Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56561.t1 | XP_003588480.1 | 95.2 | 207 | 10 | 0 | 1 | 207 | 83 | 289 | 7.90E-89 | 336.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56561.t1 | Q9FNE9 | 73.7 | 209 | 53 | 2 | 1 | 207 | 88 | 296 | 2.0e-64 | 246.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56561.t1 | G7I3E6 | 95.2 | 207 | 10 | 0 | 1 | 207 | 83 | 289 | 5.7e-89 | 336.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049989 | MS.gene56561 | 0.836576 | 8.63E-57 | -1.69E-46 |
| MS.gene051816 | MS.gene56561 | 0.834414 | 3.04E-56 | -1.69E-46 |
| MS.gene052308 | MS.gene56561 | 0.801859 | 7.38E-49 | -1.69E-46 |
| MS.gene052367 | MS.gene56561 | 0.854291 | 1.36E-61 | -1.69E-46 |
| MS.gene053173 | MS.gene56561 | 0.801736 | 7.82E-49 | -1.69E-46 |
| MS.gene053416 | MS.gene56561 | 0.820318 | 7.31E-53 | -1.69E-46 |
| MS.gene053417 | MS.gene56561 | 0.821527 | 3.85E-53 | -1.69E-46 |
| MS.gene054551 | MS.gene56561 | 0.821394 | 4.13E-53 | -1.69E-46 |
| MS.gene054593 | MS.gene56561 | 0.800251 | 1.57E-48 | -1.69E-46 |
| MS.gene055578 | MS.gene56561 | 0.832553 | 8.85E-56 | -1.69E-46 |
| MS.gene055957 | MS.gene56561 | 0.828431 | 9.02E-55 | -1.69E-46 |
| MS.gene056041 | MS.gene56561 | 0.860117 | 2.58E-63 | -1.69E-46 |
| MS.gene05640 | MS.gene56561 | 0.818386 | 2.01E-52 | -1.69E-46 |
| MS.gene057458 | MS.gene56561 | 0.826017 | 3.42E-54 | -1.69E-46 |
| MS.gene058196 | MS.gene56561 | 0.80636 | 8.54E-50 | -1.69E-46 |
| MS.gene059553 | MS.gene56561 | 0.804075 | 2.57E-49 | -1.69E-46 |
| MS.gene060473 | MS.gene56561 | 0.848881 | 4.62E-60 | -1.69E-46 |
| MS.gene060565 | MS.gene56561 | 0.837387 | 5.36E-57 | -1.69E-46 |
| MS.gene06093 | MS.gene56561 | 0.830464 | 2.89E-55 | -1.69E-46 |
| MS.gene061099 | MS.gene56561 | 0.824299 | 8.70E-54 | -1.69E-46 |
| MS.gene061260 | MS.gene56561 | 0.838676 | 2.50E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATATTGCTACGTTTATC+TGG | 0.121333 | 1.3:+11812217 | None:intergenic |
| CTTGAAGAAATCAATAATTT+TGG | 0.167292 | 1.3:+11814195 | None:intergenic |
| AATATAGCACGTTTCATTAA+TGG | 0.211173 | 1.3:-11812202 | MS.gene56561:CDS |
| GCATCCCTTGGCGCCATTCC+AGG | 0.276840 | 1.3:+11813424 | None:intergenic |
| CTCCTCTTCAAATCATCATT+TGG | 0.347535 | 1.3:+11813523 | None:intergenic |
| GAGAAAGCGGAGTAGTAGTT+TGG | 0.397233 | 1.3:-11813584 | MS.gene56561:CDS |
| GTTGTTTATGATCCTGTAGA+AGG | 0.406850 | 1.3:-11812482 | MS.gene56561:CDS |
| ACGTTCAAGAACAGGAGAAT+TGG | 0.452094 | 1.3:+11813402 | None:intergenic |
| ATTCCTCCACGTTCAAGAAC+AGG | 0.452190 | 1.3:+11813394 | None:intergenic |
| AAAGCGGAGTAGTAGTTTGG+TGG | 0.462327 | 1.3:-11813581 | MS.gene56561:CDS |
| CGCGTTGACTGCTACTAAAA+CGG | 0.476912 | 1.3:-11813473 | MS.gene56561:CDS |
| CTATATTGCTACGTTTATCT+GGG | 0.481139 | 1.3:+11812218 | None:intergenic |
| ACAATAATAACAGAATATGT+TGG | 0.490743 | 1.3:-11812337 | MS.gene56561:CDS |
| TAACTTGTGCAGAAGTATGA+TGG | 0.493269 | 1.3:-11812531 | MS.gene56561:CDS |
| TTCTAATCCTTCACAGTCAC+TGG | 0.505311 | 1.3:-11812248 | MS.gene56561:CDS |
| GCATCTAAGATTCTGAATCA+TGG | 0.512650 | 1.3:-11814154 | MS.gene56561:intron |
| ATAAGTACTTACCCTTCTAC+AGG | 0.515050 | 1.3:+11812470 | None:intergenic |
| ATTAGAACAGATGGCGTCGT+TGG | 0.518768 | 1.3:-11813500 | MS.gene56561:CDS |
| AATGAGCTTACGTACATGCC+TGG | 0.527386 | 1.3:-11813442 | MS.gene56561:CDS |
| ATTCAAAGAAGAAGAGAAAG+CGG | 0.540362 | 1.3:-11813597 | MS.gene56561:CDS |
| ACAGGAGAATTGGCATCCCT+TGG | 0.541184 | 1.3:+11813412 | None:intergenic |
| TCTTGAACGTGGAGGAATGC+AGG | 0.541405 | 1.3:-11813389 | MS.gene56561:intron |
| AAGAATCGGGAATATGATGA+TGG | 0.544853 | 1.3:-11812298 | MS.gene56561:CDS |
| AGGAGAATGTCCGCCCCTCA+TGG | 0.545962 | 1.3:-11812504 | MS.gene56561:CDS |
| TGCAGAAGTATGATGGAAAG+AGG | 0.546783 | 1.3:-11812524 | MS.gene56561:CDS |
| AATTCTCCTGTTCTTGAACG+TGG | 0.561001 | 1.3:-11813400 | MS.gene56561:CDS |
| CATGCCTGGAATGGCGCCAA+GGG | 0.564637 | 1.3:-11813428 | MS.gene56561:CDS |
| ACATGCCTGGAATGGCGCCA+AGG | 0.588883 | 1.3:-11813429 | MS.gene56561:CDS |
| CAGAATCTTAGATGCATCAA+CGG | 0.589395 | 1.3:+11814162 | None:intergenic |
| TGCCAAATGATGATTTGAAG+AGG | 0.592036 | 1.3:-11813525 | MS.gene56561:CDS |
| GAAGAGGAGATTAGAACAGA+TGG | 0.593117 | 1.3:-11813509 | MS.gene56561:CDS |
| GCTTACGTACATGCCTGGAA+TGG | 0.609175 | 1.3:-11813437 | MS.gene56561:CDS |
| TTGTTTATGATCCTGTAGAA+GGG | 0.614616 | 1.3:-11812481 | MS.gene56561:intron |
| TCTGCACAAGTTAAGAGTCT+CGG | 0.617054 | 1.3:+11812541 | None:intergenic |
| TCTCCTGTTCTTGAACGTGG+AGG | 0.653986 | 1.3:-11813397 | MS.gene56561:CDS |
| GGATCATAAACAACCATGAG+GGG | 0.675550 | 1.3:+11812491 | None:intergenic |
| AGGATCATAAACAACCATGA+GGG | 0.678750 | 1.3:+11812490 | None:intergenic |
| TCATAAACAACCATGAGGGG+CGG | 0.687468 | 1.3:+11812494 | None:intergenic |
| CAGGATCATAAACAACCATG+AGG | 0.703912 | 1.3:+11812489 | None:intergenic |
| CAGATGACCAGTGACTGTGA+AGG | 0.718767 | 1.3:+11812241 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTATTGTCAAATCTTTTA+TGG | + | chr1.3:11814049-11814068 | None:intergenic | 15.0% |
| !!! | TCTAATTTTCTTTTTTTTGT+AGG | - | chr1.3:11814001-11814020 | MS.gene56561:intron | 15.0% |
| !! | AAAAAAGAAAATTAGAAGTC+AGG | + | chr1.3:11813997-11814016 | None:intergenic | 20.0% |
| !! | ACAATAATAACAGAATATGT+TGG | - | chr1.3:11814060-11814079 | MS.gene56561:intron | 20.0% |
| !! | TAATGTTTAGAGAATTTGAT+TGG | - | chr1.3:11812305-11812324 | MS.gene56561:CDS | 20.0% |
| !! | TTAATTGAAAAATTCTACTG+TGG | - | chr1.3:11813161-11813180 | MS.gene56561:intron | 20.0% |
| !!! | CTTGAAGAAATCAATAATTT+TGG | + | chr1.3:11812205-11812224 | None:intergenic | 20.0% |
| !!! | TTAGGTATTATATTGTTGTT+AGG | - | chr1.3:11813060-11813079 | MS.gene56561:intron | 20.0% |
| ! | AAAAAAGAAAAGGAAGTTGT+TGG | - | chr1.3:11812843-11812862 | MS.gene56561:intron | 25.0% |
| ! | AATATAGCACGTTTCATTAA+TGG | - | chr1.3:11814195-11814214 | MS.gene56561:CDS | 25.0% |
| !! | ATAACTTTTGTAAGACTGTT+TGG | + | chr1.3:11812694-11812713 | None:intergenic | 25.0% |
| !! | TTAGGTTGTCAATACTAATT+AGG | - | chr1.3:11813398-11813417 | MS.gene56561:CDS | 25.0% |
| !!! | ATAATTTTGGTTTGTACAAG+AGG | + | chr1.3:11812192-11812211 | None:intergenic | 25.0% |
| !!! | ATGTAGACTTTTTGAAGAAT+CGG | - | chr1.3:11814085-11814104 | MS.gene56561:intron | 25.0% |
| !!! | CTTAATTGAGTTTGTTAGTT+AGG | - | chr1.3:11813042-11813061 | MS.gene56561:intron | 25.0% |
| !!! | GTTTGCATATATTTTTTCTC+AGG | - | chr1.3:11813815-11813834 | MS.gene56561:intron | 25.0% |
| !!! | TATTGTTGTTAGGATACTTT+TGG | - | chr1.3:11813070-11813089 | MS.gene56561:intron | 25.0% |
| ACAATAGTGAAAACAGTTGA+TGG | + | chr1.3:11812603-11812622 | None:intergenic | 30.0% | |
| ATTCAAAGAAGAAGAGAAAG+CGG | - | chr1.3:11812800-11812819 | MS.gene56561:intron | 30.0% | |
| CTATATTGCTACGTTTATCT+GGG | + | chr1.3:11814182-11814201 | None:intergenic | 30.0% | |
| GTTTAGAGAATTTGATTGGA+TGG | - | chr1.3:11812309-11812328 | MS.gene56561:CDS | 30.0% | |
| TGAGACATTGAATTGATTGT+TGG | - | chr1.3:11813287-11813306 | MS.gene56561:intron | 30.0% | |
| TGCAATACTGAAGTATAAAG+TGG | - | chr1.3:11813642-11813661 | MS.gene56561:intron | 30.0% | |
| TGGTGGTTTCAAAAAAGAAA+AGG | - | chr1.3:11812833-11812852 | MS.gene56561:intron | 30.0% | |
| TGTTGAGTTATGTTTGTATG+AGG | - | chr1.3:11813133-11813152 | MS.gene56561:intron | 30.0% | |
| TTGTTTATGATCCTGTAGAA+GGG | - | chr1.3:11813916-11813935 | MS.gene56561:intron | 30.0% | |
| ! | ACTTTGTTGCCTTTATTTCT+AGG | + | chr1.3:11813621-11813640 | None:intergenic | 30.0% |
| ! | TCTGTTTTCACAAGCTATTA+TGG | - | chr1.3:11812476-11812495 | MS.gene56561:intron | 30.0% |
| !! | AGTTTATACTTTTTGAGAGC+TGG | - | chr1.3:11813456-11813475 | MS.gene56561:CDS | 30.0% |
| !! | CTACAAACTAGTTTGGATTT+TGG | - | chr1.3:11813670-11813689 | MS.gene56561:intron | 30.0% |
| !! | CTATTTTTCACAACCTATTG+TGG | - | chr1.3:11812547-11812566 | MS.gene56561:CDS | 30.0% |
| !! | GAAGTGTGTGTTTTAAATTG+TGG | - | chr1.3:11812770-11812789 | MS.gene56561:intron | 30.0% |
| !! | TGTAGACTTTTTGAAGAATC+GGG | - | chr1.3:11814086-11814105 | MS.gene56561:intron | 30.0% |
| !! | TTGTTTTGTTGAACTGATAC+TGG | - | chr1.3:11813375-11813394 | MS.gene56561:intron | 30.0% |
| !!! | GCTGTTTTTACAAGTTATTG+TGG | - | chr1.3:11812367-11812386 | MS.gene56561:CDS | 30.0% |
| AAGAATCGGGAATATGATGA+TGG | - | chr1.3:11814099-11814118 | MS.gene56561:intron | 35.0% | |
| AGGATCATAAACAACCATGA+GGG | + | chr1.3:11813910-11813929 | None:intergenic | 35.0% | |
| ATAAGTACTTACCCTTCTAC+AGG | + | chr1.3:11813930-11813949 | None:intergenic | 35.0% | |
| CAGAATCTTAGATGCATCAA+CGG | + | chr1.3:11812238-11812257 | None:intergenic | 35.0% | |
| CTCCTCTTCAAATCATCATT+TGG | + | chr1.3:11812877-11812896 | None:intergenic | 35.0% | |
| GACATTGAATTGATTGTTGG+TGG | - | chr1.3:11813290-11813309 | MS.gene56561:intron | 35.0% | |
| GAGAATTTGATTGGATGGAT+TGG | - | chr1.3:11812314-11812333 | MS.gene56561:CDS | 35.0% | |
| GCAGTGTTACCTAGAAATAA+AGG | - | chr1.3:11813609-11813628 | MS.gene56561:intron | 35.0% | |
| GCTATATTGCTACGTTTATC+TGG | + | chr1.3:11814183-11814202 | None:intergenic | 35.0% | |
| GCTCTGATAATCTGATTAAG+TGG | + | chr1.3:11813747-11813766 | None:intergenic | 35.0% | |
| GTTGTTTATGATCCTGTAGA+AGG | - | chr1.3:11813915-11813934 | MS.gene56561:intron | 35.0% | |
| TAATACAAGCGCAATGTCTT+TGG | - | chr1.3:11813509-11813528 | MS.gene56561:CDS | 35.0% | |
| TCGTCTTCATTTGTACAACT+AGG | + | chr1.3:11813431-11813450 | None:intergenic | 35.0% | |
| TCTCAACTTATGCTCAATGT+AGG | - | chr1.3:11813534-11813553 | MS.gene56561:CDS | 35.0% | |
| TGCCAAATGATGATTTGAAG+AGG | - | chr1.3:11812872-11812891 | MS.gene56561:intron | 35.0% | |
| ! | GCATCTAAGATTCTGAATCA+TGG | - | chr1.3:11812243-11812262 | MS.gene56561:CDS | 35.0% |
| ! | TAACTTGTGCAGAAGTATGA+TGG | - | chr1.3:11813866-11813885 | MS.gene56561:intron | 35.0% |
| ! | TGGTTTGTACAAGAGGAAAA+GGG | + | chr1.3:11812185-11812204 | None:intergenic | 35.0% |
| ! | TTGTTGAACTGATACTGGTT+AGG | - | chr1.3:11813380-11813399 | MS.gene56561:intron | 35.0% |
| !! | GCTGTTTTCATAAGCTAATG+TGG | - | chr1.3:11812436-11812455 | MS.gene56561:intron | 35.0% |
| !! | TTATACTTTTTGAGAGCTGG+TGG | - | chr1.3:11813459-11813478 | MS.gene56561:CDS | 35.0% |
| !! | TTGATTGGATGGATTGGTTT+GGG | - | chr1.3:11812320-11812339 | MS.gene56561:CDS | 35.0% |
| !! | TTGGTTTGTACAAGAGGAAA+AGG | + | chr1.3:11812186-11812205 | None:intergenic | 35.0% |
| !! | TTTGATTGGATGGATTGGTT+TGG | - | chr1.3:11812319-11812338 | MS.gene56561:CDS | 35.0% |
| AAGTTATTGTGGAGAGCTTG+TGG | - | chr1.3:11812378-11812397 | MS.gene56561:intron | 40.0% | |
| AATTCTCCTGTTCTTGAACG+TGG | - | chr1.3:11812997-11813016 | MS.gene56561:intron | 40.0% | |
| ACGTTCAAGAACAGGAGAAT+TGG | + | chr1.3:11812998-11813017 | None:intergenic | 40.0% | |
| CAGGATCATAAACAACCATG+AGG | + | chr1.3:11813911-11813930 | None:intergenic | 40.0% | |
| CTGCACTAGTTGTCATGATT+TGG | - | chr1.3:11813697-11813716 | MS.gene56561:intron | 40.0% | |
| GAAGAGGAGATTAGAACAGA+TGG | - | chr1.3:11812888-11812907 | MS.gene56561:intron | 40.0% | |
| GGATCATAAACAACCATGAG+GGG | + | chr1.3:11813909-11813928 | None:intergenic | 40.0% | |
| GGTCTGTCTACAAACTAGTT+TGG | - | chr1.3:11813663-11813682 | MS.gene56561:intron | 40.0% | |
| TCTGCACAAGTTAAGAGTCT+CGG | + | chr1.3:11813859-11813878 | None:intergenic | 40.0% | |
| TTCTAATCCTTCACAGTCAC+TGG | - | chr1.3:11814149-11814168 | MS.gene56561:intron | 40.0% | |
| TTTCACATGCTCTCCACAAT+AGG | + | chr1.3:11812563-11812582 | None:intergenic | 40.0% | |
| ! | TGCAGAAGTATGATGGAAAG+AGG | - | chr1.3:11813873-11813892 | MS.gene56561:intron | 40.0% |
| AAAGCGGAGTAGTAGTTTGG+TGG | - | chr1.3:11812816-11812835 | MS.gene56561:intron | 45.0% | |
| AATGAGCTTACGTACATGCC+TGG | - | chr1.3:11812955-11812974 | MS.gene56561:intron | 45.0% | |
| ATGTTGCCTCTTTGTCGTCA+TGG | - | chr1.3:11813255-11813274 | MS.gene56561:intron | 45.0% | |
| ATTAGAACAGATGGCGTCGT+TGG | - | chr1.3:11812897-11812916 | MS.gene56561:intron | 45.0% | |
| ATTCCTCCACGTTCAAGAAC+AGG | + | chr1.3:11813006-11813025 | None:intergenic | 45.0% | |
| GAGAAAGCGGAGTAGTAGTT+TGG | - | chr1.3:11812813-11812832 | MS.gene56561:intron | 45.0% | |
| TCATAAACAACCATGAGGGG+CGG | + | chr1.3:11813906-11813925 | None:intergenic | 45.0% | |
| TCTAGGTAACACTGCGTACT+AGG | + | chr1.3:11813604-11813623 | None:intergenic | 45.0% | |
| TCTTAGCCATGACGACAAAG+AGG | + | chr1.3:11813264-11813283 | None:intergenic | 45.0% | |
| TTGCATCTCAGTGTGCATGT+TGG | - | chr1.3:11813102-11813121 | MS.gene56561:intron | 45.0% | |
| ! | CAGTGTGCATGTTGGTTGTT+TGG | - | chr1.3:11813110-11813129 | MS.gene56561:intron | 45.0% |
| !! | CGCGTTGACTGCTACTAAAA+CGG | - | chr1.3:11812924-11812943 | MS.gene56561:intron | 45.0% |
| !! | CGTTTTAGTAGCAGTCAACG+CGG | + | chr1.3:11812926-11812945 | None:intergenic | 45.0% |
| ACAGGAGAATTGGCATCCCT+TGG | + | chr1.3:11812988-11813007 | None:intergenic | 50.0% | |
| CAGATGACCAGTGACTGTGA+AGG | + | chr1.3:11814159-11814178 | None:intergenic | 50.0% | |
| GCTTACGTACATGCCTGGAA+TGG | - | chr1.3:11812960-11812979 | MS.gene56561:intron | 50.0% | |
| TCTCCTGTTCTTGAACGTGG+AGG | - | chr1.3:11813000-11813019 | MS.gene56561:intron | 50.0% | |
| TCTTGAACGTGGAGGAATGC+AGG | - | chr1.3:11813008-11813027 | MS.gene56561:intron | 50.0% | |
| ACATGCCTGGAATGGCGCCA+AGG | - | chr1.3:11812968-11812987 | MS.gene56561:intron | 60.0% | |
| AGGAGAATGTCCGCCCCTCA+TGG | - | chr1.3:11813893-11813912 | MS.gene56561:intron | 60.0% | |
| CATGCCTGGAATGGCGCCAA+GGG | - | chr1.3:11812969-11812988 | MS.gene56561:intron | 60.0% | |
| GCATCCCTTGGCGCCATTCC+AGG | + | chr1.3:11812976-11812995 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 11812184 | 11814235 | 11812184 | ID=MS.gene56561 |
| chr1.3 | mRNA | 11812184 | 11814235 | 11812184 | ID=MS.gene56561.t1;Parent=MS.gene56561 |
| chr1.3 | exon | 11814155 | 11814235 | 11814155 | ID=MS.gene56561.t1.exon1;Parent=MS.gene56561.t1 |
| chr1.3 | CDS | 11814155 | 11814235 | 11814155 | ID=cds.MS.gene56561.t1;Parent=MS.gene56561.t1 |
| chr1.3 | exon | 11813390 | 11813619 | 11813390 | ID=MS.gene56561.t1.exon2;Parent=MS.gene56561.t1 |
| chr1.3 | CDS | 11813390 | 11813619 | 11813390 | ID=cds.MS.gene56561.t1;Parent=MS.gene56561.t1 |
| chr1.3 | exon | 11812482 | 11812582 | 11812482 | ID=MS.gene56561.t1.exon3;Parent=MS.gene56561.t1 |
| chr1.3 | CDS | 11812482 | 11812582 | 11812482 | ID=cds.MS.gene56561.t1;Parent=MS.gene56561.t1 |
| chr1.3 | exon | 11812184 | 11812396 | 11812184 | ID=MS.gene56561.t1.exon4;Parent=MS.gene56561.t1 |
| chr1.3 | CDS | 11812184 | 11812396 | 11812184 | ID=cds.MS.gene56561.t1;Parent=MS.gene56561.t1 |
| Gene Sequence |
| Protein sequence |
