Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68798.t1 | RHN71306.1 | 81.7 | 273 | 20 | 1 | 1 | 273 | 6 | 248 | 3.40E-124 | 454.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68798.t1 | Q9FMS6 | 59.5 | 269 | 104 | 3 | 8 | 271 | 9 | 277 | 2.4e-86 | 320.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68798.t1 | A0A396IZW4 | 81.7 | 273 | 20 | 1 | 1 | 273 | 6 | 248 | 2.5e-124 | 454.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68798 | MS.gene065709 | PPI |
| MS.gene68798 | MS.gene72977 | PPI |
| MS.gene68798 | MS.gene065707 | PPI |
| MS.gene053225 | MS.gene68798 | PPI |
| MS.gene68798 | MS.gene025628 | PPI |
| MS.gene68798 | MS.gene034816 | PPI |
| MS.gene034815 | MS.gene68798 | PPI |
| MS.gene68798 | MS.gene72978 | PPI |
| MS.gene68798 | MS.gene41100 | PPI |
| MS.gene68798 | MS.gene41097 | PPI |
| MS.gene025629 | MS.gene68798 | PPI |
| MS.gene68798 | MS.gene000609 | PPI |
| MS.gene034250 | MS.gene68798 | PPI |
| MS.gene68798 | MS.gene21541 | PPI |
| MS.gene68798 | MS.gene007154 | PPI |
| MS.gene68798 | MS.gene41101 | PPI |
| MS.gene68798 | MS.gene065708 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68798.t1 | AT5G22250 | 58.333 | 276 | 110 | 3 | 1 | 271 | 2 | 277 | 1.84e-111 | 323 |
| MS.gene68798.t1 | AT3G44260 | 54.815 | 270 | 116 | 3 | 6 | 271 | 12 | 279 | 2.70e-102 | 300 |
| MS.gene68798.t1 | AT2G32070 | 46.241 | 266 | 137 | 5 | 9 | 272 | 8 | 269 | 9.73e-79 | 239 |
| MS.gene68798.t1 | AT5G10960 | 44.245 | 278 | 134 | 6 | 4 | 272 | 3 | 268 | 1.94e-76 | 234 |
| MS.gene68798.t1 | AT1G15920 | 44.118 | 272 | 143 | 5 | 5 | 270 | 8 | 276 | 5.80e-76 | 233 |
| MS.gene68798.t1 | AT1G15920 | 44.118 | 272 | 143 | 5 | 5 | 270 | 8 | 276 | 5.80e-76 | 233 |
| MS.gene68798.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.03e-74 | 229 |
| MS.gene68798.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.03e-74 | 229 |
| MS.gene68798.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.31e-74 | 229 |
| MS.gene68798.t1 | AT1G61470 | 37.295 | 244 | 133 | 7 | 12 | 250 | 1 | 229 | 3.23e-38 | 135 |
| MS.gene68798.t1 | AT1G06450 | 33.969 | 262 | 154 | 8 | 12 | 269 | 8 | 254 | 4.53e-38 | 137 |
| MS.gene68798.t1 | AT3G44240 | 36.123 | 227 | 131 | 6 | 26 | 250 | 2 | 216 | 1.24e-35 | 128 |
| MS.gene68798.t1 | AT1G27820 | 35.685 | 241 | 137 | 7 | 15 | 250 | 8 | 235 | 1.65e-34 | 127 |
| MS.gene68798.t1 | AT1G27890 | 36.100 | 241 | 136 | 7 | 15 | 250 | 3 | 230 | 2.93e-34 | 126 |
Find 48 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCATGATACGTGAAGTT+TGG | 0.309470 | 3.2:-92417803 | MS.gene68798:CDS |
| TGTCATCTGATGCGGGGATC+TGG | 0.360753 | 3.2:-92417405 | MS.gene68798:CDS |
| AATCTCATTCGTGGATTTGT+TGG | 0.372739 | 3.2:-92417756 | MS.gene68798:CDS |
| AACTGTATTGTTCGTAAGGT+TGG | 0.390597 | 3.2:+92417658 | None:intergenic |
| AACTATCAGATCCAGCTTGA+TGG | 0.414722 | 3.2:+92417136 | None:intergenic |
| CAAACTTCACGTATCATGAT+TGG | 0.415819 | 3.2:+92417804 | None:intergenic |
| ATCAATGGAATCTTTATTGT+GGG | 0.431524 | 3.2:+92417494 | None:intergenic |
| TAATATTCTCGATGTGGATC+GGG | 0.434798 | 3.2:-92417172 | MS.gene68798:CDS |
| TCGATGGATACTGAATTTCC+AGG | 0.435207 | 3.2:-92417717 | MS.gene68798:CDS |
| AATCATGATACGTGAAGTTT+GGG | 0.444962 | 3.2:-92417802 | MS.gene68798:CDS |
| AAATAACTGTATTGTTCGTA+AGG | 0.456687 | 3.2:+92417654 | None:intergenic |
| GCTCTTAACATCATTCAGAT+TGG | 0.459139 | 3.2:-92417615 | MS.gene68798:CDS |
| CTAATATTCTCGATGTGGAT+CGG | 0.460073 | 3.2:-92417173 | MS.gene68798:CDS |
| CTCGATGTGGATCGGGCCGT+TGG | 0.478690 | 3.2:-92417165 | MS.gene68798:CDS |
| TCACACCATGAGAGGCATTA+CGG | 0.480920 | 3.2:+92417442 | None:intergenic |
| ACTACACGGTGGTCTTGAAC+GGG | 0.485949 | 3.2:-92417199 | MS.gene68798:CDS |
| ATCATGATTGGTTCTGAACT+AGG | 0.486665 | 3.2:+92417816 | None:intergenic |
| GAGGAATTCAATCTCATTCG+TGG | 0.488675 | 3.2:-92417765 | MS.gene68798:CDS |
| AACATACAATTTCATCTCGA+TGG | 0.498778 | 3.2:-92417733 | MS.gene68798:CDS |
| CACTACACGGTGGTCTTGAA+CGG | 0.504975 | 3.2:-92417200 | MS.gene68798:CDS |
| ACTATCAGATCCAGCTTGAT+GGG | 0.512128 | 3.2:+92417137 | None:intergenic |
| TATCAATGGAATCTTTATTG+TGG | 0.521806 | 3.2:+92417493 | None:intergenic |
| GTTCGTAAGGTTGGAGATGA+CGG | 0.530754 | 3.2:+92417667 | None:intergenic |
| GTTGGAAAGTCCCATCAAGC+TGG | 0.534537 | 3.2:-92417147 | MS.gene68798:CDS |
| TTGACAGCGGAGCATATCAA+TGG | 0.541523 | 3.2:+92417479 | None:intergenic |
| ATTGATATGCTCCGCTGTCA+AGG | 0.556742 | 3.2:-92417477 | MS.gene68798:CDS |
| AAGTTATCTCCCGAATAGCT+TGG | 0.560061 | 3.2:-92417301 | MS.gene68798:CDS |
| TTCGTCCGTAATGCCTCTCA+TGG | 0.560305 | 3.2:-92417447 | MS.gene68798:CDS |
| CAATGAATTCACACCATGAG+AGG | 0.573117 | 3.2:+92417434 | None:intergenic |
| CAATGGAATCTTTATTGTGG+GGG | 0.584758 | 3.2:+92417496 | None:intergenic |
| CTCACCTTAACAGATGCAAA+AGG | 0.589044 | 3.2:-92417591 | MS.gene68798:CDS |
| CGCTACTGTAGTGCACTACA+CGG | 0.597011 | 3.2:-92417213 | MS.gene68798:CDS |
| TGACGGTGGTCAACTTTGAG+TGG | 0.600711 | 3.2:+92417684 | None:intergenic |
| TCGTCAACGAAGATGTGACA+TGG | 0.602644 | 3.2:-92417377 | MS.gene68798:CDS |
| TCAATGGAATCTTTATTGTG+GGG | 0.606892 | 3.2:+92417495 | None:intergenic |
| TTGAGTGGATAAATAACACC+TGG | 0.609070 | 3.2:+92417699 | None:intergenic |
| AGCTTGATGGGACTTTCCAA+CGG | 0.618452 | 3.2:+92417149 | None:intergenic |
| GAGACAAATCATTATGACGA+AGG | 0.645294 | 3.2:-92417844 | None:intergenic |
| GGTTGCTAATATTCTCGATG+TGG | 0.651359 | 3.2:-92417178 | MS.gene68798:CDS |
| GATCTGATAGTTTATTAACA+TGG | 0.652288 | 3.2:-92417125 | MS.gene68798:CDS |
| CGTGTAGTGCACTACAGTAG+CGG | 0.654385 | 3.2:+92417214 | None:intergenic |
| ATGTCACATCTTCGTTGACG+AGG | 0.659231 | 3.2:+92417379 | None:intergenic |
| CGTCAACGAAGATGTGACAT+GGG | 0.670296 | 3.2:-92417376 | MS.gene68798:CDS |
| GATGTTAAGAGCATCAACGT+TGG | 0.677679 | 3.2:+92417626 | None:intergenic |
| CGTAAGGTTGGAGATGACGG+TGG | 0.714506 | 3.2:+92417670 | None:intergenic |
| CGAAGTCAATACCTTGACAG+CGG | 0.743721 | 3.2:+92417466 | None:intergenic |
| TTGGGCACACAATCTGTACG+AGG | 0.755140 | 3.2:-92417784 | MS.gene68798:CDS |
| TACTGTAGTGCACTACACGG+TGG | 0.764520 | 3.2:-92417210 | MS.gene68798:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGTTTTGAGAAAATTATT+TGG | - | chr3.2:92417596-92417615 | MS.gene68798:CDS | 15.0% |
| ! | AAATAACTGTATTGTTCGTA+AGG | + | chr3.2:92417212-92417231 | None:intergenic | 25.0% |
| ! | ATCAATGGAATCTTTATTGT+GGG | + | chr3.2:92417372-92417391 | None:intergenic | 25.0% |
| ! | GATCTGATAGTTTATTAACA+TGG | - | chr3.2:92417738-92417757 | MS.gene68798:CDS | 25.0% |
| ! | TAAAAACATTCGTTGTATCT+GGG | - | chr3.2:92417310-92417329 | MS.gene68798:CDS | 25.0% |
| ! | TATCAATGGAATCTTTATTG+TGG | + | chr3.2:92417373-92417392 | None:intergenic | 25.0% |
| ! | TTAAAAACATTCGTTGTATC+TGG | - | chr3.2:92417309-92417328 | MS.gene68798:CDS | 25.0% |
| !! | AAAAAGTTTTCCAAGCTATT+CGG | + | chr3.2:92417575-92417594 | None:intergenic | 25.0% |
| !! | AAATATGCTGGAATCATTTT+CGG | - | chr3.2:92417812-92417831 | MS.gene68798:CDS | 25.0% |
| !! | AAATGATTCCAGCATATTTT+TGG | + | chr3.2:92417811-92417830 | None:intergenic | 25.0% |
| AACATACAATTTCATCTCGA+TGG | - | chr3.2:92417130-92417149 | MS.gene68798:CDS | 30.0% | |
| AATCATGATACGTGAAGTTT+GGG | - | chr3.2:92417061-92417080 | MS.gene68798:CDS | 30.0% | |
| AATGAAGTCCAAAAATATGC+TGG | - | chr3.2:92417800-92417819 | MS.gene68798:CDS | 30.0% | |
| TCAATGGAATCTTTATTGTG+GGG | + | chr3.2:92417371-92417390 | None:intergenic | 30.0% | |
| ! | AAAAGTTTTCCAAGCTATTC+GGG | + | chr3.2:92417574-92417593 | None:intergenic | 30.0% |
| !! | TGCATTTGATTTTGCATATC+TGG | - | chr3.2:92417523-92417542 | MS.gene68798:CDS | 30.0% |
| !!! | CAACGAATGTTTTTAAAACG+AGG | + | chr3.2:92417305-92417324 | None:intergenic | 30.0% |
| !!! | GAATGTTTTTAAAACGAGGT+AGG | + | chr3.2:92417301-92417320 | None:intergenic | 30.0% |
| AACTGTATTGTTCGTAAGGT+TGG | + | chr3.2:92417208-92417227 | None:intergenic | 35.0% | |
| AATCTCATTCGTGGATTTGT+TGG | - | chr3.2:92417107-92417126 | MS.gene68798:CDS | 35.0% | |
| CAAACTTCACGTATCATGAT+TGG | + | chr3.2:92417062-92417081 | None:intergenic | 35.0% | |
| CAATCATGATACGTGAAGTT+TGG | - | chr3.2:92417060-92417079 | MS.gene68798:CDS | 35.0% | |
| CAATGGAATCTTTATTGTGG+GGG | + | chr3.2:92417370-92417389 | None:intergenic | 35.0% | |
| CTAATATTCTCGATGTGGAT+CGG | - | chr3.2:92417690-92417709 | MS.gene68798:CDS | 35.0% | |
| GCTCTTAACATCATTCAGAT+TGG | - | chr3.2:92417248-92417267 | MS.gene68798:CDS | 35.0% | |
| TAATATTCTCGATGTGGATC+GGG | - | chr3.2:92417691-92417710 | MS.gene68798:CDS | 35.0% | |
| TTGAGTGGATAAATAACACC+TGG | + | chr3.2:92417167-92417186 | None:intergenic | 35.0% | |
| ! | ATGGCAGACTTTTCAGAAAA+TGG | - | chr3.2:92417757-92417776 | MS.gene68798:CDS | 35.0% |
| ! | GTTACCTTTTGCATCTGTTA+AGG | + | chr3.2:92417279-92417298 | None:intergenic | 35.0% |
| ! | TGCATTTTTGTCATCTGATG+CGG | - | chr3.2:92417450-92417469 | MS.gene68798:CDS | 35.0% |
| !! | ATCATGATTGGTTCTGAACT+AGG | + | chr3.2:92417050-92417069 | None:intergenic | 35.0% |
| !! | TGGAATCATTTTCGGATTAG+AGG | - | chr3.2:92417820-92417839 | MS.gene68798:CDS | 35.0% |
| AACTATCAGATCCAGCTTGA+TGG | + | chr3.2:92417730-92417749 | None:intergenic | 40.0% | |
| AAGTTATCTCCCGAATAGCT+TGG | - | chr3.2:92417562-92417581 | MS.gene68798:CDS | 40.0% | |
| ACTATCAGATCCAGCTTGAT+GGG | + | chr3.2:92417729-92417748 | None:intergenic | 40.0% | |
| CAATGAATTCACACCATGAG+AGG | + | chr3.2:92417432-92417451 | None:intergenic | 40.0% | |
| CTCACCTTAACAGATGCAAA+AGG | - | chr3.2:92417272-92417291 | MS.gene68798:CDS | 40.0% | |
| GAGGAATTCAATCTCATTCG+TGG | - | chr3.2:92417098-92417117 | MS.gene68798:CDS | 40.0% | |
| GGTTGCTAATATTCTCGATG+TGG | - | chr3.2:92417685-92417704 | MS.gene68798:CDS | 40.0% | |
| TCGATGGATACTGAATTTCC+AGG | - | chr3.2:92417146-92417165 | MS.gene68798:CDS | 40.0% | |
| ! | CATTTTTGTCATCTGATGCG+GGG | - | chr3.2:92417452-92417471 | MS.gene68798:CDS | 40.0% |
| ! | CTTTTGCATCTGTTAAGGTG+AGG | + | chr3.2:92417274-92417293 | None:intergenic | 40.0% |
| ! | GCATTTTTGTCATCTGATGC+GGG | - | chr3.2:92417451-92417470 | MS.gene68798:CDS | 40.0% |
| !! | GATGTTAAGAGCATCAACGT+TGG | + | chr3.2:92417240-92417259 | None:intergenic | 40.0% |
| AGCTTGATGGGACTTTCCAA+CGG | + | chr3.2:92417717-92417736 | None:intergenic | 45.0% | |
| ATGTCACATCTTCGTTGACG+AGG | + | chr3.2:92417487-92417506 | None:intergenic | 45.0% | |
| ATTGATATGCTCCGCTGTCA+AGG | - | chr3.2:92417386-92417405 | MS.gene68798:CDS | 45.0% | |
| CGAAGTCAATACCTTGACAG+CGG | + | chr3.2:92417400-92417419 | None:intergenic | 45.0% | |
| CGTCAACGAAGATGTGACAT+GGG | - | chr3.2:92417487-92417506 | MS.gene68798:CDS | 45.0% | |
| GTTCGTAAGGTTGGAGATGA+CGG | + | chr3.2:92417199-92417218 | None:intergenic | 45.0% | |
| TCGTCAACGAAGATGTGACA+TGG | - | chr3.2:92417486-92417505 | MS.gene68798:CDS | 45.0% | |
| TTGACAGCGGAGCATATCAA+TGG | + | chr3.2:92417387-92417406 | None:intergenic | 45.0% | |
| !! | TCACACCATGAGAGGCATTA+CGG | + | chr3.2:92417424-92417443 | None:intergenic | 45.0% |
| CGCTACTGTAGTGCACTACA+CGG | - | chr3.2:92417650-92417669 | MS.gene68798:CDS | 50.0% | |
| CGTGTAGTGCACTACAGTAG+CGG | + | chr3.2:92417652-92417671 | None:intergenic | 50.0% | |
| GTTGGAAAGTCCCATCAAGC+TGG | - | chr3.2:92417716-92417735 | MS.gene68798:CDS | 50.0% | |
| TACTGTAGTGCACTACACGG+TGG | - | chr3.2:92417653-92417672 | MS.gene68798:CDS | 50.0% | |
| TGACGGTGGTCAACTTTGAG+TGG | + | chr3.2:92417182-92417201 | None:intergenic | 50.0% | |
| TTCGTCCGTAATGCCTCTCA+TGG | - | chr3.2:92417416-92417435 | MS.gene68798:CDS | 50.0% | |
| TTGGGCACACAATCTGTACG+AGG | - | chr3.2:92417079-92417098 | MS.gene68798:CDS | 50.0% | |
| ! | ACTACACGGTGGTCTTGAAC+GGG | - | chr3.2:92417664-92417683 | MS.gene68798:CDS | 50.0% |
| ! | CACTACACGGTGGTCTTGAA+CGG | - | chr3.2:92417663-92417682 | MS.gene68798:CDS | 50.0% |
| TGTCATCTGATGCGGGGATC+TGG | - | chr3.2:92417458-92417477 | MS.gene68798:CDS | 55.0% | |
| ! | CGTAAGGTTGGAGATGACGG+TGG | + | chr3.2:92417196-92417215 | None:intergenic | 55.0% |
| CTCGATGTGGATCGGGCCGT+TGG | - | chr3.2:92417698-92417717 | MS.gene68798:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 92417032 | 92417853 | 92417032 | ID=MS.gene68798 |
| chr3.2 | mRNA | 92417032 | 92417853 | 92417032 | ID=MS.gene68798.t1;Parent=MS.gene68798 |
| chr3.2 | exon | 92417032 | 92417853 | 92417032 | ID=MS.gene68798.t1.exon1;Parent=MS.gene68798.t1 |
| chr3.2 | CDS | 92417032 | 92417853 | 92417032 | ID=cds.MS.gene68798.t1;Parent=MS.gene68798.t1 |
| Gene Sequence |
| Protein sequence |
