AlfalfaGEDB | MS.gene61107

M. sativa cultivar XinJiangDaYe / MS.gene61107

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61107.t1	RHN60978.1	94.6	149	4	2	1	148	1	146	1.50E-65	258.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61107.t1	Q2HU65	93.4	152	5	4	1	148	1	151	6.8e-67	254.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61107.t1	A0A396I838	94.6	149	4	2	1	148	1	146	1.1e-65	258.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053348	MS.gene61107	0.81192	5.50E-51	-1.69E-46
MS.gene054551	MS.gene61107	0.802477	5.51E-49	-1.69E-46
MS.gene054593	MS.gene61107	0.808595	2.87E-50	-1.69E-46
MS.gene054633	MS.gene61107	0.802693	4.97E-49	-1.69E-46
MS.gene055578	MS.gene61107	0.831989	1.22E-55	-1.69E-46
MS.gene055957	MS.gene61107	0.83089	2.28E-55	-1.69E-46
MS.gene056041	MS.gene61107	0.839778	1.29E-57	-1.69E-46
MS.gene05640	MS.gene61107	0.835245	1.88E-56	-1.69E-46
MS.gene057458	MS.gene61107	0.815083	1.11E-51	-1.69E-46
MS.gene058013	MS.gene61107	0.818631	1.77E-52	-1.69E-46
MS.gene058128	MS.gene61107	0.805045	1.61E-49	-1.69E-46
MS.gene059553	MS.gene61107	0.808091	3.67E-50	-1.69E-46
MS.gene060473	MS.gene61107	0.82271	2.05E-53	-1.69E-46
MS.gene060565	MS.gene61107	0.809926	1.49E-50	-1.69E-46
MS.gene06093	MS.gene61107	0.803726	3.04E-49	-1.69E-46
MS.gene061099	MS.gene61107	0.802929	4.44E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61107.t1	MTR_4g063280	96.454	141	4	1	9	148	3	143	1.93e-91	262
MS.gene61107.t1	MTR_2g096570	90.066	151	12	2	1	148	1	151	1.39e-81	237
MS.gene61107.t1	MTR_2g096610	88.514	148	15	2	1	148	1	146	1.99e-81	236
MS.gene61107.t1	MTR_4g071150	90.728	151	10	3	1	148	1	150	1.26e-79	232
MS.gene61107.t1	MTR_4g064005	90.604	149	10	2	1	148	1	146	3.22e-79	231
MS.gene61107.t1	MTR_4g063410	89.933	149	11	2	1	148	1	146	7.02e-79	230
MS.gene61107.t1	MTR_4g064967	90.604	149	10	2	1	148	1	146	1.69e-78	229
MS.gene61107.t1	MTR_8g086640	93.939	132	7	1	1	131	1	132	5.39e-74	218
MS.gene61107.t1	MTR_7g113160	82.707	133	22	1	10	142	13	144	8.44e-74	217
MS.gene61107.t1	MTR_7g108320	74.219	128	30	1	4	131	6	130	9.20e-61	184
MS.gene61107.t1	MTR_7g114060	74.419	129	30	1	3	131	2	127	1.45e-60	183
MS.gene61107.t1	MTR_7g114040	74.419	129	30	1	3	131	2	127	1.45e-60	183
MS.gene61107.t1	MTR_7g093910	73.810	126	31	1	4	129	5	128	1.23e-59	181
MS.gene61107.t1	MTR_2g082510	72.951	122	30	1	18	136	11	132	5.68e-58	177
MS.gene61107.t1	MTR_2g082220	78.704	108	23	0	21	128	14	121	2.21e-57	175
MS.gene61107.t1	MTR_6g005150	66.393	122	31	3	23	142	26	139	2.69e-48	152
MS.gene61107.t1	MTR_4g063300	80.000	105	15	1	44	148	10	108	9.19e-48	150
MS.gene61107.t1	MTR_7g114050	68.067	119	27	2	13	131	11	118	1.90e-46	147
MS.gene61107.t1	MTR_1g011750	57.258	124	51	2	7	129	10	132	4.85e-36	121
MS.gene61107.t1	MTR_1g011850	57.258	124	51	2	7	129	10	132	4.85e-36	121
MS.gene61107.t1	MTR_1g114050	57.143	119	49	2	10	127	10	127	1.54e-35	120
MS.gene61107.t1	MTR_1g071040	61.538	104	38	2	25	127	28	130	4.66e-34	116
MS.gene61107.t1	MTR_1g069770	52.174	92	43	1	44	134	9	100	2.97e-25	93.2
MS.gene61107.t1	MTR_7g010440	70.588	51	15	0	77	127	122	172	1.25e-16	74.7
MS.gene61107.t1	MTR_4g005910	74.419	43	11	0	86	128	4	46	1.92e-16	69.3
MS.gene61107.t1	MTR_4g048490	43.810	105	30	2	23	127	26	101	4.35e-16	69.7
MS.gene61107.t1	MTR_7g027610	41.818	110	38	3	19	127	10	94	4.09e-15	67.4
MS.gene61107.t1	MTR_0653s0030	53.704	54	25	0	55	108	44	97	1.32e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61107.t1	AT5G02560	82.781	151	23	2	1	148	1	151	2.41e-78	229
MS.gene61107.t1	AT5G27670	75.333	150	33	2	1	148	1	148	1.18e-74	219
MS.gene61107.t1	AT5G02560	71.429	175	23	3	1	148	1	175	2.25e-73	217
MS.gene61107.t1	AT5G59870	75.000	148	29	3	4	143	3	150	1.70e-71	211
MS.gene61107.t1	AT1G51060	79.464	112	23	0	18	129	11	122	3.28e-60	182
MS.gene61107.t1	AT1G08880	72.093	129	32	1	7	131	2	130	2.55e-59	181
MS.gene61107.t1	AT1G54690	73.600	125	29	1	11	131	6	130	1.08e-58	179
MS.gene61107.t1	AT4G27230	78.378	111	24	0	18	128	11	121	1.71e-58	178
MS.gene61107.t1	AT4G27230	78.378	111	24	0	18	128	11	121	1.71e-58	178
MS.gene61107.t1	AT3G20670	78.378	111	24	0	18	128	11	121	2.65e-58	177
MS.gene61107.t1	AT5G54640	73.109	119	32	0	18	136	11	129	1.06e-57	176
MS.gene61107.t1	AT2G38810	59.130	115	45	2	16	129	21	134	2.25e-36	122
MS.gene61107.t1	AT2G38810	59.130	115	45	2	16	129	21	134	2.25e-36	122
MS.gene61107.t1	AT2G38810	59.130	115	45	2	16	129	21	134	2.25e-36	122
MS.gene61107.t1	AT2G38810	59.130	115	45	2	16	129	21	134	2.25e-36	122
MS.gene61107.t1	AT1G52740	61.905	105	38	2	24	127	27	130	1.52e-34	117
MS.gene61107.t1	AT3G54560	59.813	107	41	2	24	129	29	134	3.98e-34	116
MS.gene61107.t1	AT3G54560	59.813	107	41	2	24	129	29	134	3.98e-34	116
MS.gene61107.t1	AT4G13570	47.826	92	46	2	31	121	28	118	7.54e-20	79.7
MS.gene61107.t1	AT4G13570	47.253	91	46	2	32	121	35	124	1.70e-19	79.0

Find 39 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGCCTTGTCAGTCTTCTT+AGG	0.306518	4.4:-42559115	None:intergenic
GACTGACAAGGCTGCTTCTA+AGG	0.317190	4.4:+42559123	MS.gene61107:CDS
CTTGTCACCGACTTCTTCCT+TGG	0.333455	4.4:-42558794	None:intergenic
TGCTGCTGAAGTTCTTGAAT+TGG	0.339223	4.4:+42558940	MS.gene61107:CDS
TCTTCTTAGGCAAGAGAATA+GGG	0.366947	4.4:-42559102	None:intergenic
AGAACTGCTGCTAGGTATAC+TGG	0.389575	4.4:-42558908	None:intergenic
TACGCTCAGCGTGTTGGAAC+AGG	0.407073	4.4:+42558881	MS.gene61107:CDS
GTCTTCTTAGGCAAGAGAAT+AGG	0.408927	4.4:-42559103	None:intergenic
AAGAATCAGATCTAAGCCTT+TGG	0.432647	4.4:-42559178	None:intergenic
TATCGACCGATTCTTCCAAC+TGG	0.433444	4.4:-42558842	None:intergenic
AAGAGAATAGGGTTAATGTT+TGG	0.433482	4.4:-42559091	None:intergenic
GGCATGTGTTGTTGGCTGTT+AGG	0.442957	4.4:+42559008	MS.gene61107:CDS
CAGTTCCCAGTTGGAAGAAT+CGG	0.451986	4.4:+42558836	MS.gene61107:CDS
GCTGAAGTTCTTGAATTGGC+TGG	0.461152	4.4:+42558944	MS.gene61107:CDS
AGCACAAAGACAACGAAGAA+AGG	0.469389	4.4:+42558749	MS.gene61107:CDS
TATTCCAAGGCATGTGTTGT+TGG	0.470522	4.4:+42559000	MS.gene61107:CDS
AAAGGAGCCGGTGGAAGGAA+AGG	0.476592	4.4:+42558767	MS.gene61107:CDS
TGGTCCTCCTTTCCTTCCAC+CGG	0.483261	4.4:-42558774	None:intergenic
GAGCTTGGTAAATTACTTGC+TGG	0.499780	4.4:+42559040	MS.gene61107:CDS
ACAGCCAACAACACATGCCT+TGG	0.515073	4.4:-42559004	None:intergenic
ATCGGTCGATACTTGAAGAA+AGG	0.519021	4.4:+42558854	MS.gene61107:CDS
GGAAGATACGCTCAGCGTGT+TGG	0.531317	4.4:+42558875	MS.gene61107:CDS
GCCGGTCTTCAGTTCCCAGT+TGG	0.545988	4.4:+42558827	MS.gene61107:CDS
CGAAGAAAGGAGCCGGTGGA+AGG	0.551864	4.4:+42558762	MS.gene61107:CDS
GTTAGGAACGATGAAGAGCT+TGG	0.552797	4.4:+42559025	MS.gene61107:CDS
TCCAACTGGGAACTGAAGAC+CGG	0.563850	4.4:-42558828	None:intergenic
GCTGGTGTTACAATTGCTCA+TGG	0.569274	4.4:+42559058	MS.gene61107:CDS
GATATTCAAGAACTGCTGCT+AGG	0.592671	4.4:-42558916	None:intergenic
AAGACAACGAAGAAAGGAGC+CGG	0.594835	4.4:+42558755	MS.gene61107:CDS
GTGACAAGATCAATCAGAGC+CGG	0.601599	4.4:+42558809	MS.gene61107:CDS
AGAAGAATAGAATTATTCCA+AGG	0.617592	4.4:+42558987	MS.gene61107:CDS
AAAAGCTGGAAAGTCTCCAA+AGG	0.624560	4.4:+42559162	MS.gene61107:CDS
GGAGCCGGTGGAAGGAAAGG+AGG	0.629948	4.4:+42558770	MS.gene61107:CDS
ACAACGAAGAAAGGAGCCGG+TGG	0.637849	4.4:+42558758	MS.gene61107:CDS
AGGAGGACCAAGGAAGAAGT+CGG	0.642719	4.4:+42558787	MS.gene61107:CDS
ATCGACCGATTCTTCCAACT+GGG	0.643258	4.4:-42558841	None:intergenic
CTTGCCTAAGAAGACTGACA+AGG	0.657442	4.4:+42559111	MS.gene61107:CDS
GTGGAAGGAAAGGAGGACCA+AGG	0.686614	4.4:+42558777	MS.gene61107:CDS
GGTGTTACAATTGCTCATGG+TGG	0.699701	4.4:+42559061	MS.gene61107:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAAGAATAGAATTATTCCA+AGG	+	chr4.4:42558987-42559006	MS.gene61107:CDS	25.0%
	AAGAGAATAGGGTTAATGTT+TGG	-	chr4.4:42559094-42559113	None:intergenic	30.0%
	TCTTCTTAGGCAAGAGAATA+GGG	-	chr4.4:42559105-42559124	None:intergenic	35.0%
	AAAAGCTGGAAAGTCTCCAA+AGG	+	chr4.4:42559162-42559181	MS.gene61107:CDS	40.0%
	AGCACAAAGACAACGAAGAA+AGG	+	chr4.4:42558749-42558768	MS.gene61107:CDS	40.0%
	ATCGGTCGATACTTGAAGAA+AGG	+	chr4.4:42558854-42558873	MS.gene61107:CDS	40.0%
	GAGCTTGGTAAATTACTTGC+TGG	+	chr4.4:42559040-42559059	MS.gene61107:CDS	40.0%
	GATATTCAAGAACTGCTGCT+AGG	-	chr4.4:42558919-42558938	None:intergenic	40.0%
	GTCTTCTTAGGCAAGAGAAT+AGG	-	chr4.4:42559106-42559125	None:intergenic	40.0%
	TATTCCAAGGCATGTGTTGT+TGG	+	chr4.4:42559000-42559019	MS.gene61107:CDS	40.0%
	TGCTGCTGAAGTTCTTGAAT+TGG	+	chr4.4:42558940-42558959	MS.gene61107:CDS	40.0%
!!!	GGAGACTTTCCAGCTTTTTT+GGG	-	chr4.4:42559160-42559179	None:intergenic	40.0%
!!!	TGGAGACTTTCCAGCTTTTT+TGG	-	chr4.4:42559161-42559180	None:intergenic	40.0%
	AAGACAACGAAGAAAGGAGC+CGG	+	chr4.4:42558755-42558774	MS.gene61107:CDS	45.0%
	AGAACTGCTGCTAGGTATAC+TGG	-	chr4.4:42558911-42558930	None:intergenic	45.0%
	CAGTTCCCAGTTGGAAGAAT+CGG	+	chr4.4:42558836-42558855	MS.gene61107:CDS	45.0%
	CCTAAGTCTCCCAAAAAAGC+TGG	+	chr4.4:42559148-42559167	MS.gene61107:CDS	45.0%
	CTTGCCTAAGAAGACTGACA+AGG	+	chr4.4:42559111-42559130	MS.gene61107:CDS	45.0%
	GCTGAAGTTCTTGAATTGGC+TGG	+	chr4.4:42558944-42558963	MS.gene61107:CDS	45.0%
	GTGACAAGATCAATCAGAGC+CGG	+	chr4.4:42558809-42558828	MS.gene61107:CDS	45.0%
	GTTAGGAACGATGAAGAGCT+TGG	+	chr4.4:42559025-42559044	MS.gene61107:CDS	45.0%
!	ATCGACCGATTCTTCCAACT+GGG	-	chr4.4:42558844-42558863	None:intergenic	45.0%
!	GGTGTTACAATTGCTCATGG+TGG	+	chr4.4:42559061-42559080	MS.gene61107:CDS	45.0%
!	TATCGACCGATTCTTCCAAC+TGG	-	chr4.4:42558845-42558864	None:intergenic	45.0%
!!	GCTGGTGTTACAATTGCTCA+TGG	+	chr4.4:42559058-42559077	MS.gene61107:CDS	45.0%
!!!	CCAGCTTTTTTGGGAGACTT+AGG	-	chr4.4:42559151-42559170	None:intergenic	45.0%
	ACAGCCAACAACACATGCCT+TGG	-	chr4.4:42559007-42559026	None:intergenic	50.0%
	AGGAGGACCAAGGAAGAAGT+CGG	+	chr4.4:42558787-42558806	MS.gene61107:CDS	50.0%
	GACTGACAAGGCTGCTTCTA+AGG	+	chr4.4:42559123-42559142	MS.gene61107:CDS	50.0%
	TCCAACTGGGAACTGAAGAC+CGG	-	chr4.4:42558831-42558850	None:intergenic	50.0%
!	GCAGCCTTGTCAGTCTTCTT+AGG	-	chr4.4:42559118-42559137	None:intergenic	50.0%
!	GGCATGTGTTGTTGGCTGTT+AGG	+	chr4.4:42559008-42559027	MS.gene61107:CDS	50.0%
!!	CTTGTCACCGACTTCTTCCT+TGG	-	chr4.4:42558797-42558816	None:intergenic	50.0%
	AAAGGAGCCGGTGGAAGGAA+AGG	+	chr4.4:42558767-42558786	MS.gene61107:CDS	55.0%
	ACAACGAAGAAAGGAGCCGG+TGG	+	chr4.4:42558758-42558777	MS.gene61107:CDS	55.0%
	GGAAGATACGCTCAGCGTGT+TGG	+	chr4.4:42558875-42558894	MS.gene61107:CDS	55.0%
	GTGGAAGGAAAGGAGGACCA+AGG	+	chr4.4:42558777-42558796	MS.gene61107:CDS	55.0%
	TACGCTCAGCGTGTTGGAAC+AGG	+	chr4.4:42558881-42558900	MS.gene61107:CDS	55.0%
	TGGTCCTCCTTTCCTTCCAC+CGG	-	chr4.4:42558777-42558796	None:intergenic	55.0%
	CGAAGAAAGGAGCCGGTGGA+AGG	+	chr4.4:42558762-42558781	MS.gene61107:CDS	60.0%
	GCCGGTCTTCAGTTCCCAGT+TGG	+	chr4.4:42558827-42558846	MS.gene61107:CDS	60.0%
	GGAGCCGGTGGAAGGAAAGG+AGG	+	chr4.4:42558770-42558789	MS.gene61107:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	42558740	42559189	42558740	ID=MS.gene61107
chr4.4	mRNA	42558740	42559189	42558740	ID=MS.gene61107.t1;Parent=MS.gene61107
chr4.4	exon	42558740	42559189	42558740	ID=MS.gene61107.t1.exon1;Parent=MS.gene61107.t1
chr4.4	CDS	42558740	42559189	42558740	ID=cds.MS.gene61107.t1;Parent=MS.gene61107.t1

Gene Sequence

>MS.gene61107

ATGGACGCAAGCACAAAGACAACGAAGAAAGGAGCCGGTGGAAGGAAAGGAGGACCAAGGAAGAAGTCGGTGACAAGATCAATCAGAGCCGGTCTTCAGTTCCCAGTTGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATACGCTCAGCGTGTTGGAACAGGTGCACCAGTATACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATTATTCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGTAAATTACTTGCTGGTGTTACAATTGCTCATGGTGGTGTTCTTCCAAACATTAACCCTATTCTCTTGCCTAAGAAGACTGACAAGGCTGCTTCTAAGGAACCTAAGTCTCCCAAAAAAGCTGGAAAGTCTCCAAAGGCTTAG

Protein sequence

>MS.gene61107.t1

MDASTKTTKKGAGGRKGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTDKAASKEPKSPKKAGKSPKA