Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61659.t1 | XP_013455742.1 | 97 | 471 | 14 | 0 | 28 | 498 | 1 | 471 | 1.90E-253 | 884.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61659.t1 | Q9SJE8 | 53.8 | 437 | 195 | 2 | 56 | 489 | 1 | 433 | 3.0e-127 | 456.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61659.t1 | A0A072UL02 | 97.0 | 471 | 14 | 0 | 28 | 498 | 1 | 471 | 1.3e-253 | 884.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07488 | MS.gene61659 | PPI |
| MS.gene61659 | MS.gene07489 | PPI |
| MS.gene61659 | MS.gene044738 | PPI |
| MS.gene004961 | MS.gene61659 | PPI |
| MS.gene062179 | MS.gene61659 | PPI |
| MS.gene96117 | MS.gene61659 | PPI |
| MS.gene004960 | MS.gene61659 | PPI |
| MS.gene61659 | MS.gene66665 | PPI |
| MS.gene062180 | MS.gene61659 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61659.t1 | MTR_4g051575 | 97.028 | 471 | 14 | 0 | 28 | 498 | 1 | 471 | 0.0 | 946 |
| MS.gene61659.t1 | MTR_4g051575 | 96.418 | 335 | 11 | 1 | 164 | 498 | 4 | 337 | 0.0 | 663 |
| MS.gene61659.t1 | MTR_2g087288 | 48.796 | 457 | 224 | 4 | 33 | 486 | 2 | 451 | 1.48e-156 | 453 |
| MS.gene61659.t1 | MTR_7g106260 | 50.439 | 456 | 217 | 4 | 37 | 489 | 11 | 460 | 1.18e-154 | 449 |
| MS.gene61659.t1 | MTR_2g087285 | 48.946 | 427 | 206 | 4 | 28 | 450 | 1 | 419 | 1.45e-143 | 420 |
| MS.gene61659.t1 | MTR_1g077670 | 43.765 | 425 | 217 | 5 | 62 | 466 | 43 | 465 | 4.63e-117 | 353 |
| MS.gene61659.t1 | MTR_1g077660 | 45.885 | 401 | 193 | 5 | 62 | 441 | 37 | 434 | 1.12e-115 | 349 |
| MS.gene61659.t1 | MTR_1g115140 | 42.149 | 363 | 192 | 3 | 46 | 405 | 26 | 373 | 1.03e-91 | 290 |
| MS.gene61659.t1 | MTR_4g098570 | 40.909 | 396 | 222 | 4 | 14 | 406 | 3 | 389 | 3.68e-91 | 288 |
| MS.gene61659.t1 | MTR_5g014310 | 40.731 | 383 | 221 | 4 | 29 | 406 | 17 | 398 | 1.59e-90 | 287 |
| MS.gene61659.t1 | MTR_1g115175 | 41.436 | 362 | 202 | 3 | 47 | 406 | 23 | 376 | 8.97e-89 | 282 |
| MS.gene61659.t1 | MTR_7g094770 | 37.755 | 392 | 236 | 4 | 64 | 450 | 51 | 439 | 4.51e-87 | 275 |
| MS.gene61659.t1 | MTR_4g098610 | 39.798 | 397 | 225 | 5 | 14 | 406 | 3 | 389 | 2.62e-82 | 265 |
| MS.gene61659.t1 | MTR_1g115190 | 41.209 | 364 | 203 | 3 | 46 | 406 | 23 | 378 | 8.01e-82 | 264 |
| MS.gene61659.t1 | MTR_8g104130 | 33.131 | 329 | 213 | 5 | 56 | 382 | 47 | 370 | 4.33e-59 | 204 |
| MS.gene61659.t1 | MTR_8g104110 | 32.258 | 341 | 217 | 6 | 54 | 382 | 49 | 387 | 1.30e-58 | 203 |
| MS.gene61659.t1 | MTR_1g111250 | 33.129 | 326 | 206 | 4 | 68 | 384 | 74 | 396 | 7.28e-57 | 198 |
| MS.gene61659.t1 | MTR_6g084680 | 31.507 | 365 | 236 | 5 | 31 | 382 | 44 | 407 | 5.05e-56 | 197 |
| MS.gene61659.t1 | MTR_8g104120 | 32.923 | 325 | 205 | 5 | 68 | 382 | 60 | 381 | 2.39e-51 | 184 |
| MS.gene61659.t1 | MTR_0710s0020 | 47.619 | 168 | 81 | 1 | 46 | 213 | 23 | 183 | 4.66e-39 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61659.t1 | AT1G08440 | 57.614 | 394 | 164 | 1 | 56 | 446 | 1 | 394 | 7.01e-171 | 491 |
| MS.gene61659.t1 | AT2G27240 | 53.725 | 443 | 196 | 3 | 56 | 493 | 1 | 439 | 5.96e-170 | 488 |
| MS.gene61659.t1 | AT2G27240 | 50.959 | 469 | 195 | 5 | 56 | 493 | 1 | 465 | 2.70e-164 | 474 |
| MS.gene61659.t1 | AT1G08430 | 49.558 | 452 | 215 | 4 | 56 | 498 | 1 | 448 | 3.41e-157 | 456 |
| MS.gene61659.t1 | AT3G11680 | 49.561 | 456 | 216 | 7 | 45 | 489 | 13 | 465 | 5.81e-145 | 425 |
| MS.gene61659.t1 | AT3G11680 | 49.561 | 456 | 216 | 7 | 45 | 489 | 15 | 467 | 6.76e-145 | 425 |
| MS.gene61659.t1 | AT4G00910 | 40.816 | 441 | 234 | 3 | 53 | 467 | 43 | 482 | 1.39e-115 | 349 |
| MS.gene61659.t1 | AT4G17970 | 42.382 | 361 | 196 | 2 | 46 | 406 | 28 | 376 | 1.42e-93 | 295 |
| MS.gene61659.t1 | AT5G46600 | 41.620 | 358 | 202 | 2 | 55 | 406 | 29 | 385 | 1.34e-89 | 284 |
| MS.gene61659.t1 | AT5G46610 | 41.690 | 355 | 203 | 2 | 55 | 406 | 28 | 381 | 6.84e-89 | 282 |
| MS.gene61659.t1 | AT5G46610 | 38.028 | 355 | 192 | 3 | 55 | 406 | 28 | 357 | 5.80e-75 | 245 |
| MS.gene61659.t1 | AT5G46610 | 35.775 | 355 | 177 | 3 | 55 | 406 | 28 | 334 | 1.72e-67 | 225 |
| MS.gene61659.t1 | AT3G18440 | 33.636 | 330 | 208 | 4 | 62 | 382 | 73 | 400 | 3.73e-63 | 216 |
| MS.gene61659.t1 | AT2G17470 | 31.006 | 358 | 233 | 6 | 60 | 406 | 21 | 375 | 4.64e-57 | 198 |
| MS.gene61659.t1 | AT2G17470 | 31.006 | 358 | 233 | 6 | 60 | 406 | 43 | 397 | 9.03e-57 | 198 |
| MS.gene61659.t1 | AT1G68600 | 32.923 | 325 | 207 | 4 | 69 | 384 | 49 | 371 | 9.46e-57 | 197 |
| MS.gene61659.t1 | AT1G18420 | 30.919 | 359 | 234 | 6 | 55 | 402 | 70 | 425 | 1.67e-56 | 198 |
| MS.gene61659.t1 | AT1G18420 | 31.672 | 341 | 219 | 6 | 73 | 402 | 5 | 342 | 3.14e-56 | 195 |
| MS.gene61659.t1 | AT1G25480 | 32.378 | 349 | 224 | 5 | 68 | 407 | 59 | 404 | 3.82e-56 | 196 |
| MS.gene61659.t1 | AT4G17585 | 42.466 | 73 | 31 | 1 | 46 | 118 | 28 | 89 | 4.27e-12 | 64.7 |
Find 69 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTGCTTCAATCAAGTTTA+TGG | 0.032989 | 4.1:-46795834 | MS.gene61659:CDS |
| CATTACTTGGCTAGCTTATC+TGG | 0.169349 | 4.1:-46797571 | MS.gene61659:CDS |
| CAACCACTTTATGAAAATTT+TGG | 0.182547 | 4.1:-46798405 | MS.gene61659:CDS |
| TGGTTCCCATCTTGCAAAAT+TGG | 0.218158 | 4.1:+46796468 | None:intergenic |
| TGGAATCAATACCTTAAAAT+TGG | 0.229282 | 4.1:-46796415 | MS.gene61659:CDS |
| ACATTTCTGTGCATAGTTCT+TGG | 0.240949 | 4.1:+46795974 | None:intergenic |
| TTCTCTTTAATATCTGTATC+TGG | 0.259432 | 4.1:-46797311 | MS.gene61659:CDS |
| GTGAAATCAAGATATGATTA+TGG | 0.273521 | 4.1:-46797356 | MS.gene61659:CDS |
| ACAGGGGAACCAATTCTAAT+TGG | 0.279623 | 4.1:-46797544 | MS.gene61659:CDS |
| TCATATCTTGATTTCACTTT+GGG | 0.284806 | 4.1:+46797362 | None:intergenic |
| TTCTTCTAGTGCATACAATA+TGG | 0.289816 | 4.1:-46798631 | MS.gene61659:intron |
| GCTTTCTCTTGGTCCATGTT+TGG | 0.290756 | 4.1:+46798603 | None:intergenic |
| GGAGATTGTGACACTTCTTT+AGG | 0.293984 | 4.1:+46795661 | None:intergenic |
| GCATTCGCAACGTGAGTTTC+CGG | 0.306704 | 4.1:+46795883 | None:intergenic |
| CATGTGTATTAGTTAGAGTA+TGG | 0.315903 | 4.1:-46798581 | MS.gene61659:CDS |
| ACATTGGTAGCTGGTGCTTT+AGG | 0.317161 | 4.1:-46797604 | MS.gene61659:CDS |
| AGTTGGACTTGCAATCTCTT+TGG | 0.318396 | 4.1:-46798448 | MS.gene61659:CDS |
| TAGCTGGTGCTTTAGGTGTT+GGG | 0.321949 | 4.1:-46797597 | MS.gene61659:CDS |
| ACATGTGAATTCTCAATCTT+AGG | 0.327576 | 4.1:+46795613 | None:intergenic |
| ATACTTGGTAACTTCTTAGA+AGG | 0.349508 | 4.1:-46797140 | MS.gene61659:intron |
| AGGTGTTGGGGCTCATTACT+TGG | 0.372554 | 4.1:-46797584 | MS.gene61659:CDS |
| ACAAAGAATCCAATTAGAAT+TGG | 0.373993 | 4.1:+46797535 | None:intergenic |
| CTTTAGGAAAAGGTTTGAAT+AGG | 0.382546 | 4.1:-46797636 | MS.gene61659:CDS |
| TTGATTGAAGCAACGAGTTA+AGG | 0.386024 | 4.1:+46795842 | None:intergenic |
| ACCTTCTTCATAAGAACCTT+TGG | 0.391062 | 4.1:+46798540 | None:intergenic |
| ATCATATCTTGATTTCACTT+TGG | 0.405404 | 4.1:+46797361 | None:intergenic |
| TGAAATCAAGATATGATTAT+GGG | 0.418681 | 4.1:-46797355 | MS.gene61659:CDS |
| TCCAAAGGTTCTTATGAAGA+AGG | 0.419353 | 4.1:-46798541 | MS.gene61659:CDS |
| GCTTTCAAGTTGGAAATACT+TGG | 0.421104 | 4.1:-46797155 | MS.gene61659:CDS |
| GTAGCTGGTGCTTTAGGTGT+TGG | 0.424814 | 4.1:-46797598 | MS.gene61659:CDS |
| TTAGGAGCTACTTTAGGAAA+AGG | 0.426037 | 4.1:-46797646 | MS.gene61659:intron |
| AGTTCAAATTTCGTCACCCT+TGG | 0.430578 | 4.1:-46796435 | MS.gene61659:CDS |
| GTGATTCATTCACTAAAAGT+TGG | 0.434830 | 4.1:-46798465 | MS.gene61659:CDS |
| GCTAGCTTATCTGGAGAGAC+AGG | 0.435266 | 4.1:-46797562 | MS.gene61659:CDS |
| AAGCTTCTCGTGCACCTTCG+AGG | 0.443711 | 4.1:-46807057 | MS.gene61659:intron |
| GAATTCTCAATCTTAGGATC+TGG | 0.446590 | 4.1:+46795619 | None:intergenic |
| CTAATACACATGCTTTCTCT+TGG | 0.459956 | 4.1:+46798592 | None:intergenic |
| AACTCTTCACCAGCCCAAAC+AGG | 0.473547 | 4.1:+46797191 | None:intergenic |
| TGATTCAATTGCTTTCAAGT+TGG | 0.485163 | 4.1:-46797165 | MS.gene61659:CDS |
| TGATGATGAAGTATTACAAA+TGG | 0.486318 | 4.1:-46797282 | MS.gene61659:CDS |
| AGCTGGTGCTTTAGGTGTTG+GGG | 0.490031 | 4.1:-46797596 | MS.gene61659:CDS |
| TTTGTTTGTCCTGTTTGGGC+TGG | 0.492028 | 4.1:-46797200 | MS.gene61659:CDS |
| ATATCGAATGGAAGCTCTAA+AGG | 0.501202 | 4.1:-46796371 | MS.gene61659:CDS |
| GGCAGTGATGACTGTGGTGG+TGG | 0.502115 | 4.1:-46798367 | MS.gene61659:CDS |
| CTTTGTGCGGATTATCGCAC+AGG | 0.502428 | 4.1:+46807077 | None:intergenic |
| TATTACAAATGGCTCATAAG+AGG | 0.504629 | 4.1:-46797271 | MS.gene61659:CDS |
| GGATTCTTTGTATTTCTACA+AGG | 0.514172 | 4.1:-46797523 | MS.gene61659:intron |
| CAATATGGTGTCTCCAAACA+TGG | 0.518606 | 4.1:-46798616 | MS.gene61659:CDS |
| TAAAAGCAATGATGAGTCAC+TGG | 0.520604 | 4.1:-46796589 | MS.gene61659:intron |
| GGTTCAGCACATTCACAAGT+TGG | 0.522133 | 4.1:+46795640 | None:intergenic |
| AATTTGAACTTGCCATGACC+TGG | 0.528501 | 4.1:+46796448 | None:intergenic |
| AATGAAGCAACTGTCGCAGC+TGG | 0.536056 | 4.1:+46795784 | None:intergenic |
| GAATAGGACAATGGCAACAT+TGG | 0.537381 | 4.1:-46797620 | MS.gene61659:CDS |
| GCAAGATGGGAACCAGGTCA+TGG | 0.553785 | 4.1:-46796460 | MS.gene61659:CDS |
| ACAATGGCAACATTGGTAGC+TGG | 0.571785 | 4.1:-46797613 | MS.gene61659:CDS |
| TCGTCAATGTGCATATCGAA+TGG | 0.577376 | 4.1:-46796383 | MS.gene61659:CDS |
| ATGTGTATTAGTTAGAGTAT+GGG | 0.595419 | 4.1:-46798580 | MS.gene61659:CDS |
| CTAGCTTATCTGGAGAGACA+GGG | 0.601883 | 4.1:-46797561 | MS.gene61659:CDS |
| AATGACAAGAAACTCATCAC+CGG | 0.615723 | 4.1:-46795902 | MS.gene61659:CDS |
| AAAAGGTTTGAATAGGACAA+TGG | 0.617027 | 4.1:-46797629 | MS.gene61659:CDS |
| AATGTGTTTAGAATCAAGCA+TGG | 0.622652 | 4.1:-46795956 | MS.gene61659:CDS |
| TGAGTATTTCAAAACAACAA+AGG | 0.637507 | 4.1:-46796670 | MS.gene61659:CDS |
| CTCAGAGCATAACACCTCGA+AGG | 0.652381 | 4.1:+46807043 | None:intergenic |
| TAGCTTATCTGGAGAGACAG+GGG | 0.659742 | 4.1:-46797560 | MS.gene61659:CDS |
| GTGGGCAGTGATGACTGTGG+TGG | 0.678035 | 4.1:-46798370 | MS.gene61659:CDS |
| GGTGCACGAGAAGCTTTGTG+CGG | 0.694652 | 4.1:+46807064 | None:intergenic |
| ACAATGAGAAGAATCAAACA+AGG | 0.729534 | 4.1:-46805709 | MS.gene61659:intron |
| TGATATTGTTGATTGCACTG+AGG | 0.767820 | 4.1:-46795755 | MS.gene61659:CDS |
| AATGTGGGCAGTGATGACTG+TGG | 0.817988 | 4.1:-46798373 | MS.gene61659:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 46795564 | 46807104 | 46795564 | ID=MS.gene61659 |
| chr4.1 | mRNA | 46795564 | 46807104 | 46795564 | ID=MS.gene61659.t1;Parent=MS.gene61659 |
| chr4.1 | exon | 46807058 | 46807104 | 46807058 | ID=MS.gene61659.t1.exon1;Parent=MS.gene61659.t1 |
| chr4.1 | CDS | 46807058 | 46807104 | 46807058 | ID=cds.MS.gene61659.t1;Parent=MS.gene61659.t1 |
| chr4.1 | exon | 46805710 | 46805733 | 46805710 | ID=MS.gene61659.t1.exon2;Parent=MS.gene61659.t1 |
| chr4.1 | CDS | 46805710 | 46805733 | 46805710 | ID=cds.MS.gene61659.t1;Parent=MS.gene61659.t1 |
| chr4.1 | exon | 46798349 | 46798644 | 46798349 | ID=MS.gene61659.t1.exon3;Parent=MS.gene61659.t1 |
| chr4.1 | CDS | 46798349 | 46798644 | 46798349 | ID=cds.MS.gene61659.t1;Parent=MS.gene61659.t1 |
| chr4.1 | exon | 46797524 | 46797664 | 46797524 | ID=MS.gene61659.t1.exon4;Parent=MS.gene61659.t1 |
| chr4.1 | CDS | 46797524 | 46797664 | 46797524 | ID=cds.MS.gene61659.t1;Parent=MS.gene61659.t1 |
| chr4.1 | exon | 46797141 | 46797413 | 46797141 | ID=MS.gene61659.t1.exon5;Parent=MS.gene61659.t1 |
| chr4.1 | CDS | 46797141 | 46797413 | 46797141 | ID=cds.MS.gene61659.t1;Parent=MS.gene61659.t1 |
| chr4.1 | exon | 46796590 | 46796702 | 46796590 | ID=MS.gene61659.t1.exon6;Parent=MS.gene61659.t1 |
| chr4.1 | CDS | 46796590 | 46796702 | 46796590 | ID=cds.MS.gene61659.t1;Parent=MS.gene61659.t1 |
| chr4.1 | exon | 46796348 | 46796491 | 46796348 | ID=MS.gene61659.t1.exon7;Parent=MS.gene61659.t1 |
| chr4.1 | CDS | 46796348 | 46796491 | 46796348 | ID=cds.MS.gene61659.t1;Parent=MS.gene61659.t1 |
| chr4.1 | exon | 46795564 | 46796022 | 46795564 | ID=MS.gene61659.t1.exon8;Parent=MS.gene61659.t1 |
| chr4.1 | CDS | 46795564 | 46796022 | 46795564 | ID=cds.MS.gene61659.t1;Parent=MS.gene61659.t1 |
| Gene Sequence |
| Protein sequence |
