Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69769.t1 | RHN69025.1 | 97 | 67 | 2 | 0 | 1 | 67 | 1 | 67 | 1.70E-24 | 121.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69769.t1 | A0A396IY71 | 97.0 | 67 | 2 | 0 | 1 | 67 | 1 | 67 | 1.2e-24 | 121.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene69769 | 0.887652 | 1.18E-72 | -1.69E-46 |
| MS.gene050381 | MS.gene69769 | 0.847909 | 8.58E-60 | -1.69E-46 |
| MS.gene050416 | MS.gene69769 | 0.804217 | 2.40E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene69769 | -0.804368 | 2.23E-49 | -1.69E-46 |
| MS.gene051564 | MS.gene69769 | -0.806842 | 6.76E-50 | -1.69E-46 |
| MS.gene051946 | MS.gene69769 | 0.805871 | 1.08E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene69769 | -0.801291 | 9.65E-49 | -1.69E-46 |
| MS.gene052670 | MS.gene69769 | -0.809044 | 2.30E-50 | -1.69E-46 |
| MS.gene053561 | MS.gene69769 | 0.836585 | 8.59E-57 | -1.69E-46 |
| MS.gene054616 | MS.gene69769 | 0.840322 | 9.32E-58 | -1.69E-46 |
| MS.gene054708 | MS.gene69769 | 0.816897 | 4.36E-52 | -1.69E-46 |
| MS.gene056310 | MS.gene69769 | 0.803128 | 4.04E-49 | -1.69E-46 |
| MS.gene056313 | MS.gene69769 | 0.810713 | 1.01E-50 | -1.69E-46 |
| MS.gene056408 | MS.gene69769 | 0.865047 | 7.84E-65 | -1.69E-46 |
| MS.gene058840 | MS.gene69769 | 0.802607 | 5.18E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene69769 | -0.815432 | 9.27E-52 | -1.69E-46 |
| MS.gene059718 | MS.gene69769 | -0.801011 | 1.10E-48 | -1.69E-46 |
| MS.gene060703 | MS.gene69769 | -0.805663 | 1.20E-49 | -1.69E-46 |
| MS.gene060808 | MS.gene69769 | 0.815377 | 9.54E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTCATCATGGTTGATTC+AGG | 0.196823 | 3.4:-77427523 | MS.gene69769:CDS |
| CTGGCTGAGGTTGGTGAGCT+TGG | 0.290535 | 3.4:-77427544 | MS.gene69769:CDS |
| TTCAGATGGACCCAAGTTGC+TGG | 0.349830 | 3.4:-77427563 | MS.gene69769:CDS |
| GATTCATCATGGTTGATTCA+GGG | 0.429834 | 3.4:-77427522 | MS.gene69769:CDS |
| AGCCCTTGATCTTCTTCGTA+TGG | 0.433702 | 3.4:-77427602 | MS.gene69769:CDS |
| TTAGTAGTAATGGAAGTGTA+AGG | 0.460397 | 3.4:-77427475 | None:intergenic |
| GCCCTTGATCTTCTTCGTAT+GGG | 0.464225 | 3.4:-77427601 | MS.gene69769:CDS |
| ACCAACCTCAGCCAGCAACT+TGG | 0.483517 | 3.4:+77427552 | None:intergenic |
| TTCGTATGGGCTTTGACACA+CGG | 0.492946 | 3.4:-77427588 | MS.gene69769:CDS |
| AGGGGTGGTGTTAGTAGTAA+TGG | 0.506379 | 3.4:-77427485 | MS.gene69769:CDS |
| CCCAAGTTGCTGGCTGAGGT+TGG | 0.527272 | 3.4:-77427553 | MS.gene69769:CDS |
| CCAACCTCAGCCAGCAACTT+GGG | 0.528451 | 3.4:+77427553 | None:intergenic |
| GAAGAAGATCAAGGGCTTCG+CGG | 0.529169 | 3.4:+77427608 | None:intergenic |
| AGCCCATACGAAGAAGATCA+AGG | 0.531159 | 3.4:+77427599 | None:intergenic |
| CACTACTAATTTCCGTGCCA+TGG | 0.531867 | 3.4:+77427660 | None:intergenic |
| AGCAACTTGGGTCCATCTGA+AGG | 0.541645 | 3.4:+77427565 | None:intergenic |
| TGGTGAGCTTGGATTCATCA+TGG | 0.561975 | 3.4:-77427533 | MS.gene69769:CDS |
| TGGACCCAAGTTGCTGGCTG+AGG | 0.570124 | 3.4:-77427557 | MS.gene69769:CDS |
| TTTGACACACGGCCTTCAGA+TGG | 0.574107 | 3.4:-77427577 | MS.gene69769:CDS |
| GGGTAAAGTTGAAGCAGGGG+TGG | 0.597668 | 3.4:-77427500 | MS.gene69769:CDS |
| GCCCATACGAAGAAGATCAA+GGG | 0.605404 | 3.4:+77427600 | None:intergenic |
| TTCATCATGGTTGATTCAGG+GGG | 0.612844 | 3.4:-77427520 | MS.gene69769:CDS |
| ATTCATCATGGTTGATTCAG+GGG | 0.614825 | 3.4:-77427521 | MS.gene69769:CDS |
| TGAATTGTTGTACCATGGCA+CGG | 0.621886 | 3.4:-77427672 | None:intergenic |
| GCCGACGACTTTACTTAACA+CGG | 0.631878 | 3.4:+77427636 | None:intergenic |
| TCCGTGTTAAGTAAAGTCGT+CGG | 0.679295 | 3.4:-77427637 | MS.gene69769:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTCATCATGGTTGATTCAG+GGG | - | chr3.4:77427614-77427633 | MS.gene69769:CDS | 35.0% |
| ! | GATTCATCATGGTTGATTCA+GGG | - | chr3.4:77427613-77427632 | MS.gene69769:CDS | 35.0% |
| TCCGTGTTAAGTAAAGTCGT+CGG | - | chr3.4:77427498-77427517 | MS.gene69769:CDS | 40.0% | |
| ! | GGATTCATCATGGTTGATTC+AGG | - | chr3.4:77427612-77427631 | MS.gene69769:CDS | 40.0% |
| ! | TTCATCATGGTTGATTCAGG+GGG | - | chr3.4:77427615-77427634 | MS.gene69769:CDS | 40.0% |
| AGCCCATACGAAGAAGATCA+AGG | + | chr3.4:77427539-77427558 | None:intergenic | 45.0% | |
| AGCCCTTGATCTTCTTCGTA+TGG | - | chr3.4:77427533-77427552 | MS.gene69769:CDS | 45.0% | |
| GCCCATACGAAGAAGATCAA+GGG | + | chr3.4:77427538-77427557 | None:intergenic | 45.0% | |
| GCCCTTGATCTTCTTCGTAT+GGG | - | chr3.4:77427534-77427553 | MS.gene69769:CDS | 45.0% | |
| GCCGACGACTTTACTTAACA+CGG | + | chr3.4:77427502-77427521 | None:intergenic | 45.0% | |
| TGGTGAGCTTGGATTCATCA+TGG | - | chr3.4:77427602-77427621 | MS.gene69769:CDS | 45.0% | |
| ! | AGGGGTGGTGTTAGTAGTAA+TGG | - | chr3.4:77427650-77427669 | MS.gene69769:CDS | 45.0% |
| ! | TTCGTATGGGCTTTGACACA+CGG | - | chr3.4:77427547-77427566 | MS.gene69769:CDS | 45.0% |
| AGCAACTTGGGTCCATCTGA+AGG | + | chr3.4:77427573-77427592 | None:intergenic | 50.0% | |
| AGGGGGTAAAGTTGAAGCAG+GGG | - | chr3.4:77427632-77427651 | MS.gene69769:CDS | 50.0% | |
| CAGGGGGTAAAGTTGAAGCA+GGG | - | chr3.4:77427631-77427650 | MS.gene69769:CDS | 50.0% | |
| GAAGAAGATCAAGGGCTTCG+CGG | + | chr3.4:77427530-77427549 | None:intergenic | 50.0% | |
| TCAGGGGGTAAAGTTGAAGC+AGG | - | chr3.4:77427630-77427649 | MS.gene69769:CDS | 50.0% | |
| TTCAGATGGACCCAAGTTGC+TGG | - | chr3.4:77427572-77427591 | MS.gene69769:CDS | 50.0% | |
| TTTGACACACGGCCTTCAGA+TGG | - | chr3.4:77427558-77427577 | MS.gene69769:CDS | 50.0% | |
| ACCAACCTCAGCCAGCAACT+TGG | + | chr3.4:77427586-77427605 | None:intergenic | 55.0% | |
| CCAACCTCAGCCAGCAACTT+GGG | + | chr3.4:77427585-77427604 | None:intergenic | 55.0% | |
| GGGTAAAGTTGAAGCAGGGG+TGG | - | chr3.4:77427635-77427654 | MS.gene69769:CDS | 55.0% | |
| ! | CCCAAGTTGCTGGCTGAGGT+TGG | - | chr3.4:77427582-77427601 | MS.gene69769:CDS | 60.0% |
| ! | TGGACCCAAGTTGCTGGCTG+AGG | - | chr3.4:77427578-77427597 | MS.gene69769:CDS | 60.0% |
| !! | CTGGCTGAGGTTGGTGAGCT+TGG | - | chr3.4:77427591-77427610 | MS.gene69769:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 77427477 | 77427680 | 77427477 | ID=MS.gene69769 |
| chr3.4 | mRNA | 77427477 | 77427680 | 77427477 | ID=MS.gene69769.t1;Parent=MS.gene69769 |
| chr3.4 | exon | 77427477 | 77427680 | 77427477 | ID=MS.gene69769.t1.exon1;Parent=MS.gene69769.t1 |
| chr3.4 | CDS | 77427477 | 77427680 | 77427477 | ID=cds.MS.gene69769.t1;Parent=MS.gene69769.t1 |
| Gene Sequence |
| Protein sequence |
