Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68205.t1 | RHN71306.1 | 81.7 | 273 | 20 | 1 | 1 | 273 | 6 | 248 | 3.40E-124 | 454.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68205.t1 | Q9FMS6 | 59.5 | 269 | 104 | 3 | 8 | 271 | 9 | 277 | 2.4e-86 | 320.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68205.t1 | A0A396IZW4 | 81.7 | 273 | 20 | 1 | 1 | 273 | 6 | 248 | 2.5e-124 | 454.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene034815 | MS.gene68205 | PPI |
| MS.gene68205 | MS.gene41101 | PPI |
| MS.gene025629 | MS.gene68205 | PPI |
| MS.gene034250 | MS.gene68205 | PPI |
| MS.gene68205 | MS.gene007154 | PPI |
| MS.gene68205 | MS.gene72977 | PPI |
| MS.gene68205 | MS.gene025628 | PPI |
| MS.gene68205 | MS.gene065708 | PPI |
| MS.gene68205 | MS.gene72978 | PPI |
| MS.gene68205 | MS.gene41100 | PPI |
| MS.gene68205 | MS.gene065709 | PPI |
| MS.gene68205 | MS.gene065707 | PPI |
| MS.gene68205 | MS.gene000609 | PPI |
| MS.gene68205 | MS.gene034816 | PPI |
| MS.gene68205 | MS.gene21541 | PPI |
| MS.gene68205 | MS.gene41097 | PPI |
| MS.gene053225 | MS.gene68205 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68205.t1 | AT5G22250 | 58.333 | 276 | 110 | 3 | 1 | 271 | 2 | 277 | 1.84e-111 | 323 |
| MS.gene68205.t1 | AT3G44260 | 54.815 | 270 | 116 | 3 | 6 | 271 | 12 | 279 | 2.70e-102 | 300 |
| MS.gene68205.t1 | AT2G32070 | 46.241 | 266 | 137 | 5 | 9 | 272 | 8 | 269 | 9.73e-79 | 239 |
| MS.gene68205.t1 | AT5G10960 | 44.245 | 278 | 134 | 6 | 4 | 272 | 3 | 268 | 1.94e-76 | 234 |
| MS.gene68205.t1 | AT1G15920 | 44.118 | 272 | 143 | 5 | 5 | 270 | 8 | 276 | 5.80e-76 | 233 |
| MS.gene68205.t1 | AT1G15920 | 44.118 | 272 | 143 | 5 | 5 | 270 | 8 | 276 | 5.80e-76 | 233 |
| MS.gene68205.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.03e-74 | 229 |
| MS.gene68205.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.03e-74 | 229 |
| MS.gene68205.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.31e-74 | 229 |
| MS.gene68205.t1 | AT1G61470 | 37.295 | 244 | 133 | 7 | 12 | 250 | 1 | 229 | 3.23e-38 | 135 |
| MS.gene68205.t1 | AT1G06450 | 33.969 | 262 | 154 | 8 | 12 | 269 | 8 | 254 | 4.53e-38 | 137 |
| MS.gene68205.t1 | AT3G44240 | 36.123 | 227 | 131 | 6 | 26 | 250 | 2 | 216 | 1.24e-35 | 128 |
| MS.gene68205.t1 | AT1G27820 | 35.685 | 241 | 137 | 7 | 15 | 250 | 8 | 235 | 1.65e-34 | 127 |
| MS.gene68205.t1 | AT1G27890 | 36.100 | 241 | 136 | 7 | 15 | 250 | 3 | 230 | 2.93e-34 | 126 |
Find 47 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCATGATACGTGAAGTT+TGG | 0.309470 | 3.1:-92216159 | MS.gene68205:CDS |
| TGTCATCTGATGCGGGGATC+TGG | 0.360753 | 3.1:-92215761 | MS.gene68205:CDS |
| AATCTCATTCGTGGATTTGT+TGG | 0.372739 | 3.1:-92216112 | MS.gene68205:CDS |
| AACTGTATTGTTCGTAAGGT+TGG | 0.390597 | 3.1:+92216014 | None:intergenic |
| AACTATCAGATCCAGCTTGA+TGG | 0.414722 | 3.1:+92215492 | None:intergenic |
| CAAACTTCACGTATCATGAT+TGG | 0.415819 | 3.1:+92216160 | None:intergenic |
| ATCAATGGAATCTTTATTGT+GGG | 0.431524 | 3.1:+92215850 | None:intergenic |
| TCGATGGATACTGAATTTCC+AGG | 0.435207 | 3.1:-92216073 | MS.gene68205:CDS |
| AATCATGATACGTGAAGTTT+GGG | 0.444962 | 3.1:-92216158 | MS.gene68205:CDS |
| AAATAACTGTATTGTTCGTA+AGG | 0.456687 | 3.1:+92216010 | None:intergenic |
| CTAATATTCTCGATGTGGAT+CGG | 0.457901 | 3.1:-92215529 | MS.gene68205:CDS |
| GCTCTTAACATCATTCAGAT+TGG | 0.459139 | 3.1:-92215971 | MS.gene68205:CDS |
| TAATATTCTCGATGTGGATC+GGG | 0.460981 | 3.1:-92215528 | MS.gene68205:CDS |
| CTCGATGTGGATCGGGCTGT+TGG | 0.479593 | 3.1:-92215521 | MS.gene68205:CDS |
| TCACACCATGAGAGGCATTA+CGG | 0.480920 | 3.1:+92215798 | None:intergenic |
| ACTACACGGTGGTCTTGAAC+GGG | 0.485949 | 3.1:-92215555 | MS.gene68205:CDS |
| ATCATGATTGGTTCTGAACT+AGG | 0.486665 | 3.1:+92216172 | None:intergenic |
| GAGGAATTCAATCTCATTCG+TGG | 0.488675 | 3.1:-92216121 | MS.gene68205:CDS |
| AACATACAATTTCATCTCGA+TGG | 0.498778 | 3.1:-92216089 | MS.gene68205:CDS |
| CACTACACGGTGGTCTTGAA+CGG | 0.504975 | 3.1:-92215556 | MS.gene68205:CDS |
| ACTATCAGATCCAGCTTGAT+GGG | 0.512128 | 3.1:+92215493 | None:intergenic |
| TATCAATGGAATCTTTATTG+TGG | 0.521806 | 3.1:+92215849 | None:intergenic |
| GTTGGAAAGTCCCATCAAGC+TGG | 0.524039 | 3.1:-92215503 | MS.gene68205:CDS |
| GTTCGTAAGGTTGGAGATGA+CGG | 0.530754 | 3.1:+92216023 | None:intergenic |
| TTGACAGCGGAGCATATCAA+TGG | 0.541523 | 3.1:+92215835 | None:intergenic |
| ATTGATATGCTCCGCTGTCA+AGG | 0.556742 | 3.1:-92215833 | MS.gene68205:CDS |
| AAGTTATCTCCCGAATAGCT+TGG | 0.560061 | 3.1:-92215657 | MS.gene68205:CDS |
| TTCGTCCGTAATGCCTCTCA+TGG | 0.560305 | 3.1:-92215803 | MS.gene68205:CDS |
| CAATGAATTCACACCATGAG+AGG | 0.573117 | 3.1:+92215790 | None:intergenic |
| CAATGGAATCTTTATTGTGG+GGG | 0.584758 | 3.1:+92215852 | None:intergenic |
| CTCACCTTAACAGATGCAAA+AGG | 0.589044 | 3.1:-92215947 | MS.gene68205:CDS |
| CGCTACTGTAGTGCACTACA+CGG | 0.597011 | 3.1:-92215569 | MS.gene68205:CDS |
| TGACGGTGGTCAACTTTGAG+TGG | 0.600711 | 3.1:+92216040 | None:intergenic |
| TCGTCAACGAAGATGTGACA+TGG | 0.602644 | 3.1:-92215733 | MS.gene68205:CDS |
| TCAATGGAATCTTTATTGTG+GGG | 0.606892 | 3.1:+92215851 | None:intergenic |
| TTGAGTGGATAAATAACACC+TGG | 0.609070 | 3.1:+92216055 | None:intergenic |
| GAGACAAATCATTATGACGA+AGG | 0.645294 | 3.1:-92216200 | None:intergenic |
| GGTTGCTAATATTCTCGATG+TGG | 0.651359 | 3.1:-92215534 | MS.gene68205:CDS |
| GATCTGATAGTTTATTAACA+TGG | 0.652288 | 3.1:-92215481 | MS.gene68205:CDS |
| CGTGTAGTGCACTACAGTAG+CGG | 0.654385 | 3.1:+92215570 | None:intergenic |
| ATGTCACATCTTCGTTGACG+AGG | 0.659231 | 3.1:+92215735 | None:intergenic |
| CGTCAACGAAGATGTGACAT+GGG | 0.670296 | 3.1:-92215732 | MS.gene68205:CDS |
| GATGTTAAGAGCATCAACGT+TGG | 0.677679 | 3.1:+92215982 | None:intergenic |
| CGTAAGGTTGGAGATGACGG+TGG | 0.714506 | 3.1:+92216026 | None:intergenic |
| CGAAGTCAATACCTTGACAG+CGG | 0.743721 | 3.1:+92215822 | None:intergenic |
| TTGGGCACACAATCTGTACG+AGG | 0.755140 | 3.1:-92216140 | MS.gene68205:CDS |
| TACTGTAGTGCACTACACGG+TGG | 0.764520 | 3.1:-92215566 | MS.gene68205:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGTTTTGAGAAAATTATT+TGG | - | chr3.1:92215952-92215971 | MS.gene68205:CDS | 15.0% |
| ! | AAATAACTGTATTGTTCGTA+AGG | + | chr3.1:92215568-92215587 | None:intergenic | 25.0% |
| ! | ATCAATGGAATCTTTATTGT+GGG | + | chr3.1:92215728-92215747 | None:intergenic | 25.0% |
| ! | GATCTGATAGTTTATTAACA+TGG | - | chr3.1:92216094-92216113 | MS.gene68205:CDS | 25.0% |
| ! | TAAAAACATTCGTTGTATCT+GGG | - | chr3.1:92215666-92215685 | MS.gene68205:CDS | 25.0% |
| ! | TATCAATGGAATCTTTATTG+TGG | + | chr3.1:92215729-92215748 | None:intergenic | 25.0% |
| ! | TTAAAAACATTCGTTGTATC+TGG | - | chr3.1:92215665-92215684 | MS.gene68205:CDS | 25.0% |
| !! | AAAAAGTTTTCCAAGCTATT+CGG | + | chr3.1:92215931-92215950 | None:intergenic | 25.0% |
| !! | AAATATGCTGGAATCATTTT+CGG | - | chr3.1:92216168-92216187 | MS.gene68205:CDS | 25.0% |
| !! | AAATGATTCCAGCATATTTT+TGG | + | chr3.1:92216167-92216186 | None:intergenic | 25.0% |
| AACATACAATTTCATCTCGA+TGG | - | chr3.1:92215486-92215505 | MS.gene68205:CDS | 30.0% | |
| AATCATGATACGTGAAGTTT+GGG | - | chr3.1:92215417-92215436 | MS.gene68205:CDS | 30.0% | |
| AATGAAGTCCAAAAATATGC+TGG | - | chr3.1:92216156-92216175 | MS.gene68205:CDS | 30.0% | |
| TCAATGGAATCTTTATTGTG+GGG | + | chr3.1:92215727-92215746 | None:intergenic | 30.0% | |
| ! | AAAAGTTTTCCAAGCTATTC+GGG | + | chr3.1:92215930-92215949 | None:intergenic | 30.0% |
| !! | TGCATTTGATTTTGCATATC+TGG | - | chr3.1:92215879-92215898 | MS.gene68205:CDS | 30.0% |
| !!! | CAACGAATGTTTTTAAAACG+AGG | + | chr3.1:92215661-92215680 | None:intergenic | 30.0% |
| !!! | GAATGTTTTTAAAACGAGGT+AGG | + | chr3.1:92215657-92215676 | None:intergenic | 30.0% |
| AACTGTATTGTTCGTAAGGT+TGG | + | chr3.1:92215564-92215583 | None:intergenic | 35.0% | |
| AATCTCATTCGTGGATTTGT+TGG | - | chr3.1:92215463-92215482 | MS.gene68205:CDS | 35.0% | |
| CAAACTTCACGTATCATGAT+TGG | + | chr3.1:92215418-92215437 | None:intergenic | 35.0% | |
| CAATCATGATACGTGAAGTT+TGG | - | chr3.1:92215416-92215435 | MS.gene68205:CDS | 35.0% | |
| CAATGGAATCTTTATTGTGG+GGG | + | chr3.1:92215726-92215745 | None:intergenic | 35.0% | |
| CTAATATTCTCGATGTGGAT+CGG | - | chr3.1:92216046-92216065 | MS.gene68205:CDS | 35.0% | |
| GCTCTTAACATCATTCAGAT+TGG | - | chr3.1:92215604-92215623 | MS.gene68205:CDS | 35.0% | |
| TAATATTCTCGATGTGGATC+GGG | - | chr3.1:92216047-92216066 | MS.gene68205:CDS | 35.0% | |
| TTGAGTGGATAAATAACACC+TGG | + | chr3.1:92215523-92215542 | None:intergenic | 35.0% | |
| ! | ATGGCAGACTTTTCAGAAAA+TGG | - | chr3.1:92216113-92216132 | MS.gene68205:CDS | 35.0% |
| ! | GTTACCTTTTGCATCTGTTA+AGG | + | chr3.1:92215635-92215654 | None:intergenic | 35.0% |
| ! | TGCATTTTTGTCATCTGATG+CGG | - | chr3.1:92215806-92215825 | MS.gene68205:CDS | 35.0% |
| !! | ATCATGATTGGTTCTGAACT+AGG | + | chr3.1:92215406-92215425 | None:intergenic | 35.0% |
| !! | TGGAATCATTTTCGGATTAG+AGG | - | chr3.1:92216176-92216195 | MS.gene68205:CDS | 35.0% |
| AACTATCAGATCCAGCTTGA+TGG | + | chr3.1:92216086-92216105 | None:intergenic | 40.0% | |
| AAGTTATCTCCCGAATAGCT+TGG | - | chr3.1:92215918-92215937 | MS.gene68205:CDS | 40.0% | |
| ACTATCAGATCCAGCTTGAT+GGG | + | chr3.1:92216085-92216104 | None:intergenic | 40.0% | |
| CAATGAATTCACACCATGAG+AGG | + | chr3.1:92215788-92215807 | None:intergenic | 40.0% | |
| CTCACCTTAACAGATGCAAA+AGG | - | chr3.1:92215628-92215647 | MS.gene68205:CDS | 40.0% | |
| GAGGAATTCAATCTCATTCG+TGG | - | chr3.1:92215454-92215473 | MS.gene68205:CDS | 40.0% | |
| GGTTGCTAATATTCTCGATG+TGG | - | chr3.1:92216041-92216060 | MS.gene68205:CDS | 40.0% | |
| TCGATGGATACTGAATTTCC+AGG | - | chr3.1:92215502-92215521 | MS.gene68205:CDS | 40.0% | |
| ! | CATTTTTGTCATCTGATGCG+GGG | - | chr3.1:92215808-92215827 | MS.gene68205:CDS | 40.0% |
| ! | CTTTTGCATCTGTTAAGGTG+AGG | + | chr3.1:92215630-92215649 | None:intergenic | 40.0% |
| ! | GCATTTTTGTCATCTGATGC+GGG | - | chr3.1:92215807-92215826 | MS.gene68205:CDS | 40.0% |
| !! | GATGTTAAGAGCATCAACGT+TGG | + | chr3.1:92215596-92215615 | None:intergenic | 40.0% |
| ATGTCACATCTTCGTTGACG+AGG | + | chr3.1:92215843-92215862 | None:intergenic | 45.0% | |
| ATTGATATGCTCCGCTGTCA+AGG | - | chr3.1:92215742-92215761 | MS.gene68205:CDS | 45.0% | |
| CGAAGTCAATACCTTGACAG+CGG | + | chr3.1:92215756-92215775 | None:intergenic | 45.0% | |
| CGTCAACGAAGATGTGACAT+GGG | - | chr3.1:92215843-92215862 | MS.gene68205:CDS | 45.0% | |
| GTTCGTAAGGTTGGAGATGA+CGG | + | chr3.1:92215555-92215574 | None:intergenic | 45.0% | |
| TCGTCAACGAAGATGTGACA+TGG | - | chr3.1:92215842-92215861 | MS.gene68205:CDS | 45.0% | |
| TTGACAGCGGAGCATATCAA+TGG | + | chr3.1:92215743-92215762 | None:intergenic | 45.0% | |
| !! | TCACACCATGAGAGGCATTA+CGG | + | chr3.1:92215780-92215799 | None:intergenic | 45.0% |
| CGCTACTGTAGTGCACTACA+CGG | - | chr3.1:92216006-92216025 | MS.gene68205:CDS | 50.0% | |
| CGTGTAGTGCACTACAGTAG+CGG | + | chr3.1:92216008-92216027 | None:intergenic | 50.0% | |
| GTTGGAAAGTCCCATCAAGC+TGG | - | chr3.1:92216072-92216091 | MS.gene68205:CDS | 50.0% | |
| TACTGTAGTGCACTACACGG+TGG | - | chr3.1:92216009-92216028 | MS.gene68205:CDS | 50.0% | |
| TGACGGTGGTCAACTTTGAG+TGG | + | chr3.1:92215538-92215557 | None:intergenic | 50.0% | |
| TTCGTCCGTAATGCCTCTCA+TGG | - | chr3.1:92215772-92215791 | MS.gene68205:CDS | 50.0% | |
| TTGGGCACACAATCTGTACG+AGG | - | chr3.1:92215435-92215454 | MS.gene68205:CDS | 50.0% | |
| ! | ACTACACGGTGGTCTTGAAC+GGG | - | chr3.1:92216020-92216039 | MS.gene68205:CDS | 50.0% |
| ! | CACTACACGGTGGTCTTGAA+CGG | - | chr3.1:92216019-92216038 | MS.gene68205:CDS | 50.0% |
| TGTCATCTGATGCGGGGATC+TGG | - | chr3.1:92215814-92215833 | MS.gene68205:CDS | 55.0% | |
| ! | CGTAAGGTTGGAGATGACGG+TGG | + | chr3.1:92215552-92215571 | None:intergenic | 55.0% |
| CTCGATGTGGATCGGGCTGT+TGG | - | chr3.1:92216054-92216073 | MS.gene68205:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 92215388 | 92216209 | 92215388 | ID=MS.gene68205 |
| chr3.1 | mRNA | 92215388 | 92216209 | 92215388 | ID=MS.gene68205.t1;Parent=MS.gene68205 |
| chr3.1 | exon | 92215388 | 92216209 | 92215388 | ID=MS.gene68205.t1.exon1;Parent=MS.gene68205.t1 |
| chr3.1 | CDS | 92215388 | 92216209 | 92215388 | ID=cds.MS.gene68205.t1;Parent=MS.gene68205.t1 |
| Gene Sequence |
| Protein sequence |
