AlfalfaGEDB | MS.gene66845

M. sativa cultivar XinJiangDaYe / MS.gene66845

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66845.t1	XP_013465951.1	98.1	427	7	1	1	427	1	426	2.80E-221	777.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66845.t1	Q6NKN9	44.9	354	139	12	85	427	58	366	4.5e-55	216.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66845.t1	A0A072VPD3	98.1	427	7	1	1	427	1	426	2.0e-221	777.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene66845.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049350	MS.gene66845	0.805612	1.23E-49	-1.69E-46
MS.gene049711	MS.gene66845	0.80535	1.39E-49	-1.69E-46
MS.gene049807	MS.gene66845	0.849017	4.24E-60	-1.69E-46
MS.gene051234	MS.gene66845	0.812827	3.48E-51	-1.69E-46
MS.gene051450	MS.gene66845	0.820699	5.98E-53	-1.69E-46
MS.gene052609	MS.gene66845	0.801542	8.57E-49	-1.69E-46
MS.gene053143	MS.gene66845	0.837423	5.24E-57	-1.69E-46
MS.gene053144	MS.gene66845	0.820714	5.93E-53	-1.69E-46
MS.gene054554	MS.gene66845	0.801994	6.92E-49	-1.69E-46
MS.gene054584	MS.gene66845	0.825102	5.63E-54	-1.69E-46
MS.gene054585	MS.gene66845	0.810304	1.23E-50	-1.69E-46
MS.gene055221	MS.gene66845	0.810022	1.42E-50	-1.69E-46
MS.gene055469	MS.gene66845	0.806085	9.76E-50	-1.69E-46
MS.gene055537	MS.gene66845	0.813912	2.01E-51	-1.69E-46
MS.gene055975	MS.gene66845	0.813733	2.20E-51	-1.69E-46
MS.gene058149	MS.gene66845	0.804074	2.57E-49	-1.69E-46
MS.gene058492	MS.gene66845	0.854926	8.89E-62	-1.69E-46
MS.gene058668	MS.gene66845	0.833586	4.90E-56	-1.69E-46
MS.gene059778	MS.gene66845	0.815615	8.44E-52	-1.69E-46
MS.gene061100	MS.gene66845	0.818848	1.58E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66845.t1	MTR_1g017350	98.595	427	5	1	1	427	1	426	0.0	858
MS.gene66845.t1	MTR_1g017350	97.892	427	5	2	1	427	1	423	0.0	847
MS.gene66845.t1	MTR_1g017350	98.341	422	6	1	1	422	1	421	0.0	847
MS.gene66845.t1	MTR_1g017350	97.630	422	6	2	1	422	1	418	0.0	837
MS.gene66845.t1	MTR_5g048860	55.000	200	62	5	196	391	300	475	6.85e-56	194
MS.gene66845.t1	MTR_8g065740	41.768	328	136	10	93	391	114	415	1.57e-55	191
MS.gene66845.t1	MTR_3g116770	48.558	208	76	4	233	409	163	370	8.53e-51	176
MS.gene66845.t1	MTR_7g053410	39.752	322	152	8	75	355	91	411	1.01e-50	179
MS.gene66845.t1	MTR_7g053410	39.752	322	152	8	75	355	91	411	1.44e-50	179
MS.gene66845.t1	MTR_3g116770	46.890	209	79	4	233	409	163	371	1.85e-50	176
MS.gene66845.t1	MTR_8g012290	38.418	354	161	15	88	391	81	427	2.31e-47	169
MS.gene66845.t1	MTR_8g012290	38.418	354	161	15	88	391	81	427	2.58e-47	169
MS.gene66845.t1	MTR_4g070320	52.500	160	71	2	194	348	71	230	2.57e-44	157
MS.gene66845.t1	MTR_8g062240	47.475	198	96	4	171	360	53	250	1.31e-42	151
MS.gene66845.t1	MTR_3g498825	66.981	106	32	1	243	345	129	234	2.05e-40	147
MS.gene66845.t1	MTR_8g099880	73.626	91	21	1	258	345	142	232	8.33e-40	145
MS.gene66845.t1	MTR_7g092510	68.041	97	31	0	250	346	162	258	1.54e-39	145
MS.gene66845.t1	MTR_5g037250	60.484	124	44	2	209	332	65	183	1.64e-38	139
MS.gene66845.t1	MTR_1g059270	61.165	103	40	0	250	352	149	251	1.92e-38	139
MS.gene66845.t1	MTR_1g059270	63.918	97	35	0	250	346	149	245	4.54e-37	138
MS.gene66845.t1	MTR_6g084120	52.000	100	38	2	241	340	136	225	4.44e-24	100
MS.gene66845.t1	MTR_4g131160	60.241	83	32	1	244	326	179	260	2.74e-22	98.6
MS.gene66845.t1	MTR_4g131160	60.241	83	32	1	244	326	182	263	3.04e-22	98.2
MS.gene66845.t1	MTR_1g080890	67.164	67	21	1	260	326	192	257	7.81e-21	93.6
MS.gene66845.t1	MTR_4g033295	58.462	65	27	0	261	325	118	182	1.91e-16	77.8
MS.gene66845.t1	MTR_2g098310	47.126	87	38	1	247	325	310	396	2.55e-16	80.9
MS.gene66845.t1	MTR_4g033295	56.716	67	28	1	261	326	118	184	2.75e-16	77.0
MS.gene66845.t1	MTR_1g052470	52.055	73	35	0	259	331	296	368	5.17e-16	79.7
MS.gene66845.t1	MTR_7g092540	52.113	71	34	0	261	331	243	313	7.18e-16	78.6
MS.gene66845.t1	MTR_4g065870	56.716	67	29	0	259	325	353	419	7.22e-16	79.7
MS.gene66845.t1	MTR_2g098310	47.674	86	37	1	247	324	310	395	7.69e-16	79.3
MS.gene66845.t1	MTR_1g084980	47.945	73	37	1	263	335	464	535	1.40e-15	79.3
MS.gene66845.t1	MTR_7g092540	54.688	64	29	0	261	324	243	306	3.84e-15	76.3
MS.gene66845.t1	MTR_1g070870	51.579	95	45	1	232	326	91	184	4.14e-15	75.9
MS.gene66845.t1	MTR_4g065870	58.065	62	26	0	261	322	355	416	4.42e-15	77.0
MS.gene66845.t1	MTR_1g069155	52.174	69	32	1	263	331	319	386	6.14e-15	77.0
MS.gene66845.t1	MTR_1g070870	65.672	67	22	1	260	326	122	187	1.84e-14	73.9
MS.gene66845.t1	MTR_7g099540	40.952	105	58	2	230	331	218	321	2.54e-14	75.1
MS.gene66845.t1	MTR_3g449770	41.284	109	59	3	222	326	266	373	7.70e-14	73.6
MS.gene66845.t1	MTR_3g028520	47.761	67	35	0	259	325	320	386	7.30e-13	70.1
MS.gene66845.t1	MTR_3g027650	47.761	67	35	0	259	325	320	386	7.30e-13	70.1
MS.gene66845.t1	MTR_7g099540	40.000	100	56	2	230	326	218	316	9.36e-13	70.1
MS.gene66845.t1	MTR_7g110810	48.387	62	31	1	272	333	213	273	3.40e-12	67.8
MS.gene66845.t1	MTR_1g018090	50.769	65	31	1	261	325	274	337	3.80e-12	67.8
MS.gene66845.t1	MTR_3g027650	50.000	62	31	0	261	322	322	383	4.25e-12	67.8
MS.gene66845.t1	MTR_1g019240	46.154	65	34	1	263	327	87	150	6.88e-12	65.9
MS.gene66845.t1	MTR_8g036235	39.506	81	41	1	253	325	300	380	7.45e-12	67.0
MS.gene66845.t1	MTR_7g111320	53.125	64	29	1	263	326	434	496	1.71e-11	66.6
MS.gene66845.t1	MTR_3g103030	51.562	64	30	1	262	325	230	292	2.70e-11	64.7
MS.gene66845.t1	MTR_2g098310	33.884	121	37	2	247	324	310	430	9.16e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66845.t1	AT1G10120	42.664	443	161	21	1	427	1	366	3.84e-77	244
MS.gene66845.t1	AT1G10120	42.664	443	161	21	1	427	1	366	3.84e-77	244
MS.gene66845.t1	AT1G68920	63.871	155	30	3	238	392	285	413	4.69e-55	190
MS.gene66845.t1	AT1G68920	63.871	155	30	3	238	392	286	414	5.00e-55	190
MS.gene66845.t1	AT1G68920	63.871	155	30	3	238	392	286	414	5.00e-55	190
MS.gene66845.t1	AT1G68920	63.871	155	30	3	238	392	286	414	5.00e-55	190
MS.gene66845.t1	AT1G26260	69.231	130	34	2	226	350	133	261	9.16e-52	177
MS.gene66845.t1	AT1G26260	64.583	144	45	2	212	350	148	290	1.81e-51	177
MS.gene66845.t1	AT1G26260	64.583	144	45	2	212	350	170	312	2.93e-51	177
MS.gene66845.t1	AT1G26260	64.583	144	45	2	212	350	170	312	2.93e-51	177
MS.gene66845.t1	AT1G26260	64.583	144	45	2	212	350	170	312	2.93e-51	177
MS.gene66845.t1	AT1G26260	64.583	144	45	2	212	350	170	312	2.93e-51	177
MS.gene66845.t1	AT3G07340	64.964	137	42	3	218	350	213	347	2.49e-48	171
MS.gene66845.t1	AT3G07340	64.964	137	42	3	218	350	213	347	3.16e-48	171
MS.gene66845.t1	AT3G23690	54.598	174	65	5	245	408	176	345	2.40e-46	164
MS.gene66845.t1	AT4G34530	48.256	172	74	2	191	347	87	258	1.12e-43	156
MS.gene66845.t1	AT5G48560	77.419	93	20	1	257	348	297	389	5.96e-43	157
MS.gene66845.t1	AT5G50915	51.163	172	61	5	225	396	106	254	1.03e-42	151
MS.gene66845.t1	AT5G50915	51.163	172	61	5	225	396	106	254	1.03e-42	151
MS.gene66845.t1	AT5G48560	77.419	93	20	1	257	348	297	389	1.09e-42	157
MS.gene66845.t1	AT5G50915	51.163	172	61	5	225	396	106	254	1.62e-42	151
MS.gene66845.t1	AT5G50915	51.163	172	61	5	225	396	106	254	1.62e-42	151
MS.gene66845.t1	AT4G36540	51.316	152	68	3	195	345	79	225	1.96e-40	146
MS.gene66845.t1	AT4G36540	51.316	152	68	3	195	345	79	225	2.06e-40	146
MS.gene66845.t1	AT1G59640	53.623	138	57	2	212	349	94	224	3.99e-40	144
MS.gene66845.t1	AT2G18300	54.610	141	46	2	218	340	124	264	6.43e-40	145
MS.gene66845.t1	AT1G59640	53.285	137	57	2	212	348	94	223	6.45e-40	146
MS.gene66845.t1	AT2G18300	54.610	141	46	2	218	340	124	264	7.16e-40	145
MS.gene66845.t1	AT2G18300	54.610	141	46	2	218	340	124	264	1.21e-39	145
MS.gene66845.t1	AT3G57800	37.066	259	124	5	126	346	32	289	1.40e-39	146
MS.gene66845.t1	AT2G42300	62.500	96	36	0	252	347	176	271	1.52e-37	138
MS.gene66845.t1	AT2G42300	63.158	95	35	0	252	346	176	270	1.23e-36	137
MS.gene66845.t1	AT5G62610	76.316	76	18	0	257	332	149	224	4.75e-35	131
MS.gene66845.t1	AT5G62610	76.316	76	18	0	257	332	149	224	4.75e-35	131
MS.gene66845.t1	AT1G18400	70.732	82	24	0	259	340	143	224	6.13e-33	124
MS.gene66845.t1	AT1G18400	70.732	82	24	0	259	340	143	224	9.38e-33	124
MS.gene66845.t1	AT1G73830	69.136	81	25	0	260	340	146	226	8.23e-32	122
MS.gene66845.t1	AT1G73830	69.136	81	25	0	260	340	146	226	8.51e-32	122
MS.gene66845.t1	AT1G25330	50.806	124	50	1	257	369	100	223	2.19e-31	120
MS.gene66845.t1	AT3G57800	31.373	306	124	6	126	346	32	336	5.73e-31	123
MS.gene66845.t1	AT2G24260	69.697	66	19	1	261	326	35	99	2.82e-22	95.5
MS.gene66845.t1	AT5G58010	62.963	81	25	2	260	336	98	177	3.32e-22	96.7
MS.gene66845.t1	AT4G30980	71.212	66	18	1	261	326	130	194	6.21e-22	95.9
MS.gene66845.t1	AT4G30980	71.212	66	18	1	261	326	178	242	1.01e-21	96.3
MS.gene66845.t1	AT2G24260	69.697	66	19	1	261	326	138	202	1.36e-21	95.9
MS.gene66845.t1	AT2G42300	68.000	50	16	0	252	301	176	225	3.82e-17	80.5
MS.gene66845.t1	AT2G42300	68.000	50	16	0	252	301	176	225	3.82e-17	80.5
MS.gene66845.t1	AT2G42300	69.388	49	15	0	252	300	176	224	4.34e-17	80.5
MS.gene66845.t1	AT4G09180	42.056	107	49	3	241	334	153	259	1.33e-15	77.0
MS.gene66845.t1	AT1G35460	53.731	67	31	0	259	325	179	245	1.75e-15	76.6
MS.gene66845.t1	AT2G20180	41.053	95	55	1	232	326	178	271	1.94e-15	78.2
MS.gene66845.t1	AT2G20180	41.053	95	55	1	232	326	178	271	1.94e-15	78.2
MS.gene66845.t1	AT2G20180	41.053	95	55	1	232	326	178	271	1.94e-15	78.2
MS.gene66845.t1	AT2G20180	41.053	95	55	1	232	326	249	342	2.49e-15	78.2
MS.gene66845.t1	AT2G20180	41.053	95	55	1	232	326	249	342	2.49e-15	78.2
MS.gene66845.t1	AT2G20180	41.053	95	55	1	232	326	249	342	2.49e-15	78.2
MS.gene66845.t1	AT2G20180	41.053	95	55	1	232	326	249	342	2.49e-15	78.2
MS.gene66845.t1	AT2G42280	54.688	64	29	0	261	324	279	342	3.36e-15	77.0
MS.gene66845.t1	AT1G09530	53.125	64	29	1	263	326	339	401	4.95e-15	77.4
MS.gene66845.t1	AT1G09530	53.125	64	29	1	263	326	339	401	4.95e-15	77.4
MS.gene66845.t1	AT1G09530	53.125	64	29	1	263	326	339	401	4.95e-15	77.4
MS.gene66845.t1	AT1G09530	53.125	64	29	1	263	326	339	401	4.95e-15	77.4
MS.gene66845.t1	AT1G09530	53.125	64	29	1	263	326	339	401	4.95e-15	77.4
MS.gene66845.t1	AT1G09530	53.125	64	29	1	263	326	339	401	4.95e-15	77.4
MS.gene66845.t1	AT2G42280	56.061	66	27	1	261	324	279	344	5.87e-15	76.3
MS.gene66845.t1	AT1G51140	56.250	64	28	0	261	324	304	367	7.51e-15	76.3
MS.gene66845.t1	AT4G28790	35.156	128	64	3	211	333	229	342	1.73e-14	75.5
MS.gene66845.t1	AT2G20180	40.217	92	54	1	232	323	249	339	5.30e-14	73.2
MS.gene66845.t1	AT4G02590	64.179	67	23	1	260	326	82	147	7.06e-14	71.6
MS.gene66845.t1	AT4G02590	64.179	67	23	1	260	326	145	210	9.43e-14	72.0
MS.gene66845.t1	AT4G02590	64.179	67	23	1	260	326	145	210	9.43e-14	72.0
MS.gene66845.t1	AT4G28815	55.172	58	25	1	264	321	71	127	1.06e-13	70.9
MS.gene66845.t1	AT4G28800	35.338	133	68	6	264	389	252	373	1.19e-13	72.8
MS.gene66845.t1	AT1G03040	64.179	67	23	1	260	326	138	203	1.38e-13	71.6
MS.gene66845.t1	AT1G03040	65.152	66	22	1	261	326	144	208	1.62e-13	71.2
MS.gene66845.t1	AT1G03040	64.179	67	23	1	260	326	143	208	2.83e-13	70.9
MS.gene66845.t1	AT4G28815	55.172	58	25	1	264	321	147	203	3.60e-13	70.5
MS.gene66845.t1	AT4G28790	35.897	117	56	3	211	322	229	331	5.12e-13	70.1
MS.gene66845.t1	AT4G28800	52.542	59	27	1	264	322	264	321	3.34e-12	68.6
MS.gene66845.t1	AT4G00050	49.231	65	32	1	261	325	105	168	7.30e-12	66.2
MS.gene66845.t1	AT2G46970	41.250	80	41	2	259	333	216	294	1.19e-11	66.6
MS.gene66845.t1	AT2G46970	41.250	80	41	2	259	333	185	263	1.33e-11	66.2
MS.gene66845.t1	AT2G46970	41.250	80	41	2	259	333	194	272	1.40e-11	66.2
MS.gene66845.t1	AT4G00050	49.231	65	32	1	261	325	207	270	1.50e-11	66.2
MS.gene66845.t1	AT4G33880	49.231	65	32	1	261	325	266	329	1.67e-11	65.9
MS.gene66845.t1	AT4G00050	49.231	65	32	1	261	325	207	270	1.67e-11	65.9
MS.gene66845.t1	AT2G43140	48.485	66	34	0	259	324	229	294	2.04e-11	65.1
MS.gene66845.t1	AT2G43140	48.485	66	34	0	259	324	132	197	4.08e-11	62.8
MS.gene66845.t1	AT4G36930	49.180	61	30	1	267	327	197	256	9.55e-11	63.5
MS.gene66845.t1	AT1G27740	31.206	141	87	3	192	325	93	230	9.85e-11	62.4

Find 107 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAATACAGCACTTGACAATT+TGG	0.175917	1.2:+5502066	MS.gene66845:CDS
TTCCCTGATTGTTCAACTTT+TGG	0.193603	1.2:-5500613	None:intergenic
ATCTTATAACCCTCTTGTTT+TGG	0.236824	1.2:+5499778	MS.gene66845:CDS
TTGAGTCAAGCTCATACTTT+TGG	0.246530	1.2:+5499710	MS.gene66845:CDS
AAGATCTTCACTTACATCTT+TGG	0.264618	1.2:-5501485	None:intergenic
TTCTTCTCTTATTAGATAAC+TGG	0.273700	1.2:+5501065	MS.gene66845:intron
TCAGAAATGGTTGGTTCCTT+TGG	0.277793	1.2:+5499896	MS.gene66845:CDS
GCTTAGAAACAAACCTTGTT+TGG	0.285521	1.2:-5500139	None:intergenic
GGCATTGGTGGATTTGTTCC+TGG	0.308014	1.2:+5501817	MS.gene66845:CDS
TATTAGTTCTGTGTCAAATA+TGG	0.309763	1.2:+5499598	MS.gene66845:CDS
ATCCAAAGGGGTCAAATTAA+TGG	0.316200	1.2:+5500007	MS.gene66845:CDS
TTGCTTCAAGAACTTGTTCC+AGG	0.318899	1.2:+5500882	MS.gene66845:CDS
TGCAAGTTTCCAGGGTAATA+TGG	0.324788	1.2:+5501867	MS.gene66845:CDS
TGCAAGACTGTGACTGTTTG+TGG	0.339736	1.2:-5500713	None:intergenic
CAAATTGTCAAGTGCTGTAT+TGG	0.339868	1.2:-5502065	None:intergenic
CTTGTTCAATACTCAAATCT+TGG	0.340230	1.2:+5499830	MS.gene66845:CDS
GCAGCAGCAGCATCATTTCA+TGG	0.345728	1.2:+5499655	MS.gene66845:CDS
CACCATTAATTTGACCCCTT+TGG	0.347434	1.2:-5500009	None:intergenic
CGCTTAGGACTCGGCATTGG+TGG	0.369113	1.2:+5501805	MS.gene66845:CDS
TCCTTTGGCCAACATGGGTC+AGG	0.379373	1.2:+5499911	MS.gene66845:CDS
TACCCTGGAAACTTGCATTT+GGG	0.381334	1.2:-5501861	None:intergenic
TTACCCTGGAAACTTGCATT+TGG	0.385009	1.2:-5501862	None:intergenic
ATTCCCAAATGCAAGTTTCC+AGG	0.387230	1.2:+5501858	MS.gene66845:CDS
AAACCAAAAGTTGAACAATC+AGG	0.388796	1.2:+5500610	MS.gene66845:CDS
ATTTCATGGCTTCTTCAGCT+TGG	0.391101	1.2:+5499669	MS.gene66845:CDS
CTACATCAAAGTTCAGTTCC+GGG	0.392601	1.2:-5501450	None:intergenic
TTCTTGTCCATCTTCAGAAA+TGG	0.392622	1.2:+5499574	MS.gene66845:CDS
TCTACATCAAAGTTCAGTTC+CGG	0.395058	1.2:-5501451	None:intergenic
TTTCATGGCTTCTTCAGCTT+GGG	0.397346	1.2:+5499670	MS.gene66845:CDS
AAGTGGAAGTTTCTCAGAAA+TGG	0.401403	1.2:+5499883	MS.gene66845:CDS
AATACAGCACTTGACAATTT+GGG	0.404050	1.2:+5502067	MS.gene66845:CDS
TTATAATGCTGTGATGGAAT+TGG	0.423796	1.2:-5499965	None:intergenic
TATGTACATGCAGAATGCTT+TGG	0.428401	1.2:+5502003	MS.gene66845:intron
AAATTGTCAAGTGCTGTATT+GGG	0.430882	1.2:-5502064	None:intergenic
TTTGGCCAACATGGGTCAGG+TGG	0.434581	1.2:+5499914	MS.gene66845:CDS
TGCCGAGTCCTAAGCGTGAC+TGG	0.437410	1.2:-5501798	None:intergenic
CTCCAGTCACGCTTAGGACT+CGG	0.439169	1.2:+5501796	MS.gene66845:CDS
CAGTCACAGTCTTGCAGAAA+GGG	0.448205	1.2:+5500720	MS.gene66845:CDS
ATCGCTACCTTGTTGCATCC+TGG	0.450261	1.2:-5500900	None:intergenic
GTTGGTTCCTTTGGCCAACA+TGG	0.452089	1.2:+5499905	MS.gene66845:CDS
GAACCATCAGAAATCTTCCT+TGG	0.455036	1.2:-5500571	None:intergenic
GAACATGTAGCTAACACATC+AGG	0.456379	1.2:+5499938	MS.gene66845:CDS
ACAGGATCTGCACCTGGTAG+TGG	0.456742	1.2:+5500073	MS.gene66845:CDS
ATTCATGAGGATGGAGGTAC+AGG	0.461324	1.2:+5500055	MS.gene66845:CDS
CTAATATCCATTTCTGAAGA+TGG	0.462754	1.2:-5499581	None:intergenic
ATCCACAAGATTCCTTCCTA+TGG	0.466206	1.2:+5499860	MS.gene66845:CDS
TATTCAACACACTATAACTC+TGG	0.473092	1.2:-5502475	None:intergenic
AAAGTTCAGTTCCGGGTTCA+CGG	0.473106	1.2:-5501443	None:intergenic
TAGATTCTCCAGTCACGCTT+AGG	0.473536	1.2:+5501790	MS.gene66845:intron
ACAGTTCCCTCCTTTGCCTC+AGG	0.473617	1.2:+5501912	MS.gene66845:CDS
TGTGCAATCACTGCAACAAC+AGG	0.487175	1.2:+5501121	MS.gene66845:CDS
TATAATCACATCAAGGATGC+TGG	0.503047	1.2:+5499983	MS.gene66845:CDS
GAAACTTGCCACCGTGAACC+CGG	0.509079	1.2:+5501432	MS.gene66845:CDS
CAAGGATGCTGGAATCCAAA+GGG	0.509335	1.2:+5499994	MS.gene66845:CDS
GTTCAATACTCAAATCTTGG+AGG	0.510892	1.2:+5499833	MS.gene66845:CDS
AAGATTCCTTCCTATGGAAG+TGG	0.511892	1.2:+5499866	MS.gene66845:CDS
TGAGAAACTTCCACTTCCAT+AGG	0.519524	1.2:-5499876	None:intergenic
GGGAACTGTGTCGATGAACT+AGG	0.526485	1.2:-5501898	None:intergenic
AAAAGATTAGTGAACGAATG+AGG	0.527173	1.2:+5500859	MS.gene66845:CDS
ACAGTCACAGTCTTGCAGAA+AGG	0.528577	1.2:+5500719	MS.gene66845:CDS
TGGAAACTTGCATTTGGGAA+TGG	0.530239	1.2:-5501856	None:intergenic
TTTCTTATACTTCCACTACC+AGG	0.533484	1.2:-5500085	None:intergenic
GGAAGTTTCTCAGAAATGGT+TGG	0.538496	1.2:+5499887	MS.gene66845:CDS
TTGGTTCCTTTGGCCAACAT+GGG	0.538891	1.2:+5499906	MS.gene66845:CDS
TTCCCAAATGCAAGTTTCCA+GGG	0.541363	1.2:+5501859	MS.gene66845:CDS
GATTAGAATGCTGAGGGTGA+TGG	0.542730	1.2:+5500535	MS.gene66845:intron
AGTTTCCAGGGTAATATGGC+TGG	0.546057	1.2:+5501871	MS.gene66845:CDS
TTCTACTTTCAGTCCAAACA+AGG	0.550639	1.2:+5500126	MS.gene66845:CDS
TTGGCCAACATGGGTCAGGT+GGG	0.552278	1.2:+5499915	MS.gene66845:CDS
AACCAAAAGTTGAACAATCA+GGG	0.556875	1.2:+5500611	MS.gene66845:CDS
TCTCCAAGGAAGATTTCTGA+TGG	0.568713	1.2:+5500568	MS.gene66845:CDS
ACCTGACCCATGTTGGCCAA+AGG	0.569057	1.2:-5499912	None:intergenic
GGATGAGAAGAGCTTATACC+AGG	0.576202	1.2:-5501835	None:intergenic
TGGTGAACAATCTCAGATTG+AGG	0.578059	1.2:+5500027	MS.gene66845:CDS
AACTGAACTTTGATGTAGAA+CGG	0.586015	1.2:+5501455	MS.gene66845:CDS
TCACGCTTAGGACTCGGCAT+TGG	0.589627	1.2:+5501802	MS.gene66845:CDS
TCAAGGATGCTGGAATCCAA+AGG	0.589904	1.2:+5499993	MS.gene66845:CDS
ATGTGATTATAATGCTGTGA+TGG	0.590061	1.2:-5499971	None:intergenic
GAGGACTCTATTCATGAGGA+TGG	0.590188	1.2:+5500046	MS.gene66845:CDS
CCTTGATTCTCCAAAACAAG+AGG	0.592951	1.2:-5499788	None:intergenic
AAACTTCCACTTCCATAGGA+AGG	0.598638	1.2:-5499872	None:intergenic
GCTTGACTCAATGAAACAAG+TGG	0.600555	1.2:-5499698	None:intergenic
TTCCATAGGAAGGAATCTTG+TGG	0.606199	1.2:-5499862	None:intergenic
GGGTGGTGAGGAAAATGCAA+TGG	0.606665	1.2:+5499520	MS.gene66845:CDS
TCTTTAGAAGCATCTCCACT+CGG	0.607421	1.2:-5500655	None:intergenic
CTTGACAATTTGGGACCCAA+TGG	0.608459	1.2:+5502076	MS.gene66845:CDS
TGGCCAACATGGGTCAGGTG+GGG	0.608930	1.2:+5499916	MS.gene66845:CDS
CTTGATTCTCCAAAACAAGA+GGG	0.612056	1.2:-5499787	None:intergenic
ACAGCATTATAATCACATCA+AGG	0.612365	1.2:+5499976	MS.gene66845:CDS
ATCACTGCAACAACAGGTTG+AGG	0.619338	1.2:+5501127	MS.gene66845:CDS
GTTCCCCACCTGACCCATGT+TGG	0.625912	1.2:-5499919	None:intergenic
GAATTAAGAGATGGGTGGTG+AGG	0.626693	1.2:+5499508	None:intergenic
AACATGTAGCTAACACATCA+GGG	0.630115	1.2:+5499939	MS.gene66845:CDS
GAACTTGGATTAGAATGCTG+AGG	0.632192	1.2:+5500528	MS.gene66845:intron
GGAGGTACAGGATCTGCACC+TGG	0.632655	1.2:+5500067	MS.gene66845:CDS
AACTTGGATTAGAATGCTGA+GGG	0.637008	1.2:+5500529	MS.gene66845:intron
ATGGAGTGAAAGATTCTCCA+AGG	0.638409	1.2:+5500554	MS.gene66845:CDS
GATTGAGGACTCTATTCATG+AGG	0.640742	1.2:+5500042	MS.gene66845:CDS
GCATGCCAGCCATATTACCC+TGG	0.643474	1.2:-5501876	None:intergenic
AATGCAATGGAGTTTCAACA+TGG	0.644756	1.2:+5499533	MS.gene66845:CDS
ATTCATGTGAGAGCTAGAAG+AGG	0.658128	1.2:+5500685	MS.gene66845:CDS
GACTCTATTCATGAGGATGG+AGG	0.659616	1.2:+5500049	MS.gene66845:CDS
TTCTTGAATTAAGAGATGGG+TGG	0.661303	1.2:+5499503	None:intergenic
ACTTGTTCCAGGATGCAACA+AGG	0.687844	1.2:+5500893	MS.gene66845:CDS
AAGGATGCTGGAATCCAAAG+GGG	0.724973	1.2:+5499995	MS.gene66845:CDS
GCTAAAGATAACTCTCCGAG+TGG	0.729396	1.2:+5500640	MS.gene66845:CDS
GTTCAGTTCCGGGTTCACGG+TGG	0.752023	1.2:-5501440	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAATATATCAAATTTCAAAA+AGG	+	chr1.2:5502183-5502202	MS.gene66845:intron	15.0%
!!	AACCAAAAGTTATTAAAACT+TGG	-	chr1.2:5500817-5500836	None:intergenic	20.0%
!!	AATTTATTTCCATATTGAAC+AGG	+	chr1.2:5502442-5502461	MS.gene66845:intron	20.0%
!!	ACTAACAAAAAATACATTCA+TGG	+	chr1.2:5502271-5502290	MS.gene66845:intron	20.0%
!!	ATTTATTTCCATATTGAACA+GGG	+	chr1.2:5502443-5502462	MS.gene66845:intron	20.0%
!!	TATATCAATGAATCTCATAT+AGG	+	chr1.2:5500429-5500448	MS.gene66845:intron	20.0%
!!	TGAAATATCTTATATATGCT+TGG	+	chr1.2:5500366-5500385	MS.gene66845:intron	20.0%
!!!	AATCATAATTGTGTTTTAAC+TGG	-	chr1.2:5501747-5501766	None:intergenic	20.0%
!!!	TACCAAGTTTTAATAACTTT+TGG	+	chr1.2:5500812-5500831	MS.gene66845:intron	20.0%
!!!	TTAGTTGTTAATTTGTTAGA+GGG	+	chr1.2:5500222-5500241	MS.gene66845:intron	20.0%
!!!	TTTCAAAACTTTTATGGAAT+GGG	+	chr1.2:5502034-5502053	MS.gene66845:CDS	20.0%
!	AATGAATCTCATATAGGAAT+AGG	+	chr1.2:5500435-5500454	MS.gene66845:intron	25.0%
!	GTCGTAACTTATAACTTATT+TGG	+	chr1.2:5500962-5500981	MS.gene66845:intron	25.0%
!	GTTAAGATGATTGTGTTAAA+TGG	+	chr1.2:5500926-5500945	MS.gene66845:intron	25.0%
!	GTTTAATTGTATTGTCTATG+AGG	+	chr1.2:5500467-5500486	MS.gene66845:intron	25.0%
!	TAATCCAAGTTCATAATACA+AGG	-	chr1.2:5500520-5500539	None:intergenic	25.0%
!	TTACAGTATAAAGATGTTGA+TGG	-	chr1.2:5500759-5500778	None:intergenic	25.0%
!	TTCTTCTCTTATTAGATAAC+TGG	+	chr1.2:5501065-5501084	MS.gene66845:intron	25.0%
!!	ATTTTTATCCTTGAATGTGT+CGG	-	chr1.2:5502372-5502391	None:intergenic	25.0%
!!	CATGACTTTCAAAACTTTTA+TGG	+	chr1.2:5502028-5502047	MS.gene66845:CDS	25.0%
!!	CTTTCAAAACTTTTATGGAA+TGG	+	chr1.2:5502033-5502052	MS.gene66845:CDS	25.0%
!!	GCTTCAATTCCATTATATTT+TGG	+	chr1.2:5501359-5501378	MS.gene66845:intron	25.0%
!!	TTGATACTTTAAAGATTGCA+AGG	-	chr1.2:5501166-5501185	None:intergenic	25.0%
!!!	CATAAAAGTTTTGAAAGTCA+TGG	-	chr1.2:5502030-5502049	None:intergenic	25.0%
!!!	GTTAGTTGTTAATTTGTTAG+AGG	+	chr1.2:5500221-5500240	MS.gene66845:intron	25.0%
!!!	TATTAGTTCTGTGTCAAATA+TGG	+	chr1.2:5499598-5499617	MS.gene66845:CDS	25.0%
!!!	TTCCTTTTTTTTATTTCCCT+TGG	-	chr1.2:5501249-5501268	None:intergenic	25.0%
	AAAAGATTAGTGAACGAATG+AGG	+	chr1.2:5500859-5500878	MS.gene66845:CDS	30.0%
	AAACCAAAAGTTGAACAATC+AGG	+	chr1.2:5500610-5500629	MS.gene66845:CDS	30.0%
	AACCAAAAGTTGAACAATCA+GGG	+	chr1.2:5500611-5500630	MS.gene66845:CDS	30.0%
	AACTGAACTTTGATGTAGAA+CGG	+	chr1.2:5501455-5501474	MS.gene66845:CDS	30.0%
	AAGATCTTCACTTACATCTT+TGG	-	chr1.2:5501488-5501507	None:intergenic	30.0%
	AAGTGAAGATCTTAATCGTA+CGG	+	chr1.2:5501495-5501514	MS.gene66845:intron	30.0%
	AATACAGCACTTGACAATTT+GGG	+	chr1.2:5502067-5502086	MS.gene66845:CDS	30.0%
	AATGCCTTGTATTATGAACT+TGG	+	chr1.2:5500513-5500532	MS.gene66845:intron	30.0%
	ACAGCATTATAATCACATCA+AGG	+	chr1.2:5499976-5499995	MS.gene66845:CDS	30.0%
	ACTAGCAAAAGATACACTTA+AGG	-	chr1.2:5502210-5502229	None:intergenic	30.0%
	AGACATTGTTACTACAGTAT+CGG	+	chr1.2:5502161-5502180	MS.gene66845:intron	30.0%
	ATGTGATTATAATGCTGTGA+TGG	-	chr1.2:5499974-5499993	None:intergenic	30.0%
	CACCAAGGGAAATAAAAAAA+AGG	+	chr1.2:5501244-5501263	MS.gene66845:intron	30.0%
	CCAGGAAAACCAAAATATAA+TGG	-	chr1.2:5501371-5501390	None:intergenic	30.0%
	CTTGGTACACATTAAGAAAA+TGG	-	chr1.2:5500799-5500818	None:intergenic	30.0%
	CTTGTTCAATACTCAAATCT+TGG	+	chr1.2:5499830-5499849	MS.gene66845:CDS	30.0%
	GATATCAATGAATCTTATGC+AGG	+	chr1.2:5500177-5500196	MS.gene66845:intron	30.0%
	TCTTTATAAGATGAATAGCC+AGG	-	chr1.2:5501389-5501408	None:intergenic	30.0%
!	AAATTGTCAAGTGCTGTATT+GGG	-	chr1.2:5502067-5502086	None:intergenic	30.0%
!	AGTGGAAGTATAAGAAAAAG+AGG	+	chr1.2:5500091-5500110	MS.gene66845:CDS	30.0%
!	ATCTTTTGCTAGTCATTAAG+TGG	+	chr1.2:5502217-5502236	MS.gene66845:intron	30.0%
!	CTAATATCCATTTCTGAAGA+TGG	-	chr1.2:5499584-5499603	None:intergenic	30.0%
!	TTATAATGCTGTGATGGAAT+TGG	-	chr1.2:5499968-5499987	None:intergenic	30.0%
!!	ATCTTATAACCCTCTTGTTT+TGG	+	chr1.2:5499778-5499797	MS.gene66845:CDS	30.0%
!!!	ACTTTTGGTTTTGCTTTTGT+AGG	+	chr1.2:5500827-5500846	MS.gene66845:intron	30.0%
!!!	CCATTATATTTTGGTTTTCC+TGG	+	chr1.2:5501368-5501387	MS.gene66845:intron	30.0%
!!!	GTTGGTTTGTTTTGTTTATG+AGG	+	chr1.2:5500324-5500343	MS.gene66845:intron	30.0%
	AACATGTAGCTAACACATCA+GGG	+	chr1.2:5499939-5499958	MS.gene66845:CDS	35.0%
	AAGTGAACTATTCACTTTGG+AGG	+	chr1.2:5501532-5501551	MS.gene66845:intron	35.0%
	AAGTGAATAGTTCACTTCCA+CGG	-	chr1.2:5501529-5501548	None:intergenic	35.0%
	AAGTGGAAGTTTCTCAGAAA+TGG	+	chr1.2:5499883-5499902	MS.gene66845:CDS	35.0%
	AATGCAATGGAGTTTCAACA+TGG	+	chr1.2:5499533-5499552	MS.gene66845:CDS	35.0%
	ACTAATGAGAGACACATTTG+AGG	+	chr1.2:5502303-5502322	MS.gene66845:intron	35.0%
	AGATTCTGCCTATTTACCAT+TGG	-	chr1.2:5502095-5502114	None:intergenic	35.0%
	AGTCATTAAGTGGCTAATAG+AGG	+	chr1.2:5502227-5502246	MS.gene66845:intron	35.0%
	AGTGAACTATTCACTTTGGA+GGG	+	chr1.2:5501533-5501552	MS.gene66845:intron	35.0%
	ATCCAAAGGGGTCAAATTAA+TGG	+	chr1.2:5500007-5500026	MS.gene66845:CDS	35.0%
	ATGAGGCCATGATTGATATT+CGG	-	chr1.2:5501642-5501661	None:intergenic	35.0%
	ATGTGTCCGAATATCAATCA+TGG	+	chr1.2:5501633-5501652	MS.gene66845:intron	35.0%
	CAAATGTGTCTCTCATTAGT+CGG	-	chr1.2:5502303-5502322	None:intergenic	35.0%
	CAATACAGCACTTGACAATT+TGG	+	chr1.2:5502066-5502085	MS.gene66845:CDS	35.0%
	CCTGATTCATAGAACTTCAT+CGG	+	chr1.2:5500280-5500299	MS.gene66845:intron	35.0%
	CTCGATAGGATTTGTATTGT+TGG	+	chr1.2:5500306-5500325	MS.gene66845:intron	35.0%
	CTTGATTCTCCAAAACAAGA+GGG	-	chr1.2:5499790-5499809	None:intergenic	35.0%
	GATTCTGCCTATTTACCATT+GGG	-	chr1.2:5502094-5502113	None:intergenic	35.0%
	GCTTAGAAACAAACCTTGTT+TGG	-	chr1.2:5500142-5500161	None:intergenic	35.0%
	GTTCAATACTCAAATCTTGG+AGG	+	chr1.2:5499833-5499852	MS.gene66845:CDS	35.0%
	TAACCATATCGTTACATCGA+TGG	-	chr1.2:5501596-5501615	None:intergenic	35.0%
	TATAATCACATCAAGGATGC+TGG	+	chr1.2:5499983-5500002	MS.gene66845:CDS	35.0%
	TATGTACATGCAGAATGCTT+TGG	+	chr1.2:5502003-5502022	MS.gene66845:intron	35.0%
	TATTTCCATATTGAACAGGG+AGG	+	chr1.2:5502446-5502465	MS.gene66845:intron	35.0%
	TCTACATCAAAGTTCAGTTC+CGG	-	chr1.2:5501454-5501473	None:intergenic	35.0%
	TGGAAGTGAACTATTCACTT+TGG	+	chr1.2:5501529-5501548	MS.gene66845:intron	35.0%
	TTCTACTTTCAGTCCAAACA+AGG	+	chr1.2:5500126-5500145	MS.gene66845:CDS	35.0%
	TTCTTGTCCATCTTCAGAAA+TGG	+	chr1.2:5499574-5499593	MS.gene66845:CDS	35.0%
	TTTCTTATACTTCCACTACC+AGG	-	chr1.2:5500088-5500107	None:intergenic	35.0%
!	AACTTGGATTAGAATGCTGA+GGG	+	chr1.2:5500529-5500548	MS.gene66845:intron	35.0%
!	ATCTTTACTCTTTTCACCTG+AGG	-	chr1.2:5501931-5501950	None:intergenic	35.0%
!	CAAATTGTCAAGTGCTGTAT+TGG	-	chr1.2:5502068-5502087	None:intergenic	35.0%
!	TTCCCTGATTGTTCAACTTT+TGG	-	chr1.2:5500616-5500635	None:intergenic	35.0%
!	TTGAGTCAAGCTCATACTTT+TGG	+	chr1.2:5499710-5499729	MS.gene66845:CDS	35.0%
!!	TACTTTTGGAGGTTCTTCAA+TGG	+	chr1.2:5499724-5499743	MS.gene66845:CDS	35.0%
!!!	CTCTTGTTTTGGAGAATCAA+GGG	+	chr1.2:5499789-5499808	MS.gene66845:CDS	35.0%
	AAACTTCCACTTCCATAGGA+AGG	-	chr1.2:5499875-5499894	None:intergenic	40.0%
	AAGATTCCTTCCTATGGAAG+TGG	+	chr1.2:5499866-5499885	MS.gene66845:CDS	40.0%
	ATCCACAAGATTCCTTCCTA+TGG	+	chr1.2:5499860-5499879	MS.gene66845:CDS	40.0%
	ATGGAGTGAAAGATTCTCCA+AGG	+	chr1.2:5500554-5500573	MS.gene66845:CDS	40.0%
	ATTCATGTGAGAGCTAGAAG+AGG	+	chr1.2:5500685-5500704	MS.gene66845:CDS	40.0%
	ATTCCCAAATGCAAGTTTCC+AGG	+	chr1.2:5501858-5501877	MS.gene66845:CDS	40.0%
	ATTTCATGGCTTCTTCAGCT+TGG	+	chr1.2:5499669-5499688	MS.gene66845:CDS	40.0%
	CCGATGAAGTTCTATGAATC+AGG	-	chr1.2:5500283-5500302	None:intergenic	40.0%
	CCTTGATTCTCCAAAACAAG+AGG	-	chr1.2:5499791-5499810	None:intergenic	40.0%
	CTACATCAAAGTTCAGTTCC+GGG	-	chr1.2:5501453-5501472	None:intergenic	40.0%
	GAACATGTAGCTAACACATC+AGG	+	chr1.2:5499938-5499957	MS.gene66845:CDS	40.0%
	GAACCATCAGAAATCTTCCT+TGG	-	chr1.2:5500574-5500593	None:intergenic	40.0%
	GAATATCAATCATGGCCTCA+TGG	+	chr1.2:5501641-5501660	MS.gene66845:intron	40.0%
	GATTGAGGACTCTATTCATG+AGG	+	chr1.2:5500042-5500061	MS.gene66845:CDS	40.0%
	GCTTGACTCAATGAAACAAG+TGG	-	chr1.2:5499701-5499720	None:intergenic	40.0%
	GGAAGTTTCTCAGAAATGGT+TGG	+	chr1.2:5499887-5499906	MS.gene66845:CDS	40.0%
	TACCCTGGAAACTTGCATTT+GGG	-	chr1.2:5501864-5501883	None:intergenic	40.0%
	TAGCCATCGATGTAACGATA+TGG	+	chr1.2:5501590-5501609	MS.gene66845:intron	40.0%
	TCTCCAAGGAAGATTTCTGA+TGG	+	chr1.2:5500568-5500587	MS.gene66845:CDS	40.0%
	TGAGAAACTTCCACTTCCAT+AGG	-	chr1.2:5499879-5499898	None:intergenic	40.0%
	TGCAAGTTTCCAGGGTAATA+TGG	+	chr1.2:5501867-5501886	MS.gene66845:CDS	40.0%
	TGGAAACTTGCATTTGGGAA+TGG	-	chr1.2:5501859-5501878	None:intergenic	40.0%
	TGGTGAACAATCTCAGATTG+AGG	+	chr1.2:5500027-5500046	MS.gene66845:CDS	40.0%
	TTACCCTGGAAACTTGCATT+TGG	-	chr1.2:5501865-5501884	None:intergenic	40.0%
	TTCAACCTCCCTGTTCAATA+TGG	-	chr1.2:5502454-5502473	None:intergenic	40.0%
	TTCCATAGGAAGGAATCTTG+TGG	-	chr1.2:5499865-5499884	None:intergenic	40.0%
	TTCCCAAATGCAAGTTTCCA+GGG	+	chr1.2:5501859-5501878	MS.gene66845:CDS	40.0%
	TTTATCCTTGAATGTGTCGG+TGG	-	chr1.2:5502369-5502388	None:intergenic	40.0%
	TTTCATGGCTTCTTCAGCTT+GGG	+	chr1.2:5499670-5499689	MS.gene66845:CDS	40.0%
!	AGTCAAGCTCATACTTTTGG+AGG	+	chr1.2:5499713-5499732	MS.gene66845:CDS	40.0%
!	CACCATTAATTTGACCCCTT+TGG	-	chr1.2:5500012-5500031	None:intergenic	40.0%
!	GAACTTGGATTAGAATGCTG+AGG	+	chr1.2:5500528-5500547	MS.gene66845:intron	40.0%
!	TCAGAAATGGTTGGTTCCTT+TGG	+	chr1.2:5499896-5499915	MS.gene66845:CDS	40.0%
!	TTGCTTCAAGAACTTGTTCC+AGG	+	chr1.2:5500882-5500901	MS.gene66845:CDS	40.0%
!	TTTGGGACCCAATGGTAAAT+AGG	+	chr1.2:5502084-5502103	MS.gene66845:intron	40.0%
!!	AGTTGATGAGAAGATCCATG+AGG	-	chr1.2:5501659-5501678	None:intergenic	40.0%
!!	TCTTTAGAAGCATCTCCACT+CGG	-	chr1.2:5500658-5500677	None:intergenic	40.0%
!!!	CCTCTTGTTTTGGAGAATCA+AGG	+	chr1.2:5499788-5499807	MS.gene66845:CDS	40.0%
	AAAGTTCAGTTCCGGGTTCA+CGG	-	chr1.2:5501446-5501465	None:intergenic	45.0%
	AAGGATGCTGGAATCCAAAG+GGG	+	chr1.2:5499995-5500014	MS.gene66845:CDS	45.0%
	ACAGTCACAGTCTTGCAGAA+AGG	+	chr1.2:5500719-5500738	MS.gene66845:CDS	45.0%
	ACTTGTTCCAGGATGCAACA+AGG	+	chr1.2:5500893-5500912	MS.gene66845:CDS	45.0%
	AGTTTCCAGGGTAATATGGC+TGG	+	chr1.2:5501871-5501890	MS.gene66845:CDS	45.0%
	ATCACTGCAACAACAGGTTG+AGG	+	chr1.2:5501127-5501146	MS.gene66845:CDS	45.0%
	CAAGGATGCTGGAATCCAAA+GGG	+	chr1.2:5499994-5500013	MS.gene66845:CDS	45.0%
	CAGTCACAGTCTTGCAGAAA+GGG	+	chr1.2:5500720-5500739	MS.gene66845:CDS	45.0%
	GACTCTATTCATGAGGATGG+AGG	+	chr1.2:5500049-5500068	MS.gene66845:CDS	45.0%
	GAGGACTCTATTCATGAGGA+TGG	+	chr1.2:5500046-5500065	MS.gene66845:CDS	45.0%
	GATGCAACAAGGTAGCGATT+TGG	+	chr1.2:5500904-5500923	MS.gene66845:intron	45.0%
	GATTAGAATGCTGAGGGTGA+TGG	+	chr1.2:5500535-5500554	MS.gene66845:intron	45.0%
	GCTAAAGATAACTCTCCGAG+TGG	+	chr1.2:5500640-5500659	MS.gene66845:CDS	45.0%
	GGATGAGAAGAGCTTATACC+AGG	-	chr1.2:5501838-5501857	None:intergenic	45.0%
	GTGCACATGCTTAACACCAA+GGG	+	chr1.2:5501230-5501249	MS.gene66845:intron	45.0%
	TAGATTCTCCAGTCACGCTT+AGG	+	chr1.2:5501790-5501809	MS.gene66845:intron	45.0%
	TCAAGGATGCTGGAATCCAA+AGG	+	chr1.2:5499993-5500012	MS.gene66845:CDS	45.0%
	TGCAAGACTGTGACTGTTTG+TGG	-	chr1.2:5500716-5500735	None:intergenic	45.0%
	TGTGCAATCACTGCAACAAC+AGG	+	chr1.2:5501121-5501140	MS.gene66845:CDS	45.0%
	TGTGCACATGCTTAACACCA+AGG	+	chr1.2:5501229-5501248	MS.gene66845:intron	45.0%
	TTGGTTCCTTTGGCCAACAT+GGG	+	chr1.2:5499906-5499925	MS.gene66845:CDS	45.0%
!	AACTTCATCGGTGTCTCGAT+AGG	+	chr1.2:5500292-5500311	MS.gene66845:intron	45.0%
!	ACTCTTTTCACCTGAGGCAA+AGG	-	chr1.2:5501925-5501944	None:intergenic	45.0%
!	ATTCATGAGGATGGAGGTAC+AGG	+	chr1.2:5500055-5500074	MS.gene66845:CDS	45.0%
!	CTTGACAATTTGGGACCCAA+TGG	+	chr1.2:5502076-5502095	MS.gene66845:CDS	45.0%
!	TTTTCACCTGAGGCAAAGGA+GGG	-	chr1.2:5501921-5501940	None:intergenic	45.0%
!!!	ACTTTTATAAAATAATATAA+AGG	+	chr1.2:5501271-5501290	MS.gene66845:intron	5.0%
	ATCGCTACCTTGTTGCATCC+TGG	-	chr1.2:5500903-5500922	None:intergenic	50.0%
	ATCGTACGGATTTGACTCCG+TGG	+	chr1.2:5501509-5501528	MS.gene66845:intron	50.0%
	CGATACCACCGACACATTCA+AGG	+	chr1.2:5502361-5502380	MS.gene66845:intron	50.0%
	GCAGCAGCAGCATCATTTCA+TGG	+	chr1.2:5499655-5499674	MS.gene66845:CDS	50.0%
	GGGTGGTGAGGAAAATGCAA+TGG	+	chr1.2:5499520-5499539	MS.gene66845:CDS	50.0%
!	CTTTTCACCTGAGGCAAAGG+AGG	-	chr1.2:5501922-5501941	None:intergenic	50.0%
!	GGGAACTGTGTCGATGAACT+AGG	-	chr1.2:5501901-5501920	None:intergenic	50.0%
!	GTTGGTTCCTTTGGCCAACA+TGG	+	chr1.2:5499905-5499924	MS.gene66845:CDS	50.0%
!!	GGCATTGGTGGATTTGTTCC+TGG	+	chr1.2:5501817-5501836	MS.gene66845:CDS	50.0%
	ACAGGATCTGCACCTGGTAG+TGG	+	chr1.2:5500073-5500092	MS.gene66845:CDS	55.0%
	ACAGTTCCCTCCTTTGCCTC+AGG	+	chr1.2:5501912-5501931	MS.gene66845:CDS	55.0%
	ACCTGACCCATGTTGGCCAA+AGG	-	chr1.2:5499915-5499934	None:intergenic	55.0%
	CTCCAGTCACGCTTAGGACT+CGG	+	chr1.2:5501796-5501815	MS.gene66845:CDS	55.0%
	GAAACTTGCCACCGTGAACC+CGG	+	chr1.2:5501432-5501451	MS.gene66845:CDS	55.0%
	GCATGCCAGCCATATTACCC+TGG	-	chr1.2:5501879-5501898	None:intergenic	55.0%
	TCACGCTTAGGACTCGGCAT+TGG	+	chr1.2:5501802-5501821	MS.gene66845:CDS	55.0%
	TCCTTTGGCCAACATGGGTC+AGG	+	chr1.2:5499911-5499930	MS.gene66845:CDS	55.0%
	TTGGCCAACATGGGTCAGGT+GGG	+	chr1.2:5499915-5499934	MS.gene66845:CDS	55.0%
	TTTGGCCAACATGGGTCAGG+TGG	+	chr1.2:5499914-5499933	MS.gene66845:CDS	55.0%
	GTTCAGTTCCGGGTTCACGG+TGG	-	chr1.2:5501443-5501462	None:intergenic	60.0%
	GTTCCCCACCTGACCCATGT+TGG	-	chr1.2:5499922-5499941	None:intergenic	60.0%
	TGCCGAGTCCTAAGCGTGAC+TGG	-	chr1.2:5501801-5501820	None:intergenic	60.0%
	TGGCCAACATGGGTCAGGTG+GGG	+	chr1.2:5499916-5499935	MS.gene66845:CDS	60.0%
!	CGCTTAGGACTCGGCATTGG+TGG	+	chr1.2:5501805-5501824	MS.gene66845:CDS	60.0%
!	GGAGGTACAGGATCTGCACC+TGG	+	chr1.2:5500067-5500086	MS.gene66845:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	5499518	5502486	5499518	ID=MS.gene66845
chr1.2	mRNA	5499518	5502486	5499518	ID=MS.gene66845.t1;Parent=MS.gene66845
chr1.2	exon	5499518	5500147	5499518	ID=MS.gene66845.t1.exon1;Parent=MS.gene66845.t1
chr1.2	CDS	5499518	5500147	5499518	ID=cds.MS.gene66845.t1;Parent=MS.gene66845.t1
chr1.2	exon	5500541	5500741	5500541	ID=MS.gene66845.t1.exon2;Parent=MS.gene66845.t1
chr1.2	CDS	5500541	5500741	5500541	ID=cds.MS.gene66845.t1;Parent=MS.gene66845.t1
chr1.2	exon	5500849	5500914	5500849	ID=MS.gene66845.t1.exon3;Parent=MS.gene66845.t1
chr1.2	CDS	5500849	5500914	5500849	ID=cds.MS.gene66845.t1;Parent=MS.gene66845.t1
chr1.2	exon	5501080	5501148	5501080	ID=MS.gene66845.t1.exon4;Parent=MS.gene66845.t1
chr1.2	CDS	5501080	5501148	5501080	ID=cds.MS.gene66845.t1;Parent=MS.gene66845.t1
chr1.2	exon	5501421	5501492	5501421	ID=MS.gene66845.t1.exon5;Parent=MS.gene66845.t1
chr1.2	CDS	5501421	5501492	5501421	ID=cds.MS.gene66845.t1;Parent=MS.gene66845.t1
chr1.2	exon	5501793	5501933	5501793	ID=MS.gene66845.t1.exon6;Parent=MS.gene66845.t1
chr1.2	CDS	5501793	5501933	5501793	ID=cds.MS.gene66845.t1;Parent=MS.gene66845.t1
chr1.2	exon	5502016	5502097	5502016	ID=MS.gene66845.t1.exon7;Parent=MS.gene66845.t1
chr1.2	CDS	5502016	5502097	5502016	ID=cds.MS.gene66845.t1;Parent=MS.gene66845.t1
chr1.2	exon	5502464	5502486	5502464	ID=MS.gene66845.t1.exon8;Parent=MS.gene66845.t1
chr1.2	CDS	5502464	5502486	5502464	ID=cds.MS.gene66845.t1;Parent=MS.gene66845.t1

Gene Sequence

>MS.gene66845

ATGGGTGGTGAGGAAAATGCAATGGAGTTTCAACATGGAAATGAGAATATTATGAGTTCTTGTCCATCTTCAGAAATGGATATTAGTTCTGTGTCAAATATGGCTAAGACTTCTTCAGATGTAGTTAATCATCATCAGCAGCAGCAGCATCATTTCATGGCTTCTTCAGCTTGGGATAATCCACTTGTTTCATTGAGTCAAGCTCATACTTTTGGAGGTTCTTCAATGGTTTCTCATAATGAGTTTGCTAATGCAAATTCATCTTATAACCCTCTTGTTTTGGAGAATCAAGGGATGAGTAGCACTTCTCATCTTGTTCAATACTCAAATCTTGGAGGCATGATCCACAAGATTCCTTCCTATGGAAGTGGAAGTTTCTCAGAAATGGTTGGTTCCTTTGGCCAACATGGGTCAGGTGGGGAACATGTAGCTAACACATCAGGGTATCCAATTCCATCACAGCATTATAATCACATCAAGGATGCTGGAATCCAAAGGGGTCAAATTAATGGTGAACAATCTCAGATTGAGGACTCTATTCATGAGGATGGAGGTACAGGATCTGCACCTGGTAGTGGAAGTATAAGAAAAAGAGGACTTGATCAAAATTCTACTTTCAGTCCAAACAAGGTTTGTTTCTAAGCTAAATTTGATATTCAGATATCAATGAATCTTATGCAGGAATTTTTCTTAATAGATGAATGTTAGTTGTTAATTTGTTAGAGGGAGAGAGAAATGTTAGTAGTAGAGATTGAACTTAGACCTGATTCATAGAACTTCATCGGTGTCTCGATAGGATTTGTATTGTTGGTTTGTTTTGTTTATGAGGTTTATGAGATACTAATGAATGAAATATCTTATATATGCTTGGATTAGATCAAACTTTGTTGCTAAGCTAAGTTTGATATTCTTATATCAATGAATCTCATATAGGAATAGGACTTGTAATGTTTAATTGTATTGTCTATGAGGTTTTATGAGATACTAATGCGAGAAATGCCTTGTATTATGAACTTGGATTAGAATGCTGAGGGTGATGGAGTGAAAGATTCTCCAAGGAAGATTTCTGATGGTTCAAAAGAACATGATAAGAAACCAAAAGTTGAACAATCAGGGAAGCAAGCTAAAGATAACTCTCCGAGTGGAGATGCTTCTAAAGATAATTTCATTCATGTGAGAGCTAGAAGAGGTCAAGCCACAAACAGTCACAGTCTTGCAGAAAGGGTACAAACTACAAACCATCAACATCTTTATACTGTAATGTTTGTTATGATAAATCCATTTTCTTAATGTGTACCAAGTTTTAATAACTTTTGGTTTTGCTTTTGTAGGTTAGAAGAGAAAAGATTAGTGAACGAATGAGGTTGCTTCAAGAACTTGTTCCAGGATGCAACAAGGTAGCGATTTGGTTAAGATGATTGTGTTAAATGGTTTCTTATGATTCGTCGTAACTTATAACTTATTTGGTGTCACATTGATTGATATATGTCTCAGCAGATTAATTTCATTTTCTCTTTGTTAATTTTTCTGATGATTATGTCTTTGATTTCTTCTCTTATTAGATAACTGGCAAAGCAGTAATGCTAGATGAGATAATAAACTATGTGCAATCACTGCAACAACAGGTTGAGGTAAGACAGCAGTGCCTTGCAATCTTTAAAGTATCAATTTATTAGTATTATTCAAAGTTCAAACTTGATCTGTTTTGCACTGTGCACATGCTTAACACCAAGGGAAATAAAAAAAAGGAAATACTTTTATAAAATAATATAAAGGATTTAATCATTTCACTGAATAATGAATATTAAGAGATTGTTTTATGATAATTACATGACTTGAAAGCTTCAATTCCATTATATTTTGGTTTTCCTGGCTATTCATCTTATAAAGATATATTTTGCAGTTTTTGTCAATGAAACTTGCCACCGTGAACCCGGAACTGAACTTTGATGTAGAACGGCTCTTATCCAAAGATGTAAGTGAAGATCTTAATCGTACGGATTTGACTCCGTGGAAGTGAACTATTCACTTTGGAGGGTAAAGTAAATAATCTCAATTGTTGATGAGAAATTTAGCCATCGATGTAACGATATGGTTATGCATAGCAAATCTTAAATGTGTCCGAATATCAATCATGGCCTCATGGATCTTCTCATCAACTGTTGACATTGCTTTATCTTCTAAGTAAATTGTACACTTTAGAAGAGTTACTCTTGCTATCTTAGTCCAGTTAAAACACAATTATGATTATGACGTGTATTTCTCTATCAATTAGATTCTCCAGTCACGCTTAGGACTCGGCATTGGTGGATTTGTTCCTGGTATAAGCTCTTCTCATCCATTCCCAAATGCAAGTTTCCAGGGTAATATGGCTGGCATGCCTAGTTCATCGACACAGTTCCCTCCTTTGCCTCAGGTGAAAAGAGTAAAGATCTTTTCAACATTGTAGTATTAACTCACTTGTTTGTGAAGCACTGACATACATTATGTACATGCAGAATGCTTTGGACCATGACTTTCAAAACTTTTATGGAATGGGATATGATCCCAATACAGCACTTGACAATTTGGGACCCAATGGTAAATAGGCAGAATCTTTTTTTTTTTTTTTGAAAAACAGTCGAGAAATTTTCGAGATGTGCGAGACATTGTTACTACAGTATCGGAATATATCAAATTTCAAAAAGGTCCTTAAGTGTATCTTTTGCTAGTCATTAAGTGGCTAATAGAGGACACGTTCAAAGATCAAACTAACTAACAAAAAATACATTCATGGATCAAACCGACTAATGAGAGACACATTTGAGGTTGTTTTTATAATTTTTGTTAATTCAAGACTATAGCGATACCACCGACACATTCAAGGATAAAAATGACGATTTACTCTTCTTTCATTGTTTGCACGATATCAATTTTGTCTAATTAATTTATTTCCATATTGAACAGGGAGGTTGAAACCAGAGTTATAG

Protein sequence

>MS.gene66845.t1

MGGEENAMEFQHGNENIMSSCPSSEMDISSVSNMAKTSSDVVNHHQQQQHHFMASSAWDNPLVSLSQAHTFGGSSMVSHNEFANANSSYNPLVLENQGMSSTSHLVQYSNLGGMIHKIPSYGSGSFSEMVGSFGQHGSGGEHVANTSGYPIPSQHYNHIKDAGIQRGQINGEQSQIEDSIHEDGGTGSAPGSGSIRKRGLDQNSTFSPNKNAEGDGVKDSPRKISDGSKEHDKKPKVEQSGKQAKDNSPSGDASKDNFIHVRARRGQATNSHSLAERVRREKISERMRLLQELVPGCNKITGKAVMLDEIINYVQSLQQQVEFLSMKLATVNPELNFDVERLLSKDILQSRLGLGIGGFVPGISSSHPFPNASFQGNMAGMPSSSTQFPPLPQNALDHDFQNFYGMGYDPNTALDNLGPNGRLKPEL