Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58849.t1 | RHN47713.1 | 98.4 | 251 | 3 | 1 | 1 | 251 | 1 | 250 | 6.80E-111 | 410.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58849.t1 | O80674 | 68.5 | 178 | 52 | 3 | 57 | 232 | 49 | 224 | 4.4e-58 | 226.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58849.t1 | A0A396H4N2 | 98.4 | 251 | 3 | 1 | 1 | 251 | 1 | 250 | 4.9e-111 | 410.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene58849.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene58849 | 0.821939 | 3.09E-53 | -1.69E-46 |
| MS.gene050707 | MS.gene58849 | 0.857638 | 1.42E-62 | -1.69E-46 |
| MS.gene050710 | MS.gene58849 | 0.809985 | 1.44E-50 | -1.69E-46 |
| MS.gene054593 | MS.gene58849 | 0.803687 | 3.10E-49 | -1.69E-46 |
| MS.gene054850 | MS.gene58849 | 0.908996 | 9.28E-82 | -1.69E-46 |
| MS.gene054941 | MS.gene58849 | 0.805875 | 1.08E-49 | -1.69E-46 |
| MS.gene05652 | MS.gene58849 | 0.842385 | 2.67E-58 | -1.69E-46 |
| MS.gene057356 | MS.gene58849 | 0.804706 | 1.90E-49 | -1.69E-46 |
| MS.gene057669 | MS.gene58849 | 0.820133 | 8.06E-53 | -1.69E-46 |
| MS.gene058128 | MS.gene58849 | 0.847034 | 1.49E-59 | -1.69E-46 |
| MS.gene058789 | MS.gene58849 | 0.812713 | 3.69E-51 | -1.69E-46 |
| MS.gene058853 | MS.gene58849 | 0.85106 | 1.13E-60 | -1.69E-46 |
| MS.gene05926 | MS.gene58849 | 0.8059 | 1.07E-49 | -1.69E-46 |
| MS.gene059616 | MS.gene58849 | 0.824527 | 7.69E-54 | -1.69E-46 |
| MS.gene059905 | MS.gene58849 | 0.843635 | 1.24E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58849.t1 | MTR_7g089400 | 87.200 | 125 | 15 | 1 | 1 | 125 | 1 | 124 | 1.51e-74 | 223 |
| MS.gene58849.t1 | MTR_1g093750 | 49.215 | 191 | 90 | 3 | 59 | 243 | 141 | 330 | 1.22e-47 | 161 |
| MS.gene58849.t1 | MTR_3g070970 | 35.317 | 252 | 137 | 6 | 14 | 243 | 7 | 254 | 4.06e-42 | 144 |
| MS.gene58849.t1 | MTR_2g091190 | 46.369 | 179 | 89 | 3 | 71 | 243 | 54 | 231 | 4.00e-34 | 123 |
| MS.gene58849.t1 | MTR_2g015890 | 38.636 | 176 | 99 | 3 | 71 | 241 | 62 | 233 | 5.76e-31 | 115 |
| MS.gene58849.t1 | MTR_4g079760 | 42.021 | 188 | 92 | 5 | 68 | 243 | 60 | 242 | 3.08e-28 | 108 |
| MS.gene58849.t1 | MTR_2g015890 | 41.880 | 117 | 64 | 1 | 71 | 187 | 62 | 174 | 9.66e-25 | 98.2 |
| MS.gene58849.t1 | MTR_5g066080 | 39.080 | 174 | 78 | 3 | 59 | 206 | 19 | 190 | 1.22e-23 | 96.3 |
| MS.gene58849.t1 | MTR_5g032375 | 32.093 | 215 | 118 | 6 | 32 | 233 | 7 | 206 | 5.24e-22 | 90.9 |
| MS.gene58849.t1 | MTR_4g087920 | 37.805 | 164 | 97 | 4 | 70 | 230 | 69 | 230 | 9.58e-22 | 91.7 |
| MS.gene58849.t1 | MTR_2g015890 | 34.058 | 138 | 82 | 3 | 109 | 241 | 2 | 135 | 1.77e-15 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58849.t1 | AT2G41130 | 62.100 | 219 | 77 | 5 | 18 | 233 | 10 | 225 | 4.93e-81 | 244 |
| MS.gene58849.t1 | AT3G56770 | 61.140 | 193 | 61 | 4 | 70 | 250 | 39 | 229 | 5.85e-73 | 222 |
| MS.gene58849.t1 | AT1G68810 | 45.729 | 199 | 96 | 3 | 57 | 243 | 151 | 349 | 2.10e-46 | 159 |
| MS.gene58849.t1 | AT3G25710 | 57.857 | 140 | 57 | 1 | 72 | 211 | 128 | 265 | 6.49e-44 | 151 |
| MS.gene58849.t1 | AT2G40200 | 41.579 | 190 | 94 | 4 | 71 | 245 | 58 | 245 | 7.88e-35 | 125 |
| MS.gene58849.t1 | AT4G38070 | 34.807 | 181 | 102 | 5 | 26 | 206 | 50 | 214 | 2.18e-22 | 92.8 |
Find 0 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAGATTCAGAATAATATT+GGG | + | 50879:87257-87276 | MS.gene58849:CDS | 20.0% |
| !! | AATGATGAAAATAATAACCT+TGG | - | 50879:87566-87585 | None:intergenic | 20.0% |
| !! | AATTATTTGCAAATTAAGCT+AGG | + | 50879:88132-88151 | MS.gene58849:intron | 20.0% |
| !! | ATTATTTGCAAATTAAGCTA+GGG | + | 50879:88133-88152 | MS.gene58849:intron | 20.0% |
| !! | GATGTTCCAAAAAATAATTA+AGG | - | 50879:87913-87932 | None:intergenic | 20.0% |
| !! | TCATTGTTAATTATTTGTCT+TGG | + | 50879:87581-87600 | MS.gene58849:intron | 20.0% |
| !!! | CTAGATCCTTAATTATTTTT+TGG | + | 50879:87904-87923 | MS.gene58849:intron | 20.0% |
| !!! | GCTTAATTTGCAAATAATTT+TGG | - | 50879:88131-88150 | None:intergenic | 20.0% |
| !!! | GTAATAGTTATTGTTGTTAT+CGG | - | 50879:87392-87411 | None:intergenic | 20.0% |
| !!! | TTGGATTACATATTTTTTGT+AGG | - | 50879:88062-88081 | None:intergenic | 20.0% |
| ! | AGTAATTTCAGAAACTTCAA+AGG | - | 50879:87419-87438 | None:intergenic | 25.0% |
| ! | CAACAGATTCAGAATAATAT+TGG | + | 50879:87256-87275 | MS.gene58849:CDS | 25.0% |
| ! | CTTAATTACTAAAGTGAGAA+AGG | - | 50879:87802-87821 | None:intergenic | 25.0% |
| ! | GTAATTTCAGAAACTTCAAA+GGG | - | 50879:87418-87437 | None:intergenic | 25.0% |
| ! | TAATTTCAGAAACTTCAAAG+GGG | - | 50879:87417-87436 | None:intergenic | 25.0% |
| ! | TAGTCATGTATAAAACTTGA+TGG | - | 50879:88222-88241 | None:intergenic | 25.0% |
| ! | TTGAGTATTCAAAGAATGAA+GGG | - | 50879:87327-87346 | None:intergenic | 25.0% |
| !! | ACATGTTTTCACTCTTTTTT+TGG | - | 50879:87747-87766 | None:intergenic | 25.0% |
| !! | TCAATTTTACAAAACCTACA+AGG | + | 50879:88340-88359 | MS.gene58849:intron | 25.0% |
| !! | TTTTGTTCTTTTGTTCTCAA+AGG | + | 50879:87647-87666 | MS.gene58849:intron | 25.0% |
| !!! | TTGTAGGTTTTGTAAAATTG+AGG | - | 50879:88341-88360 | None:intergenic | 25.0% |
| AAACACACAATTATCTTTGC+TGG | - | 50879:87688-87707 | None:intergenic | 30.0% | |
| AAACCATTGTGATTTAGAAG+AGG | - | 50879:88368-88387 | None:intergenic | 30.0% | |
| GATTCAGAATAATATTGGGA+TGG | + | 50879:87261-87280 | MS.gene58849:CDS | 30.0% | |
| GTTGAGTATTCAAAGAATGA+AGG | - | 50879:87328-87347 | None:intergenic | 30.0% | |
| TGATGAGTGATTAAGTTCAT+TGG | - | 50879:88081-88100 | None:intergenic | 30.0% | |
| ! | ATTTTCTTCAGAATTCACTC+AGG | + | 50879:88892-88911 | MS.gene58849:CDS | 30.0% |
| ! | CTTTAGTAATTAAGTTGCCA+TGG | + | 50879:87808-87827 | MS.gene58849:intron | 30.0% |
| !! | AGATATGACTGTTATTTCGT+CGG | - | 50879:88661-88680 | None:intergenic | 30.0% |
| !! | TCATTTTTGTCTCAAAACCT+AGG | - | 50879:88445-88464 | None:intergenic | 30.0% |
| !!! | GTTTTCACTCTTTTTTTGGA+TGG | - | 50879:87743-87762 | None:intergenic | 30.0% |
| AAAAACTCTCAGAGCTGAAA+TGG | + | 50879:88794-88813 | MS.gene58849:CDS | 35.0% | |
| ATAATAACCTTGGAGTTGCA+AGG | - | 50879:87556-87575 | None:intergenic | 35.0% | |
| CTAGTACATGTTTGCATATG+TGG | - | 50879:88403-88422 | None:intergenic | 35.0% | |
| GAAATAACAGTCATATCTGC+CGG | + | 50879:88663-88682 | MS.gene58849:CDS | 35.0% | |
| TAATAACCTTGGAGTTGCAA+GGG | - | 50879:87555-87574 | None:intergenic | 35.0% | |
| TCAATCTTGTCACAAAACCT+AGG | - | 50879:88482-88501 | None:intergenic | 35.0% | |
| TCTTATGAGTTTAGCACAAG+TGG | + | 50879:87977-87996 | MS.gene58849:intron | 35.0% | |
| TGATGAAGAAAGCAAAACCT+AGG | + | 50879:88462-88481 | MS.gene58849:intron | 35.0% | |
| ! | AGGCATATTTTCACTCATTC+TGG | - | 50879:87782-87801 | None:intergenic | 35.0% |
| ! | AGTATCGAACTCAAAACATC+CGG | - | 50879:88037-88056 | None:intergenic | 35.0% |
| ! | GTAAAGTACTACATGTTCCA+TGG | - | 50879:87828-87847 | None:intergenic | 35.0% |
| ! | TGATTGATTTCAGACAGACA+AGG | + | 50879:88545-88564 | MS.gene58849:intron | 35.0% |
| !!! | TTTCAGCTCTGAGAGTTTTT+AGG | - | 50879:88794-88813 | None:intergenic | 35.0% |
| !!! | TTTTTAGGTGAAGAGACTTG+AGG | - | 50879:88779-88798 | None:intergenic | 35.0% |
| ATGAAGGGTTGTTAGCAACA+AGG | - | 50879:87312-87331 | None:intergenic | 40.0% | |
| ATGAGTTTAGCACAAGTGGT+CGG | + | 50879:87981-88000 | MS.gene58849:intron | 40.0% | |
| ATTTAGAAGAGGCTCCTTGT+AGG | - | 50879:88357-88376 | None:intergenic | 40.0% | |
| CTTGAGGATTTCGATTAGGT+CGG | - | 50879:88763-88782 | None:intergenic | 40.0% | |
| GAATTCACTCAGGTCTTTGT+TGG | + | 50879:88902-88921 | MS.gene58849:CDS | 40.0% | |
| GAGACTTGAGGATTTCGATT+AGG | - | 50879:88767-88786 | None:intergenic | 40.0% | |
| GAGCCTCTTCTAAATCACAA+TGG | + | 50879:88362-88381 | MS.gene58849:intron | 40.0% | |
| GTTGTAACGATAGGTCGAAA+AGG | + | 50879:88937-88956 | MS.gene58849:CDS | 40.0% | |
| TCTTTGTTGGATAGGTCTAG+TGG | + | 50879:88915-88934 | MS.gene58849:CDS | 40.0% | |
| TGATGAAGAAAGCAAAGCCT+AGG | + | 50879:88425-88444 | MS.gene58849:intron | 40.0% | |
| TTAAGCTAGGGAAGTCACTT+AGG | + | 50879:88145-88164 | MS.gene58849:intron | 40.0% | |
| TTGAGGATTTCGATTAGGTC+GGG | - | 50879:88762-88781 | None:intergenic | 40.0% | |
| TTTCAGAAACTTCAAAGGGG+TGG | - | 50879:87414-87433 | None:intergenic | 40.0% | |
| TTTCGATTAGGTCGGGAATT+AGG | - | 50879:88755-88774 | None:intergenic | 40.0% | |
| ! | AGGGAAGTCACTTAGGTTTT+AGG | + | 50879:88152-88171 | MS.gene58849:intron | 40.0% |
| ! | TAGCTTTTGCAAAATCGTGG+TGG | - | 50879:88313-88332 | None:intergenic | 40.0% |
| ! | TCGTAGCTTTTGCAAAATCG+TGG | - | 50879:88316-88335 | None:intergenic | 40.0% |
| ! | TGTCTGAAGTGGTGTATATC+CGG | + | 50879:88015-88034 | MS.gene58849:intron | 40.0% |
| ! | TGTTGCATCATAGTGCTACT+AGG | - | 50879:87352-87371 | None:intergenic | 40.0% |
| !! | AGCTGCTTTGAAGAATCACA+AGG | + | 50879:87468-87487 | MS.gene58849:CDS | 40.0% |
| !! | GACTGTTATTTCGTCGGTTT+CGG | - | 50879:88655-88674 | None:intergenic | 40.0% |
| AGAGCTCTATCTTGCTGAGA+GGG | - | 50879:87448-87467 | None:intergenic | 45.0% | |
| AGGAAGCTGAGAAGAGAAGA+AGG | + | 50879:87488-87507 | MS.gene58849:CDS | 45.0% | |
| CACTCAGGTCTTTGTTGGAT+AGG | + | 50879:88907-88926 | MS.gene58849:CDS | 45.0% | |
| GAATATGAGTCTGCCTTCGT+CGG | - | 50879:88700-88719 | None:intergenic | 45.0% | |
| GATTAGGTCGGGAATTAGGT+CGG | - | 50879:88751-88770 | None:intergenic | 45.0% | |
| GGAAGCTGAGAAGAGAAGAA+GGG | + | 50879:87489-87508 | MS.gene58849:CDS | 45.0% | |
| GGTCTAGTGGTTGTAACGAT+AGG | + | 50879:88928-88947 | MS.gene58849:CDS | 45.0% | |
| TAGAGCTCTATCTTGCTGAG+AGG | - | 50879:87449-87468 | None:intergenic | 45.0% | |
| TGCGTAATGTGTGTCTGAAG+TGG | + | 50879:88004-88023 | MS.gene58849:intron | 45.0% | |
| !! | GTTATTTCGTCGGTTTCGGA+TGG | - | 50879:88651-88670 | None:intergenic | 45.0% |
| !!! | TTTTATTTTTTTACTATTTA+AGG | - | 50879:88108-88127 | None:intergenic | 5.0% |
| ACGTGTTCTTCCACCAAGTG+TGG | - | 50879:88820-88839 | None:intergenic | 50.0% | |
| AGAGCTGAAATGGCCACACT+TGG | + | 50879:88804-88823 | MS.gene58849:CDS | 50.0% | |
| CTTGGAGTTGCAAGGGAGAA+GGG | - | 50879:87548-87567 | None:intergenic | 50.0% | |
| TTCGTCGGAGATGTCATTGC+CGG | - | 50879:88685-88704 | None:intergenic | 50.0% | |
| ! | AAGGGAGAAGGGTACGAAGA+TGG | - | 50879:87537-87556 | None:intergenic | 50.0% |
| ! | GAAGGGTACGAAGATGGTCA+AGG | - | 50879:87531-87550 | None:intergenic | 50.0% |
| !! | ATAGGTCGAAAAGGCGTCGA+GGG | + | 50879:88946-88965 | MS.gene58849:CDS | 50.0% |
| CCTTCTCCCTTGCAACTCCA+AGG | + | 50879:87546-87565 | MS.gene58849:CDS | 55.0% | |
| CCTTGGAGTTGCAAGGGAGA+AGG | - | 50879:87549-87568 | None:intergenic | 55.0% | |
| GCTGAAATGGCCACACTTGG+TGG | + | 50879:88807-88826 | MS.gene58849:CDS | 55.0% | |
| GGCAATGACATCTCCGACGA+AGG | + | 50879:88684-88703 | MS.gene58849:CDS | 55.0% | |
| !! | GATAGGTCGAAAAGGCGTCG+AGG | + | 50879:88945-88964 | MS.gene58849:CDS | 55.0% |
| !! | GTCGAAAAGGCGTCGAGGGT+TGG | + | 50879:88950-88969 | MS.gene58849:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50879 | gene | 87247 | 88992 | 87247 | ID=MS.gene58849 |
| 50879 | mRNA | 87247 | 88992 | 87247 | ID=MS.gene58849.t1;Parent=MS.gene58849 |
| 50879 | exon | 87247 | 87567 | 87247 | ID=MS.gene58849.t1.exon1;Parent=MS.gene58849.t1 |
| 50879 | CDS | 87247 | 87567 | 87247 | ID=cds.MS.gene58849.t1;Parent=MS.gene58849.t1 |
| 50879 | exon | 88558 | 88992 | 88558 | ID=MS.gene58849.t1.exon2;Parent=MS.gene58849.t1 |
| 50879 | CDS | 88558 | 88992 | 88558 | ID=cds.MS.gene58849.t1;Parent=MS.gene58849.t1 |
| Gene Sequence |
| Protein sequence |
