AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58950


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58950.t1 MTR_2g020360 76.259 139 32 1 1 138 1 139 1.55e-68 203
MS.gene58950.t1 MTR_2g020360 76.259 139 32 1 1 138 1 139 6.51e-68 202
MS.gene58950.t1 MTR_2g020360 78.862 123 26 0 16 138 17 139 7.01e-65 194
MS.gene58950.t1 MTR_4g118750 72.308 130 16 3 12 139 13 124 1.45e-54 168
MS.gene58950.t1 MTR_8g099920 48.936 141 63 4 1 137 1 136 1.17e-35 120
MS.gene58950.t1 MTR_8g099920 48.252 143 63 5 1 137 1 138 1.03e-33 115
MS.gene58950.t1 MTR_1g081920 65.385 78 22 2 57 132 12 86 2.43e-24 90.1
MS.gene58950.t1 MTR_8g099920 44.762 105 47 5 1 99 1 100 7.16e-14 63.9
MS.gene58950.t1 MTR_8g073750 93.548 31 2 0 86 116 23 53 4.73e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58950.t1 AT4G25050 53.957 139 58 1 1 139 1 133 2.32e-40 132
MS.gene58950.t1 AT4G25050 49.669 151 58 2 1 139 1 145 7.98e-37 123
MS.gene58950.t1 AT1G54630 53.237 139 60 3 1 138 1 135 1.18e-36 122
MS.gene58950.t1 AT1G54580 54.783 115 44 1 24 138 29 135 2.53e-35 119
MS.gene58950.t1 AT5G27200 50.000 138 54 4 3 139 16 139 1.06e-34 117
MS.gene58950.t1 AT3G05020 48.889 135 66 2 6 137 2 136 9.72e-34 115
MS.gene58950.t1 AT1G54630 48.485 99 46 3 1 98 1 95 5.08e-17 71.6

Find 36 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAAAGGGAAACTGATTT+TGG 0.126186 8.3:-7909119 None:intergenic
ATCAGTTTCCCTTTCAATTA+AGG 0.135610 8.3:+7909124 MS.gene58950:CDS
AGCTCCAAGTGCAGAAAATT+TGG 0.215999 8.3:-7909966 None:intergenic
CAATCATGTCTGCTGCTTCT+TGG 0.254760 8.3:-7910734 None:intergenic
ATGGGACTTGAGGAGGAATT+TGG 0.301410 8.3:+7910669 MS.gene58950:CDS
TCAGTTTCCCTTTCAATTAA+GGG 0.363477 8.3:+7909125 MS.gene58950:CDS
CATCCATTACATTGCAGTCT+AGG 0.375528 8.3:+7909159 MS.gene58950:CDS
CTCAGCATTCAAACAATCTC+TGG 0.432166 8.3:+7908992 MS.gene58950:CDS
GGCATTCCCCTTAATTGAAA+GGG 0.445874 8.3:-7909132 None:intergenic
CTTGGACGGTGGTAATGGAC+TGG 0.447324 8.3:-7910716 None:intergenic
TGCTTCTTGGACGGTGGTAA+TGG 0.455810 8.3:-7910721 None:intergenic
CCAGACGAGTCCTCTGTCAC+TGG 0.476490 8.3:+7909938 MS.gene58950:CDS
ATCCATTACATTGCAGTCTA+GGG 0.489304 8.3:+7909160 MS.gene58950:CDS
TACCCTAGACTGCAATGTAA+TGG 0.496902 8.3:-7909162 None:intergenic
CTGTAGGTTGAGATTGTGAT+GGG 0.514505 8.3:+7910651 MS.gene58950:intron
AGGCATTCCCCTTAATTGAA+AGG 0.514759 8.3:-7909133 None:intergenic
CATGTCTGCTGCTTCTTGGA+CGG 0.518674 8.3:-7910730 None:intergenic
CAGTTTCCCTTTCAATTAAG+GGG 0.519673 8.3:+7909126 MS.gene58950:CDS
TACACAATGGCATCCATCAC+AGG 0.524640 8.3:+7908954 None:intergenic
GAATGCTGAGACGGAGAATG+AGG 0.525070 8.3:-7908979 None:intergenic
CTGCAATGTAATGGATGGAA+AGG 0.533896 8.3:-7909153 None:intergenic
GAAAGCAGAAACTGTAAAGA+AGG 0.538034 8.3:+7909883 MS.gene58950:CDS
CTAGACTGCAATGTAATGGA+TGG 0.538900 8.3:-7909158 None:intergenic
CCAGTGACAGAGGACTCGTC+TGG 0.547733 8.3:-7909938 None:intergenic
AGATTGTTTGAATGCTGAGA+CGG 0.570818 8.3:-7908988 None:intergenic
CCTGTAGGTTGAGATTGTGA+TGG 0.575547 8.3:+7910650 MS.gene58950:intron
TGGAGCTGACTCTCTCGATA+CGG 0.589016 8.3:+7909982 MS.gene58950:CDS
GGAGAATGAGGTTCCTGTGA+TGG 0.592118 8.3:-7908967 None:intergenic
GTCTGCTGCTTCTTGGACGG+TGG 0.592160 8.3:-7910727 None:intergenic
TGAGATTGTGATGGGACTTG+AGG 0.596018 8.3:+7910659 MS.gene58950:CDS
GGGTTAGAGAATCTAGCACC+AGG 0.601875 8.3:-7909098 None:intergenic
TTGGACGGTGGTAATGGACT+GGG 0.607190 8.3:-7910715 None:intergenic
GATTGTGATGGGACTTGAGG+AGG 0.609784 8.3:+7910662 MS.gene58950:CDS
ATTCACATCTAACAGGTACC+TGG 0.634844 8.3:+7909080 MS.gene58950:intron
TTTGGACTCTCCAGTGACAG+AGG 0.640603 8.3:-7909948 None:intergenic
GCACATGTAACTTGAAAGCG+AGG 0.680439 8.3:-7909185 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAATAAAAGAAAATATA+GGG - chr8.3:7909247-7909266 None:intergenic 10.0%
!!! TAATAATTTTAGTTTATACA+TGG - chr8.3:7909399-7909418 None:intergenic 10.0%
!! AATATTACGTACATATTATA+AGG + chr8.3:7909423-7909442 MS.gene58950:intron 15.0%
!! AATGATATTCTAAATAGAAA+AGG - chr8.3:7910420-7910439 None:intergenic 15.0%
!! ATATTAATAGTAAAAATCCA+AGG - chr8.3:7909858-7909877 None:intergenic 15.0%
!! ATGATATTCTAAATAGAAAA+GGG - chr8.3:7910419-7910438 None:intergenic 15.0%
!! CCAAAAATAAAAGAAAATAT+AGG - chr8.3:7909248-7909267 None:intergenic 15.0%
!!! ATTATGTTTTTTAATCTACA+TGG + chr8.3:7910445-7910464 MS.gene58950:intron 15.0%
!!! CCTATATTTTCTTTTATTTT+TGG + chr8.3:7909245-7909264 MS.gene58950:intron 15.0%
!! ACAAACAATTCTATTCATTA+TGG + chr8.3:7909017-7909036 MS.gene58950:intron 20.0%
!! AGTTGTATATTTGAATTGTT+TGG + chr8.3:7909511-7909530 MS.gene58950:intron 20.0%
!! GTTGTATATTTGAATTGTTT+GGG + chr8.3:7909512-7909531 MS.gene58950:intron 20.0%
!! TAGAAAAAGATGTTCAAAAT+TGG - chr8.3:7910311-7910330 None:intergenic 20.0%
!! TCATGGATCAAATTAAAAAT+TGG - chr8.3:7909320-7909339 None:intergenic 20.0%
!! TTGAACAATATATTTGATGT+TGG + chr8.3:7909743-7909762 MS.gene58950:intron 20.0%
!!! AAATTTGTTCACATTGAAAT+TGG - chr8.3:7909450-7909469 None:intergenic 20.0%
!!! AATTTTGAACATCTTTTTCT+AGG + chr8.3:7910310-7910329 MS.gene58950:intron 20.0%
!!! ATTCATTATGGTTTAATTTG+TGG + chr8.3:7909029-7909048 MS.gene58950:intron 20.0%
!!! GGATTTTTACTATTAATATC+AGG + chr8.3:7909859-7909878 MS.gene58950:intron 20.0%
! AATAACAACCTAATGAGAAA+TGG - chr8.3:7910220-7910239 None:intergenic 25.0%
! ATTTGATGTTGGAATTATCA+AGG + chr8.3:7909754-7909773 MS.gene58950:intron 25.0%
! TGACATTGTCAAAAAACAAT+TGG + chr8.3:7909910-7909929 MS.gene58950:CDS 25.0%
! TGGAATAAAACAAAACATCA+TGG - chr8.3:7909337-7909356 None:intergenic 25.0%
! TTAAAATCAGAAGACAGTTA+TGG + chr8.3:7910264-7910283 MS.gene58950:intron 25.0%
! TTAGAAAAGATAAACAACAG+AGG - chr8.3:7910595-7910614 None:intergenic 25.0%
!!! ATTTTATTTTTGTCTCTCCA+GGG + chr8.3:7909373-7909392 MS.gene58950:intron 25.0%
ATCAGTTTCCCTTTCAATTA+AGG + chr8.3:7909124-7909143 MS.gene58950:CDS 30.0%
TATGCTAATTCACATCTAAC+AGG + chr8.3:7909073-7909092 MS.gene58950:intron 30.0%
TCAGTTTCCCTTTCAATTAA+GGG + chr8.3:7909125-7909144 MS.gene58950:CDS 30.0%
!! ATTGAAAGGGAAACTGATTT+TGG - chr8.3:7909122-7909141 None:intergenic 30.0%
!! TTGAAAGGGAAACTGATTTT+GGG - chr8.3:7909121-7909140 None:intergenic 30.0%
!!! CATTTTATTTTTGTCTCTCC+AGG + chr8.3:7909372-7909391 MS.gene58950:intron 30.0%
!!! TAATGCTATCTTGCTGTTTT+TGG + chr8.3:7910162-7910181 MS.gene58950:intron 30.0%
AAATGTTCTGCAGACATACA+TGG - chr8.3:7909357-7909376 None:intergenic 35.0%
AAATGTTGCTAGTTCAGAAG+AGG + chr8.3:7909614-7909633 MS.gene58950:intron 35.0%
AGACTTGTCAAATTTCCTGT+AGG + chr8.3:7910635-7910654 MS.gene58950:intron 35.0%
AGATTGTTTGAATGCTGAGA+CGG - chr8.3:7908991-7909010 None:intergenic 35.0%
AGGGAGTAAAAGAGTTAAGA+GGG - chr8.3:7909228-7909247 None:intergenic 35.0%
ATCCATTACATTGCAGTCTA+GGG + chr8.3:7909160-7909179 MS.gene58950:CDS 35.0%
CACACAGAATCAAATATCAG+AGG - chr8.3:7909790-7909809 None:intergenic 35.0%
CAGTTTCCCTTTCAATTAAG+GGG + chr8.3:7909126-7909145 MS.gene58950:CDS 35.0%
CTCTGATATTTGATTCTGTG+TGG + chr8.3:7909788-7909807 MS.gene58950:intron 35.0%
GAAAGCAGAAACTGTAAAGA+AGG + chr8.3:7909883-7909902 MS.gene58950:CDS 35.0%
TACTCGATCCATTTCTCATT+AGG + chr8.3:7910209-7910228 MS.gene58950:intron 35.0%
TAGGGAGTAAAAGAGTTAAG+AGG - chr8.3:7909229-7909248 None:intergenic 35.0%
TATTGCCTTATCTAAGTGTG+TGG + chr8.3:7910113-7910132 MS.gene58950:intron 35.0%
TCATGTACTTCTTGCTTTCT+GGG + chr8.3:7910136-7910155 MS.gene58950:intron 35.0%
TGTCCTTTAGTATATTGCTG+TGG + chr8.3:7910341-7910360 MS.gene58950:intron 35.0%
TTGCCACAGCAATATACTAA+AGG - chr8.3:7910347-7910366 None:intergenic 35.0%
!! AAGAGTTAAGAGGGTTTTAG+AGG - chr8.3:7909219-7909238 None:intergenic 35.0%
!!! TTTTAGTTTATACATGGCCC+TGG - chr8.3:7909393-7909412 None:intergenic 35.0%
AGCTCCAAGTGCAGAAAATT+TGG - chr8.3:7909969-7909988 None:intergenic 40.0%
AGGCATTCCCCTTAATTGAA+AGG - chr8.3:7909136-7909155 None:intergenic 40.0%
ATTCACATCTAACAGGTACC+TGG + chr8.3:7909080-7909099 MS.gene58950:intron 40.0%
CATCCATTACATTGCAGTCT+AGG + chr8.3:7909159-7909178 MS.gene58950:CDS 40.0%
CATGACCACACACTTAGATA+AGG - chr8.3:7910121-7910140 None:intergenic 40.0%
CTCAGCATTCAAACAATCTC+TGG + chr8.3:7908992-7909011 MS.gene58950:CDS 40.0%
CTGTTGTGATATAAACGCCT+TGG + chr8.3:7909838-7909857 MS.gene58950:intron 40.0%
GGCATTCCCCTTAATTGAAA+GGG - chr8.3:7909135-7909154 None:intergenic 40.0%
GTCATGTACTTCTTGCTTTC+TGG + chr8.3:7910135-7910154 MS.gene58950:intron 40.0%
TACCCTAGACTGCAATGTAA+TGG - chr8.3:7909165-7909184 None:intergenic 40.0%
TCATGCTGAAGTTTCCACTT+AGG - chr8.3:7910543-7910562 None:intergenic 40.0%
! CTAGACTGCAATGTAATGGA+TGG - chr8.3:7909161-7909180 None:intergenic 40.0%
! CTGTAGGTTGAGATTGTGAT+GGG + chr8.3:7910651-7910670 MS.gene58950:intron 40.0%
! GAGTCCAAATTTTCTGCACT+TGG + chr8.3:7909962-7909981 MS.gene58950:CDS 40.0%
!! AGAGGAGAATGGATTTGTTG+TGG + chr8.3:7909632-7909651 MS.gene58950:intron 40.0%
!! CTGCAATGTAATGGATGGAA+AGG - chr8.3:7909156-7909175 None:intergenic 40.0%
AACCACTTAAGCTGCCTAAG+TGG + chr8.3:7910526-7910545 MS.gene58950:intron 45.0%
ATGGGACTTGAGGAGGAATT+TGG + chr8.3:7910669-7910688 MS.gene58950:CDS 45.0%
CAGGGTGAGAAAAGCAAGAA+GGG - chr8.3:7910499-7910518 None:intergenic 45.0%
CCATCACAATCTCAACCTAC+AGG - chr8.3:7910653-7910672 None:intergenic 45.0%
CTTAAGTGGTTCAAGAGGCA+GGG - chr8.3:7910517-7910536 None:intergenic 45.0%
GCACATGTAACTTGAAAGCG+AGG - chr8.3:7909188-7909207 None:intergenic 45.0%
GCTAGTTCAGAAGAGGAGAA+TGG + chr8.3:7909621-7909640 MS.gene58950:intron 45.0%
TTCCACTTAGGCAGCTTAAG+TGG - chr8.3:7910531-7910550 None:intergenic 45.0%
! CAATCATGTCTGCTGCTTCT+TGG - chr8.3:7910737-7910756 None:intergenic 45.0%
! CCTGTAGGTTGAGATTGTGA+TGG + chr8.3:7910650-7910669 MS.gene58950:intron 45.0%
! TGAGATTGTGATGGGACTTG+AGG + chr8.3:7910659-7910678 MS.gene58950:CDS 45.0%
GAATGCTGAGACGGAGAATG+AGG - chr8.3:7908982-7909001 None:intergenic 50.0%
GCAGGGTGAGAAAAGCAAGA+AGG - chr8.3:7910500-7910519 None:intergenic 50.0%
GCTTAAGTGGTTCAAGAGGC+AGG - chr8.3:7910518-7910537 None:intergenic 50.0%
GGAGAATGAGGTTCCTGTGA+TGG - chr8.3:7908970-7908989 None:intergenic 50.0%
GGCAGCTTAAGTGGTTCAAG+AGG - chr8.3:7910522-7910541 None:intergenic 50.0%
GGGTTAGAGAATCTAGCACC+AGG - chr8.3:7909101-7909120 None:intergenic 50.0%
TGCTTCTTGGACGGTGGTAA+TGG - chr8.3:7910724-7910743 None:intergenic 50.0%
TTTGGACTCTCCAGTGACAG+AGG - chr8.3:7909951-7909970 None:intergenic 50.0%
! CATGTCTGCTGCTTCTTGGA+CGG - chr8.3:7910733-7910752 None:intergenic 50.0%
! GATTGTGATGGGACTTGAGG+AGG + chr8.3:7910662-7910681 MS.gene58950:CDS 50.0%
! TGGAGCTGACTCTCTCGATA+CGG + chr8.3:7909982-7910001 MS.gene58950:CDS 50.0%
!! TTGGACGGTGGTAATGGACT+GGG - chr8.3:7910718-7910737 None:intergenic 50.0%
!! CTTGGACGGTGGTAATGGAC+TGG - chr8.3:7910719-7910738 None:intergenic 55.0%
CCAGACGAGTCCTCTGTCAC+TGG + chr8.3:7909938-7909957 MS.gene58950:CDS 60.0%
CCAGTGACAGAGGACTCGTC+TGG - chr8.3:7909941-7909960 None:intergenic 60.0%
! GTCTGCTGCTTCTTGGACGG+TGG - chr8.3:7910730-7910749 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 7908960 7910785 7908960 ID=MS.gene58950
chr8.3 mRNA 7908960 7910785 7908960 ID=MS.gene58950.t1;Parent=MS.gene58950
chr8.3 exon 7908960 7909013 7908960 ID=MS.gene58950.t1.exon1;Parent=MS.gene58950.t1
chr8.3 CDS 7908960 7909013 7908960 ID=cds.MS.gene58950.t1;Parent=MS.gene58950.t1
chr8.3 exon 7909095 7909208 7909095 ID=MS.gene58950.t1.exon2;Parent=MS.gene58950.t1
chr8.3 CDS 7909095 7909208 7909095 ID=cds.MS.gene58950.t1;Parent=MS.gene58950.t1
chr8.3 exon 7909881 7910003 7909881 ID=MS.gene58950.t1.exon3;Parent=MS.gene58950.t1
chr8.3 CDS 7909881 7910003 7909881 ID=cds.MS.gene58950.t1;Parent=MS.gene58950.t1
chr8.3 exon 7910657 7910785 7910657 ID=MS.gene58950.t1.exon4;Parent=MS.gene58950.t1
chr8.3 CDS 7910657 7910785 7910657 ID=cds.MS.gene58950.t1;Parent=MS.gene58950.t1
Gene Sequence

>MS.gene58950

ATGGCATCCATCACAGGAACCTCATTCTCCGTCTCAGCATTCAAACAATCTCTGGTAACAAACAATTCTATTCATTATGGTTTAATTTGTGGTTTATGATCATAATTAATTCTTATGCTAATTCACATCTAACAGGTACCTGGTGCTAGATTCTCTAACCCAAAATCAGTTTCCCTTTCAATTAAGGGGAATGCCTTTCCATCCATTACATTGCAGTCTAGGGTACCTCGCTTTCAAGTTACATGTGCTGTAAGTTCCTCTAAAACCCTCTTAACTCTTTTACTCCCTATATTTTCTTTTATTTTTGGATTAAATATGTTTTTTTTTCTGTTTTTGTTTTTAGTTATTGAATTTATACCAATTTTTAATTTGATCCATGATGTTTTGTTTTATTCCATGTATGTCTGCAGAACATTTTATTTTTGTCTCTCCAGGGCCATGTATAAACTAAAATTATTAAATTAATATTACGTACATATTATAAGGACCAATTTCAATGTGAACAAATTTAAGCTGAACTACAATAAAACAGCAATGAACAAAAGCAAAAAAGTTGTATATTTGAATTGTTTGGGATCACTTAAATGAAAAAATATATTATTCACTTGTAGTAAACTATATACTTATTTTTATAGTATAATTATAAAATTATAGAAATGTTGCTAGTTCAGAAGAGGAGAATGGATTTGTTGTGGTATCACACATTTTGATTTCTTTCACTCATTGTCTTGATTCAGTTAACTACGAGATTTTAATTTCTTCTAATAAAAATTGATTTATTGATTGAACAATATATTTGATGTTGGAATTATCAAGGATTGCATCTACCTCTGATATTTGATTCTGTGTGGTATTTATTTCGACTATATTTGCATTATCTGTTGTGATATAAACGCCTTGGATTTTTACTATTAATATCAGGCGAAAGCAGAAACTGTAAAGAAGGTCTGTGACATTGTCAAAAAACAATTGGCATTGCCAGACGAGTCCTCTGTCACTGGAGAGTCCAAATTTTCTGCACTTGGAGCTGACTCTCTCGATACGGTATTGTCATATTCATAAATTTTGTCTGTGAATTGTTGTCTTTGCATAAAAGTGTTACTCATGCATTCATGATTCACAACACTTGATTGTATTGTATCTATTGCTATTTTATTGCCTTATCTAAGTGTGTGGTCATGTACTTCTTGCTTTCTGGGCTCTAATGCTATCTTGCTGTTTTTGGTGATTTGTTTTCACTTGTCAGAAATACTCGATCCATTTCTCATTAGGTTGTTATTTCATTCTATCTGTTTGTTTCATGTTTAAAATCAGAAGACAGTTATGGCGTTTTTATGCACTTTTGCTGCCAATTTTGAACATCTTTTTCTAGGCTATTAACTGTCCTTTAGTATATTGCTGTGGCAAATGCATACACATGTAATGATATAACACTGTTGACTAATTTAATTTTATTCCCTTTTCTATTTAGAATATCATTTTGACATTATGTTTTTTAATCTACATGGAAAATTGTAATTAAAATGTATTTTGAGTCCCTTCTTGCTTTTCTCACCCTGCCTCTTGAACCACTTAAGCTGCCTAAGTGGAAACTTCAGCATGATTCTACATTTGAGTAAAATGATAACATAGCCTCTGTTGTTTATCTTTTCTAATATTTTCTCTTAACTTATTTAGACTTGTCAAATTTCCTGTAGGTTGAGATTGTGATGGGACTTGAGGAGGAATTTGGTATTAGTGTTGAAGAAGAAAGTGCCCAGTCCATTACCACCGTCCAAGAAGCAGCAGACATGATTGATAATCTTCTTGAAAACAAGAGTGCTTAA

Protein sequence

>MS.gene58950.t1

MASITGTSFSVSAFKQSLVPGARFSNPKSVSLSIKGNAFPSITLQSRVPRFQVTCAAKAETVKKVCDIVKKQLALPDESSVTGESKFSALGADSLDTVEIVMGLEEEFGISVEEESAQSITTVQEAADMIDNLLENKSA