AlfalfaGEDB | MS.gene60598

M. sativa cultivar XinJiangDaYe / MS.gene60598

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60598.t1	XP_024642361.1	97.1	272	8	0	1	272	1	272	3.50E-145	524.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60598.t1	A0A396JW40	97.1	272	8	0	1	272	1	272	2.5e-145	524.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049397	MS.gene60598	0.807482	4.95E-50	-1.69E-46
MS.gene049596	MS.gene60598	0.829047	6.40E-55	-1.69E-46
MS.gene049989	MS.gene60598	0.814051	1.88E-51	-1.69E-46
MS.gene050297	MS.gene60598	0.894051	3.55E-75	-1.69E-46
MS.gene051478	MS.gene60598	0.809048	2.29E-50	-1.69E-46
MS.gene052367	MS.gene60598	0.808702	2.72E-50	-1.69E-46
MS.gene054643	MS.gene60598	0.829917	3.94E-55	-1.69E-46
MS.gene055764	MS.gene60598	0.806792	6.92E-50	-1.69E-46
MS.gene057483	MS.gene60598	0.818439	1.96E-52	-1.69E-46
MS.gene05870	MS.gene60598	0.803006	4.28E-49	-1.69E-46
MS.gene058844	MS.gene60598	0.802972	4.35E-49	-1.69E-46
MS.gene059300	MS.gene60598	0.824312	8.64E-54	-1.69E-46
MS.gene06057	MS.gene60598	0.851827	6.89E-61	-1.69E-46
MS.gene061260	MS.gene60598	0.810113	1.35E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 50 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACATGATCAATGACATTT+TGG	0.223671	1.2:-69476290	None:intergenic
ATTGAAATCCTTGAAAATTC+TGG	0.276980	1.2:-69477261	None:intergenic
AACAATAGTGGGGTTGTTCT+TGG	0.286826	1.2:-69476590	None:intergenic
TCTGGACTCTTGCAGCATAC+TGG	0.312031	1.2:-69477243	None:intergenic
TCAGTCTGATGATGCATTCC+TGG	0.314010	1.2:+69477158	MS.gene60598:intron
AATGTCATTGATCATGTTAA+TGG	0.315572	1.2:+69476294	MS.gene60598:CDS
CAGGCATCTCCTTCAATTCC+AGG	0.329164	1.2:-69477176	None:intergenic
GCAGATGGAGACGGCATATC+AGG	0.349775	1.2:-69477207	None:intergenic
TTGCAGGAAAGATGAAATCT+AGG	0.354095	1.2:+69476437	MS.gene60598:CDS
TTTCCATCTACTATATCAAT+AGG	0.355572	1.2:-69476505	None:intergenic
TTCTTGGACTTGAGTAGATT+TGG	0.362992	1.2:-69476574	None:intergenic
CGCTCTTCGGCAATTGCATC+AGG	0.377764	1.2:-69476811	None:intergenic
GGCATATCAGGCAACTCTTC+AGG	0.388449	1.2:-69477195	None:intergenic
GTGAAATTGCAATCGAAAGC+TGG	0.396566	1.2:-69477294	None:intergenic
TTTAATTGTGCTACCATTGA+AGG	0.397307	1.2:-69476533	None:intergenic
ATCCTTATGTAGAACAATAG+TGG	0.407861	1.2:-69476602	None:intergenic
CTGGACTCTTGCAGCATACT+GGG	0.413396	1.2:-69477242	None:intergenic
AAGCCTATTGATATAGTAGA+TGG	0.426272	1.2:+69476502	MS.gene60598:CDS
TCCTTATGTAGAACAATAGT+GGG	0.435174	1.2:-69476601	None:intergenic
GCTTCTAAGAAGTCAAATAC+TGG	0.435698	1.2:+69476685	MS.gene60598:CDS
ATCAATAGGCTTCACTGCAT+TGG	0.447906	1.2:-69476491	None:intergenic
CTTGAAATCAACAAAAGAAC+TGG	0.448659	1.2:+69476655	MS.gene60598:CDS
CCCCACTATTGTTCTACATA+AGG	0.466740	1.2:+69476600	MS.gene60598:CDS
ACCTTCAGTTTGCTAAAAGC+AGG	0.486566	1.2:-69477049	None:intergenic
TGATGCATTCCTGGAATTGA+AGG	0.486995	1.2:+69477167	MS.gene60598:CDS
ATTATGAAACATCCGCTCTT+CGG	0.490772	1.2:-69476824	None:intergenic
AAAATCACAAAGTCATGCAT+TGG	0.496042	1.2:+69476861	MS.gene60598:CDS
TATTGTTCTACATAAGGATA+AGG	0.500681	1.2:+69476606	MS.gene60598:CDS
GATGGAAAGAAGGCCTTCAA+TGG	0.509301	1.2:+69476520	MS.gene60598:CDS
TGCAGGAAAGATGAAATCTA+GGG	0.516990	1.2:+69476438	MS.gene60598:CDS
TTTGGTGTTTGATTGACAGA+GGG	0.545419	1.2:-69476556	None:intergenic
TAAGGAAGTTGTTTCTGCCA+AGG	0.545987	1.2:+69476624	MS.gene60598:CDS
TGAAATTGCAATCGAAAGCT+GGG	0.549449	1.2:-69477293	None:intergenic
AGAAGTCAAATACTGGCAGC+AGG	0.552216	1.2:+69476692	MS.gene60598:CDS
TGATATAGTAGATGGAAAGA+AGG	0.559608	1.2:+69476510	MS.gene60598:CDS
CTTTAAAAGAACTAGTGTCC+AGG	0.560073	1.2:-69476774	None:intergenic
TTTCAAGACTGCTATACTTG+GGG	0.563438	1.2:+69476897	MS.gene60598:CDS
AAGGAAGTTGTTTCTGCCAA+GGG	0.563895	1.2:+69476625	MS.gene60598:CDS
ATTTGGTGTTTGATTGACAG+AGG	0.569171	1.2:-69476557	None:intergenic
GGAAAGATGAAATCTAGGGC+TGG	0.572546	1.2:+69476442	MS.gene60598:CDS
GGGGAACCATGCTTAGCAGA+TGG	0.575621	1.2:-69477222	None:intergenic
CCGTCTCCATCTGCTAAGCA+TGG	0.588140	1.2:+69477216	MS.gene60598:CDS
AAGACTGCTATACTTGGGGA+TGG	0.598286	1.2:+69476901	MS.gene60598:CDS
CTGATGCAATTGCCGAAGAG+CGG	0.629692	1.2:+69476812	MS.gene60598:CDS
CCATGCTTAGCAGATGGAGA+CGG	0.635945	1.2:-69477216	None:intergenic
AAGATGAAATCTAGGGCTGG+AGG	0.656807	1.2:+69476445	MS.gene60598:CDS
TGGACTCTTGCAGCATACTG+GGG	0.672423	1.2:-69477241	None:intergenic
GCTGGAGGAAGAAAACCACT+TGG	0.686170	1.2:+69476460	MS.gene60598:CDS
CCTTATGTAGAACAATAGTG+GGG	0.709845	1.2:-69476600	None:intergenic
GCATTGGATAGATCACCAAG+TGG	0.747744	1.2:-69476475	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAGTTAAAAAATCAAAAC+AGG	-	chr1.2:69477126-69477145	None:intergenic	15.0%
!!!	GTTTTGATTTTTTAACTATT+TGG	+	chr1.2:69477126-69477145	MS.gene60598:intron	15.0%
!	AATGTCATTGATCATGTTAA+TGG	+	chr1.2:69476294-69476313	MS.gene60598:CDS	25.0%
!	AATTGTTTATGTGTGTAGAA+TGG	+	chr1.2:69476357-69476376	MS.gene60598:intron	25.0%
!	ATACATAACTTGATCAGATT+TGG	+	chr1.2:69476997-69477016	MS.gene60598:intron	25.0%
!	ATTGAAATCCTTGAAAATTC+TGG	-	chr1.2:69477264-69477283	None:intergenic	25.0%
!	TATTGTTCTACATAAGGATA+AGG	+	chr1.2:69476606-69476625	MS.gene60598:CDS	25.0%
!	TGTACCTGATATGAAAAATA+AGG	+	chr1.2:69476747-69476766	MS.gene60598:CDS	25.0%
!	TTTCCATCTACTATATCAAT+AGG	-	chr1.2:69476508-69476527	None:intergenic	25.0%
!!	AGATTCTGTTATGGTAATTT+TGG	+	chr1.2:69476946-69476965	MS.gene60598:intron	25.0%
!!	TAACATGATCAATGACATTT+TGG	-	chr1.2:69476293-69476312	None:intergenic	25.0%
!!	TCATGTTAATGGTATTTTTC+TGG	+	chr1.2:69476305-69476324	MS.gene60598:intron	25.0%
!!!	AATTTTCAAGGATTTCAATG+TGG	+	chr1.2:69477265-69477284	MS.gene60598:CDS	25.0%
!!!	ATTTTCAAGGATTTCAATGT+GGG	+	chr1.2:69477266-69477285	MS.gene60598:CDS	25.0%
	AAAATCACAAAGTCATGCAT+TGG	+	chr1.2:69476861-69476880	MS.gene60598:CDS	30.0%
	AAGCCTATTGATATAGTAGA+TGG	+	chr1.2:69476502-69476521	MS.gene60598:CDS	30.0%
	ATCCTTATGTAGAACAATAG+TGG	-	chr1.2:69476605-69476624	None:intergenic	30.0%
	CTACACACATAAACAATTGA+TGG	-	chr1.2:69476355-69476374	None:intergenic	30.0%
	CTTGAAATCAACAAAAGAAC+TGG	+	chr1.2:69476655-69476674	MS.gene60598:CDS	30.0%
	TCCTTATGTAGAACAATAGT+GGG	-	chr1.2:69476604-69476623	None:intergenic	30.0%
	TGATATAGTAGATGGAAAGA+AGG	+	chr1.2:69476510-69476529	MS.gene60598:CDS	30.0%
	TTTAATTGTGCTACCATTGA+AGG	-	chr1.2:69476536-69476555	None:intergenic	30.0%
!	ACTGATTCTAGATTCTGTTA+TGG	+	chr1.2:69476937-69476956	MS.gene60598:intron	30.0%
!	TCTATAAAGACAGATTTTGC+AGG	+	chr1.2:69476421-69476440	MS.gene60598:CDS	30.0%
!!	TGGACACTAGTTCTTTTAAA+GGG	+	chr1.2:69476776-69476795	MS.gene60598:CDS	30.0%
!!	TTTTCAAGACTGCTATACTT+GGG	+	chr1.2:69476896-69476915	MS.gene60598:CDS	30.0%
!!!	CTGTCCTTATTTTTCATATC+AGG	-	chr1.2:69476754-69476773	None:intergenic	30.0%
!!!	GATTTCAAGATTTTTTCCCT+TGG	-	chr1.2:69476644-69476663	None:intergenic	30.0%
!!!	TTTTTCAAGACTGCTATACT+TGG	+	chr1.2:69476895-69476914	MS.gene60598:CDS	30.0%
	ATTATGAAACATCCGCTCTT+CGG	-	chr1.2:69476827-69476846	None:intergenic	35.0%
	CCTTATGTAGAACAATAGTG+GGG	-	chr1.2:69476603-69476622	None:intergenic	35.0%
	TATGAAAAATAAGGACAGCC+TGG	+	chr1.2:69476756-69476775	MS.gene60598:CDS	35.0%
	TGAAATTGCAATCGAAAGCT+GGG	-	chr1.2:69477296-69477315	None:intergenic	35.0%
	TGCAGGAAAGATGAAATCTA+GGG	+	chr1.2:69476438-69476457	MS.gene60598:CDS	35.0%
	TTCTTGGACTTGAGTAGATT+TGG	-	chr1.2:69476577-69476596	None:intergenic	35.0%
	TTGCAGGAAAGATGAAATCT+AGG	+	chr1.2:69476437-69476456	MS.gene60598:CDS	35.0%
	TTTATGTGTGTAGAATGGCT+AGG	+	chr1.2:69476362-69476381	MS.gene60598:intron	35.0%
	TTTCAAGACTGCTATACTTG+GGG	+	chr1.2:69476897-69476916	MS.gene60598:CDS	35.0%
!	CTTTAAAAGAACTAGTGTCC+AGG	-	chr1.2:69476777-69476796	None:intergenic	35.0%
!	GCTTCTAAGAAGTCAAATAC+TGG	+	chr1.2:69476685-69476704	MS.gene60598:CDS	35.0%
!!	ATTTGGTGTTTGATTGACAG+AGG	-	chr1.2:69476560-69476579	None:intergenic	35.0%
!!	CTGGACACTAGTTCTTTTAA+AGG	+	chr1.2:69476775-69476794	MS.gene60598:CDS	35.0%
!!	TTTGGTGTTTGATTGACAGA+GGG	-	chr1.2:69476559-69476578	None:intergenic	35.0%
	ATCAATAGGCTTCACTGCAT+TGG	-	chr1.2:69476494-69476513	None:intergenic	40.0%
	CATCATCAGACTGAAAAGCA+GGG	-	chr1.2:69477152-69477171	None:intergenic	40.0%
	CCCCACTATTGTTCTACATA+AGG	+	chr1.2:69476600-69476619	MS.gene60598:CDS	40.0%
	GTGAAATTGCAATCGAAAGC+TGG	-	chr1.2:69477297-69477316	None:intergenic	40.0%
	TGATGCATTCCTGGAATTGA+AGG	+	chr1.2:69477167-69477186	MS.gene60598:CDS	40.0%
!	AACAATAGTGGGGTTGTTCT+TGG	-	chr1.2:69476593-69476612	None:intergenic	40.0%
!	GCAAGAGTCCAGAATTTTCA+AGG	+	chr1.2:69477253-69477272	MS.gene60598:CDS	40.0%
!!	AAGGAAGTTGTTTCTGCCAA+GGG	+	chr1.2:69476625-69476644	MS.gene60598:CDS	40.0%
!!	ACCTTCAGTTTGCTAAAAGC+AGG	-	chr1.2:69477052-69477071	None:intergenic	40.0%
!!	TAAGGAAGTTGTTTCTGCCA+AGG	+	chr1.2:69476624-69476643	MS.gene60598:CDS	40.0%
	AAGACTGCTATACTTGGGGA+TGG	+	chr1.2:69476901-69476920	MS.gene60598:CDS	45.0%
	AGAAGTCAAATACTGGCAGC+AGG	+	chr1.2:69476692-69476711	MS.gene60598:CDS	45.0%
	GATGGAAAGAAGGCCTTCAA+TGG	+	chr1.2:69476520-69476539	MS.gene60598:CDS	45.0%
	GCATCATCAGACTGAAAAGC+AGG	-	chr1.2:69477153-69477172	None:intergenic	45.0%
	GCATTGGATAGATCACCAAG+TGG	-	chr1.2:69476478-69476497	None:intergenic	45.0%
!	AAGATGAAATCTAGGGCTGG+AGG	+	chr1.2:69476445-69476464	MS.gene60598:CDS	45.0%
!	GCTAGGCTGATTCTTAATGC+TGG	+	chr1.2:69476379-69476398	MS.gene60598:intron	45.0%
!	GGAAAGATGAAATCTAGGGC+TGG	+	chr1.2:69476442-69476461	MS.gene60598:CDS	45.0%
!	TCAGTCTGATGATGCATTCC+TGG	+	chr1.2:69477158-69477177	MS.gene60598:intron	45.0%
!!	GCCTGCTTTTAGCAAACTGA+AGG	+	chr1.2:69477048-69477067	MS.gene60598:CDS	45.0%
	CAGGCATCTCCTTCAATTCC+AGG	-	chr1.2:69477179-69477198	None:intergenic	50.0%
	CCATGCTTAGCAGATGGAGA+CGG	-	chr1.2:69477219-69477238	None:intergenic	50.0%
	CTGATGCAATTGCCGAAGAG+CGG	+	chr1.2:69476812-69476831	MS.gene60598:CDS	50.0%
	CTGGACTCTTGCAGCATACT+GGG	-	chr1.2:69477245-69477264	None:intergenic	50.0%
	CTTAATGCTGGTGCAGATGC+AGG	+	chr1.2:69476391-69476410	MS.gene60598:intron	50.0%
	GCTGGAGGAAGAAAACCACT+TGG	+	chr1.2:69476460-69476479	MS.gene60598:CDS	50.0%
	GGCATATCAGGCAACTCTTC+AGG	-	chr1.2:69477198-69477217	None:intergenic	50.0%
	TCTGGACTCTTGCAGCATAC+TGG	-	chr1.2:69477246-69477265	None:intergenic	50.0%
	TGGACTCTTGCAGCATACTG+GGG	-	chr1.2:69477244-69477263	None:intergenic	50.0%
	CCGTCTCCATCTGCTAAGCA+TGG	+	chr1.2:69477216-69477235	MS.gene60598:CDS	55.0%
	CGCTCTTCGGCAATTGCATC+AGG	-	chr1.2:69476814-69476833	None:intergenic	55.0%
	GCAGATGGAGACGGCATATC+AGG	-	chr1.2:69477210-69477229	None:intergenic	55.0%
	GGGGAACCATGCTTAGCAGA+TGG	-	chr1.2:69477225-69477244	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	69476258	69477350	69476258	ID=MS.gene60598
chr1.2	mRNA	69476258	69477350	69476258	ID=MS.gene60598.t1;Parent=MS.gene60598
chr1.2	exon	69476258	69476315	69476258	ID=MS.gene60598.t1.exon1;Parent=MS.gene60598.t1
chr1.2	CDS	69476258	69476315	69476258	ID=cds.MS.gene60598.t1;Parent=MS.gene60598.t1
chr1.2	exon	69476407	69476922	69476407	ID=MS.gene60598.t1.exon2;Parent=MS.gene60598.t1
chr1.2	CDS	69476407	69476922	69476407	ID=cds.MS.gene60598.t1;Parent=MS.gene60598.t1
chr1.2	exon	69477014	69477069	69477014	ID=MS.gene60598.t1.exon3;Parent=MS.gene60598.t1
chr1.2	CDS	69477014	69477069	69477014	ID=cds.MS.gene60598.t1;Parent=MS.gene60598.t1
chr1.2	exon	69477162	69477350	69477162	ID=MS.gene60598.t1.exon4;Parent=MS.gene60598.t1
chr1.2	CDS	69477162	69477350	69477162	ID=cds.MS.gene60598.t1;Parent=MS.gene60598.t1

Gene Sequence

>MS.gene60598

ATGGCTGCACGAACTGTTCGTTTGTTTCAAGACCAAAATGTCATTGATCATGTTAATGGTATTTTTCTGGTTCATTAAGTTTGAACATGTTTTGCCATCAATTGTTTATGTGTGTAGAATGGCTAGGCTGATTCTTAATGCTGGTGCAGATGCAGGAACTATGTCTATAAAGACAGATTTTGCAGGAAAGATGAAATCTAGGGCTGGAGGAAGAAAACCACTTGGTGATCTATCCAATGCAGTGAAGCCTATTGATATAGTAGATGGAAAGAAGGCCTTCAATGGTAGCACAATTAAACCCTCTGTCAATCAAACACCAAATCTACTCAAGTCCAAGAACAACCCCACTATTGTTCTACATAAGGATAAGGAAGTTGTTTCTGCCAAGGGAAAAAATCTTGAAATCAACAAAAGAACTGGCAGCAAAGCTTCTAAGAAGTCAAATACTGGCAGCAGGAGAGCACTTACTGACATCTCAAACTCGCTGCATGTACCTGATATGAAAAATAAGGACAGCCTGGACACTAGTTCTTTTAAAGGGAAATATCTTCATCCTGATGCAATTGCCGAAGAGCGGATGTTTCATAATCATGAAGAATGTATAAAATCACAAAGTCATGCATTGGATATGCATCATTTTTTCAAGACTGCTATACTTGGGGATGGTAATTAGTTATTTACTGATTCTAGATTCTGTTATGGTAATTTTGGCTGATCACTTTTACTTATTAATTGATTTATACATAACTTGATCAGATTTGGATGATGACATGAAAATTTCATTTGAACAGCCTGCTTTTAGCAAACTGAAGGTTCGTCTAAAACTACTTTCATTTCTCATGTGTGTTTCTCATGTGTTGTTTATCCTGTTTTGATTTTTTAACTATTTGGCCCTGCTTTTCAGTCTGATGATGCATTCCTGGAATTGAAGGAGATGCCTGAAGAGTTGCCTGATATGCCGTCTCCATCTGCTAAGCATGGTTCCCCAGTATGCTGCAAGAGTCCAGAATTTTCAAGGATTTCAATGTGGGATGACCCAGCTTTCGATTGCAATTTCACTTTGATAGAATCACCCAAATCGTCTAAAAACTGA

Protein sequence

>MS.gene60598.t1

MAARTVRLFQDQNVIDHVNDAGTMSIKTDFAGKMKSRAGGRKPLGDLSNAVKPIDIVDGKKAFNGSTIKPSVNQTPNLLKSKNNPTIVLHKDKEVVSAKGKNLEINKRTGSKASKKSNTGSRRALTDISNSLHVPDMKNKDSLDTSSFKGKYLHPDAIAEERMFHNHEECIKSQSHALDMHHFFKTAILGDDLDDDMKISFEQPAFSKLKSDDAFLELKEMPEELPDMPSPSAKHGSPVCCKSPEFSRISMWDDPAFDCNFTLIESPKSSKN