Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65418.t1 | KEH28561.1 | 85.7 | 119 | 14 | 1 | 1 | 116 | 1 | 119 | 1.60E-44 | 189.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65418.t1 | A0A072UGJ1 | 85.7 | 119 | 14 | 1 | 1 | 116 | 1 | 119 | 1.1e-44 | 189.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene65418 | 0.844411 | 7.69E-59 | -1.69E-46 |
| MS.gene050301 | MS.gene65418 | 0.82173 | 3.46E-53 | -1.69E-46 |
| MS.gene050900 | MS.gene65418 | 0.801702 | 7.95E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene65418 | 0.834268 | 3.31E-56 | -1.69E-46 |
| MS.gene053184 | MS.gene65418 | 0.847966 | 8.28E-60 | -1.69E-46 |
| MS.gene053836 | MS.gene65418 | 0.828588 | 8.27E-55 | -1.69E-46 |
| MS.gene054473 | MS.gene65418 | 0.820943 | 5.25E-53 | -1.69E-46 |
| MS.gene054542 | MS.gene65418 | 0.875315 | 3.39E-68 | -1.69E-46 |
| MS.gene057450 | MS.gene65418 | 0.802448 | 5.58E-49 | -1.69E-46 |
| MS.gene057782 | MS.gene65418 | 0.800728 | 1.26E-48 | -1.69E-46 |
| MS.gene058002 | MS.gene65418 | 0.801266 | 9.77E-49 | -1.69E-46 |
| MS.gene05811 | MS.gene65418 | 0.827597 | 1.43E-54 | -1.69E-46 |
| MS.gene058236 | MS.gene65418 | 0.800094 | 1.69E-48 | -1.69E-46 |
| MS.gene05842 | MS.gene65418 | 0.814679 | 1.36E-51 | -1.69E-46 |
| MS.gene058845 | MS.gene65418 | 0.806296 | 8.81E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene65418 | 0.836884 | 7.20E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene65418 | 0.810522 | 1.11E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene65418 | 0.807891 | 4.05E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene65418 | 0.831674 | 1.46E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65418.t1 | MTR_4g007550 | 85.714 | 119 | 14 | 1 | 1 | 116 | 1 | 119 | 2.27e-62 | 188 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTAAGATGTAGGAAACTA+TGG | 0.353441 | 4.3:+87903110 | MS.gene65418:intron |
| GCTCTACATCCAAGTTATCT+TGG | 0.366777 | 4.3:+87903288 | MS.gene65418:CDS |
| AGACCTAACACGGTTCGAAT+AGG | 0.402827 | 4.3:-87903606 | None:intergenic |
| ATTAAGAAAGCTAGAGGAAA+AGG | 0.431070 | 4.3:+87903215 | MS.gene65418:CDS |
| GACCTAACACGGTTCGAATA+GGG | 0.446620 | 4.3:-87903605 | None:intergenic |
| AGCCCTATTCGAACCGTGTT+AGG | 0.457843 | 4.3:+87903603 | MS.gene65418:CDS |
| GCAAGGGGAGAGAGTGAATA+CGG | 0.507712 | 4.3:+87903629 | MS.gene65418:CDS |
| GCTAGAGGAAAAGGAGCTAA+AGG | 0.517100 | 4.3:+87903224 | MS.gene65418:CDS |
| GAAGAATGCTTACTACTAGT+AGG | 0.521612 | 4.3:-87903002 | None:intergenic |
| AGGTGAAATCCAAGATAACT+TGG | 0.531170 | 4.3:-87903297 | None:intergenic |
| AACTATGGAAGCATAAAAGA+AGG | 0.547768 | 4.3:+87903125 | MS.gene65418:CDS |
| AGAAGATAAGAAACATATCA+AGG | 0.562331 | 4.3:+87903163 | MS.gene65418:CDS |
| CTCCCCTTGCAGACCTAACA+CGG | 0.568523 | 4.3:-87903616 | None:intergenic |
| ATTTCCAAATCCAATCAGAA+AGG | 0.583110 | 4.3:+87903537 | MS.gene65418:CDS |
| GAACCGTGTTAGGTCTGCAA+GGG | 0.596457 | 4.3:+87903613 | MS.gene65418:CDS |
| GACTCGATTAAGAAAGCTAG+AGG | 0.596569 | 4.3:+87903209 | MS.gene65418:CDS |
| AAACTCGTCAAATAGCATCA+TGG | 0.602558 | 4.3:+87902968 | MS.gene65418:CDS |
| ACGGTTCGAATAGGGCTACT+CGG | 0.617568 | 4.3:-87903597 | None:intergenic |
| CGAACCGTGTTAGGTCTGCA+AGG | 0.628387 | 4.3:+87903612 | MS.gene65418:CDS |
| AAAACAAAGCTTACACACAC+AGG | 0.629592 | 4.3:-87903317 | None:intergenic |
| AAAGAAGGAAGCAAGTTGCA+CGG | 0.651204 | 4.3:+87903140 | MS.gene65418:CDS |
| AACCGTGTTAGGTCTGCAAG+GGG | 0.658848 | 4.3:+87903614 | MS.gene65418:CDS |
| AGAGGAAAAGGAGCTAAAGG+TGG | 0.683202 | 4.3:+87903227 | MS.gene65418:CDS |
| AGATAAGAAACATATCAAGG+AGG | 0.727302 | 4.3:+87903166 | MS.gene65418:CDS |
| GTAGAGCTTCGAGAACGACG+AGG | 0.744495 | 4.3:-87903272 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTCAAGTTATGAGAAATT+AGG | - | chr4.3:87903472-87903491 | None:intergenic | 20.0% |
| ! | AGAAGATAAGAAACATATCA+AGG | + | chr4.3:87903163-87903182 | MS.gene65418:CDS | 25.0% |
| ! | TATAGCATGAGTACAATTTA+AGG | - | chr4.3:87903048-87903067 | None:intergenic | 25.0% |
| ! | TATTAAGATGTAGGAAACTA+TGG | + | chr4.3:87903110-87903129 | MS.gene65418:intron | 25.0% |
| ! | TGCTTATTCTATTAAGATGT+AGG | + | chr4.3:87903101-87903120 | MS.gene65418:intron | 25.0% |
| AGATAAGAAACATATCAAGG+AGG | + | chr4.3:87903166-87903185 | MS.gene65418:CDS | 30.0% | |
| ATTAAGAAAGCTAGAGGAAA+AGG | + | chr4.3:87903215-87903234 | MS.gene65418:CDS | 30.0% | |
| ATTTCCAAATCCAATCAGAA+AGG | + | chr4.3:87903537-87903556 | MS.gene65418:CDS | 30.0% | |
| TCATAGCAACTTAACATGAA+AGG | - | chr4.3:87903357-87903376 | None:intergenic | 30.0% | |
| ! | AACTATGGAAGCATAAAAGA+AGG | + | chr4.3:87903125-87903144 | MS.gene65418:CDS | 30.0% |
| ! | ACATGTTTATGTTTGAAGCT+TGG | + | chr4.3:87903397-87903416 | MS.gene65418:intron | 30.0% |
| ! | CATGTTTATGTTTGAAGCTT+GGG | + | chr4.3:87903398-87903417 | MS.gene65418:intron | 30.0% |
| ! | TTTTCCTTTCTGATTGGATT+TGG | - | chr4.3:87903544-87903563 | None:intergenic | 30.0% |
| !!! | ACTCAAGCTATATCGATTTT+AGG | + | chr4.3:87902921-87902940 | MS.gene65418:CDS | 30.0% |
| AAAACAAAGCTTACACACAC+AGG | - | chr4.3:87903320-87903339 | None:intergenic | 35.0% | |
| AAACTCGTCAAATAGCATCA+TGG | + | chr4.3:87902968-87902987 | MS.gene65418:CDS | 35.0% | |
| AGGTGAAATCCAAGATAACT+TGG | - | chr4.3:87903300-87903319 | None:intergenic | 35.0% | |
| GAAGAATGCTTACTACTAGT+AGG | - | chr4.3:87903005-87903024 | None:intergenic | 35.0% | |
| ! | CGATGATTTTCCTTTCTGAT+TGG | - | chr4.3:87903550-87903569 | None:intergenic | 35.0% |
| AAAGAAGGAAGCAAGTTGCA+CGG | + | chr4.3:87903140-87903159 | MS.gene65418:CDS | 40.0% | |
| AAGGAAACGCTCCTTCAAAA+AGG | - | chr4.3:87903029-87903048 | None:intergenic | 40.0% | |
| GACTCGATTAAGAAAGCTAG+AGG | + | chr4.3:87903209-87903228 | MS.gene65418:CDS | 40.0% | |
| GCTCTACATCCAAGTTATCT+TGG | + | chr4.3:87903288-87903307 | MS.gene65418:CDS | 40.0% | |
| ! | AGCATTCTTCGCCTTTTTGA+AGG | + | chr4.3:87903015-87903034 | MS.gene65418:intron | 40.0% |
| AGACCTAACACGGTTCGAAT+AGG | - | chr4.3:87903609-87903628 | None:intergenic | 45.0% | |
| AGAGGAAAAGGAGCTAAAGG+TGG | + | chr4.3:87903227-87903246 | MS.gene65418:CDS | 45.0% | |
| GACCTAACACGGTTCGAATA+GGG | - | chr4.3:87903608-87903627 | None:intergenic | 45.0% | |
| GCTAGAGGAAAAGGAGCTAA+AGG | + | chr4.3:87903224-87903243 | MS.gene65418:CDS | 45.0% | |
| AACCGTGTTAGGTCTGCAAG+GGG | + | chr4.3:87903614-87903633 | MS.gene65418:CDS | 50.0% | |
| ACGGTTCGAATAGGGCTACT+CGG | - | chr4.3:87903600-87903619 | None:intergenic | 50.0% | |
| AGCCCTATTCGAACCGTGTT+AGG | + | chr4.3:87903603-87903622 | MS.gene65418:CDS | 50.0% | |
| GAACCGTGTTAGGTCTGCAA+GGG | + | chr4.3:87903613-87903632 | MS.gene65418:CDS | 50.0% | |
| GCAAGGGGAGAGAGTGAATA+CGG | + | chr4.3:87903629-87903648 | MS.gene65418:CDS | 50.0% | |
| CGAACCGTGTTAGGTCTGCA+AGG | + | chr4.3:87903612-87903631 | MS.gene65418:CDS | 55.0% | |
| CTCCCCTTGCAGACCTAACA+CGG | - | chr4.3:87903619-87903638 | None:intergenic | 55.0% | |
| GTAGAGCTTCGAGAACGACG+AGG | - | chr4.3:87903275-87903294 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 87902879 | 87903674 | 87902879 | ID=MS.gene65418 |
| chr4.3 | mRNA | 87902879 | 87903674 | 87902879 | ID=MS.gene65418.t1;Parent=MS.gene65418 |
| chr4.3 | exon | 87902879 | 87903011 | 87902879 | ID=MS.gene65418.t1.exon1;Parent=MS.gene65418.t1 |
| chr4.3 | CDS | 87902879 | 87903011 | 87902879 | ID=cds.MS.gene65418.t1;Parent=MS.gene65418.t1 |
| chr4.3 | exon | 87903117 | 87903325 | 87903117 | ID=MS.gene65418.t1.exon2;Parent=MS.gene65418.t1 |
| chr4.3 | CDS | 87903117 | 87903325 | 87903117 | ID=cds.MS.gene65418.t1;Parent=MS.gene65418.t1 |
| chr4.3 | exon | 87903537 | 87903674 | 87903537 | ID=MS.gene65418.t1.exon3;Parent=MS.gene65418.t1 |
| chr4.3 | CDS | 87903537 | 87903674 | 87903537 | ID=cds.MS.gene65418.t1;Parent=MS.gene65418.t1 |
| Gene Sequence |
| Protein sequence |
