Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58273.t1 | XP_013441780.1 | 84.8 | 230 | 32 | 1 | 1 | 227 | 7 | 236 | 1.60E-104 | 389 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58273.t1 | Q2V6J9 | 36.7 | 240 | 138 | 4 | 1 | 227 | 1 | 239 | 5.2e-35 | 149.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58273.t1 | A0A072TQH5 | 84.8 | 230 | 32 | 1 | 1 | 227 | 7 | 236 | 1.2e-104 | 389.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049828 | MS.gene58273 | 0.86969 | 2.56E-66 | -1.69E-46 |
| MS.gene049907 | MS.gene58273 | 0.873567 | 1.33E-67 | -1.69E-46 |
| MS.gene050520 | MS.gene58273 | 0.8196 | 1.07E-52 | -1.69E-46 |
| MS.gene050581 | MS.gene58273 | 0.85782 | 1.26E-62 | -1.69E-46 |
| MS.gene050767 | MS.gene58273 | 0.812331 | 4.47E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene58273 | 0.810353 | 1.20E-50 | -1.69E-46 |
| MS.gene051255 | MS.gene58273 | 0.812878 | 3.40E-51 | -1.69E-46 |
| MS.gene053813 | MS.gene58273 | 0.809296 | 2.03E-50 | -1.69E-46 |
| MS.gene054376 | MS.gene58273 | 0.801136 | 1.04E-48 | -1.69E-46 |
| MS.gene055499 | MS.gene58273 | 0.81228 | 4.59E-51 | -1.69E-46 |
| MS.gene056611 | MS.gene58273 | 0.832709 | 8.10E-56 | -1.69E-46 |
| MS.gene056942 | MS.gene58273 | 0.819948 | 8.88E-53 | -1.69E-46 |
| MS.gene058459 | MS.gene58273 | 0.810501 | 1.12E-50 | -1.69E-46 |
| MS.gene058903 | MS.gene58273 | 0.82715 | 1.83E-54 | -1.69E-46 |
| MS.gene059711 | MS.gene58273 | 0.826361 | 2.83E-54 | -1.69E-46 |
| MS.gene059974 | MS.gene58273 | 0.812256 | 4.65E-51 | -1.69E-46 |
| MS.gene060005 | MS.gene58273 | 0.813058 | 3.10E-51 | -1.69E-46 |
| MS.gene060356 | MS.gene58273 | 0.857237 | 1.87E-62 | -1.69E-46 |
| MS.gene060801 | MS.gene58273 | 0.805809 | 1.12E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene58273 | 0.808824 | 2.56E-50 | -1.69E-46 |
| MS.gene061134 | MS.gene58273 | 0.817511 | 3.17E-52 | -1.69E-46 |
| MS.gene061200 | MS.gene58273 | 0.817478 | 3.23E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58273.t1 | MTR_0536s0020 | 84.783 | 230 | 32 | 1 | 1 | 227 | 7 | 236 | 1.12e-139 | 401 |
| MS.gene58273.t1 | MTR_0536s0020 | 65.306 | 49 | 17 | 0 | 204 | 252 | 437 | 485 | 2.01e-12 | 66.6 |
| MS.gene58273.t1 | MTR_4g117890 | 84.186 | 215 | 34 | 0 | 1 | 215 | 2 | 216 | 1.49e-132 | 383 |
| MS.gene58273.t1 | MTR_0536s0030 | 87.006 | 177 | 23 | 0 | 36 | 212 | 4 | 180 | 2.12e-108 | 317 |
| MS.gene58273.t1 | MTR_0536s0030 | 86.486 | 37 | 5 | 0 | 216 | 252 | 321 | 357 | 1.73e-13 | 69.7 |
| MS.gene58273.t1 | MTR_4g118000 | 62.054 | 224 | 79 | 3 | 7 | 227 | 7 | 227 | 1.69e-90 | 275 |
| MS.gene58273.t1 | MTR_0536s0010 | 55.133 | 263 | 96 | 4 | 8 | 252 | 17 | 275 | 2.80e-89 | 273 |
| MS.gene58273.t1 | MTR_4g117950 | 52.852 | 263 | 103 | 4 | 8 | 252 | 2 | 261 | 6.49e-85 | 261 |
| MS.gene58273.t1 | MTR_4g117980 | 50.644 | 233 | 102 | 4 | 8 | 227 | 7 | 239 | 6.26e-75 | 236 |
| MS.gene58273.t1 | MTR_4g117960 | 51.389 | 216 | 97 | 2 | 8 | 215 | 7 | 222 | 6.61e-74 | 233 |
| MS.gene58273.t1 | MTR_2g034990 | 51.835 | 218 | 95 | 2 | 8 | 215 | 7 | 224 | 2.03e-72 | 229 |
| MS.gene58273.t1 | MTR_2g035020 | 49.541 | 218 | 100 | 2 | 8 | 215 | 7 | 224 | 2.41e-70 | 224 |
| MS.gene58273.t1 | MTR_6g035265 | 44.906 | 265 | 123 | 4 | 8 | 252 | 7 | 268 | 1.93e-68 | 219 |
| MS.gene58273.t1 | MTR_5g029800 | 48.918 | 231 | 105 | 3 | 8 | 227 | 7 | 235 | 2.63e-67 | 216 |
| MS.gene58273.t1 | MTR_7g012120 | 47.186 | 231 | 111 | 3 | 8 | 227 | 12 | 242 | 8.73e-65 | 209 |
| MS.gene58273.t1 | MTR_5g067170 | 47.845 | 232 | 108 | 4 | 8 | 227 | 7 | 237 | 3.51e-64 | 208 |
| MS.gene58273.t1 | MTR_8g032950 | 46.983 | 232 | 113 | 3 | 6 | 227 | 6 | 237 | 2.88e-60 | 197 |
| MS.gene58273.t1 | MTR_6g035185 | 44.068 | 236 | 113 | 4 | 8 | 227 | 7 | 239 | 3.82e-57 | 189 |
| MS.gene58273.t1 | MTR_4g031800 | 39.906 | 213 | 121 | 2 | 7 | 212 | 4 | 216 | 2.57e-52 | 177 |
| MS.gene58273.t1 | MTR_4g117970 | 44.937 | 158 | 80 | 2 | 77 | 227 | 53 | 210 | 4.38e-42 | 148 |
| MS.gene58273.t1 | MTR_7g070660 | 36.771 | 223 | 119 | 4 | 11 | 214 | 10 | 229 | 1.26e-39 | 143 |
| MS.gene58273.t1 | MTR_4g485630 | 34.322 | 236 | 139 | 5 | 7 | 227 | 4 | 238 | 3.69e-39 | 142 |
| MS.gene58273.t1 | MTR_6g035295 | 36.515 | 241 | 133 | 6 | 5 | 225 | 5 | 245 | 1.34e-38 | 140 |
| MS.gene58273.t1 | MTR_7g070610 | 35.398 | 226 | 124 | 4 | 11 | 217 | 12 | 234 | 2.20e-38 | 140 |
| MS.gene58273.t1 | MTR_7g070780 | 37.727 | 220 | 121 | 4 | 11 | 214 | 10 | 229 | 3.64e-38 | 139 |
| MS.gene58273.t1 | MTR_7g070740 | 34.978 | 223 | 123 | 4 | 11 | 214 | 10 | 229 | 8.36e-38 | 138 |
| MS.gene58273.t1 | MTR_7g070930 | 37.273 | 220 | 122 | 4 | 11 | 214 | 13 | 232 | 1.12e-37 | 138 |
| MS.gene58273.t1 | MTR_7g055710 | 35.455 | 220 | 126 | 4 | 11 | 214 | 10 | 229 | 2.66e-37 | 137 |
| MS.gene58273.t1 | MTR_7g071040 | 36.364 | 220 | 124 | 3 | 11 | 214 | 16 | 235 | 3.13e-37 | 137 |
| MS.gene58273.t1 | MTR_7g070800 | 34.956 | 226 | 125 | 4 | 8 | 214 | 2 | 224 | 8.69e-37 | 135 |
| MS.gene58273.t1 | MTR_5g016660 | 35.088 | 228 | 132 | 3 | 3 | 214 | 1 | 228 | 1.15e-36 | 135 |
| MS.gene58273.t1 | MTR_7g070860 | 33.480 | 227 | 129 | 4 | 7 | 214 | 2 | 225 | 2.66e-36 | 134 |
| MS.gene58273.t1 | MTR_7g070810 | 36.123 | 227 | 129 | 3 | 7 | 217 | 4 | 230 | 4.70e-36 | 133 |
| MS.gene58273.t1 | MTR_7g070940 | 35.088 | 228 | 131 | 4 | 3 | 214 | 1 | 227 | 5.54e-36 | 133 |
| MS.gene58273.t1 | MTR_4g108250 | 36.910 | 233 | 127 | 7 | 1 | 214 | 1 | 232 | 3.25e-35 | 131 |
| MS.gene58273.t1 | MTR_8g066610 | 32.883 | 222 | 129 | 3 | 11 | 212 | 8 | 229 | 4.73e-35 | 130 |
| MS.gene58273.t1 | MTR_7g071050 | 39.891 | 183 | 105 | 3 | 1 | 178 | 1 | 183 | 1.80e-34 | 128 |
| MS.gene58273.t1 | MTR_5g039900 | 32.231 | 242 | 144 | 5 | 3 | 224 | 2 | 243 | 2.47e-34 | 129 |
| MS.gene58273.t1 | MTR_7g070910 | 33.184 | 223 | 133 | 4 | 11 | 217 | 10 | 232 | 3.67e-34 | 128 |
| MS.gene58273.t1 | MTR_4g485620 | 32.864 | 213 | 117 | 4 | 24 | 213 | 1 | 210 | 2.21e-32 | 123 |
| MS.gene58273.t1 | MTR_7g117405 | 31.674 | 221 | 133 | 5 | 17 | 221 | 17 | 235 | 3.08e-32 | 123 |
| MS.gene58273.t1 | MTR_7g070870 | 33.772 | 228 | 138 | 3 | 3 | 217 | 10 | 237 | 5.75e-32 | 122 |
| MS.gene58273.t1 | MTR_5g045970 | 33.937 | 221 | 127 | 6 | 11 | 212 | 15 | 235 | 3.21e-31 | 120 |
| MS.gene58273.t1 | MTR_1g040310 | 35.169 | 236 | 135 | 5 | 7 | 225 | 10 | 244 | 7.78e-31 | 119 |
| MS.gene58273.t1 | MTR_7g102560 | 30.667 | 225 | 139 | 6 | 7 | 214 | 2 | 226 | 2.70e-30 | 117 |
| MS.gene58273.t1 | MTR_5g040030 | 32.083 | 240 | 144 | 6 | 5 | 225 | 7 | 246 | 1.66e-29 | 115 |
| MS.gene58273.t1 | MTR_7g102450 | 31.507 | 219 | 117 | 5 | 17 | 217 | 16 | 219 | 6.99e-29 | 114 |
| MS.gene58273.t1 | MTR_5g045960 | 30.342 | 234 | 142 | 6 | 7 | 221 | 4 | 235 | 9.65e-29 | 113 |
| MS.gene58273.t1 | MTR_1g062380 | 32.203 | 236 | 139 | 7 | 11 | 225 | 15 | 250 | 9.68e-29 | 114 |
| MS.gene58273.t1 | MTR_4g485590 | 35.652 | 230 | 112 | 6 | 13 | 227 | 10 | 218 | 4.54e-28 | 110 |
| MS.gene58273.t1 | MTR_2g035050 | 54.206 | 107 | 47 | 1 | 8 | 112 | 7 | 113 | 9.26e-28 | 103 |
| MS.gene58273.t1 | MTR_7g102550 | 30.000 | 260 | 158 | 7 | 15 | 252 | 12 | 269 | 1.08e-27 | 110 |
| MS.gene58273.t1 | MTR_4g485640 | 34.975 | 203 | 110 | 5 | 22 | 212 | 219 | 411 | 1.99e-27 | 110 |
| MS.gene58273.t1 | MTR_2g035040 | 44.860 | 107 | 51 | 1 | 117 | 215 | 23 | 129 | 3.06e-27 | 108 |
| MS.gene58273.t1 | MTR_8g044140 | 35.263 | 190 | 106 | 4 | 40 | 213 | 5 | 193 | 7.49e-26 | 105 |
| MS.gene58273.t1 | MTR_7g102490 | 31.799 | 239 | 125 | 10 | 12 | 221 | 7 | 236 | 9.99e-26 | 105 |
| MS.gene58273.t1 | MTR_4g024020 | 31.624 | 234 | 133 | 9 | 12 | 221 | 7 | 237 | 2.59e-25 | 104 |
| MS.gene58273.t1 | MTR_7g039570 | 33.514 | 185 | 113 | 4 | 5 | 179 | 7 | 191 | 4.76e-25 | 100 |
| MS.gene58273.t1 | MTR_7g102520 | 28.837 | 215 | 136 | 6 | 11 | 208 | 11 | 225 | 4.40e-24 | 100 |
| MS.gene58273.t1 | MTR_7g102460 | 29.412 | 238 | 131 | 10 | 12 | 221 | 7 | 235 | 6.53e-24 | 100 |
| MS.gene58273.t1 | MTR_7g470590 | 45.217 | 115 | 58 | 3 | 11 | 120 | 10 | 124 | 6.21e-21 | 85.5 |
| MS.gene58273.t1 | MTR_7g070570 | 45.217 | 115 | 58 | 3 | 11 | 120 | 10 | 124 | 6.21e-21 | 85.5 |
| MS.gene58273.t1 | MTR_2g098430 | 28.736 | 174 | 112 | 5 | 13 | 175 | 7 | 179 | 3.74e-16 | 77.8 |
| MS.gene58273.t1 | MTR_0009s0240 | 29.358 | 218 | 134 | 8 | 13 | 213 | 8 | 222 | 3.18e-14 | 72.0 |
| MS.gene58273.t1 | MTR_5g087620 | 26.702 | 191 | 121 | 6 | 3 | 175 | 1 | 190 | 4.18e-13 | 68.9 |
| MS.gene58273.t1 | MTR_8g068340 | 34.899 | 149 | 90 | 4 | 1 | 146 | 1 | 145 | 5.89e-13 | 68.2 |
| MS.gene58273.t1 | MTR_7g113320 | 31.496 | 127 | 72 | 5 | 97 | 208 | 25 | 151 | 1.86e-12 | 65.1 |
| MS.gene58273.t1 | MTR_8g068360 | 35.256 | 156 | 91 | 6 | 1 | 153 | 1 | 149 | 1.08e-11 | 64.7 |
| MS.gene58273.t1 | MTR_8g068330 | 32.609 | 138 | 90 | 3 | 7 | 143 | 3 | 138 | 3.91e-11 | 62.8 |
| MS.gene58273.t1 | MTR_7g067120 | 28.767 | 146 | 97 | 4 | 9 | 151 | 6 | 147 | 7.90e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58273.t1 | AT2G15490 | 32.700 | 263 | 152 | 5 | 13 | 250 | 8 | 270 | 2.39e-38 | 135 |
| MS.gene58273.t1 | AT4G34131 | 34.061 | 229 | 124 | 5 | 9 | 212 | 7 | 233 | 2.65e-37 | 137 |
| MS.gene58273.t1 | AT4G34135 | 32.114 | 246 | 138 | 5 | 9 | 231 | 8 | 247 | 1.31e-36 | 132 |
| MS.gene58273.t1 | AT4G34135 | 32.114 | 246 | 138 | 5 | 9 | 231 | 8 | 247 | 8.24e-36 | 133 |
| MS.gene58273.t1 | AT2G15490 | 34.802 | 227 | 121 | 5 | 11 | 212 | 6 | 230 | 1.70e-34 | 129 |
| MS.gene58273.t1 | AT2G15490 | 34.802 | 227 | 121 | 5 | 11 | 212 | 6 | 230 | 1.99e-34 | 129 |
| MS.gene58273.t1 | AT2G15480 | 34.222 | 225 | 125 | 5 | 11 | 212 | 9 | 233 | 4.16e-34 | 128 |
| MS.gene58273.t1 | AT2G15480 | 34.222 | 225 | 125 | 5 | 11 | 212 | 9 | 233 | 4.43e-34 | 128 |
| MS.gene58273.t1 | AT4G34138 | 32.751 | 229 | 122 | 6 | 12 | 212 | 9 | 233 | 2.04e-30 | 118 |
| MS.gene58273.t1 | AT3G53150 | 32.287 | 223 | 132 | 5 | 9 | 212 | 19 | 241 | 2.90e-30 | 118 |
| MS.gene58273.t1 | AT2G36780 | 29.204 | 226 | 133 | 5 | 10 | 212 | 12 | 233 | 1.61e-29 | 115 |
| MS.gene58273.t1 | AT2G36760 | 28.054 | 221 | 140 | 6 | 11 | 212 | 13 | 233 | 7.61e-29 | 114 |
| MS.gene58273.t1 | AT2G36800 | 27.801 | 241 | 152 | 6 | 1 | 221 | 1 | 239 | 8.26e-29 | 114 |
| MS.gene58273.t1 | AT3G53160 | 33.047 | 233 | 131 | 8 | 11 | 219 | 7 | 238 | 8.62e-29 | 114 |
| MS.gene58273.t1 | AT2G36770 | 28.959 | 221 | 138 | 4 | 11 | 212 | 13 | 233 | 1.26e-28 | 113 |
| MS.gene58273.t1 | AT2G36790 | 28.458 | 253 | 159 | 6 | 1 | 233 | 1 | 251 | 2.35e-28 | 112 |
| MS.gene58273.t1 | AT2G36750 | 31.004 | 229 | 138 | 6 | 11 | 221 | 9 | 235 | 3.82e-28 | 112 |
| MS.gene58273.t1 | AT2G16890 | 27.731 | 238 | 147 | 8 | 13 | 226 | 10 | 246 | 5.48e-16 | 76.3 |
| MS.gene58273.t1 | AT2G16890 | 27.731 | 238 | 147 | 8 | 13 | 226 | 10 | 246 | 5.48e-16 | 76.3 |
| MS.gene58273.t1 | AT2G16890 | 27.803 | 223 | 137 | 7 | 13 | 212 | 10 | 231 | 6.15e-16 | 77.0 |
| MS.gene58273.t1 | AT2G16890 | 27.803 | 223 | 137 | 7 | 13 | 212 | 10 | 231 | 8.61e-16 | 76.6 |
| MS.gene58273.t1 | AT5G14860 | 25.322 | 233 | 136 | 7 | 15 | 213 | 11 | 239 | 1.03e-15 | 76.6 |
| MS.gene58273.t1 | AT1G06000 | 28.182 | 220 | 119 | 8 | 3 | 214 | 1 | 189 | 1.32e-15 | 76.3 |
| MS.gene58273.t1 | AT1G51210 | 30.000 | 210 | 124 | 9 | 13 | 207 | 21 | 222 | 6.98e-15 | 73.9 |
| MS.gene58273.t1 | AT5G03490 | 30.345 | 145 | 96 | 4 | 13 | 154 | 20 | 162 | 8.57e-14 | 70.9 |
| MS.gene58273.t1 | AT5G49690 | 28.289 | 152 | 106 | 3 | 3 | 153 | 1 | 150 | 4.47e-12 | 65.9 |
Find 58 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACGAAATGCTCGAGATTT+TGG | 0.125896 | 8.3:+8364614 | MS.gene58273:intron |
| TAACAGATTGGAAGCGCTTT+TGG | 0.258988 | 8.3:+8363851 | MS.gene58273:CDS |
| TCGCAGATTTCTTGTTTCCT+TGG | 0.298202 | 8.3:+8363649 | MS.gene58273:CDS |
| ATACGAAGGTGTTGGTTTAA+AGG | 0.306447 | 8.3:-8363462 | None:intergenic |
| CGCAGATTTCTTGTTTCCTT+GGG | 0.312174 | 8.3:+8363650 | MS.gene58273:CDS |
| CGTCGGGGAGGCCAACTTCT+TGG | 0.312438 | 8.3:-8363506 | None:intergenic |
| TACGAAGGTGTTGGTTTAAA+GGG | 0.314549 | 8.3:-8363461 | None:intergenic |
| CCAACTTCTTGGGAAGAAAA+TGG | 0.316453 | 8.3:-8363495 | None:intergenic |
| CTTCGGCGGAGCTGCATTCA+TGG | 0.322615 | 8.3:-8363820 | None:intergenic |
| ATCACCCATCCAACAGTTCT+TGG | 0.322941 | 8.3:+8363602 | MS.gene58273:CDS |
| GTGTTGATCGATGATCTTAA+AGG | 0.330808 | 8.3:+8364692 | MS.gene58273:CDS |
| GTCGGGGAGGCCAACTTCTT+GGG | 0.401102 | 8.3:-8363505 | None:intergenic |
| GCAATAGAATGAGGAAGATC+AGG | 0.452278 | 8.3:-8363798 | None:intergenic |
| AGGAAGGTGGTTCATCTCAT+AGG | 0.459747 | 8.3:+8364660 | MS.gene58273:CDS |
| TCTGTTTGCTATTTGTGCCA+TGG | 0.461379 | 8.3:+8363728 | MS.gene58273:CDS |
| TTCATCTCATAGGAATTTAA+CGG | 0.462596 | 8.3:+8364670 | MS.gene58273:CDS |
| CTGCTCCAAGAACTGTTGGA+TGG | 0.464929 | 8.3:-8363607 | None:intergenic |
| GGTGCTGCTCCAAGAACTGT+TGG | 0.464955 | 8.3:-8363611 | None:intergenic |
| TCGATGATCTTAAAGGTTTG+AGG | 0.477949 | 8.3:+8364699 | MS.gene58273:CDS |
| TAAGGCTAAACATGCAGCTC+AGG | 0.480096 | 8.3:+8364640 | MS.gene58273:CDS |
| CGATGATCTTAAAGGTTTGA+GGG | 0.489297 | 8.3:+8364700 | MS.gene58273:CDS |
| AAATCTGCGACAATGCAATC+TGG | 0.490988 | 8.3:-8363636 | None:intergenic |
| GAGAGACTTTCTACACCGTC+GGG | 0.498177 | 8.3:-8363522 | None:intergenic |
| AATCTGTTACTCATCTCCTT+CGG | 0.498991 | 8.3:-8363837 | None:intergenic |
| AGAGTCTAGGAATTTGAAGC+TGG | 0.506967 | 8.3:-8363689 | None:intergenic |
| TGCTCCAAGAACTGTTGGAT+GGG | 0.515508 | 8.3:-8363606 | None:intergenic |
| CCTTCCAACGCTAAAATCCT+TGG | 0.517202 | 8.3:+8363429 | MS.gene58273:CDS |
| GAAGGAGATGAGTAACAGAT+TGG | 0.520894 | 8.3:+8363839 | MS.gene58273:CDS |
| CATGAATGCAGCTCCGCCGA+AGG | 0.527728 | 8.3:+8363821 | MS.gene58273:CDS |
| ATATGACCAGGTGATAAGTA+AGG | 0.528714 | 8.3:-8363339 | None:intergenic |
| CGCCACTGACTTCGATAACA+TGG | 0.543159 | 8.3:+8363548 | MS.gene58273:CDS |
| AGAACTGTTGGATGGGTGAT+CGG | 0.543549 | 8.3:-8363599 | None:intergenic |
| GTGTGCTTTAACAGATTCCA+TGG | 0.545350 | 8.3:-8363745 | None:intergenic |
| AAACCGTTGAAAGAGAGTCT+AGG | 0.560071 | 8.3:-8363702 | None:intergenic |
| CAAGAAGTTGGCCTCCCCGA+CGG | 0.561963 | 8.3:+8363507 | MS.gene58273:CDS |
| ATTCCTAGACTCTCTTTCAA+CGG | 0.564591 | 8.3:+8363699 | MS.gene58273:CDS |
| TTCATACCTTACTTATCACC+TGG | 0.565212 | 8.3:+8363333 | MS.gene58273:CDS |
| CATAGAGGTATCATATGACC+AGG | 0.569116 | 8.3:-8363351 | None:intergenic |
| TTAAAGGGATAGATTTGCCA+AGG | 0.573034 | 8.3:-8363446 | None:intergenic |
| GTTGGAAGGTGTGGTTATGA+TGG | 0.573643 | 8.3:-8363415 | None:intergenic |
| GATGGGTGATCGGAGGAGAG+TGG | 0.580506 | 8.3:-8363589 | None:intergenic |
| CAGTGTGAATACGAAGGTGT+TGG | 0.589020 | 8.3:-8363470 | None:intergenic |
| GACCATGTTATCGAAGTCAG+TGG | 0.600006 | 8.3:-8363550 | None:intergenic |
| AGAGAGACTTTCTACACCGT+CGG | 0.605612 | 8.3:-8363523 | None:intergenic |
| GTTCTACAAAATTATTGACA+AGG | 0.607650 | 8.3:-8363899 | None:intergenic |
| GCATTCATGGCAATAGAATG+AGG | 0.608211 | 8.3:-8363807 | None:intergenic |
| CTGTTACTCATCTCCTTCGG+CGG | 0.615393 | 8.3:-8363834 | None:intergenic |
| AAACATGCAGCTCAGGAAGG+TGG | 0.637175 | 8.3:+8364647 | MS.gene58273:CDS |
| AGAGTTGCTATGTCACATAG+AGG | 0.640549 | 8.3:-8363366 | None:intergenic |
| TTTGCAAGTTCATCAACCCA+AGG | 0.643585 | 8.3:-8363666 | None:intergenic |
| ACTGTTGGATGGGTGATCGG+AGG | 0.645895 | 8.3:-8363596 | None:intergenic |
| GCTAAACATGCAGCTCAGGA+AGG | 0.648713 | 8.3:+8364644 | MS.gene58273:CDS |
| GACTTTCTACACCGTCGGGG+AGG | 0.651519 | 8.3:-8363518 | None:intergenic |
| TCTGCGACAATGCAATCTGG+TGG | 0.658763 | 8.3:-8363633 | None:intergenic |
| CATGTTATCGAAGTCAGTGG+CGG | 0.667337 | 8.3:-8363547 | None:intergenic |
| CTGCGACAATGCAATCTGGT+GGG | 0.680202 | 8.3:-8363632 | None:intergenic |
| ATGGAACAGTGTGAATACGA+AGG | 0.698551 | 8.3:-8363476 | None:intergenic |
| AGAGACTTTCTACACCGTCG+GGG | 0.752751 | 8.3:-8363521 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTATCTGAAAAATAACAT+AGG | - | chr8.3:8364124-8364143 | None:intergenic | 20.0% |
| !!! | AATAATTTTGTAGAACTTGA+CGG | + | chr8.3:8363906-8363925 | MS.gene58273:CDS | 20.0% |
| ! | GTTCTACAAAATTATTGACA+AGG | - | chr8.3:8363902-8363921 | None:intergenic | 25.0% |
| ! | TTCATCTCATAGGAATTTAA+CGG | + | chr8.3:8364670-8364689 | MS.gene58273:CDS | 25.0% |
| AAGAAGGGTTTGATTATTAG+GGG | + | chr8.3:8364290-8364309 | MS.gene58273:intron | 30.0% | |
| AGAAGAAGGGTTTGATTATT+AGG | + | chr8.3:8364288-8364307 | MS.gene58273:intron | 30.0% | |
| ATCAGGTTACGAATTTGTAT+GGG | + | chr8.3:8364175-8364194 | MS.gene58273:intron | 30.0% | |
| GAAGAAGGGTTTGATTATTA+GGG | + | chr8.3:8364289-8364308 | MS.gene58273:intron | 30.0% | |
| !! | TTATGATGGTGACATTTTGA+CGG | - | chr8.3:8363404-8363423 | None:intergenic | 30.0% |
| AAAGAAAAATGGCTACCGAA+GGG | + | chr8.3:8364242-8364261 | MS.gene58273:intron | 35.0% | |
| AAGAGTTGTTGCAAAGAAGA+AGG | + | chr8.3:8364274-8364293 | MS.gene58273:intron | 35.0% | |
| AATCTGTTACTCATCTCCTT+CGG | - | chr8.3:8363840-8363859 | None:intergenic | 35.0% | |
| ACTCTTTCTTCAAATCCCTT+CGG | - | chr8.3:8364260-8364279 | None:intergenic | 35.0% | |
| AGAGTTGTTGCAAAGAAGAA+GGG | + | chr8.3:8364275-8364294 | MS.gene58273:intron | 35.0% | |
| ATATGACCAGGTGATAAGTA+AGG | - | chr8.3:8363342-8363361 | None:intergenic | 35.0% | |
| ATTCCTAGACTCTCTTTCAA+CGG | + | chr8.3:8363699-8363718 | MS.gene58273:CDS | 35.0% | |
| CATCAGGTTACGAATTTGTA+TGG | + | chr8.3:8364174-8364193 | MS.gene58273:intron | 35.0% | |
| CGATGATCTTAAAGGTTTGA+GGG | + | chr8.3:8364700-8364719 | MS.gene58273:CDS | 35.0% | |
| GTCAAGAGGAGAAAGAAAAA+TGG | + | chr8.3:8364231-8364250 | MS.gene58273:intron | 35.0% | |
| GTGCATTTATGACACATTGT+GGG | + | chr8.3:8364354-8364373 | MS.gene58273:intron | 35.0% | |
| TAAAATTGTTCTCCATGCAC+TGG | - | chr8.3:8364431-8364450 | None:intergenic | 35.0% | |
| TATTGCAGAGAAAGAGAAGT+TGG | + | chr8.3:8364523-8364542 | MS.gene58273:intron | 35.0% | |
| TCAGTGTTGTACATTTGCTT+TGG | + | chr8.3:8364095-8364114 | MS.gene58273:intron | 35.0% | |
| TCGATGATCTTAAAGGTTTG+AGG | + | chr8.3:8364699-8364718 | MS.gene58273:CDS | 35.0% | |
| TTAAAGGGATAGATTTGCCA+AGG | - | chr8.3:8363449-8363468 | None:intergenic | 35.0% | |
| TTCATACCTTACTTATCACC+TGG | + | chr8.3:8363333-8363352 | MS.gene58273:CDS | 35.0% | |
| ! | GAGACAGTTTTCAAAAGTCA+CGG | + | chr8.3:8363873-8363892 | MS.gene58273:CDS | 35.0% |
| ! | GTGTTGATCGATGATCTTAA+AGG | + | chr8.3:8364692-8364711 | MS.gene58273:CDS | 35.0% |
| !! | ATACGAAGGTGTTGGTTTAA+AGG | - | chr8.3:8363465-8363484 | None:intergenic | 35.0% |
| !! | TACGAAGGTGTTGGTTTAAA+GGG | - | chr8.3:8363464-8363483 | None:intergenic | 35.0% |
| AAACCGTTGAAAGAGAGTCT+AGG | - | chr8.3:8363705-8363724 | None:intergenic | 40.0% | |
| AAATCTGCGACAATGCAATC+TGG | - | chr8.3:8363639-8363658 | None:intergenic | 40.0% | |
| AACTTTATGAGATTGCGTGC+GGG | + | chr8.3:8364144-8364163 | MS.gene58273:intron | 40.0% | |
| AGAGTTGCTATGTCACATAG+AGG | - | chr8.3:8363369-8363388 | None:intergenic | 40.0% | |
| AGGGTTTGATTATTAGGGGT+TGG | + | chr8.3:8364294-8364313 | MS.gene58273:intron | 40.0% | |
| ATGGAACAGTGTGAATACGA+AGG | - | chr8.3:8363479-8363498 | None:intergenic | 40.0% | |
| CAACTTTATGAGATTGCGTG+CGG | + | chr8.3:8364143-8364162 | MS.gene58273:intron | 40.0% | |
| CATTTATGACACATTGTGGG+TGG | + | chr8.3:8364357-8364376 | MS.gene58273:intron | 40.0% | |
| CCAACTTCTTGGGAAGAAAA+TGG | - | chr8.3:8363498-8363517 | None:intergenic | 40.0% | |
| CGAACACTACGAGAAAACTA+CGG | + | chr8.3:8363941-8363960 | MS.gene58273:intron | 40.0% | |
| GAAAGAAAAATGGCTACCGA+AGG | + | chr8.3:8364241-8364260 | MS.gene58273:intron | 40.0% | |
| GAAAGAGAATGAGAGTCAAG+AGG | + | chr8.3:8364217-8364236 | MS.gene58273:intron | 40.0% | |
| GAACACTACGAGAAAACTAC+GGG | + | chr8.3:8363942-8363961 | MS.gene58273:intron | 40.0% | |
| GAAGGAGATGAGTAACAGAT+TGG | + | chr8.3:8363839-8363858 | MS.gene58273:CDS | 40.0% | |
| GAAGGTTGTGAAGAGATTGA+TGG | + | chr8.3:8364565-8364584 | MS.gene58273:intron | 40.0% | |
| GCAATAGAATGAGGAAGATC+AGG | - | chr8.3:8363801-8363820 | None:intergenic | 40.0% | |
| GCATTCATGGCAATAGAATG+AGG | - | chr8.3:8363810-8363829 | None:intergenic | 40.0% | |
| GGGTTTGATTATTAGGGGTT+GGG | + | chr8.3:8364295-8364314 | MS.gene58273:intron | 40.0% | |
| GGTGCATTTATGACACATTG+TGG | + | chr8.3:8364353-8364372 | MS.gene58273:intron | 40.0% | |
| GTGTGCTTTAACAGATTCCA+TGG | - | chr8.3:8363748-8363767 | None:intergenic | 40.0% | |
| GTTCTACGAATAAGAGAAGC+TGG | - | chr8.3:8363990-8364009 | None:intergenic | 40.0% | |
| TTTGCAAGTTCATCAACCCA+AGG | - | chr8.3:8363669-8363688 | None:intergenic | 40.0% | |
| ! | ACGAAATGCTCGAGATTTTG+GGG | + | chr8.3:8364616-8364635 | MS.gene58273:CDS | 40.0% |
| ! | AGACGAAATGCTCGAGATTT+TGG | + | chr8.3:8364614-8364633 | MS.gene58273:intron | 40.0% |
| ! | AGAGTCTAGGAATTTGAAGC+TGG | - | chr8.3:8363692-8363711 | None:intergenic | 40.0% |
| ! | CCATTTTCTTCCCAAGAAGT+TGG | + | chr8.3:8363495-8363514 | MS.gene58273:CDS | 40.0% |
| ! | CGCAGATTTCTTGTTTCCTT+GGG | + | chr8.3:8363650-8363669 | MS.gene58273:CDS | 40.0% |
| ! | GACGAAATGCTCGAGATTTT+GGG | + | chr8.3:8364615-8364634 | MS.gene58273:intron | 40.0% |
| ! | TCGCAGATTTCTTGTTTCCT+TGG | + | chr8.3:8363649-8363668 | MS.gene58273:CDS | 40.0% |
| !! | AGAAGTTGATAACGCAAGTG+CGG | + | chr8.3:8364456-8364475 | MS.gene58273:intron | 40.0% |
| !! | CATAGAGGTATCATATGACC+AGG | - | chr8.3:8363354-8363373 | None:intergenic | 40.0% |
| !! | TCTGTTTGCTATTTGTGCCA+TGG | + | chr8.3:8363728-8363747 | MS.gene58273:CDS | 40.0% |
| !! | TTTGCCAAGGATTTTAGCGT+TGG | - | chr8.3:8363436-8363455 | None:intergenic | 40.0% |
| !!! | TAACAGATTGGAAGCGCTTT+TGG | + | chr8.3:8363851-8363870 | MS.gene58273:CDS | 40.0% |
| ACTTTATGAGATTGCGTGCG+GGG | + | chr8.3:8364145-8364164 | MS.gene58273:intron | 45.0% | |
| AGAACTGTTGGATGGGTGAT+CGG | - | chr8.3:8363602-8363621 | None:intergenic | 45.0% | |
| AGAGAGACTTTCTACACCGT+CGG | - | chr8.3:8363526-8363545 | None:intergenic | 45.0% | |
| AGGAAGGTGGTTCATCTCAT+AGG | + | chr8.3:8364660-8364679 | MS.gene58273:CDS | 45.0% | |
| ATCACCCATCCAACAGTTCT+TGG | + | chr8.3:8363602-8363621 | MS.gene58273:CDS | 45.0% | |
| CATGTTATCGAAGTCAGTGG+CGG | - | chr8.3:8363550-8363569 | None:intergenic | 45.0% | |
| CCTTCCAACGCTAAAATCCT+TGG | + | chr8.3:8363429-8363448 | MS.gene58273:CDS | 45.0% | |
| GACCATGTTATCGAAGTCAG+TGG | - | chr8.3:8363553-8363572 | None:intergenic | 45.0% | |
| GCTCAGAATCATAACCTGAG+GGG | - | chr8.3:8364321-8364340 | None:intergenic | 45.0% | |
| GGCTCAGAATCATAACCTGA+GGG | - | chr8.3:8364322-8364341 | None:intergenic | 45.0% | |
| GGGTTGTTCGTGAGAAGAAA+GGG | + | chr8.3:8364195-8364214 | MS.gene58273:intron | 45.0% | |
| GGTTATGATTCTGAGCCACA+AGG | + | chr8.3:8364325-8364344 | MS.gene58273:intron | 45.0% | |
| GTTCAAGAGAAAGCAGAGAG+AGG | + | chr8.3:8364011-8364030 | MS.gene58273:intron | 45.0% | |
| TAAGGCTAAACATGCAGCTC+AGG | + | chr8.3:8364640-8364659 | MS.gene58273:CDS | 45.0% | |
| TGCTCCAAGAACTGTTGGAT+GGG | - | chr8.3:8363609-8363628 | None:intergenic | 45.0% | |
| TGGCTCAGAATCATAACCTG+AGG | - | chr8.3:8364323-8364342 | None:intergenic | 45.0% | |
| TGGGTTGTTCGTGAGAAGAA+AGG | + | chr8.3:8364194-8364213 | MS.gene58273:intron | 45.0% | |
| ! | CAGAGAAAGAGAAGTTGGTC+GGG | + | chr8.3:8364528-8364547 | MS.gene58273:intron | 45.0% |
| ! | CAGTGTGAATACGAAGGTGT+TGG | - | chr8.3:8363473-8363492 | None:intergenic | 45.0% |
| ! | GCAGAGAAAGAGAAGTTGGT+CGG | + | chr8.3:8364527-8364546 | MS.gene58273:intron | 45.0% |
| ! | GTGAAGAGATTGATGGACAG+TGG | + | chr8.3:8364572-8364591 | MS.gene58273:intron | 45.0% |
| ! | GTTGGAAGGTGTGGTTATGA+TGG | - | chr8.3:8363418-8363437 | None:intergenic | 45.0% |
| !! | AAGTTGATAACGCAAGTGCG+GGG | + | chr8.3:8364458-8364477 | MS.gene58273:intron | 45.0% |
| !! | CCAAGGATTTTAGCGTTGGA+AGG | - | chr8.3:8363432-8363451 | None:intergenic | 45.0% |
| !! | GAAGTTGATAACGCAAGTGC+GGG | + | chr8.3:8364457-8364476 | MS.gene58273:intron | 45.0% |
| !! | GATTTTAGCGTTGGAAGGTG+TGG | - | chr8.3:8363427-8363446 | None:intergenic | 45.0% |
| !! | TGCTCGAGATTTTGGGGATA+AGG | + | chr8.3:8364622-8364641 | MS.gene58273:CDS | 45.0% |
| AAACATGCAGCTCAGGAAGG+TGG | + | chr8.3:8364647-8364666 | MS.gene58273:CDS | 50.0% | |
| AAACTACGGGTCAGAAAGCC+TGG | + | chr8.3:8363955-8363974 | MS.gene58273:intron | 50.0% | |
| AGAGACTTTCTACACCGTCG+GGG | - | chr8.3:8363524-8363543 | None:intergenic | 50.0% | |
| ATTCTGAGCCACAAGGCTGT+GGG | + | chr8.3:8364332-8364351 | MS.gene58273:intron | 50.0% | |
| CTGCGACAATGCAATCTGGT+GGG | - | chr8.3:8363635-8363654 | None:intergenic | 50.0% | |
| CTGCTCCAAGAACTGTTGGA+TGG | - | chr8.3:8363610-8363629 | None:intergenic | 50.0% | |
| CTGTTACTCATCTCCTTCGG+CGG | - | chr8.3:8363837-8363856 | None:intergenic | 50.0% | |
| GAGAGACTTTCTACACCGTC+GGG | - | chr8.3:8363525-8363544 | None:intergenic | 50.0% | |
| GCAGTAGAGTGGTGCTCATT+TGG | + | chr8.3:8364500-8364519 | MS.gene58273:intron | 50.0% | |
| GCTAAACATGCAGCTCAGGA+AGG | + | chr8.3:8364644-8364663 | MS.gene58273:CDS | 50.0% | |
| GTGTGCATGAGTGTCTGAGT+TGG | + | chr8.3:8364051-8364070 | MS.gene58273:intron | 50.0% | |
| TAAATGCACCCACAGCCTTG+TGG | - | chr8.3:8364343-8364362 | None:intergenic | 50.0% | |
| TCTGCGACAATGCAATCTGG+TGG | - | chr8.3:8363636-8363655 | None:intergenic | 50.0% | |
| TGGAAATGGGTGCAGTAGAG+TGG | + | chr8.3:8364489-8364508 | MS.gene58273:intron | 50.0% | |
| TTGTGGGTGGAATTCCATCG+TGG | + | chr8.3:8364370-8364389 | MS.gene58273:intron | 50.0% | |
| ! | AGAAAGAGAAGTTGGTCGGG+AGG | + | chr8.3:8364531-8364550 | MS.gene58273:intron | 50.0% |
| ! | CGGGAGGGATAGTATTGAGA+AGG | + | chr8.3:8364547-8364566 | MS.gene58273:intron | 50.0% |
| ! | GAAAGAGAAGTTGGTCGGGA+GGG | + | chr8.3:8364532-8364551 | MS.gene58273:intron | 50.0% |
| !! | AGTTGATAACGCAAGTGCGG+GGG | + | chr8.3:8364459-8364478 | MS.gene58273:intron | 50.0% |
| !! | CGCCACTGACTTCGATAACA+TGG | + | chr8.3:8363548-8363567 | MS.gene58273:CDS | 50.0% |
| !! | GGTGACATTTTGACGGCGTA+AGG | - | chr8.3:8363397-8363416 | None:intergenic | 50.0% |
| ACTGTTGGATGGGTGATCGG+AGG | - | chr8.3:8363599-8363618 | None:intergenic | 55.0% | |
| AGAAGCTGGACCAAGATGCC+AGG | - | chr8.3:8363976-8363995 | None:intergenic | 55.0% | |
| ATAACGCAAGTGCGGGGGAT+TGG | + | chr8.3:8364464-8364483 | MS.gene58273:intron | 55.0% | |
| ATGATCACGTGGCCAGTGCA+TGG | + | chr8.3:8364416-8364435 | MS.gene58273:intron | 55.0% | |
| CAGGGGTTCCAATGATCACG+TGG | + | chr8.3:8364405-8364424 | MS.gene58273:intron | 55.0% | |
| CGGGGGATTGGGATGGAAAT+GGG | + | chr8.3:8364476-8364495 | MS.gene58273:intron | 55.0% | |
| GATTCTGAGCCACAAGGCTG+TGG | + | chr8.3:8364331-8364350 | MS.gene58273:intron | 55.0% | |
| GGTCAGAAAGCCTGGCATCT+TGG | + | chr8.3:8363963-8363982 | MS.gene58273:intron | 55.0% | |
| GTGGAATTCCATCGTGGAGG+CGG | + | chr8.3:8364376-8364395 | MS.gene58273:intron | 55.0% | |
| TAACGCAAGTGCGGGGGATT+GGG | + | chr8.3:8364465-8364484 | MS.gene58273:intron | 55.0% | |
| TGGGTGGAATTCCATCGTGG+AGG | + | chr8.3:8364373-8364392 | MS.gene58273:intron | 55.0% | |
| ! | TGCACTGGCCACGTGATCAT+TGG | - | chr8.3:8364416-8364435 | None:intergenic | 55.0% |
| !! | GGTGCTGCTCCAAGAACTGT+TGG | - | chr8.3:8363614-8363633 | None:intergenic | 55.0% |
| ATCGTGGAGGCGGTTAGTGC+AGG | + | chr8.3:8364386-8364405 | MS.gene58273:intron | 60.0% | |
| CATGAATGCAGCTCCGCCGA+AGG | + | chr8.3:8363821-8363840 | MS.gene58273:CDS | 60.0% | |
| CTTCGGCGGAGCTGCATTCA+TGG | - | chr8.3:8363823-8363842 | None:intergenic | 60.0% | |
| GACTTTCTACACCGTCGGGG+AGG | - | chr8.3:8363521-8363540 | None:intergenic | 60.0% | |
| GATGGGTGATCGGAGGAGAG+TGG | - | chr8.3:8363592-8363611 | None:intergenic | 60.0% | |
| GCGGGGGATTGGGATGGAAA+TGG | + | chr8.3:8364475-8364494 | MS.gene58273:intron | 60.0% | |
| GTCGGGGAGGCCAACTTCTT+GGG | - | chr8.3:8363508-8363527 | None:intergenic | 60.0% | |
| TATTAGGGGTTGGGCCCCTC+AGG | + | chr8.3:8364304-8364323 | MS.gene58273:intron | 60.0% | |
| TCGTGGAGGCGGTTAGTGCA+GGG | + | chr8.3:8364387-8364406 | MS.gene58273:intron | 60.0% | |
| TGCACTAACCGCCTCCACGA+TGG | - | chr8.3:8364387-8364406 | None:intergenic | 60.0% | |
| ! | CAAGAAGTTGGCCTCCCCGA+CGG | + | chr8.3:8363507-8363526 | MS.gene58273:CDS | 60.0% |
| CGTCGGGGAGGCCAACTTCT+TGG | - | chr8.3:8363509-8363528 | None:intergenic | 65.0% | |
| CGTGGAGGCGGTTAGTGCAG+GGG | + | chr8.3:8364388-8364407 | MS.gene58273:intron | 65.0% | |
| GCAAGTGCGGGGGATTGGGA+TGG | + | chr8.3:8364469-8364488 | MS.gene58273:intron | 65.0% | |
| !! | GCGTGCGGGGTAGAAGCATC+AGG | + | chr8.3:8364158-8364177 | MS.gene58273:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 8363291 | 8364730 | 8363291 | ID=MS.gene58273 |
| chr8.3 | mRNA | 8363291 | 8364730 | 8363291 | ID=MS.gene58273.t1;Parent=MS.gene58273 |
| chr8.3 | exon | 8363291 | 8363934 | 8363291 | ID=MS.gene58273.t1.exon1;Parent=MS.gene58273.t1 |
| chr8.3 | CDS | 8363291 | 8363934 | 8363291 | ID=cds.MS.gene58273.t1;Parent=MS.gene58273.t1 |
| chr8.3 | exon | 8364616 | 8364730 | 8364616 | ID=MS.gene58273.t1.exon2;Parent=MS.gene58273.t1 |
| chr8.3 | CDS | 8364616 | 8364730 | 8364616 | ID=cds.MS.gene58273.t1;Parent=MS.gene58273.t1 |
| Gene Sequence |
| Protein sequence |
