Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65107.t1 | XP_003622954.1 | 89.4 | 179 | 19 | 0 | 5 | 183 | 40 | 218 | 3.10E-79 | 304.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65107.t1 | G7L1B6 | 89.4 | 179 | 19 | 0 | 5 | 183 | 40 | 218 | 2.2e-79 | 304.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene65107 | 0.829077 | 6.30E-55 | -1.69E-46 |
| MS.gene049910 | MS.gene65107 | 0.802439 | 5.61E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene65107 | 0.811452 | 6.95E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene65107 | 0.801518 | 8.67E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene65107 | 0.821307 | 4.33E-53 | -1.69E-46 |
| MS.gene050558 | MS.gene65107 | 0.807072 | 6.04E-50 | -1.69E-46 |
| MS.gene051662 | MS.gene65107 | 0.810653 | 1.04E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene65107 | 0.862996 | 3.41E-64 | -1.69E-46 |
| MS.gene051860 | MS.gene65107 | 0.801081 | 1.07E-48 | -1.69E-46 |
| MS.gene053836 | MS.gene65107 | 0.856179 | 3.83E-62 | -1.69E-46 |
| MS.gene054930 | MS.gene65107 | 0.829543 | 4.85E-55 | -1.69E-46 |
| MS.gene054942 | MS.gene65107 | 0.817683 | 2.90E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene65107 | 0.817817 | 2.71E-52 | -1.69E-46 |
| MS.gene055428 | MS.gene65107 | 0.819756 | 9.82E-53 | -1.69E-46 |
| MS.gene055712 | MS.gene65107 | 0.831943 | 1.25E-55 | -1.69E-46 |
| MS.gene056407 | MS.gene65107 | 0.814145 | 1.79E-51 | -1.69E-46 |
| MS.gene056875 | MS.gene65107 | 0.826644 | 2.42E-54 | -1.69E-46 |
| MS.gene057439 | MS.gene65107 | 0.812832 | 3.48E-51 | -1.69E-46 |
| MS.gene057448 | MS.gene65107 | 0.80588 | 1.08E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene65107 | 0.813632 | 2.32E-51 | -1.69E-46 |
| MS.gene057469 | MS.gene65107 | 0.808346 | 3.24E-50 | -1.69E-46 |
| MS.gene057750 | MS.gene65107 | 0.803101 | 4.09E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene65107 | 0.807098 | 5.97E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene65107 | 0.836564 | 8.69E-57 | -1.69E-46 |
| MS.gene05811 | MS.gene65107 | 0.827606 | 1.43E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene65107 | 0.840611 | 7.83E-58 | -1.69E-46 |
| MS.gene059518 | MS.gene65107 | 0.809494 | 1.84E-50 | -1.69E-46 |
| MS.gene059597 | MS.gene65107 | 0.805587 | 1.24E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene65107 | 0.81012 | 1.35E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65107.t1 | MTR_7g058760 | 89.385 | 179 | 19 | 0 | 5 | 183 | 40 | 218 | 3.07e-109 | 322 |
| MS.gene65107.t1 | MTR_7g058760 | 87.709 | 179 | 19 | 1 | 5 | 183 | 40 | 215 | 9.08e-105 | 310 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65107.t1 | AT5G48830 | 39.437 | 142 | 82 | 1 | 44 | 181 | 72 | 213 | 3.82e-22 | 93.2 |
| MS.gene65107.t1 | AT5G48830 | 39.437 | 142 | 82 | 1 | 44 | 181 | 72 | 213 | 4.36e-22 | 93.2 |
Find 36 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTCAAGCCGAGTGTATT+TGG | 0.164191 | 7.1:+42832969 | MS.gene65107:CDS |
| CGTGACAGCTCAGACATTTC+TGG | 0.224603 | 7.1:+42833218 | MS.gene65107:CDS |
| AATTCAAGCCGAGTGTATTT+GGG | 0.298989 | 7.1:+42832970 | MS.gene65107:CDS |
| AAGTATATTGCTCACTTATA+TGG | 0.317130 | 7.1:+42833127 | MS.gene65107:CDS |
| TCCAAAGTTAAGGTTACTTT+GGG | 0.362833 | 7.1:+42833501 | MS.gene65107:CDS |
| GTCCAAAGTTAAGGTTACTT+TGG | 0.374599 | 7.1:+42833500 | MS.gene65107:CDS |
| TTGCATTGGCATAGGAGCTT+TGG | 0.387344 | 7.1:+42833040 | MS.gene65107:CDS |
| TCCACTGGTTGTACAGAAAC+AGG | 0.392162 | 7.1:-42832818 | None:intergenic |
| TCTTTGTTGCTTCTATTGCC+TGG | 0.403195 | 7.1:+42832842 | MS.gene65107:CDS |
| TTGCCGAGGGTCCAAAGTTA+AGG | 0.405993 | 7.1:+42833491 | MS.gene65107:CDS |
| TCTAAATGCATTAGAGAAAG+AGG | 0.418471 | 7.1:+42833411 | MS.gene65107:CDS |
| CGGGTATAAGATTTGTCAAC+AGG | 0.422732 | 7.1:-42833161 | None:intergenic |
| TGGTGGAACAACATCTAATT+TGG | 0.428181 | 7.1:+42833073 | MS.gene65107:CDS |
| CTATGCCAATGCAATTTAAA+CGG | 0.439649 | 7.1:-42833031 | None:intergenic |
| TTGCAGTGCGGTGCAGCAAA+AGG | 0.455706 | 7.1:+42833333 | MS.gene65107:intron |
| GCCCAAAGTAACCTTAACTT+TGG | 0.458602 | 7.1:-42833502 | None:intergenic |
| AAAAGAAACTGATTAACAAC+TGG | 0.461741 | 7.1:-42833098 | None:intergenic |
| GCATTCCGTTTAAATTGCAT+TGG | 0.497490 | 7.1:+42833026 | MS.gene65107:CDS |
| ATATTGCTCACTTATATGGC+TGG | 0.502407 | 7.1:+42833131 | MS.gene65107:CDS |
| GGAGCTTTGGTTGACTCTGA+TGG | 0.508159 | 7.1:+42833053 | MS.gene65107:CDS |
| CGTTTAAATTGCATTGGCAT+AGG | 0.513020 | 7.1:+42833032 | MS.gene65107:CDS |
| TAACCTTAACTTTGGACCCT+CGG | 0.519078 | 7.1:-42833494 | None:intergenic |
| TTAGAGAAAGAGGCTTATGC+GGG | 0.522119 | 7.1:+42833421 | MS.gene65107:CDS |
| TCCTGTTTCTGTACAACCAG+TGG | 0.523707 | 7.1:+42832817 | MS.gene65107:CDS |
| AGTTTAAAAGCTGTTGCCGA+GGG | 0.560847 | 7.1:+42833478 | MS.gene65107:CDS |
| TTAAACGGAATGCCCGAAGA+CGG | 0.571659 | 7.1:-42833016 | None:intergenic |
| ATTCAAGCCGAGTGTATTTG+GGG | 0.587572 | 7.1:+42832971 | MS.gene65107:CDS |
| AGAAGCAACAAAGATTCCAC+TGG | 0.596892 | 7.1:-42832833 | None:intergenic |
| ATTAGAGAAAGAGGCTTATG+CGG | 0.602745 | 7.1:+42833420 | MS.gene65107:CDS |
| GAGTTTAAAAGCTGTTGCCG+AGG | 0.616763 | 7.1:+42833477 | MS.gene65107:CDS |
| GCTTTGGTTGACTCTGATGG+TGG | 0.626948 | 7.1:+42833056 | MS.gene65107:CDS |
| TCTGCAGCCCCAAATACACT+CGG | 0.654793 | 7.1:-42832978 | None:intergenic |
| GTCAAATTATGTATTAGATG+TGG | 0.662552 | 7.1:+42833366 | MS.gene65107:CDS |
| ACAGCTCAGACATTTCTGGT+AGG | 0.677195 | 7.1:+42833222 | MS.gene65107:CDS |
| TAGAGAAAGAGGCTTATGCG+GGG | 0.683596 | 7.1:+42833422 | MS.gene65107:CDS |
| AGAGAAAGAGGCTTATGCGG+GGG | 0.688620 | 7.1:+42833423 | MS.gene65107:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATTTTTGACATATGA+TGG | + | chr7.1:42833275-42833294 | MS.gene65107:intron | 15.0% |
| !!! | TATGTTATTTTGGTATGATT+TGG | + | chr7.1:42833596-42833615 | MS.gene65107:intron | 20.0% |
| ! | AAAAGAAACTGATTAACAAC+TGG | - | chr7.1:42833101-42833120 | None:intergenic | 25.0% |
| ! | AAGTATATTGCTCACTTATA+TGG | + | chr7.1:42833127-42833146 | MS.gene65107:CDS | 25.0% |
| ! | AGCAAAAGGAAAATATAGAA+AGG | - | chr7.1:42832896-42832915 | None:intergenic | 25.0% |
| ! | GTCAAATTATGTATTAGATG+TGG | + | chr7.1:42833366-42833385 | MS.gene65107:CDS | 25.0% |
| ! | TAAATGTTTCAAATGACTTC+TGG | + | chr7.1:42833649-42833668 | MS.gene65107:intron | 25.0% |
| !! | GTTAATCAGTTTCTTTTGAT+GGG | + | chr7.1:42833104-42833123 | MS.gene65107:CDS | 25.0% |
| !! | TGTTAATCAGTTTCTTTTGA+TGG | + | chr7.1:42833103-42833122 | MS.gene65107:CDS | 25.0% |
| !!! | AATTTTGACAAGTTGTAACT+TGG | + | chr7.1:42833619-42833638 | MS.gene65107:intron | 25.0% |
| CTATGCCAATGCAATTTAAA+CGG | - | chr7.1:42833034-42833053 | None:intergenic | 30.0% | |
| TAAAAATTACTCACCGTCTT+CGG | + | chr7.1:42833003-42833022 | MS.gene65107:CDS | 30.0% | |
| TCTAAATGCATTAGAGAAAG+AGG | + | chr7.1:42833411-42833430 | MS.gene65107:CDS | 30.0% | |
| ! | AGAATTGTTCTTTTGATCAC+GGG | - | chr7.1:42833183-42833202 | None:intergenic | 30.0% |
| ! | CAGAATTGTTCTTTTGATCA+CGG | - | chr7.1:42833184-42833203 | None:intergenic | 30.0% |
| ! | TCCAAAGTTAAGGTTACTTT+GGG | + | chr7.1:42833501-42833520 | MS.gene65107:CDS | 30.0% |
| !! | CTGCACATGTTATGTTATTT+TGG | + | chr7.1:42833586-42833605 | MS.gene65107:intron | 30.0% |
| !!! | GCTTGAATTTTTTCTGTGTA+AGG | - | chr7.1:42832959-42832978 | None:intergenic | 30.0% |
| AAAAATTACTCACCGTCTTC+GGG | + | chr7.1:42833004-42833023 | MS.gene65107:CDS | 35.0% | |
| AAATTCAAGCCGAGTGTATT+TGG | + | chr7.1:42832969-42832988 | MS.gene65107:CDS | 35.0% | |
| AATTCAAGCCGAGTGTATTT+GGG | + | chr7.1:42832970-42832989 | MS.gene65107:CDS | 35.0% | |
| ACAACCAAATTCATGATACC+AGG | - | chr7.1:42832863-42832882 | None:intergenic | 35.0% | |
| ATATTGCTCACTTATATGGC+TGG | + | chr7.1:42833131-42833150 | MS.gene65107:CDS | 35.0% | |
| ATTAGAGAAAGAGGCTTATG+CGG | + | chr7.1:42833420-42833439 | MS.gene65107:CDS | 35.0% | |
| ATTGCCTGGTATCATGAATT+TGG | + | chr7.1:42832856-42832875 | MS.gene65107:intron | 35.0% | |
| GAAATACTTTCTGCAGCAAA+AGG | - | chr7.1:42832910-42832929 | None:intergenic | 35.0% | |
| GCATTCCGTTTAAATTGCAT+TGG | + | chr7.1:42833026-42833045 | MS.gene65107:CDS | 35.0% | |
| GTAAGGGTAAGAAAGTCAAA+TGG | + | chr7.1:42833244-42833263 | MS.gene65107:intron | 35.0% | |
| GTCCAAAGTTAAGGTTACTT+TGG | + | chr7.1:42833500-42833519 | MS.gene65107:CDS | 35.0% | |
| TGGTGGAACAACATCTAATT+TGG | + | chr7.1:42833073-42833092 | MS.gene65107:CDS | 35.0% | |
| ! | CGTTTAAATTGCATTGGCAT+AGG | + | chr7.1:42833032-42833051 | MS.gene65107:CDS | 35.0% |
| ! | GCAACAGCTTTTAAACTCAA+GGG | - | chr7.1:42833475-42833494 | None:intergenic | 35.0% |
| AGAAGCAACAAAGATTCCAC+TGG | - | chr7.1:42832836-42832855 | None:intergenic | 40.0% | |
| AGTTTAAAAGCTGTTGCCGA+GGG | + | chr7.1:42833478-42833497 | MS.gene65107:CDS | 40.0% | |
| ATTCAAGCCGAGTGTATTTG+GGG | + | chr7.1:42832971-42832990 | MS.gene65107:CDS | 40.0% | |
| CGGGTATAAGATTTGTCAAC+AGG | - | chr7.1:42833164-42833183 | None:intergenic | 40.0% | |
| GCCCAAAGTAACCTTAACTT+TGG | - | chr7.1:42833505-42833524 | None:intergenic | 40.0% | |
| TAACCTTAACTTTGGACCCT+CGG | - | chr7.1:42833497-42833516 | None:intergenic | 40.0% | |
| TTAGAGAAAGAGGCTTATGC+GGG | + | chr7.1:42833421-42833440 | MS.gene65107:CDS | 40.0% | |
| ! | AGGGTCTTTTTGCAGATTGA+AGG | - | chr7.1:42833456-42833475 | None:intergenic | 40.0% |
| ! | CTTTTGTCTCTTTTGCAGTG+CGG | + | chr7.1:42833321-42833340 | MS.gene65107:intron | 40.0% |
| ! | GGCAACAGCTTTTAAACTCA+AGG | - | chr7.1:42833476-42833495 | None:intergenic | 40.0% |
| ! | TCTTTGTTGCTTCTATTGCC+TGG | + | chr7.1:42832842-42832861 | MS.gene65107:CDS | 40.0% |
| !! | CAGACATTTCTGGTAGGTAA+GGG | + | chr7.1:42833228-42833247 | MS.gene65107:intron | 40.0% |
| !! | TCAGACATTTCTGGTAGGTA+AGG | + | chr7.1:42833227-42833246 | MS.gene65107:intron | 40.0% |
| !! | TTTTCTGCAAGTTGAGGAAG+AGG | + | chr7.1:42833528-42833547 | MS.gene65107:CDS | 40.0% |
| GAGTTTAAAAGCTGTTGCCG+AGG | + | chr7.1:42833477-42833496 | MS.gene65107:CDS | 45.0% | |
| TAGAGAAAGAGGCTTATGCG+GGG | + | chr7.1:42833422-42833441 | MS.gene65107:CDS | 45.0% | |
| TTAAACGGAATGCCCGAAGA+CGG | - | chr7.1:42833019-42833038 | None:intergenic | 45.0% | |
| ! | TCCACTGGTTGTACAGAAAC+AGG | - | chr7.1:42832821-42832840 | None:intergenic | 45.0% |
| ! | TGCAAGTTGAGGAAGAGGTA+GGG | + | chr7.1:42833533-42833552 | MS.gene65107:intron | 45.0% |
| ! | TTGCATTGGCATAGGAGCTT+TGG | + | chr7.1:42833040-42833059 | MS.gene65107:CDS | 45.0% |
| !! | ACAGCTCAGACATTTCTGGT+AGG | + | chr7.1:42833222-42833241 | MS.gene65107:CDS | 45.0% |
| !! | TCCTGTTTCTGTACAACCAG+TGG | + | chr7.1:42832817-42832836 | MS.gene65107:CDS | 45.0% |
| AGAGAAAGAGGCTTATGCGG+GGG | + | chr7.1:42833423-42833442 | MS.gene65107:CDS | 50.0% | |
| CGTGACAGCTCAGACATTTC+TGG | + | chr7.1:42833218-42833237 | MS.gene65107:CDS | 50.0% | |
| TCTGCAGCCCCAAATACACT+CGG | - | chr7.1:42832981-42833000 | None:intergenic | 50.0% | |
| TTGCCGAGGGTCCAAAGTTA+AGG | + | chr7.1:42833491-42833510 | MS.gene65107:CDS | 50.0% | |
| ! | CTGCAAGTTGAGGAAGAGGT+AGG | + | chr7.1:42833532-42833551 | MS.gene65107:intron | 50.0% |
| ! | GCTTTGGTTGACTCTGATGG+TGG | + | chr7.1:42833056-42833075 | MS.gene65107:CDS | 50.0% |
| ! | GGAGCTTTGGTTGACTCTGA+TGG | + | chr7.1:42833053-42833072 | MS.gene65107:CDS | 50.0% |
| !!! | GGCTGCTTTTCTGCAAGTTG+AGG | + | chr7.1:42833522-42833541 | MS.gene65107:CDS | 50.0% |
| TTGCAGTGCGGTGCAGCAAA+AGG | + | chr7.1:42833333-42833352 | MS.gene65107:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 42832810 | 42833728 | 42832810 | ID=MS.gene65107 |
| chr7.1 | mRNA | 42832810 | 42833728 | 42832810 | ID=MS.gene65107.t1;Parent=MS.gene65107 |
| chr7.1 | exon | 42832810 | 42832863 | 42832810 | ID=MS.gene65107.t1.exon1;Parent=MS.gene65107.t1 |
| chr7.1 | CDS | 42832810 | 42832863 | 42832810 | ID=cds.MS.gene65107.t1;Parent=MS.gene65107.t1 |
| chr7.1 | exon | 42832966 | 42833243 | 42832966 | ID=MS.gene65107.t1.exon2;Parent=MS.gene65107.t1 |
| chr7.1 | CDS | 42832966 | 42833243 | 42832966 | ID=cds.MS.gene65107.t1;Parent=MS.gene65107.t1 |
| chr7.1 | exon | 42833339 | 42833549 | 42833339 | ID=MS.gene65107.t1.exon3;Parent=MS.gene65107.t1 |
| chr7.1 | CDS | 42833339 | 42833549 | 42833339 | ID=cds.MS.gene65107.t1;Parent=MS.gene65107.t1 |
| chr7.1 | exon | 42833687 | 42833728 | 42833687 | ID=MS.gene65107.t1.exon4;Parent=MS.gene65107.t1 |
| chr7.1 | CDS | 42833687 | 42833728 | 42833687 | ID=cds.MS.gene65107.t1;Parent=MS.gene65107.t1 |
| Gene Sequence |
| Protein sequence |
