Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70740.t1 | XP_003601378.1 | 97.8 | 535 | 12 | 0 | 1 | 535 | 1 | 535 | 3.60E-271 | 943.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70740.t1 | Q9LVE2 | 75.0 | 536 | 125 | 4 | 1 | 529 | 1 | 534 | 1.6e-203 | 710.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70740.t1 | G7J6P7 | 97.8 | 535 | 12 | 0 | 1 | 535 | 1 | 535 | 2.6e-271 | 943.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045221 | MS.gene70740 | PPI |
| MS.gene70740 | MS.gene015941 | PPI |
| MS.gene007383 | MS.gene70740 | PPI |
| MS.gene70740 | MS.gene051425 | PPI |
| MS.gene70740 | MS.gene021631 | PPI |
| MS.gene70740 | MS.gene22385 | PPI |
| MS.gene035014 | MS.gene70740 | PPI |
| MS.gene014785 | MS.gene70740 | PPI |
| MS.gene70740 | MS.gene75564 | PPI |
| MS.gene70740 | MS.gene020631 | PPI |
| MS.gene25819 | MS.gene70740 | PPI |
| MS.gene47441 | MS.gene70740 | PPI |
| MS.gene044183 | MS.gene70740 | PPI |
| MS.gene71483 | MS.gene70740 | PPI |
| MS.gene072957 | MS.gene70740 | PPI |
| MS.gene79120 | MS.gene70740 | PPI |
| MS.gene007562 | MS.gene70740 | PPI |
| MS.gene029756 | MS.gene70740 | PPI |
| MS.gene70740 | MS.gene067043 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70740.t1 | MTR_3g080070 | 98.505 | 535 | 8 | 0 | 1 | 535 | 1 | 535 | 0.0 | 1094 |
| MS.gene70740.t1 | MTR_6g022720 | 66.095 | 525 | 157 | 4 | 1 | 523 | 1 | 506 | 0.0 | 711 |
| MS.gene70740.t1 | MTR_3g086010 | 64.000 | 525 | 176 | 4 | 1 | 524 | 1 | 513 | 0.0 | 700 |
| MS.gene70740.t1 | MTR_1g062950 | 62.635 | 463 | 172 | 1 | 47 | 509 | 14 | 475 | 0.0 | 585 |
| MS.gene70740.t1 | MTR_1g111570 | 58.058 | 484 | 195 | 3 | 40 | 523 | 13 | 488 | 0.0 | 577 |
| MS.gene70740.t1 | MTR_6g022720 | 67.470 | 415 | 115 | 3 | 1 | 413 | 1 | 397 | 0.0 | 572 |
| MS.gene70740.t1 | MTR_1g011460 | 55.153 | 524 | 200 | 6 | 1 | 523 | 1 | 490 | 0.0 | 567 |
| MS.gene70740.t1 | MTR_3g107980 | 54.198 | 524 | 212 | 5 | 1 | 523 | 1 | 497 | 0.0 | 560 |
| MS.gene70740.t1 | MTR_4g113020 | 51.955 | 537 | 226 | 4 | 1 | 534 | 1 | 508 | 0.0 | 547 |
| MS.gene70740.t1 | MTR_7g102900 | 65.565 | 363 | 125 | 0 | 59 | 421 | 8 | 370 | 2.47e-170 | 486 |
| MS.gene70740.t1 | MTR_4g113020 | 54.545 | 418 | 158 | 4 | 1 | 415 | 1 | 389 | 1.44e-153 | 444 |
| MS.gene70740.t1 | MTR_1g112940 | 41.476 | 393 | 228 | 1 | 117 | 509 | 43 | 433 | 1.21e-118 | 357 |
| MS.gene70740.t1 | MTR_7g102920 | 60.825 | 97 | 38 | 0 | 413 | 509 | 22 | 118 | 1.87e-32 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70740.t1 | AT4G15415 | 70.841 | 535 | 135 | 4 | 1 | 533 | 1 | 516 | 0.0 | 774 |
| MS.gene70740.t1 | AT4G15415 | 70.841 | 535 | 135 | 4 | 1 | 533 | 1 | 516 | 0.0 | 774 |
| MS.gene70740.t1 | AT4G15415 | 70.841 | 535 | 135 | 4 | 1 | 533 | 1 | 516 | 0.0 | 774 |
| MS.gene70740.t1 | AT4G15415 | 70.841 | 535 | 135 | 4 | 1 | 533 | 1 | 516 | 0.0 | 774 |
| MS.gene70740.t1 | AT3G21650 | 71.004 | 538 | 143 | 7 | 1 | 529 | 1 | 534 | 0.0 | 768 |
| MS.gene70740.t1 | AT3G21650 | 71.004 | 538 | 143 | 7 | 1 | 529 | 1 | 534 | 0.0 | 768 |
| MS.gene70740.t1 | AT3G26020 | 65.076 | 524 | 159 | 3 | 1 | 514 | 1 | 510 | 0.0 | 684 |
| MS.gene70740.t1 | AT3G26020 | 65.076 | 524 | 159 | 3 | 1 | 514 | 1 | 510 | 0.0 | 684 |
| MS.gene70740.t1 | AT3G26020 | 65.076 | 524 | 159 | 3 | 1 | 514 | 1 | 510 | 0.0 | 684 |
| MS.gene70740.t1 | AT3G26020 | 65.076 | 524 | 159 | 3 | 1 | 514 | 1 | 510 | 0.0 | 684 |
| MS.gene70740.t1 | AT1G13460 | 70.485 | 454 | 131 | 2 | 59 | 509 | 31 | 484 | 0.0 | 679 |
| MS.gene70740.t1 | AT1G13460 | 70.485 | 454 | 131 | 2 | 59 | 509 | 31 | 484 | 0.0 | 679 |
| MS.gene70740.t1 | AT3G26030 | 58.285 | 513 | 173 | 5 | 1 | 511 | 1 | 474 | 0.0 | 608 |
| MS.gene70740.t1 | AT4G15415 | 71.154 | 416 | 100 | 3 | 1 | 414 | 1 | 398 | 0.0 | 601 |
| MS.gene70740.t1 | AT3G26020 | 55.594 | 572 | 153 | 5 | 1 | 514 | 1 | 529 | 0.0 | 592 |
| MS.gene70740.t1 | AT3G09880 | 67.146 | 417 | 137 | 0 | 93 | 509 | 67 | 483 | 0.0 | 582 |
| MS.gene70740.t1 | AT5G03470 | 57.199 | 507 | 191 | 4 | 1 | 507 | 1 | 481 | 0.0 | 577 |
| MS.gene70740.t1 | AT3G26020 | 55.676 | 555 | 162 | 7 | 1 | 514 | 1 | 512 | 0.0 | 567 |
| MS.gene70740.t1 | AT3G26020 | 55.676 | 555 | 162 | 7 | 1 | 514 | 1 | 512 | 0.0 | 567 |
| MS.gene70740.t1 | AT3G26020 | 55.676 | 555 | 162 | 7 | 1 | 514 | 1 | 512 | 0.0 | 567 |
| MS.gene70740.t1 | AT5G25510 | 54.906 | 530 | 208 | 5 | 1 | 530 | 1 | 499 | 0.0 | 564 |
| MS.gene70740.t1 | AT3G54930 | 57.484 | 461 | 188 | 2 | 51 | 509 | 38 | 492 | 0.0 | 531 |
| MS.gene70740.t1 | AT3G54930 | 57.484 | 461 | 188 | 2 | 51 | 509 | 38 | 492 | 0.0 | 531 |
| MS.gene70740.t1 | AT3G54930 | 56.757 | 370 | 147 | 2 | 47 | 414 | 39 | 397 | 4.76e-143 | 418 |
Find 104 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGAAATGTTGGAGATTT+TGG | 0.059751 | 3.1:+68226531 | MS.gene70740:CDS |
| ACTATTCTTCATCGTATTTA+TGG | 0.088355 | 3.1:+68226418 | MS.gene70740:CDS |
| CCTCACCAGCCTACACTTTC+AGG | 0.182591 | 3.1:+68226870 | MS.gene70740:CDS |
| GTTCCTTAATTTCATCTTCT+TGG | 0.194898 | 3.1:-68233775 | None:intergenic |
| ACGAGGCTTTGCCGAGTTTC+CGG | 0.225248 | 3.1:+68225936 | MS.gene70740:CDS |
| TCTTCAAAATCTTCAAGTTC+TGG | 0.228625 | 3.1:+68225803 | MS.gene70740:CDS |
| TCAAATATTCCCTCTCTCTT+TGG | 0.245719 | 3.1:-68226393 | None:intergenic |
| TTCCATGCTGGTTCCATTGC+AGG | 0.257953 | 3.1:-68226241 | None:intergenic |
| TCCTTTGCACTATTAGTTAC+AGG | 0.259155 | 3.1:-68226748 | None:intergenic |
| GGGTTGGTCAATGGTGGTTC+TGG | 0.262801 | 3.1:+68225731 | MS.gene70740:CDS |
| TTAGCTGATACTGTGATTAA+AGG | 0.280130 | 3.1:+68226709 | MS.gene70740:CDS |
| ATTCCAAGAAGATGAAATTA+AGG | 0.317220 | 3.1:+68233772 | MS.gene70740:CDS |
| CTTCAAAATCTTCAAGTTCT+GGG | 0.320774 | 3.1:+68225804 | MS.gene70740:CDS |
| CTGAACGGGCACTGTTTCTA+TGG | 0.327712 | 3.1:+68233567 | MS.gene70740:CDS |
| GGAATCCATGTGGAAGAAAT+TGG | 0.329641 | 3.1:+68233817 | MS.gene70740:CDS |
| CGAGGCTTTGCCGAGTTTCC+GGG | 0.334520 | 3.1:+68225937 | MS.gene70740:CDS |
| GCCGATAATCTTCCCTGCTT+TGG | 0.336864 | 3.1:+68233640 | MS.gene70740:CDS |
| TGGTGGCGGCAAATTTGTTT+AGG | 0.339562 | 3.1:+68226155 | MS.gene70740:CDS |
| CTATTCTTCATCGTATTTAT+GGG | 0.351219 | 3.1:+68226419 | MS.gene70740:CDS |
| ATCCCAAGTTGGTTTGATAA+TGG | 0.351720 | 3.1:+68225895 | MS.gene70740:CDS |
| ATTGCAGGCTCCTCTTCTTC+AGG | 0.359379 | 3.1:-68226226 | None:intergenic |
| AGAAGCTGAATTAGATTTCA+AGG | 0.385056 | 3.1:-68225783 | None:intergenic |
| TCCAAAGCAGGGAAGATTAT+CGG | 0.387741 | 3.1:-68233641 | None:intergenic |
| TTCATTGAACTTTGAATTCA+CGG | 0.389836 | 3.1:-68226107 | None:intergenic |
| TGGATTTGCTTTGCCTTTGA+AGG | 0.397088 | 3.1:+68226567 | MS.gene70740:CDS |
| CGAAGTTCTCTATAGGTTTG+TGG | 0.401536 | 3.1:+68226282 | MS.gene70740:CDS |
| GCAAGCTTTGCATCCGTCTC+TGG | 0.404470 | 3.1:-68226310 | None:intergenic |
| ATTAGATTTCAAGGTGTCAT+TGG | 0.410207 | 3.1:-68225774 | None:intergenic |
| TTGTGTACGAAGTTCTCTAT+AGG | 0.410620 | 3.1:+68226275 | MS.gene70740:CDS |
| TCGTATTTATGGGAAGTTCA+TGG | 0.411443 | 3.1:+68226429 | MS.gene70740:CDS |
| AAGAAATCAGCTCCTGCAAT+TGG | 0.415691 | 3.1:+68225872 | MS.gene70740:CDS |
| CAAATTTGCCGCCACCATCT+TGG | 0.426987 | 3.1:-68226149 | None:intergenic |
| GTTGGTTTGATAATGGCTAA+TGG | 0.428848 | 3.1:+68225902 | MS.gene70740:CDS |
| CTGAAATTCAGCTGCTTGAG+TGG | 0.440484 | 3.1:-68226809 | None:intergenic |
| CCAACTTGGGATCCAATTGC+AGG | 0.455386 | 3.1:-68225884 | None:intergenic |
| CAGGAGCTGATTTCTTAGCT+TGG | 0.457303 | 3.1:-68225865 | None:intergenic |
| AATCTTCAAGTTCTGGGAAT+GGG | 0.461006 | 3.1:+68225810 | MS.gene70740:CDS |
| ATGGCTACAGGTAGCTGAAC+GGG | 0.461747 | 3.1:+68233553 | MS.gene70740:intron |
| CCTGCAATTGGATCCCAAGT+TGG | 0.482538 | 3.1:+68225884 | MS.gene70740:CDS |
| CTAGAAACATCCCGGAAACT+CGG | 0.489064 | 3.1:-68225947 | None:intergenic |
| ACGAGAGAAGCAAGAGAAGC+GGG | 0.492255 | 3.1:+68233796 | MS.gene70740:CDS |
| TTGATAATGGCTAATGGTAA+TGG | 0.493661 | 3.1:+68225908 | MS.gene70740:CDS |
| CACACTTTGGCTTGTGAAGT+GGG | 0.495213 | 3.1:-68226624 | None:intergenic |
| TTCTCTTTAACATGCTTGCT+AGG | 0.496798 | 3.1:-68226037 | None:intergenic |
| TAGCCATTATCAAACCAACT+TGG | 0.499232 | 3.1:-68225898 | None:intergenic |
| AAATCTTCAAGTTCTGGGAA+TGG | 0.502305 | 3.1:+68225809 | MS.gene70740:CDS |
| GTGGGATCAATGCACGGGTA+AGG | 0.507555 | 3.1:-68226606 | None:intergenic |
| TTTCCGGGATGTTTCTAGCT+CGG | 0.507794 | 3.1:+68225952 | MS.gene70740:CDS |
| ATCTTCCAATTTCTTCCACA+TGG | 0.518321 | 3.1:-68233822 | None:intergenic |
| AGCTTATGCTCTTCCTTCAA+AGG | 0.523049 | 3.1:-68226580 | None:intergenic |
| AGCATATGATCCTGAAGAAG+AGG | 0.524548 | 3.1:+68226216 | MS.gene70740:CDS |
| CCACGGAATTGCAGAAATGT+TGG | 0.527762 | 3.1:+68226522 | MS.gene70740:CDS |
| ACACACTTTGGCTTGTGAAG+TGG | 0.530715 | 3.1:-68226625 | None:intergenic |
| TCACATTCAGAGTCAAACTC+TGG | 0.532668 | 3.1:-68233697 | None:intergenic |
| GGTGATGGAGGGTTGGTCAA+TGG | 0.532690 | 3.1:+68225722 | MS.gene70740:CDS |
| GCAAAGGAGGTAATGTTCCT+TGG | 0.533135 | 3.1:+68226763 | MS.gene70740:CDS |
| GAATTCAAAGTTCAATGAAA+CGG | 0.537784 | 3.1:+68226111 | MS.gene70740:CDS |
| TGATACATAGAGACACACTT+TGG | 0.538632 | 3.1:-68226637 | None:intergenic |
| AGCCATTATCAAACCAACTT+GGG | 0.538921 | 3.1:-68225897 | None:intergenic |
| CCTGAAAGTGTAGGCTGGTG+AGG | 0.541063 | 3.1:-68226870 | None:intergenic |
| GTGTTCAAATCATGATGGTA+AGG | 0.542812 | 3.1:+68226186 | MS.gene70740:CDS |
| AACTGCAGCAACGGCAAGAG+AGG | 0.543678 | 3.1:-68233903 | None:intergenic |
| GAGTCAAACTCTGGACAGCT+TGG | 0.546137 | 3.1:-68233688 | None:intergenic |
| ACCAGCATGGAATCATCTAC+AGG | 0.548291 | 3.1:+68226252 | MS.gene70740:CDS |
| ATGAAGCAGTTCTTGCCTCA+AGG | 0.551323 | 3.1:+68233873 | MS.gene70740:CDS |
| AACGAGAGAAGCAAGAGAAG+CGG | 0.557982 | 3.1:+68233795 | MS.gene70740:CDS |
| TCTGGACAGCTTGGTTCCAG+TGG | 0.564294 | 3.1:-68233679 | None:intergenic |
| GATATGATCAAACAGATACT+TGG | 0.565210 | 3.1:+68225650 | None:intergenic |
| ACCTGTAGATGATTCCATGC+TGG | 0.572614 | 3.1:-68226253 | None:intergenic |
| GTGAAGTGGGATCAATGCAC+GGG | 0.574083 | 3.1:-68226611 | None:intergenic |
| TGAAGAAGAGGAGCCTGCAA+TGG | 0.576982 | 3.1:+68226228 | MS.gene70740:CDS |
| TGTTCAGCACCTGAAAGTGT+AGG | 0.578280 | 3.1:-68226879 | None:intergenic |
| GTTTGTGGCTTCACCAGAGA+CGG | 0.579326 | 3.1:+68226297 | MS.gene70740:CDS |
| TAGCAAGCATGTTAAAGAGA+AGG | 0.582818 | 3.1:+68226039 | MS.gene70740:CDS |
| TGCTTCATTGCTTATAGACG+TGG | 0.582925 | 3.1:-68233858 | None:intergenic |
| TTTCCGAGCTAGAAACATCC+CGG | 0.586817 | 3.1:-68225955 | None:intergenic |
| GATGGAGGGTTGGTCAATGG+TGG | 0.588118 | 3.1:+68225725 | MS.gene70740:CDS |
| GTTTCATTGAACTGCAGCAA+CGG | 0.591067 | 3.1:-68233912 | None:intergenic |
| GCTTGACCTCTTTGACTCTG+AGG | 0.591109 | 3.1:+68226369 | MS.gene70740:CDS |
| GCCTGTAACTAATAGTGCAA+AGG | 0.595510 | 3.1:+68226747 | MS.gene70740:CDS |
| AATGTTCCTTGGTGAGCTTG+AGG | 0.596570 | 3.1:+68226774 | MS.gene70740:CDS |
| TGTAACTAATAGTGCAAAGG+AGG | 0.600085 | 3.1:+68226750 | MS.gene70740:CDS |
| AATGCAAGAGATTACCAAGA+TGG | 0.600914 | 3.1:+68226135 | MS.gene70740:CDS |
| TGTGAAGTGGGATCAATGCA+CGG | 0.601606 | 3.1:-68226612 | None:intergenic |
| GAATTTCAGAAATGTGCGGT+GGG | 0.601924 | 3.1:+68226823 | MS.gene70740:CDS |
| CTCTGAGGACCAAAGAGAGA+GGG | 0.604563 | 3.1:+68226384 | MS.gene70740:CDS |
| GTGGAAGAAATTGGAAGATG+TGG | 0.612850 | 3.1:+68233826 | MS.gene70740:CDS |
| AGAGATTACCAAGATGGTGG+CGG | 0.614821 | 3.1:+68226141 | MS.gene70740:CDS |
| TGAATTTCAGAAATGTGCGG+TGG | 0.623403 | 3.1:+68226822 | MS.gene70740:CDS |
| TTTGGTCCTCAGAGTCAAAG+AGG | 0.623928 | 3.1:-68226375 | None:intergenic |
| AGCCTGCAATGGAACCAGCA+TGG | 0.627088 | 3.1:+68226239 | MS.gene70740:CDS |
| TATGGCTACAGGTAGCTGAA+CGG | 0.628011 | 3.1:+68233552 | MS.gene70740:intron |
| CCAACATTTCTGCAATTCCG+TGG | 0.630868 | 3.1:-68226522 | None:intergenic |
| CAGCACCTGAAAGTGTAGGC+TGG | 0.633497 | 3.1:-68226875 | None:intergenic |
| CTACCGTGTTCAAATCATGA+TGG | 0.634397 | 3.1:+68226181 | MS.gene70740:CDS |
| ACTCTGAGGACCAAAGAGAG+AGG | 0.648939 | 3.1:+68226383 | MS.gene70740:CDS |
| TTAAAGAGAAGGATGTCAAG+AGG | 0.651885 | 3.1:+68226050 | MS.gene70740:CDS |
| GCAAGAGATTACCAAGATGG+TGG | 0.652024 | 3.1:+68226138 | MS.gene70740:CDS |
| TAATGGTAATGGAGTTTACG+AGG | 0.679671 | 3.1:+68225919 | MS.gene70740:CDS |
| AGCTGAATTTCAGAAATGTG+CGG | 0.685966 | 3.1:+68226819 | MS.gene70740:CDS |
| AAACTTCCTCAAGCTCACCA+AGG | 0.693184 | 3.1:-68226780 | None:intergenic |
| TTACCATCATGATTTGAACA+CGG | 0.713565 | 3.1:-68226184 | None:intergenic |
| AAGAGAAGCGGGAATCCATG+TGG | 0.736377 | 3.1:+68233807 | MS.gene70740:CDS |
| AAGAGAGGAAGCAAACCTTG+AGG | 0.738771 | 3.1:-68233888 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 68225653 | 68233931 | 68225653 | ID=MS.gene70740 |
| chr3.1 | mRNA | 68225653 | 68233931 | 68225653 | ID=MS.gene70740.t1;Parent=MS.gene70740 |
| chr3.1 | exon | 68225653 | 68226891 | 68225653 | ID=MS.gene70740.t1.exon1;Parent=MS.gene70740.t1 |
| chr3.1 | CDS | 68225653 | 68226891 | 68225653 | ID=cds.MS.gene70740.t1;Parent=MS.gene70740.t1 |
| chr3.1 | exon | 68233563 | 68233931 | 68233563 | ID=MS.gene70740.t1.exon2;Parent=MS.gene70740.t1 |
| chr3.1 | CDS | 68233563 | 68233931 | 68233563 | ID=cds.MS.gene70740.t1;Parent=MS.gene70740.t1 |
| Gene Sequence |
| Protein sequence |
