG+AGG - chr4.3:4981005-4981024 None:intergenic 40.0%
! GTTAGTTGTTGTTGAGACAG+AGG + chr4.3:4980929-4980948 MS.gene55767:CDS 40.0%
!! GGTTGATGGTTTATGACCAA+TGG - chr4.3:4981008-4981027 None:intergenic 40.0%
AAAGCAACATAGGCGCACTT+TGG + chr4.3:4980734-4980753 MS.gene55767:CDS 45.0%
CTCTACATAACGCTAGTGAG+GGG + chr4.3:4980696-4980715 MS.gene55767:CDS 45.0%
GATGGAACACACCTACTGTT+AGG + chr4.3:4980796-4980815 MS.gene55767:CDS 45.0%
GCTATAAGCAATTGGGACAC+AGG - chr4.3:4980670-4980689 None:intergenic 45.0%
TGAGACAGAGGAGATGTACA+AGG + chr4.3:4980941-4980960 MS.gene55767:CDS 45.0%
TGGAACACACCTACTGTTAG+GGG + chr4.3:4980798-4980817 MS.gene55767:CDS 45.0%
TGGATCATTCCCCTAACAGT+AGG - chr4.3:4980810-4980829 None:intergenic 45.0%
TGTTCCATCGCATGACAGTT+CGG - chr4.3:4980785-4980804 None:intergenic 45.0%
! AAGCAGAAGAAGCAGGTTGA+TGG - chr4.3:4981022-4981041 None:intergenic 45.0%
GATCTTGTGCTGAAACCTGC+TGG - chr4.3:4980830-4980849 None:intergenic 50.0%
GGGTTTCCTCCTTCTCTTTG+CGG - chr4.3:4980968-4980987 None:intergenic 50.0%
GGTTTCCTCCTTCTCTTTGC+GGG - chr4.3:4980967-4980986 None:intergenic 50.0%
TGTTAGGGGAATGATCCAGC+AGG + chr4.3:4980812-4980831 MS.gene55767:CDS 50.0%
!! AACGCTAGTGAGGGGTATTC+CGG + chr4.3:4980704-4980723 MS.gene55767:CDS 50.0%
!! ACTTTGGAAGCACTACGCCT+CGG + chr4.3:4980750-4980769 MS.gene55767:CDS 50.0%
!! AGTGCGCCTATGTTGCTTTC+TGG - chr4.3:4980733-4980752 None:intergenic 50.0%
!! CTATGTTGCTTTCTGGTCCC+CGG - chr4.3:4980726-4980745 None:intergenic 50.0%
ATGGAGGACGAAGAGCTCTG+TGG - chr4.3:4980989-4981008 None:intergenic 55.0%
CAGAGCTCTTCGTCCTCCAT+TGG + chr4.3:4980989-4981008 MS.gene55767:CDS 55.0%
CAGTTCGGTTGCATTTGCCG+AGG - chr4.3:4980770-4980789 None:intergenic 55.0%
CGGGGACCAGAAAGCAACAT+AGG + chr4.3:4980724-4980743 MS.gene55767:CDS 55.0%
GCAACCGAACTGTCATGCGA+TGG + chr4.3:4980778-4980797 MS.gene55767:CDS 55.0%
GTACAAGGCCCGCAAAGAGA+AGG + chr4.3:4980956-4980975 MS.gene55767:CDS 55.0%
TGGAGGACGAAGAGCTCTGT+GGG - chr4.3:4980988-4981007 None:intergenic 55.0%
!! ACGCTAGTGAGGGGTATTCC+GGG + chr4.3:4980705-4980724 MS.gene55767:CDS 55.0%
CAAGGCCCGCAAAGAGAAGG+AGG + chr4.3:4980959-4980978 MS.gene55767:CDS 60.0%
!! CGCTAGTGAGGGGTATTCCG+GGG + chr4.3:4980706-4980725 MS.gene55767:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 4980636 4981043 4980636 ID=MS.gene55767
chr4.3 mRNA 4980636 4981043 4980636 ID=MS.gene55767.t1;Parent=MS.gene55767
chr4.3 exon 4980636 4980833 4980636 ID=MS.gene55767.t1.exon1;Parent=MS.gene55767.t1
chr4.3 CDS 4980636 4980833 4980636 ID=cds.MS.gene55767.t1;Parent=MS.gene55767.t1
chr4.3 exon 4980921 4981043 4980921 ID=MS.gene55767.t1.exon2;Parent=MS.gene55767.t1
chr4.3 CDS 4980921 4981043 4980921 ID=cds.MS.gene55767.t1;Parent=MS.gene55767.t1
Gene Sequence

>MS.gene55767

ATGAGCATGAATGCGTTCAAGGCATATAAGTCCTGTGTCCCAATTGCTTATAGCCCCAATCTCTACATAACGCTAGTGAGGGGTATTCCGGGGACCAGAAAGCAACATAGGCGCACTTTGGAAGCACTACGCCTCGGCAAATGCAACCGAACTGTCATGCGATGGAACACACCTACTGTTAGGGGAATGATCCAGCAGGTTTCAGCACAAGATCTATCAGATCCATTTTGAAACTACCTATCTCCCTGTATACTAACAACATTGGTAACTTTGTATTTGATATAGGTCAAGAGGTTAGTTGTTGTTGAGACAGAGGAGATGTACAAGGCCCGCAAAGAGAAGGAGGAAACCCACAGAGCTCTTCGTCCTCCATTGGTCATAAACCATCAACCTGCTTCTTCTGCTTAG

Protein sequence

>MS.gene55767.t1

MSMNAFKAYKSCVPIAYSPNLYITLVRGIPGTRKQHRRTLEALRLGKCNRTVMRWNTPTVRGMIQQVKRLVVVETEEMYKARKEKEETHRALRPPLVINHQPASSA