Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62991.t1 | XP_013466508.1 | 96.9 | 192 | 6 | 0 | 1 | 192 | 1 | 192 | 4.00E-95 | 357.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62991.t1 | Q6NLC8 | 45.8 | 201 | 95 | 5 | 1 | 188 | 1 | 200 | 1.1e-32 | 141.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62991.t1 | A0A072VEV4 | 96.9 | 192 | 6 | 0 | 1 | 192 | 1 | 192 | 2.9e-95 | 357.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050521 | MS.gene62991 | 0.836911 | 7.09E-57 | -1.69E-46 |
| MS.gene053957 | MS.gene62991 | 0.802719 | 4.91E-49 | -1.69E-46 |
| MS.gene058015 | MS.gene62991 | 0.836106 | 1.14E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene62991 | MS.gene008202 | PPI |
| MS.gene62235 | MS.gene62991 | PPI |
| MS.gene62991 | MS.gene04894 | PPI |
| MS.gene017455 | MS.gene62991 | PPI |
| MS.gene63347 | MS.gene62991 | PPI |
| MS.gene000732 | MS.gene62991 | PPI |
| MS.gene29468 | MS.gene62991 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62991.t1 | MTR_1g030740 | 96.875 | 192 | 6 | 0 | 1 | 192 | 1 | 192 | 4.29e-134 | 374 |
| MS.gene62991.t1 | MTR_4g085100 | 70.857 | 175 | 43 | 4 | 1 | 170 | 1 | 172 | 5.36e-77 | 229 |
| MS.gene62991.t1 | MTR_5g079610 | 54.070 | 172 | 69 | 4 | 1 | 164 | 1 | 170 | 1.62e-55 | 175 |
| MS.gene62991.t1 | MTR_3g067730 | 49.746 | 197 | 84 | 4 | 1 | 182 | 1 | 197 | 1.07e-52 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62991.t1 | AT2G01340 | 63.529 | 170 | 57 | 3 | 1 | 169 | 1 | 166 | 4.11e-64 | 197 |
| MS.gene62991.t1 | AT1G71015 | 57.576 | 165 | 64 | 3 | 1 | 163 | 1 | 161 | 7.27e-49 | 157 |
| MS.gene62991.t1 | AT1G71015 | 57.576 | 165 | 64 | 3 | 1 | 163 | 1 | 161 | 7.27e-49 | 157 |
| MS.gene62991.t1 | AT1G66480 | 50.000 | 176 | 74 | 4 | 1 | 163 | 1 | 175 | 2.12e-46 | 152 |
| MS.gene62991.t1 | AT5G37840 | 42.132 | 197 | 104 | 6 | 1 | 191 | 1 | 193 | 1.75e-39 | 134 |
Find 40 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAATAATGGGAAATAGTTT+AGG | 0.332015 | 1.3:+16528924 | None:intergenic |
| TCTGAGGAAGTGAAGCATTA+TGG | 0.340674 | 1.3:+16529059 | MS.gene62991:CDS |
| TAATGATAAGAAAATGAAAA+AGG | 0.345766 | 1.3:+16529430 | MS.gene62991:CDS |
| CCCACCTCGAAGTCGTTCAT+TGG | 0.353620 | 1.3:-16529269 | None:intergenic |
| TCTTGTAACTCGACTTTCCT+TGG | 0.360185 | 1.3:-16529153 | None:intergenic |
| AACTTCTCAACATCTGCCTT+TGG | 0.375562 | 1.3:-16529338 | None:intergenic |
| AACTCTTTGTGTGCTTCCAA+TGG | 0.426840 | 1.3:-16529095 | None:intergenic |
| GAAAGCCGGTGAAGTGCTTA+AGG | 0.427598 | 1.3:+16529010 | MS.gene62991:CDS |
| GTTACAAGAAGGGTTCGTTC+GGG | 0.451959 | 1.3:+16529167 | MS.gene62991:CDS |
| CTTGTAACTCGACTTTCCTT+GGG | 0.453744 | 1.3:-16529152 | None:intergenic |
| AGTTACAAGAAGGGTTCGTT+CGG | 0.464996 | 1.3:+16529166 | MS.gene62991:CDS |
| AAGAAGAGTTGACGAATGGC+GGG | 0.488902 | 1.3:+16529294 | MS.gene62991:CDS |
| AGCACTTCACCGGCTTTCAC+CGG | 0.493553 | 1.3:-16529005 | None:intergenic |
| GTGATCCTTAAGCACTTCAC+CGG | 0.518764 | 1.3:-16529015 | None:intergenic |
| ACAAAGGTGATGAAAATTGA+TGG | 0.530975 | 1.3:+16528960 | MS.gene62991:CDS |
| TCATTGGTTTCATAATCGTA+AGG | 0.535977 | 1.3:-16529253 | None:intergenic |
| AGTTGACGAATGGCGGGGTG+AGG | 0.542180 | 1.3:+16529300 | MS.gene62991:CDS |
| CTAAAGACACCGGTGAAAGC+CGG | 0.542691 | 1.3:+16528996 | MS.gene62991:CDS |
| TCTTGTCTTGTTAGAATCTG+AGG | 0.544333 | 1.3:+16529043 | MS.gene62991:CDS |
| TGGAAGGGTAGCAGAGGAGT+TGG | 0.545103 | 1.3:+16529461 | MS.gene62991:CDS |
| GAAACCAATGAACGACTTCG+AGG | 0.561915 | 1.3:+16529265 | MS.gene62991:CDS |
| AGGAAAGTCGAGTTACAAGA+AGG | 0.564448 | 1.3:+16529156 | MS.gene62991:CDS |
| ATAATGGGAAATAGTTTAGG+TGG | 0.567511 | 1.3:+16528927 | None:intergenic |
| GAAGATTATGAGTCTTTGCA+TGG | 0.572826 | 1.3:+16529397 | MS.gene62991:CDS |
| TGGAGTGAGAGCGAAGCCAT+TGG | 0.574068 | 1.3:+16529079 | MS.gene62991:CDS |
| AAAGAAGAGTTGACGAATGG+CGG | 0.576273 | 1.3:+16529293 | MS.gene62991:CDS |
| GATTCTAACAAGACAAGACC+AGG | 0.591028 | 1.3:-16529038 | None:intergenic |
| ACCAATGAACGACTTCGAGG+TGG | 0.613366 | 1.3:+16529268 | MS.gene62991:CDS |
| GATGCAAAGTAGTAAAGATG+AGG | 0.620365 | 1.3:+16529361 | MS.gene62991:CDS |
| AGAGTCTGTCTTTAGCTCGA+AGG | 0.625951 | 1.3:+16529219 | MS.gene62991:CDS |
| CCAATGAACGACTTCGAGGT+GGG | 0.626451 | 1.3:+16529269 | MS.gene62991:CDS |
| TTACAAGAAGGGTTCGTTCG+GGG | 0.631852 | 1.3:+16529168 | MS.gene62991:CDS |
| ATGCATTGGAAGGGTAGCAG+AGG | 0.643927 | 1.3:+16529455 | MS.gene62991:CDS |
| GGAAAGTCGAGTTACAAGAA+GGG | 0.646808 | 1.3:+16529157 | MS.gene62991:CDS |
| GGAAAAGAAGAGTTGACGAA+TGG | 0.648290 | 1.3:+16529290 | MS.gene62991:CDS |
| AGAAGAGTTGACGAATGGCG+GGG | 0.660824 | 1.3:+16529295 | MS.gene62991:CDS |
| GTTGAAAATGAGACTTCCAA+AGG | 0.662765 | 1.3:+16529322 | MS.gene62991:CDS |
| AACATTCAAGCTAAAGACAC+CGG | 0.668856 | 1.3:+16528986 | MS.gene62991:CDS |
| ATTCTAACAAGACAAGACCA+GGG | 0.767432 | 1.3:-16529037 | None:intergenic |
| GAAGTGCTTAAGGATCACCC+TGG | 0.784850 | 1.3:+16529020 | MS.gene62991:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGATAAGAAAATGAAAA+AGG | + | chr1.3:16529430-16529449 | MS.gene62991:CDS | 15.0% |
| ! | AAATGAAAAAGGAAATGCAT+TGG | + | chr1.3:16529441-16529460 | MS.gene62991:CDS | 25.0% |
| ! | TGAATACAAAAAAGCTTATG+AGG | + | chr1.3:16529484-16529503 | MS.gene62991:CDS | 25.0% |
| !! | AAAAAGACTCTATTTTCTTG+TGG | + | chr1.3:16529124-16529143 | MS.gene62991:CDS | 25.0% |
| !! | ACAAGAAAATAGAGTCTTTT+TGG | - | chr1.3:16529125-16529144 | None:intergenic | 25.0% |
| AAAAAGGAAATGCATTGGAA+GGG | + | chr1.3:16529446-16529465 | MS.gene62991:CDS | 30.0% | |
| ACAAAGGTGATGAAAATTGA+TGG | + | chr1.3:16528960-16528979 | MS.gene62991:CDS | 30.0% | |
| AGGTGGAAAAAAATCTACAA+AGG | + | chr1.3:16528944-16528963 | MS.gene62991:CDS | 30.0% | |
| !! | TCATTGGTTTCATAATCGTA+AGG | - | chr1.3:16529256-16529275 | None:intergenic | 30.0% |
| AACATTCAAGCTAAAGACAC+CGG | + | chr1.3:16528986-16529005 | MS.gene62991:CDS | 35.0% | |
| ATTCTAACAAGACAAGACCA+GGG | - | chr1.3:16529040-16529059 | None:intergenic | 35.0% | |
| GAAAAAGGAAATGCATTGGA+AGG | + | chr1.3:16529445-16529464 | MS.gene62991:CDS | 35.0% | |
| GAAGATTATGAGTCTTTGCA+TGG | + | chr1.3:16529397-16529416 | MS.gene62991:CDS | 35.0% | |
| GATGCAAAGTAGTAAAGATG+AGG | + | chr1.3:16529361-16529380 | MS.gene62991:CDS | 35.0% | |
| GTTGAAAATGAGACTTCCAA+AGG | + | chr1.3:16529322-16529341 | MS.gene62991:CDS | 35.0% | |
| ! | TCTTGTCTTGTTAGAATCTG+AGG | + | chr1.3:16529043-16529062 | MS.gene62991:CDS | 35.0% |
| AAAGAAGAGTTGACGAATGG+CGG | + | chr1.3:16529293-16529312 | MS.gene62991:CDS | 40.0% | |
| AACTCTTTGTGTGCTTCCAA+TGG | - | chr1.3:16529098-16529117 | None:intergenic | 40.0% | |
| AACTTCTCAACATCTGCCTT+TGG | - | chr1.3:16529341-16529360 | None:intergenic | 40.0% | |
| AGGAAAGTCGAGTTACAAGA+AGG | + | chr1.3:16529156-16529175 | MS.gene62991:CDS | 40.0% | |
| AGTTACAAGAAGGGTTCGTT+CGG | + | chr1.3:16529166-16529185 | MS.gene62991:CDS | 40.0% | |
| CTTGTAACTCGACTTTCCTT+GGG | - | chr1.3:16529155-16529174 | None:intergenic | 40.0% | |
| GATTCTAACAAGACAAGACC+AGG | - | chr1.3:16529041-16529060 | None:intergenic | 40.0% | |
| GGAAAAGAAGAGTTGACGAA+TGG | + | chr1.3:16529290-16529309 | MS.gene62991:CDS | 40.0% | |
| GGAAAGTCGAGTTACAAGAA+GGG | + | chr1.3:16529157-16529176 | MS.gene62991:CDS | 40.0% | |
| TCTTGTAACTCGACTTTCCT+TGG | - | chr1.3:16529156-16529175 | None:intergenic | 40.0% | |
| ! | TCTGAGGAAGTGAAGCATTA+TGG | + | chr1.3:16529059-16529078 | MS.gene62991:CDS | 40.0% |
| ! | TTTTCTTGTGGAACTTCCCA+AGG | + | chr1.3:16529136-16529155 | MS.gene62991:CDS | 40.0% |
| AAGAAGAGTTGACGAATGGC+GGG | + | chr1.3:16529294-16529313 | MS.gene62991:CDS | 45.0% | |
| GAAACCAATGAACGACTTCG+AGG | + | chr1.3:16529265-16529284 | MS.gene62991:CDS | 45.0% | |
| GTGATCCTTAAGCACTTCAC+CGG | - | chr1.3:16529018-16529037 | None:intergenic | 45.0% | |
| GTTACAAGAAGGGTTCGTTC+GGG | + | chr1.3:16529167-16529186 | MS.gene62991:CDS | 45.0% | |
| TTACAAGAAGGGTTCGTTCG+GGG | + | chr1.3:16529168-16529187 | MS.gene62991:CDS | 45.0% | |
| ! | AGAGTCTGTCTTTAGCTCGA+AGG | + | chr1.3:16529219-16529238 | MS.gene62991:CDS | 45.0% |
| ACCAATGAACGACTTCGAGG+TGG | + | chr1.3:16529268-16529287 | MS.gene62991:CDS | 50.0% | |
| AGAAGAGTTGACGAATGGCG+GGG | + | chr1.3:16529295-16529314 | MS.gene62991:CDS | 50.0% | |
| ATGCATTGGAAGGGTAGCAG+AGG | + | chr1.3:16529455-16529474 | MS.gene62991:CDS | 50.0% | |
| CCAATGAACGACTTCGAGGT+GGG | + | chr1.3:16529269-16529288 | MS.gene62991:CDS | 50.0% | |
| CTAAAGACACCGGTGAAAGC+CGG | + | chr1.3:16528996-16529015 | MS.gene62991:CDS | 50.0% | |
| ! | GAAAGCCGGTGAAGTGCTTA+AGG | + | chr1.3:16529010-16529029 | MS.gene62991:CDS | 50.0% |
| ! | GAAGTGCTTAAGGATCACCC+TGG | + | chr1.3:16529020-16529039 | MS.gene62991:CDS | 50.0% |
| CCCACCTCGAAGTCGTTCAT+TGG | - | chr1.3:16529272-16529291 | None:intergenic | 55.0% | |
| TGGAAGGGTAGCAGAGGAGT+TGG | + | chr1.3:16529461-16529480 | MS.gene62991:CDS | 55.0% | |
| TGGAGTGAGAGCGAAGCCAT+TGG | + | chr1.3:16529079-16529098 | MS.gene62991:CDS | 55.0% | |
| !! | AGCACTTCACCGGCTTTCAC+CGG | - | chr1.3:16529008-16529027 | None:intergenic | 55.0% |
| ! | AGTTGACGAATGGCGGGGTG+AGG | + | chr1.3:16529300-16529319 | MS.gene62991:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 16528930 | 16529511 | 16528930 | ID=MS.gene62991 |
| chr1.3 | mRNA | 16528930 | 16529511 | 16528930 | ID=MS.gene62991.t1;Parent=MS.gene62991 |
| chr1.3 | exon | 16528930 | 16529511 | 16528930 | ID=MS.gene62991.t1.exon1;Parent=MS.gene62991.t1 |
| chr1.3 | CDS | 16528930 | 16529511 | 16528930 | ID=cds.MS.gene62991.t1;Parent=MS.gene62991.t1 |
| Gene Sequence |
| Protein sequence |
