Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59134.t1 | AFK38604.1 | 99.3 | 152 | 1 | 0 | 8 | 159 | 22 | 173 | 1.30E-83 | 318.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59134.t1 | Q9C6Z2 | 76.5 | 153 | 34 | 1 | 9 | 159 | 23 | 175 | 1.5e-67 | 256.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59134.t1 | G7I948 | 99.3 | 152 | 1 | 0 | 8 | 159 | 22 | 173 | 9.4e-84 | 318.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene59134 | 0.806554 | 7.78E-50 | -1.69E-46 |
| MS.gene049395 | MS.gene59134 | 0.81268 | 3.75E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene59134 | 0.842657 | 2.26E-58 | -1.69E-46 |
| MS.gene049887 | MS.gene59134 | 0.824826 | 6.54E-54 | -1.69E-46 |
| MS.gene050838 | MS.gene59134 | 0.813114 | 3.01E-51 | -1.69E-46 |
| MS.gene051234 | MS.gene59134 | 0.827836 | 1.26E-54 | -1.69E-46 |
| MS.gene051885 | MS.gene59134 | 0.820066 | 8.34E-53 | -1.69E-46 |
| MS.gene052072 | MS.gene59134 | 0.807653 | 4.55E-50 | -1.69E-46 |
| MS.gene053143 | MS.gene59134 | 0.828111 | 1.08E-54 | -1.69E-46 |
| MS.gene053144 | MS.gene59134 | 0.805505 | 1.29E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene59134 | 0.817681 | 2.91E-52 | -1.69E-46 |
| MS.gene055891 | MS.gene59134 | 0.81048 | 1.13E-50 | -1.69E-46 |
| MS.gene056043 | MS.gene59134 | -0.802966 | 4.37E-49 | -1.69E-46 |
| MS.gene056881 | MS.gene59134 | 0.810638 | 1.04E-50 | -1.69E-46 |
| MS.gene058149 | MS.gene59134 | 0.813677 | 2.27E-51 | -1.69E-46 |
| MS.gene059778 | MS.gene59134 | 0.814503 | 1.49E-51 | -1.69E-46 |
| MS.gene061100 | MS.gene59134 | 0.828084 | 1.09E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59134.t1 | MTR_1g031150 | 99.342 | 152 | 1 | 0 | 8 | 159 | 22 | 173 | 2.09e-109 | 308 |
| MS.gene59134.t1 | MTR_1g031150 | 93.130 | 131 | 8 | 1 | 8 | 138 | 22 | 151 | 1.30e-83 | 243 |
| MS.gene59134.t1 | MTR_1g090550 | 59.286 | 140 | 56 | 1 | 8 | 146 | 63 | 202 | 1.57e-59 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59134.t1 | AT1G30110 | 76.471 | 153 | 34 | 1 | 9 | 159 | 23 | 175 | 1.94e-85 | 248 |
| MS.gene59134.t1 | AT1G30110 | 76.471 | 153 | 34 | 1 | 9 | 159 | 23 | 175 | 1.94e-85 | 248 |
| MS.gene59134.t1 | AT3G10620 | 56.429 | 140 | 60 | 1 | 8 | 146 | 77 | 216 | 1.60e-56 | 176 |
| MS.gene59134.t1 | AT5G06340 | 57.554 | 139 | 58 | 1 | 8 | 145 | 76 | 214 | 1.22e-53 | 169 |
Find 39 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTCCACTCTGCAAATTC+TGG | 0.093641 | 1.4:+15747609 | None:intergenic |
| TTCTCTAATAGCAGCAGATT+TGG | 0.244965 | 1.4:+15748405 | None:intergenic |
| CAGAATTTGCAGAGTGGAAA+TGG | 0.298244 | 1.4:-15747608 | MS.gene59134:CDS |
| GGCTAAAGTCAATCGTCTTT+GGG | 0.307296 | 1.4:-15748033 | MS.gene59134:CDS |
| ACCTGCTCAATAACTTCTTC+AGG | 0.335949 | 1.4:+15747579 | None:intergenic |
| AGGCTAAAGTCAATCGTCTT+TGG | 0.336576 | 1.4:-15748034 | MS.gene59134:CDS |
| GTTGCATTTCCTTGAAAGTA+AGG | 0.354258 | 1.4:+15747423 | None:intergenic |
| ACCTTCAAGCCTTACTTTCA+AGG | 0.360232 | 1.4:-15747432 | MS.gene59134:CDS |
| GTGCATTACAGGTTGATAAA+TGG | 0.375642 | 1.4:-15748085 | MS.gene59134:intron |
| TCTCTAATAGCAGCAGATTT+GGG | 0.380677 | 1.4:+15748406 | None:intergenic |
| GCTTCCAGATTGAATGTTCC+AGG | 0.385919 | 1.4:-15748593 | MS.gene59134:CDS |
| ATTTCCACTCTGCAAATTCT+GGG | 0.388442 | 1.4:+15747610 | None:intergenic |
| GGTTGTTACAACTTCTTCAT+AGG | 0.389048 | 1.4:+15747452 | None:intergenic |
| AGAGAACTTCGAGAAGAAAC+TGG | 0.413791 | 1.4:-15748388 | MS.gene59134:CDS |
| TCCTTGAAAGTAAGGCTTGA+AGG | 0.425267 | 1.4:+15747431 | None:intergenic |
| TGAGGCATTTGCCATGCTCC+TGG | 0.426678 | 1.4:+15748575 | None:intergenic |
| AAATTCTGGGTCTGCTTCAC+CGG | 0.426680 | 1.4:+15747623 | None:intergenic |
| TGCTCCTGGAACATTCAATC+TGG | 0.446702 | 1.4:+15748589 | None:intergenic |
| AGGAGCATGGCAAATGCCTC+AGG | 0.465802 | 1.4:-15748573 | MS.gene59134:intron |
| CCTGCTCAATAACTTCTTCA+GGG | 0.490006 | 1.4:+15747580 | None:intergenic |
| CCCTGAAGAAGTTATTGAGC+AGG | 0.490394 | 1.4:-15747580 | MS.gene59134:intron |
| GGCATGGACAGGCACAGAAA+TGG | 0.494440 | 1.4:-15748001 | MS.gene59134:intron |
| AGAATTTGCAGAGTGGAAAT+GGG | 0.503598 | 1.4:-15747607 | MS.gene59134:CDS |
| GTTACAACTTCTTCATAGGT+AGG | 0.511880 | 1.4:+15747456 | None:intergenic |
| GGGAGGAGAATGGCATGGAC+AGG | 0.513397 | 1.4:-15748012 | MS.gene59134:CDS |
| ATCGTCTTTGGGGAGGAGAA+TGG | 0.528661 | 1.4:-15748022 | MS.gene59134:CDS |
| TTGACTTTAGCCTTCACAGC+AGG | 0.532701 | 1.4:+15748044 | None:intergenic |
| TGACTTCCCTCCTGCTGTGA+AGG | 0.542301 | 1.4:-15748054 | MS.gene59134:CDS |
| ACTTTAGCCTTCACAGCAGG+AGG | 0.558265 | 1.4:+15748047 | None:intergenic |
| GATTGAATGTTCCAGGAGCA+TGG | 0.573208 | 1.4:-15748586 | MS.gene59134:CDS |
| TTCTGGGTCTGCTTCACCGG+TGG | 0.576581 | 1.4:+15747626 | None:intergenic |
| GCTAAAGTCAATCGTCTTTG+GGG | 0.604526 | 1.4:-15748032 | MS.gene59134:CDS |
| CGAAATGTAAATCGACAAAG+TGG | 0.620980 | 1.4:-15747395 | MS.gene59134:CDS |
| CTTTGGGGAGGAGAATGGCA+TGG | 0.624852 | 1.4:-15748017 | MS.gene59134:CDS |
| GGTGAAATCAACTTAGCCAC+CGG | 0.634355 | 1.4:-15747642 | MS.gene59134:CDS |
| CTTTAGCCTTCACAGCAGGA+GGG | 0.649727 | 1.4:+15748048 | None:intergenic |
| AAAGTCAATCGTCTTTGGGG+AGG | 0.655166 | 1.4:-15748029 | MS.gene59134:CDS |
| GTCTGCTGAGATAATCGCTG+AGG | 0.670608 | 1.4:-15748359 | MS.gene59134:intron |
| CAGACCCAGAATTTGCAGAG+TGG | 0.693037 | 1.4:-15747614 | MS.gene59134:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTTAAGTTATTTGAAT+TGG | + | chr1.4:15747509-15747528 | None:intergenic | 10.0% |
| !! | ATGAAATTAAAAAAAGATGA+CGG | - | chr1.4:15748355-15748374 | MS.gene59134:intron | 15.0% |
| !!! | ATATTTTTTCGAGTAAAATT+TGG | - | chr1.4:15748215-15748234 | MS.gene59134:intron | 15.0% |
| !! | ATCAATTACGAACATTTAAT+AGG | - | chr1.4:15748326-15748345 | MS.gene59134:intron | 20.0% |
| !! | GAAAAATACATAATTGTTAC+AGG | + | chr1.4:15748482-15748501 | None:intergenic | 20.0% |
| !!! | AATTTTTTGACTGTTCTTTT+GGG | - | chr1.4:15747557-15747576 | MS.gene59134:intron | 20.0% |
| !!! | ACAAAAGCATATATTAACAA+TGG | - | chr1.4:15748099-15748118 | MS.gene59134:intron | 20.0% |
| !!! | TCTTTTGTTATTAATAAGCA+TGG | - | chr1.4:15748254-15748273 | MS.gene59134:intron | 20.0% |
| !!! | TTTGTTTTTTCAGATATTTG+TGG | - | chr1.4:15747403-15747422 | MS.gene59134:CDS | 20.0% |
| ! | AACTAAATGCAGAAAAGAAA+AGG | + | chr1.4:15747696-15747715 | None:intergenic | 25.0% |
| ! | AAGAGAAACATTTAAGAATG+TGG | + | chr1.4:15748077-15748096 | None:intergenic | 25.0% |
| ! | ACAAAGTCAGTATTTCTTAT+TGG | - | chr1.4:15748291-15748310 | MS.gene59134:intron | 25.0% |
| ! | TGTTTGAAAAACTAATCACT+GGG | - | chr1.4:15748504-15748523 | MS.gene59134:intron | 25.0% |
| !!! | ATTTTTTGACTGTTCTTTTG+GGG | - | chr1.4:15747558-15747577 | MS.gene59134:intron | 25.0% |
| !!! | CAATTTTTTGACTGTTCTTT+TGG | - | chr1.4:15747556-15747575 | MS.gene59134:intron | 25.0% |
| !!! | TTTGTTATTAATAAGCATGG+TGG | - | chr1.4:15748257-15748276 | MS.gene59134:intron | 25.0% |
| AGAACAGTCAAAAAATTGCT+CGG | + | chr1.4:15747554-15747573 | None:intergenic | 30.0% | |
| CTGTTTGAAAAACTAATCAC+TGG | - | chr1.4:15748503-15748522 | MS.gene59134:intron | 30.0% | |
| GTATACTAGTACATAATGTG+AGG | + | chr1.4:15747474-15747493 | None:intergenic | 30.0% | |
| !! | AGAAATACTGACTTTGTTAG+CGG | + | chr1.4:15748288-15748307 | None:intergenic | 30.0% |
| AGAATTTGCAGAGTGGAAAT+GGG | - | chr1.4:15748411-15748430 | MS.gene59134:CDS | 35.0% | |
| ATCAATACTCCATAGAGCAT+CGG | + | chr1.4:15747757-15747776 | None:intergenic | 35.0% | |
| ATTTCCACTCTGCAAATTCT+GGG | + | chr1.4:15748411-15748430 | None:intergenic | 35.0% | |
| CGAAAAAATATCACGAGACA+AGG | + | chr1.4:15748206-15748225 | None:intergenic | 35.0% | |
| CGAAATGTAAATCGACAAAG+TGG | - | chr1.4:15748623-15748642 | MS.gene59134:CDS | 35.0% | |
| GGTTGTTACAACTTCTTCAT+AGG | + | chr1.4:15748569-15748588 | None:intergenic | 35.0% | |
| GTTACAACTTCTTCATAGGT+AGG | + | chr1.4:15748565-15748584 | None:intergenic | 35.0% | |
| GTTGCATTTCCTTGAAAGTA+AGG | + | chr1.4:15748598-15748617 | None:intergenic | 35.0% | |
| TCTCTAATAGCAGCAGATTT+GGG | + | chr1.4:15747615-15747634 | None:intergenic | 35.0% | |
| TGCATCAATGAAAGCGATAT+AGG | - | chr1.4:15748147-15748166 | MS.gene59134:intron | 35.0% | |
| TTCTCTAATAGCAGCAGATT+TGG | + | chr1.4:15747616-15747635 | None:intergenic | 35.0% | |
| !! | GTGCATTACAGGTTGATAAA+TGG | - | chr1.4:15747933-15747952 | MS.gene59134:intron | 35.0% |
| !!! | ACTGTTCTTTTGGGGTTTTT+AGG | - | chr1.4:15747566-15747585 | MS.gene59134:intron | 35.0% |
| !!! | CTGTTCTTTTGGGGTTTTTA+GGG | - | chr1.4:15747567-15747586 | MS.gene59134:intron | 35.0% |
| !!! | TTTTAGGGAGGTATTGAAGA+TGG | - | chr1.4:15747582-15747601 | MS.gene59134:CDS | 35.0% |
| ACCTGCTCAATAACTTCTTC+AGG | + | chr1.4:15748442-15748461 | None:intergenic | 40.0% | |
| ACCTTCAAGCCTTACTTTCA+AGG | - | chr1.4:15748586-15748605 | MS.gene59134:CDS | 40.0% | |
| AGAGAACTTCGAGAAGAAAC+TGG | - | chr1.4:15747630-15747649 | MS.gene59134:CDS | 40.0% | |
| AGGCTAAAGTCAATCGTCTT+TGG | - | chr1.4:15747984-15748003 | MS.gene59134:intron | 40.0% | |
| ATTTCTCTGCGATAACTCTG+TGG | - | chr1.4:15747878-15747897 | MS.gene59134:intron | 40.0% | |
| CAGAATTTGCAGAGTGGAAA+TGG | - | chr1.4:15748410-15748429 | MS.gene59134:CDS | 40.0% | |
| CATTTCCACTCTGCAAATTC+TGG | + | chr1.4:15748412-15748431 | None:intergenic | 40.0% | |
| CCTGCTCAATAACTTCTTCA+GGG | + | chr1.4:15748441-15748460 | None:intergenic | 40.0% | |
| CTGGGCTAATAATCGATTAC+AGG | - | chr1.4:15748522-15748541 | MS.gene59134:intron | 40.0% | |
| GCTAAAGTCAATCGTCTTTG+GGG | - | chr1.4:15747986-15748005 | MS.gene59134:intron | 40.0% | |
| GGCTAAAGTCAATCGTCTTT+GGG | - | chr1.4:15747985-15748004 | MS.gene59134:intron | 40.0% | |
| GGCTAATAATCGATTACAGG+CGG | - | chr1.4:15748525-15748544 | MS.gene59134:intron | 40.0% | |
| TAATAATCGATTACAGGCGG+TGG | - | chr1.4:15748528-15748547 | MS.gene59134:intron | 40.0% | |
| TCCTTGAAAGTAAGGCTTGA+AGG | + | chr1.4:15748590-15748609 | None:intergenic | 40.0% | |
| TGACAGTGTTTGTGCATTAC+AGG | - | chr1.4:15747922-15747941 | MS.gene59134:intron | 40.0% | |
| !!! | TTCTTTTGGGGTTTTTAGGG+AGG | - | chr1.4:15747570-15747589 | MS.gene59134:intron | 40.0% |
| AAATTCTGGGTCTGCTTCAC+CGG | + | chr1.4:15748398-15748417 | None:intergenic | 45.0% | |
| ACATAATGTGAGGTCACCTG+AGG | + | chr1.4:15747464-15747483 | None:intergenic | 45.0% | |
| CATTTACTCCATGCACACTC+AGG | + | chr1.4:15748176-15748195 | None:intergenic | 45.0% | |
| CCCTGAAGAAGTTATTGAGC+AGG | - | chr1.4:15748438-15748457 | MS.gene59134:intron | 45.0% | |
| CTGAGTGTGCATGGAGTAAA+TGG | - | chr1.4:15748174-15748193 | MS.gene59134:intron | 45.0% | |
| GATTGAATGTTCCAGGAGCA+TGG | - | chr1.4:15747432-15747451 | MS.gene59134:CDS | 45.0% | |
| GCTTCCAGATTGAATGTTCC+AGG | - | chr1.4:15747425-15747444 | MS.gene59134:CDS | 45.0% | |
| GGTGAAATCAACTTAGCCAC+CGG | - | chr1.4:15748376-15748395 | MS.gene59134:CDS | 45.0% | |
| TGCTCCTGGAACATTCAATC+TGG | + | chr1.4:15747432-15747451 | None:intergenic | 45.0% | |
| ! | AAAGTCAATCGTCTTTGGGG+AGG | - | chr1.4:15747989-15748008 | MS.gene59134:intron | 45.0% |
| ! | TTGACTTTAGCCTTCACAGC+AGG | + | chr1.4:15747977-15747996 | None:intergenic | 45.0% |
| ATAGGAAGCCTGAGTGTGCA+TGG | - | chr1.4:15748165-15748184 | MS.gene59134:intron | 50.0% | |
| CAGACCCAGAATTTGCAGAG+TGG | - | chr1.4:15748404-15748423 | MS.gene59134:CDS | 50.0% | |
| GTCTGCTGAGATAATCGCTG+AGG | - | chr1.4:15747659-15747678 | MS.gene59134:CDS | 50.0% | |
| TACGTCTAGCCGATGCTCTA+TGG | - | chr1.4:15747745-15747764 | MS.gene59134:intron | 50.0% | |
| ! | ACTTTAGCCTTCACAGCAGG+AGG | + | chr1.4:15747974-15747993 | None:intergenic | 50.0% |
| ! | ATCGTCTTTGGGGAGGAGAA+TGG | - | chr1.4:15747996-15748015 | MS.gene59134:intron | 50.0% |
| ! | CTTTAGCCTTCACAGCAGGA+GGG | + | chr1.4:15747973-15747992 | None:intergenic | 50.0% |
| GGCATGGACAGGCACAGAAA+TGG | - | chr1.4:15748017-15748036 | MS.gene59134:CDS | 55.0% | |
| TGACTTCCCTCCTGCTGTGA+AGG | - | chr1.4:15747964-15747983 | MS.gene59134:intron | 55.0% | |
| ! | AGGAGCATGGCAAATGCCTC+AGG | - | chr1.4:15747445-15747464 | MS.gene59134:CDS | 55.0% |
| ! | CTTTGGGGAGGAGAATGGCA+TGG | - | chr1.4:15748001-15748020 | MS.gene59134:intron | 55.0% |
| ! | TGAGGCATTTGCCATGCTCC+TGG | + | chr1.4:15747446-15747465 | None:intergenic | 55.0% |
| GGGAGGAGAATGGCATGGAC+AGG | - | chr1.4:15748006-15748025 | MS.gene59134:CDS | 60.0% | |
| TTCTGGGTCTGCTTCACCGG+TGG | + | chr1.4:15748395-15748414 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 15747392 | 15748648 | 15747392 | ID=MS.gene59134 |
| chr1.4 | mRNA | 15747392 | 15748648 | 15747392 | ID=MS.gene59134.t1;Parent=MS.gene59134 |
| chr1.4 | exon | 15748574 | 15748648 | 15748574 | ID=MS.gene59134.t1.exon1;Parent=MS.gene59134.t1 |
| chr1.4 | CDS | 15748574 | 15748648 | 15748574 | ID=cds.MS.gene59134.t1;Parent=MS.gene59134.t1 |
| chr1.4 | exon | 15748360 | 15748452 | 15748360 | ID=MS.gene59134.t1.exon2;Parent=MS.gene59134.t1 |
| chr1.4 | CDS | 15748360 | 15748452 | 15748360 | ID=cds.MS.gene59134.t1;Parent=MS.gene59134.t1 |
| chr1.4 | exon | 15748002 | 15748096 | 15748002 | ID=MS.gene59134.t1.exon3;Parent=MS.gene59134.t1 |
| chr1.4 | CDS | 15748002 | 15748096 | 15748002 | ID=cds.MS.gene59134.t1;Parent=MS.gene59134.t1 |
| chr1.4 | exon | 15747581 | 15747692 | 15747581 | ID=MS.gene59134.t1.exon4;Parent=MS.gene59134.t1 |
| chr1.4 | CDS | 15747581 | 15747692 | 15747581 | ID=cds.MS.gene59134.t1;Parent=MS.gene59134.t1 |
| chr1.4 | exon | 15747392 | 15747496 | 15747392 | ID=MS.gene59134.t1.exon5;Parent=MS.gene59134.t1 |
| chr1.4 | CDS | 15747392 | 15747496 | 15747392 | ID=cds.MS.gene59134.t1;Parent=MS.gene59134.t1 |
| Gene Sequence |
| Protein sequence |
