AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60930.t1 MTR_2g063080 97.468 553 13 1 1 553 1 552 0.0 1089
MS.gene60930.t1 MTR_2g100230 29.372 446 256 9 86 526 69 460 8.38e-49 176
MS.gene60930.t1 MTR_7g056830 30.073 409 227 7 108 515 9 359 5.25e-47 170
MS.gene60930.t1 MTR_6g082080 30.732 410 223 8 108 515 9 359 2.69e-46 168
MS.gene60930.t1 MTR_6g005550 29.358 436 250 9 95 526 147 528 2.87e-44 167
MS.gene60930.t1 MTR_3g449250 29.138 429 241 11 110 536 91 458 6.35e-44 164
MS.gene60930.t1 MTR_3g449250 29.138 429 241 11 110 536 91 458 7.14e-44 164
MS.gene60930.t1 MTR_2g438110 25.917 436 257 8 106 535 31 406 1.01e-43 160
MS.gene60930.t1 MTR_2g043360 26.608 451 289 10 105 524 37 476 7.22e-43 162
MS.gene60930.t1 MTR_7g109720 27.869 488 271 13 49 515 159 586 7.73e-43 164
MS.gene60930.t1 MTR_4g059460 25.991 454 294 10 102 524 37 479 1.45e-42 161
MS.gene60930.t1 MTR_5g090120 28.205 429 245 11 110 536 82 449 2.79e-42 160
MS.gene60930.t1 MTR_4g134170 27.623 467 277 10 67 525 255 668 4.52e-42 161
MS.gene60930.t1 MTR_1g095810 27.765 425 238 11 101 514 137 503 1.36e-41 158
MS.gene60930.t1 MTR_1g026830 27.316 421 245 9 101 514 139 505 1.93e-41 157
MS.gene60930.t1 MTR_4g079320 27.103 428 247 9 110 532 20 387 2.06e-41 159
MS.gene60930.t1 MTR_2g039960 26.118 425 247 8 109 529 41 402 6.47e-41 153
MS.gene60930.t1 MTR_8g018400 25.943 424 249 8 109 529 41 402 4.65e-40 150
MS.gene60930.t1 MTR_6g005320 27.571 457 268 11 77 525 227 628 7.46e-40 154
MS.gene60930.t1 MTR_4g125970 25.776 419 244 8 109 523 41 396 1.23e-39 149
MS.gene60930.t1 MTR_2g099620 25.952 420 244 9 108 523 24 380 1.66e-39 149
MS.gene60930.t1 MTR_2g099620 25.776 419 246 8 108 523 40 396 1.87e-39 149
MS.gene60930.t1 MTR_4g105100 27.578 446 264 10 86 526 175 566 2.17e-39 152
MS.gene60930.t1 MTR_4g125970 25.776 419 244 8 109 523 58 413 2.29e-39 149
MS.gene60930.t1 MTR_3g467550 26.484 438 247 11 103 525 111 488 7.95e-39 150
MS.gene60930.t1 MTR_2g007000 28.603 451 237 14 97 527 486 871 2.19e-38 151
MS.gene60930.t1 MTR_1g012420 25.647 425 257 6 107 529 45 412 3.14e-38 145
MS.gene60930.t1 MTR_2g032630 28.638 426 250 8 101 526 488 859 4.77e-38 150
MS.gene60930.t1 MTR_3g107420 25.882 425 256 6 107 529 45 412 6.23e-38 145
MS.gene60930.t1 MTR_2g041140 29.508 427 235 11 105 524 137 504 1.44e-37 148
MS.gene60930.t1 MTR_6g039840 29.147 422 252 10 109 526 110 488 2.05e-37 147
MS.gene60930.t1 MTR_1g054630 27.152 453 281 9 82 526 121 532 2.82e-37 146
MS.gene60930.t1 MTR_3g083690 27.803 446 265 9 102 535 138 538 3.60e-37 145
MS.gene60930.t1 MTR_3g467550 26.540 422 235 11 119 525 96 457 1.45e-36 143
MS.gene60930.t1 MTR_5g027840 29.730 407 227 12 108 509 103 455 1.65e-36 142
MS.gene60930.t1 MTR_7g057250 28.733 442 260 15 109 515 163 584 2.67e-36 144
MS.gene60930.t1 MTR_2g066050 26.908 498 280 12 67 551 5 431 3.77e-36 141
MS.gene60930.t1 MTR_7g076820 23.571 420 252 6 110 525 151 505 4.27e-36 141
MS.gene60930.t1 MTR_3g049990 28.873 426 238 12 105 524 148 514 8.43e-36 143
MS.gene60930.t1 MTR_7g076830 23.571 420 252 7 110 525 144 498 1.56e-35 140
MS.gene60930.t1 MTR_7g076800 23.571 420 252 7 110 525 142 496 1.88e-35 139
MS.gene60930.t1 MTR_4g010565 25.837 418 246 8 116 529 132 489 3.98e-35 138
MS.gene60930.t1 MTR_8g027775 26.526 426 258 9 110 529 103 479 4.23e-35 139
MS.gene60930.t1 MTR_4g131750 24.841 471 271 10 83 525 56 471 7.98e-35 137
MS.gene60930.t1 MTR_8g027770 27.635 427 252 12 110 529 202 578 1.27e-33 135
MS.gene60930.t1 MTR_8g097000 27.765 425 253 14 100 517 280 657 2.13e-33 135
MS.gene60930.t1 MTR_4g114120 29.340 409 243 11 101 504 132 499 1.52e-32 132
MS.gene60930.t1 MTR_2g055850 25.799 438 270 9 105 540 87 471 1.23e-31 129
MS.gene60930.t1 MTR_8g027780 27.189 434 262 11 102 529 98 483 1.73e-31 129
MS.gene60930.t1 MTR_1g102410 28.132 423 250 13 100 511 35 414 5.75e-31 127
MS.gene60930.t1 MTR_5g092560 28.960 404 228 11 132 531 99 447 1.15e-30 127
MS.gene60930.t1 MTR_7g100820 25.935 428 246 9 102 521 123 487 1.31e-30 126
MS.gene60930.t1 MTR_3g467550 25.945 397 219 11 103 484 111 447 1.10e-29 122
MS.gene60930.t1 MTR_6g056080 29.688 384 210 15 134 511 128 457 2.82e-29 123
MS.gene60930.t1 MTR_4g112340 25.659 417 252 10 127 539 115 477 3.09e-28 119
MS.gene60930.t1 MTR_3g071620 26.087 437 263 13 99 519 4 396 7.21e-28 118
MS.gene60930.t1 MTR_1g083130 25.503 447 245 9 108 515 25 422 1.98e-27 117
MS.gene60930.t1 MTR_1g061970 26.593 455 242 19 102 525 72 465 2.57e-27 115
MS.gene60930.t1 MTR_4g068990 28.141 398 221 11 132 522 99 438 9.48e-27 115
MS.gene60930.t1 MTR_4g105100 25.831 391 231 10 85 470 174 510 2.20e-26 113
MS.gene60930.t1 MTR_6g033070 25.290 431 247 13 98 516 131 498 9.00e-26 111
MS.gene60930.t1 MTR_2g043360 24.438 356 235 8 199 524 7 358 1.70e-24 107
MS.gene60930.t1 MTR_4g459350 28.947 228 144 7 102 322 34 250 4.56e-24 102
MS.gene60930.t1 MTR_4g049910 26.522 460 222 18 103 527 48 426 7.05e-24 105
MS.gene60930.t1 MTR_6g039840 27.976 336 202 9 195 526 13 312 3.64e-23 103
MS.gene60930.t1 MTR_8g018370 23.454 388 213 10 138 523 2 307 2.47e-22 98.6
MS.gene60930.t1 MTR_8g027780 25.441 397 245 10 102 492 98 449 5.32e-22 99.4
MS.gene60930.t1 MTR_3g118120 24.356 427 277 10 105 521 128 518 9.62e-21 96.3
MS.gene60930.t1 MTR_3g107420 30.769 208 113 4 116 322 55 232 1.09e-20 93.6
MS.gene60930.t1 MTR_0915s0010 22.874 341 194 7 125 461 5 280 1.14e-20 92.8
MS.gene60930.t1 MTR_4g077370 32.653 196 97 5 109 294 250 420 4.88e-19 90.1
MS.gene60930.t1 MTR_4g077370 29.798 198 85 6 109 294 2 157 2.33e-14 75.9
MS.gene60930.t1 MTR_8g032230 36.196 163 80 4 95 257 3 141 2.43e-18 83.2
MS.gene60930.t1 MTR_5g077690 25.616 406 246 12 147 521 71 451 5.23e-18 87.4
MS.gene60930.t1 MTR_1g046570 21.308 413 230 10 126 502 8 361 1.09e-17 85.9
MS.gene60930.t1 MTR_8g062720 30.052 193 117 6 109 293 91 273 8.84e-15 77.8
MS.gene60930.t1 MTR_4g112320 24.367 316 194 9 227 539 22 295 1.80e-13 72.8
MS.gene60930.t1 MTR_4g068990 26.953 256 143 6 270 522 13 227 5.01e-12 68.9
MS.gene60930.t1 MTR_0427s0010 35.417 96 47 2 445 525 104 199 7.11e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60930.t1 AT1G12770 60.323 557 208 8 1 550 1 551 0.0 654
MS.gene60930.t1 AT1G12770 60.323 557 208 8 1 550 1 551 0.0 654
MS.gene60930.t1 AT5G60990 28.851 409 242 7 108 515 9 369 1.03e-45 167
MS.gene60930.t1 AT3G19760 25.635 433 256 9 109 535 36 408 1.89e-45 165
MS.gene60930.t1 AT1G55150 28.271 428 254 8 99 526 90 464 8.06e-45 166
MS.gene60930.t1 AT1G55150 28.271 428 254 8 99 526 115 489 1.59e-44 165
MS.gene60930.t1 AT2G47330 27.327 505 292 12 29 515 136 583 3.48e-43 164
MS.gene60930.t1 AT4G09730 28.795 448 259 11 85 525 96 490 5.96e-43 162
MS.gene60930.t1 AT5G14610 28.230 418 246 6 109 526 170 533 7.29e-43 162
MS.gene60930.t1 AT5G14610 28.230 418 246 6 109 526 162 525 7.36e-43 162
MS.gene60930.t1 AT5G14610 28.230 418 246 6 109 526 162 525 8.35e-43 162
MS.gene60930.t1 AT1G31970 30.206 437 233 14 85 509 93 469 6.86e-42 158
MS.gene60930.t1 AT5G63120 29.439 428 249 10 99 526 156 530 8.75e-42 159
MS.gene60930.t1 AT5G51280 27.685 419 246 10 101 514 139 505 1.36e-41 158
MS.gene60930.t1 AT3G01540 28.708 418 244 8 109 526 159 522 3.24e-41 157
MS.gene60930.t1 AT3G01540 28.708 418 244 8 109 526 159 522 3.24e-41 157
MS.gene60930.t1 AT3G01540 28.708 418 244 8 109 526 159 522 3.24e-41 157
MS.gene60930.t1 AT3G13920 26.279 430 242 9 108 531 39 399 3.49e-41 154
MS.gene60930.t1 AT3G13920 26.279 430 242 9 108 531 43 403 3.52e-41 154
MS.gene60930.t1 AT3G01540 28.708 418 244 8 109 526 159 522 3.82e-41 157
MS.gene60930.t1 AT5G14610 28.019 414 241 7 113 526 38 394 7.92e-41 155
MS.gene60930.t1 AT5G14610 28.019 414 241 7 113 526 38 394 7.92e-41 155
MS.gene60930.t1 AT5G26742 27.824 478 271 12 59 534 68 473 1.14e-40 157
MS.gene60930.t1 AT5G26742 27.824 478 271 12 59 534 68 473 1.15e-40 157
MS.gene60930.t1 AT3G13920 26.128 421 241 9 108 521 29 386 1.71e-40 151
MS.gene60930.t1 AT3G13920 26.128 421 241 9 108 521 39 396 1.76e-40 152
MS.gene60930.t1 AT4G33370 27.294 425 240 11 101 514 90 456 2.63e-40 154
MS.gene60930.t1 AT1G54270 26.316 418 244 8 108 521 39 396 5.56e-40 150
MS.gene60930.t1 AT3G22330 27.292 480 279 13 50 525 54 467 7.15e-40 154
MS.gene60930.t1 AT3G06480 28.169 426 252 6 101 526 428 799 9.57e-40 155
MS.gene60930.t1 AT5G14610 28.250 400 233 6 127 526 248 593 1.10e-39 154
MS.gene60930.t1 AT2G33730 28.256 453 256 12 77 516 292 688 1.19e-39 154
MS.gene60930.t1 AT3G13920 26.128 421 236 10 108 521 39 391 2.90e-39 148
MS.gene60930.t1 AT2G40700 25.265 471 292 11 95 524 17 468 4.51e-39 151
MS.gene60930.t1 AT1G77030 27.294 425 244 9 110 529 30 394 6.73e-39 152
MS.gene60930.t1 AT2G45810 25.238 420 245 8 110 525 156 510 1.35e-38 149
MS.gene60930.t1 AT1G54270 26.316 418 239 9 108 521 39 391 1.51e-38 146
MS.gene60930.t1 AT1G72730 25.728 412 239 8 102 509 35 383 2.18e-38 146
MS.gene60930.t1 AT1G51380 25.485 412 240 9 108 515 22 370 3.93e-38 145
MS.gene60930.t1 AT3G61240 24.048 420 250 6 110 525 126 480 5.47e-38 146
MS.gene60930.t1 AT3G61240 24.048 420 250 6 110 525 126 480 5.47e-38 146
MS.gene60930.t1 AT1G20920 27.505 469 257 14 81 527 152 559 1.51e-37 148
MS.gene60930.t1 AT5G26742 28.713 404 228 10 133 534 35 380 5.50e-37 145
MS.gene60930.t1 AT3G22310 26.603 421 247 10 109 525 117 479 7.25e-37 145
MS.gene60930.t1 AT3G22310 26.603 421 247 10 109 525 117 479 8.27e-37 145
MS.gene60930.t1 AT5G11170 24.941 425 260 6 107 529 45 412 9.61e-37 142
MS.gene60930.t1 AT5G11200 24.941 425 260 6 107 529 45 412 9.61e-37 142
MS.gene60930.t1 AT1G16280 27.166 427 243 10 106 525 56 421 1.03e-36 143
MS.gene60930.t1 AT2G42520 27.465 426 270 7 101 526 152 538 1.44e-36 144
MS.gene60930.t1 AT2G42520 27.465 426 270 7 101 526 152 538 1.44e-36 144
MS.gene60930.t1 AT2G42520 27.465 426 270 7 101 526 152 538 1.44e-36 144
MS.gene60930.t1 AT1G28180 29.668 391 202 11 137 516 2 330 2.12e-36 139
MS.gene60930.t1 AT1G20920 27.556 450 243 13 97 527 512 897 2.93e-36 145
MS.gene60930.t1 AT1G20920 27.556 450 243 13 97 527 512 897 2.93e-36 145
MS.gene60930.t1 AT1G20920 27.556 450 243 13 97 527 512 897 2.93e-36 145
MS.gene60930.t1 AT1G20920 27.556 450 243 13 97 527 512 897 2.93e-36 145
MS.gene60930.t1 AT1G20920 27.556 450 243 13 97 527 512 897 2.93e-36 145
MS.gene60930.t1 AT1G20920 27.556 450 243 13 97 527 512 897 2.93e-36 145
MS.gene60930.t1 AT4G00660 23.571 420 252 7 110 525 133 487 3.18e-36 142
MS.gene60930.t1 AT4G00660 23.571 420 252 7 110 525 133 487 3.18e-36 142
MS.gene60930.t1 AT3G58570 27.146 431 265 9 101 526 139 525 1.37e-35 141
MS.gene60930.t1 AT5G11200 25.282 443 277 9 107 529 45 453 1.81e-35 139
MS.gene60930.t1 AT3G58510 27.230 426 267 8 102 526 145 528 2.14e-35 140
MS.gene60930.t1 AT3G58510 27.230 426 267 8 102 526 145 528 2.14e-35 140
MS.gene60930.t1 AT3G58510 27.230 426 267 8 102 526 145 528 2.14e-35 140
MS.gene60930.t1 AT1G63250 27.088 443 274 12 86 522 304 703 3.39e-35 141
MS.gene60930.t1 AT4G16630 28.375 437 238 13 100 525 159 531 4.67e-35 140
MS.gene60930.t1 AT5G63630 27.033 455 273 11 84 530 30 433 4.73e-35 139
MS.gene60930.t1 AT5G63630 27.033 455 273 11 84 530 224 627 2.01e-34 138
MS.gene60930.t1 AT2G07750 26.637 443 276 12 86 522 351 750 4.01e-34 138
MS.gene60930.t1 AT5G19210 25.835 449 253 10 102 525 70 463 6.07e-34 134
MS.gene60930.t1 AT5G11200 25.727 447 269 9 116 529 55 471 4.49e-33 132
MS.gene60930.t1 AT3G09720 26.636 428 243 10 102 521 131 495 8.07e-33 132
MS.gene60930.t1 AT3G09720 26.636 428 243 10 102 521 131 495 8.07e-33 132
MS.gene60930.t1 AT5G11170 25.455 385 228 6 147 529 2 329 2.06e-32 127
MS.gene60930.t1 AT5G14610 27.322 366 212 7 109 474 170 481 2.57e-32 130
MS.gene60930.t1 AT5G08610 27.536 414 249 10 110 517 384 752 1.67e-31 130
MS.gene60930.t1 AT3G18600 25.115 434 268 9 109 540 90 468 2.03e-31 128
MS.gene60930.t1 AT5G62190 28.704 432 240 14 96 519 88 459 6.88e-31 127
MS.gene60930.t1 AT5G63120 28.042 378 219 10 99 476 156 480 1.17e-30 125
MS.gene60930.t1 AT5G63120 28.042 378 219 10 99 476 156 480 1.17e-30 125
MS.gene60930.t1 AT5G63120 28.042 378 219 10 99 476 156 480 1.17e-30 125
MS.gene60930.t1 AT5G08620 28.095 420 239 13 110 517 82 450 2.65e-30 125
MS.gene60930.t1 AT1G71370 28.125 416 232 12 106 509 12 372 5.85e-30 124
MS.gene60930.t1 AT3G16840 26.218 431 284 10 109 515 191 611 1.48e-29 124
MS.gene60930.t1 AT3G16840 26.218 431 284 10 109 515 192 612 1.54e-29 124
MS.gene60930.t1 AT4G34910 26.207 435 231 9 106 499 44 429 1.82e-29 123
MS.gene60930.t1 AT5G65900 25.287 435 267 9 108 539 154 533 2.60e-29 122
MS.gene60930.t1 AT5G63120 27.957 372 215 10 99 470 156 474 3.30e-29 121
MS.gene60930.t1 AT5G05450 27.546 432 248 15 102 517 8 390 4.20e-29 122
MS.gene60930.t1 AT3G06480 26.649 379 224 6 101 479 428 752 4.85e-29 122
MS.gene60930.t1 AT3G02065 25.971 412 253 8 104 514 106 466 1.53e-28 119
MS.gene60930.t1 AT3G02065 25.971 412 253 8 104 514 106 466 1.53e-28 119
MS.gene60930.t1 AT3G53110 25.966 466 257 17 102 545 84 483 1.57e-27 116
MS.gene60930.t1 AT5G54910 27.506 429 245 13 108 529 71 440 4.25e-27 116
MS.gene60930.t1 AT3G02065 25.661 378 229 8 138 514 3 329 2.53e-22 99.4
MS.gene60930.t1 AT3G06980 24.408 422 267 11 93 502 359 740 3.24e-22 101
MS.gene60930.t1 AT4G15850 26.355 406 249 12 147 526 74 455 1.26e-20 95.9
MS.gene60930.t1 AT1G51380 24.528 318 173 9 108 421 22 276 5.94e-20 90.5
MS.gene60930.t1 AT3G09620 28.772 285 149 11 97 359 380 632 7.10e-20 94.4
MS.gene60930.t1 AT3G09620 41.250 80 47 0 448 527 657 736 2.99e-11 67.0
MS.gene60930.t1 AT3G09620 28.772 285 149 11 97 359 379 631 7.29e-20 94.4
MS.gene60930.t1 AT3G09620 41.250 80 47 0 448 527 656 735 3.04e-11 67.0
MS.gene60930.t1 AT1G71280 32.701 211 108 8 100 301 10 195 4.65e-19 89.0
MS.gene60930.t1 AT1G71280 32.701 211 108 8 100 301 10 195 1.93e-18 88.6
MS.gene60930.t1 AT1G51380 25.941 239 140 6 108 343 22 226 2.42e-18 86.3
MS.gene60930.t1 AT1G51380 25.941 239 140 6 108 343 22 226 3.07e-18 86.3
MS.gene60930.t1 AT1G59990 28.897 263 154 12 50 293 14 262 1.12e-11 67.8
MS.gene60930.t1 AT1G59990 28.897 263 154 12 50 293 14 262 1.24e-11 67.8

Find 148 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTATATTGTCTGTTAT+TGG 0.115915 2.4:-30891158 MS.gene60930:CDS
AGCTCCTTTCGTTCAATTTC+AGG 0.148174 2.4:-30890812 MS.gene60930:CDS
TACTCACGGCTGTCAATATT+TGG 0.203307 2.4:-30890853 MS.gene60930:CDS
CATGAAAACTATCACGAATT+TGG 0.207330 2.4:+30890431 None:intergenic
CACCATTCTTAAACTTCTTC+AGG 0.209602 2.4:+30890304 None:intergenic
ATCTCTTCACTTATAATCTT+TGG 0.215359 2.4:+30891479 None:intergenic
GATTATAAGTGAAGAGATTA+AGG 0.228510 2.4:-30891474 MS.gene60930:CDS
GGAAGATATGCACAGGTTAT+TGG 0.237733 2.4:-30890790 MS.gene60930:CDS
CACATCATGGTTCTTCAAAA+TGG 0.243161 2.4:+30891226 None:intergenic
TAGGGAGTTTGAGAAGATAT+TGG 0.257273 2.4:-30891021 MS.gene60930:CDS
AACCGAGCGTCAGTTAATTA+TGG 0.260490 2.4:-30890718 MS.gene60930:CDS
GCTGCCCTTACAACCGAAAA+TGG 0.262714 2.4:+30890681 None:intergenic
TTCCTATATTGTCTGTTATT+GGG 0.270162 2.4:-30891157 MS.gene60930:CDS
ATTCAGTCGTATACGGGTTC+AGG 0.307634 2.4:-30891200 MS.gene60930:CDS
GAATCATACTAAGCAGTTAA+AGG 0.308011 2.4:-30890409 MS.gene60930:CDS
GCAAAGTTTCTTGTACTTGT+TGG 0.311990 2.4:-30891629 MS.gene60930:CDS
GGTGGTGATGGTGGCGAGGC+AGG 0.314694 2.4:-30891107 MS.gene60930:CDS
TGGCGAGGCAGGGAAGAAAT+TGG 0.318352 2.4:-30891096 MS.gene60930:CDS
ATCTTCAAGAAGTGCTTCTC+AGG 0.328893 2.4:+30890004 None:intergenic
ATCTTCTTCTGAGGGTTTCA+AGG 0.339067 2.4:-30891435 MS.gene60930:CDS
TTAGTGAGAAGAAAAGTGTT+AGG 0.341371 2.4:-30891391 MS.gene60930:CDS
TGTGATAATTCAGTCGTATA+CGG 0.353834 2.4:-30891207 MS.gene60930:CDS
GAAGAAAAGTGTTAGGGTTA+AGG 0.355676 2.4:-30891384 MS.gene60930:CDS
AGCTTGGAATGCAGATAGTT+AGG 0.356904 2.4:-30891040 MS.gene60930:CDS
GCAGAGTTACATGGGGATCT+TGG 0.358850 2.4:-30890348 MS.gene60930:CDS
AGGGAGTTTGAGAAGATATT+GGG 0.360709 2.4:-30891020 MS.gene60930:CDS
AGATATTGGGAATGGAAAAC+AGG 0.370709 2.4:-30891007 MS.gene60930:CDS
CTTAAGGGCTTCTTCCTGCC+TGG 0.373888 2.4:+30890947 None:intergenic
TACCCCGTCTGTGCCATCTA+AGG 0.386698 2.4:-30890556 MS.gene60930:CDS
CTCTCTACCACAGCCTTAGA+TGG 0.388949 2.4:+30890543 None:intergenic
AACAAACCTGCTATTGTTGT+TGG 0.392785 2.4:-30890921 MS.gene60930:CDS
GGTGACAAATTGACTGGCTC+GGG 0.398398 2.4:+30890591 None:intergenic
TGAATTATCAGCAAGAGGTT+TGG 0.403922 2.4:-30890259 MS.gene60930:CDS
GGGCTTCTTCCTGCCTGGTT+CGG 0.413771 2.4:+30890952 None:intergenic
AAGGTTAAAAGAAAACCCTT+TGG 0.422516 2.4:-30891416 MS.gene60930:CDS
GAGATTAAGGTTAAGCCGTT+AGG 0.422743 2.4:-30891461 MS.gene60930:CDS
CAGAGTTACATGGGGATCTT+GGG 0.423541 2.4:-30890347 MS.gene60930:CDS
TGTTGAATTCTAGATTTGCT+TGG 0.428517 2.4:-30891580 MS.gene60930:CDS
ATTGAGAGGTTAGGAAAAGA+GGG 0.433157 2.4:-30891290 MS.gene60930:CDS
GATTGAGAGGTTAGGAAAAG+AGG 0.433951 2.4:-30891291 MS.gene60930:CDS
TTGTAAGGGCAGCTAAAAGC+TGG 0.437170 2.4:-30890671 MS.gene60930:CDS
AACCTCTCAATCAACACTTC+CGG 0.442396 2.4:+30891302 None:intergenic
AAAGTAGTTCAGCAGCTTGT+GGG 0.442423 2.4:-30890984 MS.gene60930:CDS
GAGCATGTCGGGAGGAGATC+GGG 0.449689 2.4:-30890768 MS.gene60930:CDS
ACCATTCTTAAACTTCTTCA+GGG 0.458193 2.4:+30890305 None:intergenic
TTTCAAAACCAACGCTTGAA+AGG 0.458359 2.4:+30891513 None:intergenic
GGCGAGGCAGGGAAGAAATT+GGG 0.458741 2.4:-30891095 MS.gene60930:CDS
TAGTGAGAAGAAAAGTGTTA+GGG 0.459906 2.4:-30891390 MS.gene60930:CDS
AACCTCTGACTCTTCACATA+TGG 0.461601 2.4:+30890125 None:intergenic
TTCTTCTCACTAACTCCAAA+GGG 0.462523 2.4:+30891401 None:intergenic
GCACACCGAGCTGGTCGAAC+CGG 0.465367 2.4:-30890177 MS.gene60930:CDS
GCTCCTTTCGTTCAATTTCA+GGG 0.466870 2.4:-30890811 MS.gene60930:CDS
GTTGCTCCTTCGAGGGAGCT+TGG 0.466886 2.4:-30891056 MS.gene60930:CDS
CATGGGGATCTTGGGAAGCT+TGG 0.468784 2.4:-30890339 MS.gene60930:CDS
GTGGTGATGGTGGCGAGGCA+GGG 0.468838 2.4:-30891106 MS.gene60930:CDS
GCTATTGTTGTTGGTACGCC+TGG 0.472411 2.4:-30890912 MS.gene60930:CDS
CAGTGATTGTTGCTCCTTCG+AGG 0.472921 2.4:-30891064 MS.gene60930:CDS
ACAGGTTATTGGAGCATGTC+GGG 0.474765 2.4:-30890779 MS.gene60930:CDS
GTGAAAGCTGCAGAGTTACA+TGG 0.483762 2.4:-30890357 MS.gene60930:CDS
CGGTGACAAATTGACTGGCT+CGG 0.485622 2.4:+30890590 None:intergenic
TTCAAAACCAACGCTTGAAA+GGG 0.486260 2.4:+30891514 None:intergenic
GAAAACGACTTAGCAGCAAA+AGG 0.488039 2.4:+30891338 None:intergenic
AAAACGACTTAGCAGCAAAA+GGG 0.490677 2.4:+30891339 None:intergenic
TGGGAGGTGCGAACCGAACC+AGG 0.490783 2.4:-30890965 MS.gene60930:CDS
TTCAACAAAGCCCTTCTCAT+AGG 0.495206 2.4:+30891596 None:intergenic
GTTTGAGAAGATATTGGGAA+TGG 0.495996 2.4:-30891015 MS.gene60930:CDS
GCTTGGAATGCAGATAGTTA+GGG 0.500523 2.4:-30891039 MS.gene60930:CDS
GAGAATCATTTCCTATGAGA+AGG 0.500582 2.4:-30891607 MS.gene60930:CDS
TCTTTGACCCTTTCAAGCGT+TGG 0.501352 2.4:-30891521 MS.gene60930:CDS
GTCTTCAAGTTGGAAGCCCG+TGG 0.502151 2.4:-30890381 MS.gene60930:CDS
TTCAGTCGTATACGGGTTCA+GGG 0.509478 2.4:-30891199 MS.gene60930:CDS
TTCTTAGCTTGAACAAGAAG+TGG 0.511531 2.4:+30890639 None:intergenic
ACAAAATTACAGCACGCAAT+CGG 0.514193 2.4:+30890066 None:intergenic
GAAAGTAGTTCAGCAGCTTG+TGG 0.518091 2.4:-30890985 MS.gene60930:CDS
GGGATCTTGGGAAGCTTGGT+AGG 0.520034 2.4:-30890335 MS.gene60930:CDS
TTAGGTTTATCTTCTTCTGA+GGG 0.521787 2.4:-30891443 MS.gene60930:CDS
ACCGGTCGGCTTGGTAGGAA+TGG 0.521840 2.4:-30890159 MS.gene60930:CDS
GCTGGTCGAACCGGTCGGCT+TGG 0.522034 2.4:-30890168 MS.gene60930:CDS
GGAGCATGTCGGGAGGAGAT+CGG 0.523954 2.4:-30890769 MS.gene60930:CDS
GTCTGTGCCATCTAAGGCTG+TGG 0.530947 2.4:-30890550 MS.gene60930:CDS
GTTAGGTTTATCTTCTTCTG+AGG 0.534553 2.4:-30891444 MS.gene60930:CDS
TGAGAGGTGAAAACCGTGAA+GGG 0.534640 2.4:-30891130 MS.gene60930:CDS
AGGTGCGAACCGAACCAGGC+AGG 0.536284 2.4:-30890961 MS.gene60930:CDS
AAAGGATGTTGTCTTCAAGT+TGG 0.536308 2.4:-30890391 MS.gene60930:CDS
TCTTCAAGTTGGAAGCCCGT+GGG 0.536477 2.4:-30890380 MS.gene60930:CDS
CTTCCCTGAAATTGAACGAA+AGG 0.537039 2.4:+30890808 None:intergenic
GTGATAATTCAGTCGTATAC+GGG 0.538275 2.4:-30891206 MS.gene60930:CDS
ACAAATGAATTATCAGCAAG+AGG 0.539693 2.4:-30890264 MS.gene60930:CDS
TTCCGGAAGTGTTGATTGAG+AGG 0.540200 2.4:-30891304 MS.gene60930:CDS
CTCTGCAGCTTTCACCCCAC+GGG 0.542325 2.4:+30890365 None:intergenic
GGCCCAATAACAGACAATAT+AGG 0.542850 2.4:+30891155 None:intergenic
TTTCTTCTCACTAACTCCAA+AGG 0.543267 2.4:+30891400 None:intergenic
TAGAATCTGTCGGTAAGTCC+AGG 0.543776 2.4:+30890208 None:intergenic
CAGCCTTAGATGGCACAGAC+GGG 0.544899 2.4:+30890553 None:intergenic
AACCATAATTAACTGACGCT+CGG 0.547188 2.4:+30890716 None:intergenic
CAGTAATAGTGTTTCAATGC+TGG 0.547631 2.4:+30890513 None:intergenic
GGCGTACCAACAACAATAGC+AGG 0.549925 2.4:+30890915 None:intergenic
GTCGAACCGGTCGGCTTGGT+AGG 0.553234 2.4:-30890164 MS.gene60930:CDS
ATTTCAGGGAAGATATGCAC+AGG 0.553303 2.4:-30890797 MS.gene60930:CDS
TGTAAGGGCAGCTAAAAGCT+GGG 0.556839 2.4:-30890670 MS.gene60930:CDS
CACAGGTTATTGGAGCATGT+CGG 0.558310 2.4:-30890780 MS.gene60930:CDS
CTAAGCTCAGCTATCCTACC+AGG 0.559177 2.4:+30890894 None:intergenic
AGTGATTGTTGCTCCTTCGA+GGG 0.560274 2.4:-30891063 MS.gene60930:CDS
ACTCTGCAGCTTTCACCCCA+CGG 0.561980 2.4:+30890364 None:intergenic
ATTCACTATGCACACCGAGC+TGG 0.565255 2.4:-30890186 MS.gene60930:CDS
AGAATCATTTCCTATGAGAA+GGG 0.566730 2.4:-30891606 MS.gene60930:CDS
TGAAAGCTGCAGAGTTACAT+GGG 0.567128 2.4:-30890356 MS.gene60930:CDS
ACCATTCCTACCAAGCCGAC+CGG 0.567408 2.4:+30890158 None:intergenic
ATGTGATCTTGTGGTAAACC+TGG 0.568642 2.4:-30890226 MS.gene60930:CDS
TTGTTGTTGGTACGCCTGGT+AGG 0.572319 2.4:-30890908 MS.gene60930:CDS
ACCCTGAAGAAGTTTAAGAA+TGG 0.575326 2.4:-30890306 MS.gene60930:CDS
GAAGTGTTGATTGAGAGGTT+AGG 0.576132 2.4:-30891299 MS.gene60930:CDS
GAGAAAGTGTATTCATGCAC+TGG 0.579567 2.4:-30890457 MS.gene60930:CDS
ATAGCTGAGCTTAGTGCAAG+TGG 0.580735 2.4:-30890885 MS.gene60930:CDS
GCCGACCGGTTCGACCAGCT+CGG 0.588019 2.4:+30890172 None:intergenic
ACGACTGAATTATCACATCA+TGG 0.589044 2.4:+30891213 None:intergenic
ACAGCCTTAGATGGCACAGA+CGG 0.591630 2.4:+30890552 None:intergenic
GAGCTCGGTGACAAATTGAC+TGG 0.595709 2.4:+30890585 None:intergenic
GTGACAAATTGACTGGCTCG+GGG 0.598998 2.4:+30890592 None:intergenic
CTGAGAGGTGAAAACCGTGA+AGG 0.603905 2.4:-30891131 MS.gene60930:CDS
TGCATTCCAAGCTCCCTCGA+AGG 0.605427 2.4:+30891050 None:intergenic
ATCAGCAAGAGGTTTGGATG+TGG 0.606020 2.4:-30890253 MS.gene60930:CDS
GACCATATGTGAAGAGTCAG+AGG 0.611581 2.4:-30890127 MS.gene60930:CDS
AGTGGGAAACTTCGTACTCA+CGG 0.617308 2.4:-30890867 MS.gene60930:CDS
AAACGACTTAGCAGCAAAAG+GGG 0.618984 2.4:+30891340 None:intergenic
TCTGTTATTGGGCCGCTGAG+AGG 0.624248 2.4:-30891146 MS.gene60930:CDS
GAAGAAGTTTAAGAATGGTG+AGG 0.624694 2.4:-30890301 MS.gene60930:CDS
GACTGATGTTCAGTCTGCTG+CGG 0.626483 2.4:-30891255 MS.gene60930:CDS
TCATGGTTCTTCAAAATGGT+AGG 0.626792 2.4:+30891230 None:intergenic
GAGAGGTGAAAACCGTGAAG+GGG 0.631669 2.4:-30891129 MS.gene60930:CDS
GCAGCAGACTGAACATCAGT+CGG 0.632121 2.4:+30891257 None:intergenic
TAATAGTGTTTCAATGCTGG+TGG 0.632317 2.4:+30890516 None:intergenic
GACGGGGTAGAACTCGAGCT+CGG 0.635162 2.4:+30890570 None:intergenic
ACCGAGCTGGTCGAACCGGT+CGG 0.635345 2.4:-30890173 MS.gene60930:CDS
TCTTCAAGAAGTGCTTCTCA+GGG 0.637636 2.4:+30890005 None:intergenic
GTAGTTCAGCAGCTTGTGGG+AGG 0.640465 2.4:-30890981 MS.gene60930:CDS
TAGCTGAGCTTAGTGCAAGT+GGG 0.645468 2.4:-30890884 MS.gene60930:CDS
AGAGGTGAAAACCGTGAAGG+GGG 0.652553 2.4:-30891128 MS.gene60930:CDS
CTTCAAGTTGGAAGCCCGTG+GGG 0.656339 2.4:-30890379 MS.gene60930:CDS
GCATAGTGAATAGAATCTGT+CGG 0.658249 2.4:+30890198 None:intergenic
TCAGAAGAAGATAAACCTAA+CGG 0.660328 2.4:+30891446 None:intergenic
GTAAGGGCAGCTAAAAGCTG+GGG 0.677223 2.4:-30890669 MS.gene60930:CDS
GGTTATTGGAGCATGTCGGG+AGG 0.679100 2.4:-30890776 MS.gene60930:CDS
AGCCTTAGATGGCACAGACG+GGG 0.682132 2.4:+30890554 None:intergenic
TGTGGCAGAATGTGATCTTG+TGG 0.686568 2.4:-30890235 MS.gene60930:CDS
AACACTATTACTGTGTAGTG+AGG 0.701078 2.4:-30890503 MS.gene60930:CDS
GAAAGCTGCAGAGTTACATG+GGG 0.706167 2.4:-30890355 MS.gene60930:CDS
TGACAAATTGACTGGCTCGG+GGG 0.719094 2.4:+30890593 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAGCATTTGAGTTA+TGG + chr2.4:30890919-30890938 None:intergenic 20.0%
! ATCTCTTCACTTATAATCTT+TGG + chr2.4:30890179-30890198 None:intergenic 25.0%
! GATTATAAGTGAAGAGATTA+AGG - chr2.4:30890181-30890200 MS.gene60930:CDS 25.0%
! TTCCTATATTGTCTGTTATT+GGG - chr2.4:30890498-30890517 MS.gene60930:CDS 25.0%
AAGGTTAAAAGAAAACCCTT+TGG - chr2.4:30890239-30890258 MS.gene60930:CDS 30.0%
ACAAATGAATTATCAGCAAG+AGG - chr2.4:30891391-30891410 MS.gene60930:CDS 30.0%
ACCATTCTTAAACTTCTTCA+GGG + chr2.4:30891353-30891372 None:intergenic 30.0%
AGAATCATTTCCTATGAGAA+GGG - chr2.4:30890049-30890068 MS.gene60930:CDS 30.0%
CATGAAAACTATCACGAATT+TGG + chr2.4:30891227-30891246 None:intergenic 30.0%
CTTCCTATATTGTCTGTTAT+TGG - chr2.4:30890497-30890516 MS.gene60930:CDS 30.0%
GAATCATACTAAGCAGTTAA+AGG - chr2.4:30891246-30891265 MS.gene60930:CDS 30.0%
TCAGAAGAAGATAAACCTAA+CGG + chr2.4:30890212-30890231 None:intergenic 30.0%
TGTGATAATTCAGTCGTATA+CGG - chr2.4:30890448-30890467 MS.gene60930:CDS 30.0%
TTAGGTTTATCTTCTTCTGA+GGG - chr2.4:30890212-30890231 MS.gene60930:CDS 30.0%
! TGTTGAATTCTAGATTTGCT+TGG - chr2.4:30890075-30890094 MS.gene60930:CDS 30.0%
!! AGCAGGTTTGTTTTTCTTAA+GGG + chr2.4:30890726-30890745 None:intergenic 30.0%
!! TAGCAGGTTTGTTTTTCTTA+AGG + chr2.4:30890727-30890746 None:intergenic 30.0%
!! TAGTGAGAAGAAAAGTGTTA+GGG - chr2.4:30890265-30890284 MS.gene60930:CDS 30.0%
!! TTAGTGAGAAGAAAAGTGTT+AGG - chr2.4:30890264-30890283 MS.gene60930:CDS 30.0%
AAAACGACTTAGCAGCAAAA+GGG + chr2.4:30890319-30890338 None:intergenic 35.0%
AAAGGATGTTGTCTTCAAGT+TGG - chr2.4:30891264-30891283 MS.gene60930:CDS 35.0%
AACAAACCTGCTATTGTTGT+TGG - chr2.4:30890734-30890753 MS.gene60930:CDS 35.0%
AACACTATTACTGTGTAGTG+AGG - chr2.4:30891152-30891171 MS.gene60930:CDS 35.0%
AACCATAATTAACTGACGCT+CGG + chr2.4:30890942-30890961 None:intergenic 35.0%
ACAAAATTACAGCACGCAAT+CGG + chr2.4:30891592-30891611 None:intergenic 35.0%
ACCCTGAAGAAGTTTAAGAA+TGG - chr2.4:30891349-30891368 MS.gene60930:CDS 35.0%
ACGACTGAATTATCACATCA+TGG + chr2.4:30890445-30890464 None:intergenic 35.0%
AGATATTGGGAATGGAAAAC+AGG - chr2.4:30890648-30890667 MS.gene60930:CDS 35.0%
AGGGAGTTTGAGAAGATATT+GGG - chr2.4:30890635-30890654 MS.gene60930:CDS 35.0%
ATTGAGAGGTTAGGAAAAGA+GGG - chr2.4:30890365-30890384 MS.gene60930:CDS 35.0%
CACATCATGGTTCTTCAAAA+TGG + chr2.4:30890432-30890451 None:intergenic 35.0%
CACCATTCTTAAACTTCTTC+AGG + chr2.4:30891354-30891373 None:intergenic 35.0%
CAGTAATAGTGTTTCAATGC+TGG + chr2.4:30891145-30891164 None:intergenic 35.0%
GAAGAAGTTTAAGAATGGTG+AGG - chr2.4:30891354-30891373 MS.gene60930:CDS 35.0%
GAGAATCATTTCCTATGAGA+AGG - chr2.4:30890048-30890067 MS.gene60930:CDS 35.0%
GCAAAGTTTCTTGTACTTGT+TGG - chr2.4:30890026-30890045 MS.gene60930:CDS 35.0%
GCATAGTGAATAGAATCTGT+CGG + chr2.4:30891460-30891479 None:intergenic 35.0%
GTGATAATTCAGTCGTATAC+GGG - chr2.4:30890449-30890468 MS.gene60930:CDS 35.0%
GTTAGGTTTATCTTCTTCTG+AGG - chr2.4:30890211-30890230 MS.gene60930:CDS 35.0%
GTTTGAGAAGATATTGGGAA+TGG - chr2.4:30890640-30890659 MS.gene60930:CDS 35.0%
TAATAGTGTTTCAATGCTGG+TGG + chr2.4:30891142-30891161 None:intergenic 35.0%
TAGGGAGTTTGAGAAGATAT+TGG - chr2.4:30890634-30890653 MS.gene60930:CDS 35.0%
TCATGGTTCTTCAAAATGGT+AGG + chr2.4:30890428-30890447 None:intergenic 35.0%
TGAATTATCAGCAAGAGGTT+TGG - chr2.4:30891396-30891415 MS.gene60930:CDS 35.0%
TTCAAAACCAACGCTTGAAA+GGG + chr2.4:30890144-30890163 None:intergenic 35.0%
TTCTTCTCACTAACTCCAAA+GGG + chr2.4:30890257-30890276 None:intergenic 35.0%
TTTCAAAACCAACGCTTGAA+AGG + chr2.4:30890145-30890164 None:intergenic 35.0%
TTTCTTCTCACTAACTCCAA+AGG + chr2.4:30890258-30890277 None:intergenic 35.0%
! TGTACCATTTTCGGTTGTAA+GGG - chr2.4:30890970-30890989 MS.gene60930:CDS 35.0%
! TTCTGCAACTGTACCATTTT+CGG - chr2.4:30890961-30890980 MS.gene60930:CDS 35.0%
! TTCTTAGCTTGAACAAGAAG+TGG + chr2.4:30891019-30891038 None:intergenic 35.0%
!! GAAGAAAAGTGTTAGGGTTA+AGG - chr2.4:30890271-30890290 MS.gene60930:CDS 35.0%
!! TTTTGCTGCTAAGTCGTTTT+CGG - chr2.4:30890319-30890338 MS.gene60930:CDS 35.0%
AAACGACTTAGCAGCAAAAG+GGG + chr2.4:30890318-30890337 None:intergenic 40.0%
AAAGTAGTTCAGCAGCTTGT+GGG - chr2.4:30890671-30890690 MS.gene60930:CDS 40.0%
AACCGAGCGTCAGTTAATTA+TGG - chr2.4:30890937-30890956 MS.gene60930:CDS 40.0%
AACCTCTCAATCAACACTTC+CGG + chr2.4:30890356-30890375 None:intergenic 40.0%
AACCTCTGACTCTTCACATA+TGG + chr2.4:30891533-30891552 None:intergenic 40.0%
AGCTCCTTTCGTTCAATTTC+AGG - chr2.4:30890843-30890862 MS.gene60930:CDS 40.0%
AGCTTGGAATGCAGATAGTT+AGG - chr2.4:30890615-30890634 MS.gene60930:CDS 40.0%
ATGTGATCTTGTGGTAAACC+TGG - chr2.4:30891429-30891448 MS.gene60930:CDS 40.0%
ATTTCAGGGAAGATATGCAC+AGG - chr2.4:30890858-30890877 MS.gene60930:CDS 40.0%
CTTCCCTGAAATTGAACGAA+AGG + chr2.4:30890850-30890869 None:intergenic 40.0%
GAAAACGACTTAGCAGCAAA+AGG + chr2.4:30890320-30890339 None:intergenic 40.0%
GAGATTAAGGTTAAGCCGTT+AGG - chr2.4:30890194-30890213 MS.gene60930:CDS 40.0%
GATTGAGAGGTTAGGAAAAG+AGG - chr2.4:30890364-30890383 MS.gene60930:CDS 40.0%
GCTCCTTTCGTTCAATTTCA+GGG - chr2.4:30890844-30890863 MS.gene60930:CDS 40.0%
GCTTGGAATGCAGATAGTTA+GGG - chr2.4:30890616-30890635 MS.gene60930:CDS 40.0%
GGAAGATATGCACAGGTTAT+TGG - chr2.4:30890865-30890884 MS.gene60930:CDS 40.0%
GGCCCAATAACAGACAATAT+AGG + chr2.4:30890503-30890522 None:intergenic 40.0%
TACTCACGGCTGTCAATATT+TGG - chr2.4:30890802-30890821 MS.gene60930:CDS 40.0%
TGAAAGCTGCAGAGTTACAT+GGG - chr2.4:30891299-30891318 MS.gene60930:CDS 40.0%
TTCAACAAAGCCCTTCTCAT+AGG + chr2.4:30890062-30890081 None:intergenic 40.0%
! ATCTTCTTCTGAGGGTTTCA+AGG - chr2.4:30890220-30890239 MS.gene60930:CDS 40.0%
! CTGTACCATTTTCGGTTGTA+AGG - chr2.4:30890969-30890988 MS.gene60930:CDS 40.0%
! GAGAAAGTGTATTCATGCAC+TGG - chr2.4:30891198-30891217 MS.gene60930:CDS 40.0%
! GCGTGCTGTAATTTTGTTGA+GGG - chr2.4:30891595-30891614 MS.gene60930:CDS 40.0%
! GTTTTCGGAACTTGGTATTC+CGG - chr2.4:30890334-30890353 MS.gene60930:CDS 40.0%
! TGCGTGCTGTAATTTTGTTG+AGG - chr2.4:30891594-30891613 MS.gene60930:CDS 40.0%
!! GAAGTGTTGATTGAGAGGTT+AGG - chr2.4:30890356-30890375 MS.gene60930:CDS 40.0%
ACAGGTTATTGGAGCATGTC+GGG - chr2.4:30890876-30890895 MS.gene60930:CDS 45.0%
AGTGATTGTTGCTCCTTCGA+GGG - chr2.4:30890592-30890611 MS.gene60930:CDS 45.0%
AGTGGGAAACTTCGTACTCA+CGG - chr2.4:30890788-30890807 MS.gene60930:CDS 45.0%
ATAGCTGAGCTTAGTGCAAG+TGG - chr2.4:30890770-30890789 MS.gene60930:CDS 45.0%
ATCAGCAAGAGGTTTGGATG+TGG - chr2.4:30891402-30891421 MS.gene60930:CDS 45.0%
ATTCAGTCGTATACGGGTTC+AGG - chr2.4:30890455-30890474 MS.gene60930:CDS 45.0%
CACAGGTTATTGGAGCATGT+CGG - chr2.4:30890875-30890894 MS.gene60930:CDS 45.0%
CAGAGTTACATGGGGATCTT+GGG - chr2.4:30891308-30891327 MS.gene60930:CDS 45.0%
GAAAGCTGCAGAGTTACATG+GGG - chr2.4:30891300-30891319 MS.gene60930:CDS 45.0%
GAAAGTAGTTCAGCAGCTTG+TGG - chr2.4:30890670-30890689 MS.gene60930:CDS 45.0%
GACCATATGTGAAGAGTCAG+AGG - chr2.4:30891528-30891547 MS.gene60930:CDS 45.0%
GTGAAAGCTGCAGAGTTACA+TGG - chr2.4:30891298-30891317 MS.gene60930:CDS 45.0%
TAGAATCTGTCGGTAAGTCC+AGG + chr2.4:30891450-30891469 None:intergenic 45.0%
TAGCTGAGCTTAGTGCAAGT+GGG - chr2.4:30890771-30890790 MS.gene60930:CDS 45.0%
TCTTTGACCCTTTCAAGCGT+TGG - chr2.4:30890134-30890153 MS.gene60930:CDS 45.0%
TGAGAGGTGAAAACCGTGAA+GGG - chr2.4:30890525-30890544 MS.gene60930:CDS 45.0%
TGTAAGGGCAGCTAAAAGCT+GGG - chr2.4:30890985-30891004 MS.gene60930:CDS 45.0%
TGTGGCAGAATGTGATCTTG+TGG - chr2.4:30891420-30891439 MS.gene60930:CDS 45.0%
TTCAGTCGTATACGGGTTCA+GGG - chr2.4:30890456-30890475 MS.gene60930:CDS 45.0%
TTGTAAGGGCAGCTAAAAGC+TGG - chr2.4:30890984-30891003 MS.gene60930:CDS 45.0%
!! GCTAAGTCGTTTTCGGAACT+TGG - chr2.4:30890326-30890345 MS.gene60930:CDS 45.0%
!! TTCCGGAAGTGTTGATTGAG+AGG - chr2.4:30890351-30890370 MS.gene60930:CDS 45.0%
ACAGCCTTAGATGGCACAGA+CGG + chr2.4:30891106-30891125 None:intergenic 50.0%
AGAGGTGAAAACCGTGAAGG+GGG - chr2.4:30890527-30890546 MS.gene60930:CDS 50.0%
ATTCACTATGCACACCGAGC+TGG - chr2.4:30891469-30891488 MS.gene60930:CDS 50.0%
CAGTGATTGTTGCTCCTTCG+AGG - chr2.4:30890591-30890610 MS.gene60930:CDS 50.0%
CTAAGCTCAGCTATCCTACC+AGG + chr2.4:30890764-30890783 None:intergenic 50.0%
CTCTCTACCACAGCCTTAGA+TGG + chr2.4:30891115-30891134 None:intergenic 50.0%
CTGAGAGGTGAAAACCGTGA+AGG - chr2.4:30890524-30890543 MS.gene60930:CDS 50.0%
GACTGATGTTCAGTCTGCTG+CGG - chr2.4:30890400-30890419 MS.gene60930:CDS 50.0%
GAGAGGTGAAAACCGTGAAG+GGG - chr2.4:30890526-30890545 MS.gene60930:CDS 50.0%
GCAGAGTTACATGGGGATCT+TGG - chr2.4:30891307-30891326 MS.gene60930:CDS 50.0%
GCAGCAGACTGAACATCAGT+CGG + chr2.4:30890401-30890420 None:intergenic 50.0%
GCTGCCCTTACAACCGAAAA+TGG + chr2.4:30890977-30890996 None:intergenic 50.0%
GGCGTACCAACAACAATAGC+AGG + chr2.4:30890743-30890762 None:intergenic 50.0%
GTAAGGGCAGCTAAAAGCTG+GGG - chr2.4:30890986-30891005 MS.gene60930:CDS 50.0%
TCGGGGGACAAAGTTTCAAG+TGG + chr2.4:30891049-30891068 None:intergenic 50.0%
! CGGTGACAAATTGACTGGCT+CGG + chr2.4:30891068-30891087 None:intergenic 50.0%
! GAGCTCGGTGACAAATTGAC+TGG + chr2.4:30891073-30891092 None:intergenic 50.0%
! GCTATTGTTGTTGGTACGCC+TGG - chr2.4:30890743-30890762 MS.gene60930:CDS 50.0%
! GGTGACAAATTGACTGGCTC+GGG + chr2.4:30891067-30891086 None:intergenic 50.0%
! TCACGGTTTTCACCTCTCAG+CGG + chr2.4:30890524-30890543 None:intergenic 50.0%
! TTGTTGTTGGTACGCCTGGT+AGG - chr2.4:30890747-30890766 MS.gene60930:CDS 50.0%
!! GTGACAAATTGACTGGCTCG+GGG + chr2.4:30891066-30891085 None:intergenic 50.0%
!! TCTTCAAGTTGGAAGCCCGT+GGG - chr2.4:30891275-30891294 MS.gene60930:CDS 50.0%
!! TGACAAATTGACTGGCTCGG+GGG + chr2.4:30891065-30891084 None:intergenic 50.0%
ACCATTCCTACCAAGCCGAC+CGG + chr2.4:30891500-30891519 None:intergenic 55.0%
ACTCTGCAGCTTTCACCCCA+CGG + chr2.4:30891294-30891313 None:intergenic 55.0%
AGCCTTAGATGGCACAGACG+GGG + chr2.4:30891104-30891123 None:intergenic 55.0%
CAGCCTTAGATGGCACAGAC+GGG + chr2.4:30891105-30891124 None:intergenic 55.0%
CATGGGGATCTTGGGAAGCT+TGG - chr2.4:30891316-30891335 MS.gene60930:CDS 55.0%
CTTAAGGGCTTCTTCCTGCC+TGG + chr2.4:30890711-30890730 None:intergenic 55.0%
GGCGAGGCAGGGAAGAAATT+GGG - chr2.4:30890560-30890579 MS.gene60930:CDS 55.0%
GGGATCTTGGGAAGCTTGGT+AGG - chr2.4:30891320-30891339 MS.gene60930:CDS 55.0%
GGTTATTGGAGCATGTCGGG+AGG - chr2.4:30890879-30890898 MS.gene60930:CDS 55.0%
GTAGTTCAGCAGCTTGTGGG+AGG - chr2.4:30890674-30890693 MS.gene60930:CDS 55.0%
TACCCCGTCTGTGCCATCTA+AGG - chr2.4:30891099-30891118 MS.gene60930:CDS 55.0%
TCTGTTATTGGGCCGCTGAG+AGG - chr2.4:30890509-30890528 MS.gene60930:CDS 55.0%
TGCATTCCAAGCTCCCTCGA+AGG + chr2.4:30890608-30890627 None:intergenic 55.0%
TGGCGAGGCAGGGAAGAAAT+TGG - chr2.4:30890559-30890578 MS.gene60930:CDS 55.0%
! GTCTGTGCCATCTAAGGCTG+TGG - chr2.4:30891105-30891124 MS.gene60930:CDS 55.0%
!! CTTCAAGTTGGAAGCCCGTG+GGG - chr2.4:30891276-30891295 MS.gene60930:CDS 55.0%
!! GTCTTCAAGTTGGAAGCCCG+TGG - chr2.4:30891274-30891293 MS.gene60930:CDS 55.0%
AACCGTGAAGGGGGTGGTGA+TGG - chr2.4:30890536-30890555 MS.gene60930:CDS 60.0%
CACCATCACCACCCCCTTCA+CGG + chr2.4:30890541-30890560 None:intergenic 60.0%
CTCTGCAGCTTTCACCCCAC+GGG + chr2.4:30891293-30891312 None:intergenic 60.0%
GACGGGGTAGAACTCGAGCT+CGG + chr2.4:30891088-30891107 None:intergenic 60.0%
GAGCATGTCGGGAGGAGATC+GGG - chr2.4:30890887-30890906 MS.gene60930:CDS 60.0%
GGAGCATGTCGGGAGGAGAT+CGG - chr2.4:30890886-30890905 MS.gene60930:CDS 60.0%
GGGCTTCTTCCTGCCTGGTT+CGG + chr2.4:30890706-30890725 None:intergenic 60.0%
GGTGAAAACCGTGAAGGGGG+TGG - chr2.4:30890530-30890549 MS.gene60930:CDS 60.0%
GTTGCTCCTTCGAGGGAGCT+TGG - chr2.4:30890599-30890618 MS.gene60930:CDS 60.0%
!! ACCGGTCGGCTTGGTAGGAA+TGG - chr2.4:30891496-30891515 MS.gene60930:CDS 60.0%
ACCGAGCTGGTCGAACCGGT+CGG - chr2.4:30891482-30891501 MS.gene60930:CDS 65.0%
AGGTGCGAACCGAACCAGGC+AGG - chr2.4:30890694-30890713 MS.gene60930:CDS 65.0%
GCACACCGAGCTGGTCGAAC+CGG - chr2.4:30891478-30891497 MS.gene60930:CDS 65.0%
! TGGGAGGTGCGAACCGAACC+AGG - chr2.4:30890690-30890709 MS.gene60930:CDS 65.0%
!! CGTGAAGGGGGTGGTGATGG+TGG - chr2.4:30890539-30890558 MS.gene60930:CDS 65.0%
!! GTCGAACCGGTCGGCTTGGT+AGG - chr2.4:30891491-30891510 MS.gene60930:CDS 65.0%
!! GTGGTGATGGTGGCGAGGCA+GGG - chr2.4:30890549-30890568 MS.gene60930:CDS 65.0%
GCTGGTCGAACCGGTCGGCT+TGG - chr2.4:30891487-30891506 MS.gene60930:CDS 70.0%
!! AGGGGGTGGTGATGGTGGCG+AGG - chr2.4:30890544-30890563 MS.gene60930:CDS 70.0%
!! GCCGACCGGTTCGACCAGCT+CGG + chr2.4:30891486-30891505 None:intergenic 70.0%
!! GGTGGTGATGGTGGCGAGGC+AGG - chr2.4:30890548-30890567 MS.gene60930:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 30890008 30891669 30890008 ID=MS.gene60930
chr2.4 mRNA 30890008 30891669 30890008 ID=MS.gene60930.t1;Parent=MS.gene60930
chr2.4 exon 30890008 30891669 30890008 ID=MS.gene60930.t1.exon1;Parent=MS.gene60930.t1
chr2.4 CDS 30890008 30891669 30890008 ID=cds.MS.gene60930.t1;Parent=MS.gene60930.t1
Gene Sequence

>MS.gene60930

ATGCAATCTTTGGTTTCAGCAAAGTTTCTTGTACTTGTTGGAGAATCATTTCCTATGAGAAGGGCTTTGTTGAATTCTAGATTTGCTTGGTTTCATAGTAGTGTGAAATGTTTGAACAAACATGATTCTTTGACCCTTTCAAGCGTTGGTTTTGAAAGTGAAATTGAGCCAAAGATTATAAGTGAAGAGATTAAGGTTAAGCCGTTAGGTTTATCTTCTTCTGAGGGTTTCAAGGTTAAAAGAAAACCCTTTGGAGTTAGTGAGAAGAAAAGTGTTAGGGTTAAGGAGAGAAAAGAAATAGAGAATGCCCCTTTTGCTGCTAAGTCGTTTTCGGAACTTGGTATTCCGGAAGTGTTGATTGAGAGGTTAGGAAAAGAGGGTTTTAATGTTCCGACTGATGTTCAGTCTGCTGCGGTGCCTACCATTTTGAAGAACCATGATGTGATAATTCAGTCGTATACGGGTTCAGGGAAGACATTAGCTTATCTGCTTCCTATATTGTCTGTTATTGGGCCGCTGAGAGGTGAAAACCGTGAAGGGGGTGGTGATGGTGGCGAGGCAGGGAAGAAATTGGGAGTAGAAGCAGTGATTGTTGCTCCTTCGAGGGAGCTTGGAATGCAGATAGTTAGGGAGTTTGAGAAGATATTGGGAATGGAAAACAGGAAAGTAGTTCAGCAGCTTGTGGGAGGTGCGAACCGAACCAGGCAGGAAGAAGCCCTTAAGAAAAACAAACCTGCTATTGTTGTTGGTACGCCTGGTAGGATAGCTGAGCTTAGTGCAAGTGGGAAACTTCGTACTCACGGCTGTCAATATTTGGTATTAGATGAAGTTGACGAGCTCCTTTCGTTCAATTTCAGGGAAGATATGCACAGGTTATTGGAGCATGTCGGGAGGAGATCGGGTGCAGACCATAACTCAAATGCTAAAAAAACCGAGCGTCAGTTAATTATGGTTTCTGCAACTGTACCATTTTCGGTTGTAAGGGCAGCTAAAAGCTGGGGTTCTGAACCACTTCTTGTTCAAGCTAAGAAAATTGTACCACTTGAAACTTTGTCCCCCGAGCCAGTCAATTTGTCACCGAGCTCGAGTTCTACCCCGTCTGTGCCATCTAAGGCTGTGGTAGAGAGTCTACCACCAGCATTGAAACACTATTACTGTGTAGTGAGGTTGCAGCACAAAGTTGATACATTGAGAAAGTGTATTCATGCACTGGATGCCAAATTCGTGATAGTTTTCATGAATCATACTAAGCAGTTAAAGGATGTTGTCTTCAAGTTGGAAGCCCGTGGGGTGAAAGCTGCAGAGTTACATGGGGATCTTGGGAAGCTTGGTAGGTCGACAACCCTGAAGAAGTTTAAGAATGGTGAGGTTAGAGTTCTTGTGACAAATGAATTATCAGCAAGAGGTTTGGATGTGGCAGAATGTGATCTTGTGGTAAACCTGGACTTACCGACAGATTCTATTCACTATGCACACCGAGCTGGTCGAACCGGTCGGCTTGGTAGGAATGGTACTGTGCTGACCATATGTGAAGAGTCAGAGGTTTTTGTTGTGAGAAAATTGAAGAAGCAACTTGAAATACCGATTGCGTGCTGTAATTTTGTTGAGGGAAAGCTTACTATTACTGAAGAAGAAGAGAATGCCCTGAGAAGCACTTCTTGA
Protein sequence

>MS.gene60930.t1

MQSLVSAKFLVLVGESFPMRRALLNSRFAWFHSSVKCLNKHDSLTLSSVGFESEIEPKIISEEIKVKPLGLSSSEGFKVKRKPFGVSEKKSVRVKERKEIENAPFAAKSFSELGIPEVLIERLGKEGFNVPTDVQSAAVPTILKNHDVIIQSYTGSGKTLAYLLPILSVIGPLRGENREGGGDGGEAGKKLGVEAVIVAPSRELGMQIVREFEKILGMENRKVVQQLVGGANRTRQEEALKKNKPAIVVGTPGRIAELSASGKLRTHGCQYLVLDEVDELLSFNFREDMHRLLEHVGRRSGADHNSNAKKTERQLIMVSATVPFSVVRAAKSWGSEPLLVQAKKIVPLETLSPEPVNLSPSSSSTPSVPSKAVVESLPPALKHYYCVVRLQHKVDTLRKCIHALDAKFVIVFMNHTKQLKDVVFKLEARGVKAAELHGDLGKLGRSTTLKKFKNGEVRVLVTNELSARGLDVAECDLVVNLDLPTDSIHYAHRAGRTGRLGRNGTVLTICEESEVFVVRKLKKQLEIPIACCNFVEGKLTITEEEENALRSTS