AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72456

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72456.t1 MTR_2g073500 95.129 349 16 1 1 349 1 348 0.0 690
MS.gene72456.t1 MTR_1g111010 63.248 351 116 5 1 347 1 342 2.66e-163 460
MS.gene72456.t1 MTR_2g073510 51.988 327 139 5 28 343 31 350 6.05e-123 358
MS.gene72456.t1 MTR_3g049330 51.368 329 141 6 30 347 31 351 3.80e-120 350
MS.gene72456.t1 MTR_4g088030 46.667 360 170 8 1 347 1 351 1.10e-111 329
MS.gene72456.t1 MTR_2g015720 48.204 334 153 7 23 346 35 358 2.86e-111 328
MS.gene72456.t1 MTR_3g035830 47.337 338 153 8 22 346 23 348 9.21e-110 324
MS.gene72456.t1 MTR_5g034810 47.734 331 152 8 30 349 35 355 1.44e-109 324
MS.gene72456.t1 MTR_4g087730 49.085 328 148 7 30 347 45 363 9.76e-109 322
MS.gene72456.t1 MTR_4g079700 49.235 327 146 6 30 346 47 363 2.09e-108 321
MS.gene72456.t1 MTR_3g021060 46.176 340 158 8 22 348 38 365 4.03e-107 318
MS.gene72456.t1 MTR_3g021090 46.866 335 151 9 22 342 23 344 1.83e-104 313
MS.gene72456.t1 MTR_2g091175 46.505 329 154 8 30 347 50 367 2.09e-104 311
MS.gene72456.t1 MTR_5g071350 42.857 364 160 10 23 347 46 400 2.00e-94 287
MS.gene72456.t1 MTR_3g464570 41.329 346 170 8 27 347 40 377 2.76e-91 278
MS.gene72456.t1 MTR_1g112210 42.857 336 165 9 28 346 57 382 1.65e-88 271
MS.gene72456.t1 MTR_1g050448 42.090 335 164 8 30 346 53 375 2.07e-88 270
MS.gene72456.t1 MTR_1g112210 42.857 336 152 10 28 346 57 369 5.07e-87 267
MS.gene72456.t1 MTR_0136s0010 40.058 347 173 9 30 347 170 510 2.87e-82 259
MS.gene72456.t1 MTR_7g093450 40.058 347 173 10 30 347 248 588 3.47e-82 261
MS.gene72456.t1 MTR_4g121560 40.058 347 173 13 30 347 114 454 1.82e-79 250
MS.gene72456.t1 MTR_5g059400 38.983 354 176 10 26 347 171 516 3.42e-78 249
MS.gene72456.t1 MTR_1g116130 38.983 354 176 10 26 347 171 516 3.42e-78 249
MS.gene72456.t1 MTR_7g109550 39.181 342 172 12 30 346 137 467 3.68e-76 242
MS.gene72456.t1 MTR_4g126950 37.069 348 184 12 28 347 107 447 2.10e-75 239
MS.gene72456.t1 MTR_3g090120 38.398 362 191 11 10 348 109 461 1.05e-74 238
MS.gene72456.t1 MTR_5g033800 35.574 357 196 10 20 348 135 485 1.31e-74 239
MS.gene72456.t1 MTR_8g069790 35.511 352 193 11 25 348 173 518 2.12e-71 231
MS.gene72456.t1 MTR_4g016940 35.714 350 188 9 28 346 84 427 3.06e-71 228
MS.gene72456.t1 MTR_1g058380 36.782 348 187 11 28 347 53 395 1.27e-70 226
MS.gene72456.t1 MTR_3g464570 40.809 272 134 6 101 347 4 273 4.10e-68 215
MS.gene72456.t1 MTR_7g094750 36.415 357 176 12 30 348 91 434 7.82e-68 220
MS.gene72456.t1 MTR_3g070900 34.746 354 192 10 27 347 103 450 2.19e-67 219
MS.gene72456.t1 MTR_3g071650 36.467 351 182 10 30 347 89 431 3.90e-67 218
MS.gene72456.t1 MTR_8g463790 32.231 363 202 10 20 349 61 412 5.30e-65 211
MS.gene72456.t1 MTR_8g093530 36.127 346 189 10 25 349 85 419 7.88e-65 211
MS.gene72456.t1 MTR_4g014440 31.738 397 214 12 1 347 19 408 4.09e-64 209
MS.gene72456.t1 MTR_2g022560 37.714 350 180 11 30 348 97 439 1.68e-63 209
MS.gene72456.t1 MTR_4g014430 34.097 349 192 10 28 346 106 446 1.73e-62 206
MS.gene72456.t1 MTR_5g083260 35.674 356 184 13 27 347 80 425 1.96e-61 202
MS.gene72456.t1 MTR_7g068920 33.807 352 192 10 28 346 78 421 1.10e-60 201
MS.gene72456.t1 MTR_7g070060 32.203 354 197 9 30 347 64 410 1.42e-59 197
MS.gene72456.t1 MTR_5g082880 33.051 354 195 11 30 347 165 512 4.12e-58 196
MS.gene72456.t1 MTR_5g033800 34.400 250 148 6 20 259 135 378 2.06e-49 170
MS.gene72456.t1 MTR_7g094750 35.341 249 129 7 30 259 91 326 2.28e-44 155
MS.gene72456.t1 MTR_7g070060 31.081 296 166 7 88 347 15 308 1.02e-43 153
MS.gene72456.t1 MTR_7g070060 31.224 237 151 5 30 259 64 295 5.41e-39 140
MS.gene72456.t1 MTR_5g069320 34.686 271 133 9 82 339 147 386 1.24e-38 141
MS.gene72456.t1 MTR_6g061710 29.032 372 195 14 25 346 162 514 7.32e-34 131
MS.gene72456.t1 MTR_5g035150 29.609 358 206 14 27 346 82 431 2.05e-33 128
MS.gene72456.t1 MTR_8g093870 28.161 348 217 10 27 346 130 472 2.55e-32 126
MS.gene72456.t1 MTR_1g076690 28.809 361 202 14 25 342 218 566 1.01e-31 125
MS.gene72456.t1 MTR_4g092830 25.770 357 217 14 30 348 48 394 1.03e-31 124
MS.gene72456.t1 MTR_5g035090 27.195 353 211 12 27 342 80 423 1.96e-30 120
MS.gene72456.t1 MTR_2g016060 27.869 366 212 15 26 348 46 402 8.44e-29 115
MS.gene72456.t1 MTR_5g035080 27.049 366 204 15 27 346 72 420 2.50e-28 114
MS.gene72456.t1 MTR_4g125060 28.367 349 213 12 28 346 130 471 4.97e-28 114
MS.gene72456.t1 MTR_4g125060 28.367 349 213 12 28 346 198 539 7.86e-28 114
MS.gene72456.t1 MTR_4g125060 28.367 349 213 12 28 346 169 510 1.07e-27 113
MS.gene72456.t1 MTR_8g104970 27.978 361 199 16 27 342 117 461 3.40e-27 112
MS.gene72456.t1 MTR_6g016880 28.212 358 200 13 30 346 78 419 5.48e-27 110
MS.gene72456.t1 MTR_6g007777 25.562 356 218 13 27 346 113 457 9.92e-27 110
MS.gene72456.t1 MTR_3g109840 28.212 358 193 16 30 342 73 411 1.43e-26 109
MS.gene72456.t1 MTR_7g033230 27.147 361 212 11 26 346 260 609 1.44e-26 110
MS.gene72456.t1 MTR_7g033230 28.142 366 202 13 26 346 330 679 3.96e-26 109
MS.gene72456.t1 MTR_6g016240 26.124 356 214 14 30 346 71 416 4.86e-26 108
MS.gene72456.t1 MTR_5g035070 28.451 355 208 13 30 346 87 433 6.32e-26 108
MS.gene72456.t1 MTR_8g027900 26.536 358 215 12 28 347 209 556 1.78e-25 107
MS.gene72456.t1 MTR_7g019120 27.066 351 213 10 30 346 79 420 1.60e-24 103
MS.gene72456.t1 MTR_6g016875 25.568 352 220 11 30 346 64 408 4.06e-24 102
MS.gene72456.t1 MTR_3g021150 43.519 108 46 3 222 316 1 106 1.87e-23 94.0
MS.gene72456.t1 MTR_8g099300 24.586 362 226 10 24 347 68 420 1.04e-22 98.6
MS.gene72456.t1 MTR_5g035120 41.837 98 51 2 27 124 72 163 6.45e-19 83.6
MS.gene72456.t1 MTR_7g019140 40.000 95 51 1 30 124 79 167 1.32e-16 77.0
MS.gene72456.t1 MTR_2g022580 32.500 120 67 4 96 202 2 120 6.75e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72456.t1 AT1G78710 56.839 329 124 6 30 348 38 358 1.08e-136 393
MS.gene72456.t1 AT2G30900 50.904 332 143 7 30 348 43 367 1.22e-116 342
MS.gene72456.t1 AT1G29050 49.697 330 141 9 30 346 61 378 1.93e-112 332
MS.gene72456.t1 AT3G14850 49.552 335 145 8 28 348 32 356 4.83e-109 323
MS.gene72456.t1 AT2G42570 48.640 331 147 8 30 348 47 366 2.07e-108 321
MS.gene72456.t1 AT2G31110 47.720 329 149 8 30 346 45 362 2.67e-107 318
MS.gene72456.t1 AT2G34070 47.720 329 150 9 30 346 65 383 3.41e-105 314
MS.gene72456.t1 AT1G78710 49.689 322 105 8 30 348 31 298 9.07e-102 302
MS.gene72456.t1 AT5G58600 42.899 345 161 11 30 347 66 401 4.10e-86 266
MS.gene72456.t1 AT2G30900 55.310 226 93 4 30 254 43 261 3.72e-85 259
MS.gene72456.t1 AT3G54260 40.179 336 176 8 30 349 51 377 1.41e-84 261
MS.gene72456.t1 AT2G30010 40.336 357 171 10 24 347 50 397 3.74e-83 258
MS.gene72456.t1 AT2G30010 40.336 357 171 10 24 347 93 440 7.51e-83 258
MS.gene72456.t1 AT5G06700 39.481 347 175 10 30 347 255 595 3.28e-82 261
MS.gene72456.t1 AT3G14850 49.027 257 113 6 106 348 1 253 2.51e-80 246
MS.gene72456.t1 AT3G12060 38.395 349 180 9 28 347 194 536 4.89e-80 254
MS.gene72456.t1 AT3G62390 38.192 343 183 9 28 347 136 472 3.53e-79 250
MS.gene72456.t1 AT5G49340 40.456 351 168 12 30 347 100 442 2.52e-77 244
MS.gene72456.t1 AT2G31110 49.333 225 106 4 30 254 45 261 1.92e-76 236
MS.gene72456.t1 AT2G34070 47.036 253 118 7 106 346 1 249 3.92e-76 235
MS.gene72456.t1 AT1G60790 37.322 351 186 9 28 348 188 534 1.21e-73 238
MS.gene72456.t1 AT5G20590 36.812 345 186 11 30 347 141 480 7.09e-73 234
MS.gene72456.t1 AT1G48880 38.938 339 175 11 30 348 115 441 1.26e-72 232
MS.gene72456.t1 AT3G11030 37.500 352 178 12 30 347 106 449 1.26e-65 214
MS.gene72456.t1 AT3G06080 36.648 352 183 12 30 348 103 447 1.27e-65 214
MS.gene72456.t1 AT5G06230 35.613 351 197 8 25 347 16 365 1.03e-64 209
MS.gene72456.t1 AT5G01620 32.571 350 199 9 27 346 107 449 1.39e-64 211
MS.gene72456.t1 AT5G01620 32.571 350 199 9 27 346 107 449 1.39e-64 211
MS.gene72456.t1 AT5G01360 32.479 351 199 9 28 346 34 378 1.51e-64 209
MS.gene72456.t1 AT5G01620 32.571 350 199 9 27 346 115 457 2.06e-64 211
MS.gene72456.t1 AT2G40160 34.366 355 189 10 30 347 77 424 2.25e-64 211
MS.gene72456.t1 AT5G06230 35.613 351 197 8 25 347 57 406 2.41e-64 210
MS.gene72456.t1 AT2G40160 34.000 350 187 9 30 342 77 419 5.73e-64 209
MS.gene72456.t1 AT5G01360 32.386 352 198 10 28 346 90 434 6.06e-64 209
MS.gene72456.t1 AT3G55990 33.428 353 196 10 28 347 138 484 2.98e-63 209
MS.gene72456.t1 AT5G19160 35.311 354 188 11 30 348 99 446 3.08e-63 208
MS.gene72456.t1 AT2G40320 35.028 354 189 10 27 347 78 423 5.34e-63 207
MS.gene72456.t1 AT2G31110 47.596 208 97 5 149 346 9 214 1.69e-62 199
MS.gene72456.t1 AT3G11570 36.875 320 175 9 55 347 101 420 7.07e-62 204
MS.gene72456.t1 AT1G73140 33.056 360 195 11 23 348 60 407 4.64e-61 201
MS.gene72456.t1 AT5G58600 43.777 233 119 7 30 259 66 289 2.42e-59 193
MS.gene72456.t1 AT2G40150 32.213 357 197 9 30 347 71 421 5.40e-59 196
MS.gene72456.t1 AT3G62390 37.349 249 140 5 28 266 136 378 1.51e-56 189
MS.gene72456.t1 AT2G38320 29.859 355 210 9 28 348 57 406 1.15e-55 187
MS.gene72456.t1 AT5G20590 35.270 241 140 6 30 259 141 376 9.73e-50 171
MS.gene72456.t1 AT2G38320 29.653 317 189 7 66 348 7 323 7.67e-49 167
MS.gene72456.t1 AT2G40160 36.929 241 136 5 30 260 77 311 5.91e-48 164
MS.gene72456.t1 AT3G06080 38.272 243 130 7 30 259 103 338 5.42e-47 162
MS.gene72456.t1 AT5G19160 35.537 242 138 5 30 259 99 334 1.35e-44 156
MS.gene72456.t1 AT5G06230 34.274 248 149 4 25 259 57 303 7.32e-43 151
MS.gene72456.t1 AT5G64470 28.612 353 211 12 29 349 54 397 2.28e-38 141
MS.gene72456.t1 AT5G64470 28.134 359 211 13 29 349 54 403 3.91e-38 140
MS.gene72456.t1 AT1G70230 30.000 350 195 14 30 346 79 411 1.31e-37 139
MS.gene72456.t1 AT3G28150 27.500 360 219 12 22 348 62 412 3.08e-33 127
MS.gene72456.t1 AT5G51640 28.841 371 210 12 23 349 131 491 4.07e-33 129
MS.gene72456.t1 AT4G25360 28.142 366 214 10 25 349 170 527 1.96e-31 124
MS.gene72456.t1 AT4G25360 28.142 366 214 10 25 349 170 527 1.96e-31 124
MS.gene72456.t1 AT5G64020 28.375 363 203 15 30 346 57 408 7.83e-29 115
MS.gene72456.t1 AT2G14530 26.503 366 207 13 26 346 58 406 8.58e-29 115
MS.gene72456.t1 AT1G01430 26.823 384 227 13 7 347 76 448 1.66e-28 115
MS.gene72456.t1 AT5G20680 26.611 357 211 14 27 346 115 457 4.85e-28 114
MS.gene72456.t1 AT5G20680 26.761 355 213 12 27 346 191 533 5.51e-28 114
MS.gene72456.t1 AT5G20680 26.761 355 213 12 27 346 209 551 6.08e-28 114
MS.gene72456.t1 AT5G20680 26.761 355 213 12 27 346 209 551 6.08e-28 114
MS.gene72456.t1 AT5G20680 26.761 355 213 12 27 346 209 551 6.08e-28 114
MS.gene72456.t1 AT5G20680 26.761 355 213 12 27 346 209 551 6.08e-28 114
MS.gene72456.t1 AT2G37720 26.705 352 211 13 30 346 143 482 3.06e-26 109
MS.gene72456.t1 AT5G15900 28.689 366 189 13 30 346 67 409 4.07e-26 108
MS.gene72456.t1 AT5G15900 28.689 366 189 13 30 346 86 428 4.74e-26 108
MS.gene72456.t1 AT4G01080 26.546 388 230 12 1 347 58 431 1.10e-25 107
MS.gene72456.t1 AT5G15890 27.123 365 201 13 27 349 184 525 2.47e-24 104
MS.gene72456.t1 AT3G02440 28.531 354 204 15 30 346 135 476 2.31e-23 101
MS.gene72456.t1 AT3G02440 28.531 354 204 15 30 346 127 468 2.39e-23 100
MS.gene72456.t1 AT5G64470 27.490 251 156 9 29 262 54 295 7.99e-23 97.4
MS.gene72456.t1 AT5G64470 27.490 251 156 9 29 262 54 295 1.03e-22 97.4
MS.gene72456.t1 AT4G23790 25.967 362 216 14 28 347 77 428 3.70e-20 91.3
MS.gene72456.t1 AT4G11090 23.677 359 226 14 28 347 77 426 3.91e-17 82.4

Find 72 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAAGGTATAAGTTGAATT+TGG 0.153086 2.3:-22546536 None:intergenic
TTGCCACATGTTATTGCTTA+TGG 0.235427 2.3:-22546483 None:intergenic
AGTTGGGAATGAATTAATTA+AGG 0.268562 2.3:+22546984 MS.gene72456:CDS
AACATTTCATTCCAAGTATC+AGG 0.294167 2.3:-22547925 None:intergenic
AAATGGGTTCTTAGTAGATT+TGG 0.303016 2.3:+22546729 MS.gene72456:CDS
GAAGAGCATCATAAGTAAAA+TGG 0.310498 2.3:+22547777 MS.gene72456:CDS
TTAAGGTATAAGTTGAATTT+GGG 0.319731 2.3:-22546535 None:intergenic
TTGCTGGTGTTCCTGATACT+TGG 0.354133 2.3:+22547914 MS.gene72456:CDS
TACTAGCTTCAGCATTTCTA+TGG 0.355586 2.3:+22545268 MS.gene72456:CDS
CATGAAAATTCTAGTGGAAA+AGG 0.364151 2.3:+22547691 MS.gene72456:intron
TGTAGTCACTGGTGTCTTGC+TGG 0.365563 2.3:+22547898 MS.gene72456:CDS
AATTAAGGAGATGGATCATT+TGG 0.368405 2.3:+22546999 MS.gene72456:CDS
TCAAAGGACTGTCCTTTCAT+TGG 0.370728 2.3:+22545393 MS.gene72456:CDS
GGTGGCATTGGTCCTTGATC+TGG 0.377983 2.3:-22547733 None:intergenic
TATCATTGGTGGACTCATAC+TGG 0.389613 2.3:+22546844 MS.gene72456:CDS
AAGATTATATCAAGTATAGA+TGG 0.389720 2.3:+22545454 MS.gene72456:CDS
AAACAAAACTCTAGTCTTTG+AGG 0.395591 2.3:-22547079 None:intergenic
TTTGGTTCATGATAAAGAAA+AGG 0.411976 2.3:+22546747 MS.gene72456:CDS
AATGTTGGAATCAACCCATT+TGG 0.413535 2.3:-22547052 None:intergenic
TTGGTTCATGATAAAGAAAA+GGG 0.414359 2.3:+22546748 MS.gene72456:CDS
GATTAAGCACTTGGGCCAAA+TGG 0.421751 2.3:+22547037 MS.gene72456:CDS
ACTCACTGTGTTCTCAATTC+CGG 0.446901 2.3:+22546574 MS.gene72456:CDS
TCACTTTGCTAACACAGTTA+AGG 0.449047 2.3:+22547824 MS.gene72456:CDS
AACAAAACTCTAGTCTTTGA+GGG 0.451994 2.3:-22547078 None:intergenic
AGGAAAGAAGATAATGTTTG+TGG 0.452207 2.3:+22546454 MS.gene72456:CDS
ATTAAGCACTTGGGCCAAAT+GGG 0.476158 2.3:+22547038 MS.gene72456:CDS
GTGATTACTCTCAAGGCAAC+TGG 0.476382 2.3:+22545322 MS.gene72456:CDS
ATGAAAATTCTAGTGGAAAA+GGG 0.480566 2.3:+22547692 MS.gene72456:intron
GACTGTCCTTTCATTGGTCA+AGG 0.484445 2.3:+22545399 MS.gene72456:CDS
CTAGTAGCAGAGAACATAGT+TGG 0.496126 2.3:-22545251 None:intergenic
TTAAGATTGGATTAAGCACT+TGG 0.499090 2.3:+22547028 MS.gene72456:CDS
TTGTTAACAGGTTTGATGGT+GGG 0.501332 2.3:+22546411 MS.gene72456:intron
AGGTTGGACACAATTAGCTC+AGG 0.509177 2.3:+22546781 MS.gene72456:CDS
AGTCTTTGAGGGTTCAATGT+TGG 0.510727 2.3:-22547067 None:intergenic
GAATGAATTAATTAAGGAGA+TGG 0.511939 2.3:+22546990 MS.gene72456:CDS
CCGTCTATCTACACCGGCCG+TGG 0.512023 2.3:+22547859 MS.gene72456:CDS
CCACGGCCGGTGTAGATAGA+CGG 0.514592 2.3:-22547859 None:intergenic
GGAAAGAAGATAATGTTTGT+GGG 0.515523 2.3:+22546455 MS.gene72456:CDS
GGACCAATGCCACCTTATCC+TGG 0.516333 2.3:+22547742 MS.gene72456:CDS
CCCTCTCTATGATGCTTCAA+AGG 0.517294 2.3:+22545377 MS.gene72456:CDS
CCTTCAGTTGTAATCTTCCA+AGG 0.529995 2.3:+22545481 MS.gene72456:CDS
CAATTAGCTCAGGAAATCAA+TGG 0.532345 2.3:+22546791 MS.gene72456:CDS
TCACATGGTTGTTGTAGAGA+AGG 0.535627 2.3:-22545303 None:intergenic
AGCTCAGGAAATCAATGGAA+AGG 0.538311 2.3:+22546796 MS.gene72456:CDS
CCTTGGAAGATTACAACTGA+AGG 0.538678 2.3:-22545481 None:intergenic
ACACCAGGATAAGGTGGCAT+TGG 0.545022 2.3:-22547745 None:intergenic
GACATAACTTTCGCCACGGC+CGG 0.547140 2.3:-22547872 None:intergenic
ACAGTGTCTACACCAGGATA+AGG 0.551553 2.3:-22547754 None:intergenic
CACGGCCGGTGTAGATAGAC+GGG 0.552288 2.3:-22547858 None:intergenic
AGACAAACTAAACCAGATCA+AGG 0.552461 2.3:+22547721 MS.gene72456:CDS
CTTGGAAGATTACAACTGAA+GGG 0.558850 2.3:-22545480 None:intergenic
CTCTTCACAGTGTCTACACC+AGG 0.559569 2.3:-22547760 None:intergenic
TTTGTTAACAGGTTTGATGG+TGG 0.560255 2.3:+22546410 MS.gene72456:intron
CTTTGCTAACACAGTTAAGG+AGG 0.560315 2.3:+22547827 MS.gene72456:CDS
GTGATGTCAAGCAATTGTAC+TGG 0.560712 2.3:-22547805 None:intergenic
CAACCATGTGATTACTCTCA+AGG 0.576637 2.3:+22545315 MS.gene72456:CDS
ATCATAGAGAGGGTAGGAGA+AGG 0.576762 2.3:-22545367 None:intergenic
GTTATGTCGACTGTAGTCAC+TGG 0.597307 2.3:+22547887 MS.gene72456:CDS
GTAGCAGAGAACATAGTTGG+TGG 0.598237 2.3:-22545248 None:intergenic
CTAACACAGTTAAGGAGGGA+TGG 0.604483 2.3:+22547832 MS.gene72456:CDS
TCAAAACCTTGACCAATGAA+AGG 0.604578 2.3:-22545405 None:intergenic
TCCTTTGAAGCATCATAGAG+AGG 0.605006 2.3:-22545378 None:intergenic
TAAGATTGGATTAAGCACTT+GGG 0.608859 2.3:+22547029 MS.gene72456:CDS
CCTTTGAAGCATCATAGAGA+GGG 0.612469 2.3:-22545377 None:intergenic
TGAAGCATCATAGAGAGGGT+AGG 0.612829 2.3:-22545373 None:intergenic
AATATGAAGCTTCAATCATG+TGG 0.641893 2.3:+22546701 MS.gene72456:CDS
TTTGCTAACACAGTTAAGGA+GGG 0.649121 2.3:+22547828 MS.gene72456:CDS
ATTCCATAAGCAATAACATG+TGG 0.651581 2.3:+22546480 MS.gene72456:CDS
GTGTCTACACCAGGATAAGG+TGG 0.667667 2.3:-22547751 None:intergenic
TTGCCTTGAGAGTAATCACA+TGG 0.671880 2.3:-22545318 None:intergenic
AGTCGACATAACTTTCGCCA+CGG 0.676539 2.3:-22547876 None:intergenic
GGTCACCCGTCTATCTACAC+CGG 0.714846 2.3:+22547853 MS.gene72456:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATCAAGATTTTAACAAA+AGG - chr2.3:22547536-22547555 None:intergenic 15.0%
!!! TGAATTTTATTATGATGAAA+TGG + chr2.3:22547599-22547618 MS.gene72456:intron 15.0%
!! AAGATTATATCAAGTATAGA+TGG + chr2.3:22545454-22545473 MS.gene72456:CDS 20.0%
!! ATTCAATGTTTATGTTTGAT+CGG + chr2.3:22545941-22545960 MS.gene72456:intron 20.0%
!! GAAAATAAAAACAAGAACTT+TGG - chr2.3:22545564-22545583 None:intergenic 20.0%
!! GCATGAATTATATATAAGTA+AGG + chr2.3:22547224-22547243 MS.gene72456:intron 20.0%
!!! AATTTTGGTATTTTTATGTG+AGG + chr2.3:22546900-22546919 MS.gene72456:intron 20.0%
!!! ATTTAATTGTTTTCACAGAT+GGG + chr2.3:22546951-22546970 MS.gene72456:intron 20.0%
!!! ATTTCTTTGAACTCAAATTT+TGG + chr2.3:22546885-22546904 MS.gene72456:intron 20.0%
!!! TATTTAATTGTTTTCACAGA+TGG + chr2.3:22546950-22546969 MS.gene72456:intron 20.0%
!!! TGAGTTAAAGTTTTGTTTTT+TGG + chr2.3:22545514-22545533 MS.gene72456:intron 20.0%
!!! TGATTTTCAATACATATCAT+TGG + chr2.3:22546830-22546849 MS.gene72456:CDS 20.0%
!!! TGATTTTTGTTTGAAACTAT+AGG + chr2.3:22545617-22545636 MS.gene72456:intron 20.0%
!!! TTAAATGATTTTGTCTAATG+TGG + chr2.3:22546657-22546676 MS.gene72456:intron 20.0%
!!! TTAAGGTATAAGTTGAATTT+GGG - chr2.3:22546538-22546557 None:intergenic 20.0%
!!! TTATTTTTTAGCTTCTCTAA+TGG + chr2.3:22546385-22546404 MS.gene72456:intron 20.0%
!!! TTCTTTTGTTTGTTTTGTTA+AGG - chr2.3:22546555-22546574 None:intergenic 20.0%
! AGTTGGGAATGAATTAATTA+AGG + chr2.3:22546984-22547003 MS.gene72456:CDS 25.0%
! ATGAAAATTCTAGTGGAAAA+GGG + chr2.3:22547692-22547711 MS.gene72456:intron 25.0%
! CTCAAAAACATAGTTGAAAA+TGG - chr2.3:22545870-22545889 None:intergenic 25.0%
! GAATGAATTAATTAAGGAGA+TGG + chr2.3:22546990-22547009 MS.gene72456:CDS 25.0%
! TAACTCAAATATAACTACCT+TGG - chr2.3:22545501-22545520 None:intergenic 25.0%
! TAGTAATTCAATGCTTATGT+TGG + chr2.3:22546076-22546095 MS.gene72456:intron 25.0%
! TATAAGCTAAAGAATAGTAC+CGG - chr2.3:22546596-22546615 None:intergenic 25.0%
! TTGGTTCATGATAAAGAAAA+GGG + chr2.3:22546748-22546767 MS.gene72456:CDS 25.0%
!! GTTAAGGTATAAGTTGAATT+TGG - chr2.3:22546539-22546558 None:intergenic 25.0%
!! TTTGGAATGTATCTTCAAAT+GGG + chr2.3:22545532-22545551 MS.gene72456:intron 25.0%
!! TTTGGTTCATGATAAAGAAA+AGG + chr2.3:22546747-22546766 MS.gene72456:CDS 25.0%
!! TTTTCAATACATATCATTGG+TGG + chr2.3:22546833-22546852 MS.gene72456:CDS 25.0%
!! TTTTGTCTAATGTGGTTTTT+TGG + chr2.3:22546665-22546684 MS.gene72456:intron 25.0%
!!! AAGTTTTTGGAAAGATACAA+AGG + chr2.3:22546434-22546453 MS.gene72456:CDS 25.0%
!!! ACATAGTTGACTATTTTTAG+TGG + chr2.3:22545839-22545858 MS.gene72456:intron 25.0%
!!! CATAGTTGACTATTTTTAGT+GGG + chr2.3:22545840-22545859 MS.gene72456:intron 25.0%
!!! TTTGTCTAATGTGGTTTTTT+GGG + chr2.3:22546666-22546685 MS.gene72456:intron 25.0%
!!! TTTGTTGTTGTTATTGTTGT+TGG + chr2.3:22545756-22545775 MS.gene72456:intron 25.0%
!!! TTTTGGAATGTATCTTCAAA+TGG + chr2.3:22545531-22545550 MS.gene72456:intron 25.0%
AAATCCAAACATTCATTGAG+TGG - chr2.3:22547150-22547169 None:intergenic 30.0%
AACATTTCATTCCAAGTATC+AGG - chr2.3:22547928-22547947 None:intergenic 30.0%
AATATGAAGCTTCAATCATG+TGG + chr2.3:22546701-22546720 MS.gene72456:CDS 30.0%
AATTAAGGAGATGGATCATT+TGG + chr2.3:22546999-22547018 MS.gene72456:CDS 30.0%
AGACTTAGATGAATGTAATG+TGG + chr2.3:22546324-22546343 MS.gene72456:intron 30.0%
AGGAAAGAAGATAATGTTTG+TGG + chr2.3:22546454-22546473 MS.gene72456:CDS 30.0%
ATAGTCAATCATCCACAAAT+CGG - chr2.3:22545808-22545827 None:intergenic 30.0%
ATTCCATAAGCAATAACATG+TGG + chr2.3:22546480-22546499 MS.gene72456:CDS 30.0%
ATTCTCCTTAGCATCAATTT+CGG + chr2.3:22546147-22546166 MS.gene72456:intron 30.0%
CAATCATGTGGTTGAAAAAT+GGG + chr2.3:22546713-22546732 MS.gene72456:CDS 30.0%
CATGAAAATTCTAGTGGAAA+AGG + chr2.3:22547691-22547710 MS.gene72456:intron 30.0%
CATTCAGGAAAAGAAGAAAA+GGG - chr2.3:22547670-22547689 None:intergenic 30.0%
GAATGACATGAAAATTCTAG+TGG + chr2.3:22547685-22547704 MS.gene72456:intron 30.0%
GGAAAGAAGATAATGTTTGT+GGG + chr2.3:22546455-22546474 MS.gene72456:CDS 30.0%
TAAGATTGGATTAAGCACTT+GGG + chr2.3:22547029-22547048 MS.gene72456:CDS 30.0%
TCAATCATGTGGTTGAAAAA+TGG + chr2.3:22546712-22546731 MS.gene72456:CDS 30.0%
TCATTCAGGAAAAGAAGAAA+AGG - chr2.3:22547671-22547690 None:intergenic 30.0%
TTAAGATTGGATTAAGCACT+TGG + chr2.3:22547028-22547047 MS.gene72456:CDS 30.0%
TTTAAACTCGACACATTGTA+AGG + chr2.3:22546018-22546037 MS.gene72456:intron 30.0%
! AAACAAAACTCTAGTCTTTG+AGG - chr2.3:22547082-22547101 None:intergenic 30.0%
! AAATGGGTTCTTAGTAGATT+TGG + chr2.3:22546729-22546748 MS.gene72456:CDS 30.0%
! AACAAAACTCTAGTCTTTGA+GGG - chr2.3:22547081-22547100 None:intergenic 30.0%
! AAGAAAAGGGAAGAATTTTG+AGG + chr2.3:22546761-22546780 MS.gene72456:CDS 30.0%
! CTAGAATTTTCATGTCATTC+AGG - chr2.3:22547685-22547704 None:intergenic 30.0%
! CTCTAATGGTTTTTGTTAAC+AGG + chr2.3:22546399-22546418 MS.gene72456:intron 30.0%
! GTTTTTGTTAACAGGTTTGA+TGG + chr2.3:22546407-22546426 MS.gene72456:intron 30.0%
! TTGTTGTTGGTGTATGATAT+TGG + chr2.3:22545769-22545788 MS.gene72456:intron 30.0%
!! AAGTTTTGTCTCACTAAATG+AGG - chr2.3:22545738-22545757 None:intergenic 30.0%
!! GAAGAGCATCATAAGTAAAA+TGG + chr2.3:22547777-22547796 MS.gene72456:CDS 30.0%
!!! AAGACTAGAGTTTTGTTTCA+AGG + chr2.3:22547084-22547103 MS.gene72456:CDS 30.0%
!!! AGATGGGATTATTTTCAAGT+TGG + chr2.3:22546967-22546986 MS.gene72456:intron 30.0%
!!! CATTTGGAAGCTTTTAAGAT+TGG + chr2.3:22547015-22547034 MS.gene72456:CDS 30.0%
!!! GATGGGATTATTTTCAAGTT+GGG + chr2.3:22546968-22546987 MS.gene72456:intron 30.0%
!!! TTCAACTATGTTTTTGAGAC+CGG + chr2.3:22545872-22545891 MS.gene72456:intron 30.0%
!!! TTGTCTAATGTGGTTTTTTG+GGG + chr2.3:22546667-22546686 MS.gene72456:intron 30.0%
AATGTTGGAATCAACCCATT+TGG - chr2.3:22547055-22547074 None:intergenic 35.0%
AGACAAACTAAACCAGATCA+AGG + chr2.3:22547721-22547740 MS.gene72456:CDS 35.0%
CAATTAGCTCAGGAAATCAA+TGG + chr2.3:22546791-22546810 MS.gene72456:CDS 35.0%
CTTGGAAGATTACAACTGAA+GGG - chr2.3:22545483-22545502 None:intergenic 35.0%
GAATTTGAGCATGTGGTTAA+TGG + chr2.3:22546922-22546941 MS.gene72456:intron 35.0%
TATTTGTCATAGCTTCGTGT+CGG + chr2.3:22547407-22547426 MS.gene72456:intron 35.0%
TCAAAACCTTGACCAATGAA+AGG - chr2.3:22545408-22545427 None:intergenic 35.0%
TCACTTTGCTAACACAGTTA+AGG + chr2.3:22547824-22547843 MS.gene72456:CDS 35.0%
TCTTCCACTCAATGAATGTT+TGG + chr2.3:22547143-22547162 MS.gene72456:intron 35.0%
TTGCCACATGTTATTGCTTA+TGG - chr2.3:22546486-22546505 None:intergenic 35.0%
TTTGCTAACACAGTTAAGGA+GGG + chr2.3:22547828-22547847 MS.gene72456:CDS 35.0%
TTTGTTAACAGGTTTGATGG+TGG + chr2.3:22546410-22546429 MS.gene72456:intron 35.0%
! CCTTGTGATGGAAAAAAATG+TGG - chr2.3:22545980-22545999 None:intergenic 35.0%
! TACTAGCTTCAGCATTTCTA+TGG + chr2.3:22545268-22545287 MS.gene72456:CDS 35.0%
! TTGTTAACAGGTTTGATGGT+GGG + chr2.3:22546411-22546430 MS.gene72456:intron 35.0%
!! AGTAAGGTTTTATGCAGTCA+TGG + chr2.3:22547240-22547259 MS.gene72456:intron 35.0%
!! ATGAAGTTTCAACCGATTTG+TGG + chr2.3:22545793-22545812 MS.gene72456:intron 35.0%
!!! AAAGGGAAGAATTTTGAGGT+TGG + chr2.3:22546765-22546784 MS.gene72456:CDS 35.0%
!!! CCACATTTTTTTCCATCACA+AGG + chr2.3:22545977-22545996 MS.gene72456:intron 35.0%
!!! GGTTTTGATTGTCTTGGAAA+TGG + chr2.3:22545420-22545439 MS.gene72456:CDS 35.0%
!!! TCAACTATGTTTTTGAGACC+GGG + chr2.3:22545873-22545892 MS.gene72456:intron 35.0%
!!! TGTCTAATGTGGTTTTTTGG+GGG + chr2.3:22546668-22546687 MS.gene72456:intron 35.0%
ACAGATTGCAACGTATTCCA+CGG + chr2.3:22547490-22547509 MS.gene72456:intron 40.0%
ACTCACTGTGTTCTCAATTC+CGG + chr2.3:22546574-22546593 MS.gene72456:CDS 40.0%
AGCTCAGGAAATCAATGGAA+AGG + chr2.3:22546796-22546815 MS.gene72456:CDS 40.0%
ATGTGAGGAATTTGAGCATG+TGG + chr2.3:22546915-22546934 MS.gene72456:intron 40.0%
ATTAAGCACTTGGGCCAAAT+GGG + chr2.3:22547038-22547057 MS.gene72456:CDS 40.0%
CAACCATGTGATTACTCTCA+AGG + chr2.3:22545315-22545334 MS.gene72456:CDS 40.0%
CCTTCAGTTGTAATCTTCCA+AGG + chr2.3:22545481-22545500 MS.gene72456:CDS 40.0%
CCTTGGAAGATTACAACTGA+AGG - chr2.3:22545484-22545503 None:intergenic 40.0%
CTAGTAGCAGAGAACATAGT+TGG - chr2.3:22545254-22545273 None:intergenic 40.0%
CTCGAGTTTGATTCTTGTGA+TGG - chr2.3:22546276-22546295 None:intergenic 40.0%
CTTTGCTAACACAGTTAAGG+AGG + chr2.3:22547827-22547846 MS.gene72456:CDS 40.0%
GATCTGAGTCTGATTACACT+TGG + chr2.3:22545895-22545914 MS.gene72456:intron 40.0%
GTCAAATGAAGTCTCACTTG+TGG + chr2.3:22547304-22547323 MS.gene72456:intron 40.0%
GTCTGACACTTAATAGACAC+CGG + chr2.3:22547431-22547450 MS.gene72456:intron 40.0%
GTGATGTCAAGCAATTGTAC+TGG - chr2.3:22547808-22547827 None:intergenic 40.0%
GTTGCAATCTGTGTAACACT+GGG - chr2.3:22547482-22547501 None:intergenic 40.0%
TCAAAGGACTGTCCTTTCAT+TGG + chr2.3:22545393-22545412 MS.gene72456:CDS 40.0%
TCACATGGTTGTTGTAGAGA+AGG - chr2.3:22545306-22545325 None:intergenic 40.0%
TCCTTAGCATCAATTTCGGT+GGG + chr2.3:22546151-22546170 MS.gene72456:intron 40.0%
TTGCCTTGAGAGTAATCACA+TGG - chr2.3:22545321-22545340 None:intergenic 40.0%
! AGTCTTTGAGGGTTCAATGT+TGG - chr2.3:22547070-22547089 None:intergenic 40.0%
! GTTTTATGCAGTCATGGATG+TGG + chr2.3:22547246-22547265 MS.gene72456:intron 40.0%
! TTCTTGTGATGGAAACAACG+TGG - chr2.3:22546265-22546284 None:intergenic 40.0%
!! CCTTTGAAGCATCATAGAGA+GGG - chr2.3:22545380-22545399 None:intergenic 40.0%
!! TATCATTGGTGGACTCATAC+TGG + chr2.3:22546844-22546863 MS.gene72456:CDS 40.0%
!! TCCTTTGAAGCATCATAGAG+AGG - chr2.3:22545381-22545400 None:intergenic 40.0%
!!! GGTCAAGGTTTTGATTGTCT+TGG + chr2.3:22545414-22545433 MS.gene72456:CDS 40.0%
!!! GTCTAATGTGGTTTTTTGGG+GGG + chr2.3:22546669-22546688 MS.gene72456:intron 40.0%
!!! GTTTGATGGTGGGAAGTTTT+TGG + chr2.3:22546421-22546440 MS.gene72456:CDS 40.0%
ACACTTAATAGACACCGGAC+CGG + chr2.3:22547436-22547455 MS.gene72456:intron 45.0%
ACAGTGTCTACACCAGGATA+AGG - chr2.3:22547757-22547776 None:intergenic 45.0%
AGGTTGGACACAATTAGCTC+AGG + chr2.3:22546781-22546800 MS.gene72456:CDS 45.0%
AGTCGACATAACTTTCGCCA+CGG - chr2.3:22547879-22547898 None:intergenic 45.0%
ATCATAGAGAGGGTAGGAGA+AGG - chr2.3:22545370-22545389 None:intergenic 45.0%
CCCTCTCTATGATGCTTCAA+AGG + chr2.3:22545377-22545396 MS.gene72456:CDS 45.0%
CGTTGCAATCTGTGTAACAC+TGG - chr2.3:22547483-22547502 None:intergenic 45.0%
CTAACACAGTTAAGGAGGGA+TGG + chr2.3:22547832-22547851 MS.gene72456:CDS 45.0%
CTCCTTAGCATCAATTTCGG+TGG + chr2.3:22546150-22546169 MS.gene72456:intron 45.0%
GACTGTCCTTTCATTGGTCA+AGG + chr2.3:22545399-22545418 MS.gene72456:CDS 45.0%
GATTAAGCACTTGGGCCAAA+TGG + chr2.3:22547037-22547056 MS.gene72456:CDS 45.0%
GTAGCAGAGAACATAGTTGG+TGG - chr2.3:22545251-22545270 None:intergenic 45.0%
GTGATTACTCTCAAGGCAAC+TGG + chr2.3:22545322-22545341 MS.gene72456:CDS 45.0%
GTGTAATCAGACTCAGATCC+CGG - chr2.3:22545894-22545913 None:intergenic 45.0%
TTGCTGGTGTTCCTGATACT+TGG + chr2.3:22547914-22547933 MS.gene72456:CDS 45.0%
! CTTCAGATCAAAAGCGTGTC+CGG - chr2.3:22547458-22547477 None:intergenic 45.0%
! GTTATGTCGACTGTAGTCAC+TGG + chr2.3:22547887-22547906 MS.gene72456:CDS 45.0%
! TGAAGCATCATAGAGAGGGT+AGG - chr2.3:22545376-22545395 None:intergenic 45.0%
! TGTGCTACTCTCAACTCTCA+AGG + chr2.3:22546114-22546133 MS.gene72456:intron 45.0%
ACACCAGGATAAGGTGGCAT+TGG - chr2.3:22547748-22547767 None:intergenic 50.0%
CACAACTAGAATCTGAGCCG+TGG - chr2.3:22547510-22547529 None:intergenic 50.0%
CTCTTCACAGTGTCTACACC+AGG - chr2.3:22547763-22547782 None:intergenic 50.0%
GAGAGTTGAGAGTAGCACAC+TGG - chr2.3:22546113-22546132 None:intergenic 50.0%
GCCCACCGAAATTGATGCTA+AGG - chr2.3:22546155-22546174 None:intergenic 50.0%
GTGTCTACACCAGGATAAGG+TGG - chr2.3:22547754-22547773 None:intergenic 50.0%
GTTGAGAGTAGCACACTGGA+AGG - chr2.3:22546109-22546128 None:intergenic 50.0%
TGTAGTCACTGGTGTCTTGC+TGG + chr2.3:22547898-22547917 MS.gene72456:CDS 50.0%
! CTCGAGTTTGAGCCTTGTGA+TGG - chr2.3:22545992-22546011 None:intergenic 50.0%
!!! GGTTTTTTGGGGGGTGTCAT+AGG + chr2.3:22546678-22546697 MS.gene72456:intron 50.0%
GACATAACTTTCGCCACGGC+CGG - chr2.3:22547875-22547894 None:intergenic 55.0%
GGACCAATGCCACCTTATCC+TGG + chr2.3:22547742-22547761 MS.gene72456:CDS 55.0%
GGTCACCCGTCTATCTACAC+CGG + chr2.3:22547853-22547872 MS.gene72456:CDS 55.0%
! GATCAAAAGCGTGTCCGGTC+CGG - chr2.3:22547453-22547472 None:intergenic 55.0%
!! GGTGGCATTGGTCCTTGATC+TGG - chr2.3:22547736-22547755 None:intergenic 55.0%
CACGGCCGGTGTAGATAGAC+GGG - chr2.3:22547861-22547880 None:intergenic 60.0%
CCACGGCCGGTGTAGATAGA+CGG - chr2.3:22547862-22547881 None:intergenic 60.0%
CCGTCTATCTACACCGGCCG+TGG + chr2.3:22547859-22547878 MS.gene72456:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 22545234 22547972 22545234 ID=MS.gene72456
chr2.3 mRNA 22545234 22547972 22545234 ID=MS.gene72456.t1;Parent=MS.gene72456
chr2.3 exon 22545234 22545502 22545234 ID=MS.gene72456.t1.exon1;Parent=MS.gene72456.t1
chr2.3 CDS 22545234 22545502 22545234 ID=cds.MS.gene72456.t1;Parent=MS.gene72456.t1
chr2.3 exon 22546421 22546595 22546421 ID=MS.gene72456.t1.exon2;Parent=MS.gene72456.t1
chr2.3 CDS 22546421 22546595 22546421 ID=cds.MS.gene72456.t1;Parent=MS.gene72456.t1
chr2.3 exon 22546700 22546878 22546700 ID=MS.gene72456.t1.exon3;Parent=MS.gene72456.t1
chr2.3 CDS 22546700 22546878 22546700 ID=cds.MS.gene72456.t1;Parent=MS.gene72456.t1
chr2.3 exon 22546969 22547127 22546969 ID=MS.gene72456.t1.exon4;Parent=MS.gene72456.t1
chr2.3 CDS 22546969 22547127 22546969 ID=cds.MS.gene72456.t1;Parent=MS.gene72456.t1
chr2.3 exon 22547705 22547972 22547705 ID=MS.gene72456.t1.exon5;Parent=MS.gene72456.t1
chr2.3 CDS 22547705 22547972 22547705 ID=cds.MS.gene72456.t1;Parent=MS.gene72456.t1
Gene Sequence

>MS.gene72456

ATGTCACTCACATTCCACCAACTATGTTCTCTGCTACTAGCTTCAGCATTTCTATGGACACTACTACAACCTTCTCTACAACAACCATGTGATTACTCTCAAGGCAACTGGTTCATTGATGATGATTCTACTACCTTCTCCTACCCTCTCTATGATGCTTCAAAGGACTGTCCTTTCATTGGTCAAGGTTTTGATTGTCTTGGAAATGGTAGAGCTGATAAAGATTATATCAAGTATAGATGGAAACCCTTCAGTTGTAATCTTCCAAGGTAGTTATATTTGAGTTAAAGTTTTGTTTTTTGGAATGTATCTTCAAATGGGTAGTGACCAAAGTTCTTGTTTTTATTTTCAGTTTTTATGTTTTTTTGTTGTTGTTGTTAGTATGATTTTTGTTTGAAACTATAGGAGAAAGTGATAAGAAAAATATAAAAAAATTAATGAGTTTGATCGACATGTGATATATGATTGAACATTATGACTTTGTTTGATCAATGTTGTCGACCTCATTTAGTGAGACAAAACTTTGTTGTTGTTATTGTTGTTGGTGTATGATATTGGTATGAAGTTTCAACCGATTTGTGGATGATTGACTATGTTTTTCGAGAACATAGTTGACTATTTTTAGTGGGATCTCCATTTTCAACTATGTTTTTGAGACCGGGATCTGAGTCTGATTACACTTGGACTAATGTAATGTAATGTTGTTAATTCAATGTTTATGTTTGATCGGCAACAAAAGTTAGCCACATTTTTTTCCATCACAAGGCTCAAACTCGAGACATTGTTTAAACTCGACACATTGTAAGGATCTGAGTCTAGTTAGACTCAGATGAATGTAATGTTAGTAATTCAATGCTTATGTTGGCTAGAGACCTTCCAGTGTGCTACTCTCAACTCTCAAGGTCTCAATTCAATTCTCCTTAGCATCAATTTCGGTGGGCTAGTTTAGCTTCTTCAAAAAAAAAAATGTTTCTGTTTACTTAGCAAAAAAGTAAACATTCTTTTGCTTACATAATGACTTTTGTTAACCACGTTGTTTCCATCACAAGAATCAAACTCGAGACTTTGTTTAAGAAATCTGAGTCTAGTTAGACTTAGATGAATGTAATGTGGATAATACAATTTTCATGTTTGATTAATAAAATGAGAACTTATTTTTTAGCTTCTCTAATGGTTTTTGTTAACAGGTTTGATGGTGGGAAGTTTTTGGAAAGATACAAAGGAAAGAAGATAATGTTTGTGGGAGATTCCATAAGCAATAACATGTGGCAATCACTCACTTGTTTGCTTCACATTGCAATCCCAAATTCAACTTATACCTTAACAAAACAAACAAAAGAACTCACTGTGTTCTCAATTCCGGTACTATTCTTTAGCTTATATTTGCATTACAAAAAAATGTGTTTTTTAATTGTTTGATTAGTTAAATGATTTTGTCTAATGTGGTTTTTTGGGGGGTGTCATAGGAATATGAAGCTTCAATCATGTGGTTGAAAAATGGGTTCTTAGTAGATTTGGTTCATGATAAAGAAAAGGGAAGAATTTTGAGGTTGGACACAATTAGCTCAGGAAATCAATGGAAAGGTTTTGATGTTTTGATTTTCAATACATATCATTGGTGGACTCATACTGGAAATTCTCAAACGTATTGATTTCTTTGAACTCAAATTTTGGTATTTTTATGTGAGGAATTTGAGCATGTGGTTAATGGTTTTTTATTTAATTGTTTTCACAGATGGGATTATTTTCAAGTTGGGAATGAATTAATTAAGGAGATGGATCATTTGGAAGCTTTTAAGATTGGATTAAGCACTTGGGCCAAATGGGTTGATTCCAACATTGAACCCTCAAAGACTAGAGTTTTGTTTCAAGGAATAGCTGCTTCTCATGTTGAGTAAGTTTAACTGTTTCTTCCACTCAATGAATGTTTGGATTTTCAAAGTATGTGCTAACTAAATGTTGATGTGTGCAACTTAACAAACTCAATTCTGCATGAATTATATATAAGTAAGGTTTTATGCAGTCATGGATGTGGTCGCATTACAATCATTGATATTGCTGAAAAGTTCAGTCAAATGAAGTCTCACTTGTGGTCTATGAAGCACTATCATAGATGCATACACTAGATACGACACTGATAATTGTTTGAAATTTGAAAAAAATGAATGTAAATTATTTGTCATAGCTTCGTGTCGGTGTCTGACACTTAATAGACACCGGACCGGACACGCTTTTGATCTGAAGTCCCAGTGTTACACAGATTGCAACGTATTCCACGGCTCAGATTCTAGTTGTGTGACCTTTTGTTAAAATCTTGATTATAAGTATTATGTGAAGAGAGATCATAGAAAGACATTTAAGACACTGAATTTTATTATGATGAAATGGTAATATTGATTATGAAAGTATATACTCAAAGACTCACTCTTTTTTCCCTTTTCTTCTTTTCCTGAATGACATGAAAATTCTAGTGGAAAAGGGTGCTTGAGACAAACTAAACCAGATCAAGGACCAATGCCACCTTATCCTGGTGTAGACACTGTGAAGAGCATCATAAGTAAAATGGAGAAGCCAGTACAATTGCTTGACATCACTTTGCTAACACAGTTAAGGAGGGATGGTCACCCGTCTATCTACACCGGCCGTGGCGAAAGTTATGTCGACTGTAGTCACTGGTGTCTTGCTGGTGTTCCTGATACTTGGAATGAAATGTTGTATGCTGCTCTTCTTGAAGATTAG
Protein sequence

>MS.gene72456.t1

MSLTFHQLCSLLLASAFLWTLLQPSLQQPCDYSQGNWFIDDDSTTFSYPLYDASKDCPFIGQGFDCLGNGRADKDYIKYRWKPFSCNLPRFDGGKFLERYKGKKIMFVGDSISNNMWQSLTCLLHIAIPNSTYTLTKQTKELTVFSIPEYEASIMWLKNGFLVDLVHDKEKGRILRLDTISSGNQWKGFDVLIFNTYHWWTHTGNSQTWDYFQVGNELIKEMDHLEAFKIGLSTWAKWVDSNIEPSKTRVLFQGIAASHVDGKGCLRQTKPDQGPMPPYPGVDTVKSIISKMEKPVQLLDITLLTQLRRDGHPSIYTGRGESYVDCSHWCLAGVPDTWNEMLYAALLED