AlfalfaGEDB | MS.gene67550

M. sativa cultivar XinJiangDaYe / MS.gene67550

KEGG: -
GO: GO:0003674 GO:0003824
PFAM: PF00501.31 PF13193.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67550.t1	RHN54425.1	97.6	532	11	1	1	532	26	555	2.50E-304	1053.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67550.t1	F4HUK6	45.4	533	273	8	9	532	9	532	5.8e-132	472.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67550.t1	G7K007	97.6	532	11	1	1	532	1	530	1.8e-304	1053.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050173	MS.gene67550	0.815992	6.96E-52	-1.69E-46
MS.gene051044	MS.gene67550	0.816073	6.67E-52	-1.69E-46
MS.gene051138	MS.gene67550	0.831825	1.34E-55	-1.69E-46
MS.gene051863	MS.gene67550	0.808307	3.30E-50	-1.69E-46
MS.gene053488	MS.gene67550	0.833444	5.31E-56	-1.69E-46
MS.gene053561	MS.gene67550	0.821466	3.98E-53	-1.69E-46
MS.gene054056	MS.gene67550	0.835012	2.15E-56	-1.69E-46
MS.gene055124	MS.gene67550	0.803189	3.93E-49	-1.69E-46
MS.gene055942	MS.gene67550	0.828908	6.92E-55	-1.69E-46
MS.gene058099	MS.gene67550	0.808058	3.73E-50	-1.69E-46
MS.gene059428	MS.gene67550	0.827941	1.18E-54	-1.69E-46
MS.gene059690	MS.gene67550	0.838399	2.94E-57	-1.69E-46
MS.gene059691	MS.gene67550	0.825489	4.56E-54	-1.69E-46
MS.gene059694	MS.gene67550	0.839729	1.33E-57	-1.69E-46
MS.gene059697	MS.gene67550	0.841492	4.60E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67550.t1	MTR_5g023730	97.556	532	11	1	1	532	1	530	0.0	1065
MS.gene67550.t1	MTR_5g008800	48.598	535	260	9	3	532	2	526	1.04e-176	509
MS.gene67550.t1	MTR_5g008820	47.081	531	266	8	6	532	5	524	9.26e-172	497
MS.gene67550.t1	MTR_8g074040	47.672	537	264	9	3	532	44	570	5.66e-170	494
MS.gene67550.t1	MTR_4g121600	42.153	548	292	10	3	532	5	545	3.48e-145	430
MS.gene67550.t1	MTR_8g074120	47.436	468	235	6	70	532	20	481	6.79e-143	422
MS.gene67550.t1	MTR_2g044180	39.755	571	307	11	1	541	3	566	9.50e-142	421
MS.gene67550.t1	MTR_5g076240	41.144	542	297	11	4	532	2	534	1.44e-140	418
MS.gene67550.t1	MTR_8g074040	46.405	459	231	7	81	532	2	452	4.51e-138	409
MS.gene67550.t1	MTR_3g064420	40.768	547	297	11	4	532	3	540	1.17e-135	406
MS.gene67550.t1	MTR_5g076220	42.754	552	293	12	4	541	2	544	1.76e-132	397
MS.gene67550.t1	MTR_5g076200	42.938	531	280	12	16	532	14	535	5.69e-128	385
MS.gene67550.t1	MTR_0161s0050	42.963	540	283	11	9	532	7	537	1.71e-126	382
MS.gene67550.t1	MTR_2g044180	38.462	481	262	9	89	541	1	475	2.02e-111	340
MS.gene67550.t1	MTR_0803s0020	26.993	552	337	16	16	540	235	747	1.07e-43	167
MS.gene67550.t1	MTR_5g007640	25.556	540	333	20	12	519	67	569	5.86e-32	130
MS.gene67550.t1	MTR_3g460770	25.862	522	327	19	8	506	16	500	7.60e-30	123
MS.gene67550.t1	MTR_8g039720	25.047	527	314	19	42	532	64	545	9.38e-30	123
MS.gene67550.t1	MTR_4g077900	26.008	496	317	16	55	532	1	464	1.27e-29	122
MS.gene67550.t1	MTR_1g026310	23.587	513	348	12	42	540	67	549	2.75e-29	122
MS.gene67550.t1	MTR_2g095510	25.560	536	354	17	16	540	27	528	3.18e-28	119
MS.gene67550.t1	MTR_1586s0010	24.873	394	273	10	15	396	39	421	1.16e-27	115
MS.gene67550.t1	MTR_4g057730	24.627	536	362	17	16	540	27	531	6.67e-27	114
MS.gene67550.t1	MTR_1g063110	24.074	540	365	16	16	540	28	537	1.31e-25	110
MS.gene67550.t1	MTR_4g005750	24.723	542	349	18	1	522	6	508	4.97e-25	109
MS.gene67550.t1	MTR_2g105570	25.202	496	321	19	43	522	54	515	1.35e-24	107
MS.gene67550.t1	MTR_5g027500	25.494	506	319	18	54	532	80	554	1.44e-24	107
MS.gene67550.t1	MTR_1g063120	24.248	532	346	19	16	522	28	527	1.36e-22	102
MS.gene67550.t1	MTR_1569s0010	28.286	350	227	10	193	533	188	522	1.44e-22	101
MS.gene67550.t1	MTR_5g027480	26.233	507	302	24	54	532	79	541	1.45e-22	101
MS.gene67550.t1	MTR_4g128337	23.412	551	358	17	12	540	29	537	2.14e-22	101
MS.gene67550.t1	MTR_4g057730	23.769	467	322	15	16	471	27	470	8.53e-22	99.0
MS.gene67550.t1	MTR_1g090737	23.930	514	315	18	38	524	79	543	5.54e-21	97.1
MS.gene67550.t1	MTR_3g035130	23.035	547	331	18	21	532	10	501	2.96e-20	94.4
MS.gene67550.t1	MTR_3g088880	23.499	383	231	12	184	540	199	545	7.09e-19	90.5
MS.gene67550.t1	MTR_2g030240	23.497	549	320	19	42	532	27	533	1.51e-18	89.4
MS.gene67550.t1	MTR_4g057730	23.333	450	311	15	16	454	27	453	5.44e-18	87.0
MS.gene67550.t1	MTR_3g088870	23.733	375	243	12	184	540	198	547	1.06e-17	86.7
MS.gene67550.t1	MTR_4g068670	21.926	488	304	17	44	472	79	548	4.87e-17	84.7
MS.gene67550.t1	MTR_1g073180	23.857	503	329	15	42	532	60	520	1.02e-15	80.5
MS.gene67550.t1	MTR_1054s0020	23.673	490	312	17	9	480	261	706	3.27e-15	79.3
MS.gene67550.t1	MTR_5g009360	23.092	511	293	20	35	472	68	551	4.06e-15	79.0
MS.gene67550.t1	MTR_8g099420	21.869	503	311	16	34	472	68	552	5.40e-15	78.6
MS.gene67550.t1	MTR_5g009360	24.740	384	208	15	151	472	61	425	7.79e-15	77.8
MS.gene67550.t1	MTR_5g009350	23.183	509	295	19	35	472	68	551	1.72e-14	76.6
MS.gene67550.t1	MTR_5g009350	23.183	509	295	19	35	472	68	551	2.05e-14	76.6
MS.gene67550.t1	MTR_1g026430	24.601	313	203	11	42	337	67	363	5.57e-14	74.3
MS.gene67550.t1	MTR_5g009350	23.077	507	294	19	35	470	68	549	6.49e-14	74.7
MS.gene67550.t1	MTR_1g073180	23.374	492	324	14	42	522	82	531	6.53e-14	74.7
MS.gene67550.t1	MTR_1g026470	24.920	313	202	11	42	337	67	363	7.43e-14	73.9
MS.gene67550.t1	MTR_5g009350	23.108	502	290	19	35	465	68	544	7.97e-14	74.7
MS.gene67550.t1	MTR_1g086600	33.793	145	86	4	329	472	438	573	1.13e-13	74.3
MS.gene67550.t1	MTR_7g109830	27.551	196	130	4	280	472	363	549	2.11e-13	73.6
MS.gene67550.t1	MTR_2g030240	24.378	402	237	15	181	532	55	439	1.21e-12	70.5
MS.gene67550.t1	MTR_7g084850	25.762	361	220	13	187	520	358	697	8.07e-12	68.6
MS.gene67550.t1	MTR_1g034360	28.481	158	91	6	322	472	408	550	1.98e-11	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67550.t1	AT1G20560	46.904	533	265	8	9	532	9	532	2.20e-172	499
MS.gene67550.t1	AT2G17650	46.741	537	258	10	15	532	60	587	3.83e-158	464
MS.gene67550.t1	AT5G16340	41.697	542	292	10	3	532	2	531	3.02e-141	419
MS.gene67550.t1	AT1G76290	43.017	537	285	11	3	532	2	524	9.38e-140	415
MS.gene67550.t1	AT1G66120	41.924	551	298	13	4	541	3	544	3.48e-139	414
MS.gene67550.t1	AT1G20560	45.435	460	234	7	82	532	3	454	7.99e-139	410
MS.gene67550.t1	AT3G16910	41.231	536	287	10	16	532	22	548	1.27e-137	410
MS.gene67550.t1	AT5G16370	41.144	542	295	10	3	532	2	531	1.93e-137	409
MS.gene67550.t1	AT1G75960	42.086	537	286	10	9	532	7	531	3.86e-136	406
MS.gene67550.t1	AT1G65880	39.216	561	307	11	4	541	3	552	9.79e-132	396
MS.gene67550.t1	AT1G21540	40.789	532	295	11	15	533	13	537	7.32e-131	392
MS.gene67550.t1	AT1G21530	39.736	531	301	10	15	533	13	536	7.72e-130	390
MS.gene67550.t1	AT1G65890	38.241	557	318	9	4	541	2	551	1.01e-129	390
MS.gene67550.t1	AT1G21530	39.850	532	297	12	15	533	13	534	1.14e-127	384
MS.gene67550.t1	AT1G77240	39.130	529	301	12	15	532	13	531	7.09e-125	377
MS.gene67550.t1	AT1G68270	35.420	559	303	12	4	542	2	522	8.24e-107	330
MS.gene67550.t1	AT1G65060	25.926	513	337	16	12	511	46	528	9.17e-28	117
MS.gene67550.t1	AT3G21240	25.341	513	335	16	43	540	64	543	1.11e-27	117
MS.gene67550.t1	AT5G63380	28.415	366	233	13	184	540	202	547	1.20e-26	114
MS.gene67550.t1	AT1G20510	24.638	552	348	18	9	532	19	530	5.66e-26	112
MS.gene67550.t1	AT4G05160	25.577	520	341	17	19	522	30	519	1.53e-25	110
MS.gene67550.t1	AT1G20500	26.962	497	304	19	62	540	84	539	2.02e-25	110
MS.gene67550.t1	AT3G21240	25.393	445	292	14	43	472	64	483	6.44e-25	108
MS.gene67550.t1	AT3G21240	25.393	445	292	14	43	472	64	483	6.44e-25	108
MS.gene67550.t1	AT1G20480	24.955	561	359	21	9	542	36	561	8.38e-25	108
MS.gene67550.t1	AT1G51680	25.358	489	325	16	40	513	64	527	2.57e-23	103
MS.gene67550.t1	AT1G51680	25.581	516	340	17	40	540	64	550	2.62e-23	104
MS.gene67550.t1	AT4G19010	24.000	500	325	19	62	541	93	557	5.49e-23	103
MS.gene67550.t1	AT1G20510	24.694	490	306	16	9	471	19	472	9.50e-23	101
MS.gene67550.t1	AT5G38120	25.192	520	331	17	25	524	46	527	1.31e-22	102
MS.gene67550.t1	AT1G51680	25.670	448	297	14	40	472	64	490	6.75e-22	99.4
MS.gene67550.t1	AT1G62940	23.855	524	335	15	34	540	53	529	7.29e-22	99.8
MS.gene67550.t1	AT5G38120	25.247	507	321	17	25	511	46	514	1.25e-21	98.6
MS.gene67550.t1	AT3G21230	24.719	534	349	16	12	522	40	543	5.80e-21	97.1
MS.gene67550.t1	AT5G38120	25.696	467	293	16	25	471	46	478	6.93e-21	96.3
MS.gene67550.t1	AT5G38120	25.696	467	293	16	25	471	46	478	6.93e-21	96.3
MS.gene67550.t1	AT3G21230	24.665	523	341	16	12	511	40	532	1.02e-20	96.3
MS.gene67550.t1	AT1G30520	23.665	562	359	17	22	540	13	547	5.20e-19	90.9
MS.gene67550.t1	AT1G30520	23.665	562	359	17	22	540	13	547	5.20e-19	90.9
MS.gene67550.t1	AT3G48990	24.457	552	328	17	21	540	9	503	1.13e-18	89.7
MS.gene67550.t1	AT3G21230	24.689	482	318	14	12	472	40	497	7.66e-18	87.0
MS.gene67550.t1	AT1G20510	23.760	484	299	18	9	458	19	466	7.62e-17	83.6
MS.gene67550.t1	AT2G47240	29.167	216	133	6	267	472	285	490	1.60e-16	83.2
MS.gene67550.t1	AT2G47240	29.167	216	133	6	267	472	344	549	1.79e-16	83.2
MS.gene67550.t1	AT2G47240	29.167	216	133	6	267	472	344	549	1.79e-16	83.2
MS.gene67550.t1	AT2G47240	29.167	216	133	6	267	472	344	549	1.79e-16	83.2
MS.gene67550.t1	AT4G11030	23.062	503	290	21	40	472	77	552	9.77e-14	74.7

Find 98 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAACACTCTTCATTGTATT+TGG	0.117189	5.4:-15213711	None:intergenic
CTGAATTATGTTGATCAAAA+AGG	0.196037	5.4:+15213648	MS.gene67550:CDS
TTGCAAATTGTGAAATTATT+AGG	0.204216	5.4:+15213451	MS.gene67550:CDS
AAAAGATATTATTTATTCTA+AGG	0.210086	5.4:+15213902	MS.gene67550:CDS
TTCCACTTTCTTACAATAAC+TGG	0.223827	5.4:-15213597	None:intergenic
GCACACTATGAACTTCATTT+TGG	0.224185	5.4:+15212820	MS.gene67550:CDS
CTTGGATTTGATGTTAATAT+TGG	0.233128	5.4:+15213549	MS.gene67550:CDS
TGATTATAAACTGCTCCTTT+TGG	0.243384	5.4:-15213186	None:intergenic
AAGAAGTTCATCGTATATTA+AGG	0.246952	5.4:-15213073	None:intergenic
GATCTTGTATTAGGACAATA+AGG	0.256872	5.4:-15213017	None:intergenic
TCATAGTGTGCTGGAGTATT+TGG	0.268936	5.4:-15212808	None:intergenic
ATAGGTGAAATCATGTTTAA+AGG	0.270805	5.4:+15213750	MS.gene67550:CDS
ATAGGATCACATTCATTTCT+AGG	0.276426	5.4:-15213129	None:intergenic
GTTGGCCATGATCTTGTATT+AGG	0.280602	5.4:-15213026	None:intergenic
ACACAGTGGCTGCTCGTTCT+AGG	0.291086	5.4:-15206309	None:intergenic
GGGGTGGCTTGAAATTTCTT+TGG	0.296665	5.4:-15212996	None:intergenic
TCACCAAACACCACACTCTT+AGG	0.297868	5.4:-15214134	None:intergenic
TAATAGTCGCGAGTGAATTA+AGG	0.299417	5.4:-15213221	None:intergenic
GTGTGGTGCACCTACCCTTC+TGG	0.311889	5.4:+15213422	MS.gene67550:CDS
ACACTCACCTTATGAGAAAA+AGG	0.315097	5.4:-15213474	None:intergenic
TCTCAACCTTGCTTCTGCTT+TGG	0.326179	5.4:+15206402	MS.gene67550:CDS
AACTACACATCAGGCTCAAC+TGG	0.349825	5.4:+15213159	MS.gene67550:CDS
AACAACATTAGCATTAATAT+TGG	0.350853	5.4:+15212894	MS.gene67550:CDS
CTTAACAAAGTGGCAGAACT+TGG	0.368442	5.4:+15213531	MS.gene67550:CDS
AGAAATTAATTCAAGTGATT+CGG	0.371842	5.4:-15214691	None:intergenic
TGAGTTTGTGAGTTCATTAG+AGG	0.375347	5.4:+15213926	MS.gene67550:CDS
GGGTGGCTTGAAATTTCTTT+GGG	0.377898	5.4:-15212995	None:intergenic
GAGGTATATCTTTCACTAGT+TGG	0.380173	5.4:-15213044	None:intergenic
TAGGTGAAATCATGTTTAAA+GGG	0.386467	5.4:+15213751	MS.gene67550:CDS
TTTGATGTTAATATTGGTTA+TGG	0.391504	5.4:+15213555	MS.gene67550:CDS
TGATGCAATTCATGTTCATA+AGG	0.393999	5.4:+15213389	MS.gene67550:CDS
TTATAAACAGGTTGCAACTT+TGG	0.394543	5.4:+15212783	MS.gene67550:intron
AAAGGGAATACTTCAATGTC+AGG	0.397558	5.4:+15213768	MS.gene67550:CDS
ACCTTACCAGTTGAATTAAG+AGG	0.398367	5.4:-15214161	None:intergenic
TTTAAACATGATTTCACCTA+TGG	0.398368	5.4:-15213748	None:intergenic
AAGGTGAGTGTCACAGTTGC+TGG	0.399927	5.4:+15213486	MS.gene67550:CDS
ATGTTGTTGCATCTAGTTTG+AGG	0.405861	5.4:-15212879	None:intergenic
GTCAACTTAGGAATTTCTAA+TGG	0.406509	5.4:+15206424	MS.gene67550:CDS
ATACGATGAACTTCTTGAAA+AGG	0.413737	5.4:+15213080	MS.gene67550:CDS
TTCAAATTGAAAGAACCATT+AGG	0.416142	5.4:-15213870	None:intergenic
GATTTGCCTCTTAATTCAAC+TGG	0.421617	5.4:+15214155	MS.gene67550:CDS
ATTTGGTTAATGCCTGCTCT+TGG	0.425029	5.4:+15213318	MS.gene67550:CDS
GCAATGGATGGTGTTTCATT+TGG	0.447289	5.4:+15213301	MS.gene67550:CDS
TGATGATGGTGACGAGTGTT+TGG	0.449095	5.4:+15214010	MS.gene67550:CDS
GAACGAGCAGCCACTGTGTA+TGG	0.449210	5.4:+15206313	MS.gene67550:CDS
ATGAAGAGTGTTCCATATGA+TGG	0.450784	5.4:+15213720	MS.gene67550:CDS
AACATGATCATTGTAGATGA+TGG	0.453182	5.4:-15206347	None:intergenic
GCTTCTGCTTTGGTCAACTT+AGG	0.458586	5.4:+15206412	MS.gene67550:CDS
CTAGGTGTTAGGTTACCTAA+TGG	0.459759	5.4:+15213855	MS.gene67550:CDS
TGGTTAATGCCTGCTCTTGG+TGG	0.459815	5.4:+15213321	MS.gene67550:CDS
TATGGTATGACTGAAACATT+AGG	0.461838	5.4:+15213573	MS.gene67550:CDS
GGACTAGGGACCTAGGTGTT+AGG	0.465511	5.4:+15213844	MS.gene67550:CDS
GTTCCTAAGAGTGTGGTGTT+TGG	0.467445	5.4:+15214131	MS.gene67550:CDS
GTCCAGTTATTGTAAGAAAG+TGG	0.471102	5.4:+15213595	MS.gene67550:CDS
TACGATGAACTTCTTGAAAA+GGG	0.473555	5.4:+15213081	MS.gene67550:CDS
AGATCAATTAGATGCTCATA+TGG	0.476810	5.4:+15214109	MS.gene67550:CDS
AAGGTTGAGACATCTTTCAT+AGG	0.478199	5.4:-15206389	None:intergenic
AAAGATATTATTTATTCTAA+GGG	0.478681	5.4:+15213903	MS.gene67550:CDS
AGGGGTTATTGACTTTATGA+TGG	0.478800	5.4:+15213668	MS.gene67550:CDS
GGGATGGTTGGTATAGGACT+AGG	0.479682	5.4:+15213829	MS.gene67550:CDS
ACAGCTACTTTCAAAACCAT+TGG	0.487308	5.4:-15213972	None:intergenic
ATCTTGTATTAGGACAATAA+GGG	0.496289	5.4:-15213016	None:intergenic
GCAATAATTTCCAGAAGGGT+AGG	0.500605	5.4:-15213432	None:intergenic
GTTTGTGAGTTCATTAGAGG+TGG	0.501370	5.4:+15213929	MS.gene67550:CDS
GATGTGTAGTTCACTGATAT+AGG	0.502126	5.4:-15213147	None:intergenic
GACCTGGTTGAGTGCTCTAA+AGG	0.506891	5.4:+15206256	MS.gene67550:CDS
GGCTCAACTGGAATTCCAAA+AGG	0.522211	5.4:+15213171	MS.gene67550:CDS
GCAATTGTGAAATTGAAAGA+TGG	0.524748	5.4:+15214047	MS.gene67550:CDS
GTTGACATGTTTAGATGCAA+TGG	0.528978	5.4:+15213285	MS.gene67550:CDS
ATATCAGTGAACTACACATC+AGG	0.529465	5.4:+15213150	MS.gene67550:CDS
ATTCAAAATTCTTAACAAAG+TGG	0.541410	5.4:+15213521	MS.gene67550:CDS
GAAATAATAGCACCTGCCAT+TGG	0.541429	5.4:-15212847	None:intergenic
ATTGTCCTAATACAAGATCA+TGG	0.544219	5.4:+15213021	MS.gene67550:CDS
CTCAACCAGGTCTTGTGTCA+TGG	0.545213	5.4:-15206245	None:intergenic
AAATGAAGTTCATAGTGTGC+TGG	0.549199	5.4:-15212817	None:intergenic
AGGAAACTGATCGGTGTCAA+AGG	0.571406	5.4:-15206289	None:intergenic
ATTTGCAATAATTTCCAGAA+GGG	0.582244	5.4:-15213436	None:intergenic
TTTCGATGTGAATCCTATGC+CGG	0.582677	5.4:+15213248	MS.gene67550:CDS
AACCTTTAGAGCACTCAACC+AGG	0.592689	5.4:-15206258	None:intergenic
CGTTACCATGACACAAGACC+TGG	0.596346	5.4:+15206240	None:intergenic
TTCAATTTCACAATTGCACA+AGG	0.596592	5.4:-15214041	None:intergenic
TCGTATATTAAGGTATCTTG+AGG	0.597008	5.4:-15213063	None:intergenic
AAGGGAATACTTCAATGTCA+GGG	0.598415	5.4:+15213769	MS.gene67550:CDS
ATGTTTGTACCACCAAGAGC+AGG	0.604931	5.4:-15213330	None:intergenic
TGGTATAGGACTAGGGACCT+AGG	0.607960	5.4:+15213837	MS.gene67550:CDS
AATTTGCAATAATTTCCAGA+AGG	0.610111	5.4:-15213437	None:intergenic
CATTAGGTAACCTAACACCT+AGG	0.611239	5.4:-15213854	None:intergenic
AGCCACTGTGTATGGTGACA+AGG	0.623725	5.4:+15206321	MS.gene67550:CDS
GTGGGGAGATATGATGATGA+TGG	0.634328	5.4:+15213996	MS.gene67550:CDS
TCTTGTATTAGGACAATAAG+GGG	0.643394	5.4:-15213015	None:intergenic
AGTTGACCAAAGCAGAAGCA+AGG	0.644972	5.4:-15206408	None:intergenic
GGATGGTTGGTATAGGACTA+GGG	0.648496	5.4:+15213830	MS.gene67550:CDS
GCTCGTTCTAGGAAACTGAT+CGG	0.653949	5.4:-15206298	None:intergenic
ACATGTTTAGATGCAATGGA+TGG	0.657007	5.4:+15213289	MS.gene67550:CDS
TGTATTAGGACAATAAGGGG+TGG	0.669864	5.4:-15213012	None:intergenic
CATAAGGTAACACATTTGTG+TGG	0.685677	5.4:+15213405	MS.gene67550:CDS
TCATATGGTTCCTAAGAGTG+TGG	0.716052	5.4:+15214124	MS.gene67550:CDS
AACCTTGTCACCATACACAG+TGG	0.766651	5.4:-15206323	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	15206247	15214703	15206247	ID=MS.gene67550
chr5.4	mRNA	15206247	15214703	15206247	ID=MS.gene67550.t1;Parent=MS.gene67550
chr5.4	exon	15206247	15206453	15206247	ID=MS.gene67550.t1.exon1;Parent=MS.gene67550.t1
chr5.4	CDS	15206247	15206453	15206247	ID=cds.MS.gene67550.t1;Parent=MS.gene67550.t1
chr5.4	exon	15212793	15214176	15212793	ID=MS.gene67550.t1.exon2;Parent=MS.gene67550.t1
chr5.4	CDS	15212793	15214176	15212793	ID=cds.MS.gene67550.t1;Parent=MS.gene67550.t1
chr5.4	exon	15214666	15214703	15214666	ID=MS.gene67550.t1.exon3;Parent=MS.gene67550.t1
chr5.4	CDS	15214666	15214703	15214666	ID=cds.MS.gene67550.t1;Parent=MS.gene67550.t1

Gene Sequence

>MS.gene67550

ATGACACAAGACCTGGTTGAGTGCTCTAAAGGTTCACACATACCTTTGACACCGATCAGTTTCCTAGAACGAGCAGCCACTGTGTATGGTGACAAGGTTTCCATCATCTACAATGATCATGTTAGATTTTCTTGGAGAGAAACCTATGAAAGATGTCTCAACCTTGCTTCTGCTTTGGTCAACTTAGGAATTTCTAATGGTGATATTGTAAGTTCATTTATCTCCCTATGATGACTACATTAATTCGTATACTTTCCTTTGCCCTGCTTTCATTTCATCTAAAGCCAACTATTGTTTGTTCCCTCTTCATGACCAAATATAATGTATCAATGTAATTTAAATTAGAAAATATATATGATCAATGTTTTATATCACTTGTAACAAAATATAAGCACAAAATCAGATCTAAGAAGTCAAATGTTTTTGTCTAAATTTTGGACTAATTACTTTTTAAATATTTTTTGCTTATATTTTGTTAGAGTTTGCCAATATTTTGTTACAGTTTACCAATAGAATTATGTTCTTCAATCGTGCATGAATTAATTAACTGGTTATTCTAATTGGTCCATACAAACTAGTGTTCTATCCATGGTTTTAAATTGGTCATTAAATCATACAATTAGGCTTAAATAGTTAATAAGCTCCACAGGAGGATGATTGAACTTGATTATTGGGTGAAATAATTTTGCCTAAACTTTACTTACCTTACCACTGAACTCCAAATTATGAGATCCCCTTTCTGTGAGTAATGGAGCTTACCACTGAACTCCAAATTATGAGATCCCCTTTCTGTGAGTAATGGAGGATTAACAAAAAAAAAGAAGTTCTTTGAATCGATTACACTAATCAATTTGGCAACCAAGTGACCAGTTTTGCAGTCACTTAATGAAAGTTTTTTAGCACGTAATTAGCAGTTAATTATGGAGAATGCTAAATAGTATTCCAGAGTATTGGTTATACAACCCAAAGAAGTAAGTTTTTATTAAAAAGTGGTATAATTATTACTTAATCAAAAGTAACATCTTATATTTTCAAAACAAAAATTCTATTTTTAAATTACTTAACCAGTGCCCCAAAGACAAAAACACCGATTAACAAGACCCTAATTATAAATATTAAATTTCATATTATAAGGATAATGCTAACCAGTACCCCAATGACATTGGTTAAGCATCCAAAACAAACAAGTTTTCATGAAAGAGTTGTGAGATCATTACTTGGTCAAAAATAACATCTTATATTTCCAAGATAAAATTTCTATTTATAGAATCCTTAACCAATACTCTGAGGGCACTGGTTAGCAAGATCCTAATTATAAATATTAAATTTCACTGAATCAACGGTTGATTATTACAATATCATAATAATGATCAAGCCGATAAACTTTCTTTCTTTTTAATATTAAAAATAAAGTATTCATTTAAATTATATCAAATACAACACAACTTTTATATCGTTAAAAATAAAAAGGATAAATCTGCAAACAAACTCACACCGTTCATATTAAAAATGTAAAATCTAAAAATATGTGTTTACACACACATTGATGACTCGTTCATTATAGTTTCATTCAAAATTCAATTTATATTTGTTATTGTGATTTAAAACATTCAAAAAAGGCTTTTATATATTGCCAAGGTTCTAACTACAGTGTGTATTTTTTATGTATCAGAATCATTTTATGTTAATGTGAATCTTTTTAAACTCTTTTTAATGATAAAAATTTAATCCAAATTTGTAAAATTATATCGTTACGAAAAGGCCATCGAATTCAAAAAAGCCTTTACAAAGTTCATCTCTCTCTAAAAACTCTAACATGTCTCAAACGTGTGATTTTCGAGTCTAAAACTTATCGTTTACCAACTCTAACATGGCTCAAACGTGTGACTATCGAGTCTAAAACTTATTGTTTATCAATAGACCAAGTATTTGGAGTTAGAAGATGATTCATTGGAAGTGAATGATTTCATATTTTTTTTCCTTCAGGATCTGAAATAATTCTATAAGCGCGTAATTATGGAAAATGGGACAGTACCGTGTTCAACACTGTTTATCTATATTCATATATATATTTTTTTTTTTGGTCAAGTCTATATTCATATATTTTAAAATGATTAATTCGCTGTCCTTATTCTTAGACCGAGTTAGGTCATTGACATTTCATTCCATACCAGAATTTACGCCATTAAGAAAAAACACGTTATTAGAATCCCTTAAAAAAACACGTTATTAAAATGGAAAGTCCAGTCTTTGAAAAACTTTATTTTAAGCCATTAGAATGTCTTCAATATGGGAAGTCCACACAGATTTATTATTATTTTTTACTATCCTTTTCTGACATCATTCTGTTTGGAAGAGTATGATCTCACATTACAACACACACCATTCTTGTACATGGTCTATTCCTTTTTTTTCTATTTCTCCTTATTTTCATTCTCTTGATCTGAATTCACTACAGAATTGTACACTATAGTAGATTGGTGTGGAATATGAATCTCTAAGAATGTAAACAAATTAAAAAACTAAGGGTTGTTACGTTAGCAAACTCTTTTTTTTTTTTGTTTTTTTGACAAGTCAATATTTGTTATGATGATGTATCTAGAGTAAATTACTCTATTCCCTCAAAGATGTTTGAATTACATTACCTTTCCTCTTTTGTTAAAATATACACTCCTCTCACCTCTCATTCAAAATATATTAGAAAATATTTCACTGCCCACCCCTAAGAGAAACTTGAAATACATTCTCCTCCCCTCTTATGTTAAAATCTACACTTTAATCTCTCAATAATTTTTTTTATTTCTAATAATCTAGAAAAAAAATAATCTAACACACTTCGGGAAAAAATAGTATTGCAAGTCTATTTTAATATAATCAAAATTTGAATACATATTTTGCAATTTTTTATTCACAATTTCATTAACATATAATTTTAAAGTCTAATAAAAAATATGAAACAAGCCAAGAAAAGCCTAATAAAAAATATGAAACAAGCGAAGATAAAATTAAAATATGTGAAAAATTACAAAATACAATAAATTATGGTTATTTAAAAATTAATTTTATACTCTAAAATATAAAATTAATAGCAAAATATTTAAATTTAATTATCATTGATATTTAAATTATAAAGAAATGAATTTATTTTTTTTAAATGGTGGTTCAAAATTAGTATATATCATAAAAATATTTATTTAAGTTAAAATATATTAAAGTGTAGTGCATATTTAATTATTAAGGGAGAGAGTTGTAATTCAAGCATCTTATAGGGGAAGGGAGTATAAATTTTTCTTTTCAAGGAAGGGGAGTGCATATTTTAACGAGTAAATAGGCAATTTCCCCCCCGAAATTGCAAGGTTTGTCAAATATCCCCCTGTAGTTAACGAAACATCAATTACCCCCCTGAAATTGCATAACGTTAATCAATTTACCCCCTCCGTCAATTTTTCTGTTAACTGTTTACGGTTATGATCAATTACCCCCCTGAAATTTTACACTTATGTGCAAAATGCCCCCTGAACTTGAAAAATTAAAAAAAATTGAAAAAATTCTGGAAAAATGAAAAATCAATAAAAAAAAATTGAAAAAAAAGATTAAAAAATTCTGGAAAATTGAAAAATTAATAAAAATAATCTGAAAAAATATAAAAAATCTGGAAAAATTAAAAAAAAATCTGAAAAAATTAATAAAAATAAAAAATGATTAAAAAAATAAAATAATCTAATAAAATGAAAAATAATGAAAAAATTCTGGAAAATAAAAAATATTTAGATTAGTATATATGTCGAAATTTCGAAATTCTGGAAAAAATGTATTTTTCGAAATTCCGAAATTTTGATTAGTATATATGTCGATCTTAGGTTTTTTCAGTGTATTTTTTCCCTTATTCTCGGTTTATAAATACACAAAAAAGTTCCCGGTTTATAAATACACAAAAATTCCCGGAAAAAATACTAATGAAAATATCGAAATTTTGAAAAATACATAAAAAACCTAAGCTCGGTTTATAAATACACATATTTTTTCCCTTATTCTCGGTTTATAAATACACAAAAATTCCCGGAAATTTTTTGAGAAGGGAGTCCCTAATATGGACCTTAAAAATAAAGGTGCACTTGTAGACCTGTGCAAATGACTTGTGACTGCCGGTTATCCTCAATCAATTTTCTTAATAATTTTAATTTTATTAGTGGCCATGAATAAATTGGCACCAATAACCCTTCACCCTCCCCCATTCCCTTTTTCTTTCACTGAACTCACATTACATTCACATTAAATCTCCACCATAAACTTCATTCATTGGAGCCTCACCCAACGCCACCTCACTCTTCTACAAACACTCATAGGCATCACAGCGGTATGAAGCAGGACTCACTCTTGAGATTTGAGAAAGTTATGGATACAACAAGTGAGGAAATCAATGATTTAACCATCGAACTTGTTAATTGTTATGAAAAGATTTTGAGTTACTGGAAAGAAAAAAATACAACATGAAATAGTGATAACGAGGCTACAAATCCAATTTCAGTAGATTTGTAAAGAAAATCTTTCATGGTTCTTTCTATGATTCAGTCAAAACTCAACGGAGAGGACAAAAGTATATCTAATATTTGATGTTCTTTTTTGTATGATCTCGTGAAGGCGTAGGAATGATTAGGATAAGATTGGTATTTATTATTTGGACAATAAACCTCAGAAATTAATAACTAAATTCAGCAAGCATAACCGGCTGTGGCAGGTCAGTTTGACAGGTCCACCAGTGCACCCTTATTTTTAGGGTCCATATTAGGGGCTTCCTTTTGAGAAATACTAATGAAAATTTCAAAAATTTCCAAAATTTTTCATTAAAATTTCTTTTTTCCAGAATTTCATTAAAATTTCGAAAATTTTTATTAAAATTTCTTTTTTCTAGAATTTCATTAAAATTTTTATTTTTGAGAATTTCCTGAATTTTTATTTTCCAGAATTTCATTAAAATTTAAAATTTCGAAAATTTCATTTTTCCAGAATTTTAATTTTTTTAGAAATTTTGAAATTTTATTTTTTTCCAGAATTTTTTAATCTTTTTTATTTTTTTCAGAATTTTTTCATTATTTTTCATTTTATTAGATTATTTTATTTTTATTAATTTTTCCAGATTTTTTAAATTTTTTCAGATTTTTTTATTTTTATTAATTTTTCCAGATTTTTTTCATTTTGATTAATTTTTCAATTTTCCAGAATTTTTTAATATTTTTTTTCAATTTTTTTATTGATTTTTCATTTTTCCAGAATTTTTTAATTTTTTTTTAATATTTTTCTAGAATTTTTTCAATTTTTTTTAATTTTTCAAGTTCAGGGGGCATTTTGCACATAAGTGTAAAATTTCAGGGGGTAATTGATCATAACCGTAAACAGTTAACAGAAAAATTGACGGAGGGGGTAAATTGATTAACGTTATGCAATTTCAGGGGGGTAATTGATGTTTCGTTAACTACAGGGGGGTATTTGACAAACCTTGCAATTTCGGGGGGGGGGGGGGGGGGGGGGGGGGAAATTGGCTATTTACTCTATTTTAACATAAGAGGGGAGAGGAGTGTAATTCAAACATCTTTGAGTTTTAACATAAGAGGGGAGAGGAGTGTAATTCAAACATCTTTGAGGGAAGGGGAGTGTAACTTACTCTATATAATATCTACATTTGATTACTTTTATTTTTTTTTTGAAAAGATCTACATTTGATTTGTTAACTAACAATTGATACTACTTACTTTATTCTATTATCAAATAGCAACATATCTGTCATCGTGAACTTAGCTCAGTTGGTATGGACAATGCATGATATAATATCTGCAAGGTCCGGGGTTCAAACCCCGGCCACCACCAATAAAAAAATAACAACATATCTATTTTTTTTTAAAAGTATATATCTATCATATATCTTTCTTTTTAAACAACCATATTCATTGCATTTCGGTTAATATAGAATTCAAAATACAAGGAGGAATATAATAAAAAACTTGGCGACAAACAAATAATCAAGATAACCTAGTCAAAACATGAGGCACACCATTAGGTTTTTTTTTTTTTTTTGAGAACCATTAGGTTTTCTATCAAAGTCAATATTTTTTTTATTAGAAAGTCAATATTATTTAAAACTCGCTATATGATCCTCCATTTATCAAATGACTATATAATTTGATGATTTACAAAAGTTTCTAAACAAATGCAACATGAGAATACAAATTAATATAGTGGTCTAGCAAAAACACTAAAATGTAGCTTGTCTAATTTCGGTTTATCCCCTCCTTGTTTTGTACAGTCATATGCCACAAATGTCTAAGAGAAGAATATTTAAATTATAAGGTTGGAATTCCAAATTTTTTAAAGGGGACAAAAATGCATTTAACCTTTTAATTTATGTTTTGATTAATGAATTAACATTGTCATCAACTCTATTTTCCAAATAAATTTACCTAACCAACATGTAATTGCTCATTATAACCGAAAAGAATTTTTTTCCTATACATATGAAGACCTCAATTTTCTTTCTTATATTATAAACAGGTTGCAACTTTGGCACCAAATACTCCAGCACACTATGAACTTCATTTTGGTGTACCAATGGCAGGTGCTATTATTTCTTCACTTAACCTCAAACTAGATGCAACAACATTAGCATTAATATTGGAACAATTAGAGTCTTGCAAAATCATATTTGTAGACTATGAGTTTATTGATTCTGTACTCAAAGCATCAAAAATAATTTCCCAAAGAAATTTCAAGCCACCCCTTATTGTCCTAATACAAGATCATGGCCAACTAGTGAAAGATATACCTCAAGATACCTTAATATACGATGAACTTCTTGAAAAGGGACAAAAAGATTTTAAGATTTCAATGCCTAGAAATGAATGTGATCCTATATCAGTGAACTACACATCAGGCTCAACTGGAATTCCAAAAGGAGCAGTTTATAATCATAGAAGTGTCTACCTTAATTCACTCGCGACTATTACTCGTTTCGATGTGAATCCTATGCCGGTGTTTTTATGGACAGTTGACATGTTTAGATGCAATGGATGGTGTTTCATTTGGTTAATGCCTGCTCTTGGTGGTACAAACATTTGTCTTAGAAATAATTTTTCAGCAAAAGATATCTTTGATGCAATTCATGTTCATAAGGTAACACATTTGTGTGGTGCACCTACCCTTCTGGAAATTATTGCAAATTGTGAAATTATTAGGCCTTTTTCTCATAAGGTGAGTGTCACAGTTGCTGGTATATTACCATCATTCAAAATTCTTAACAAAGTGGCAGAACTTGGATTTGATGTTAATATTGGTTATGGTATGACTGAAACATTAGGTCCAGTTATTGTAAGAAAGTGGAAACAAAACTTTGATGATGATATTACAAAGCTGAATTATGTTGATCAAAAAGGGGTTATTGACTTTATGATGGTTGAAGTTGATGTGAAAGATCCAAATACAATGAAGAGTGTTCCATATGATGGAAAAACCATAGGTGAAATCATGTTTAAAGGGAATACTTCAATGTCAGGGTATCTTAAGAGTTCACAAGTGAGTCATGAAGCTTTTAGGGATGGTTGGTATAGGACTAGGGACCTAGGTGTTAGGTTACCTAATGGTTCTTTCAATTTGAAAGATAGAGCAAAAGATATTATTTATTCTAAGGGTGAGTTTGTGAGTTCATTAGAGGTGGAAGCTGTTTTGTTGAATCATCCAATGGTTTTGAAAGTAGCTGTTGTGGGGAGATATGATGATGATGGTGACGAGTGTTTGGTTGAGTCACCTTGTGCAATTGTGAAATTGAAAGATGGATGTAGTGCTAATGTTGAAGATATTATCAAGTTTTGTGAAGATCAATTAGATGCTCATATGGTTCCTAAGAGTGTGGTGTTTGGTGATTTGCCTCTTAATTCAACTGGTAAGGTACAAAAGTTTGTTATTAGAGAAAATATTAAAAGTATTGGGTGGTGTGACTCAATTAATTAAGGCGGTGGTTTGTAGGATTTGGATTGATTGCATAACAAATTTTACATATTCATGCAGTCCACATACATCGGTGATGGTTGAATCAAAATCGGACAACCCATATATCTAGCTTAATATTTTAGAGTTTAAAAATTTCAAAATTGATTGCTTTATGCACCATTTAATCTCTGATCTAATTATCACCGATATGCAGACTGCATGATTGTGCGAATTTATTACTGCAGCAAAGCCAATAATAAATAAAACAAGGCAAAATGGAAGTTTGTAATGTGCCAAACAAGCATGTAATAATCTTAAATAAACTTATTTGTTGTTTTATTGTAGACACTTGATGTCATTGTTTCCTAAAGACATATACAATCTATATATGTATTATGACTTGTGTTGAATGTTACAATTAATCTGTATTTTGTTTTTCTAGAAAAGTTTGGGAGTGCAAAAATTGGCCGAATCACTTGA

Protein sequence

>MS.gene67550.t1

MTQDLVECSKGSHIPLTPISFLERAATVYGDKVSIIYNDHVRFSWRETYERCLNLASALVNLGISNGDIVATLAPNTPAHYELHFGVPMAGAIISSLNLKLDATTLALILEQLESCKIIFVDYEFIDSVLKASKIISQRNFKPPLIVLIQDHGQLVKDIPQDTLIYDELLEKGQKDFKISMPRNECDPISVNYTSGSTGIPKGAVYNHRSVYLNSLATITRFDVNPMPVFLWTVDMFRCNGWCFIWLMPALGGTNICLRNNFSAKDIFDAIHVHKVTHLCGAPTLLEIIANCEIIRPFSHKVSVTVAGILPSFKILNKVAELGFDVNIGYGMTETLGPVIVRKWKQNFDDDITKLNYVDQKGVIDFMMVEVDVKDPNTMKSVPYDGKTIGEIMFKGNTSMSGYLKSSQVSHEAFRDGWYRTRDLGVRLPNGSFNLKDRAKDIIYSKGEFVSSLEVEAVLLNHPMVLKVAVVGRYDDDGDECLVESPCAIVKLKDGCSANVEDIIKFCEDQLDAHMVPKSVVFGDLPLNSTEKFGSAKIGRIT