Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63819.t1 | XP_024640815.1 | 62.6 | 334 | 67 | 8 | 1 | 290 | 1 | 320 | 2.90E-49 | 206.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63819.t1 | G7K4I5 | 58.2 | 359 | 69 | 9 | 1 | 290 | 1 | 347 | 3.5e-44 | 188.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene63819 | 0.82847 | 8.83E-55 | -1.69E-46 |
| MS.gene051626 | MS.gene63819 | 0.81589 | 7.33E-52 | -1.69E-46 |
| MS.gene051662 | MS.gene63819 | 0.808179 | 3.52E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene63819 | 0.811151 | 8.08E-51 | -1.69E-46 |
| MS.gene051789 | MS.gene63819 | 0.801882 | 7.30E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene63819 | 0.805566 | 1.26E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene63819 | 0.829291 | 5.59E-55 | -1.69E-46 |
| MS.gene054184 | MS.gene63819 | 0.811937 | 5.45E-51 | -1.69E-46 |
| MS.gene054185 | MS.gene63819 | 0.818252 | 2.16E-52 | -1.69E-46 |
| MS.gene054473 | MS.gene63819 | 0.8123 | 4.54E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene63819 | 0.801668 | 8.08E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene63819 | 0.818828 | 1.60E-52 | -1.69E-46 |
| MS.gene056069 | MS.gene63819 | -0.828933 | 6.83E-55 | -1.69E-46 |
| MS.gene056875 | MS.gene63819 | 0.802031 | 6.80E-49 | -1.69E-46 |
| MS.gene057260 | MS.gene63819 | 0.823198 | 1.58E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene63819 | 0.805304 | 1.42E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene63819 | 0.810076 | 1.38E-50 | -1.69E-46 |
| MS.gene060476 | MS.gene63819 | 0.841586 | 4.34E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63819.t1 | MTR_5g094950 | 57.103 | 359 | 73 | 8 | 1 | 290 | 1 | 347 | 1.22e-105 | 322 |
| MS.gene63819.t1 | MTR_5g094970 | 51.104 | 317 | 69 | 8 | 70 | 316 | 6 | 306 | 1.97e-75 | 237 |
| MS.gene63819.t1 | MTR_5g095050 | 36.945 | 563 | 160 | 17 | 1 | 372 | 1 | 559 | 1.34e-64 | 215 |
| MS.gene63819.t1 | MTR_5g094960 | 40.854 | 328 | 107 | 9 | 1 | 290 | 1 | 279 | 4.87e-52 | 180 |
| MS.gene63819.t1 | MTR_5g094850 | 40.058 | 342 | 132 | 9 | 3 | 309 | 1 | 304 | 1.33e-49 | 174 |
| MS.gene63819.t1 | MTR_5g094850 | 40.058 | 342 | 132 | 9 | 3 | 309 | 1 | 304 | 2.26e-49 | 174 |
| MS.gene63819.t1 | MTR_5g094920 | 34.596 | 396 | 107 | 9 | 1 | 290 | 1 | 350 | 1.55e-38 | 145 |
| MS.gene63819.t1 | MTR_5g094990 | 40.705 | 312 | 124 | 13 | 1 | 290 | 1 | 273 | 1.52e-36 | 138 |
| MS.gene63819.t1 | MTR_5g095370 | 41.206 | 199 | 70 | 5 | 1 | 196 | 1 | 155 | 8.24e-32 | 120 |
| MS.gene63819.t1 | MTR_5g094910 | 34.731 | 334 | 104 | 11 | 1 | 301 | 1 | 253 | 3.04e-28 | 114 |
| MS.gene63819.t1 | MTR_5g095400 | 46.341 | 164 | 56 | 3 | 26 | 185 | 1 | 136 | 1.10e-27 | 114 |
| MS.gene63819.t1 | MTR_5g095430 | 45.806 | 155 | 52 | 3 | 35 | 185 | 1 | 127 | 9.89e-27 | 111 |
| MS.gene63819.t1 | MTR_5g077500 | 45.122 | 164 | 59 | 4 | 178 | 311 | 97 | 259 | 1.67e-24 | 102 |
| MS.gene63819.t1 | MTR_5g095390 | 44.086 | 186 | 67 | 5 | 30 | 202 | 20 | 181 | 2.64e-21 | 94.4 |
| MS.gene63819.t1 | MTR_5g095310 | 39.456 | 147 | 61 | 3 | 35 | 179 | 1 | 121 | 1.66e-19 | 89.4 |
| MS.gene63819.t1 | MTR_5g095360 | 41.121 | 107 | 52 | 3 | 61 | 158 | 1 | 105 | 1.61e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGACCCATGTTCGATTT+TGG | 0.133002 | 5.3:-75977101 | None:intergenic |
| CTCAAAACCATTACATGTTA+AGG | 0.367230 | 5.3:+75978116 | MS.gene63819:CDS |
| TGAGTTAGATAATGAAGTTT+TGG | 0.377487 | 5.3:+75977660 | MS.gene63819:CDS |
| GTCATTTCTTCTAAGTTATG+AGG | 0.381921 | 5.3:-75977172 | None:intergenic |
| CTTACAACACAACGTTGAAA+TGG | 0.390778 | 5.3:+75977306 | MS.gene63819:CDS |
| TTGACATCCTTAACATGTAA+TGG | 0.392334 | 5.3:-75978123 | None:intergenic |
| GGGCTGCTTGCATCTTTGCC+TGG | 0.393057 | 5.3:-75978207 | None:intergenic |
| GAGAGGAACTTTGTCAGAAA+GGG | 0.410933 | 5.3:+75977371 | MS.gene63819:CDS |
| TTAGCTGATAGAGTCACTAT+TGG | 0.415973 | 5.3:+75977529 | MS.gene63819:CDS |
| TGAGCTAGAAGCTAAAGTTT+TGG | 0.417553 | 5.3:+75977747 | MS.gene63819:CDS |
| CCAATCTTCGGGTCACTCTC+TGG | 0.419534 | 5.3:-75978277 | None:intergenic |
| AAGAAAGAAGTACGTTGAGT+TGG | 0.438659 | 5.3:+75977558 | MS.gene63819:CDS |
| GGAGGATGTTGAAGCAAGTC+AGG | 0.455376 | 5.3:+75977951 | MS.gene63819:CDS |
| CGAGCTTCGAAGTAAATTGA+AGG | 0.461338 | 5.3:+75977819 | MS.gene63819:CDS |
| GACACATGAGGTGTTGCAAC+CGG | 0.466770 | 5.3:-75977994 | None:intergenic |
| AGAGAGGAACTTTGTCAGAA+AGG | 0.484616 | 5.3:+75977370 | MS.gene63819:CDS |
| ATGTCAAGAAAGCCAAAGTC+AGG | 0.490710 | 5.3:+75978139 | MS.gene63819:CDS |
| GAAGATGGTGATGAGGTTGA+TGG | 0.497731 | 5.3:+75978240 | MS.gene63819:CDS |
| TCTAACACTAGACCTGACTT+TGG | 0.506997 | 5.3:-75978151 | None:intergenic |
| AGCAAGAATCACGTAGTTGG+TGG | 0.527719 | 5.3:-75977125 | None:intergenic |
| AACAAGATGATTGATGGTCC+AGG | 0.535173 | 5.3:+75978189 | MS.gene63819:CDS |
| GAGGTTGATGGTATTGATGA+TGG | 0.540674 | 5.3:+75978252 | MS.gene63819:CDS |
| CTTATTCGAGTCTTTGAACA+CGG | 0.544179 | 5.3:+75977894 | MS.gene63819:CDS |
| TTCAGCAAGAATCACGTAGT+TGG | 0.558811 | 5.3:-75977128 | None:intergenic |
| GCATGAAAGAAATGCAAGGT+TGG | 0.577609 | 5.3:+75977930 | MS.gene63819:CDS |
| TGAGTTGAGGAAACTGTTTG+AGG | 0.587379 | 5.3:+75977489 | MS.gene63819:CDS |
| TGAAGTTGTAACAGATATGA+TGG | 0.587982 | 5.3:+75977147 | MS.gene63819:CDS |
| TCACCATCTTCAATCACAAG+TGG | 0.589505 | 5.3:-75978228 | None:intergenic |
| AGCCCACTTGTGATTGAAGA+TGG | 0.597478 | 5.3:+75978225 | MS.gene63819:CDS |
| GAAAGAGTTTCAGAAAAGAG+AGG | 0.598100 | 5.3:+75977354 | MS.gene63819:CDS |
| TACTATAAATGAGAAGCGTA+AGG | 0.599833 | 5.3:+75977864 | MS.gene63819:CDS |
| ATTGAACCCAAAATCGAACA+TGG | 0.604289 | 5.3:+75977094 | MS.gene63819:CDS |
| GTACGTTGAGTTGGAGTGTG+AGG | 0.619740 | 5.3:+75977567 | MS.gene63819:CDS |
| TGATCATGTTGCAAATGCAC+CGG | 0.620646 | 5.3:+75977975 | MS.gene63819:CDS |
| GCAACACCTCATGTGTCTGA+TGG | 0.630963 | 5.3:+75978000 | MS.gene63819:CDS |
| GAGAGAGGAACATTGAGTTG+AGG | 0.631931 | 5.3:+75977476 | MS.gene63819:CDS |
| TGAAAGAAATGCAAGGTTGG+AGG | 0.632876 | 5.3:+75977933 | MS.gene63819:CDS |
| TCTTTGAACACGGAGTTCAG+AGG | 0.634013 | 5.3:+75977904 | MS.gene63819:CDS |
| TTGAAATGGAGAAACTTGCA+AGG | 0.634595 | 5.3:+75977320 | MS.gene63819:CDS |
| CACCATCTTCAATCACAAGT+GGG | 0.636322 | 5.3:-75978227 | None:intergenic |
| CTGTTGTAGTACTAAGAGAG+AGG | 0.656292 | 5.3:+75977461 | MS.gene63819:CDS |
| TGTGATTGAAGATGGTGATG+AGG | 0.677350 | 5.3:+75978233 | MS.gene63819:CDS |
| GATTGCATGAAAGAAATGCA+AGG | 0.680697 | 5.3:+75977926 | MS.gene63819:CDS |
| TTGAACCCAAAATCGAACAT+GGG | 0.683973 | 5.3:+75977095 | MS.gene63819:CDS |
| TTTGTACCATCAGACACATG+AGG | 0.707522 | 5.3:-75978006 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTTTAAGTTATATTTTAC+AGG | + | chr5.3:75978080-75978099 | MS.gene63819:intron | 15.0% |
| ! | TTGAAAAACAAGATGATTGA+TGG | + | chr5.3:75978183-75978202 | MS.gene63819:CDS | 25.0% |
| !! | GAAGTACTAAGAAAAAAGAA+TGG | + | chr5.3:75977637-75977656 | MS.gene63819:CDS | 25.0% |
| !! | TGAGTTAGATAATGAAGTTT+TGG | + | chr5.3:75977660-75977679 | MS.gene63819:CDS | 25.0% |
| CTCAAAACCATTACATGTTA+AGG | + | chr5.3:75978116-75978135 | MS.gene63819:CDS | 30.0% | |
| GAAGTTGAAAGAAAAATGTG+TGG | + | chr5.3:75977594-75977613 | MS.gene63819:CDS | 30.0% | |
| GTCATTTCTTCTAAGTTATG+AGG | - | chr5.3:75977175-75977194 | None:intergenic | 30.0% | |
| TGAAGTTGTAACAGATATGA+TGG | + | chr5.3:75977147-75977166 | MS.gene63819:CDS | 30.0% | |
| TTGACATCCTTAACATGTAA+TGG | - | chr5.3:75978126-75978145 | None:intergenic | 30.0% | |
| ! | TACTATAAATGAGAAGCGTA+AGG | + | chr5.3:75977864-75977883 | MS.gene63819:CDS | 30.0% |
| ! | TGGAGATTTTGAATGAGAAA+TGG | + | chr5.3:75977773-75977792 | MS.gene63819:CDS | 30.0% |
| ! | TTTGGAGTTGAAGCAAAAAA+GGG | + | chr5.3:75977678-75977697 | MS.gene63819:CDS | 30.0% |
| !!! | TTTTGGAGTTGAAGCAAAAA+AGG | + | chr5.3:75977677-75977696 | MS.gene63819:CDS | 30.0% |
| AAAAATGTGTGGAGGATGAT+AGG | + | chr5.3:75977605-75977624 | MS.gene63819:CDS | 35.0% | |
| ATTGAACCCAAAATCGAACA+TGG | + | chr5.3:75977094-75977113 | MS.gene63819:CDS | 35.0% | |
| CTTACAACACAACGTTGAAA+TGG | + | chr5.3:75977306-75977325 | MS.gene63819:CDS | 35.0% | |
| CTTATTCGAGTCTTTGAACA+CGG | + | chr5.3:75977894-75977913 | MS.gene63819:CDS | 35.0% | |
| GAAAGAGTTTCAGAAAAGAG+AGG | + | chr5.3:75977354-75977373 | MS.gene63819:CDS | 35.0% | |
| GATTGCATGAAAGAAATGCA+AGG | + | chr5.3:75977926-75977945 | MS.gene63819:CDS | 35.0% | |
| GTTGAAAGAAAAATGTGTGG+AGG | + | chr5.3:75977597-75977616 | MS.gene63819:CDS | 35.0% | |
| TTAGCTGATAGAGTCACTAT+TGG | + | chr5.3:75977529-75977548 | MS.gene63819:CDS | 35.0% | |
| TTGAAATGGAGAAACTTGCA+AGG | + | chr5.3:75977320-75977339 | MS.gene63819:CDS | 35.0% | |
| TTGAACCCAAAATCGAACAT+GGG | + | chr5.3:75977095-75977114 | MS.gene63819:CDS | 35.0% | |
| ! | AAGAAAGAAGTACGTTGAGT+TGG | + | chr5.3:75977558-75977577 | MS.gene63819:CDS | 35.0% |
| ! | TGAGCTAGAAGCTAAAGTTT+TGG | + | chr5.3:75977747-75977766 | MS.gene63819:CDS | 35.0% |
| !!! | AGAAGCTAAAGTTTTGGAGT+TGG | + | chr5.3:75977753-75977772 | MS.gene63819:CDS | 35.0% |
| AGAGAGGAACTTTGTCAGAA+AGG | + | chr5.3:75977370-75977389 | MS.gene63819:CDS | 40.0% | |
| ATGTCAAGAAAGCCAAAGTC+AGG | + | chr5.3:75978139-75978158 | MS.gene63819:CDS | 40.0% | |
| CACCATCTTCAATCACAAGT+GGG | - | chr5.3:75978230-75978249 | None:intergenic | 40.0% | |
| CGAGCTTCGAAGTAAATTGA+AGG | + | chr5.3:75977819-75977838 | MS.gene63819:CDS | 40.0% | |
| CTGTTGTAGTACTAAGAGAG+AGG | + | chr5.3:75977461-75977480 | MS.gene63819:CDS | 40.0% | |
| GAGAGGAACTTTGTCAGAAA+GGG | + | chr5.3:75977371-75977390 | MS.gene63819:CDS | 40.0% | |
| GCATGAAAGAAATGCAAGGT+TGG | + | chr5.3:75977930-75977949 | MS.gene63819:CDS | 40.0% | |
| TCACCATCTTCAATCACAAG+TGG | - | chr5.3:75978231-75978250 | None:intergenic | 40.0% | |
| TCTAACACTAGACCTGACTT+TGG | - | chr5.3:75978154-75978173 | None:intergenic | 40.0% | |
| TGAAAGAAATGCAAGGTTGG+AGG | + | chr5.3:75977933-75977952 | MS.gene63819:CDS | 40.0% | |
| TGATCATGTTGCAAATGCAC+CGG | + | chr5.3:75977975-75977994 | MS.gene63819:CDS | 40.0% | |
| TTCAGCAAGAATCACGTAGT+TGG | - | chr5.3:75977131-75977150 | None:intergenic | 40.0% | |
| TTTGTACCATCAGACACATG+AGG | - | chr5.3:75978009-75978028 | None:intergenic | 40.0% | |
| ! | AACAAGATGATTGATGGTCC+AGG | + | chr5.3:75978189-75978208 | MS.gene63819:CDS | 40.0% |
| ! | AGATGACCCATGTTCGATTT+TGG | - | chr5.3:75977104-75977123 | None:intergenic | 40.0% |
| ! | GATGACCCATGTTCGATTTT+GGG | - | chr5.3:75977103-75977122 | None:intergenic | 40.0% |
| ! | TGAGTTGAGGAAACTGTTTG+AGG | + | chr5.3:75977489-75977508 | MS.gene63819:CDS | 40.0% |
| ! | TGTGATTGAAGATGGTGATG+AGG | + | chr5.3:75978233-75978252 | MS.gene63819:CDS | 40.0% |
| !! | GAGGTTGATGGTATTGATGA+TGG | + | chr5.3:75978252-75978271 | MS.gene63819:CDS | 40.0% |
| AAGCAAAAAAGGGTGGAGGA+TGG | + | chr5.3:75977688-75977707 | MS.gene63819:CDS | 45.0% | |
| AGCAAAAAAGGGTGGAGGAT+GGG | + | chr5.3:75977689-75977708 | MS.gene63819:CDS | 45.0% | |
| AGCAAGAATCACGTAGTTGG+TGG | - | chr5.3:75977128-75977147 | None:intergenic | 45.0% | |
| AGCCCACTTGTGATTGAAGA+TGG | + | chr5.3:75978225-75978244 | MS.gene63819:CDS | 45.0% | |
| GGAGTTGAAGCAAAAAAGGG+TGG | + | chr5.3:75977681-75977700 | MS.gene63819:CDS | 45.0% | |
| GTTGAAGCAAAAAAGGGTGG+AGG | + | chr5.3:75977684-75977703 | MS.gene63819:CDS | 45.0% | |
| TCTTTGAACACGGAGTTCAG+AGG | + | chr5.3:75977904-75977923 | MS.gene63819:CDS | 45.0% | |
| ! | GAAGATGGTGATGAGGTTGA+TGG | + | chr5.3:75978240-75978259 | MS.gene63819:CDS | 45.0% |
| ! | GAGAGAGGAACATTGAGTTG+AGG | + | chr5.3:75977476-75977495 | MS.gene63819:CDS | 45.0% |
| GCAACACCTCATGTGTCTGA+TGG | + | chr5.3:75978000-75978019 | MS.gene63819:CDS | 50.0% | |
| GGAGGATGTTGAAGCAAGTC+AGG | + | chr5.3:75977951-75977970 | MS.gene63819:CDS | 50.0% | |
| !! | GACACATGAGGTGTTGCAAC+CGG | - | chr5.3:75977997-75978016 | None:intergenic | 50.0% |
| !! | GTACGTTGAGTTGGAGTGTG+AGG | + | chr5.3:75977567-75977586 | MS.gene63819:CDS | 50.0% |
| GGGCTGCTTGCATCTTTGCC+TGG | - | chr5.3:75978210-75978229 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 75977088 | 75978287 | 75977088 | ID=MS.gene63819 |
| chr5.3 | mRNA | 75977088 | 75978287 | 75977088 | ID=MS.gene63819.t1;Parent=MS.gene63819 |
| chr5.3 | exon | 75977088 | 75978029 | 75977088 | ID=MS.gene63819.t1.exon1;Parent=MS.gene63819.t1 |
| chr5.3 | CDS | 75977088 | 75978029 | 75977088 | ID=cds.MS.gene63819.t1;Parent=MS.gene63819.t1 |
| chr5.3 | exon | 75978102 | 75978287 | 75978102 | ID=MS.gene63819.t1.exon2;Parent=MS.gene63819.t1 |
| chr5.3 | CDS | 75978102 | 75978287 | 75978102 | ID=cds.MS.gene63819.t1;Parent=MS.gene63819.t1 |
| Gene Sequence |
| Protein sequence |
