Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72156.t1 | XP_024629047.1 | 97.4 | 272 | 7 | 0 | 1 | 272 | 1 | 272 | 6.00E-153 | 550.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72156.t1 | Q9LXM2 | 63.4 | 265 | 92 | 3 | 9 | 270 | 17 | 279 | 1.0e-92 | 341.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72156.t1 | I3S8F5 | 97.4 | 272 | 7 | 0 | 1 | 272 | 1 | 272 | 4.3e-153 | 550.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72156 | MS.gene41097 | PPI |
| MS.gene72977 | MS.gene72156 | PPI |
| MS.gene065709 | MS.gene72156 | PPI |
| MS.gene41100 | MS.gene72156 | PPI |
| MS.gene72156 | MS.gene007154 | PPI |
| MS.gene72156 | MS.gene41101 | PPI |
| MS.gene000609 | MS.gene72156 | PPI |
| MS.gene72156 | MS.gene025628 | PPI |
| MS.gene065708 | MS.gene72156 | PPI |
| MS.gene053225 | MS.gene72156 | PPI |
| MS.gene034816 | MS.gene72156 | PPI |
| MS.gene72978 | MS.gene72156 | PPI |
| MS.gene72156 | MS.gene065707 | PPI |
| MS.gene034815 | MS.gene72156 | PPI |
| MS.gene72156 | MS.gene21541 | PPI |
| MS.gene025629 | MS.gene72156 | PPI |
| MS.gene034250 | MS.gene72156 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72156.t1 | AT3G44260 | 63.396 | 265 | 92 | 3 | 9 | 270 | 17 | 279 | 1.21e-119 | 343 |
| MS.gene72156.t1 | AT5G22250 | 64.662 | 266 | 90 | 3 | 9 | 270 | 12 | 277 | 1.91e-119 | 343 |
| MS.gene72156.t1 | AT2G32070 | 52.239 | 268 | 123 | 5 | 4 | 270 | 5 | 268 | 9.13e-95 | 280 |
| MS.gene72156.t1 | AT5G10960 | 52.060 | 267 | 124 | 4 | 4 | 270 | 5 | 267 | 8.93e-94 | 278 |
| MS.gene72156.t1 | AT1G80780 | 49.438 | 267 | 131 | 4 | 4 | 270 | 5 | 267 | 4.56e-90 | 268 |
| MS.gene72156.t1 | AT1G80780 | 49.438 | 267 | 131 | 4 | 4 | 270 | 5 | 267 | 4.56e-90 | 268 |
| MS.gene72156.t1 | AT1G80780 | 49.438 | 267 | 131 | 4 | 4 | 270 | 5 | 267 | 8.66e-90 | 268 |
| MS.gene72156.t1 | AT1G15920 | 48.889 | 270 | 130 | 5 | 5 | 269 | 10 | 276 | 4.81e-83 | 251 |
| MS.gene72156.t1 | AT1G15920 | 48.889 | 270 | 130 | 5 | 5 | 269 | 10 | 276 | 4.81e-83 | 251 |
| MS.gene72156.t1 | AT1G06450 | 35.273 | 275 | 150 | 10 | 1 | 268 | 1 | 254 | 1.06e-40 | 144 |
| MS.gene72156.t1 | AT1G61470 | 37.037 | 243 | 130 | 7 | 13 | 249 | 4 | 229 | 4.05e-38 | 135 |
| MS.gene72156.t1 | AT1G27820 | 35.510 | 245 | 143 | 5 | 7 | 248 | 2 | 234 | 1.31e-33 | 124 |
| MS.gene72156.t1 | AT3G44240 | 35.065 | 231 | 135 | 7 | 24 | 252 | 2 | 219 | 5.20e-33 | 121 |
| MS.gene72156.t1 | AT1G27890 | 35.146 | 239 | 140 | 5 | 13 | 248 | 3 | 229 | 4.85e-32 | 120 |
Find 61 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCACAGTGCTTATGATTT+TGG | 0.076417 | 4.1:-6095894 | MS.gene72156:CDS |
| AACGGTAACCTTCCTCATTT+TGG | 0.104000 | 4.1:-6096122 | MS.gene72156:CDS |
| AAACACGCCGGGGTCTTATT+TGG | 0.170808 | 4.1:-6095597 | MS.gene72156:CDS |
| CAATCATCATCCGTCAAGTT+TGG | 0.286714 | 4.1:-6096352 | MS.gene72156:CDS |
| GCTGAATTGATGCTCAGTTC+TGG | 0.292756 | 4.1:-6095951 | MS.gene72156:CDS |
| TCTTCAACAAATCAGTTGTT+TGG | 0.295934 | 4.1:-6095923 | MS.gene72156:CDS |
| GGACAAATAACTGCTACATA+TGG | 0.328351 | 4.1:-6096100 | MS.gene72156:CDS |
| CTTAACGTGGGTCGGGTTGC+TGG | 0.360050 | 4.1:-6095711 | MS.gene72156:CDS |
| GAATTTGCCTAACCGCTTAG+AGG | 0.364570 | 4.1:-6095844 | MS.gene72156:CDS |
| AGATGACGGCGGTCAACTTT+GGG | 0.366558 | 4.1:+6096230 | None:intergenic |
| CTTCAACAAATCAGTTGTTT+GGG | 0.376564 | 4.1:-6095922 | MS.gene72156:CDS |
| AGCGGTTAGGCAAATTCCTT+CGG | 0.390068 | 4.1:+6095850 | None:intergenic |
| AATCATCATCCGTCAAGTTT+GGG | 0.414340 | 4.1:-6096351 | MS.gene72156:CDS |
| AGTTTGGGCATCTAATTTAG+AGG | 0.418481 | 4.1:-6096336 | MS.gene72156:CDS |
| AAGATGACGGCGGTCAACTT+TGG | 0.418710 | 4.1:+6096229 | None:intergenic |
| TTACCTTCAAGTAACGATAA+TGG | 0.427255 | 4.1:+6096195 | None:intergenic |
| GCGGTTAGGCAAATTCCTTC+GGG | 0.436779 | 4.1:+6095851 | None:intergenic |
| TCCATGGACACTGAATTTCC+TGG | 0.438101 | 4.1:-6096266 | MS.gene72156:CDS |
| CAAACTTGACGGATGATGAT+TGG | 0.450788 | 4.1:+6096353 | None:intergenic |
| AGAGCATGTCAATTGAATCT+TGG | 0.452953 | 4.1:+6096033 | None:intergenic |
| AATTAGATGCCCAAACTTGA+CGG | 0.464834 | 4.1:+6096342 | None:intergenic |
| GACAAATAACTGCTACATAT+GGG | 0.475547 | 4.1:-6096099 | MS.gene72156:CDS |
| CAATTGAATCTTGGTTGTAA+AGG | 0.483444 | 4.1:+6096042 | None:intergenic |
| GCTCTTAAACTTATCCAAGT+TGG | 0.489286 | 4.1:-6096164 | MS.gene72156:CDS |
| TACTTATTTCATGAATAATG+AGG | 0.494803 | 4.1:-6095625 | MS.gene72156:CDS |
| TGCGCTTGAAGTCAATCCCT+TGG | 0.495963 | 4.1:+6096006 | None:intergenic |
| AGACCATTATCGTTACTTGA+AGG | 0.507691 | 4.1:-6096198 | MS.gene72156:CDS |
| TACAACACTTAACGTGGGTC+GGG | 0.508105 | 4.1:-6095718 | MS.gene72156:CDS |
| GATATTGGTTGATAACTTGA+CGG | 0.511970 | 4.1:+6096297 | None:intergenic |
| CCATGGAGATGAAAGGATAT+TGG | 0.513144 | 4.1:+6096282 | None:intergenic |
| CAGTTATTTGTCCCAAAATG+AGG | 0.514189 | 4.1:+6096110 | None:intergenic |
| CCAATATCCTTTCATCTCCA+TGG | 0.518224 | 4.1:-6096282 | MS.gene72156:CDS |
| ACCAGGAAATTCAGTGTCCA+TGG | 0.528331 | 4.1:+6096265 | None:intergenic |
| GCATGCATTTAAGAAAATGA+TGG | 0.528476 | 4.1:-6095649 | MS.gene72156:CDS |
| CTACAACACTTAACGTGGGT+CGG | 0.533742 | 4.1:-6095719 | MS.gene72156:CDS |
| TTCAAGCGCAATTTATGTCA+TGG | 0.539718 | 4.1:-6095993 | MS.gene72156:CDS |
| CAGAGAGGGTGATACCAACT+TGG | 0.540291 | 4.1:+6096150 | None:intergenic |
| ATGATATGAAACACATGATG+AGG | 0.540345 | 4.1:-6095779 | MS.gene72156:CDS |
| GCTGGAAAGTCTCATCAAGC+TGG | 0.542113 | 4.1:-6095693 | MS.gene72156:CDS |
| AAGAAGTCCTCTAAGCGGTT+AGG | 0.544312 | 4.1:+6095837 | None:intergenic |
| GTTGGTATCACCCTCTCTGA+TGG | 0.573766 | 4.1:-6096146 | MS.gene72156:CDS |
| ATTCAAAATGAACCAGCTGA+AGG | 0.587598 | 4.1:-6096381 | None:intergenic |
| GCGCAATTTATGTCATGGTG+TGG | 0.588168 | 4.1:-6095988 | MS.gene72156:CDS |
| TATTTGTCCCAAAATGAGGA+AGG | 0.593755 | 4.1:+6096114 | None:intergenic |
| CAAGTAACGATAATGGTCTG+AGG | 0.595300 | 4.1:+6096202 | None:intergenic |
| AAGTAACGATAATGGTCTGA+GGG | 0.597261 | 4.1:+6096203 | None:intergenic |
| TTGACATGCTCTGTCGCCAA+GGG | 0.598045 | 4.1:-6096022 | MS.gene72156:CDS |
| ATTGACATGCTCTGTCGCCA+AGG | 0.601388 | 4.1:-6096023 | MS.gene72156:CDS |
| TGGTGAAGATCTTGACCCGA+AGG | 0.604460 | 4.1:-6095866 | MS.gene72156:CDS |
| GGTCTGAGGGTTTAAGATGA+CGG | 0.605445 | 4.1:+6096216 | None:intergenic |
| TTCTAATCCAAATAAGACCC+CGG | 0.610017 | 4.1:+6095590 | None:intergenic |
| AGTCGCTACAACACTTAACG+TGG | 0.625698 | 4.1:-6095724 | MS.gene72156:CDS |
| ATCACCCTCTCTGATGGTAA+CGG | 0.635418 | 4.1:-6096140 | MS.gene72156:CDS |
| TCAGTGTCCATGGAGATGAA+AGG | 0.650663 | 4.1:+6096275 | None:intergenic |
| GATCAGATAGTTTGTTGACG+TGG | 0.660381 | 4.1:-6095671 | MS.gene72156:CDS |
| TTGGGTGCATAAATCACACC+AGG | 0.679753 | 4.1:+6096248 | None:intergenic |
| TATTTAAGAAGTCCTCTAAG+CGG | 0.683422 | 4.1:+6095832 | None:intergenic |
| GTTACCGTTACCATCAGAGA+GGG | 0.698499 | 4.1:+6096136 | None:intergenic |
| GTCGCTACAACACTTAACGT+GGG | 0.701411 | 4.1:-6095723 | MS.gene72156:CDS |
| GGTTACCGTTACCATCAGAG+AGG | 0.730463 | 4.1:+6096135 | None:intergenic |
| CTGAGGGTTTAAGATGACGG+CGG | 0.732503 | 4.1:+6096219 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACTTATTTCATGAATAATG+AGG | - | chr4.1:6096327-6096346 | MS.gene72156:CDS | 20.0% |
| ATGATATGAAACACATGATG+AGG | - | chr4.1:6096173-6096192 | MS.gene72156:CDS | 30.0% | |
| CAATTGAATCTTGGTTGTAA+AGG | + | chr4.1:6095913-6095932 | None:intergenic | 30.0% | |
| CTTCAACAAATCAGTTGTTT+GGG | - | chr4.1:6096030-6096049 | MS.gene72156:CDS | 30.0% | |
| GACAAATAACTGCTACATAT+GGG | - | chr4.1:6095853-6095872 | MS.gene72156:CDS | 30.0% | |
| GCATGCATTTAAGAAAATGA+TGG | - | chr4.1:6096303-6096322 | MS.gene72156:CDS | 30.0% | |
| TATTTAAGAAGTCCTCTAAG+CGG | + | chr4.1:6096123-6096142 | None:intergenic | 30.0% | |
| TCTTCAACAAATCAGTTGTT+TGG | - | chr4.1:6096029-6096048 | MS.gene72156:CDS | 30.0% | |
| TTACCTTCAAGTAACGATAA+TGG | + | chr4.1:6095760-6095779 | None:intergenic | 30.0% | |
| ! | TTCACAGTGCTTATGATTTT+GGG | - | chr4.1:6096059-6096078 | MS.gene72156:CDS | 30.0% |
| ! | TTTCACAGTGCTTATGATTT+TGG | - | chr4.1:6096058-6096077 | MS.gene72156:CDS | 30.0% |
| !! | GATATTGGTTGATAACTTGA+CGG | + | chr4.1:6095658-6095677 | None:intergenic | 30.0% |
| !! | TGCTTATGATTTTGGGTATT+TGG | - | chr4.1:6096066-6096085 | MS.gene72156:CDS | 30.0% |
| AATCATCATCCGTCAAGTTT+GGG | - | chr4.1:6095601-6095620 | MS.gene72156:CDS | 35.0% | |
| AATTAGATGCCCAAACTTGA+CGG | + | chr4.1:6095613-6095632 | None:intergenic | 35.0% | |
| AGACCATTATCGTTACTTGA+AGG | - | chr4.1:6095754-6095773 | MS.gene72156:CDS | 35.0% | |
| AGAGCATGTCAATTGAATCT+TGG | + | chr4.1:6095922-6095941 | None:intergenic | 35.0% | |
| AGTTTGGGCATCTAATTTAG+AGG | - | chr4.1:6095616-6095635 | MS.gene72156:CDS | 35.0% | |
| CAGTTATTTGTCCCAAAATG+AGG | + | chr4.1:6095845-6095864 | None:intergenic | 35.0% | |
| GCTCTTAAACTTATCCAAGT+TGG | - | chr4.1:6095788-6095807 | MS.gene72156:CDS | 35.0% | |
| GGACAAATAACTGCTACATA+TGG | - | chr4.1:6095852-6095871 | MS.gene72156:CDS | 35.0% | |
| TATTTGTCCCAAAATGAGGA+AGG | + | chr4.1:6095841-6095860 | None:intergenic | 35.0% | |
| TTCAAGCGCAATTTATGTCA+TGG | - | chr4.1:6095959-6095978 | MS.gene72156:CDS | 35.0% | |
| TTCTAATCCAAATAAGACCC+CGG | + | chr4.1:6096365-6096384 | None:intergenic | 35.0% | |
| ! | AAGTAACGATAATGGTCTGA+GGG | + | chr4.1:6095752-6095771 | None:intergenic | 35.0% |
| CAATCATCATCCGTCAAGTT+TGG | - | chr4.1:6095600-6095619 | MS.gene72156:CDS | 40.0% | |
| CCAATATCCTTTCATCTCCA+TGG | - | chr4.1:6095670-6095689 | MS.gene72156:CDS | 40.0% | |
| CCATGGAGATGAAAGGATAT+TGG | + | chr4.1:6095673-6095692 | None:intergenic | 40.0% | |
| ! | AACGGTAACCTTCCTCATTT+TGG | - | chr4.1:6095830-6095849 | MS.gene72156:CDS | 40.0% |
| ! | ACGGTAACCTTCCTCATTTT+GGG | - | chr4.1:6095831-6095850 | MS.gene72156:CDS | 40.0% |
| ! | AGGTTTTGCAACGCTTTGTA+CGG | - | chr4.1:6096193-6096212 | MS.gene72156:CDS | 40.0% |
| ! | CAAACTTGACGGATGATGAT+TGG | + | chr4.1:6095602-6095621 | None:intergenic | 40.0% |
| ! | CAAGTAACGATAATGGTCTG+AGG | + | chr4.1:6095753-6095772 | None:intergenic | 40.0% |
| !! | GATCAGATAGTTTGTTGACG+TGG | - | chr4.1:6096281-6096300 | MS.gene72156:CDS | 40.0% |
| AAGAAGTCCTCTAAGCGGTT+AGG | + | chr4.1:6096118-6096137 | None:intergenic | 45.0% | |
| AATGAGGCTCAAAAACACGC+CGG | - | chr4.1:6096343-6096362 | MS.gene72156:CDS | 45.0% | |
| ACCAGGAAATTCAGTGTCCA+TGG | + | chr4.1:6095690-6095709 | None:intergenic | 45.0% | |
| AGCGGTTAGGCAAATTCCTT+CGG | + | chr4.1:6096105-6096124 | None:intergenic | 45.0% | |
| AGTCGCTACAACACTTAACG+TGG | - | chr4.1:6096228-6096247 | MS.gene72156:CDS | 45.0% | |
| ATCACCCTCTCTGATGGTAA+CGG | - | chr4.1:6095812-6095831 | MS.gene72156:CDS | 45.0% | |
| CTACAACACTTAACGTGGGT+CGG | - | chr4.1:6096233-6096252 | MS.gene72156:CDS | 45.0% | |
| GAATTTGCCTAACCGCTTAG+AGG | - | chr4.1:6096108-6096127 | MS.gene72156:CDS | 45.0% | |
| GCGCAATTTATGTCATGGTG+TGG | - | chr4.1:6095964-6095983 | MS.gene72156:CDS | 45.0% | |
| GCTGAATTGATGCTCAGTTC+TGG | - | chr4.1:6096001-6096020 | MS.gene72156:CDS | 45.0% | |
| GTCGCTACAACACTTAACGT+GGG | - | chr4.1:6096229-6096248 | MS.gene72156:CDS | 45.0% | |
| GTTACCGTTACCATCAGAGA+GGG | + | chr4.1:6095819-6095838 | None:intergenic | 45.0% | |
| TACAACACTTAACGTGGGTC+GGG | - | chr4.1:6096234-6096253 | MS.gene72156:CDS | 45.0% | |
| TCAGTGTCCATGGAGATGAA+AGG | + | chr4.1:6095680-6095699 | None:intergenic | 45.0% | |
| TCCATGGACACTGAATTTCC+TGG | - | chr4.1:6095686-6095705 | MS.gene72156:CDS | 45.0% | |
| TTGGGTGCATAAATCACACC+AGG | + | chr4.1:6095707-6095726 | None:intergenic | 45.0% | |
| ! | GGTCTGAGGGTTTAAGATGA+CGG | + | chr4.1:6095739-6095758 | None:intergenic | 45.0% |
| ! | TTTTGCAACGCTTTGTACGG+TGG | - | chr4.1:6096196-6096215 | MS.gene72156:CDS | 45.0% |
| !!! | TGATTGGCTTTTCCTTCAGC+TGG | + | chr4.1:6095586-6095605 | None:intergenic | 45.0% |
| !!! | AATATTTTAACAATATTATT+TGG | - | chr4.1:6096139-6096158 | MS.gene72156:CDS | 5.0% |
| ATGAGGCTCAAAAACACGCC+GGG | - | chr4.1:6096344-6096363 | MS.gene72156:CDS | 50.0% | |
| ATTGACATGCTCTGTCGCCA+AGG | - | chr4.1:6095929-6095948 | MS.gene72156:CDS | 50.0% | |
| CAGAGAGGGTGATACCAACT+TGG | + | chr4.1:6095805-6095824 | None:intergenic | 50.0% | |
| GCGGTTAGGCAAATTCCTTC+GGG | + | chr4.1:6096104-6096123 | None:intergenic | 50.0% | |
| GCTGGAAAGTCTCATCAAGC+TGG | - | chr4.1:6096259-6096278 | MS.gene72156:CDS | 50.0% | |
| GGTTACCGTTACCATCAGAG+AGG | + | chr4.1:6095820-6095839 | None:intergenic | 50.0% | |
| GTTGGTATCACCCTCTCTGA+TGG | - | chr4.1:6095806-6095825 | MS.gene72156:CDS | 50.0% | |
| TGCGCTTGAAGTCAATCCCT+TGG | + | chr4.1:6095949-6095968 | None:intergenic | 50.0% | |
| TGGTGAAGATCTTGACCCGA+AGG | - | chr4.1:6096086-6096105 | MS.gene72156:CDS | 50.0% | |
| TTGACATGCTCTGTCGCCAA+GGG | - | chr4.1:6095930-6095949 | MS.gene72156:CDS | 50.0% | |
| ! | AAGATGACGGCGGTCAACTT+TGG | + | chr4.1:6095726-6095745 | None:intergenic | 50.0% |
| ! | AGATGACGGCGGTCAACTTT+GGG | + | chr4.1:6095725-6095744 | None:intergenic | 50.0% |
| ! | CTGAGGGTTTAAGATGACGG+CGG | + | chr4.1:6095736-6095755 | None:intergenic | 50.0% |
| !! | AAACACGCCGGGGTCTTATT+TGG | - | chr4.1:6096355-6096374 | MS.gene72156:CDS | 50.0% |
| TGAGGCTCAAAAACACGCCG+GGG | - | chr4.1:6096345-6096364 | MS.gene72156:CDS | 55.0% | |
| ! | CTTAACGTGGGTCGGGTTGC+TGG | - | chr4.1:6096241-6096260 | MS.gene72156:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 6095578 | 6096396 | 6095578 | ID=MS.gene72156 |
| chr4.1 | mRNA | 6095578 | 6096396 | 6095578 | ID=MS.gene72156.t1;Parent=MS.gene72156 |
| chr4.1 | exon | 6095578 | 6096396 | 6095578 | ID=MS.gene72156.t1.exon1;Parent=MS.gene72156.t1 |
| chr4.1 | CDS | 6095578 | 6096396 | 6095578 | ID=cds.MS.gene72156.t1;Parent=MS.gene72156.t1 |
| Gene Sequence |
| Protein sequence |
