Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64235.t1 | XP_003596287.1 | 70.1 | 127 | 27 | 1 | 1 | 127 | 1 | 116 | 1.10E-22 | 116.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64235.t1 | G7IR17 | 70.1 | 127 | 27 | 1 | 1 | 127 | 1 | 116 | 7.9e-23 | 116.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050129 | MS.gene64235 | 0.803617 | 3.20E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene64235 | 0.804533 | 2.06E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene64235 | 0.819454 | 1.15E-52 | -1.69E-46 |
| MS.gene050883 | MS.gene64235 | 0.838956 | 2.11E-57 | -1.69E-46 |
| MS.gene05141 | MS.gene64235 | 0.80881 | 2.58E-50 | -1.69E-46 |
| MS.gene051946 | MS.gene64235 | 0.827698 | 1.35E-54 | -1.69E-46 |
| MS.gene053549 | MS.gene64235 | 0.822109 | 2.82E-53 | -1.69E-46 |
| MS.gene054708 | MS.gene64235 | 0.809269 | 2.06E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene64235 | 0.851803 | 7.00E-61 | -1.69E-46 |
| MS.gene056311 | MS.gene64235 | 0.849851 | 2.48E-60 | -1.69E-46 |
| MS.gene056313 | MS.gene64235 | 0.830544 | 2.77E-55 | -1.69E-46 |
| MS.gene057014 | MS.gene64235 | 0.843163 | 1.66E-58 | -1.69E-46 |
| MS.gene06077 | MS.gene64235 | -0.815892 | 7.32E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64235.t1 | MTR_2g075540 | 63.704 | 135 | 30 | 2 | 1 | 131 | 1 | 120 | 1.20e-36 | 122 |
| MS.gene64235.t1 | MTR_2g075600 | 47.682 | 151 | 57 | 2 | 1 | 131 | 1 | 149 | 4.70e-32 | 111 |
| MS.gene64235.t1 | MTR_2g075590 | 57.037 | 135 | 37 | 3 | 1 | 131 | 1 | 118 | 5.38e-31 | 107 |
| MS.gene64235.t1 | MTR_2g075550 | 51.408 | 142 | 49 | 4 | 1 | 124 | 1 | 140 | 5.42e-30 | 105 |
| MS.gene64235.t1 | MTR_2g075570 | 56.557 | 122 | 40 | 3 | 1 | 113 | 1 | 118 | 2.04e-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTAAAGCACATTAATT+AGG | 0.248204 | 2.1:+24039568 | MS.gene64235:CDS |
| AGCACAGGAAGCGGAGGATT+GGG | 0.335111 | 2.1:+24039295 | MS.gene64235:CDS |
| GGTGGATTCTTTCGATTACT+TGG | 0.383958 | 2.1:-24039385 | None:intergenic |
| TAGCACAGGAAGCGGAGGAT+TGG | 0.389817 | 2.1:+24039294 | MS.gene64235:CDS |
| AGCACAGGCGGAGGAGGATT+GGG | 0.392310 | 2.1:+24039412 | MS.gene64235:CDS |
| TAGCACAGGCGGAGGAGGAT+TGG | 0.413090 | 2.1:+24039411 | MS.gene64235:CDS |
| CTCCGCTTCCTGTGCTACCC+GGG | 0.415668 | 2.1:-24039288 | None:intergenic |
| CCTCCTCCGCCTGTGCTACC+TGG | 0.415974 | 2.1:-24039406 | None:intergenic |
| CCTCCGCTTCCTGTGCTACC+CGG | 0.427212 | 2.1:-24039289 | None:intergenic |
| GGCGGAGGAGGATTGGGAAC+TGG | 0.445749 | 2.1:+24039418 | MS.gene64235:CDS |
| GGGTTGGCAGAGCTGGTATG+TGG | 0.457229 | 2.1:-24038810 | None:intergenic |
| TTTCAAATGGCTCTTGCTGC+AGG | 0.461051 | 2.1:+24038778 | None:intergenic |
| CCAGGTAGCACAGGCGGAGG+AGG | 0.487226 | 2.1:+24039406 | MS.gene64235:CDS |
| GGTTGGCAGAGCTGGTATGT+GGG | 0.497814 | 2.1:-24038809 | None:intergenic |
| TCCGCTTCCTGTGCTACCCG+GGG | 0.514938 | 2.1:-24039287 | None:intergenic |
| ATTGGGGACTGGTCCGAAAA+CGG | 0.529672 | 2.1:+24039312 | MS.gene64235:CDS |
| GCACAGGAAGCGGAGGATTG+GGG | 0.533668 | 2.1:+24039296 | MS.gene64235:CDS |
| TGCACAAACAACTAGTACTC+AGG | 0.551675 | 2.1:+24039441 | MS.gene64235:CDS |
| GGAAGCGGAGGATTGGGGAC+TGG | 0.557972 | 2.1:+24039301 | MS.gene64235:CDS |
| AAGAATCCACCAGGTAGCAC+AGG | 0.570025 | 2.1:+24039397 | MS.gene64235:CDS |
| TCAATCTTACTGCCAATGAG+TGG | 0.586404 | 2.1:+24039503 | MS.gene64235:CDS |
| AACAACTAGTACTCAGGTGG+AGG | 0.594211 | 2.1:+24039447 | MS.gene64235:CDS |
| CCTCCGCCTGTGCTACCTGG+TGG | 0.595864 | 2.1:-24039403 | None:intergenic |
| CCACCAGGTAGCACAGGCGG+AGG | 0.597247 | 2.1:+24039403 | MS.gene64235:CDS |
| CCGGGTAGCACAGGAAGCGG+AGG | 0.602411 | 2.1:+24039289 | MS.gene64235:CDS |
| AATTGATCGAAAGAAACCCC+CGG | 0.603280 | 2.1:+24039270 | MS.gene64235:CDS |
| CAATCTTACTGCCAATGAGT+GGG | 0.616463 | 2.1:+24039504 | MS.gene64235:CDS |
| GAGAATACTCAGCGACGTGG+AGG | 0.622518 | 2.1:+24039334 | MS.gene64235:CDS |
| AATCCACCAGGTAGCACAGG+CGG | 0.634430 | 2.1:+24039400 | MS.gene64235:CDS |
| AGTAATCGAAAGAATCCACC+AGG | 0.647364 | 2.1:+24039388 | MS.gene64235:CDS |
| CCGCTTCCTGTGCTACCCGG+GGG | 0.651421 | 2.1:-24039286 | None:intergenic |
| TCTTACTGCCAATGAGTGGG+TGG | 0.702286 | 2.1:+24039507 | MS.gene64235:CDS |
| ACGGAGAATACTCAGCGACG+TGG | 0.705185 | 2.1:+24039331 | MS.gene64235:CDS |
| ACAAACAACTAGTACTCAGG+TGG | 0.706249 | 2.1:+24039444 | MS.gene64235:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTTATAATAAACTATCAT+TGG | + | chr2.1:24039165-24039184 | MS.gene64235:intron | 15.0% |
| !!! | AATAAATTTTTAACTTGTTC+AGG | - | chr2.1:24039069-24039088 | None:intergenic | 15.0% |
| !!! | AGATTTTATAAATCAGTATA+GGG | + | chr2.1:24039124-24039143 | MS.gene64235:intron | 15.0% |
| !!! | ATAAATTTTTAACTTGTTCA+GGG | - | chr2.1:24039068-24039087 | None:intergenic | 15.0% |
| !!! | TACTGATTTATAAAATCTAT+AGG | - | chr2.1:24039122-24039141 | None:intergenic | 15.0% |
| !!! | TAGATTTTATAAATCAGTAT+AGG | + | chr2.1:24039123-24039142 | MS.gene64235:intron | 15.0% |
| !! | TAATTATATACGTATGCTTA+AGG | + | chr2.1:24038903-24038922 | MS.gene64235:intron | 20.0% |
| !!! | GTAATTGTCATAGATTTTTT+AGG | - | chr2.1:24038847-24038866 | None:intergenic | 20.0% |
| ! | TTTAAATTAGCCATGATTCA+TGG | + | chr2.1:24039019-24039038 | MS.gene64235:intron | 25.0% |
| !!! | AGTATAGGGAATTTTTTGTA+TGG | + | chr2.1:24039138-24039157 | MS.gene64235:intron | 25.0% |
| !!! | TGTTTTGTTTTTCTTGTTGT+TGG | - | chr2.1:24039248-24039267 | None:intergenic | 25.0% |
| AAAACCACCAAAAAATTCTG+AGG | + | chr2.1:24039360-24039379 | MS.gene64235:CDS | 30.0% | |
| GTTAATCAAACCATGAATCA+TGG | - | chr2.1:24039032-24039051 | None:intergenic | 30.0% | |
| !!! | AGATTTTTTAGGCACATAGT+TGG | - | chr2.1:24038836-24038855 | None:intergenic | 30.0% |
| !!! | ATTTTTTAGGCACATAGTTG+GGG | - | chr2.1:24038834-24038853 | None:intergenic | 30.0% |
| !!! | GATTTTTTAGGCACATAGTT+GGG | - | chr2.1:24038835-24038854 | None:intergenic | 30.0% |
| !!! | CTTTTTTTGACCCTCTTTGT+AGG | + | chr2.1:24039538-24039557 | MS.gene64235:intron | 35.0% |
| !!! | TTTTTTAGGCACATAGTTGG+GGG | - | chr2.1:24038833-24038852 | None:intergenic | 35.0% |
| AAAAATCACCACCCACTCAT+TGG | - | chr2.1:24039518-24039537 | None:intergenic | 40.0% | |
| AATTGATCGAAAGAAACCCC+CGG | + | chr2.1:24039270-24039289 | MS.gene64235:CDS | 40.0% | |
| ACAAACAACTAGTACTCAGG+TGG | + | chr2.1:24039444-24039463 | MS.gene64235:CDS | 40.0% | |
| ACAATGTCGAACCTACAAAG+AGG | - | chr2.1:24039552-24039571 | None:intergenic | 40.0% | |
| AGTAATCGAAAGAATCCACC+AGG | + | chr2.1:24039388-24039407 | MS.gene64235:CDS | 40.0% | |
| CAATCTTACTGCCAATGAGT+GGG | + | chr2.1:24039504-24039523 | MS.gene64235:CDS | 40.0% | |
| CAATGTCGAACCTACAAAGA+GGG | - | chr2.1:24039551-24039570 | None:intergenic | 40.0% | |
| TCAATCTTACTGCCAATGAG+TGG | + | chr2.1:24039503-24039522 | MS.gene64235:CDS | 40.0% | |
| TGCACAAACAACTAGTACTC+AGG | + | chr2.1:24039441-24039460 | MS.gene64235:CDS | 40.0% | |
| ! | GGTGGATTCTTTCGATTACT+TGG | - | chr2.1:24039388-24039407 | None:intergenic | 40.0% |
| !!! | CTTGGTGCCTCAGAATTTTT+TGG | - | chr2.1:24039370-24039389 | None:intergenic | 40.0% |
| AACAACTAGTACTCAGGTGG+AGG | + | chr2.1:24039447-24039466 | MS.gene64235:CDS | 45.0% | |
| ATTGATCGAAAGAAACCCCC+GGG | + | chr2.1:24039271-24039290 | MS.gene64235:CDS | 45.0% | |
| ! | CGCTGAGTATTCTCCGTTTT+CGG | - | chr2.1:24039328-24039347 | None:intergenic | 45.0% |
| !!! | GGTGCCTCAGAATTTTTTGG+TGG | - | chr2.1:24039367-24039386 | None:intergenic | 45.0% |
| AAGAATCCACCAGGTAGCAC+AGG | + | chr2.1:24039397-24039416 | MS.gene64235:CDS | 50.0% | |
| TCTTACTGCCAATGAGTGGG+TGG | + | chr2.1:24039507-24039526 | MS.gene64235:CDS | 50.0% | |
| TTAGGCACATAGTTGGGGGT+TGG | - | chr2.1:24038829-24038848 | None:intergenic | 50.0% | |
| !! | ATTGGGGACTGGTCCGAAAA+CGG | + | chr2.1:24039312-24039331 | MS.gene64235:CDS | 50.0% |
| AATCCACCAGGTAGCACAGG+CGG | + | chr2.1:24039400-24039419 | MS.gene64235:CDS | 55.0% | |
| ACGGAGAATACTCAGCGACG+TGG | + | chr2.1:24039331-24039350 | MS.gene64235:CDS | 55.0% | |
| AGCACAGGAAGCGGAGGATT+GGG | + | chr2.1:24039295-24039314 | MS.gene64235:CDS | 55.0% | |
| GAGAATACTCAGCGACGTGG+AGG | + | chr2.1:24039334-24039353 | MS.gene64235:CDS | 55.0% | |
| GGTTGGCAGAGCTGGTATGT+GGG | - | chr2.1:24038812-24038831 | None:intergenic | 55.0% | |
| TAGCACAGGAAGCGGAGGAT+TGG | + | chr2.1:24039294-24039313 | MS.gene64235:CDS | 55.0% | |
| AAGAAACCCCCGGGTAGCAC+AGG | + | chr2.1:24039280-24039299 | MS.gene64235:CDS | 60.0% | |
| AGCACAGGCGGAGGAGGATT+GGG | + | chr2.1:24039412-24039431 | MS.gene64235:CDS | 60.0% | |
| GCACAGGAAGCGGAGGATTG+GGG | + | chr2.1:24039296-24039315 | MS.gene64235:CDS | 60.0% | |
| TAGCACAGGCGGAGGAGGAT+TGG | + | chr2.1:24039411-24039430 | MS.gene64235:CDS | 60.0% | |
| ! | GGGTTGGCAGAGCTGGTATG+TGG | - | chr2.1:24038813-24038832 | None:intergenic | 60.0% |
| ! | TAGTTGGGGGTTGGCAGAGC+TGG | - | chr2.1:24038820-24038839 | None:intergenic | 60.0% |
| CCTCCGCTTCCTGTGCTACC+CGG | - | chr2.1:24039292-24039311 | None:intergenic | 65.0% | |
| CTCCGCTTCCTGTGCTACCC+GGG | - | chr2.1:24039291-24039310 | None:intergenic | 65.0% | |
| GGAAGCGGAGGATTGGGGAC+TGG | + | chr2.1:24039301-24039320 | MS.gene64235:CDS | 65.0% | |
| GGCGGAGGAGGATTGGGAAC+TGG | + | chr2.1:24039418-24039437 | MS.gene64235:CDS | 65.0% | |
| TCCGCTTCCTGTGCTACCCG+GGG | - | chr2.1:24039290-24039309 | None:intergenic | 65.0% | |
| CCACCAGGTAGCACAGGCGG+AGG | + | chr2.1:24039403-24039422 | MS.gene64235:CDS | 70.0% | |
| CCAGGTAGCACAGGCGGAGG+AGG | + | chr2.1:24039406-24039425 | MS.gene64235:CDS | 70.0% | |
| CCCCCGGGTAGCACAGGAAG+CGG | + | chr2.1:24039286-24039305 | MS.gene64235:CDS | 70.0% | |
| CCGCTTCCTGTGCTACCCGG+GGG | - | chr2.1:24039289-24039308 | None:intergenic | 70.0% | |
| CCGGGTAGCACAGGAAGCGG+AGG | + | chr2.1:24039289-24039308 | MS.gene64235:CDS | 70.0% | |
| CCTCCGCCTGTGCTACCTGG+TGG | - | chr2.1:24039406-24039425 | None:intergenic | 70.0% | |
| CCTCCTCCGCCTGTGCTACC+TGG | - | chr2.1:24039409-24039428 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 24038784 | 24039589 | 24038784 | ID=MS.gene64235 |
| chr2.1 | mRNA | 24038784 | 24039589 | 24038784 | ID=MS.gene64235.t1;Parent=MS.gene64235 |
| chr2.1 | exon | 24038784 | 24038869 | 24038784 | ID=MS.gene64235.t1.exon1;Parent=MS.gene64235.t1 |
| chr2.1 | CDS | 24038784 | 24038869 | 24038784 | ID=cds.MS.gene64235.t1;Parent=MS.gene64235.t1 |
| chr2.1 | exon | 24039237 | 24039528 | 24039237 | ID=MS.gene64235.t1.exon2;Parent=MS.gene64235.t1 |
| chr2.1 | CDS | 24039237 | 24039528 | 24039237 | ID=cds.MS.gene64235.t1;Parent=MS.gene64235.t1 |
| chr2.1 | exon | 24039560 | 24039589 | 24039560 | ID=MS.gene64235.t1.exon3;Parent=MS.gene64235.t1 |
| chr2.1 | CDS | 24039560 | 24039589 | 24039560 | ID=cds.MS.gene64235.t1;Parent=MS.gene64235.t1 |
| Gene Sequence |
| Protein sequence |
