AlfalfaGEDB | MS.gene65284

M. sativa cultivar XinJiangDaYe / MS.gene65284

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF00332.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65284.t1	XP_003594771.2	96.2	316	12	0	10	325	74	389	1.30E-175	625.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65284.t1	P23535	75.6	316	75	2	11	325	1	315	2.4e-141	503.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65284.t1	G7IG01	96.2	316	12	0	10	325	20	335	9.6e-176	625.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049993	MS.gene65284	0.804062	2.59E-49	-1.69E-46
MS.gene050426	MS.gene65284	-0.820552	6.46E-53	-1.69E-46
MS.gene051525	MS.gene65284	0.811448	6.97E-51	-1.69E-46
MS.gene052011	MS.gene65284	-0.839631	1.41E-57	-1.69E-46
MS.gene052077	MS.gene65284	0.82735	1.64E-54	-1.69E-46
MS.gene052134	MS.gene65284	0.806138	9.52E-50	-1.69E-46
MS.gene052135	MS.gene65284	0.818178	2.24E-52	-1.69E-46
MS.gene052589	MS.gene65284	0.835141	1.99E-56	-1.69E-46
MS.gene052590	MS.gene65284	0.841888	3.62E-58	-1.69E-46
MS.gene053330	MS.gene65284	0.82173	3.46E-53	-1.69E-46
MS.gene054102	MS.gene65284	0.842337	2.75E-58	-1.69E-46
MS.gene054110	MS.gene65284	0.817267	3.60E-52	-1.69E-46
MS.gene054183	MS.gene65284	0.825402	4.78E-54	-1.69E-46
MS.gene054349	MS.gene65284	0.805513	1.29E-49	-1.69E-46
MS.gene05553	MS.gene65284	0.825428	4.71E-54	-1.69E-46
MS.gene056981	MS.gene65284	0.807518	4.86E-50	-1.69E-46
MS.gene057833	MS.gene65284	0.818212	2.21E-52	-1.69E-46
MS.gene057843	MS.gene65284	0.813677	2.27E-51	-1.69E-46
MS.gene059011	MS.gene65284	0.838137	3.44E-57	-1.69E-46
MS.gene059420	MS.gene65284	0.836673	8.15E-57	-1.69E-46
MS.gene059958	MS.gene65284	0.802577	5.25E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65284.t1	MTR_2g034440	94.118	323	19	0	3	325	13	335	0.0	628
MS.gene65284.t1	MTR_2g034470	95.253	316	14	1	10	325	28	342	0.0	613
MS.gene65284.t1	MTR_2g034470	95.130	308	14	1	18	325	1	307	0.0	594
MS.gene65284.t1	MTR_2g034480	75.776	322	77	1	4	325	19	339	0.0	504
MS.gene65284.t1	MTR_4g076570	54.603	315	138	4	12	324	22	333	2.37e-115	338
MS.gene65284.t1	MTR_1g052430	53.125	320	142	5	7	321	24	340	1.72e-112	332
MS.gene65284.t1	MTR_4g076470	52.698	315	139	5	12	325	23	328	6.55e-106	313
MS.gene65284.t1	MTR_4g076255	52.500	320	139	8	9	325	21	330	2.70e-105	311
MS.gene65284.t1	MTR_4g076440	50.794	315	145	5	12	325	23	328	2.16e-100	298
MS.gene65284.t1	MTR_4g076490	51.274	314	142	6	10	321	95	399	2.68e-100	301
MS.gene65284.t1	MTR_4g076500	48.428	318	154	5	12	325	78	389	2.10e-99	298
MS.gene65284.t1	MTR_4g076430	50.476	315	146	5	12	325	23	328	1.11e-98	294
MS.gene65284.t1	MTR_7g081350	41.311	305	169	6	12	312	27	325	1.00e-72	228
MS.gene65284.t1	MTR_6g088885	37.658	316	185	7	9	315	24	336	4.14e-69	224
MS.gene65284.t1	MTR_4g088155	37.771	323	183	10	6	313	17	336	6.19e-66	214
MS.gene65284.t1	MTR_7g081370	39.404	302	166	5	12	312	28	313	2.51e-65	209
MS.gene65284.t1	MTR_4g069940	38.245	319	186	7	3	312	20	336	1.34e-64	211
MS.gene65284.t1	MTR_1g056640	37.383	321	189	8	7	318	34	351	1.97e-64	211
MS.gene65284.t1	MTR_8g012400	35.077	325	197	8	6	318	23	345	3.48e-64	210
MS.gene65284.t1	MTR_3g080410	38.558	319	185	7	9	318	23	339	1.53e-63	205
MS.gene65284.t1	MTR_7g053500	34.688	320	195	8	11	318	25	342	2.58e-63	207
MS.gene65284.t1	MTR_4g052970	38.291	316	183	8	9	315	26	338	7.86e-63	204
MS.gene65284.t1	MTR_5g085580	37.658	316	186	7	12	318	25	338	8.90e-62	204
MS.gene65284.t1	MTR_4g010200	36.449	321	194	7	13	325	34	352	2.00e-60	198
MS.gene65284.t1	MTR_3g116510	34.579	321	199	7	1	312	17	335	3.91e-60	199
MS.gene65284.t1	MTR_1g077990	37.179	312	185	8	12	315	31	339	2.08e-59	196
MS.gene65284.t1	MTR_2g086530	35.625	320	184	10	9	315	5	315	3.44e-58	192
MS.gene65284.t1	MTR_2g086530	35.583	326	187	11	4	315	20	336	3.74e-58	192
MS.gene65284.t1	MTR_3g083580	34.234	333	196	6	9	325	23	348	6.35e-58	194
MS.gene65284.t1	MTR_4g084480	36.190	315	189	8	10	315	30	341	1.49e-57	191
MS.gene65284.t1	MTR_4g084480	36.190	315	189	8	10	315	23	334	1.56e-57	191
MS.gene65284.t1	MTR_4g084480	36.190	315	189	8	10	315	30	341	1.71e-57	191
MS.gene65284.t1	MTR_1g007810	38.730	315	175	11	12	315	16	323	8.07e-57	188
MS.gene65284.t1	MTR_4g114850	34.194	310	191	8	13	312	27	333	3.44e-56	186
MS.gene65284.t1	MTR_1g031900	37.855	317	184	7	10	315	22	336	5.82e-56	189
MS.gene65284.t1	MTR_1g031900	37.855	317	184	7	10	315	22	336	6.02e-56	189
MS.gene65284.t1	MTR_1g022160	35.256	312	190	8	12	313	24	333	6.66e-56	187
MS.gene65284.t1	MTR_4g114850	34.194	310	191	8	13	312	27	333	7.15e-56	186
MS.gene65284.t1	MTR_4g114850	33.654	312	196	7	13	315	27	336	8.17e-56	186
MS.gene65284.t1	MTR_3g065460	37.778	315	183	7	12	315	24	336	2.16e-55	187
MS.gene65284.t1	MTR_1g022160	35.256	312	190	8	12	313	24	333	4.50e-55	186
MS.gene65284.t1	MTR_1g022160	35.256	312	190	8	12	313	24	333	5.32e-55	186
MS.gene65284.t1	MTR_1g022160	35.256	312	190	8	12	313	24	333	5.47e-55	186
MS.gene65284.t1	MTR_4g124440	36.246	309	186	7	23	323	40	345	1.73e-54	185
MS.gene65284.t1	MTR_5g078200	38.413	315	181	7	12	315	23	335	4.01e-54	187
MS.gene65284.t1	MTR_7g026340	36.943	314	180	13	12	315	23	328	1.66e-53	182
MS.gene65284.t1	MTR_2g027560	33.750	320	201	6	10	319	26	344	4.27e-53	181
MS.gene65284.t1	MTR_4g132280	34.700	317	194	6	7	312	40	354	1.02e-52	181
MS.gene65284.t1	MTR_8g085720	33.533	334	208	7	3	325	17	347	1.33e-52	180
MS.gene65284.t1	MTR_5g078200	38.487	304	174	7	23	315	1	302	2.62e-52	182
MS.gene65284.t1	MTR_1g010120	34.951	309	192	6	12	312	26	333	2.71e-51	176
MS.gene65284.t1	MTR_6g032820	34.062	320	197	6	12	319	26	343	2.82e-51	176
MS.gene65284.t1	MTR_8g102340	34.069	317	163	9	8	315	24	303	9.19e-51	175
MS.gene65284.t1	MTR_8g092070	31.677	322	210	6	12	325	34	353	1.74e-50	176
MS.gene65284.t1	MTR_2g019345	34.375	320	199	8	4	315	16	332	1.77e-50	172
MS.gene65284.t1	MTR_4g112025	32.722	327	209	6	3	319	22	347	2.49e-50	175
MS.gene65284.t1	MTR_7g085390	34.161	322	197	8	12	322	26	343	9.09e-50	172
MS.gene65284.t1	MTR_8g012400	34.586	266	160	8	65	318	2	265	4.79e-49	168
MS.gene65284.t1	MTR_1g053320	32.209	326	211	6	4	319	22	347	1.70e-47	166
MS.gene65284.t1	MTR_7g053500	33.835	266	162	8	65	318	2	265	6.67e-47	162
MS.gene65284.t1	MTR_3g108600	33.766	308	178	6	12	312	31	319	8.12e-46	162
MS.gene65284.t1	MTR_3g095050	31.692	325	204	9	12	325	34	351	4.80e-45	160
MS.gene65284.t1	MTR_3g031770	30.159	315	211	6	12	319	27	339	1.42e-43	156
MS.gene65284.t1	MTR_4g083500	35.093	322	192	12	5	315	37	352	2.49e-42	154
MS.gene65284.t1	MTR_5g081720	33.219	292	178	11	38	319	63	347	3.81e-37	137
MS.gene65284.t1	MTR_4g134280	35.345	232	140	6	99	323	2	230	1.26e-35	132
MS.gene65284.t1	MTR_3g116510	36.139	202	119	6	120	312	1	201	1.37e-35	131
MS.gene65284.t1	MTR_4g036680	49.474	95	45	3	229	321	80	173	3.51e-23	95.9
MS.gene65284.t1	MTR_4g036680	61.429	70	26	1	6	74	15	84	9.07e-20	86.7
MS.gene65284.t1	MTR_4g036775	49.474	95	45	3	229	321	80	173	1.98e-22	94.0
MS.gene65284.t1	MTR_4g036775	61.429	70	26	1	6	74	15	84	1.67e-20	88.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65284.t1	AT4G16260	59.816	326	122	5	3	325	15	334	2.30e-138	395
MS.gene65284.t1	AT4G16260	60.450	311	114	5	18	325	1	305	1.07e-131	378
MS.gene65284.t1	AT3G57270	51.090	321	150	4	8	325	23	339	5.40e-111	326
MS.gene65284.t1	AT3G57240	51.543	324	145	7	4	325	27	340	6.00e-105	311
MS.gene65284.t1	AT3G57260	48.607	323	155	5	5	325	25	338	2.48e-101	301
MS.gene65284.t1	AT3G57260	48.607	323	155	5	5	325	41	354	3.06e-101	301
MS.gene65284.t1	AT5G20390	38.037	326	189	7	2	321	23	341	2.99e-71	224
MS.gene65284.t1	AT5G56590	37.855	317	185	7	9	315	23	337	3.82e-71	229
MS.gene65284.t1	AT5G20340	38.889	324	187	6	3	321	34	351	1.32e-70	223
MS.gene65284.t1	AT5G20330	38.820	322	186	6	5	321	27	342	4.08e-68	217
MS.gene65284.t1	AT2G16230	40.125	319	180	7	6	315	19	335	7.07e-68	221
MS.gene65284.t1	AT4G29360	41.139	316	174	7	9	315	24	336	4.95e-66	216
MS.gene65284.t1	AT4G29360	41.139	316	174	7	9	315	34	346	5.22e-66	217
MS.gene65284.t1	AT1G77790	38.291	316	180	7	8	315	26	334	1.09e-64	208
MS.gene65284.t1	AT4G34480	38.750	320	185	7	2	312	16	333	8.77e-64	206
MS.gene65284.t1	AT2G26600	36.025	322	194	8	13	325	35	353	3.24e-63	205
MS.gene65284.t1	AT4G34480	38.750	320	185	7	2	312	16	333	2.48e-62	206
MS.gene65284.t1	AT2G05790	37.107	318	189	7	10	318	23	338	4.64e-62	204
MS.gene65284.t1	AT1G33220	37.748	302	177	6	5	301	27	322	5.49e-62	201
MS.gene65284.t1	AT5G20560	37.338	308	183	6	12	314	34	336	5.62e-62	201
MS.gene65284.t1	AT3G07320	34.169	319	194	9	13	318	26	341	7.33e-62	204
MS.gene65284.t1	AT1G32860	35.890	326	194	9	11	325	28	349	1.29e-61	202
MS.gene65284.t1	AT5G42720	36.335	322	195	7	3	315	17	337	1.39e-61	202
MS.gene65284.t1	AT2G27500	35.535	318	194	7	9	317	26	341	9.75e-61	199
MS.gene65284.t1	AT2G27500	35.535	318	194	7	9	317	26	341	1.63e-60	198
MS.gene65284.t1	AT4G26830	35.404	322	190	9	6	315	23	338	2.49e-59	197
MS.gene65284.t1	AT5G55180	36.593	317	188	8	12	318	27	340	2.80e-59	197
MS.gene65284.t1	AT4G26830	35.759	316	185	9	12	315	25	334	2.87e-59	197
MS.gene65284.t1	AT5G55180	36.593	317	188	8	12	318	27	340	3.63e-59	197
MS.gene65284.t1	AT3G15800	36.102	313	188	8	13	315	44	354	5.98e-59	194
MS.gene65284.t1	AT1G77780	36.482	307	185	7	13	315	25	325	8.17e-59	193
MS.gene65284.t1	AT2G01630	37.460	315	184	6	12	315	23	335	3.56e-58	192
MS.gene65284.t1	AT5G42100	37.539	317	185	8	10	315	25	339	7.99e-58	192
MS.gene65284.t1	AT5G42100	37.539	317	185	8	10	315	25	339	9.12e-58	192
MS.gene65284.t1	AT2G01630	37.460	315	184	6	12	315	23	335	1.29e-57	192
MS.gene65284.t1	AT2G01630	37.460	315	184	6	12	315	23	335	6.27e-57	192
MS.gene65284.t1	AT5G58090	33.861	316	200	5	6	313	16	330	7.46e-56	188
MS.gene65284.t1	AT4G14080	33.229	319	198	9	9	315	39	354	9.61e-56	188
MS.gene65284.t1	AT1G66250	35.737	319	193	6	9	317	29	345	1.20e-55	189
MS.gene65284.t1	AT3G23770	34.268	321	195	9	9	315	34	352	3.20e-55	187
MS.gene65284.t1	AT1G30080	34.161	322	202	7	2	315	26	345	5.77e-54	181
MS.gene65284.t1	AT3G61810	34.462	325	194	8	12	325	55	371	7.54e-54	181
MS.gene65284.t1	AT1G30080	34.161	322	202	7	2	315	26	345	1.04e-53	181
MS.gene65284.t1	AT4G18340	35.241	332	194	10	7	325	29	352	1.63e-53	180
MS.gene65284.t1	AT4G18340	35.241	332	194	10	7	325	29	352	2.00e-53	180
MS.gene65284.t1	AT1G11820	33.654	312	194	6	12	312	44	353	7.61e-53	178
MS.gene65284.t1	AT1G11820	33.654	312	194	6	12	312	44	353	9.15e-52	178
MS.gene65284.t1	AT1G11820	33.654	312	194	6	12	312	44	353	2.30e-51	177
MS.gene65284.t1	AT5G18220	33.333	321	202	7	10	322	28	344	3.51e-51	176
MS.gene65284.t1	AT3G04010	34.259	324	199	8	12	325	33	352	2.26e-50	174
MS.gene65284.t1	AT5G24318	34.056	323	191	10	3	312	21	334	7.15e-50	170
MS.gene65284.t1	AT4G17180	33.229	319	197	8	10	319	23	334	1.12e-49	172
MS.gene65284.t1	AT4G31140	33.333	309	197	5	12	312	27	334	1.28e-49	172
MS.gene65284.t1	AT5G58480	31.988	322	209	6	12	325	26	345	4.85e-49	171
MS.gene65284.t1	AT5G24318	34.056	323	191	10	3	312	21	334	4.94e-49	170
MS.gene65284.t1	AT3G55430	36.943	314	178	15	10	312	27	331	5.12e-49	170
MS.gene65284.t1	AT5G24318	34.056	323	191	10	3	312	21	334	6.24e-49	170
MS.gene65284.t1	AT3G13560	33.951	324	201	7	3	315	17	338	1.24e-48	170
MS.gene65284.t1	AT3G13560	33.951	324	201	7	3	315	17	338	1.24e-48	170
MS.gene65284.t1	AT3G13560	33.951	324	201	7	3	315	17	338	1.24e-48	170
MS.gene65284.t1	AT3G13560	33.951	324	201	7	3	315	17	338	1.24e-48	170
MS.gene65284.t1	AT3G13560	33.951	324	201	7	3	315	17	338	1.24e-48	170
MS.gene65284.t1	AT2G27500	35.472	265	160	7	62	317	1	263	6.46e-48	163
MS.gene65284.t1	AT2G26600	36.508	252	149	7	73	315	1	250	7.13e-48	163
MS.gene65284.t1	AT2G19440	33.333	321	200	9	12	322	21	337	1.05e-47	167
MS.gene65284.t1	AT2G19440	33.333	321	200	9	12	322	21	337	1.05e-47	167
MS.gene65284.t1	AT3G24330	31.692	325	209	7	6	319	33	355	1.44e-47	167
MS.gene65284.t1	AT1G64760	32.710	321	202	9	12	322	25	341	1.57e-46	164
MS.gene65284.t1	AT1G64760	32.710	321	202	9	12	322	25	341	1.57e-46	164
MS.gene65284.t1	AT5G64790	30.844	308	204	6	13	312	29	335	9.10e-44	157
MS.gene65284.t1	AT5G20870	33.227	313	188	10	30	325	48	356	1.65e-43	156
MS.gene65284.t1	AT3G46570	34.268	321	192	13	7	315	22	335	2.91e-43	152
MS.gene65284.t1	AT2G39640	32.710	321	194	13	5	315	23	331	4.02e-41	150
MS.gene65284.t1	AT3G55780	32.024	331	200	13	12	325	31	353	1.46e-39	143
MS.gene65284.t1	AT4G34480	39.189	222	124	7	100	312	3	222	2.42e-36	135
MS.gene65284.t1	AT5G24318	34.158	202	114	7	123	312	1	195	1.46e-26	107

Find 62 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTATTGAGACTTATATATT+TGG	0.120186	2.2:+54582800	MS.gene65284:CDS
CCTGAGTTGGAGAAACATTT+TGG	0.160115	2.2:+54582848	MS.gene65284:CDS
ACAAAGCATCAAATAAATTT+TGG	0.183195	2.2:-54582604	None:intergenic
GATCATATAGCCTCATTCTT+TGG	0.245537	2.2:-54582055	None:intergenic
AAGTGATGTGAGGTCATATT+TGG	0.246765	2.2:+54582427	MS.gene65284:CDS
CTTCTCCAAATGTTGTAGTT+AGG	0.253024	2.2:+54582564	MS.gene65284:CDS
TATTGAAGTGAGATGTCTTT+TGG	0.257327	2.2:-54582530	None:intergenic
TATACTAAGTACCCTATAAT+TGG	0.264552	2.2:-54582452	None:intergenic
TCATATTTGGATCCAATTAT+AGG	0.278564	2.2:+54582440	MS.gene65284:CDS
CATATTTGGATCCAATTATA+GGG	0.326287	2.2:+54582441	MS.gene65284:CDS
AATTTGATTAGGCATGTTAA+AGG	0.330111	2.2:+54582755	MS.gene65284:CDS
CTATTGAGACTTATATATTT+GGG	0.343334	2.2:+54582801	MS.gene65284:CDS
AAATTTATTTGATGCTTTGT+TGG	0.344765	2.2:+54582607	MS.gene65284:CDS
ATTCGCAAAATTTGTTCTTC+CGG	0.345878	2.2:+54582283	MS.gene65284:CDS
GCAATTGATAATACTGGAAT+TGG	0.351863	2.2:+54582644	MS.gene65284:CDS
TCACAAGCAGGTGCTCAAAT+AGG	0.357040	2.2:+54581957	MS.gene65284:CDS
GAGTAAATATTTGCAAATAA+AGG	0.360502	2.2:-54582485	None:intergenic
CATGCTGCAATTGATAATAC+TGG	0.383359	2.2:+54582638	MS.gene65284:CDS
TTTATCTTGATAATTTGATT+AGG	0.386954	2.2:+54582744	MS.gene65284:CDS
AATTGAGAATTGCCACCAAC+TGG	0.394770	2.2:-54582263	None:intergenic
TTCTCCAAATGTTGTAGTTA+GGG	0.406751	2.2:+54582565	MS.gene65284:CDS
CCATCCCTAACTACAACATT+TGG	0.407887	2.2:-54582569	None:intergenic
ACAAAGACATTGCAAATCAA+TGG	0.408391	2.2:+54582168	MS.gene65284:CDS
TTGATTTGCAATGTCTTTGT+TGG	0.409434	2.2:-54582165	None:intergenic
GCTTCAAGAGCATCTTGGTT+TGG	0.411205	2.2:-54582077	None:intergenic
CCAAATATATAAGTCTCAAT+AGG	0.425263	2.2:-54582800	None:intergenic
GCTCAAATAGGTGTTTGTTA+TGG	0.437286	2.2:+54581969	MS.gene65284:CDS
CCAAAATGTTTCTCCAACTC+AGG	0.437816	2.2:-54582848	None:intergenic
ACTTCGTATCCGCCGTCTAA+AGG	0.439251	2.2:+54582395	MS.gene65284:CDS
TTCACGTTGTGATGGTAGAT+TGG	0.447086	2.2:-54582003	None:intergenic
TTGTAGTTAGGGATGGTTCA+AGG	0.447329	2.2:+54582576	MS.gene65284:CDS
AAATCTTCACGATCAAATCA+AGG	0.476242	2.2:+54582340	MS.gene65284:CDS
CAAAGACATTGCAAATCAAT+GGG	0.489896	2.2:+54582169	MS.gene65284:CDS
TCTGGCATAGAGCTTATGTT+AGG	0.490580	2.2:+54582110	MS.gene65284:CDS
TGTAGTTAGGGATGGTTCAA+GGG	0.509069	2.2:+54582577	MS.gene65284:CDS
TGATTAGGCATGTTAAAGGT+GGG	0.513293	2.2:+54582759	MS.gene65284:CDS
GGTGGGACACCAATGAGGTC+TGG	0.514579	2.2:+54582776	MS.gene65284:CDS
CCAAATGTTGTAGTTAGGGA+TGG	0.514884	2.2:+54582569	MS.gene65284:CDS
AAGGTTGTTGTGTCTGAAAG+TGG	0.522952	2.2:+54582674	MS.gene65284:CDS
CTTGAAGCACTTAGAAACTC+TGG	0.524132	2.2:+54582092	MS.gene65284:CDS
AATAGGTGTTTGTTATGGCA+TGG	0.529222	2.2:+54581974	MS.gene65284:CDS
TAAGTGCTTCAAGAGCATCT+TGG	0.536197	2.2:-54582082	None:intergenic
GGTGTTTGTTATGGCATGGA+AGG	0.540427	2.2:+54581978	MS.gene65284:CDS
TCAATAGGTCCAGACCTCAT+TGG	0.541707	2.2:-54582785	None:intergenic
TTGATTAGGCATGTTAAAGG+TGG	0.546182	2.2:+54582758	MS.gene65284:CDS
AACGAAGTTAATCCAGTTGG+TGG	0.557119	2.2:+54582251	MS.gene65284:CDS
CAAACAACATCCAAAGAATG+AGG	0.557727	2.2:+54582045	MS.gene65284:CDS
TCTATTGCTTCACGTTGTGA+TGG	0.567520	2.2:-54582011	None:intergenic
CGGATACGAAGTTTCTATCA+TGG	0.570657	2.2:-54582384	None:intergenic
GAAAGTGGTTGGCCTTCAGA+TGG	0.571089	2.2:+54582689	MS.gene65284:CDS
AGTGGTTGGCCTTCAGATGG+TGG	0.591010	2.2:+54582692	MS.gene65284:CDS
GAATAAGAAGAATCCTGAGT+TGG	0.594707	2.2:+54582835	MS.gene65284:CDS
TTGTTGTGTCTGAAAGTGGT+TGG	0.599923	2.2:+54582678	MS.gene65284:CDS
GTAGTTAGGGATGGTTCAAG+GGG	0.619346	2.2:+54582578	MS.gene65284:CDS
TATCATGGTCATGTCAATAG+CGG	0.631104	2.2:-54582369	None:intergenic
AAAATCAAATACATCGCGGT+CGG	0.638139	2.2:+54582227	MS.gene65284:CDS
GTTCATTTAAAAGTGATGTG+AGG	0.641856	2.2:+54582417	MS.gene65284:CDS
GGTAACGAAGTTAATCCAGT+TGG	0.642015	2.2:+54582248	MS.gene65284:CDS
GTTGCAAATCCACCATCTGA+AGG	0.643788	2.2:-54582701	None:intergenic
TTAAATGAACCTTTAGACGG+CGG	0.669154	2.2:-54582404	None:intergenic
TTAAAGGTGGGACACCAATG+AGG	0.687393	2.2:+54582771	MS.gene65284:CDS
TGTCAAAATCAAATACATCG+CGG	0.787139	2.2:+54582223	MS.gene65284:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TACTTTTTCTGTTTATTATT+AGG	-	chr2.2:54582884-54582903	None:intergenic	15.0%
!!!	TTTATCTTGATAATTTGATT+AGG	+	chr2.2:54582744-54582763	MS.gene65284:CDS	15.0%
!!	AAATTTATTTGATGCTTTGT+TGG	+	chr2.2:54582607-54582626	MS.gene65284:CDS	20.0%
!!	CTATTGAGACTTATATATTT+GGG	+	chr2.2:54582801-54582820	MS.gene65284:CDS	20.0%
!!	GAGTAAATATTTGCAAATAA+AGG	-	chr2.2:54582488-54582507	None:intergenic	20.0%
!!!	ACAAAGCATCAAATAAATTT+TGG	-	chr2.2:54582607-54582626	None:intergenic	20.0%
!!!	TGATATATATTTTGAATTGC+CGG	-	chr2.2:54582305-54582324	None:intergenic	20.0%
!	AATTTGATTAGGCATGTTAA+AGG	+	chr2.2:54582755-54582774	MS.gene65284:CDS	25.0%
!	CATATTTGGATCCAATTATA+GGG	+	chr2.2:54582441-54582460	MS.gene65284:CDS	25.0%
!	CCAAATATATAAGTCTCAAT+AGG	-	chr2.2:54582803-54582822	None:intergenic	25.0%
!	CCTATTGAGACTTATATATT+TGG	+	chr2.2:54582800-54582819	MS.gene65284:CDS	25.0%
!	TATACTAAGTACCCTATAAT+TGG	-	chr2.2:54582455-54582474	None:intergenic	25.0%
!	TCATATTTGGATCCAATTAT+AGG	+	chr2.2:54582440-54582459	MS.gene65284:CDS	25.0%
!!	CTTTTAAATGAACCTTTAGA+CGG	-	chr2.2:54582410-54582429	None:intergenic	25.0%
!!!	AAACAGAAAAAGTATCCATT+TGG	+	chr2.2:54582890-54582909	MS.gene65284:CDS	25.0%
!!!	ATGTATTTGATTTTGACACT+AGG	-	chr2.2:54582221-54582240	None:intergenic	25.0%
!!!	TGTATTTGATTTTGACACTA+GGG	-	chr2.2:54582220-54582239	None:intergenic	25.0%
	AAATCTTCACGATCAAATCA+AGG	+	chr2.2:54582340-54582359	MS.gene65284:CDS	30.0%
	ACAAAGACATTGCAAATCAA+TGG	+	chr2.2:54582168-54582187	MS.gene65284:CDS	30.0%
	CAAAGACATTGCAAATCAAT+GGG	+	chr2.2:54582169-54582188	MS.gene65284:CDS	30.0%
	GTTCATTTAAAAGTGATGTG+AGG	+	chr2.2:54582417-54582436	MS.gene65284:CDS	30.0%
	TGTCAAAATCAAATACATCG+CGG	+	chr2.2:54582223-54582242	MS.gene65284:CDS	30.0%
	TTCTCCAAATGTTGTAGTTA+GGG	+	chr2.2:54582565-54582584	MS.gene65284:CDS	30.0%
!	ATTCGCAAAATTTGTTCTTC+CGG	+	chr2.2:54582283-54582302	MS.gene65284:CDS	30.0%
!	ATTGAAGTGAGATGTCTTTT+GGG	-	chr2.2:54582532-54582551	None:intergenic	30.0%
!	CATATAATGTTTTCACAAGC+AGG	+	chr2.2:54581945-54581964	MS.gene65284:CDS	30.0%
!	GCAATTGATAATACTGGAAT+TGG	+	chr2.2:54582644-54582663	MS.gene65284:CDS	30.0%
!	TATTGAAGTGAGATGTCTTT+TGG	-	chr2.2:54582533-54582552	None:intergenic	30.0%
!!	TTGATTTGCAATGTCTTTGT+TGG	-	chr2.2:54582168-54582187	None:intergenic	30.0%
!!!	TACTGGAATTGGTTTTGTTA+AGG	+	chr2.2:54582655-54582674	MS.gene65284:CDS	30.0%
!!!	TAGCAATGTTTTGAAGATCT+GGG	-	chr2.2:54582145-54582164	None:intergenic	30.0%
	AAAATCAAATACATCGCGGT+CGG	+	chr2.2:54582227-54582246	MS.gene65284:CDS	35.0%
	AAGTGATGTGAGGTCATATT+TGG	+	chr2.2:54582427-54582446	MS.gene65284:CDS	35.0%
	CAAACAACATCCAAAGAATG+AGG	+	chr2.2:54582045-54582064	MS.gene65284:CDS	35.0%
	CTTCTCCAAATGTTGTAGTT+AGG	+	chr2.2:54582564-54582583	MS.gene65284:CDS	35.0%
	GAATAAGAAGAATCCTGAGT+TGG	+	chr2.2:54582835-54582854	MS.gene65284:CDS	35.0%
	GATCATATAGCCTCATTCTT+TGG	-	chr2.2:54582058-54582077	None:intergenic	35.0%
	TATCATGGTCATGTCAATAG+CGG	-	chr2.2:54582372-54582391	None:intergenic	35.0%
	TGATTAGGCATGTTAAAGGT+GGG	+	chr2.2:54582759-54582778	MS.gene65284:CDS	35.0%
	TTAAATGAACCTTTAGACGG+CGG	-	chr2.2:54582407-54582426	None:intergenic	35.0%
	TTGATTAGGCATGTTAAAGG+TGG	+	chr2.2:54582758-54582777	MS.gene65284:CDS	35.0%
!	CATGCTGCAATTGATAATAC+TGG	+	chr2.2:54582638-54582657	MS.gene65284:CDS	35.0%
!	GCTCAAATAGGTGTTTGTTA+TGG	+	chr2.2:54581969-54581988	MS.gene65284:CDS	35.0%
!!	AATAGGTGTTTGTTATGGCA+TGG	+	chr2.2:54581974-54581993	MS.gene65284:CDS	35.0%
!!!	ATGTTTTGAAGATCTGGGTT+AGG	-	chr2.2:54582140-54582159	None:intergenic	35.0%
!!!	GTAGCAATGTTTTGAAGATC+TGG	-	chr2.2:54582146-54582165	None:intergenic	35.0%
	AACGAAGTTAATCCAGTTGG+TGG	+	chr2.2:54582251-54582270	MS.gene65284:CDS	40.0%
	AAGGTTGTTGTGTCTGAAAG+TGG	+	chr2.2:54582674-54582693	MS.gene65284:CDS	40.0%
	AATTGAGAATTGCCACCAAC+TGG	-	chr2.2:54582266-54582285	None:intergenic	40.0%
	CCAAAATGTTTCTCCAACTC+AGG	-	chr2.2:54582851-54582870	None:intergenic	40.0%
	CCAAATGTTGTAGTTAGGGA+TGG	+	chr2.2:54582569-54582588	MS.gene65284:CDS	40.0%
	CCATCCCTAACTACAACATT+TGG	-	chr2.2:54582572-54582591	None:intergenic	40.0%
	CGGATACGAAGTTTCTATCA+TGG	-	chr2.2:54582387-54582406	None:intergenic	40.0%
	GGTAACGAAGTTAATCCAGT+TGG	+	chr2.2:54582248-54582267	MS.gene65284:CDS	40.0%
	TCTATTGCTTCACGTTGTGA+TGG	-	chr2.2:54582014-54582033	None:intergenic	40.0%
	TCTGGCATAGAGCTTATGTT+AGG	+	chr2.2:54582110-54582129	MS.gene65284:CDS	40.0%
	TGTAGTTAGGGATGGTTCAA+GGG	+	chr2.2:54582577-54582596	MS.gene65284:CDS	40.0%
	TTCACGTTGTGATGGTAGAT+TGG	-	chr2.2:54582006-54582025	None:intergenic	40.0%
	TTGTAGTTAGGGATGGTTCA+AGG	+	chr2.2:54582576-54582595	MS.gene65284:CDS	40.0%
	TTGTTGTGTCTGAAAGTGGT+TGG	+	chr2.2:54582678-54582697	MS.gene65284:CDS	40.0%
!	CTTGAAGCACTTAGAAACTC+TGG	+	chr2.2:54582092-54582111	MS.gene65284:CDS	40.0%
!!	CCTGAGTTGGAGAAACATTT+TGG	+	chr2.2:54582848-54582867	MS.gene65284:CDS	40.0%
!!	TAAGTGCTTCAAGAGCATCT+TGG	-	chr2.2:54582085-54582104	None:intergenic	40.0%
	GTAGTTAGGGATGGTTCAAG+GGG	+	chr2.2:54582578-54582597	MS.gene65284:CDS	45.0%
	GTTGCAAATCCACCATCTGA+AGG	-	chr2.2:54582704-54582723	None:intergenic	45.0%
	TCAATAGGTCCAGACCTCAT+TGG	-	chr2.2:54582788-54582807	None:intergenic	45.0%
	TCACAAGCAGGTGCTCAAAT+AGG	+	chr2.2:54581957-54581976	MS.gene65284:CDS	45.0%
	TTAAAGGTGGGACACCAATG+AGG	+	chr2.2:54582771-54582790	MS.gene65284:CDS	45.0%
!!	GCTTCAAGAGCATCTTGGTT+TGG	-	chr2.2:54582080-54582099	None:intergenic	45.0%
!!	GGTGTTTGTTATGGCATGGA+AGG	+	chr2.2:54581978-54581997	MS.gene65284:CDS	45.0%
	ACTTCGTATCCGCCGTCTAA+AGG	+	chr2.2:54582395-54582414	MS.gene65284:CDS	50.0%
!!	GAAAGTGGTTGGCCTTCAGA+TGG	+	chr2.2:54582689-54582708	MS.gene65284:CDS	50.0%
!!	AGTGGTTGGCCTTCAGATGG+TGG	+	chr2.2:54582692-54582711	MS.gene65284:CDS	55.0%
	GGTGGGACACCAATGAGGTC+TGG	+	chr2.2:54582776-54582795	MS.gene65284:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	54581942	54582919	54581942	ID=MS.gene65284
chr2.2	mRNA	54581942	54582919	54581942	ID=MS.gene65284.t1;Parent=MS.gene65284
chr2.2	exon	54581942	54582919	54581942	ID=MS.gene65284.t1.exon1;Parent=MS.gene65284.t1
chr2.2	CDS	54581942	54582919	54581942	ID=cds.MS.gene65284.t1;Parent=MS.gene65284.t1

Gene Sequence

>MS.gene65284

ATGCATATAATGTTTTCACAAGCAGGTGCTCAAATAGGTGTTTGTTATGGCATGGAAGGAACCAATCTACCATCACAACGTGAAGCAATAGATCTTTGCAAATCAAACAACATCCAAAGAATGAGGCTATATGATCCAAACCAAGATGCTCTTGAAGCACTTAGAAACTCTGGCATAGAGCTTATGTTAGGTGTGCCTAACCCAGATCTTCAAAACATTGCTACCAACAAAGACATTGCAAATCAATGGGTACAAAAAAATGTACTAAACTTCTACCCTAGTGTCAAAATCAAATACATCGCGGTCGGTAACGAAGTTAATCCAGTTGGTGGCAATTCTCAATTCGCAAAATTTGTTCTTCCGGCAATTCAAAATATATATCAAGCAATAAGAGCGAAAAATCTTCACGATCAAATCAAGGTTTCAACCGCTATTGACATGACCATGATAGAAACTTCGTATCCGCCGTCTAAAGGTTCATTTAAAAGTGATGTGAGGTCATATTTGGATCCAATTATAGGGTACTTAGTATATGCAAATGCACCTTTATTTGCAAATATTTACTCTTATTTTAGCTATAAAGATAACCCAAAAGACATCTCACTTCAATATGCTTTATTCACTTCTCCAAATGTTGTAGTTAGGGATGGTTCAAGGGGATACCAAAATTTATTTGATGCTTTGTTGGATTCATTACATGCTGCAATTGATAATACTGGAATTGGTTTTGTTAAGGTTGTTGTGTCTGAAAGTGGTTGGCCTTCAGATGGTGGATTTGCAACTACTTATGATAATGCAAGAATTTATCTTGATAATTTGATTAGGCATGTTAAAGGTGGGACACCAATGAGGTCTGGACCTATTGAGACTTATATATTTGGGTTGTTTGATGAGAATAAGAAGAATCCTGAGTTGGAGAAACATTTTGGAGTATTTAATCCTAATAATAAACAGAAAAAGTATCCATTTGGATTTTAG

Protein sequence

>MS.gene65284.t1

MHIMFSQAGAQIGVCYGMEGTNLPSQREAIDLCKSNNIQRMRLYDPNQDALEALRNSGIELMLGVPNPDLQNIATNKDIANQWVQKNVLNFYPSVKIKYIAVGNEVNPVGGNSQFAKFVLPAIQNIYQAIRAKNLHDQIKVSTAIDMTMIETSYPPSKGSFKSDVRSYLDPIIGYLVYANAPLFANIYSYFSYKDNPKDISLQYALFTSPNVVVRDGSRGYQNLFDALLDSLHAAIDNTGIGFVKVVVSESGWPSDGGFATTYDNARIYLDNLIRHVKGGTPMRSGPIETYIFGLFDENKKNPELEKHFGVFNPNNKQKKYPFGF