AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56727

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56727.t1 MTR_6g009520 95.174 518 25 0 1 518 1 518 0.0 947
MS.gene56727.t1 MTR_6g009540 87.016 516 67 0 3 518 1 516 0.0 860
MS.gene56727.t1 MTR_6g009720 73.123 506 116 5 1 493 1 499 0.0 713
MS.gene56727.t1 MTR_6g009553 75.664 452 91 3 25 461 1 448 0.0 690
MS.gene56727.t1 MTR_6g009770 71.400 507 103 7 1 494 1 478 0.0 676
MS.gene56727.t1 MTR_7g008040 67.171 463 143 4 5 461 1 460 0.0 642
MS.gene56727.t1 MTR_6g042290 69.723 469 121 4 12 479 21 469 0.0 640
MS.gene56727.t1 MTR_6g009510 66.270 504 94 5 11 513 20 448 0.0 605
MS.gene56727.t1 MTR_4g107370 58.607 488 185 7 7 480 8 492 0.0 587
MS.gene56727.t1 MTR_6g009660 62.235 519 125 10 3 510 1 459 0.0 583
MS.gene56727.t1 MTR_1g022250 59.708 479 174 6 3 477 1 464 0.0 580
MS.gene56727.t1 MTR_2g030420 57.872 470 181 5 7 472 8 464 0.0 558
MS.gene56727.t1 MTR_1g022245 52.155 464 206 5 7 460 3 460 2.54e-172 496
MS.gene56727.t1 MTR_4g128260 44.695 443 233 5 7 441 3 441 1.08e-127 383
MS.gene56727.t1 MTR_5g020980 38.855 489 257 8 8 484 13 471 2.67e-116 353
MS.gene56727.t1 MTR_2g076970 39.271 494 255 10 12 501 7 459 8.49e-111 337
MS.gene56727.t1 MTR_2g076710 40.977 471 226 13 14 470 14 446 1.42e-110 336
MS.gene56727.t1 MTR_2g061820 37.931 493 277 11 5 491 4 473 8.89e-109 334
MS.gene56727.t1 MTR_2g076920 39.516 496 247 12 12 499 13 463 8.40e-108 330
MS.gene56727.t1 MTR_2g076690 40.289 484 231 13 14 482 16 456 5.34e-107 327
MS.gene56727.t1 MTR_8g076920 38.285 478 264 9 8 481 23 473 2.55e-106 327
MS.gene56727.t1 MTR_2g076840 41.202 466 233 10 12 472 7 436 4.80e-106 333
MS.gene56727.t1 MTR_2g076840 49.296 284 112 6 180 461 450 703 9.95e-85 277
MS.gene56727.t1 MTR_2g076900 38.958 480 258 9 12 485 7 457 3.71e-104 320
MS.gene56727.t1 MTR_2g076990 42.092 411 202 9 65 469 1 381 4.57e-102 314
MS.gene56727.t1 MTR_5g020990 37.603 484 267 12 3 472 19 481 1.61e-95 298
MS.gene56727.t1 MTR_3g103150 44.702 302 138 4 162 462 4 277 4.25e-84 262
MS.gene56727.t1 MTR_3g092060 30.342 468 257 12 8 461 2 414 8.68e-67 224
MS.gene56727.t1 MTR_7g117950 32.921 404 209 11 63 461 59 405 1.44e-65 220
MS.gene56727.t1 MTR_5g021020 38.365 318 149 7 146 462 135 406 9.54e-59 200
MS.gene56727.t1 MTR_8g027200 28.404 426 229 12 40 461 35 388 4.79e-51 181
MS.gene56727.t1 MTR_1g011680 60.000 135 49 3 328 460 2 133 5.30e-42 148
MS.gene56727.t1 MTR_5g021000 53.211 109 47 2 355 462 1 106 2.56e-31 119
MS.gene56727.t1 MTR_5g020720 42.105 171 79 5 268 437 25 176 6.33e-28 110
MS.gene56727.t1 MTR_1g113750 50.000 108 52 2 388 494 16 122 4.58e-22 92.4
MS.gene56727.t1 MTR_5g021010 49.451 91 42 2 372 461 34 121 3.82e-18 80.9
MS.gene56727.t1 MTR_1g083330 31.250 208 109 7 221 418 180 363 8.59e-14 73.9
MS.gene56727.t1 MTR_4g033235 31.250 208 109 7 221 418 181 364 1.08e-13 73.6
MS.gene56727.t1 MTR_6g059690 27.673 318 178 14 188 480 177 467 4.84e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56727.t1 AT5G40010 62.391 460 163 5 5 457 4 460 0.0 591
MS.gene56727.t1 AT3G28580 60.954 461 163 8 5 457 4 455 0.0 552
MS.gene56727.t1 AT3G28510 59.219 461 186 2 6 466 5 463 0.0 535
MS.gene56727.t1 AT3G28580 57.642 458 161 7 5 457 4 433 5.75e-177 507
MS.gene56727.t1 AT3G28540 57.298 459 183 6 6 459 5 455 6.97e-173 498
MS.gene56727.t1 AT3G28540 57.298 459 183 6 6 459 5 455 9.79e-173 497
MS.gene56727.t1 AT3G28610 50.936 481 177 10 10 465 4 450 3.80e-166 479
MS.gene56727.t1 AT3G28600 51.148 479 191 8 1 465 1 450 4.07e-164 474
MS.gene56727.t1 AT5G40000 48.361 488 209 9 3 474 2 462 6.16e-156 453
MS.gene56727.t1 AT3G28520 55.556 477 197 7 17 487 2 469 3.58e-155 451
MS.gene56727.t1 AT3G28600 48.225 479 166 8 1 465 1 411 1.32e-145 426
MS.gene56727.t1 AT3G28570 47.009 468 197 8 10 464 7 436 1.22e-144 424
MS.gene56727.t1 AT3G28570 47.009 468 197 8 10 464 14 443 1.24e-144 424
MS.gene56727.t1 AT5G57480 39.961 508 281 9 7 503 3 497 6.45e-126 378
MS.gene56727.t1 AT4G25835 41.053 475 267 7 7 477 3 468 2.40e-124 374
MS.gene56727.t1 AT4G30250 40.466 472 259 8 3 462 1 462 3.11e-115 351
MS.gene56727.t1 AT4G30250 40.426 470 258 8 5 462 1 460 6.87e-115 350
MS.gene56727.t1 AT3G50930 39.308 491 268 11 20 504 73 539 4.11e-109 337
MS.gene56727.t1 AT3G50940 39.421 449 227 9 28 471 43 451 1.18e-108 332
MS.gene56727.t1 AT2G18193 39.407 472 253 8 13 479 19 462 6.68e-107 328
MS.gene56727.t1 AT5G17740 36.647 513 277 13 6 495 11 498 3.92e-103 320
MS.gene56727.t1 AT4G05380 38.854 471 255 10 4 468 11 454 1.65e-101 313
MS.gene56727.t1 AT5G17730 36.382 492 266 12 5 482 10 468 3.35e-101 313
MS.gene56727.t1 AT5G17760 40.000 445 213 9 68 487 72 487 7.37e-101 313
MS.gene56727.t1 AT2G18190 38.696 460 251 9 8 464 15 446 1.52e-99 309
MS.gene56727.t1 AT1G43910 38.266 473 262 10 4 471 10 457 2.05e-96 301
MS.gene56727.t1 AT5G17750 33.800 429 223 8 8 426 13 390 4.10e-83 263
MS.gene56727.t1 AT2G46620 29.878 492 294 8 5 491 1 446 5.19e-71 235
MS.gene56727.t1 AT5G17760 37.786 262 137 4 68 314 72 322 1.50e-52 182
MS.gene56727.t1 AT3G28560 43.119 218 114 4 6 221 5 214 5.46e-49 170
MS.gene56727.t1 AT3G29800 31.562 320 169 7 143 458 104 377 5.19e-45 164
MS.gene56727.t1 AT4G05340 50.000 96 42 1 317 412 4 93 3.40e-23 94.4
MS.gene56727.t1 AT1G09100 32.632 190 95 6 221 401 180 345 3.75e-14 75.1
MS.gene56727.t1 AT3G05530 31.250 208 109 7 221 418 181 364 5.58e-14 74.3
MS.gene56727.t1 AT3G02450 25.649 308 177 11 224 519 346 613 4.64e-12 68.9

Find 77 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGTAAGGTAACTCTTTC+TGG 0.086559 6.2:-5066086 MS.gene56727:CDS
AAACGTGGTTATCTACTTTA+TGG 0.185533 6.2:-5066380 MS.gene56727:CDS
CAAAGTATGAAGATGGGAAT+TGG 0.285135 6.2:-5067118 None:intergenic
TTTGAGCATCCTGCAAGATT+TGG 0.292624 6.2:-5066503 MS.gene56727:CDS
CAAATAACCCTAGTAGTAAT+TGG 0.316335 6.2:-5066562 MS.gene56727:CDS
ATAAAGTTTCATGAATTATC+AGG 0.317330 6.2:-5066950 MS.gene56727:CDS
TGAAGAGGAAGCAAGGAAAA+AGG 0.331891 6.2:-5065712 MS.gene56727:CDS
AAAGTATGAAGATGGGAATT+GGG 0.332092 6.2:-5067117 None:intergenic
ATGGTAGATTTCCCAGTTCC+TGG 0.332105 6.2:+5066353 None:intergenic
AGAAAATATCTACGAAGATT+AGG 0.335237 6.2:+5067019 None:intergenic
TCTTGAGGAAGCAAAGAAAA+AGG 0.342972 6.2:-5065736 MS.gene56727:CDS
CTTTATGGTCCTCCAGGAAC+TGG 0.355152 6.2:-5066365 MS.gene56727:CDS
TCCAATTATTGAAAAGTTGT+TGG 0.390561 6.2:-5065856 MS.gene56727:CDS
AAAGAATATTATGCTAAAGT+TGG 0.397678 6.2:-5066413 MS.gene56727:CDS
CCAATTATTGAAAAGTTGTT+GGG 0.400795 6.2:-5065855 MS.gene56727:CDS
GCTAGGGTTCCAAATCTTGC+AGG 0.402544 6.2:+5066494 None:intergenic
AGTTCAATGGAGTCAAAGTT+TGG 0.406209 6.2:-5066772 MS.gene56727:CDS
TGAAACTTTATGTGGATGTA+TGG 0.409252 6.2:+5066962 None:intergenic
AACATTTCAAAGTATGAAGA+TGG 0.422358 6.2:-5067125 None:intergenic
AAAAGCTTGATAGCTACAAT+AGG 0.435837 6.2:+5065929 None:intergenic
AAATCTTCAGTGGTTGACTT+TGG 0.445094 6.2:+5065789 None:intergenic
AAAAGGATGAAGAGGAAGCA+AGG 0.445932 6.2:-5065719 MS.gene56727:CDS
AGTTTGCACTAAGATATGTT+TGG 0.446804 6.2:+5066891 None:intergenic
CTTCCCCTTCTTATAAGAGC+AGG 0.448315 6.2:+5065975 None:intergenic
CTCTTATATCCAACATGTGT+TGG 0.448653 6.2:-5066633 MS.gene56727:CDS
GATCTTCTTAAGTTCAAGAA+AGG 0.458025 6.2:-5066437 MS.gene56727:CDS
AGATGGGAATTGGGGAACTA+TGG 0.460698 6.2:-5067108 MS.gene56727:CDS
ATTGACTCAAGAGGAAGAAA+AGG 0.464359 6.2:-5065643 MS.gene56727:CDS
CAACACATGTTGGATATAAG+AGG 0.468856 6.2:+5066634 None:intergenic
ATGCTAAAGTTGGAAAGGCT+TGG 0.473908 6.2:-5066403 MS.gene56727:CDS
TTGATCCTGCTCTTATAAGA+AGG 0.478149 6.2:-5065980 MS.gene56727:CDS
GAGGAAAAGGTGAAGGAGAA+TGG 0.482194 6.2:-5065600 MS.gene56727:CDS
AAGCTATTATGAGCAAGAAT+AGG 0.495148 6.2:-5066601 MS.gene56727:CDS
ATCCAACATGTGTTGGATGA+AGG 0.498679 6.2:-5066626 MS.gene56727:CDS
CTCTCGATCTCACCGGGCAA+AGG 0.502496 6.2:-5066190 MS.gene56727:CDS
TATCTACTTTATGGTCCTCC+AGG 0.505261 6.2:-5066371 MS.gene56727:CDS
GATGGGATTTGGTCAGCTTG+TGG 0.509504 6.2:-5066047 MS.gene56727:CDS
GAAAGAAGAATTGACTCAAG+AGG 0.510872 6.2:-5065652 MS.gene56727:CDS
GATTGTTCTCTCGATCTCAC+CGG 0.518830 6.2:-5066197 MS.gene56727:CDS
ATAATTCATGAAACTTTATG+TGG 0.520872 6.2:+5066954 None:intergenic
TCTTGAATTAACAACTGTAA+AGG 0.523071 6.2:-5066288 MS.gene56727:CDS
TGATCCTGCTCTTATAAGAA+GGG 0.525975 6.2:-5065979 MS.gene56727:CDS
AAATTCTCAGCAACATCAGC+AGG 0.526229 6.2:+5065816 None:intergenic
ATTTGCAATGGCTGATATCA+TGG 0.530323 6.2:+5066334 None:intergenic
GAAATCATAGATGAGTTCAA+TGG 0.530945 6.2:-5066785 MS.gene56727:CDS
GAGAATGGTGTCAAAGAGAA+TGG 0.531190 6.2:-5065585 MS.gene56727:CDS
GACTTTGATTCAATCTCTTG+AGG 0.533207 6.2:-5065751 MS.gene56727:CDS
TCTTATAAGAAGGGGAAGAA+TGG 0.536532 6.2:-5065970 MS.gene56727:CDS
ATTGTTCTCTCGATCTCACC+GGG 0.546404 6.2:-5066196 MS.gene56727:CDS
TAACCCTAGTAGTAATTGGT+GGG 0.547641 6.2:-5066558 MS.gene56727:CDS
AAATATCTACGAAGATTAGG+TGG 0.547924 6.2:+5067022 None:intergenic
GCAACTTTCAAAATCTTCAG+TGG 0.552936 6.2:+5065779 None:intergenic
AAAATTAGAGGAAAAGGTGA+AGG 0.554515 6.2:-5065607 MS.gene56727:CDS
TTCCTTCATCCAACACATGT+TGG 0.555695 6.2:+5066624 None:intergenic
AAAACCACTTGTTCTTAGCA+TGG 0.557204 6.2:-5066819 MS.gene56727:CDS
TGATGAGAATGATGAAAAGA+AGG 0.571153 6.2:-5066144 MS.gene56727:CDS
AACCCTAGTAGTAATTGGTG+GGG 0.572012 6.2:-5066557 MS.gene56727:CDS
TCATCCATGCTAAGAACAAG+TGG 0.573558 6.2:+5066815 None:intergenic
ATATTATGCTAAAGTTGGAA+AGG 0.575311 6.2:-5066408 MS.gene56727:CDS
ATAGTTCATGAAATTTGCAA+TGG 0.585085 6.2:+5066322 None:intergenic
AAGTATGAAGATGGGAATTG+GGG 0.585836 6.2:-5067116 None:intergenic
TCAATGGAGTCAAAGTTTGG+TGG 0.596166 6.2:-5066769 MS.gene56727:CDS
ACATTTCAAAGTATGAAGAT+GGG 0.598757 6.2:-5067124 None:intergenic
GTTGGAAAGGCTTGGAAACG+TGG 0.601459 6.2:-5066395 MS.gene56727:CDS
TTTATGGTCCTCCAGGAACT+GGG 0.602895 6.2:-5066364 MS.gene56727:CDS
GGATGAGGAAGCAAAGCTCA+AGG 0.616773 6.2:-5065685 MS.gene56727:CDS
GAGAATGGTGTGAAAGAGAA+CGG 0.617159 6.2:-5065570 MS.gene56727:CDS
ACCCTAGTAGTAATTGGTGG+GGG 0.617964 6.2:-5066556 MS.gene56727:CDS
GGGGAACTATGGTCACAACT+AGG 0.620126 6.2:-5067097 MS.gene56727:CDS
ATAACCCTAGTAGTAATTGG+TGG 0.620183 6.2:-5066559 MS.gene56727:CDS
AAGATTTGGAACCCTAGCTA+TGG 0.624596 6.2:-5066489 MS.gene56727:CDS
AATATCTACGAAGATTAGGT+GGG 0.627753 6.2:+5067023 None:intergenic
GTCACAACTAGGTTCAATCA+CGG 0.654145 6.2:-5067086 MS.gene56727:CDS
GATCCTGCTCTTATAAGAAG+GGG 0.655948 6.2:-5065978 MS.gene56727:CDS
TCAAGAGGAAGAAAAGGTGA+AGG 0.669369 6.2:-5065637 MS.gene56727:CDS
GTAGATTTCCCAGTTCCTGG+AGG 0.699693 6.2:+5066356 None:intergenic
GGTCAGCTTGTGGATCAGAG+AGG 0.714357 6.2:-5066037 MS.gene56727:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAATATTATGCTAAAGT+TGG - chr6.2:5066256-5066275 MS.gene56727:CDS 20.0%
!! ATAAAGTTTCATGAATTATC+AGG - chr6.2:5065719-5065738 MS.gene56727:CDS 20.0%
!! ATAATTCATGAAACTTTATG+TGG + chr6.2:5065718-5065737 None:intergenic 20.0%
!!! AATTTTCACAACAAATTTTG+TGG - chr6.2:5066660-5066679 MS.gene56727:CDS 20.0%
!!! GTTTGTTGAATTTTATTGAT+GGG - chr6.2:5066605-5066624 MS.gene56727:CDS 20.0%
! AGAAAATATCTACGAAGATT+AGG + chr6.2:5065653-5065672 None:intergenic 25.0%
! AGAAGAGAAAAATGAAAGTA+AGG - chr6.2:5066570-5066589 MS.gene56727:CDS 25.0%
! ATAGTTCATGAAATTTGCAA+TGG + chr6.2:5066350-5066369 None:intergenic 25.0%
! ATATTATGCTAAAGTTGGAA+AGG - chr6.2:5066261-5066280 MS.gene56727:CDS 25.0%
! CCAATTATTGAAAAGTTGTT+GGG - chr6.2:5066814-5066833 MS.gene56727:CDS 25.0%
! TCCAATTATTGAAAAGTTGT+TGG - chr6.2:5066813-5066832 MS.gene56727:CDS 25.0%
! TCTTGAATTAACAACTGTAA+AGG - chr6.2:5066381-5066400 MS.gene56727:CDS 25.0%
! TGATGAAAAATTAGAGGAAA+AGG - chr6.2:5067056-5067075 MS.gene56727:CDS 25.0%
!! AGAAAATGATTGTGATTTTG+AGG + chr6.2:5065942-5065961 None:intergenic 25.0%
!! TGAATTTTATTGATGGGATT+TGG - chr6.2:5066611-5066630 MS.gene56727:CDS 25.0%
!!! AAAATCTCTTGTTTCTTTTC+TGG + chr6.2:5066208-5066227 None:intergenic 25.0%
!!! AATCTTTTTTCATCTGAAGT+AGG + chr6.2:5065971-5065990 None:intergenic 25.0%
!!! GGTTTGTTGAATTTTATTGA+TGG - chr6.2:5066604-5066623 MS.gene56727:CDS 25.0%
!!! TCTTTTTCAGCTTTCTTAAT+AGG + chr6.2:5066553-5066572 None:intergenic 25.0%
AAAATTAGAGGAAAAGGTGA+AGG - chr6.2:5067062-5067081 MS.gene56727:CDS 30.0%
AAACGTGGTTATCTACTTTA+TGG - chr6.2:5066289-5066308 MS.gene56727:CDS 30.0%
AAATATCTACGAAGATTAGG+TGG + chr6.2:5065650-5065669 None:intergenic 30.0%
AAGCTATTATGAGCAAGAAT+AGG - chr6.2:5066068-5066087 MS.gene56727:CDS 30.0%
AATATCTACGAAGATTAGGT+GGG + chr6.2:5065649-5065668 None:intergenic 30.0%
AGTTTGCACTAAGATATGTT+TGG + chr6.2:5065781-5065800 None:intergenic 30.0%
CAAATAACCCTAGTAGTAAT+TGG - chr6.2:5066107-5066126 MS.gene56727:CDS 30.0%
GAAATCATAGATGAGTTCAA+TGG - chr6.2:5065884-5065903 MS.gene56727:CDS 30.0%
GATCTTCTTAAGTTCAAGAA+AGG - chr6.2:5066232-5066251 MS.gene56727:CDS 30.0%
TGAAACTTTATGTGGATGTA+TGG + chr6.2:5065710-5065729 None:intergenic 30.0%
TGATGAGAATGATGAAAAGA+AGG - chr6.2:5066525-5066544 MS.gene56727:CDS 30.0%
TGGGAAAAATTTGTCAAACA+TGG + chr6.2:5065630-5065649 None:intergenic 30.0%
! AAAAGCTTGATAGCTACAAT+AGG + chr6.2:5066743-5066762 None:intergenic 30.0%
! ATTGTAGCTATCAAGCTTTT+AGG - chr6.2:5066743-5066762 MS.gene56727:CDS 30.0%
! CCCAACAACTTTTCAATAAT+TGG + chr6.2:5066817-5066836 None:intergenic 30.0%
! TTGTAGCTATCAAGCTTTTA+GGG - chr6.2:5066744-5066763 MS.gene56727:CDS 30.0%
!! AAATGATTGTGATTTTGAGG+TGG + chr6.2:5065939-5065958 None:intergenic 30.0%
!! GAAGGTTGATGAAAAATTAG+AGG - chr6.2:5067050-5067069 MS.gene56727:CDS 30.0%
AAAACCACTTGTTCTTAGCA+TGG - chr6.2:5065850-5065869 MS.gene56727:CDS 35.0%
AGCAAAGAAAAAGGATGAAG+AGG - chr6.2:5066942-5066961 MS.gene56727:CDS 35.0%
AGTTCAATGGAGTCAAAGTT+TGG - chr6.2:5065897-5065916 MS.gene56727:CDS 35.0%
ATAACCCTAGTAGTAATTGG+TGG - chr6.2:5066110-5066129 MS.gene56727:CDS 35.0%
ATTTGCAATGGCTGATATCA+TGG + chr6.2:5066338-5066357 None:intergenic 35.0%
CAACACATGTTGGATATAAG+AGG + chr6.2:5066038-5066057 None:intergenic 35.0%
CTCTTATATCCAACATGTGT+TGG - chr6.2:5066036-5066055 MS.gene56727:CDS 35.0%
GAAAGTAAGGTAACTCTTTC+TGG - chr6.2:5066583-5066602 MS.gene56727:CDS 35.0%
GACTTTGATTCAATCTCTTG+AGG - chr6.2:5066918-5066937 MS.gene56727:CDS 35.0%
GCAACTTTCAAAATCTTCAG+TGG + chr6.2:5066893-5066912 None:intergenic 35.0%
TAACCCTAGTAGTAATTGGT+GGG - chr6.2:5066111-5066130 MS.gene56727:CDS 35.0%
TCTTATAAGAAGGGGAAGAA+TGG - chr6.2:5066699-5066718 MS.gene56727:CDS 35.0%
TCTTGAGGAAGCAAAGAAAA+AGG - chr6.2:5066933-5066952 MS.gene56727:CDS 35.0%
TGATCCTGCTCTTATAAGAA+GGG - chr6.2:5066690-5066709 MS.gene56727:CDS 35.0%
TTGATCCTGCTCTTATAAGA+AGG - chr6.2:5066689-5066708 MS.gene56727:CDS 35.0%
! AAATCTTCAGTGGTTGACTT+TGG + chr6.2:5066883-5066902 None:intergenic 35.0%
! ATTGACTCAAGAGGAAGAAA+AGG - chr6.2:5067026-5067045 MS.gene56727:CDS 35.0%
! GAAAGAAGAATTGACTCAAG+AGG - chr6.2:5067017-5067036 MS.gene56727:CDS 35.0%
! TGCTAAGAACAAGTGGTTTT+TGG + chr6.2:5065850-5065869 None:intergenic 35.0%
!! ATGCTTTTTGTGGAAGGTTA+GGG + chr6.2:5065999-5066018 None:intergenic 35.0%
!!! TTTTTTCTTCTTCCTTTGCC+CGG + chr6.2:5066494-5066513 None:intergenic 35.0%
AAAAGGATGAAGAGGAAGCA+AGG - chr6.2:5066950-5066969 MS.gene56727:CDS 40.0%
AAATTCTCAGCAACATCAGC+AGG + chr6.2:5066856-5066875 None:intergenic 40.0%
AACCCTAGTAGTAATTGGTG+GGG - chr6.2:5066112-5066131 MS.gene56727:CDS 40.0%
AAGGAAAAAGGTAGAGGATG+AGG - chr6.2:5066969-5066988 MS.gene56727:CDS 40.0%
ATCCAACATGTGTTGGATGA+AGG - chr6.2:5066043-5066062 MS.gene56727:CDS 40.0%
ATGCTAAAGTTGGAAAGGCT+TGG - chr6.2:5066266-5066285 MS.gene56727:CDS 40.0%
GAGAATGGTGTGAAAGAGAA+CGG - chr6.2:5067099-5067118 MS.gene56727:CDS 40.0%
GATCCTGCTCTTATAAGAAG+GGG - chr6.2:5066691-5066710 MS.gene56727:CDS 40.0%
GTCACAACTAGGTTCAATCA+CGG - chr6.2:5065583-5065602 MS.gene56727:CDS 40.0%
TATCTACTTTATGGTCCTCC+AGG - chr6.2:5066298-5066317 MS.gene56727:CDS 40.0%
TCAAGAGGAAGAAAAGGTGA+AGG - chr6.2:5067032-5067051 MS.gene56727:CDS 40.0%
TCAATGGAGTCAAAGTTTGG+TGG - chr6.2:5065900-5065919 MS.gene56727:CDS 40.0%
TCATCCATGCTAAGAACAAG+TGG + chr6.2:5065857-5065876 None:intergenic 40.0%
TGAAGAGGAAGCAAGGAAAA+AGG - chr6.2:5066957-5066976 MS.gene56727:CDS 40.0%
TTCCTTCATCCAACACATGT+TGG + chr6.2:5066048-5066067 None:intergenic 40.0%
TTTGAGCATCCTGCAAGATT+TGG - chr6.2:5066166-5066185 MS.gene56727:CDS 40.0%
! AAGATTTGGAACCCTAGCTA+TGG - chr6.2:5066180-5066199 MS.gene56727:CDS 40.0%
! GAGAATGGTGTCAAAGAGAA+TGG - chr6.2:5067084-5067103 MS.gene56727:CDS 40.0%
!! GATGCTTTTTGTGGAAGGTT+AGG + chr6.2:5066000-5066019 None:intergenic 40.0%
!! TGAGTTCACGATGCTTTTTG+TGG + chr6.2:5066009-5066028 None:intergenic 40.0%
!! TTCACGATGCTTTTTGTGGA+AGG + chr6.2:5066005-5066024 None:intergenic 40.0%
!!! CTTTTCTGGTTCCATAGCTA+GGG + chr6.2:5066194-5066213 None:intergenic 40.0%
!!! TCTTTTCTGGTTCCATAGCT+AGG + chr6.2:5066195-5066214 None:intergenic 40.0%
ACCCCCACCAATTACTACTA+GGG + chr6.2:5066117-5066136 None:intergenic 45.0%
ACCCTAGTAGTAATTGGTGG+GGG - chr6.2:5066113-5066132 MS.gene56727:CDS 45.0%
AGATGGGAATTGGGGAACTA+TGG - chr6.2:5065561-5065580 MS.gene56727:CDS 45.0%
ATGGTAGATTTCCCAGTTCC+TGG + chr6.2:5066319-5066338 None:intergenic 45.0%
ATTGTTCTCTCGATCTCACC+GGG - chr6.2:5066473-5066492 MS.gene56727:CDS 45.0%
CTTCCCCTTCTTATAAGAGC+AGG + chr6.2:5066697-5066716 None:intergenic 45.0%
GAGGAAAAGGTGAAGGAGAA+TGG - chr6.2:5067069-5067088 MS.gene56727:CDS 45.0%
GATTGTTCTCTCGATCTCAC+CGG - chr6.2:5066472-5066491 MS.gene56727:CDS 45.0%
GGAAGCAAGGAAAAAGGTAG+AGG - chr6.2:5066963-5066982 MS.gene56727:CDS 45.0%
TACCCCCACCAATTACTACT+AGG + chr6.2:5066118-5066137 None:intergenic 45.0%
TTTATGGTCCTCCAGGAACT+GGG - chr6.2:5066305-5066324 MS.gene56727:CDS 45.0%
! GGGGGTATAAAAGCACAAAG+TGG - chr6.2:5066131-5066150 MS.gene56727:CDS 45.0%
CTTTATGGTCCTCCAGGAAC+TGG - chr6.2:5066304-5066323 MS.gene56727:CDS 50.0%
GCTAGGGTTCCAAATCTTGC+AGG + chr6.2:5066178-5066197 None:intergenic 50.0%
GGATGAGGAAGCAAAGCTCA+AGG - chr6.2:5066984-5067003 MS.gene56727:CDS 50.0%
GGGGAACTATGGTCACAACT+AGG - chr6.2:5065572-5065591 MS.gene56727:CDS 50.0%
GTAGATTTCCCAGTTCCTGG+AGG + chr6.2:5066316-5066335 None:intergenic 50.0%
GTTGGAAAGGCTTGGAAACG+TGG - chr6.2:5066274-5066293 MS.gene56727:CDS 50.0%
! GATGGGATTTGGTCAGCTTG+TGG - chr6.2:5066622-5066641 MS.gene56727:CDS 50.0%
GGTCAGCTTGTGGATCAGAG+AGG - chr6.2:5066632-5066651 MS.gene56727:CDS 55.0%
CTCTCGATCTCACCGGGCAA+AGG - chr6.2:5066479-5066498 MS.gene56727:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 5065557 5067134 5065557 ID=MS.gene56727
chr6.2 mRNA 5065557 5067134 5065557 ID=MS.gene56727.t1;Parent=MS.gene56727
chr6.2 exon 5065557 5067134 5065557 ID=MS.gene56727.t1.exon1;Parent=MS.gene56727.t1
chr6.2 CDS 5065557 5067134 5065557 ID=cds.MS.gene56727.t1;Parent=MS.gene56727.t1
Gene Sequence

>MS.gene56727

ATGAAGATGGGAATTGGGGAACTATGGTCACAACTAGGTTCAATCACGGCTAGCATAATGTTTGTGTATGCCATGTTTGACAAATTTTTCCCACCTAATCTTCGTAGATATTTTCTAAAATACACAAACAAATTCACAAACTTTATGTATCCATACATCCACATAAAGTTTCATGAATTATCAGGAGAACGTCTCAAACAAAGTGAAACTTACAAAATCATCCAAACATATCTTAGTGCAAACTCATCACAAAGAGCAAGAAGACTTAAAGCTGAAGTTGTCAAAGATAGCCAAAAACCACTTGTTCTTAGCATGGATGACAATGAAGAAATCATAGATGAGTTCAATGGAGTCAAAGTTTGGTGGACTGCTAATTACACCACCTCAAAATCACAATCATTTTCTTACTATCCTACTTCAGATGAAAAAAGATTCTTAACCCTAACCTTCCACAAAAAGCATCGTGAACTCATCACTACCTCTTATATCCAACATGTGTTGGATGAAGGAAAAGCTATTATGAGCAAGAATAGGCAACTAAAGCTTTACACAAATAACCCTAGTAGTAATTGGTGGGGGTATAAAAGCACAAAGTGGAATCACACAACTTTTGAGCATCCTGCAAGATTTGGAACCCTAGCTATGGAACCAGAAAAGAAACAAGAGATTTTAAATGATCTTCTTAAGTTCAAGAAAGGTAAAGAATATTATGCTAAAGTTGGAAAGGCTTGGAAACGTGGTTATCTACTTTATGGTCCTCCAGGAACTGGGAAATCTACCATGATATCAGCCATTGCAAATTTCATGAACTATGATGTGTATGATCTTGAATTAACAACTGTAAAGGATAACAATGAGTTGAAGAGACTCTTGATTGAGACGTCGAGTAAATCGATTATAGTTATCGAAGATATTGATTGTTCTCTCGATCTCACCGGGCAAAGGAAGAAGAAAAAAGAGAAAGATGATGATGAGAATGATGAAAAGAAGGATCCTATTAAGAAAGCTGAAAAAGAAGAGAAAAATGAAAGTAAGGTAACTCTTTCTGGTTTGTTGAATTTTATTGATGGGATTTGGTCAGCTTGTGGATCAGAGAGGATCATAATTTTCACAACAAATTTTGTGGACAAACTTGATCCTGCTCTTATAAGAAGGGGAAGAATGGATAAGCACATTGAAATGTCCTATTGTAGCTATCAAGCTTTTAGGGTTCTTGCAAGAAACTACTTAGATGTTGAGTCTCATGAAGATTTGTTTCCAATTATTGAAAAGTTGTTGGGAGAGACTAATATGACACCTGCTGATGTTGCTGAGAATTTGATGCCAAAGTCAACCACTGAAGATTTTGAAAGTTGCTTGAAGACTTTGATTCAATCTCTTGAGGAAGCAAAGAAAAAGGATGAAGAGGAAGCAAGGAAAAAGGTAGAGGATGAGGAAGCAAAGCTCAAGGCAGAGAAAGAGAAAGAAGAATTGACTCAAGAGGAAGAAAAGGTGAAGGTTGATGAAAAATTAGAGGAAAAGGTGAAGGAGAATGGTGTCAAAGAGAATGGTGTGAAAGAGAACGGTGTCATCACTTAA
Protein sequence

>MS.gene56727.t1

MKMGIGELWSQLGSITASIMFVYAMFDKFFPPNLRRYFLKYTNKFTNFMYPYIHIKFHELSGERLKQSETYKIIQTYLSANSSQRARRLKAEVVKDSQKPLVLSMDDNEEIIDEFNGVKVWWTANYTTSKSQSFSYYPTSDEKRFLTLTFHKKHRELITTSYIQHVLDEGKAIMSKNRQLKLYTNNPSSNWWGYKSTKWNHTTFEHPARFGTLAMEPEKKQEILNDLLKFKKGKEYYAKVGKAWKRGYLLYGPPGTGKSTMISAIANFMNYDVYDLELTTVKDNNELKRLLIETSSKSIIVIEDIDCSLDLTGQRKKKKEKDDDENDEKKDPIKKAEKEEKNESKVTLSGLLNFIDGIWSACGSERIIIFTTNFVDKLDPALIRRGRMDKHIEMSYCSYQAFRVLARNYLDVESHEDLFPIIEKLLGETNMTPADVAENLMPKSTTEDFESCLKTLIQSLEEAKKKDEEEARKKVEDEEAKLKAEKEKEELTQEEEKVKVDEKLEEKVKENGVKENGVKENGVIT