AlfalfaGEDB | MS.gene68355

M. sativa cultivar XinJiangDaYe / MS.gene68355

KEGG: -
GO: GO:0003674 GO:0003824
PFAM: PF00501.31 PF13193.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68355.t1	RHN54425.1	96.6	530	16	1	1	530	26	553	1.10E-299	1038.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68355.t1	F4HUK6	44.8	531	275	8	9	530	9	530	1.7e-128	461.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68355.t1	G7K007	96.6	530	16	1	1	530	1	528	7.8e-300	1038.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05015	MS.gene68355	0.815153	1.07E-51	-1.69E-46
MS.gene050173	MS.gene68355	0.854389	1.27E-61	-1.69E-46
MS.gene051138	MS.gene68355	0.817796	2.74E-52	-1.69E-46
MS.gene051586	MS.gene68355	0.83019	3.38E-55	-1.69E-46
MS.gene053488	MS.gene68355	0.815727	7.97E-52	-1.69E-46
MS.gene053561	MS.gene68355	0.835413	1.70E-56	-1.69E-46
MS.gene054056	MS.gene68355	0.843355	1.47E-58	-1.69E-46
MS.gene055942	MS.gene68355	0.847104	1.43E-59	-1.69E-46
MS.gene058628	MS.gene68355	0.809315	2.01E-50	-1.69E-46
MS.gene058629	MS.gene68355	0.804684	1.92E-49	-1.69E-46
MS.gene059428	MS.gene68355	0.844949	5.51E-59	-1.69E-46
MS.gene059690	MS.gene68355	0.847096	1.44E-59	-1.69E-46
MS.gene059691	MS.gene68355	0.816977	4.19E-52	-1.69E-46
MS.gene059694	MS.gene68355	0.839121	1.91E-57	-1.69E-46
MS.gene059697	MS.gene68355	0.826523	2.59E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68355.t1	MTR_5g023730	96.604	530	16	1	1	530	1	528	0.0	1050
MS.gene68355.t1	MTR_5g008800	48.030	533	262	9	3	530	2	524	6.15e-173	499
MS.gene68355.t1	MTR_5g008820	46.692	529	267	8	6	530	5	522	8.52e-169	489
MS.gene68355.t1	MTR_8g074040	47.103	535	266	9	3	530	44	568	1.15e-165	483
MS.gene68355.t1	MTR_4g121600	41.941	546	292	10	3	530	5	543	2.34e-143	425
MS.gene68355.t1	MTR_2g044180	40.693	548	300	10	1	530	3	543	1.47e-140	417
MS.gene68355.t1	MTR_8g074120	46.996	466	236	6	70	530	20	479	3.31e-140	415
MS.gene68355.t1	MTR_5g076240	40.556	540	299	11	4	530	2	532	1.10e-136	407
MS.gene68355.t1	MTR_8g074040	45.733	457	233	7	81	530	2	450	1.01e-133	397
MS.gene68355.t1	MTR_3g064420	40.481	541	295	11	4	526	3	534	1.98e-133	400
MS.gene68355.t1	MTR_5g076220	42.884	541	286	12	4	530	2	533	1.77e-129	389
MS.gene68355.t1	MTR_0161s0050	42.751	538	283	11	9	530	7	535	4.49e-125	378
MS.gene68355.t1	MTR_5g076200	42.533	529	281	12	16	530	14	533	6.23e-125	377
MS.gene68355.t1	MTR_2g044180	39.520	458	255	8	89	530	1	452	1.15e-110	338
MS.gene68355.t1	MTR_0803s0020	26.782	519	347	13	16	527	235	727	8.67e-43	164
MS.gene68355.t1	MTR_5g007640	25.926	540	331	20	12	519	67	569	1.36e-32	132
MS.gene68355.t1	MTR_3g460770	25.862	522	327	19	8	506	16	500	2.55e-30	125
MS.gene68355.t1	MTR_8g039720	26.113	494	309	18	43	511	65	527	4.85e-30	124
MS.gene68355.t1	MTR_1g026310	23.214	504	346	12	42	530	67	544	2.11e-29	122
MS.gene68355.t1	MTR_4g077900	25.873	487	312	15	55	524	1	455	2.80e-29	121
MS.gene68355.t1	MTR_2g095510	25.388	516	348	16	16	522	27	514	3.34e-27	115
MS.gene68355.t1	MTR_4g057730	24.858	527	340	17	16	522	27	517	4.24e-27	115
MS.gene68355.t1	MTR_4g005750	25.641	546	339	20	1	522	6	508	3.85e-26	112
MS.gene68355.t1	MTR_1586s0010	24.365	394	275	10	15	396	39	421	5.92e-26	110
MS.gene68355.t1	MTR_2g105570	25.605	496	319	19	43	522	54	515	1.03e-25	111
MS.gene68355.t1	MTR_5g027500	25.595	504	317	18	54	530	80	552	3.63e-25	109
MS.gene68355.t1	MTR_1g063110	24.138	522	355	15	16	522	28	523	1.45e-24	107
MS.gene68355.t1	MTR_4g057730	24.580	476	307	16	16	471	27	470	1.99e-23	103
MS.gene68355.t1	MTR_5g027480	26.600	500	292	24	54	524	79	532	3.92e-23	103
MS.gene68355.t1	MTR_4g128337	23.563	522	339	17	12	511	29	512	4.61e-23	103
MS.gene68355.t1	MTR_1g063120	24.060	532	347	19	16	522	28	527	1.50e-22	101
MS.gene68355.t1	MTR_1569s0010	27.954	347	226	9	193	530	188	519	6.97e-22	99.4
MS.gene68355.t1	MTR_1g090737	24.655	507	320	18	38	524	79	543	3.19e-21	97.8
MS.gene68355.t1	MTR_4g057730	24.183	459	296	16	16	454	27	453	1.01e-19	92.4
MS.gene68355.t1	MTR_3g035130	21.805	532	352	14	21	530	10	499	1.17e-18	89.4
MS.gene68355.t1	MTR_2g030240	23.768	568	332	20	22	532	8	531	2.38e-18	88.6
MS.gene68355.t1	MTR_4g068670	22.131	488	303	17	44	472	79	548	9.23e-18	87.0
MS.gene68355.t1	MTR_3g088880	23.446	354	235	11	184	522	199	531	1.72e-17	85.9
MS.gene68355.t1	MTR_8g099420	23.679	511	296	21	32	472	66	552	1.94e-15	79.7
MS.gene68355.t1	MTR_3g088870	22.689	357	237	11	184	522	198	533	2.15e-15	79.3
MS.gene68355.t1	MTR_1g073180	25.575	391	253	13	153	530	153	518	3.72e-15	78.6
MS.gene68355.t1	MTR_1054s0020	23.673	490	312	18	9	480	261	706	6.91e-15	78.2
MS.gene68355.t1	MTR_5g009360	22.880	507	299	19	35	472	68	551	1.17e-14	77.4
MS.gene68355.t1	MTR_5g009360	24.740	384	208	15	151	472	61	425	3.37e-14	75.5
MS.gene68355.t1	MTR_1g026430	23.948	309	210	8	42	337	67	363	3.59e-14	75.1
MS.gene68355.t1	MTR_1g026470	24.272	309	209	8	42	337	67	363	3.66e-14	74.7
MS.gene68355.t1	MTR_5g009350	24.266	511	287	22	35	472	68	551	5.96e-14	75.1
MS.gene68355.t1	MTR_5g009350	24.266	511	287	22	35	472	68	551	7.22e-14	74.7
MS.gene68355.t1	MTR_1g086600	33.793	145	86	4	329	472	438	573	9.89e-14	74.3
MS.gene68355.t1	MTR_7g109830	27.551	196	130	4	280	472	363	549	1.19e-13	73.9
MS.gene68355.t1	MTR_1g073180	25.131	382	249	12	153	522	175	531	1.49e-13	73.6
MS.gene68355.t1	MTR_5g009350	24.366	513	280	23	35	470	68	549	2.05e-13	73.2
MS.gene68355.t1	MTR_5g009350	24.409	508	276	23	35	465	68	544	3.98e-13	72.4
MS.gene68355.t1	MTR_2g030240	24.250	400	238	14	181	532	55	437	1.88e-12	69.7
MS.gene68355.t1	MTR_1g034360	28.481	158	91	6	322	472	408	550	1.80e-11	67.0
MS.gene68355.t1	MTR_7g084850	25.485	361	221	13	187	520	358	697	3.09e-11	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68355.t1	AT1G20560	46.328	531	267	8	9	530	9	530	2.74e-168	488
MS.gene68355.t1	AT2G17650	46.542	535	258	10	15	530	60	585	3.30e-156	459
MS.gene68355.t1	AT5G16340	41.296	540	293	10	3	530	2	529	5.18e-138	410
MS.gene68355.t1	AT1G76290	42.243	535	288	10	3	530	2	522	1.95e-137	409
MS.gene68355.t1	AT3G16910	41.011	534	287	10	16	530	22	546	2.06e-136	407
MS.gene68355.t1	AT1G66120	41.929	539	291	13	4	529	3	532	3.34e-136	407
MS.gene68355.t1	AT5G16370	40.741	540	296	10	3	530	2	529	5.92e-135	403
MS.gene68355.t1	AT1G20560	44.760	458	236	7	82	530	3	452	9.46e-135	399
MS.gene68355.t1	AT1G75960	41.495	535	288	10	9	530	7	529	2.29e-132	396
MS.gene68355.t1	AT1G65890	38.419	544	309	9	4	528	2	538	9.47e-129	388
MS.gene68355.t1	AT1G65880	39.051	548	300	11	4	528	3	539	3.37e-128	386
MS.gene68355.t1	AT1G21530	39.509	529	299	11	15	530	13	533	8.06e-127	382
MS.gene68355.t1	AT1G21540	40.076	529	297	11	15	530	13	534	1.02e-126	382
MS.gene68355.t1	AT1G21530	39.509	529	297	12	15	530	13	531	1.42e-124	376
MS.gene68355.t1	AT1G77240	38.710	527	302	12	15	530	13	529	2.29e-121	367
MS.gene68355.t1	AT1G68270	35.229	545	295	12	4	528	2	508	1.61e-103	321
MS.gene68355.t1	AT3G21240	25.651	499	323	17	43	524	64	531	1.10e-28	120
MS.gene68355.t1	AT1G65060	25.731	513	338	16	12	511	46	528	1.19e-27	117
MS.gene68355.t1	AT3G21240	26.174	447	286	16	43	472	64	483	1.09e-26	113
MS.gene68355.t1	AT3G21240	26.174	447	286	16	43	472	64	483	1.09e-26	113
MS.gene68355.t1	AT5G63380	28.161	348	225	12	184	522	202	533	2.92e-26	113
MS.gene68355.t1	AT4G05160	25.806	527	331	18	19	522	30	519	1.19e-25	111
MS.gene68355.t1	AT1G20510	25.512	537	354	19	9	528	19	526	1.24e-25	110
MS.gene68355.t1	AT1G51680	26.025	488	318	17	43	513	66	527	1.13e-24	108
MS.gene68355.t1	AT1G51680	26.380	489	316	18	40	511	64	525	1.54e-24	107
MS.gene68355.t1	AT1G20480	24.954	549	350	20	9	530	36	549	6.16e-24	105
MS.gene68355.t1	AT1G20500	26.514	479	297	18	62	522	84	525	6.55e-24	105
MS.gene68355.t1	AT1G51680	26.667	450	290	16	40	472	64	490	3.17e-23	103
MS.gene68355.t1	AT1G20510	25.470	479	316	16	9	471	19	472	1.29e-22	101
MS.gene68355.t1	AT5G38120	25.192	520	331	17	25	524	46	527	2.10e-22	101
MS.gene68355.t1	AT4G19010	23.541	497	309	19	62	530	93	546	3.19e-22	100
MS.gene68355.t1	AT1G62940	23.425	508	329	14	34	524	53	517	7.78e-22	99.4
MS.gene68355.t1	AT5G38120	25.247	507	321	17	25	511	46	514	1.47e-21	98.6
MS.gene68355.t1	AT3G21230	25.373	536	343	18	12	522	40	543	1.85e-21	98.6
MS.gene68355.t1	AT3G21230	25.333	525	335	18	12	511	40	532	3.53e-21	97.4
MS.gene68355.t1	AT5G38120	25.696	467	293	16	25	471	46	478	9.69e-21	95.5
MS.gene68355.t1	AT5G38120	25.696	467	293	16	25	471	46	478	9.69e-21	95.5
MS.gene68355.t1	AT2G47240	24.330	485	298	14	44	472	19	490	1.65e-20	95.5
MS.gene68355.t1	AT2G47240	24.330	485	298	14	44	472	78	549	2.68e-20	95.1
MS.gene68355.t1	AT2G47240	24.330	485	298	14	44	472	78	549	2.68e-20	95.1
MS.gene68355.t1	AT2G47240	24.330	485	298	14	44	472	78	549	2.68e-20	95.1
MS.gene68355.t1	AT1G30520	24.303	502	323	15	22	494	13	486	2.10e-19	92.0
MS.gene68355.t1	AT1G30520	24.303	502	323	15	22	494	13	486	2.10e-19	92.0
MS.gene68355.t1	AT3G48990	24.214	541	315	18	21	524	9	491	1.87e-18	89.0
MS.gene68355.t1	AT3G21230	25.514	486	309	17	12	472	40	497	2.61e-18	88.2
MS.gene68355.t1	AT1G20510	24.524	473	309	18	9	458	19	466	7.84e-17	83.6
MS.gene68355.t1	AT4G11030	22.863	503	291	21	40	472	77	552	9.15e-13	71.2

Find 95 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAACACTCTTCATTGTATT+TGG	0.117189	5.1:-14419141	None:intergenic
CTGAATTATGTTGATCAAAA+AGG	0.196037	5.1:+14419078	MS.gene68355:CDS
AAAAGATATTATTTATTCTA+AGG	0.210086	5.1:+14419332	MS.gene68355:CDS
TTCCACTTTCTTACAATAAC+TGG	0.223827	5.1:-14419027	None:intergenic
GCACACTATGAACTTCATTT+TGG	0.224185	5.1:+14418250	MS.gene68355:CDS
TGATTATAAACTGCTCCTTT+TGG	0.243384	5.1:-14418616	None:intergenic
AAGAAGTTCATCGTATATTA+AGG	0.246952	5.1:-14418503	None:intergenic
TTGCAAACTGTGACATTATT+AGG	0.258350	5.1:+14418881	MS.gene68355:CDS
TCATAGTGTGCTGGAGTATT+TGG	0.268936	5.1:-14418238	None:intergenic
ATAGGTGAAATCATGTTTAA+AGG	0.270805	5.1:+14419180	MS.gene68355:CDS
AGGGTGGCTTGAAATTTCTT+TGG	0.272452	5.1:-14418426	None:intergenic
CTTGGATTTGATGTTAATAT+TGG	0.274603	5.1:+14418979	MS.gene68355:CDS
ATAGGATCACATTCATTTCT+AGG	0.276426	5.1:-14418559	None:intergenic
GTTGGCCATGATCTTGTATT+AGG	0.280602	5.1:-14418456	None:intergenic
ACACAGTGGCTGCTCGTTCT+AGG	0.291086	5.1:-14412584	None:intergenic
TCACCAAACACCACACTCTT+AGG	0.297868	5.1:-14419564	None:intergenic
TAATAGTCGCGAGTGAATTA+AGG	0.299417	5.1:-14418651	None:intergenic
GTGTGGTGCACCTACCCTTC+TGG	0.311889	5.1:+14418852	MS.gene68355:CDS
TCTCAACCTTGCTTCTGCTT+TGG	0.326179	5.1:+14412677	MS.gene68355:CDS
AACTACACATCAGGCTCAAC+TGG	0.349825	5.1:+14418589	MS.gene68355:CDS
AACAACATTAGCATTAATAT+TGG	0.350853	5.1:+14418324	MS.gene68355:CDS
GGGTGGCTTGAAATTTCTTT+GGG	0.360504	5.1:-14418425	None:intergenic
TGAGTTTGTGAGTTCATTAG+AGG	0.375347	5.1:+14419356	MS.gene68355:CDS
GAGGTATATCTTTCACTAGT+TGG	0.380173	5.1:-14418474	None:intergenic
TAGGTGAAATCATGTTTAAA+GGG	0.386467	5.1:+14419181	MS.gene68355:CDS
TGAAGCAATTCATGTTCATA+AGG	0.387735	5.1:+14418819	MS.gene68355:CDS
TTTGATGTTAATATTGGTTA+TGG	0.391504	5.1:+14418985	MS.gene68355:CDS
TTATAAACAGGTTGCAACTT+TGG	0.394543	5.1:+14418213	MS.gene68355:intron
AAAGGGAATACTTCAATGTC+AGG	0.397558	5.1:+14419198	MS.gene68355:CDS
ACCTTACCAGTTGAATTAAG+AGG	0.398367	5.1:-14419591	None:intergenic
TTTAAACATGATTTCACCTA+TGG	0.398368	5.1:-14419178	None:intergenic
AAGGTGAGTGTCACAGTTGC+TGG	0.399927	5.1:+14418916	MS.gene68355:CDS
ATGTTGTTGCATCTAGTTTG+AGG	0.405861	5.1:-14418309	None:intergenic
GTCAACTTAGGAATTTCTAA+TGG	0.406509	5.1:+14412699	MS.gene68355:CDS
ATACGATGAACTTCTTGAAA+AGG	0.413737	5.1:+14418510	MS.gene68355:CDS
TTCAAATTGAAAGAACCATT+AGG	0.416142	5.1:-14419300	None:intergenic
GATTTGCCTCTTAATTCAAC+TGG	0.421617	5.1:+14419585	MS.gene68355:CDS
ATTTGGTTAATGCCTGCTCT+TGG	0.425029	5.1:+14418748	MS.gene68355:CDS
ATCTTGTATTAGGACAATAA+AGG	0.426401	5.1:-14418446	None:intergenic
GCAATGGATGGTGTTTCATT+TGG	0.447289	5.1:+14418731	MS.gene68355:CDS
TGATGATGGTGACGAGTGTT+TGG	0.449095	5.1:+14419440	MS.gene68355:CDS
GAACGAGCAGCCACTGTGTA+TGG	0.449210	5.1:+14412588	MS.gene68355:CDS
ATGAAGAGTGTTCCATATGA+TGG	0.450784	5.1:+14419150	MS.gene68355:CDS
CTTAACAAAGTGGCAGAGCT+TGG	0.451298	5.1:+14418961	MS.gene68355:CDS
AACATGATCATTGTAGATGA+TGG	0.453182	5.1:-14412622	None:intergenic
GCTTCTGCTTTGGTCAACTT+AGG	0.458586	5.1:+14412687	MS.gene68355:CDS
TGGTTAATGCCTGCTCTTGG+TGG	0.459143	5.1:+14418751	MS.gene68355:CDS
CTAGGTGTTAGGTTACCTAA+TGG	0.459759	5.1:+14419285	MS.gene68355:CDS
TATGGTATGACTGAAACATT+AGG	0.461838	5.1:+14419003	MS.gene68355:CDS
GGACTAGGGACCTAGGTGTT+AGG	0.465511	5.1:+14419274	MS.gene68355:CDS
GTTCCTAAGAGTGTGGTGTT+TGG	0.467445	5.1:+14419561	MS.gene68355:CDS
GTCCAGTTATTGTAAGAAAG+TGG	0.471102	5.1:+14419025	MS.gene68355:CDS
TACGATGAACTTCTTGAAAA+GGG	0.473555	5.1:+14418511	MS.gene68355:CDS
AGATCAATTAGATGCTCATA+TGG	0.476810	5.1:+14419539	MS.gene68355:CDS
AAGGTTGAGACATCTTTCAT+AGG	0.478199	5.1:-14412664	None:intergenic
AAAGATATTATTTATTCTAA+GGG	0.478681	5.1:+14419333	MS.gene68355:CDS
AGGGGTTATTGACTTTATGA+TGG	0.478800	5.1:+14419098	MS.gene68355:CDS
GGGATGGTTGGTATAGGACT+AGG	0.479682	5.1:+14419259	MS.gene68355:CDS
ACAGCTACTTTCAAAACCAT+TGG	0.487308	5.1:-14419402	None:intergenic
ATTGTCCTAATACAAGATCA+TGG	0.490140	5.1:+14418451	MS.gene68355:CDS
TCTTGTATTAGGACAATAAA+GGG	0.497609	5.1:-14418445	None:intergenic
GTTTGTGAGTTCATTAGAGG+TGG	0.501370	5.1:+14419359	MS.gene68355:CDS
GATGTGTAGTTCACTGATAT+AGG	0.502126	5.1:-14418577	None:intergenic
GACCTGGTTGAGTGCTCTAA+AGG	0.506891	5.1:+14412531	MS.gene68355:CDS
GCAATAATTTCCAGAAGGGT+AGG	0.508940	5.1:-14418862	None:intergenic
GGCTCAACTGGAATTCCAAA+AGG	0.522211	5.1:+14418601	MS.gene68355:CDS
GCAATTGTGAAATTGAAAGA+TGG	0.524748	5.1:+14419477	MS.gene68355:CDS
GTTGACATGTTTAGATGCAA+TGG	0.528978	5.1:+14418715	MS.gene68355:CDS
ATATCAGTGAACTACACATC+AGG	0.529465	5.1:+14418580	MS.gene68355:CDS
TTTCGATGTGAATCCTATGC+TGG	0.531354	5.1:+14418678	MS.gene68355:CDS
ATGTTTGTGCCACCAAGAGC+AGG	0.540220	5.1:-14418760	None:intergenic
ATTCAAAATTCTTAACAAAG+TGG	0.541410	5.1:+14418951	MS.gene68355:CDS
GAAATAATAGCACCTGCCAT+TGG	0.541429	5.1:-14418277	None:intergenic
CTCAACCAGGTCTTGTGTCA+TGG	0.545213	5.1:-14412520	None:intergenic
AAATGAAGTTCATAGTGTGC+TGG	0.549199	5.1:-14418247	None:intergenic
AGGAAACTGATCGGTGTCAA+AGG	0.571406	5.1:-14412564	None:intergenic
GTTTGCAATAATTTCCAGAA+GGG	0.591302	5.1:-14418866	None:intergenic
AACCTTTAGAGCACTCAACC+AGG	0.592689	5.1:-14412533	None:intergenic
CGTTACCATGACACAAGACC+TGG	0.596346	5.1:+14412515	None:intergenic
TTCAATTTCACAATTGCACA+AGG	0.596592	5.1:-14419471	None:intergenic
TCGTATATTAAGGTATCTTG+AGG	0.597008	5.1:-14418493	None:intergenic
AAGGGAATACTTCAATGTCA+GGG	0.598415	5.1:+14419199	MS.gene68355:CDS
AGTTTGCAATAATTTCCAGA+AGG	0.600004	5.1:-14418867	None:intergenic
TGGTATAGGACTAGGGACCT+AGG	0.607960	5.1:+14419267	MS.gene68355:CDS
CATTAGGTAACCTAACACCT+AGG	0.611239	5.1:-14419284	None:intergenic
AGCCACTGTGTATGGTGACA+AGG	0.623725	5.1:+14412596	MS.gene68355:CDS
GTGGGGAGATATGATGATGA+TGG	0.634328	5.1:+14419426	MS.gene68355:CDS
AGTTGACCAAAGCAGAAGCA+AGG	0.644972	5.1:-14412683	None:intergenic
GGATGGTTGGTATAGGACTA+GGG	0.648496	5.1:+14419260	MS.gene68355:CDS
GCTCGTTCTAGGAAACTGAT+CGG	0.653949	5.1:-14412573	None:intergenic
ACATGTTTAGATGCAATGGA+TGG	0.657007	5.1:+14418719	MS.gene68355:CDS
TGTATTAGGACAATAAAGGG+TGG	0.682560	5.1:-14418442	None:intergenic
CATAAGGTAACACATTTGTG+TGG	0.685677	5.1:+14418835	MS.gene68355:CDS
TCATATGGTTCCTAAGAGTG+TGG	0.716052	5.1:+14419554	MS.gene68355:CDS
AACCTTGTCACCATACACAG+TGG	0.766651	5.1:-14412598	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	14412522	14420014	14412522	ID=MS.gene68355
chr5.1	mRNA	14412522	14420014	14412522	ID=MS.gene68355.t1;Parent=MS.gene68355
chr5.1	exon	14412522	14412728	14412522	ID=MS.gene68355.t1.exon1;Parent=MS.gene68355.t1
chr5.1	CDS	14412522	14412728	14412522	ID=cds.MS.gene68355.t1;Parent=MS.gene68355.t1
chr5.1	exon	14418223	14419606	14418223	ID=MS.gene68355.t1.exon2;Parent=MS.gene68355.t1
chr5.1	CDS	14418223	14419606	14418223	ID=cds.MS.gene68355.t1;Parent=MS.gene68355.t1
chr5.1	exon	14420007	14420014	14420007	ID=MS.gene68355.t1.exon3;Parent=MS.gene68355.t1
chr5.1	CDS	14420007	14420014	14420007	ID=cds.MS.gene68355.t1;Parent=MS.gene68355.t1

Gene Sequence

>MS.gene68355

ATGACACAAGACCTGGTTGAGTGCTCTAAAGGTTCACACATACCTTTGACACCGATCAGTTTCCTAGAACGAGCAGCCACTGTGTATGGTGACAAGGTTTCCATCATCTACAATGATCATGTTAGATTTTCTTGGAGAGAAACCTATGAAAGATGTCTCAACCTTGCTTCTGCTTTGGTCAACTTAGGAATTTCTAATGGTGATATTGTAAGTTCATTTATCTCCCTATCTCCCTATGATGACTACATTAATTCGTATACTTTCCTTTGCCCTGCTTTCATTTCATCTAAAGCCAACTATTGTTTGTTCCCTCTTCATGACCAAATATAATGTATCAATGTAATTTAAATTAGAAAATATATATGATCAATGTTTTATATCACTTGTAACAAAATATAAGCACAAAATCAGATCTAAGAAGTCAAATGTTTTTGTCTAAATTTTGGACTAATTACTTTTTAAATATTTTTTGCTTATATTTTGTTAGAGTTTGCCAATATTTTGTTACAGTTTACCAATAGAATTATGTTCTTCAATCGTGCATGAATTAATTAACTGGTTATTCTAATTGGTCCATACAAACTAGTGTTCTATCCATGGTTTTAAATTGGTCATTAAATCATACAATTAGGCTTAAATAGTTAATAAGCTCCACAGGAGGATGATTGAACTTGATTATTGGGTGAAATAATTTTGCCTAGACTTTACTTACCTTATCACTGAACTCCAAATTATGAGATCCCCTTTCTGTGAGTAATGGAGCTTACCACTGAACTCCAAATTATGAGATCCCCTTTCTGTGAGTAATGGAGGATTAACAAAAAAAAAAAAGTTCTTTGAATCGATTACACTAATCAATTTGGCAACCAAGCGACCAGTTTTGCAGTCACTTAATGAAAGTTTTTTAGCACGTAATTAGCAGTTAATTATGGAGAATGCTAAATAGTATTCCAGAGTATTGGTTATACAACCCAAAGAAGTAAGTTTTTATTAAAAAGTGGTATAATTATTACTTAATTAAAAGTAACATCTTATATTTTCAAAACAAAAATTCTATTTTTAAATTACTTAACCAGTGCCCCAAAGACAAAGACACCGATTAACAAGACCCTAATTATAAATATTAAATTTCATATTATAAGGATAATGCTAACCAGTACCCCAATGACATTGGTTAAGCATCCAAAACAAACAAGTTTTCATGAAAGAGTTGTGAGATCATTACTTGGTCAAAAATAACATCTTATATTTTCAAGATAAAATTTCTATTTATAGAATCCTTAACCAATACTCTGAGGACACTGGTTAGCAAGATCCTAATTATAAATATTAAATTTCACTGAATCAACGGTTGATTATTACAATATCATAATAATGATCAAGCCGATAAACTTTCTTTCTTTTTAATATTAAAAATAAAGTATTCATTTAAATTATATCAAATACAACACAACTTTTATATCGTTAAAAATAAAAAGGATAAATCTGCAAACAAACTCACACCGTTCATATTAAAAATGTAAAATCTAAAAATATGTGTTTACACACACATTGATGACTCGTTCATTATAGTTTCATTCAAAATTCAATTTATATTTGTTATTGTGATTTAAAACATTCAAAAAAGGCTTTTATATATTGCCAAGGTTCTAACTACAGTGTGTATTTTTTATGTATCAGAATCATTTTATGTTAATGTGAATCTTTTTAAACTCTTTTTAATGATAAAAATTTAATCCAAATTTGTAAAATTATATCGTTACGAAAAGGCCATCGAATTCAAAAAAGCCTTTACAAAGTTCATTTCTCTCTAAAAACTCTAACATGTCTCAAACGTGTGATTTTCGAGTCTAAAACTTATCGTTTACCAACTCTAACATGGCTCAAACGTGTGACTATCGAGTCTAAAACTTATCGTTTATCAATAGACCAAGTATTTGGAGTTAGAAGATGATTCATTGGAAGTGAATGATTTCATATTTTTTTTCCTTCAGGATCTGAAATAATTCTATAAGCGCGTAATTATGGAAAATGGGACAGTACATGTATACCGTGTTCAACACTGTTTATCTATATTCATATATATATATTTTTTTTGGTCAAGTCTATATTCATATAATTTTAAAATGATTAATTCGCTGTCCTTGTTCTAAGACCGAGTTAGGTCATTGACATTTCATTCCATACCAGAATTTACGCCATTAAGAAAAAACATGTTATTAGAATCCCTTAAAAAAAACACGTTATTAAAATGGAAAGTCCAGTCTTTGAAAAACTTTATTTTAAGCCATTAGAATGTCTTCAATATGGGAAGTCCACACAGATTTATTATTATTTTTTACTATCCTTTTCTGACATCATTCTGTTTGGAAGAGTATGATCTCACAGGGTCCATTTAACTTGCACCACTAAGAGTTTTGGTCCAACTTGAACCACTTAAATGACCCACTAGTGTAGGTTTTGATCAGGTCATATATACCTTGCACATTAGAAGCCAAACACACGGTAACAAACACCCTCAACATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATATTGTTTAAAAAATAACTTCAATATAAAATTCCACTGTTAACTTAAATATTACAACAAAAAAATATATTTTATTTTAAATTAATTTATATCCCGTCAAATTATTCTTAAATAAACGTAAGCCTTAAATCAATTTTCTAAGTTGTCCTAAAACTCTTTTAACAAATAAACCTTTTATATTCCGTAAAAAAAAATTATATTAATAATGCAGACTTTATGTTCGATTTTAATTTTTCTTTATAATACCATAGTTTACGCATTTTTTTTGTGTGCGCTTAAAGAGTGTGTTTGTTTTATTTTTTTTCAGAAACAAATCTATCTTTTTAATAAAAATAAATAATTTTTTTCATTTTTAAATGTTTGTTAAAGATTTTTAAGAAAATCAGTTTTGATCCTAAAATGAAAAGAAATTAGGATTTGCTTTTTTTTTTCTTTCCAAAATCTCTTTGTTTTTCAAAAACGATATAACACATGAACATGTTAAAAATTATTTTTTTTACAAAGTGATTTTGGTGGATAGTAAATAAACAGATTCCATTTTTTTTTAAAAAAAAGATCCCTAGATTTTTTGTTTAAAAAAAAACTTCAAAAATCTAATTTTTTTTTGTCGGTTTAAAATGAAATCCGCTGTGCATATTGATATTATGTATACAATTGTGTTTTGCAACTATCCTTTCCAAAAATCAATATTGACCCAGACATAATTATCTTGTAAAAAAAAAAGTTATACTAAGTCCCGTTCCATTTTGGTGATTTATCCAACACATAAGTTATTTCACAAAGTGACAAAAATAATCCAATGCAATCAGGCTTACATAAGCCCTCACTTAACCACAACTTGGACATCAAGTTCCAAAATTTCAATCTCAATCGTTTGTTTAAAATATTATTATATCGATGGTCTCTATTCAACTGGTCCAAGTTGGACCCAAACTCTTGGTGGTCCAAATTAAAAGAAACCTGATCTCACATTACAACACACACCATTCTTGGGAAGCACTTGGGTGACATAATGTCACCGTGACATTAAATCTGAGCCACACAACACACATTGTCACACAATATCTGGGCCACACAACACATACAACACATTTGGGCTACACAACACAACACCTTTGTGTGGCCCAGATTTAATGTCACGGTGACATTATGTCACCCAAGTATTTTCCCCATTCTTGTACATGGTCTATTCCTTTTTTTCTATTTCTCCTTATTTTCATTCTCTTCTGAATTCACTACAGAATTGTACACTATAGTAGATTGGTGTGGAATATGAATCTCTAAGAATGTAAACAAATTAAAAAACTAAGGGTTGTTACGTTAGCAAACTCTTTTTTTTTTTTTTTTTTTTTGTTTTTTTGACAAGTCAATATTTGTTATGATGATGTATCTATATATAATAAGAGTAAATTACATTTCTATTCCCTCAAAGATGTTTGAATTACATTACCTTTCCTTTTTTGTTAAAATATACACTCCTCTCACCTCTCATTCAAAATATATTAGAAAATATTTCACTCCCCACCCCTAAGAGAAACTTGAAATACATTCTCCTCCCCTCTTATGTTAAAATCTACACTTTAATCTCTCAATAATTTTTTTTATTTCTAATAATCTAGAAAAAAAATAATCTAACACACTTCGGAAAAAAAATAGTATTGCAGGTCTATTTTAATATAATCAAAATTTGAATACATATTTTGCAATTTTTTATTCACAATTTCATTAACATGTAATTTTAAAGTCTAATAAAAAAATATGAAACAAGCCAAGAAAAGCCTAATAAAAAATATGAAACAAGCGAAGATAAAATTAAAATATGTGAAAAATTATAAAATACAATAAATTATGGTTATTTAAAAATTAATTTTATACTCTAAAATATAAAATTAATAGCAAAATATTTAAATTTAATTATCATTGATATTTAAATTATAAAGAAATGAATTTATTTTTTTTAAATGGTGGTTCAAAATTAGTATATATCATAAAAATATTTATTTAAGTTAAAATATATTAAAGTGTAGTGCATATTTAATTATTAAGGGAGAGGGTTGTAATTCAAGCATCTTATAGGGGAAGGGAGTATAAATTTTTCTTTTCAAGGAAGGGGAGTGCATATTTTAACATAAGAGGGGAGATGAGTGTAATTCAAACATCTTTGAGGGAAGGGGAGTGTAACTTACTCTATATAATATCTACATTTGATTACTTTTATTTTTTTTGAAAAGATCTACATTTGATTTGTTAACTAACAATTGATACTACTTACTTTATTCTATTATCAAATAACAACATATCTGTCATTGTGAACTTAGCTCAGTTGGTATGGACAATGCATGATATAATATATAATATATGCAAGGTCCGGGGTTCAAACCCCGGCCACCACCAATAAAAAAATTGTTCCGGGGTTCAAACCCCGGCCACCACCAATAAAAAAATAACAACATATCTATTTTTTTTAAAAAAGTATATATCTATCATATATCTTTCTTTTTAAACAACCATATTCATTGCATTATCTTTCTTTTTAAACAACCATATTCATTGCATTTCGGTTAATATAGAATTCAAAATACAAGGAGGAATATAATAAAAAACTTGGCGACAAACAAATAATCAAGATAACCTAGTCAAAACATGAAGCACACCATTAGGTTTTCTATCAAAGTCAATATATTTTTTTTATTAGAAAGTCAATATTATATAAAACTAGCTATATGATCCTCCATTTGATTATTTACAAAAGTTTCTAAACAAATGCAACATGAGAATACAAATTAATATAGTGGTCTAGCAAAAACACTAAAATGTAGCTTGTCTAATTTCGGTTTATCCCCTCCCACATCATTTGTTTTGTACGGTCATATGCCACAAATGTCTAAGAGAAGAATATTTAAATTATAAGGTTGGAATTCCAAATTTTTCAAAGGGGACAAAAATGCATTTAACCTTTTAATTTATGTTTTGATTAATGAATTAACATTGTCATCAACTCTATTTTCCAAATAAATTTACCTAACCAACATGTAATTGCTCATTATAACCGAAAAGAATTTTTTTCCTATACATATGAAGACCTCAATTTTCTTTCTTATATTATAAACAGGTTGCAACTTTGGCACCAAATACTCCAGCACACTATGAACTTCATTTTGGTGTACCAATGGCAGGTGCTATTATTTCTTCACTTAACCTCAAACTAGATGCAACAACATTAGCATTAATATTGGAACAGTTAGAGTCTTGCAAAATCATATTTGTAGACTATGAGTTTATTGATTCTGTACTCAAAGCATCAAAAATAATTTCCCAAAGAAATTTCAAGCCACCCTTTATTGTCCTAATACAAGATCATGGCCAACTAGTGAAAGATATACCTCAAGATACCTTAATATACGATGAACTTCTTGAAAAGGGACAAAAAGATTTTAAGATTTCAATGCCTAGAAATGAATGTGATCCTATATCAGTGAACTACACATCAGGCTCAACTGGAATTCCAAAAGGAGCAGTTTATAATCATAGAAGTGTCTACCTTAATTCACTCGCGACTATTACTCGTTTCGATGTGAATCCTATGCTGGTGTTTTTATGGACAGTTGACATGTTTAGATGCAATGGATGGTGTTTCATTTGGTTAATGCCTGCTCTTGGTGGCACAAACATTTGTCTTAGAAATGATTTTTCAGCAAAAGATATTTTTGAAGCAATTCATGTTCATAAGGTAACACATTTGTGTGGTGCACCTACCCTTCTGGAAATTATTGCAAACTGTGACATTATTAGGCATTTTTCTCATAAGGTGAGTGTCACAGTTGCTGGTATATTACCATCATTCAAAATTCTTAACAAAGTGGCAGAGCTTGGATTTGATGTTAATATTGGTTATGGTATGACTGAAACATTAGGTCCAGTTATTGTAAGAAAGTGGAAACAAAACTTTGATGATGATATTACAAAGCTGAATTATGTTGATCAAAAAGGGGTTATTGACTTTATGATGGTTGAAGTTGATGTGAAAGATCCAAATACAATGAAGAGTGTTCCATATGATGGAAAAACCATAGGTGAAATCATGTTTAAAGGGAATACTTCAATGTCAGGGTATCTTAAGAGTTCACAAGTGAGTCATGAAGCTTTTAGGGATGGTTGGTATAGGACTAGGGACCTAGGTGTTAGGTTACCTAATGGTTCTTTCAATTTGAAAGATAGAGCAAAAGATATTATTTATTCTAAGGGTGAGTTTGTGAGTTCATTAGAGGTGGAAGCTGTTTTGTTGAATCATCCAATGGTTTTGAAAGTAGCTGTTGTGGGGAGATATGATGATGATGGTGACGAGTGTTTGGTTGAGTCACCTTGTGCAATTGTGAAATTGAAAGATGGATGTAGTGCTAATGTTGAAGATATTATCAAGTTTTGTGAAGATCAATTAGATGCTCATATGGTTCCTAAGAGTGTGGTGTTTGGTGATTTGCCTCTTAATTCAACTGGTAAGGTACAAAAGTTTGTTATTAGAGAAAATATTAAAAGTATTGGGTGGTGTGACTCAATTAATTAAGGCGTGGTTTGTAGGATTTGGATTGATTGCATAACAAATTTTACATATTCATGCAGTCCACATACATCGGTGATGGTTGAATCAAAATCGGACAACCCATATATCTAGCTTAATATTTTAGAGTTTAAAAATTTCAAAATAGATTGCTTAATGCACCATTTAATCTCTAATCTAATTATCACCGATATGCAGACTGCATGATTGTGCGAATTTATTACTGCAGCAAAGCCAAATCAGTGGTTTGCATGATAATAAATAAAACAAGGCAAAATGGAATGTGCCAAACAAGCATGTAATAATCTTAAATAAACTTATTTGTTGTTTTATTGTAGACACTTGA

Protein sequence

>MS.gene68355.t1

MTQDLVECSKGSHIPLTPISFLERAATVYGDKVSIIYNDHVRFSWRETYERCLNLASALVNLGISNGDIVATLAPNTPAHYELHFGVPMAGAIISSLNLKLDATTLALILEQLESCKIIFVDYEFIDSVLKASKIISQRNFKPPFIVLIQDHGQLVKDIPQDTLIYDELLEKGQKDFKISMPRNECDPISVNYTSGSTGIPKGAVYNHRSVYLNSLATITRFDVNPMLVFLWTVDMFRCNGWCFIWLMPALGGTNICLRNDFSAKDIFEAIHVHKVTHLCGAPTLLEIIANCDIIRHFSHKVSVTVAGILPSFKILNKVAELGFDVNIGYGMTETLGPVIVRKWKQNFDDDITKLNYVDQKGVIDFMMVEVDVKDPNTMKSVPYDGKTIGEIMFKGNTSMSGYLKSSQVSHEAFRDGWYRTRDLGVRLPNGSFNLKDRAKDIIYSKGEFVSSLEVEAVLLNHPMVLKVAVVGRYDDDGDECLVESPCAIVKLKDGCSANVEDIIKFCEDQLDAHMVPKSVVFGDLPLNSTDT