Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58432.t1 | RHN63663.1 | 100 | 56 | 0 | 0 | 21 | 76 | 22 | 77 | 7.80E-26 | 125.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58432.t1 | Q2Q493 | 61.6 | 73 | 26 | 1 | 1 | 71 | 1 | 73 | 5.1e-18 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58432.t1 | A0A396IG90 | 100.0 | 56 | 0 | 0 | 21 | 76 | 22 | 77 | 5.6e-26 | 125.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene58432.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049669 | MS.gene58432 | 0.805118 | 1.56E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene58432 | 0.806062 | 9.87E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene58432 | 0.813444 | 2.55E-51 | -1.69E-46 |
| MS.gene050680 | MS.gene58432 | 0.801059 | 1.08E-48 | -1.69E-46 |
| MS.gene050750 | MS.gene58432 | 0.805346 | 1.40E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene58432 | 0.81013 | 1.34E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene58432 | 0.806764 | 7.02E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene58432 | 0.802776 | 4.78E-49 | -1.69E-46 |
| MS.gene054405 | MS.gene58432 | 0.804413 | 2.19E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene58432 | 0.806679 | 7.32E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene58432 | 0.806178 | 9.33E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene58432 | 0.80117 | 1.02E-48 | -1.69E-46 |
| MS.gene057060 | MS.gene58432 | 0.809266 | 2.06E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene58432 | 0.802366 | 5.81E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene58432 | 0.80107 | 1.07E-48 | -1.69E-46 |
| MS.gene059161 | MS.gene58432 | 0.808695 | 2.73E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene58432 | 0.800695 | 1.28E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58432.t1 | MTR_3g039810 | 67.568 | 74 | 17 | 3 | 1 | 71 | 1 | 70 | 6.29e-27 | 94.0 |
| MS.gene58432.t1 | MTR_6g012220 | 68.116 | 69 | 18 | 2 | 1 | 69 | 1 | 65 | 8.26e-26 | 90.9 |
| MS.gene58432.t1 | MTR_3g100650 | 62.963 | 54 | 17 | 1 | 21 | 71 | 77 | 130 | 2.09e-17 | 73.9 |
| MS.gene58432.t1 | MTR_8g467340 | 57.627 | 59 | 20 | 1 | 17 | 70 | 57 | 115 | 8.52e-16 | 70.1 |
| MS.gene58432.t1 | MTR_5g065440 | 50.000 | 78 | 20 | 2 | 13 | 71 | 31 | 108 | 1.35e-15 | 68.9 |
| MS.gene58432.t1 | MTR_1g077020 | 59.649 | 57 | 18 | 2 | 19 | 71 | 4 | 59 | 1.57e-15 | 67.4 |
| MS.gene58432.t1 | MTR_7g010300 | 56.364 | 55 | 19 | 1 | 21 | 70 | 85 | 139 | 2.04e-15 | 68.9 |
| MS.gene58432.t1 | MTR_2g076780 | 58.000 | 50 | 18 | 1 | 24 | 70 | 37 | 86 | 1.70e-14 | 65.5 |
| MS.gene58432.t1 | MTR_4g134580 | 59.649 | 57 | 18 | 2 | 24 | 76 | 113 | 168 | 3.89e-14 | 65.5 |
| MS.gene58432.t1 | MTR_5g020510 | 52.459 | 61 | 24 | 2 | 17 | 73 | 92 | 151 | 8.42e-14 | 64.3 |
| MS.gene58432.t1 | MTR_8g069080 | 56.000 | 50 | 19 | 1 | 24 | 70 | 69 | 118 | 1.28e-13 | 63.5 |
| MS.gene58432.t1 | MTR_5g032600 | 58.000 | 50 | 18 | 1 | 24 | 70 | 78 | 127 | 1.41e-13 | 63.5 |
| MS.gene58432.t1 | MTR_6g012280 | 59.615 | 52 | 16 | 2 | 24 | 70 | 91 | 142 | 1.76e-13 | 63.5 |
| MS.gene58432.t1 | MTR_6g073013 | 56.000 | 50 | 18 | 1 | 24 | 69 | 19 | 68 | 1.83e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58432.t1 | AT1G18835 | 64.384 | 73 | 24 | 1 | 1 | 71 | 1 | 73 | 1.56e-26 | 92.8 |
| MS.gene58432.t1 | AT3G28917 | 57.143 | 84 | 23 | 2 | 1 | 71 | 1 | 84 | 3.90e-24 | 87.4 |
| MS.gene58432.t1 | AT1G74660 | 67.273 | 55 | 15 | 1 | 20 | 71 | 35 | 89 | 2.48e-19 | 75.1 |
| MS.gene58432.t1 | AT1G74660 | 67.273 | 55 | 15 | 1 | 20 | 71 | 35 | 89 | 3.49e-19 | 74.7 |
| MS.gene58432.t1 | AT3G50890 | 51.389 | 72 | 28 | 3 | 6 | 71 | 40 | 110 | 4.04e-16 | 70.1 |
| MS.gene58432.t1 | AT2G18350 | 54.237 | 59 | 24 | 1 | 16 | 71 | 74 | 132 | 6.87e-16 | 69.7 |
| MS.gene58432.t1 | AT1G14440 | 61.404 | 57 | 17 | 2 | 19 | 71 | 85 | 140 | 8.62e-16 | 69.7 |
| MS.gene58432.t1 | AT1G14440 | 61.404 | 57 | 17 | 2 | 19 | 71 | 85 | 140 | 8.62e-16 | 69.7 |
| MS.gene58432.t1 | AT5G15210 | 59.615 | 52 | 16 | 1 | 24 | 70 | 56 | 107 | 3.57e-15 | 67.8 |
| MS.gene58432.t1 | AT4G24660 | 61.111 | 54 | 16 | 2 | 22 | 71 | 47 | 99 | 5.85e-15 | 66.6 |
| MS.gene58432.t1 | AT2G02540 | 62.264 | 53 | 15 | 2 | 22 | 70 | 85 | 136 | 6.79e-15 | 67.4 |
| MS.gene58432.t1 | AT2G02540 | 62.264 | 53 | 15 | 2 | 22 | 70 | 85 | 136 | 6.79e-15 | 67.4 |
| MS.gene58432.t1 | AT4G24660 | 61.111 | 54 | 16 | 2 | 22 | 71 | 47 | 99 | 8.49e-15 | 66.2 |
| MS.gene58432.t1 | AT5G42780 | 60.000 | 50 | 19 | 1 | 24 | 72 | 64 | 113 | 8.54e-15 | 66.6 |
| MS.gene58432.t1 | AT1G75240 | 55.932 | 59 | 23 | 1 | 16 | 71 | 68 | 126 | 1.21e-14 | 66.6 |
| MS.gene58432.t1 | AT5G39760 | 50.000 | 66 | 28 | 1 | 9 | 69 | 41 | 106 | 2.06e-14 | 66.2 |
| MS.gene58432.t1 | AT1G69600 | 53.333 | 60 | 23 | 1 | 15 | 69 | 22 | 81 | 2.27e-14 | 65.5 |
| MS.gene58432.t1 | AT3G28920 | 55.932 | 59 | 21 | 1 | 22 | 75 | 50 | 108 | 2.85e-14 | 65.9 |
| MS.gene58432.t1 | AT5G65410 | 56.863 | 51 | 19 | 1 | 24 | 71 | 75 | 125 | 1.06e-13 | 63.9 |
| MS.gene58432.t1 | AT5G60480 | 52.830 | 53 | 20 | 1 | 22 | 69 | 35 | 87 | 1.92e-13 | 62.8 |
| MS.gene58432.t1 | AT1G14687 | 49.020 | 51 | 24 | 1 | 19 | 69 | 2 | 50 | 5.11e-11 | 55.5 |
Find 13 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAATTCATGGCATGTTC+AGG | 0.335777 | 8.3:+15992674 | MS.gene58432:CDS |
| GTGCTGCTTGCGGTTGTCAT+AGG | 0.419461 | 8.3:+15992714 | MS.gene58432:CDS |
| GTCATAGGAACTTCCACAAA+AGG | 0.480871 | 8.3:+15992729 | MS.gene58432:CDS |
| CTATGCTGTTGATGGATGTA+TGG | 0.485307 | 8.3:+15992655 | MS.gene58432:CDS |
| AAGAATCATGCAGCATATAG+TGG | 0.485623 | 8.3:+15992629 | MS.gene58432:CDS |
| AGTGGAGGCTATGCTGTTGA+TGG | 0.489516 | 8.3:+15992647 | MS.gene58432:CDS |
| TGATGGATGTATGGAATTCA+TGG | 0.495255 | 8.3:+15992664 | MS.gene58432:CDS |
| TGGAATTCATGGCATGTTCA+GGG | 0.512543 | 8.3:+15992675 | MS.gene58432:CDS |
| AAAACGGCAAGTGGTGGTGG+TGG | 0.605250 | 8.3:+15992550 | MS.gene58432:CDS |
| GCACTTACATGTGCTGCTTG+CGG | 0.620281 | 8.3:+15992704 | MS.gene58432:CDS |
| GGAATTCATGGCATGTTCAG+GGG | 0.647724 | 8.3:+15992676 | MS.gene58432:CDS |
| AATCATGCAGCATATAGTGG+AGG | 0.667408 | 8.3:+15992632 | MS.gene58432:CDS |
| GTATTGCAAAGTACAGACTG+AGG | 0.757439 | 8.3:+15992755 | MS.gene58432:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCAAAAAATACTGTTGGATA+TGG | + | chr8.3:15992596-15992615 | MS.gene58432:CDS | 25.0% |
| ! | TCATCTTCAAAAAATACTGT+TGG | + | chr8.3:15992590-15992609 | MS.gene58432:CDS | 25.0% |
| ! | TTTGCAATACTTTCCTTTTG+TGG | - | chr8.3:15992745-15992764 | None:intergenic | 30.0% |
| AAGAATCATGCAGCATATAG+TGG | + | chr8.3:15992629-15992648 | MS.gene58432:CDS | 35.0% | |
| ! | TGATGGATGTATGGAATTCA+TGG | + | chr8.3:15992664-15992683 | MS.gene58432:CDS | 35.0% |
| AATCATGCAGCATATAGTGG+AGG | + | chr8.3:15992632-15992651 | MS.gene58432:CDS | 40.0% | |
| ATGGAATTCATGGCATGTTC+AGG | + | chr8.3:15992674-15992693 | MS.gene58432:CDS | 40.0% | |
| GTCATAGGAACTTCCACAAA+AGG | + | chr8.3:15992729-15992748 | MS.gene58432:CDS | 40.0% | |
| TGGAATTCATGGCATGTTCA+GGG | + | chr8.3:15992675-15992694 | MS.gene58432:CDS | 40.0% | |
| ! | CTATGCTGTTGATGGATGTA+TGG | + | chr8.3:15992655-15992674 | MS.gene58432:CDS | 40.0% |
| GGAATTCATGGCATGTTCAG+GGG | + | chr8.3:15992676-15992695 | MS.gene58432:CDS | 45.0% | |
| AGTGGAGGCTATGCTGTTGA+TGG | + | chr8.3:15992647-15992666 | MS.gene58432:CDS | 50.0% | |
| GAAAAAACGGCAAGTGGTGG+TGG | + | chr8.3:15992547-15992566 | MS.gene58432:CDS | 50.0% | |
| !! | GCACTTACATGTGCTGCTTG+CGG | + | chr8.3:15992704-15992723 | MS.gene58432:CDS | 50.0% |
| !! | AAAACGGCAAGTGGTGGTGG+TGG | + | chr8.3:15992550-15992569 | MS.gene58432:CDS | 55.0% |
| !! | GTGCTGCTTGCGGTTGTCAT+AGG | + | chr8.3:15992714-15992733 | MS.gene58432:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 15992545 | 15992775 | 15992545 | ID=MS.gene58432 |
| chr8.3 | mRNA | 15992545 | 15992775 | 15992545 | ID=MS.gene58432.t1;Parent=MS.gene58432 |
| chr8.3 | exon | 15992545 | 15992775 | 15992545 | ID=MS.gene58432.t1.exon1;Parent=MS.gene58432.t1 |
| chr8.3 | CDS | 15992545 | 15992775 | 15992545 | ID=cds.MS.gene58432.t1;Parent=MS.gene58432.t1 |
| Gene Sequence |
| Protein sequence |
