AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58600.t1 MTR_5g035980 96.018 452 13 4 1 451 1 448 0.0 871
MS.gene58600.t1 MTR_3g078380 52.558 430 149 14 37 451 25 414 1.05e-135 397
MS.gene58600.t1 MTR_4g106480 50.711 422 164 9 35 451 19 401 1.47e-133 390
MS.gene58600.t1 MTR_8g089650 47.242 417 157 13 45 448 64 430 2.28e-110 333
MS.gene58600.t1 MTR_4g104410 42.967 391 165 15 61 446 26 363 5.54e-82 257
MS.gene58600.t1 MTR_5g018520 34.554 437 227 10 13 447 41 420 4.84e-78 250
MS.gene58600.t1 MTR_1g021590 36.000 400 202 9 48 446 85 431 6.08e-77 248
MS.gene58600.t1 MTR_4g068457 38.032 376 180 10 74 446 131 456 4.99e-71 233
MS.gene58600.t1 MTR_2g060070 34.103 390 195 13 59 446 52 381 1.42e-64 214
MS.gene58600.t1 MTR_3g110185 32.962 449 226 12 18 448 20 411 8.47e-52 187
MS.gene58600.t1 MTR_2g101410 32.000 400 202 14 58 448 71 409 3.91e-47 168
MS.gene58600.t1 MTR_2g086140 32.934 167 101 4 246 409 16 174 5.14e-14 75.1
MS.gene58600.t1 MTR_4g053675 31.361 169 105 4 246 409 16 178 2.67e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58600.t1 AT1G24130 52.222 450 171 10 7 451 5 415 1.33e-140 409
MS.gene58600.t1 AT5G50120 46.117 412 183 10 43 449 10 387 4.88e-118 350
MS.gene58600.t1 AT1G24530 45.874 412 163 13 39 448 52 405 4.16e-103 313
MS.gene58600.t1 AT2G26490 37.037 378 185 8 74 451 99 423 2.76e-75 243
MS.gene58600.t1 AT3G50390 35.323 402 198 11 53 446 77 424 8.09e-72 234
MS.gene58600.t1 AT3G18950 35.325 385 192 10 66 446 133 464 1.61e-68 226
MS.gene58600.t1 AT3G51930 34.951 412 221 12 38 446 27 394 4.25e-68 223
MS.gene58600.t1 AT4G34380 35.279 377 192 9 74 446 128 456 1.87e-67 224
MS.gene58600.t1 AT1G49450 35.567 388 193 10 66 446 129 466 3.86e-67 222
MS.gene58600.t1 AT1G47610 33.854 384 197 13 68 449 21 349 1.48e-61 204
MS.gene58600.t1 AT1G21650 28.191 188 104 6 198 383 522 680 1.18e-11 67.8
MS.gene58600.t1 AT1G21651 27.660 188 105 6 198 383 522 680 1.22e-11 67.4
MS.gene58600.t1 AT3G44530 30.909 165 101 5 246 406 16 171 2.83e-11 66.2
MS.gene58600.t1 AT3G44530 30.909 165 101 5 246 406 16 171 2.96e-11 66.2
MS.gene58600.t1 AT5G16750 25.296 253 149 11 166 405 361 586 6.15e-11 65.1
MS.gene58600.t1 AT4G02730 24.528 265 142 9 174 405 64 303 9.64e-11 63.5

Find 57 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTGTTGTTTCATCTTC+TGG 0.080562 5.3:+24561073 MS.gene58600:CDS
TAGCCTTAGTATGCCCTCTT+AGG 0.234642 5.3:-24561390 None:intergenic
CTGATTATAGTGTTAGATTA+TGG 0.250597 5.3:+24561458 MS.gene58600:CDS
AACAACAATCATTGACTTGA+TGG 0.262801 5.3:-24560696 None:intergenic
CACTCCAAGTTTCTCTTTAG+TGG 0.274749 5.3:+24560569 MS.gene58600:CDS
CAATTGTTGTTTCATCTTCT+GGG 0.279540 5.3:+24561074 MS.gene58600:CDS
AAATCTTCTTATTTCACTAT+TGG 0.282755 5.3:-24560597 None:intergenic
CTGATAGAAAGATCAAAATT+TGG 0.302795 5.3:+24561116 MS.gene58600:CDS
TCTTCTGGGGTTGTTTATAC+AGG 0.311488 5.3:+24561088 MS.gene58600:CDS
GGGATAGAACATTCAAAATA+TGG 0.313540 5.3:+24560990 MS.gene58600:CDS
TACTAAGGCTATATTATGTT+TGG 0.339007 5.3:+24561402 MS.gene58600:CDS
TCTCTTGCTGTTTCGAAAGA+TGG 0.341375 5.3:+24560944 MS.gene58600:CDS
AGCATTATTCGAAATTGTTC+CGG 0.359690 5.3:+24561322 MS.gene58600:CDS
CTCATCAAGATCACAAAATC+CGG 0.361786 5.3:+24560738 MS.gene58600:CDS
TTGATTGTGATATCAAAGTT+TGG 0.373353 5.3:+24561641 MS.gene58600:CDS
AGACCCCGACGACGAAAATA+TGG 0.377517 5.3:+24561354 MS.gene58600:CDS
TAGAAAAGCTGTTAAGTGTT+TGG 0.380566 5.3:+24561534 MS.gene58600:CDS
TTTGGTTGTGATGGATGATT+TGG 0.392229 5.3:+24561420 MS.gene58600:CDS
AAGATCACAAAATCCGGGTT+TGG 0.420178 5.3:+24560744 MS.gene58600:CDS
TTTGAAGCTTGAAGAGCAAA+AGG 0.427788 5.3:-24560629 None:intergenic
AGATGGATTGTTTCTTTACT+CGG 0.453556 5.3:+24560961 MS.gene58600:CDS
AGTGTTAGATTATGGAAAAG+AGG 0.466262 5.3:+24561466 MS.gene58600:CDS
ATTCTGAATGAATGTGATCA+TGG 0.475540 5.3:-24560409 None:intergenic
CTATGCATTTAAATAGCCTC+TGG 0.479262 5.3:-24560808 None:intergenic
GTCCTAGTTGGTGCCCTAAG+AGG 0.490038 5.3:+24561376 MS.gene58600:CDS
TGTTTCTTTACTCGGCTTCG+TGG 0.493077 5.3:+24560969 MS.gene58600:CDS
GTGTAGAGCAAGGGATGAGA+TGG 0.499982 5.3:-24560549 None:intergenic
GTCGGGGTCTTGGTCTAGTC+CGG 0.501507 5.3:-24561341 None:intergenic
TATATTATGTTTGGTTGTGA+TGG 0.503707 5.3:+24561411 MS.gene58600:CDS
TGTAGAGCAAGGGATGAGAT+GGG 0.504036 5.3:-24560548 None:intergenic
GAAGGAACAGAAGGTAGACT+TGG 0.519410 5.3:-24560461 None:intergenic
AGCCTTAGTATGCCCTCTTA+GGG 0.526558 5.3:-24561389 None:intergenic
GTTTCTTTACTCGGCTTCGT+GGG 0.536613 5.3:+24560970 MS.gene58600:CDS
GCTTTAGCACTAAACAAAGA+TGG 0.541869 5.3:+24561241 MS.gene58600:CDS
AGAACCACTAAAGAGAAACT+TGG 0.542022 5.3:-24560573 None:intergenic
GAAGTGAGAGAAGGAACAGA+AGG 0.544583 5.3:-24560470 None:intergenic
ATGGATGATTTGGTGTGTAG+TGG 0.553153 5.3:+24561430 MS.gene58600:CDS
TCCTAGTTGGTGCCCTAAGA+GGG 0.557068 5.3:+24561377 MS.gene58600:CDS
ATAGCAGATGATTCCAAAAG+TGG 0.557521 5.3:+24561559 MS.gene58600:CDS
TTGAAGCTTGAAGAGCAAAA+GGG 0.560630 5.3:-24560628 None:intergenic
TGCCCTAAGAGGGCATACTA+AGG 0.569818 5.3:+24561387 MS.gene58600:CDS
GACGAAAATATGGTCCTAGT+TGG 0.570714 5.3:+24561364 MS.gene58600:CDS
TTCAAACAATATCAACAACT+TGG 0.579730 5.3:+24560646 MS.gene58600:CDS
GCCCTCTTAGGGCACCAACT+AGG 0.579791 5.3:-24561378 None:intergenic
AATTGTTGTTTCATCTTCTG+GGG 0.581898 5.3:+24561075 MS.gene58600:CDS
AACTTGGTAGCAATCAGTAA+TGG 0.589681 5.3:+24560662 MS.gene58600:CDS
GAAACTTGGAGTGTAGAGCA+AGG 0.590373 5.3:-24560559 None:intergenic
AAACTTGGAGTGTAGAGCAA+GGG 0.599872 5.3:-24560558 None:intergenic
AGTTCTTATCTTGTTTACAG+TGG 0.604622 5.3:+24561613 MS.gene58600:CDS
TCATCAAGATCACAAAATCC+GGG 0.616047 5.3:+24560739 MS.gene58600:CDS
GGTGGGGTTGATGATGATGA+TGG 0.618938 5.3:+24561589 MS.gene58600:CDS
TTCTGAATGAATGTGATCAT+GGG 0.626189 5.3:-24560408 None:intergenic
GATTGTTGTGAAGTGAGAGA+AGG 0.627747 5.3:-24560479 None:intergenic
GGAGACATAAGAAGTATACA+TGG 0.631337 5.3:+24560897 MS.gene58600:CDS
GAGACATAAGAAGTATACAT+GGG 0.687899 5.3:+24560898 MS.gene58600:CDS
ATGTGATCATGGGAAGAATG+AGG 0.690153 5.3:-24560398 None:intergenic
ATAGTACTAATCAAGACCAG+AGG 0.717522 5.3:+24560792 MS.gene58600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAGAAAAAGCATTTTT+TGG + chr5.3:24561183-24561202 MS.gene58600:CDS 15.0%
!!! AAATCTTCTTATTTCACTAT+TGG - chr5.3:24560600-24560619 None:intergenic 20.0%
!!! TGATAGATCAATTTTAGTTT+GGG + chr5.3:24561288-24561307 MS.gene58600:CDS 20.0%
! CTGATAGAAAGATCAAAATT+TGG + chr5.3:24561116-24561135 MS.gene58600:CDS 25.0%
! CTGATTATAGTGTTAGATTA+TGG + chr5.3:24561458-24561477 MS.gene58600:CDS 25.0%
! TACTAAGGCTATATTATGTT+TGG + chr5.3:24561402-24561421 MS.gene58600:CDS 25.0%
! TATATTATGTTTGGTTGTGA+TGG + chr5.3:24561411-24561430 MS.gene58600:CDS 25.0%
! TGAAAAAAGTTATGCATGTT+TGG + chr5.3:24561495-24561514 MS.gene58600:CDS 25.0%
! TTCAAACAATATCAACAACT+TGG + chr5.3:24560646-24560665 MS.gene58600:CDS 25.0%
! TTGATTGTGATATCAAAGTT+TGG + chr5.3:24561641-24561660 MS.gene58600:CDS 25.0%
!! TTTTTCATCGAAAAATTACG+TGG + chr5.3:24560868-24560887 MS.gene58600:CDS 25.0%
!!! AAGAAAAAGCATTTTTTGGT+TGG + chr5.3:24561187-24561206 MS.gene58600:CDS 25.0%
!!! CTGTTTTTTGTTTTTGTTGT+TGG - chr5.3:24560376-24560395 None:intergenic 25.0%
!!! GTGATAGATCAATTTTAGTT+TGG + chr5.3:24561287-24561306 MS.gene58600:CDS 25.0%
AGCATTATTCGAAATTGTTC+CGG + chr5.3:24561322-24561341 MS.gene58600:CDS 30.0%
AGTGTTAGATTATGGAAAAG+AGG + chr5.3:24561466-24561485 MS.gene58600:CDS 30.0%
ATTCTGAATGAATGTGATCA+TGG - chr5.3:24560412-24560431 None:intergenic 30.0%
GAAAAACGATCATTGAATGT+TGG - chr5.3:24560842-24560861 None:intergenic 30.0%
GATTCCAAAAGTGGTAAAAA+TGG + chr5.3:24561568-24561587 MS.gene58600:CDS 30.0%
GGGATAGAACATTCAAAATA+TGG + chr5.3:24560990-24561009 MS.gene58600:CDS 30.0%
TAGAAAAGCTGTTAAGTGTT+TGG + chr5.3:24561534-24561553 MS.gene58600:CDS 30.0%
TTCTGAATGAATGTGATCAT+GGG - chr5.3:24560411-24560430 None:intergenic 30.0%
! AGTTCTTATCTTGTTTACAG+TGG + chr5.3:24561613-24561632 MS.gene58600:CDS 30.0%
! GAGACATAAGAAGTATACAT+GGG + chr5.3:24560898-24560917 MS.gene58600:CDS 30.0%
!! AACAACAATCATTGACTTGA+TGG - chr5.3:24560699-24560718 None:intergenic 30.0%
!! AATTGTTGTTTCATCTTCTG+GGG + chr5.3:24561075-24561094 MS.gene58600:CDS 30.0%
!! AGATGGATTGTTTCTTTACT+CGG + chr5.3:24560961-24560980 MS.gene58600:CDS 30.0%
!! CAATTGTTGTTTCATCTTCT+GGG + chr5.3:24561074-24561093 MS.gene58600:CDS 30.0%
!! GATGGATCAGTTTTATATTC+AGG + chr5.3:24561259-24561278 MS.gene58600:CDS 30.0%
AGAACCACTAAAGAGAAACT+TGG - chr5.3:24560576-24560595 None:intergenic 35.0%
ATAGCAGATGATTCCAAAAG+TGG + chr5.3:24561559-24561578 MS.gene58600:CDS 35.0%
ATAGTACTAATCAAGACCAG+AGG + chr5.3:24560792-24560811 MS.gene58600:CDS 35.0%
CAAAAGTGGTAAAAATGGTG+GGG + chr5.3:24561573-24561592 MS.gene58600:CDS 35.0%
CCAAAAGTGGTAAAAATGGT+GGG + chr5.3:24561572-24561591 MS.gene58600:CDS 35.0%
CTATGCATTTAAATAGCCTC+TGG - chr5.3:24560811-24560830 None:intergenic 35.0%
CTCATCAAGATCACAAAATC+CGG + chr5.3:24560738-24560757 MS.gene58600:CDS 35.0%
GCTTTAGCACTAAACAAAGA+TGG + chr5.3:24561241-24561260 MS.gene58600:CDS 35.0%
TCATCAAGATCACAAAATCC+GGG + chr5.3:24560739-24560758 MS.gene58600:CDS 35.0%
TCCAAAAGTGGTAAAAATGG+TGG + chr5.3:24561571-24561590 MS.gene58600:CDS 35.0%
TTGAAGCTTGAAGAGCAAAA+GGG - chr5.3:24560631-24560650 None:intergenic 35.0%
! AACTTGGTAGCAATCAGTAA+TGG + chr5.3:24560662-24560681 MS.gene58600:CDS 35.0%
! GGAGACATAAGAAGTATACA+TGG + chr5.3:24560897-24560916 MS.gene58600:CDS 35.0%
! TTGTTTCGATTTTCCAAACC+CGG - chr5.3:24560760-24560779 None:intergenic 35.0%
! TTTGAAGCTTGAAGAGCAAA+AGG - chr5.3:24560632-24560651 None:intergenic 35.0%
! TTTTGTGTGTAGCAACAACA+AGG - chr5.3:24560353-24560372 None:intergenic 35.0%
!! GCAATTGTTGTTTCATCTTC+TGG + chr5.3:24561073-24561092 MS.gene58600:CDS 35.0%
!! TTTGGTTGTGATGGATGATT+TGG + chr5.3:24561420-24561439 MS.gene58600:CDS 35.0%
!!! GCATTTTTTGGTTGGAACAT+TGG + chr5.3:24561195-24561214 MS.gene58600:CDS 35.0%
AAACTTGGAGTGTAGAGCAA+GGG - chr5.3:24560561-24560580 None:intergenic 40.0%
AAGATCACAAAATCCGGGTT+TGG + chr5.3:24560744-24560763 MS.gene58600:CDS 40.0%
ATGTGATCATGGGAAGAATG+AGG - chr5.3:24560401-24560420 None:intergenic 40.0%
CACTCCAAGTTTCTCTTTAG+TGG + chr5.3:24560569-24560588 MS.gene58600:CDS 40.0%
CGAAAAATTACGTGGAAGTC+AGG + chr5.3:24560876-24560895 MS.gene58600:CDS 40.0%
GACGAAAATATGGTCCTAGT+TGG + chr5.3:24561364-24561383 MS.gene58600:CDS 40.0%
GATTGTTGTGAAGTGAGAGA+AGG - chr5.3:24560482-24560501 None:intergenic 40.0%
! TAGGACCATATTTTCGTCGT+CGG - chr5.3:24561362-24561381 None:intergenic 40.0%
!! ATGGATGATTTGGTGTGTAG+TGG + chr5.3:24561430-24561449 MS.gene58600:CDS 40.0%
!! CCCACCATTTTTACCACTTT+TGG - chr5.3:24561575-24561594 None:intergenic 40.0%
!! TCTCTTGCTGTTTCGAAAGA+TGG + chr5.3:24560944-24560963 MS.gene58600:CDS 40.0%
!! TCTTCTGGGGTTGTTTATAC+AGG + chr5.3:24561088-24561107 MS.gene58600:CDS 40.0%
AGCCTTAGTATGCCCTCTTA+GGG - chr5.3:24561392-24561411 None:intergenic 45.0%
GAAACTTGGAGTGTAGAGCA+AGG - chr5.3:24560562-24560581 None:intergenic 45.0%
GAAGGAACAGAAGGTAGACT+TGG - chr5.3:24560464-24560483 None:intergenic 45.0%
GAAGTGAGAGAAGGAACAGA+AGG - chr5.3:24560473-24560492 None:intergenic 45.0%
GTTTCTTTACTCGGCTTCGT+GGG + chr5.3:24560970-24560989 MS.gene58600:CDS 45.0%
TGTAGAGCAAGGGATGAGAT+GGG - chr5.3:24560551-24560570 None:intergenic 45.0%
TGTTTCTTTACTCGGCTTCG+TGG + chr5.3:24560969-24560988 MS.gene58600:CDS 45.0%
! AGGACCATATTTTCGTCGTC+GGG - chr5.3:24561361-24561380 None:intergenic 45.0%
! TAGCCTTAGTATGCCCTCTT+AGG - chr5.3:24561393-24561412 None:intergenic 45.0%
!! GCATGTTTGGCTGTTTTGCA+AGG + chr5.3:24561508-24561527 MS.gene58600:CDS 45.0%
AGACCCCGACGACGAAAATA+TGG + chr5.3:24561354-24561373 MS.gene58600:CDS 50.0%
GTGTAGAGCAAGGGATGAGA+TGG - chr5.3:24560552-24560571 None:intergenic 50.0%
! GGACCATATTTTCGTCGTCG+GGG - chr5.3:24561360-24561379 None:intergenic 50.0%
! TATTTTCGTCGTCGGGGTCT+TGG - chr5.3:24561354-24561373 None:intergenic 50.0%
! TCCTAGTTGGTGCCCTAAGA+GGG + chr5.3:24561377-24561396 MS.gene58600:CDS 50.0%
! TGCCCTAAGAGGGCATACTA+AGG + chr5.3:24561387-24561406 MS.gene58600:CDS 50.0%
!! GGTGGGGTTGATGATGATGA+TGG + chr5.3:24561589-24561608 MS.gene58600:CDS 50.0%
! GTCCTAGTTGGTGCCCTAAG+AGG + chr5.3:24561376-24561395 MS.gene58600:CDS 55.0%
GTCGGGGTCTTGGTCTAGTC+CGG - chr5.3:24561344-24561363 None:intergenic 60.0%
! GCCCTCTTAGGGCACCAACT+AGG - chr5.3:24561381-24561400 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 24560332 24561687 24560332 ID=MS.gene58600
chr5.3 mRNA 24560332 24561687 24560332 ID=MS.gene58600.t1;Parent=MS.gene58600
chr5.3 exon 24560332 24561687 24560332 ID=MS.gene58600.t1.exon1;Parent=MS.gene58600.t1
chr5.3 CDS 24560332 24561687 24560332 ID=cds.MS.gene58600.t1;Parent=MS.gene58600.t1
Gene Sequence

>MS.gene58600

ATGGTACTTCTTCAATATCCTTGTTGTTGCTACACACAAAACCAACAACAAAAACAAAAAACAGAACCTCATTCTTCCCATGATCACATTCATTCAGAATCATCAACTTCTTCATCTCTTTCTTCACAACCAAGTCTACCTTCTGTTCCTTCTCTCACTTCACAACAATCTCAAACTACACATCACAAACTTCTAACAACAATCAATCACTCTTCACCCATCTCATCCCTTGCTCTACACTCCAAGTTTCTCTTTAGTGGTTCTTCCAATAGTGAAATAAGAAGATTTGATAAAGACCCTTTTGCTCTTCAAGCTTCAAACAATATCAACAACTTGGTAGCAATCAGTAATGGATCAAAATCAACCATCAAGTCAATGATTGTTGTTAATGATATGTTATTCAGTGCTCATCAAGATCACAAAATCCGGGTTTGGAAAATCGAAACAACGACAGCTAGTAATAGTACTAATCAAGACCAGAGGCTATTTAAATGCATAGCAACACTTCCAACATTCAATGATCGTTTTTCGAAGCTTTTTTCATCGAAAAATTACGTGGAAGTCAGGAGACATAAGAAGTATACATGGGTGAATCATATTGATGCTGTTTCTTCTCTTGCTGTTTCGAAAGATGGATTGTTTCTTTACTCGGCTTCGTGGGATAGAACATTCAAAATATGGAGAGTTTCAGATTTCAAGTGTTTAGAGTCAGTGAAAAATGCACATGAAGATGCAATCAATGCAATTGTTGTTTCATCTTCTGGGGTTGTTTATACAGGTTCAGCTGATAGAAAGATCAAAATTTGGAAGAAAAAAAATCTTGAAGAAGATGAGAAAATGAAAAGTAAAAGTAAAAAGAAAAAGCATTTTTTGGTTGGAACATTGGAGAAGCATAAATCAGCTGTGAATGCTTTAGCACTAAACAAAGATGGATCAGTTTTATATTCAGGTGCTTGTGATAGATCAATTTTAGTTTGGGAAAAAAGTAATAGCATTATTCGAAATTGTTCCGGACTAGACCAAGACCCCGACGACGAAAATATGGTCCTAGTTGGTGCCCTAAGAGGGCATACTAAGGCTATATTATGTTTGGTTGTGATGGATGATTTGGTGTGTAGTGGATCAGCTGATTATAGTGTTAGATTATGGAAAAGAGGAATTGATGAAAAAAGTTATGCATGTTTGGCTGTTTTGCAAGGTCATAGAAAAGCTGTTAAGTGTTTGGCTATAGCAGATGATTCCAAAAGTGGTAAAAATGGTGGGGTTGATGATGATGATGGTAGTTCTTATCTTGTTTACAGTGGTAGTCTTGATTGTGATATCAAAGTTTGGCAAATACGCGTTCCCTTAATTTGA
Protein sequence

>MS.gene58600.t1

MVLLQYPCCCYTQNQQQKQKTEPHSSHDHIHSESSTSSSLSSQPSLPSVPSLTSQQSQTTHHKLLTTINHSSPISSLALHSKFLFSGSSNSEIRRFDKDPFALQASNNINNLVAISNGSKSTIKSMIVVNDMLFSAHQDHKIRVWKIETTTASNSTNQDQRLFKCIATLPTFNDRFSKLFSSKNYVEVRRHKKYTWVNHIDAVSSLAVSKDGLFLYSASWDRTFKIWRVSDFKCLESVKNAHEDAINAIVVSSSGVVYTGSADRKIKIWKKKNLEEDEKMKSKSKKKKHFLVGTLEKHKSAVNALALNKDGSVLYSGACDRSILVWEKSNSIIRNCSGLDQDPDDENMVLVGALRGHTKAILCLVVMDDLVCSGSADYSVRLWKRGIDEKSYACLAVLQGHRKAVKCLAIADDSKSGKNGGVDDDDGSSYLVYSGSLDCDIKVWQIRVPLI