AlfalfaGEDB | MS.gene58432

M. sativa cultivar XinJiangDaYe / MS.gene58432

KEGG: -
GO: -
PFAM: PF04770.15

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58432.t1	RHN63663.1	100	56	0	0	21	76	22	77	7.80E-26	125.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58432.t1	Q2Q493	61.6	73	26	1	1	71	1	73	5.1e-18	91.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58432.t1	A0A396IG90	100.0	56	0	0	21	76	22	77	5.6e-26	125.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene58432.t1	TF	zf-HD

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049669	MS.gene58432	0.805118	1.56E-49	-1.69E-46
MS.gene050020	MS.gene58432	0.806062	9.87E-50	-1.69E-46
MS.gene050442	MS.gene58432	0.813444	2.55E-51	-1.69E-46
MS.gene050680	MS.gene58432	0.801059	1.08E-48	-1.69E-46
MS.gene050750	MS.gene58432	0.805346	1.40E-49	-1.69E-46
MS.gene050807	MS.gene58432	0.81013	1.34E-50	-1.69E-46
MS.gene050953	MS.gene58432	0.806764	7.02E-50	-1.69E-46
MS.gene053166	MS.gene58432	0.802776	4.78E-49	-1.69E-46
MS.gene054405	MS.gene58432	0.804413	2.19E-49	-1.69E-46
MS.gene055701	MS.gene58432	0.806679	7.32E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58432.t1	MTR_3g039810	67.568	74	17	3	1	71	1	70	6.29e-27	94.0
MS.gene58432.t1	MTR_6g012220	68.116	69	18	2	1	69	1	65	8.26e-26	90.9
MS.gene58432.t1	MTR_3g100650	62.963	54	17	1	21	71	77	130	2.09e-17	73.9
MS.gene58432.t1	MTR_8g467340	57.627	59	20	1	17	70	57	115	8.52e-16	70.1
MS.gene58432.t1	MTR_5g065440	50.000	78	20	2	13	71	31	108	1.35e-15	68.9
MS.gene58432.t1	MTR_1g077020	59.649	57	18	2	19	71	4	59	1.57e-15	67.4
MS.gene58432.t1	MTR_7g010300	56.364	55	19	1	21	70	85	139	2.04e-15	68.9
MS.gene58432.t1	MTR_2g076780	58.000	50	18	1	24	70	37	86	1.70e-14	65.5
MS.gene58432.t1	MTR_4g134580	59.649	57	18	2	24	76	113	168	3.89e-14	65.5
MS.gene58432.t1	MTR_5g020510	52.459	61	24	2	17	73	92	151	8.42e-14	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58432.t1	AT1G18835	64.384	73	24	1	1	71	1	73	1.56e-26	92.8
MS.gene58432.t1	AT3G28917	57.143	84	23	2	1	71	1	84	3.90e-24	87.4
MS.gene58432.t1	AT1G74660	67.273	55	15	1	20	71	35	89	2.48e-19	75.1
MS.gene58432.t1	AT1G74660	67.273	55	15	1	20	71	35	89	3.49e-19	74.7
MS.gene58432.t1	AT3G50890	51.389	72	28	3	6	71	40	110	4.04e-16	70.1
MS.gene58432.t1	AT2G18350	54.237	59	24	1	16	71	74	132	6.87e-16	69.7
MS.gene58432.t1	AT1G14440	61.404	57	17	2	19	71	85	140	8.62e-16	69.7
MS.gene58432.t1	AT1G14440	61.404	57	17	2	19	71	85	140	8.62e-16	69.7
MS.gene58432.t1	AT5G15210	59.615	52	16	1	24	70	56	107	3.57e-15	67.8
MS.gene58432.t1	AT4G24660	61.111	54	16	2	22	71	47	99	5.85e-15	66.6

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Find 13 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGGCAAGTGGTGGTGG+TGG	0.605250	8.3:+15992550	MS.gene58432:CDS
AAGAATCATGCAGCATATAG+TGG	0.485623	8.3:+15992629	MS.gene58432:CDS
AATCATGCAGCATATAGTGG+AGG	0.667408	8.3:+15992632	MS.gene58432:CDS
AGTGGAGGCTATGCTGTTGA+TGG	0.489516	8.3:+15992647	MS.gene58432:CDS
ATGGAATTCATGGCATGTTC+AGG	0.335777	8.3:+15992674	MS.gene58432:CDS
CTATGCTGTTGATGGATGTA+TGG	0.485307	8.3:+15992655	MS.gene58432:CDS
GCACTTACATGTGCTGCTTG+CGG	0.620281	8.3:+15992704	MS.gene58432:CDS
GGAATTCATGGCATGTTCAG+GGG	0.647724	8.3:+15992676	MS.gene58432:CDS
GTATTGCAAAGTACAGACTG+AGG	0.757439	8.3:+15992755	MS.gene58432:CDS
GTCATAGGAACTTCCACAAA+AGG	0.480871	8.3:+15992729	MS.gene58432:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAATCATGCAGCATATAG+TGG	+	chr8.3:15992629-15992648	MS.gene58432:CDS	35.0%
	AATCATGCAGCATATAGTGG+AGG	+	chr8.3:15992632-15992651	MS.gene58432:CDS	40.0%
	ATGGAATTCATGGCATGTTC+AGG	+	chr8.3:15992674-15992693	MS.gene58432:CDS	40.0%
	GTCATAGGAACTTCCACAAA+AGG	+	chr8.3:15992729-15992748	MS.gene58432:CDS	40.0%
	TGGAATTCATGGCATGTTCA+GGG	+	chr8.3:15992675-15992694	MS.gene58432:CDS	40.0%
	GGAATTCATGGCATGTTCAG+GGG	+	chr8.3:15992676-15992695	MS.gene58432:CDS	45.0%
	AGTGGAGGCTATGCTGTTGA+TGG	+	chr8.3:15992647-15992666	MS.gene58432:CDS	50.0%
	GAAAAAACGGCAAGTGGTGG+TGG	+	chr8.3:15992547-15992566	MS.gene58432:CDS	50.0%
!	TCAAAAAATACTGTTGGATA+TGG	+	chr8.3:15992596-15992615	MS.gene58432:CDS	25.0%
!	TCATCTTCAAAAAATACTGT+TGG	+	chr8.3:15992590-15992609	MS.gene58432:CDS	25.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	15992545	15992775	15992545	ID=MS.gene58432
chr8.3	mRNA	15992545	15992775	15992545	ID=MS.gene58432.t1;Parent=MS.gene58432
chr8.3	exon	15992545	15992775	15992545	ID=MS.gene58432.t1.exon1;Parent=MS.gene58432.t1
chr8.3	CDS	15992545	15992775	15992545	ID=cds.MS.gene58432.t1;Parent=MS.gene58432.t1

Gene Sequence	>MS.gene58432 ATGAAAAAACGGCAAGTGGTGGTGGTGGTGAAACGCAACAGTACTTCATCTTCAAAAAATACTGTTGGATATGGAGAGTGTCAAAAGAATCATGCAGCATATAGTGGAGGCTATGCTGTTGATGGATGTATGGAATTCATGGCATGTTCAGGGGAAGAAGCACTTACATGTGCTGCTTGCGGTTGTCATAGGAACTTCCACAAAAGGAAAGTATTGCAAAGTACAGACTGA
Protein sequence	>MS.gene58432.t1 MKKRQVVVVVKRNSTSSSKNTVGYGECQKNHAAYSGGYAVDGCMEFMACSGEEALTCAACGCHRNFHKRKVLQSTD