AlfalfaGEDB | MS.gene65481

M. sativa cultivar XinJiangDaYe / MS.gene65481

KEGG: -
GO: -
PFAM: PF04852.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65481.t1	XP_013467094.1	93.7	189	11	1	1	188	1	189	7.10E-69	270.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65481.t1	Q9LW68	89.6	125	13	0	64	188	48	172	4.2e-62	239.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65481.t1	A0A072VGF5	93.7	189	11	1	1	188	1	189	5.1e-69	270.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene65481	0.802314	5.95E-49	-1.69E-46
MS.gene050563	MS.gene65481	0.907257	6.16E-81	-1.69E-46
MS.gene050584	MS.gene65481	0.825273	5.13E-54	-1.69E-46
MS.gene050585	MS.gene65481	0.832689	8.19E-56	-1.69E-46
MS.gene050587	MS.gene65481	0.824256	8.91E-54	-1.69E-46
MS.gene05143	MS.gene65481	0.83253	8.97E-56	-1.69E-46
MS.gene051789	MS.gene65481	0.826646	2.42E-54	-1.69E-46
MS.gene052142	MS.gene65481	0.865951	4.07E-65	-1.69E-46
MS.gene052187	MS.gene65481	0.805858	1.09E-49	-1.69E-46
MS.gene052698	MS.gene65481	0.815119	1.09E-51	-1.69E-46
MS.gene053074	MS.gene65481	0.800663	1.30E-48	-1.69E-46
MS.gene053383	MS.gene65481	0.919991	2.25E-87	-1.69E-46
MS.gene05456	MS.gene65481	0.86766	1.16E-65	-1.69E-46
MS.gene055359	MS.gene65481	0.858656	7.09E-63	-1.69E-46
MS.gene058918	MS.gene65481	0.829759	4.30E-55	-1.69E-46
MS.gene059006	MS.gene65481	0.834579	2.76E-56	-1.69E-46
MS.gene059632	MS.gene65481	0.807811	4.21E-50	-1.69E-46
MS.gene059768	MS.gene65481	0.826456	2.69E-54	-1.69E-46
MS.gene060691	MS.gene65481	0.819277	1.26E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65481.t1	MTR_1g442860	97.664	214	4	1	1	213	1	214	3.92e-154	426
MS.gene65481.t1	MTR_1g075990	79.679	187	33	1	19	205	13	194	1.48e-91	267
MS.gene65481.t1	MTR_5g072510	80.282	142	28	0	54	195	27	168	9.57e-88	258
MS.gene65481.t1	MTR_4g094428	83.704	135	22	0	63	197	45	179	9.55e-87	256
MS.gene65481.t1	MTR_7g097030	73.711	194	51	0	1	194	1	194	8.06e-86	253
MS.gene65481.t1	MTR_1g069825	74.227	194	38	1	1	194	1	182	1.85e-84	249
MS.gene65481.t1	MTR_3g031830	71.345	171	37	2	37	195	3	173	1.99e-76	229
MS.gene65481.t1	MTR_2g092950	71.724	145	41	0	43	187	2	146	3.91e-75	224
MS.gene65481.t1	MTR_4g079930	74.436	133	34	0	55	187	20	152	1.23e-74	223
MS.gene65481.t1	MTR_2g076400	77.273	132	30	0	63	194	32	163	4.86e-73	219
MS.gene65481.t1	MTR_2g016110	72.662	139	38	0	56	194	27	165	9.10e-72	216
MS.gene65481.t1	MTR_7g115700	63.804	163	53	3	34	195	1	158	9.32e-71	213
MS.gene65481.t1	MTR_1g080210	84.000	125	20	0	64	188	40	164	2.36e-70	213
MS.gene65481.t1	MTR_8g086350	72.800	125	34	0	63	187	30	154	9.47e-58	181
MS.gene65481.t1	MTR_1g114020	78.431	51	11	0	57	107	64	114	7.77e-23	91.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65481.t1	AT1G07090	77.181	149	29	1	48	196	20	163	6.04e-87	255
MS.gene65481.t1	AT2G31160	84.277	159	24	1	38	195	27	185	1.59e-86	255
MS.gene65481.t1	AT3G23290	95.238	126	6	0	63	188	47	172	6.34e-82	242
MS.gene65481.t1	AT5G58500	80.741	135	25	1	62	195	17	151	9.43e-82	241
MS.gene65481.t1	AT5G28490	76.250	160	32	1	34	193	1	154	7.56e-76	227
MS.gene65481.t1	AT2G42610	68.182	154	43	1	34	187	1	148	6.27e-73	219
MS.gene65481.t1	AT2G42610	68.182	154	43	1	34	187	1	148	6.27e-73	219
MS.gene65481.t1	AT3G04510	69.753	162	49	0	32	193	1	162	1.13e-71	216
MS.gene65481.t1	AT1G78815	62.585	147	55	0	63	209	39	185	1.05e-66	204
MS.gene65481.t1	AT4G18610	69.841	126	37	1	63	187	36	161	8.13e-64	196
MS.gene65481.t1	AT1G16910	64.394	132	47	0	61	192	20	151	9.02e-62	190

Find 48 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCGTTACCTCGACCAATT+TGG	0.269006	1.1:+26500604	MS.gene65481:CDS
TGGATTTGGATGTCCATAAA+AGG	0.277270	1.1:-26500656	None:intergenic
GGACATGGTGCAGGTGGATT+TGG	0.320133	1.1:-26500670	None:intergenic
GACTTTACCTTCGCGAAGTT+AGG	0.323181	1.1:+26500809	MS.gene65481:CDS
GGGTTTGTTATGTTGATCAT+AGG	0.347871	1.1:-26500364	None:intergenic
AGAAGAGATTGGAACACATT+TGG	0.367128	1.1:+26500511	MS.gene65481:CDS
GTGGTGGTCTATGGTTTCTT+AGG	0.368488	1.1:-26500540	None:intergenic
GGTGGTGGTGGTGGCGGCTG+AGG	0.370057	1.1:-26500889	None:intergenic
GTCGTTGTTGATGTTGTTGA+TGG	0.375417	1.1:-26500463	None:intergenic
AAATTCTTGAAGAGAATTCA+TGG	0.377394	1.1:-26500302	None:intergenic
ACTTTACCTTCGCGAAGTTA+GGG	0.398859	1.1:+26500810	MS.gene65481:CDS
GTTGTAGTTGCATTATTTGA+TGG	0.419514	1.1:-26500910	None:intergenic
GCACCGAAAGGATTAGCTTC+TGG	0.420510	1.1:-26500775	None:intergenic
GGTTTGTTATGTTGATCATA+GGG	0.430093	1.1:-26500363	None:intergenic
GGTAGTCTTGATGCACTCAT+TGG	0.434097	1.1:+26500715	MS.gene65481:CDS
AGAATCAAAAGAGAAGAGAT+TGG	0.459394	1.1:+26500500	MS.gene65481:CDS
CTTGTCCTCTTCGCCAAGCG+TGG	0.459831	1.1:+26500692	MS.gene65481:CDS
AAGCCAGAAGCTAATCCTTT+CGG	0.463060	1.1:+26500772	MS.gene65481:CDS
GGTGGTGGTGGCGGCTGAGG+CGG	0.465046	1.1:-26500886	None:intergenic
GCATTATTTGATGGTGGTGG+TGG	0.471088	1.1:-26500901	None:intergenic
TTATTTGATGGTGGTGGTGG+TGG	0.487231	1.1:-26500898	None:intergenic
TTGTCCTCTTCGCCAAGCGT+GGG	0.488923	1.1:+26500693	MS.gene65481:CDS
CGAGGTAACGCAAGAATTCA+AGG	0.492274	1.1:-26500594	None:intergenic
AGAGTGAAAGTGGTGGTCTA+TGG	0.493036	1.1:-26500549	None:intergenic
GTCATCGTCATCGATGATGA+TGG	0.500415	1.1:-26500385	None:intergenic
GTAGTCTTGATGCACTCATT+GGG	0.507513	1.1:+26500716	MS.gene65481:CDS
GTTGCATTATTTGATGGTGG+TGG	0.513644	1.1:-26500904	None:intergenic
CACGCTTGGCGAAGAGGACA+AGG	0.527749	1.1:-26500691	None:intergenic
CTTTCACTCTCACGTTGTAG+TGG	0.531680	1.1:+26500562	MS.gene65481:CDS
GGATTTGGATGTCCATAAAA+GGG	0.532805	1.1:-26500655	None:intergenic
TGATGGTGGTGATGTTGTTG+TGG	0.537367	1.1:-26500446	None:intergenic
TGGTGATGTTGTTGTGGACG+AGG	0.542069	1.1:-26500440	None:intergenic
CATCAAGACTACCCCACGCT+TGG	0.543485	1.1:-26500705	None:intergenic
GTAGTTGCATTATTTGATGG+TGG	0.544780	1.1:-26500907	None:intergenic
GTTGTTGATGTTGTTGATGG+TGG	0.556628	1.1:-26500460	None:intergenic
AGAGGACAAGGACATGGTGC+AGG	0.565916	1.1:-26500679	None:intergenic
CTACCCCACGCTTGGCGAAG+AGG	0.567363	1.1:-26500697	None:intergenic
CTACAACGTGAGAGTGAAAG+TGG	0.580478	1.1:-26500559	None:intergenic
GGACAAGGACATGGTGCAGG+TGG	0.588264	1.1:-26500676	None:intergenic
TCATCGTCATCGATGATGAT+GGG	0.604263	1.1:-26500384	None:intergenic
TGTCCTCTTCGCCAAGCGTG+GGG	0.612011	1.1:+26500694	MS.gene65481:CDS
CGAACAGCACGAGCACCGAA+AGG	0.613288	1.1:-26500787	None:intergenic
TGTTGTAGATGACGATGGTA+TGG	0.613461	1.1:-26500407	None:intergenic
TGGCGAAGAGGACAAGGACA+TGG	0.629659	1.1:-26500685	None:intergenic
GATTCTCAAGCTAAAGCTAG+AGG	0.638819	1.1:+26500832	MS.gene65481:CDS
GAGAATCCCTAACTTCGCGA+AGG	0.649287	1.1:-26500816	None:intergenic
GCTGATGTTGTAGATGACGA+TGG	0.727039	1.1:-26500412	None:intergenic
CAACGTGAGAGTGAAAGTGG+TGG	0.746060	1.1:-26500556	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGAATCAAAAGAGAAGAGAT+TGG	+	chr1.1:26500500-26500519	MS.gene65481:CDS	30.0%
	GTTGTAGTTGCATTATTTGA+TGG	-	chr1.1:26500913-26500932	None:intergenic	30.0%
!!	GGTTTGTTATGTTGATCATA+GGG	-	chr1.1:26500366-26500385	None:intergenic	30.0%
!!	TTTTGATTCTCGTATCTACT+TGG	-	chr1.1:26500490-26500509	None:intergenic	30.0%
!!!	GAACCTTTGTTTTTCCAAAT+TGG	-	chr1.1:26500621-26500640	None:intergenic	30.0%
!!!	GTTCATCATGTCTTTGTTTT+TGG	-	chr1.1:26500338-26500357	None:intergenic	30.0%
	AGAAGAGATTGGAACACATT+TGG	+	chr1.1:26500511-26500530	MS.gene65481:CDS	35.0%
	CGACCAATTTGGAAAAACAA+AGG	+	chr1.1:26500615-26500634	MS.gene65481:CDS	35.0%
	GGATTTGGATGTCCATAAAA+GGG	-	chr1.1:26500658-26500677	None:intergenic	35.0%
	GTAGTTGCATTATTTGATGG+TGG	-	chr1.1:26500910-26500929	None:intergenic	35.0%
	TGGATTTGGATGTCCATAAA+AGG	-	chr1.1:26500659-26500678	None:intergenic	35.0%
!	ACTCAGATTTGTCCCTTTTA+TGG	+	chr1.1:26500643-26500662	MS.gene65481:CDS	35.0%
!!	GGGTTTGTTATGTTGATCAT+AGG	-	chr1.1:26500367-26500386	None:intergenic	35.0%
!!	TTGTTTTTCCAAATTGGTCG+AGG	-	chr1.1:26500615-26500634	None:intergenic	35.0%
	AAGCCAGAAGCTAATCCTTT+CGG	+	chr1.1:26500772-26500791	MS.gene65481:CDS	40.0%
	ACTTTACCTTCGCGAAGTTA+GGG	+	chr1.1:26500810-26500829	MS.gene65481:CDS	40.0%
	GATTCTCAAGCTAAAGCTAG+AGG	+	chr1.1:26500832-26500851	MS.gene65481:CDS	40.0%
	GTCGTTGTTGATGTTGTTGA+TGG	-	chr1.1:26500466-26500485	None:intergenic	40.0%
	TCATCGTCATCGATGATGAT+GGG	-	chr1.1:26500387-26500406	None:intergenic	40.0%
	TGTTGTAGATGACGATGGTA+TGG	-	chr1.1:26500410-26500429	None:intergenic	40.0%
!	GTAGTCTTGATGCACTCATT+GGG	+	chr1.1:26500716-26500735	MS.gene65481:CDS	40.0%
!	GTTGTTGATGTTGTTGATGG+TGG	-	chr1.1:26500463-26500482	None:intergenic	40.0%
!!	CTGCTTTTGAAGAGAATGGT+GGG	+	chr1.1:26500749-26500768	MS.gene65481:CDS	40.0%
!!	GTTGCATTATTTGATGGTGG+TGG	-	chr1.1:26500907-26500926	None:intergenic	40.0%
!!!	AGAGCTGCTTTTGAAGAGAA+TGG	+	chr1.1:26500745-26500764	MS.gene65481:CDS	40.0%
	AGAGTGAAAGTGGTGGTCTA+TGG	-	chr1.1:26500552-26500571	None:intergenic	45.0%
	CGAGGTAACGCAAGAATTCA+AGG	-	chr1.1:26500597-26500616	None:intergenic	45.0%
	CTACAACGTGAGAGTGAAAG+TGG	-	chr1.1:26500562-26500581	None:intergenic	45.0%
	CTTTCACTCTCACGTTGTAG+TGG	+	chr1.1:26500562-26500581	MS.gene65481:CDS	45.0%
	GACTTTACCTTCGCGAAGTT+AGG	+	chr1.1:26500809-26500828	MS.gene65481:CDS	45.0%
	GCTGATGTTGTAGATGACGA+TGG	-	chr1.1:26500415-26500434	None:intergenic	45.0%
	GTCATCGTCATCGATGATGA+TGG	-	chr1.1:26500388-26500407	None:intergenic	45.0%
	GTGGTGGTCTATGGTTTCTT+AGG	-	chr1.1:26500543-26500562	None:intergenic	45.0%
	TTGCGTTACCTCGACCAATT+TGG	+	chr1.1:26500604-26500623	MS.gene65481:CDS	45.0%
!	GGTAGTCTTGATGCACTCAT+TGG	+	chr1.1:26500715-26500734	MS.gene65481:CDS	45.0%
!!	GCATTATTTGATGGTGGTGG+TGG	-	chr1.1:26500904-26500923	None:intergenic	45.0%
!!	TGATGGTGGTGATGTTGTTG+TGG	-	chr1.1:26500449-26500468	None:intergenic	45.0%
!!	TTATTTGATGGTGGTGGTGG+TGG	-	chr1.1:26500901-26500920	None:intergenic	45.0%
!!!	GCTGCTTTTGAAGAGAATGG+TGG	+	chr1.1:26500748-26500767	MS.gene65481:CDS	45.0%
	CAACGTGAGAGTGAAAGTGG+TGG	-	chr1.1:26500559-26500578	None:intergenic	50.0%
	GAGAATCCCTAACTTCGCGA+AGG	-	chr1.1:26500819-26500838	None:intergenic	50.0%
!	TGGTGATGTTGTTGTGGACG+AGG	-	chr1.1:26500443-26500462	None:intergenic	50.0%
!!	GCACCGAAAGGATTAGCTTC+TGG	-	chr1.1:26500778-26500797	None:intergenic	50.0%
	AGAGGACAAGGACATGGTGC+AGG	-	chr1.1:26500682-26500701	None:intergenic	55.0%
	CATCAAGACTACCCCACGCT+TGG	-	chr1.1:26500708-26500727	None:intergenic	55.0%
	TGGCGAAGAGGACAAGGACA+TGG	-	chr1.1:26500688-26500707	None:intergenic	55.0%
	TTGTCCTCTTCGCCAAGCGT+GGG	+	chr1.1:26500693-26500712	MS.gene65481:CDS	55.0%
!	GGACATGGTGCAGGTGGATT+TGG	-	chr1.1:26500673-26500692	None:intergenic	55.0%
!!	TTTGATGGTGGTGGTGGTGG+CGG	-	chr1.1:26500898-26500917	None:intergenic	55.0%
	CACGCTTGGCGAAGAGGACA+AGG	-	chr1.1:26500694-26500713	None:intergenic	60.0%
	CTTGTCCTCTTCGCCAAGCG+TGG	+	chr1.1:26500692-26500711	MS.gene65481:CDS	60.0%
	GGACAAGGACATGGTGCAGG+TGG	-	chr1.1:26500679-26500698	None:intergenic	60.0%
	TGTCCTCTTCGCCAAGCGTG+GGG	+	chr1.1:26500694-26500713	MS.gene65481:CDS	60.0%
!!	CGAACAGCACGAGCACCGAA+AGG	-	chr1.1:26500790-26500809	None:intergenic	60.0%
	CTACCCCACGCTTGGCGAAG+AGG	-	chr1.1:26500700-26500719	None:intergenic	65.0%
!!	GGTGGTGGTGGCGGCTGAGG+CGG	-	chr1.1:26500889-26500908	None:intergenic	75.0%
!!	GGTGGTGGTGGTGGCGGCTG+AGG	-	chr1.1:26500892-26500911	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	26500304	26500945	26500304	ID=MS.gene65481
chr1.1	mRNA	26500304	26500945	26500304	ID=MS.gene65481.t1;Parent=MS.gene65481
chr1.1	exon	26500304	26500945	26500304	ID=MS.gene65481.t1.exon1;Parent=MS.gene65481.t1
chr1.1	CDS	26500304	26500945	26500304	ID=cds.MS.gene65481.t1;Parent=MS.gene65481.t1

Gene Sequence

>MS.gene65481

ATGAATTCTCTTCAAGAATTTGAATCATCAACCAAAAACAAAGACATGATGAACACAAACCCTATGATCAACATAACAAACCCATCATCATCGATGACGATGACCATACCATCGTCATCTACAACATCAGCATCATCCTCGTCCACAACAACATCACCACCATCAACAACATCAACAACGACACCAAGTAGATACGAGAATCAAAAGAGAAGAGATTGGAACACATTTGGACAATACCTAAGAAACCATAGACCACCACTTTCACTCTCACGTTGTAGTGGAGCTCATGTCCTTGAATTCTTGCGTTACCTCGACCAATTTGGAAAAACAAAGGTTCACACTCAGATTTGTCCCTTTTATGGACATCCAAATCCACCTGCACCATGTCCTTGTCCTCTTCGCCAAGCGTGGGGTAGTCTTGATGCACTCATTGGGAGACTTAGAGCTGCTTTTGAAGAGAATGGTGGGAAGCCAGAAGCTAATCCTTTCGGTGCTCGTGCTGTTCGACTTTACCTTCGCGAAGTTAGGGATTCTCAAGCTAAAGCTAGAGGTATTAGCTATGAGAAAAAGAAGCGCAAGCGTCCGCCTCAGCCGCCACCACCACCACCATCAAATAATGCAACTACAACTGATCTTCACTAG

Protein sequence

>MS.gene65481.t1

MNSLQEFESSTKNKDMMNTNPMINITNPSSSMTMTIPSSSTTSASSSSTTTSPPSTTSTTTPSRYENQKRRDWNTFGQYLRNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTQICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPEANPFGARAVRLYLREVRDSQAKARGISYEKKKRKRPPQPPPPPPSNNATTTDLH