Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64721.t1 | XP_003612097.2 | 97.2 | 608 | 14 | 2 | 1 | 606 | 1 | 607 | 0.00E+00 | 1170.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64721.t1 | Q8H0V1 | 69.3 | 635 | 159 | 10 | 3 | 605 | 7 | 637 | 1.4e-248 | 860.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64721.t1 | G7KGB3 | 97.2 | 608 | 14 | 2 | 1 | 606 | 1 | 607 | 0.0e+00 | 1170.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene64721 | 0.819254 | 1.28E-52 | -1.69E-46 |
| MS.gene050165 | MS.gene64721 | 0.80709 | 5.99E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene64721 | 0.859272 | 4.64E-63 | -1.69E-46 |
| MS.gene050518 | MS.gene64721 | 0.80289 | 4.53E-49 | -1.69E-46 |
| MS.gene055634 | MS.gene64721 | 0.847811 | 9.13E-60 | -1.69E-46 |
| MS.gene055636 | MS.gene64721 | 0.859487 | 4.00E-63 | -1.69E-46 |
| MS.gene055638 | MS.gene64721 | 0.854019 | 1.63E-61 | -1.69E-46 |
| MS.gene055647 | MS.gene64721 | 0.808088 | 3.68E-50 | -1.69E-46 |
| MS.gene056549 | MS.gene64721 | 0.821404 | 4.11E-53 | -1.69E-46 |
| MS.gene056910 | MS.gene64721 | 0.800238 | 1.58E-48 | -1.69E-46 |
| MS.gene058773 | MS.gene64721 | 0.806493 | 8.01E-50 | -1.69E-46 |
| MS.gene059029 | MS.gene64721 | 0.835476 | 1.64E-56 | -1.69E-46 |
| MS.gene059079 | MS.gene64721 | 0.815568 | 8.65E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene64721 | 0.807585 | 4.70E-50 | -1.69E-46 |
| MS.gene060818 | MS.gene64721 | 0.801548 | 8.55E-49 | -1.69E-46 |
| MS.gene061134 | MS.gene64721 | 0.814572 | 1.44E-51 | -1.69E-46 |
| MS.gene061171 | MS.gene64721 | 0.835024 | 2.13E-56 | -1.69E-46 |
| MS.gene061200 | MS.gene64721 | 0.805717 | 1.17E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64721.t1 | MTR_5g021230 | 97.204 | 608 | 14 | 2 | 1 | 606 | 1 | 607 | 0.0 | 1223 |
| MS.gene64721.t1 | MTR_4g033210 | 24.254 | 503 | 293 | 15 | 95 | 585 | 49 | 475 | 7.33e-33 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64721.t1 | AT4G36390 | 75.719 | 556 | 119 | 5 | 51 | 594 | 75 | 626 | 0.0 | 890 |
| MS.gene64721.t1 | AT1G72090 | 24.800 | 500 | 294 | 14 | 95 | 585 | 61 | 487 | 6.66e-36 | 143 |
Find 131 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTACAGGGGTTGTTGAAAT+TGG | 0.119875 | 5.2:+12420610 | None:intergenic |
| GAAGAGTGTTAATCCCTTTC+TGG | 0.178406 | 5.2:+12419348 | None:intergenic |
| CATTTCTGCATTATCTCTTA+TGG | 0.218289 | 5.2:+12419598 | None:intergenic |
| AGTTCATCTGGATAATCTTT+AGG | 0.241902 | 5.2:+12418918 | None:intergenic |
| CATTGTAGCTGTTCACATTC+TGG | 0.245538 | 5.2:+12419108 | None:intergenic |
| TAGGGATTTGCCACGATTAC+TGG | 0.271983 | 5.2:-12419387 | MS.gene64721:CDS |
| TCTCCAGATACTGAATTAAT+TGG | 0.276590 | 5.2:-12414119 | MS.gene64721:CDS |
| GGCAGAGGCTTAATTACTTT+TGG | 0.281806 | 5.2:-12419568 | MS.gene64721:CDS |
| TTGCCAATTAATTCAGTATC+TGG | 0.282675 | 5.2:+12414116 | None:intergenic |
| TTTGGTTTCTTAAAAGGAAT+TGG | 0.310610 | 5.2:-12419550 | MS.gene64721:CDS |
| GTTCATCTGGATAATCTTTA+GGG | 0.312079 | 5.2:+12418919 | None:intergenic |
| TCAAATGAACGTTAATGATA+TGG | 0.321207 | 5.2:-12419711 | MS.gene64721:CDS |
| GATACTCGATTCAGACAAAA+TGG | 0.355549 | 5.2:-12419438 | MS.gene64721:CDS |
| TGAGAGAGGTGGGAGAGCTT+TGG | 0.363302 | 5.2:-12419163 | MS.gene64721:CDS |
| TCCTCCGAAAGTAGTTGTTT+TGG | 0.364250 | 5.2:-12419489 | MS.gene64721:CDS |
| ATGTATGGCTGAGAGGTTAA+AGG | 0.367483 | 5.2:-12419465 | MS.gene64721:CDS |
| AGGCTGAAGCAGGGTCCAAC+TGG | 0.377077 | 5.2:-12419064 | MS.gene64721:CDS |
| GTGGGAGAGCTTTGGAAAGA+GGG | 0.397435 | 5.2:-12419155 | MS.gene64721:CDS |
| AGGTCAGAACCACGGGCTTA+AGG | 0.397457 | 5.2:-12419010 | MS.gene64721:CDS |
| CTAAGCAGTGATTTCATTTG+TGG | 0.399313 | 5.2:-12418735 | MS.gene64721:intron |
| ATGTGGGCCTGATGCTTATA+GGG | 0.399994 | 5.2:-12419405 | MS.gene64721:CDS |
| AAGATGGAGAGTGAAGTTTA+AGG | 0.402549 | 5.2:+12420802 | None:intergenic |
| AAAAGAAGTCGGTTATGATA+TGG | 0.403035 | 5.2:-12414333 | MS.gene64721:CDS |
| AATATTATCTCTGCATTCTC+AGG | 0.403373 | 5.2:+12419635 | None:intergenic |
| CTCCTCTGCTTAGTTTCCTC+AGG | 0.406909 | 5.2:+12414245 | None:intergenic |
| TATGTGGGCCTGATGCTTAT+AGG | 0.407996 | 5.2:-12419406 | MS.gene64721:CDS |
| GGTGGGAGAGCTTTGGAAAG+AGG | 0.410297 | 5.2:-12419156 | MS.gene64721:CDS |
| ACATCTTCCCTGTCTGAGAT+TGG | 0.413673 | 5.2:+12414056 | None:intergenic |
| TTACTGGAAGAAGTTGAATA+TGG | 0.415762 | 5.2:-12419371 | MS.gene64721:CDS |
| ATGAGGATGGTTTGTGAGGA+GGG | 0.419464 | 5.2:+12420739 | None:intergenic |
| TCACTGCTTAGGGCCACATC+TGG | 0.422985 | 5.2:+12418747 | None:intergenic |
| GTGAAAGAAGTAACGCTTCT+TGG | 0.424947 | 5.2:-12419131 | MS.gene64721:CDS |
| TTACAGGGGTTGTTGAAATT+GGG | 0.430909 | 5.2:+12420611 | None:intergenic |
| CATCCCAAAACAACTACTTT+CGG | 0.433324 | 5.2:+12419485 | None:intergenic |
| GGAAAGCGATGAGAAGGTTC+TGG | 0.433628 | 5.2:+12420692 | None:intergenic |
| ACACAACTTGTATTAGTTGA+AGG | 0.435385 | 5.2:-12414155 | MS.gene64721:CDS |
| ATACATTTACCTGCTCAATC+AGG | 0.436332 | 5.2:-12418855 | MS.gene64721:CDS |
| TGGAAGGCTAATGTCGCTAC+TGG | 0.450935 | 5.2:-12419530 | MS.gene64721:CDS |
| AGGAAAGTGTTACGACGAAC+AGG | 0.456568 | 5.2:-12414186 | MS.gene64721:CDS |
| GTGAATCTGAAACGCATCTC+GGG | 0.458313 | 5.2:+12418951 | None:intergenic |
| CACAAATGAAATCACTGCTT+AGG | 0.458907 | 5.2:+12418736 | None:intergenic |
| GGTTCTGACCTTTGATATGC+TGG | 0.462330 | 5.2:+12419022 | None:intergenic |
| GAATTCTGAGAGATCCGAAC+TGG | 0.464979 | 5.2:+12419293 | None:intergenic |
| GATACTGAAGCTCCTTCAAC+TGG | 0.466682 | 5.2:-12419761 | MS.gene64721:CDS |
| ACAAATGAAATCACTGCTTA+GGG | 0.474713 | 5.2:+12418737 | None:intergenic |
| ATAATATAAAGCAGTTCATC+TGG | 0.475681 | 5.2:+12418906 | None:intergenic |
| CGAATATATCATGAGACCTA+TGG | 0.476722 | 5.2:-12419737 | MS.gene64721:CDS |
| TCTCTCACAATGGATTCAAC+AGG | 0.479619 | 5.2:+12419179 | None:intergenic |
| TTGCTACTTCCTGATTGAGC+AGG | 0.481942 | 5.2:+12418846 | None:intergenic |
| GGCAAATCCCTATAAGCATC+AGG | 0.485324 | 5.2:+12419398 | None:intergenic |
| CAAGAGTGACAAGGGCCATC+GGG | 0.489925 | 5.2:-12414096 | MS.gene64721:CDS |
| TGATATATTCGACCAGTTGA+AGG | 0.490155 | 5.2:+12419749 | None:intergenic |
| TCTACAAGGGCATCACTCTT+CGG | 0.492794 | 5.2:-12413978 | MS.gene64721:CDS |
| CGATGAAGTGGCGGAGGGAA+GGG | 0.493107 | 5.2:+12420655 | None:intergenic |
| TCCTTCACTAAGTTTCCAGT+TGG | 0.501341 | 5.2:+12419049 | None:intergenic |
| AACTACTTTCGGAGGACGTA+GGG | 0.507112 | 5.2:+12419496 | None:intergenic |
| CGCTCTCTGCCTCTAGTGAA+AGG | 0.507564 | 5.2:+12419209 | None:intergenic |
| ATGCTGATATTAATCCAGTT+CGG | 0.509976 | 5.2:-12419307 | MS.gene64721:CDS |
| TCTGGATAATCTTTAGGGTG+TGG | 0.512551 | 5.2:+12418924 | None:intergenic |
| TGAACTGCTTTATATTATGC+GGG | 0.516942 | 5.2:-12418901 | MS.gene64721:CDS |
| GAAGTTGAATATGGCCAGAA+AGG | 0.519109 | 5.2:-12419362 | MS.gene64721:CDS |
| GTGAGGTTGGTTGATGATAG+AGG | 0.519781 | 5.2:+12420836 | None:intergenic |
| CGTGAATCTGAAACGCATCT+CGG | 0.527375 | 5.2:+12418950 | None:intergenic |
| TCAATCTGCCAATCTCAGAC+AGG | 0.530932 | 5.2:-12414064 | MS.gene64721:CDS |
| TTCTGGAAACGTTGATGAGA+GGG | 0.533036 | 5.2:+12420709 | None:intergenic |
| CTTGAGCGATGAAGTGGCGG+AGG | 0.533835 | 5.2:+12420649 | None:intergenic |
| CATCATCGACATAGTTTCTG+TGG | 0.538093 | 5.2:+12414270 | None:intergenic |
| AACACTTTCCTGTGCTCTCA+CGG | 0.540238 | 5.2:+12414198 | None:intergenic |
| ATATCAAAGGTCAGAACCAC+GGG | 0.540687 | 5.2:-12419017 | MS.gene64721:CDS |
| ATGAACTGCTTTATATTATG+CGG | 0.543355 | 5.2:-12418902 | MS.gene64721:CDS |
| TTGAAGAATCTGAGACAAGA+TGG | 0.543494 | 5.2:+12420786 | None:intergenic |
| GAGACTTTCCGTGAGAGCAC+AGG | 0.544489 | 5.2:-12414206 | MS.gene64721:CDS |
| CAGAAATGAAAGTGTGGCAG+AGG | 0.545396 | 5.2:-12419583 | MS.gene64721:CDS |
| TTTGTTGGAAAGCGATGAGA+AGG | 0.549943 | 5.2:+12420686 | None:intergenic |
| GCAAGAGTGACAAGGGCCAT+CGG | 0.550748 | 5.2:-12414097 | MS.gene64721:CDS |
| GTTTCTTAAAAGGAATTGGA+AGG | 0.554937 | 5.2:-12419546 | MS.gene64721:CDS |
| AAGTTGAATATGGCCAGAAA+GGG | 0.555341 | 5.2:-12419361 | MS.gene64721:CDS |
| GCGATGAAGTGGCGGAGGGA+AGG | 0.555405 | 5.2:+12420654 | None:intergenic |
| GGCGTTGAGCAGTGTGAGGT+TGG | 0.557634 | 5.2:+12420823 | None:intergenic |
| AACGTTCATTTGACATCCAT+AGG | 0.562542 | 5.2:+12419721 | None:intergenic |
| GCAAAATGCTTGAGAGAATG+AGG | 0.568043 | 5.2:-12418827 | MS.gene64721:CDS |
| TATCAGCATAAGTCTCCTCA+AGG | 0.568958 | 5.2:+12419321 | None:intergenic |
| ACATGTTTGCTTATAGCATG+AGG | 0.572834 | 5.2:-12414307 | MS.gene64721:CDS |
| ACAAAGCGAAATCCTGTGGT+TGG | 0.574953 | 5.2:-12414029 | MS.gene64721:CDS |
| AAAATGGTTGATGTTGTATG+TGG | 0.575431 | 5.2:-12419422 | MS.gene64721:CDS |
| TCGAAGTATTATTCCAGATG+TGG | 0.577594 | 5.2:-12418760 | MS.gene64721:CDS |
| AAGCTTGAGCGATGAAGTGG+CGG | 0.581337 | 5.2:+12420646 | None:intergenic |
| CTCTCCCACCTCTCTCACAA+TGG | 0.583858 | 5.2:+12419169 | None:intergenic |
| TGTTGAATCCATTGTGAGAG+AGG | 0.588576 | 5.2:-12419177 | MS.gene64721:CDS |
| AGAGGGCGTGAAGATGAGGA+TGG | 0.592092 | 5.2:+12420726 | None:intergenic |
| CATGTTTGCTTATAGCATGA+GGG | 0.592181 | 5.2:-12414306 | MS.gene64721:CDS |
| ATTAATTGGCAAGAGTGACA+AGG | 0.593336 | 5.2:-12414105 | MS.gene64721:CDS |
| TAACACTCTTCTTTCCCTTG+AGG | 0.594003 | 5.2:-12419336 | MS.gene64721:CDS |
| TCAACATAATCGCCAACCAC+AGG | 0.600128 | 5.2:+12414017 | None:intergenic |
| TGTATTGTACCTTTCACTAG+AGG | 0.601685 | 5.2:-12419218 | MS.gene64721:CDS |
| CTGAGGAAACTAAGCAGAGG+AGG | 0.601889 | 5.2:-12414244 | MS.gene64721:CDS |
| TTCAGATAACAAGATCTACA+AGG | 0.602317 | 5.2:-12413992 | MS.gene64721:CDS |
| GAGAAGCTTGAGCGATGAAG+TGG | 0.605336 | 5.2:+12420643 | None:intergenic |
| GGGTTGTTGAAATTGGGAAG+CGG | 0.606952 | 5.2:+12420617 | None:intergenic |
| AGGCTAATGTCGCTACTGGA+AGG | 0.608033 | 5.2:-12419526 | MS.gene64721:CDS |
| CAACTACTTTCGGAGGACGT+AGG | 0.608360 | 5.2:+12419495 | None:intergenic |
| GTTCTGGAAACGTTGATGAG+AGG | 0.609095 | 5.2:+12420708 | None:intergenic |
| TCAACTTCTTCCAGTAATCG+TGG | 0.609939 | 5.2:+12419377 | None:intergenic |
| ATCAGCATAAGTCTCCTCAA+GGG | 0.614806 | 5.2:+12419322 | None:intergenic |
| GAATCCATTGTGAGAGAGGT+GGG | 0.615431 | 5.2:-12419173 | MS.gene64721:CDS |
| CAATCTGCCAATCTCAGACA+GGG | 0.616817 | 5.2:-12414063 | MS.gene64721:CDS |
| ATCATCGACATAGTTTCTGT+GGG | 0.618344 | 5.2:+12414271 | None:intergenic |
| CTATGTCGATGATGTTCCTG+AGG | 0.625587 | 5.2:-12414261 | MS.gene64721:CDS |
| GATGAGGATGGTTTGTGAGG+AGG | 0.627793 | 5.2:+12420738 | None:intergenic |
| GATGAGAGGGCGTGAAGATG+AGG | 0.629719 | 5.2:+12420722 | None:intergenic |
| CATATCAAAGGTCAGAACCA+CGG | 0.636345 | 5.2:-12419018 | MS.gene64721:CDS |
| GAGCTTCAGTATCCGCCACA+AGG | 0.644626 | 5.2:+12419771 | None:intergenic |
| TTAAGGCGTTGAGCAGTGTG+AGG | 0.645466 | 5.2:+12420819 | None:intergenic |
| ATAATGCAGAAATGAAAGTG+TGG | 0.645760 | 5.2:-12419589 | MS.gene64721:CDS |
| TCCAACTGGAAACTTAGTGA+AGG | 0.647645 | 5.2:-12419050 | MS.gene64721:CDS |
| CAGCTACAATGATGCATCTG+TGG | 0.649965 | 5.2:-12419096 | MS.gene64721:CDS |
| TGAATCCATTGTGAGAGAGG+TGG | 0.653338 | 5.2:-12419174 | MS.gene64721:CDS |
| TTGAGCGATGAAGTGGCGGA+GGG | 0.653715 | 5.2:+12420650 | None:intergenic |
| TTGACTCTAGTAAAAGAAGT+CGG | 0.654807 | 5.2:-12414344 | MS.gene64721:CDS |
| TTAATTGGCAAGAGTGACAA+GGG | 0.656389 | 5.2:-12414104 | MS.gene64721:CDS |
| AAGTGTTACGACGAACAGGT+TGG | 0.662080 | 5.2:-12414182 | MS.gene64721:CDS |
| CCCAAAACAACTACTTTCGG+AGG | 0.662371 | 5.2:+12419488 | None:intergenic |
| ATCAGAAAACCTTAAGCCCG+TGG | 0.663982 | 5.2:+12419001 | None:intergenic |
| TAATACAAAGCGAAATCCTG+TGG | 0.667447 | 5.2:-12414033 | MS.gene64721:CDS |
| GATTGACAAATATTACCCGA+TGG | 0.668056 | 5.2:+12414081 | None:intergenic |
| TCAGATAACAAGATCTACAA+GGG | 0.670870 | 5.2:-12413991 | MS.gene64721:CDS |
| AAATGGTTGATGTTGTATGT+GGG | 0.688187 | 5.2:-12419421 | MS.gene64721:CDS |
| GAAGATGAGGATGGTTTGTG+AGG | 0.690427 | 5.2:+12420735 | None:intergenic |
| ATGCTTGAGAGAATGAGGCG+AGG | 0.692173 | 5.2:-12418822 | MS.gene64721:CDS |
| TTCCTGAGGAAACTAAGCAG+AGG | 0.694319 | 5.2:-12414247 | MS.gene64721:CDS |
| GAGGCGAGGATATACTCGAG+AGG | 0.697869 | 5.2:-12418808 | MS.gene64721:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 12413902 | 12420874 | 12413902 | ID=MS.gene64721 |
| chr5.2 | mRNA | 12413902 | 12420874 | 12413902 | ID=MS.gene64721.t1;Parent=MS.gene64721 |
| chr5.2 | exon | 12420615 | 12420874 | 12420615 | ID=MS.gene64721.t1.exon1;Parent=MS.gene64721.t1 |
| chr5.2 | CDS | 12420615 | 12420874 | 12420615 | ID=cds.MS.gene64721.t1;Parent=MS.gene64721.t1 |
| chr5.2 | exon | 12418736 | 12419793 | 12418736 | ID=MS.gene64721.t1.exon2;Parent=MS.gene64721.t1 |
| chr5.2 | CDS | 12418736 | 12419793 | 12418736 | ID=cds.MS.gene64721.t1;Parent=MS.gene64721.t1 |
| chr5.2 | exon | 12413902 | 12414404 | 12413902 | ID=MS.gene64721.t1.exon3;Parent=MS.gene64721.t1 |
| chr5.2 | CDS | 12413902 | 12414404 | 12413902 | ID=cds.MS.gene64721.t1;Parent=MS.gene64721.t1 |
| Gene Sequence |
| Protein sequence |
