AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31379

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31379.t1 MTR_2g091175 97.283 368 7 1 1 365 1 368 0.0 749
MS.gene31379.t1 MTR_4g079700 75.000 360 85 1 4 363 9 363 0.0 584
MS.gene31379.t1 MTR_2g015720 59.836 366 136 5 1 363 1 358 8.34e-160 452
MS.gene31379.t1 MTR_4g087730 55.890 365 158 2 1 364 1 363 1.17e-152 434
MS.gene31379.t1 MTR_4g088030 55.464 366 146 3 1 364 1 351 9.10e-149 424
MS.gene31379.t1 MTR_3g049330 54.829 321 142 2 47 364 31 351 2.25e-133 385
MS.gene31379.t1 MTR_2g073510 55.799 319 137 3 46 360 32 350 2.90e-131 380
MS.gene31379.t1 MTR_3g021060 52.905 327 153 1 38 363 37 363 3.17e-130 377
MS.gene31379.t1 MTR_3g035830 50.746 335 164 1 30 363 14 348 1.13e-127 370
MS.gene31379.t1 MTR_3g021090 50.755 331 162 1 30 359 14 344 3.52e-124 364
MS.gene31379.t1 MTR_5g034810 51.497 334 156 4 35 364 22 353 1.13e-122 358
MS.gene31379.t1 MTR_3g464570 44.800 375 190 4 7 364 3 377 6.58e-116 342
MS.gene31379.t1 MTR_5g071350 44.236 373 167 5 17 364 44 400 4.90e-115 340
MS.gene31379.t1 MTR_2g073500 45.289 329 158 8 47 364 29 346 4.04e-101 303
MS.gene31379.t1 MTR_1g050448 42.609 345 174 8 34 363 40 375 5.02e-96 291
MS.gene31379.t1 MTR_1g111010 45.897 329 157 10 44 365 29 343 6.42e-93 282
MS.gene31379.t1 MTR_1g112210 38.329 347 193 6 31 363 43 382 6.71e-92 280
MS.gene31379.t1 MTR_1g112210 39.429 350 172 9 31 363 43 369 2.46e-91 278
MS.gene31379.t1 MTR_0136s0010 38.400 375 204 6 15 364 138 510 6.01e-90 280
MS.gene31379.t1 MTR_7g093450 40.233 343 180 6 46 364 247 588 2.05e-89 281
MS.gene31379.t1 MTR_3g464570 47.407 270 125 4 112 364 4 273 4.48e-84 256
MS.gene31379.t1 MTR_4g126950 37.318 343 186 6 46 363 108 446 4.94e-84 263
MS.gene31379.t1 MTR_5g033800 35.616 365 211 6 23 364 121 484 2.36e-83 262
MS.gene31379.t1 MTR_4g121560 38.710 372 197 10 23 364 84 454 1.07e-81 257
MS.gene31379.t1 MTR_3g070900 39.017 346 184 9 47 365 106 451 2.08e-81 256
MS.gene31379.t1 MTR_7g109550 39.759 332 176 8 47 359 137 463 3.48e-80 253
MS.gene31379.t1 MTR_8g069790 37.356 348 188 6 43 364 174 517 6.82e-80 254
MS.gene31379.t1 MTR_5g082880 38.109 349 186 10 47 365 165 513 1.62e-78 250
MS.gene31379.t1 MTR_5g059400 36.827 353 193 8 39 364 167 516 1.66e-78 250
MS.gene31379.t1 MTR_1g116130 36.827 353 193 8 39 364 167 516 1.66e-78 250
MS.gene31379.t1 MTR_3g071650 34.085 399 227 10 2 364 33 431 2.00e-75 239
MS.gene31379.t1 MTR_5g083260 37.318 343 190 7 47 364 83 425 4.02e-75 239
MS.gene31379.t1 MTR_4g016940 34.293 382 209 10 24 363 46 427 3.91e-74 236
MS.gene31379.t1 MTR_1g058380 35.567 388 216 10 4 364 15 395 5.60e-73 233
MS.gene31379.t1 MTR_8g463790 35.613 351 196 9 40 364 64 410 6.50e-71 228
MS.gene31379.t1 MTR_2g022560 37.059 340 188 7 47 361 97 435 1.02e-68 223
MS.gene31379.t1 MTR_7g094750 36.812 345 189 8 47 364 91 433 3.54e-68 221
MS.gene31379.t1 MTR_4g014430 35.345 348 199 9 36 359 97 442 8.77e-68 220
MS.gene31379.t1 MTR_4g014440 32.665 349 206 8 45 364 60 408 1.18e-66 216
MS.gene31379.t1 MTR_7g070060 31.714 391 231 10 4 365 28 411 2.17e-66 216
MS.gene31379.t1 MTR_7g068920 35.838 346 195 9 44 363 77 421 5.09e-66 215
MS.gene31379.t1 MTR_3g090120 36.202 337 191 7 47 364 129 460 3.96e-62 206
MS.gene31379.t1 MTR_5g033800 35.135 259 156 4 23 269 121 379 1.07e-54 184
MS.gene31379.t1 MTR_8g093530 30.117 342 219 6 38 364 81 417 3.72e-53 182
MS.gene31379.t1 MTR_5g069320 33.224 304 143 9 74 356 122 386 1.63e-45 160
MS.gene31379.t1 MTR_7g094750 35.294 238 140 3 47 270 91 328 5.97e-45 157
MS.gene31379.t1 MTR_7g070060 30.182 275 175 5 4 268 28 295 1.13e-44 156
MS.gene31379.t1 MTR_7g070060 30.195 308 186 9 87 365 2 309 8.33e-42 149
MS.gene31379.t1 MTR_6g016880 28.753 393 232 11 4 363 42 419 3.11e-39 144
MS.gene31379.t1 MTR_5g035080 30.102 392 219 17 9 359 39 416 4.02e-38 142
MS.gene31379.t1 MTR_1g076690 28.792 389 230 13 12 359 184 566 6.56e-38 143
MS.gene31379.t1 MTR_5g035090 31.339 351 195 16 46 359 82 423 7.59e-38 141
MS.gene31379.t1 MTR_6g061710 27.632 380 233 11 16 359 137 510 2.24e-36 138
MS.gene31379.t1 MTR_6g016875 28.499 393 234 14 3 359 23 404 1.31e-34 132
MS.gene31379.t1 MTR_7g019120 28.161 348 207 9 46 359 78 416 1.35e-34 132
MS.gene31379.t1 MTR_6g016240 30.725 345 204 12 47 359 71 412 3.17e-34 131
MS.gene31379.t1 MTR_8g093870 31.389 360 206 17 35 363 123 472 3.89e-34 131
MS.gene31379.t1 MTR_5g035150 30.636 346 204 11 47 359 85 427 7.69e-34 130
MS.gene31379.t1 MTR_8g027900 26.902 368 221 10 34 363 198 555 6.39e-32 126
MS.gene31379.t1 MTR_2g016060 28.940 349 203 13 48 359 56 396 7.91e-32 124
MS.gene31379.t1 MTR_5g035070 29.480 346 208 12 47 359 87 429 1.26e-31 124
MS.gene31379.t1 MTR_7g033230 28.532 361 205 15 40 359 257 605 9.50e-31 123
MS.gene31379.t1 MTR_7g033230 28.532 361 205 15 40 359 327 675 1.28e-30 123
MS.gene31379.t1 MTR_4g092830 27.666 347 211 13 47 359 48 388 8.51e-30 119
MS.gene31379.t1 MTR_8g104970 27.397 365 218 13 33 359 106 461 2.80e-29 118
MS.gene31379.t1 MTR_3g021150 44.737 114 62 1 231 343 1 114 4.40e-29 109
MS.gene31379.t1 MTR_4g125060 29.395 347 208 15 47 363 132 471 5.92e-29 117
MS.gene31379.t1 MTR_4g125060 29.395 347 208 15 47 363 171 510 6.66e-29 117
MS.gene31379.t1 MTR_4g125060 29.395 347 208 15 47 363 200 539 6.70e-29 117
MS.gene31379.t1 MTR_3g109840 27.342 395 239 14 3 359 27 411 8.16e-29 116
MS.gene31379.t1 MTR_6g007777 28.788 396 218 18 14 363 80 457 2.76e-28 115
MS.gene31379.t1 MTR_8g099300 27.066 351 215 13 44 359 71 415 1.48e-27 112
MS.gene31379.t1 MTR_7g019140 44.444 90 50 0 46 135 78 167 4.84e-22 92.0
MS.gene31379.t1 MTR_5g035120 39.048 105 64 0 37 141 65 169 6.63e-19 83.6
MS.gene31379.t1 MTR_2g022580 32.773 119 66 3 107 211 2 120 2.54e-12 63.9
MS.gene31379.t1 MTR_3g021080 31.683 101 49 2 265 364 99 180 5.61e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31379.t1 AT2G42570 65.988 344 110 3 28 364 22 365 5.50e-180 504
MS.gene31379.t1 AT2G31110 62.928 321 118 1 44 363 42 362 8.48e-166 468
MS.gene31379.t1 AT1G29050 59.882 339 134 2 27 363 40 378 1.16e-157 447
MS.gene31379.t1 AT2G34070 57.681 345 138 4 27 363 39 383 2.30e-150 429
MS.gene31379.t1 AT3G14850 55.108 323 141 2 42 360 29 351 3.96e-131 379
MS.gene31379.t1 AT1G78710 49.254 335 164 4 34 364 25 357 9.44e-122 356
MS.gene31379.t1 AT2G30900 50.000 324 156 4 47 364 43 366 1.22e-119 350
MS.gene31379.t1 AT2G34070 60.643 249 96 2 117 363 1 249 1.80e-111 325
MS.gene31379.t1 AT5G58600 47.619 336 158 5 47 364 66 401 1.39e-110 329
MS.gene31379.t1 AT2G31110 61.927 218 83 0 44 261 42 259 8.11e-108 317
MS.gene31379.t1 AT2G30010 45.322 342 163 5 47 364 99 440 3.01e-105 317
MS.gene31379.t1 AT2G30010 45.322 342 163 5 47 364 56 397 3.28e-105 315
MS.gene31379.t1 AT2G31110 63.768 207 74 1 158 363 8 214 4.11e-104 305
MS.gene31379.t1 AT3G14850 55.242 248 107 2 117 360 1 248 2.20e-95 285
MS.gene31379.t1 AT3G54260 42.515 334 167 6 47 364 51 375 7.46e-92 280
MS.gene31379.t1 AT5G06700 42.274 343 171 7 47 364 255 595 4.12e-91 285
MS.gene31379.t1 AT3G12060 39.678 373 197 7 19 364 165 536 1.77e-89 280
MS.gene31379.t1 AT1G78710 39.665 358 151 6 11 364 1 297 3.96e-88 268
MS.gene31379.t1 AT2G30900 50.424 236 109 3 47 280 43 272 1.15e-84 258
MS.gene31379.t1 AT3G62390 39.650 343 186 7 41 364 132 472 3.23e-82 258
MS.gene31379.t1 AT5G58600 51.786 224 106 2 47 268 66 289 5.99e-82 252
MS.gene31379.t1 AT5G20590 38.571 350 184 9 41 364 136 480 8.74e-82 258
MS.gene31379.t1 AT5G49340 37.960 353 190 8 39 364 92 442 1.11e-80 254
MS.gene31379.t1 AT1G60790 38.211 369 194 10 26 364 169 533 3.83e-80 255
MS.gene31379.t1 AT3G55990 36.565 361 199 10 35 365 125 485 3.23e-77 246
MS.gene31379.t1 AT2G40150 35.977 353 193 8 46 365 70 422 8.64e-76 240
MS.gene31379.t1 AT3G11030 38.000 350 179 10 47 364 106 449 8.90e-75 238
MS.gene31379.t1 AT2G40160 35.817 349 192 9 47 364 77 424 3.37e-74 237
MS.gene31379.t1 AT2G40160 35.569 343 191 8 47 359 77 419 4.65e-74 236
MS.gene31379.t1 AT1G73140 36.441 354 195 8 37 364 57 406 4.32e-73 233
MS.gene31379.t1 AT5G19160 36.119 371 194 10 24 361 82 442 1.73e-72 233
MS.gene31379.t1 AT5G01360 35.756 344 195 7 46 363 35 378 4.83e-72 229
MS.gene31379.t1 AT5G01360 35.756 344 195 7 46 363 91 434 2.46e-71 229
MS.gene31379.t1 AT5G01620 34.402 343 198 8 42 359 105 445 1.09e-70 228
MS.gene31379.t1 AT5G01620 34.402 343 198 8 42 359 105 445 1.09e-70 228
MS.gene31379.t1 AT5G01620 34.402 343 198 8 42 359 113 453 1.11e-70 228
MS.gene31379.t1 AT2G40320 35.632 348 199 8 42 364 76 423 7.16e-70 225
MS.gene31379.t1 AT3G06080 36.550 342 189 7 47 361 103 443 1.39e-69 226
MS.gene31379.t1 AT5G06230 35.294 357 202 7 36 364 10 365 1.11e-67 218
MS.gene31379.t1 AT5G06230 35.294 357 202 7 36 364 51 406 2.13e-67 218
MS.gene31379.t1 AT3G11570 37.538 325 180 7 63 365 98 421 5.45e-67 218
MS.gene31379.t1 AT2G38320 33.333 351 204 9 44 364 55 405 3.30e-64 210
MS.gene31379.t1 AT1G48880 33.973 365 194 8 21 364 102 440 4.30e-62 205
MS.gene31379.t1 AT3G62390 39.095 243 136 4 41 271 132 374 4.75e-57 191
MS.gene31379.t1 AT5G20590 39.431 246 128 7 41 269 136 377 1.54e-55 186
MS.gene31379.t1 AT5G19160 36.538 260 145 4 24 269 82 335 2.91e-53 180
MS.gene31379.t1 AT2G38320 31.056 322 194 7 71 364 1 322 3.83e-53 179
MS.gene31379.t1 AT2G40160 35.470 234 139 4 47 268 77 310 3.74e-50 171
MS.gene31379.t1 AT3G06080 37.131 237 135 3 47 269 103 339 3.90e-49 169
MS.gene31379.t1 AT5G06230 35.433 254 144 4 36 269 51 304 7.50e-46 159
MS.gene31379.t1 AT1G70230 29.513 349 198 11 47 363 79 411 6.77e-40 146
MS.gene31379.t1 AT2G14530 29.049 389 240 12 7 363 22 406 7.89e-40 146
MS.gene31379.t1 AT5G51640 28.307 378 223 11 23 359 114 484 9.31e-40 147
MS.gene31379.t1 AT4G25360 28.165 387 216 12 19 359 150 520 2.25e-37 141
MS.gene31379.t1 AT4G25360 28.165 387 216 12 19 359 150 520 2.25e-37 141
MS.gene31379.t1 AT5G64020 29.494 356 208 12 47 363 57 408 6.19e-35 132
MS.gene31379.t1 AT5G64470 26.744 344 223 10 47 364 55 395 1.58e-34 131
MS.gene31379.t1 AT1G01430 28.609 381 223 12 28 365 75 449 1.29e-33 130
MS.gene31379.t1 AT5G64470 26.857 350 221 11 47 364 55 401 3.63e-33 127
MS.gene31379.t1 AT5G15890 28.065 367 219 12 25 359 165 518 1.45e-32 128
MS.gene31379.t1 AT2G37720 30.939 362 183 14 47 363 143 482 3.86e-32 126
MS.gene31379.t1 AT5G15900 26.934 349 217 11 47 363 67 409 3.89e-32 125
MS.gene31379.t1 AT5G15900 26.934 349 217 11 47 363 86 428 5.06e-32 125
MS.gene31379.t1 AT5G20680 29.111 371 222 14 26 363 95 457 7.40e-32 125
MS.gene31379.t1 AT5G20680 29.111 371 222 14 26 363 171 533 1.53e-31 125
MS.gene31379.t1 AT5G20680 29.111 371 222 14 26 363 189 551 2.07e-31 125
MS.gene31379.t1 AT5G20680 29.111 371 222 14 26 363 189 551 2.07e-31 125
MS.gene31379.t1 AT5G20680 29.111 371 222 14 26 363 189 551 2.07e-31 125
MS.gene31379.t1 AT5G20680 29.111 371 222 14 26 363 189 551 2.07e-31 125
MS.gene31379.t1 AT3G28150 30.966 352 199 16 46 363 69 410 3.04e-31 122
MS.gene31379.t1 AT3G02440 26.513 347 212 12 47 359 127 464 5.29e-31 123
MS.gene31379.t1 AT3G02440 26.513 347 212 12 47 359 135 472 5.30e-31 123
MS.gene31379.t1 AT4G11090 25.679 405 243 15 8 365 35 428 1.29e-28 115
MS.gene31379.t1 AT4G01080 27.624 362 215 12 33 359 77 426 7.95e-28 113
MS.gene31379.t1 AT4G23790 25.683 366 213 14 46 365 78 430 6.02e-23 99.8
MS.gene31379.t1 AT5G64470 26.378 254 166 8 47 282 55 305 5.33e-21 92.8
MS.gene31379.t1 AT5G64470 26.378 254 166 8 47 282 55 305 7.54e-21 92.4

Find 74 sgRNAs with CRISPR-Local

Find 275 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCATGCAGCTCTCTT+TGG 0.210145 2.4:+9178909 MS.gene31379:CDS
TCTATTCCGCACACCATATT+TGG 0.219542 2.4:+9177800 MS.gene31379:CDS
TATCTCCACAATAACATTTC+AGG 0.240854 2.4:+9174627 MS.gene31379:CDS
AGAGAAACACAACCATTCTT+TGG 0.302957 2.4:+9178684 MS.gene31379:CDS
CGGTCAAGGTCTACCAAATA+TGG 0.307611 2.4:-9177813 None:intergenic
GTTTGACAACTTGGGCTAGA+TGG 0.309964 2.4:+9178115 MS.gene31379:CDS
TAGCATGTATGATTCATTCT+TGG 0.316438 2.4:+9174566 MS.gene31379:CDS
TGTAGTCATTGGTGTCTTCC+AGG 0.323483 2.4:+9178864 MS.gene31379:CDS
GTGTGCGGAATAGAAATAAT+TGG 0.347634 2.4:-9177791 None:intergenic
CAAGCCATTGGTGTTCCTGC+TGG 0.351195 2.4:-9178717 None:intergenic
AGAAGAACATTCCAAGTATC+AGG 0.357411 2.4:-9178891 None:intergenic
TCTACAAAGGTTTGACAACT+TGG 0.371927 2.4:+9178106 MS.gene31379:CDS
TTCCAGGACTTCCTGATACT+TGG 0.372897 2.4:+9178880 MS.gene31379:CDS
CAATTCAATTGTCAAAAGTA+TGG 0.391925 2.4:+9174154 MS.gene31379:CDS
TATTTCTGATACTGAGTATC+AGG 0.397938 2.4:-9174181 None:intergenic
ACCTTGACCGTGAGAACATT+GGG 0.399023 2.4:+9177826 MS.gene31379:CDS
AGCATGTATGATTCATTCTT+GGG 0.407416 2.4:+9174567 MS.gene31379:CDS
AAGAAGACCTTGGTTTGAGC+TGG 0.426020 2.4:-9178156 None:intergenic
GTAACTGGTTTCGTGGCAAA+TGG 0.428187 2.4:+9174074 MS.gene31379:CDS
CTCAGTATCAGAAATATAGA+TGG 0.444563 2.4:+9174188 MS.gene31379:CDS
TGCCAACAGATTGTAGTCAT+TGG 0.444942 2.4:+9178853 MS.gene31379:CDS
AGCAGGAACACCAATGGCTT+GGG 0.461353 2.4:+9178719 MS.gene31379:CDS
AACTCGCTGGAAGATGTAAC+TGG 0.464742 2.4:+9174059 MS.gene31379:CDS
TACATGCTAGTGAATTGAAC+TGG 0.471439 2.4:-9174552 None:intergenic
TTTGACAACTTGGGCTAGAT+GGG 0.479154 2.4:+9178116 MS.gene31379:CDS
TTCACTATCACCCAAGCCAT+TGG 0.480458 2.4:-9178729 None:intergenic
ATAACACTACTATACCCTTC+TGG 0.481855 2.4:-9178831 None:intergenic
CATTGGGCGTGTTCTAAAGT+TGG 0.484902 2.4:+9177842 MS.gene31379:CDS
ATTGAATTGTGGATCTATGA+AGG 0.486450 2.4:-9174140 None:intergenic
TTCCAAGTATCAGGAAGTCC+TGG 0.489986 2.4:-9178882 None:intergenic
CAGCAGGAACACCAATGGCT+TGG 0.502272 2.4:+9178718 MS.gene31379:CDS
AAGCCATTGGTGTTCCTGCT+GGG 0.502968 2.4:-9178716 None:intergenic
CGGTTGTGCATTTCCAGTGT+GGG 0.509875 2.4:-9177934 None:intergenic
ATGGAGAGGAATGGATGTAT+TGG 0.512382 2.4:+9177887 MS.gene31379:CDS
CTTTGTAGAACGCAATGAAA+CGG 0.518428 2.4:-9178092 None:intergenic
TAACTGGTTTCGTGGCAAAT+GGG 0.526407 2.4:+9174075 MS.gene31379:CDS
CTCATTACTAGTACTGGAAA+CGG 0.528540 2.4:-9174032 None:intergenic
CGTTTCATTGCGTTCTACAA+AGG 0.529142 2.4:+9178093 MS.gene31379:CDS
GATAGCACTTTGCTTTGAGA+AGG 0.530854 2.4:-9174608 None:intergenic
CTACAAAGGTTTGACAACTT+GGG 0.543154 2.4:+9178107 MS.gene31379:CDS
TGGCATTGGTGGACCCACAC+TGG 0.546946 2.4:+9177921 MS.gene31379:CDS
GACCTTGACCGTGAGAACAT+TGG 0.550954 2.4:+9177825 MS.gene31379:CDS
TCTATGAAGGGACAAGAAGA+AGG 0.551169 2.4:-9174127 None:intergenic
GGTAGTTGTTGCTGTTGTGT+TGG 0.556553 2.4:-9174011 None:intergenic
GGAAGATGTAACTGGTTTCG+TGG 0.558355 2.4:+9174067 MS.gene31379:CDS
GAAAAGATGCACATCCAGAA+GGG 0.559145 2.4:+9178817 MS.gene31379:CDS
TTTGGTTTGAAGTACCCAGC+AGG 0.566621 2.4:+9178702 MS.gene31379:CDS
GGATCATAGAGAGGATACGA+AGG 0.570131 2.4:-9174106 None:intergenic
GGGTACTTCAAACCAAAGAA+TGG 0.571214 2.4:-9178696 None:intergenic
CTTTCACTTGTTCTATACCA+AGG 0.575010 2.4:+9174215 MS.gene31379:CDS
ACTAACCTGAAATGTTATTG+TGG 0.576103 2.4:-9174632 None:intergenic
CAATAAAGAGTGGTAATGCA+TGG 0.581612 2.4:+9177868 MS.gene31379:CDS
ACGGTTGTGCATTTCCAGTG+TGG 0.583332 2.4:-9177935 None:intergenic
AGAAAAGATGCACATCCAGA+AGG 0.583703 2.4:+9178816 MS.gene31379:CDS
TGGTAATGCATGGAGAGGAA+TGG 0.587257 2.4:+9177878 MS.gene31379:CDS
CACCAATGACTACAATCTGT+TGG 0.588126 2.4:-9178855 None:intergenic
GCCCAATGTTCTCACGGTCA+AGG 0.590015 2.4:-9177827 None:intergenic
AAGAGTGGTAATGCATGGAG+AGG 0.592479 2.4:+9177873 MS.gene31379:CDS
CAGATGGGATTATATTCAAG+AGG 0.594517 2.4:+9178044 MS.gene31379:intron
AGTAATGAGAGAAAACTCGC+TGG 0.595143 2.4:+9174046 MS.gene31379:CDS
ACAACTTACTCGTAATGCAC+AGG 0.597709 2.4:-9178192 None:intergenic
TTGAATTGTGGATCTATGAA+GGG 0.607685 2.4:-9174139 None:intergenic
TGTAAATCCAGCTCAAACCA+AGG 0.609530 2.4:+9178149 MS.gene31379:CDS
CTTGGTATAGAACAAGTGAA+AGG 0.623660 2.4:-9174214 None:intergenic
GAACACGCCCAATGTTCTCA+CGG 0.633134 2.4:-9177833 None:intergenic
TTATATTCAAGAGGGTGGCA+AGG 0.635985 2.4:+9178053 MS.gene31379:CDS
TGGGATTATATTCAAGAGGG+TGG 0.638991 2.4:+9178048 MS.gene31379:CDS
CAAGAAGAAGGATCATAGAG+AGG 0.640153 2.4:-9174115 None:intergenic
GTACCCAGCAGGAACACCAA+TGG 0.661011 2.4:+9178713 MS.gene31379:CDS
AGATGGGATTATATTCAAGA+GGG 0.662305 2.4:+9178045 MS.gene31379:intron
GGTCTACCAAATATGGTGTG+CGG 0.672109 2.4:-9177806 None:intergenic
GATCATAGAGAGGATACGAA+GGG 0.672935 2.4:-9174105 None:intergenic
GTCACATCTAGAAAATACAC+TGG 0.675733 2.4:-9178777 None:intergenic
AAGTTGGACTCAATAAAGAG+TGG 0.679146 2.4:+9177858 MS.gene31379:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTATATATTTATTCATTAT+AGG + chr2.4:9177152-9177171 MS.gene31379:intron 10.0%
!! TTTAAAAAATACATTTATTG+GGG + chr2.4:9175170-9175189 MS.gene31379:intron 10.0%
!! TTTATCAACTATATTTATAA+TGG + chr2.4:9178602-9178621 MS.gene31379:intron 10.0%
!!! TTATTATTTGAATTATTTTC+AGG + chr2.4:9177758-9177777 MS.gene31379:intron 10.0%
!! AATATAAATTACTAGAAGAA+AGG - chr2.4:9177430-9177449 None:intergenic 15.0%
!! ACAAAAAATAAAATTTGAAG+TGG + chr2.4:9177091-9177110 MS.gene31379:intron 15.0%
!! AGAATAATACAATATTAGTA+TGG + chr2.4:9176551-9176570 MS.gene31379:intron 15.0%
!! ATAAAATTTAGTCTAGTAAT+TGG - chr2.4:9178587-9178606 None:intergenic 15.0%
!! ATGTACAATTATTATATGTA+AGG + chr2.4:9177584-9177603 MS.gene31379:intron 15.0%
!! GTAAATAAATTATATGTTAG+TGG + chr2.4:9176525-9176544 MS.gene31379:intron 15.0%
!! TTAAAAAATACATTTATTGG+GGG + chr2.4:9175171-9175190 MS.gene31379:intron 15.0%
!!! AACTTTTATAAATCATTTGA+CGG - chr2.4:9177637-9177656 None:intergenic 15.0%
!!! AATGTTTTTTTACAGAATTA+CGG + chr2.4:9178263-9178282 MS.gene31379:intron 15.0%
!!! ATTTTTATTATTTTCCATGT+TGG + chr2.4:9174913-9174932 MS.gene31379:intron 15.0%
!!! CAAAAAATAAAATTTGAAGT+GGG + chr2.4:9177092-9177111 MS.gene31379:intron 15.0%
!!! CATAATTTAAGTTTACATAT+AGG + chr2.4:9175719-9175738 MS.gene31379:intron 15.0%
!!! GATCATGAATATAATTTTAT+TGG - chr2.4:9176733-9176752 None:intergenic 15.0%
!!! TTCAATACAAATTTCATTTT+TGG - chr2.4:9176404-9176423 None:intergenic 15.0%
!!! TTTGATACTTTTGAATTTAA+TGG + chr2.4:9174694-9174713 MS.gene31379:intron 15.0%
!! AAAGAAATCATTAGAATGTT+TGG + chr2.4:9178388-9178407 MS.gene31379:intron 20.0%
!! ATTACTATCTATTATTACCA+TGG - chr2.4:9175096-9175115 None:intergenic 20.0%
!! ATTTGAAGAAAAACAAAACA+TGG - chr2.4:9174839-9174858 None:intergenic 20.0%
!! CATAATCAATTCTGTTATTA+AGG + chr2.4:9177982-9178001 MS.gene31379:intron 20.0%
!! GCTATTCACATTAAAAAAAT+AGG + chr2.4:9177037-9177056 MS.gene31379:intron 20.0%
!! GTACTACAATTTAATTCATA+TGG - chr2.4:9177118-9177137 None:intergenic 20.0%
!! GTATGATATTTGGTAAATTA+GGG + chr2.4:9178626-9178645 MS.gene31379:intron 20.0%
!! TAAAAAAAGATACAAACTAG+TGG + chr2.4:9176574-9176593 MS.gene31379:intron 20.0%
!! TAAAAAATACATTTATTGGG+GGG + chr2.4:9175172-9175191 MS.gene31379:intron 20.0%
!! TATGATATTTGGTAAATTAG+GGG + chr2.4:9178627-9178646 MS.gene31379:intron 20.0%
!! TCAGAATGATAATAAAAGAA+GGG - chr2.4:9175430-9175449 None:intergenic 20.0%
!! TCCTAAAGTTTCTATATAAA+GGG - chr2.4:9176775-9176794 None:intergenic 20.0%
!! TGATCATTACAGTTTAAAAA+GGG - chr2.4:9176927-9176946 None:intergenic 20.0%
!! TGTATGATATTTGGTAAATT+AGG + chr2.4:9178625-9178644 MS.gene31379:intron 20.0%
!! TTATAATGGTGTATGATATT+TGG + chr2.4:9178616-9178635 MS.gene31379:intron 20.0%
!! TTATGAAAAAACTATTACCA+TGG - chr2.4:9175067-9175086 None:intergenic 20.0%
!! TTCAGAATGATAATAAAAGA+AGG - chr2.4:9175431-9175450 None:intergenic 20.0%
!! TTCCTAAAGTTTCTATATAA+AGG - chr2.4:9176776-9176795 None:intergenic 20.0%
!! TTCTAGTAATTTATATTCCA+TGG + chr2.4:9177433-9177452 MS.gene31379:intron 20.0%
!! TTGATCATTACAGTTTAAAA+AGG - chr2.4:9176928-9176947 None:intergenic 20.0%
!! TTTAAATTTCAGTAGAATCA+GGG + chr2.4:9178300-9178319 MS.gene31379:intron 20.0%
!! TTTGAAGAAAAACAAAACAT+GGG - chr2.4:9174838-9174857 None:intergenic 20.0%
!!! ATTAGCAATAATTAGTGTTT+TGG + chr2.4:9176807-9176826 MS.gene31379:intron 20.0%
!!! TTGAATTATTTTCAGGATTA+TGG + chr2.4:9177765-9177784 MS.gene31379:intron 20.0%
!!! TTTTAAATTTCAGTAGAATC+AGG + chr2.4:9178299-9178318 MS.gene31379:intron 20.0%
!!! TTTTAGAAATTTAGATGAAG+AGG - chr2.4:9175406-9175425 None:intergenic 20.0%
!!! TTTTTTCATAAAAAATGCCA+TGG + chr2.4:9175076-9175095 MS.gene31379:intron 20.0%
! AAAAGATAATCCCTTCAAAA+TGG + chr2.4:9177275-9177294 MS.gene31379:intron 25.0%
! AACAGAATTGATTATGATCA+TGG - chr2.4:9177978-9177997 None:intergenic 25.0%
! AATCAATTCTGTTATTAAGG+AGG + chr2.4:9177985-9178004 MS.gene31379:intron 25.0%
! ACTCAATAAAGTTTCCAATT+AGG - chr2.4:9174799-9174818 None:intergenic 25.0%
! AGTTTCTATATAAAGGGAAA+AGG - chr2.4:9176769-9176788 None:intergenic 25.0%
! ATAGAACACCAAATATAAGA+CGG - chr2.4:9176359-9176378 None:intergenic 25.0%
! ATGATATTTGGTAAATTAGG+GGG + chr2.4:9178628-9178647 MS.gene31379:intron 25.0%
! CAATTCAATTGTCAAAAGTA+TGG + chr2.4:9174154-9174173 MS.gene31379:CDS 25.0%
! GATCATTACAGTTTAAAAAG+GGG - chr2.4:9176926-9176945 None:intergenic 25.0%
! GGAAAAAAGATTATGTTTGT+TGG + chr2.4:9174511-9174530 MS.gene31379:CDS 25.0%
! GTAAATGATAGTAATAACCA+TGG - chr2.4:9175039-9175058 None:intergenic 25.0%
! TAAGTAGCTAGTATAATGTT+TGG - chr2.4:9174371-9174390 None:intergenic 25.0%
! TAATAAATGTGAATCTTCCA+TGG + chr2.4:9175019-9175038 MS.gene31379:intron 25.0%
! TCCCTTTATATAGAAACTTT+AGG + chr2.4:9176771-9176790 MS.gene31379:intron 25.0%
! TTACTACTAATGATGATTGT+TGG - chr2.4:9176853-9176872 None:intergenic 25.0%
! TTAGAAAATGAAAACGTGTT+TGG + chr2.4:9174443-9174462 MS.gene31379:intron 25.0%
! TTGATTGATGCTTATATCAA+GGG - chr2.4:9177017-9177036 None:intergenic 25.0%
!! AAAGGGTATATTAGTAACTT+TGG + chr2.4:9176079-9176098 MS.gene31379:intron 25.0%
!! AACATATTTTCCTCTTACAA+AGG + chr2.4:9175130-9175149 MS.gene31379:intron 25.0%
!! AAGGGTATATTAGTAACTTT+GGG + chr2.4:9176080-9176099 MS.gene31379:intron 25.0%
!! CTCTAAATTTTACAAATTCG+TGG - chr2.4:9178449-9178468 None:intergenic 25.0%
!! GTTTTTAAATCCTTTGTAAG+AGG - chr2.4:9175143-9175162 None:intergenic 25.0%
!! TAAATTCAAGAGCTTTTGTT+TGG - chr2.4:9173988-9174007 None:intergenic 25.0%
!! TAAATTTTACAAATTCGTGG+TGG - chr2.4:9178446-9178465 None:intergenic 25.0%
!! TAATGGTAGTGAAATTATGT+TGG + chr2.4:9174711-9174730 MS.gene31379:intron 25.0%
!! TACTTTTGACAATTGAATTG+TGG - chr2.4:9174154-9174173 None:intergenic 25.0%
!! TGATTTAAGTTTACGTAGAA+GGG + chr2.4:9175693-9175712 MS.gene31379:intron 25.0%
!! TTAGTGAATGTTTTGTACTA+TGG - chr2.4:9178491-9178510 None:intergenic 25.0%
!! TTATTCTTTAAAAATGTCCC+CGG - chr2.4:9175002-9175021 None:intergenic 25.0%
!! TTCATTTTCATTGAAACTAG+AGG + chr2.4:9177461-9177480 MS.gene31379:intron 25.0%
!! TTGATTTAAGTTTACGTAGA+AGG + chr2.4:9175692-9175711 MS.gene31379:intron 25.0%
!! TTTTTGTTCTATTGAGAACA+AGG - chr2.4:9177078-9177097 None:intergenic 25.0%
!!! AACGTGTTTGGTATTTTTTT+AGG + chr2.4:9174455-9174474 MS.gene31379:intron 25.0%
!!! ACAAAAGTGTCACAAAATAT+TGG + chr2.4:9177374-9177393 MS.gene31379:intron 25.0%
!!! AGAAAAAGGCACTATATATT+AGG - chr2.4:9176690-9176709 None:intergenic 25.0%
!!! ATTCAATTGACGATTTTAGT+AGG - chr2.4:9175502-9175521 None:intergenic 25.0%
!!! CAAAAGTGTCACAAAATATT+GGG + chr2.4:9177375-9177394 MS.gene31379:intron 25.0%
!!! TTTGAAGGGATTATCTTTTT+TGG - chr2.4:9177274-9177293 None:intergenic 25.0%
!!! TTTTTTTAGGTTCAATGCAT+TGG + chr2.4:9174468-9174487 MS.gene31379:intron 25.0%
AAAAACTATTACCATGGCAA+TGG - chr2.4:9175061-9175080 None:intergenic 30.0%
AACAGAAGCTATGATAGAAA+TGG - chr2.4:9174256-9174275 None:intergenic 30.0%
AATTCCTAAAAAGAAGACCT+TGG - chr2.4:9178169-9178188 None:intergenic 30.0%
ACTAACCTGAAATGTTATTG+TGG - chr2.4:9174635-9174654 None:intergenic 30.0%
ACTACAACTATAAACATCCA+TGG - chr2.4:9175639-9175658 None:intergenic 30.0%
AGAAAATAGACATCACTGAA+AGG + chr2.4:9177485-9177504 MS.gene31379:intron 30.0%
AGAAGAAGTAAAAACAGAGT+CGG - chr2.4:9173953-9173972 None:intergenic 30.0%
AGATGGGATTATATTCAAGA+GGG + chr2.4:9178045-9178064 MS.gene31379:intron 30.0%
AGCATGTATGATTCATTCTT+GGG + chr2.4:9174567-9174586 MS.gene31379:CDS 30.0%
AGGGTATATTAGTAACTTTG+GGG + chr2.4:9176081-9176100 MS.gene31379:intron 30.0%
ATTAATCGAGTACGTATCAA+TGG - chr2.4:9175536-9175555 None:intergenic 30.0%
ATTGAATTGTGGATCTATGA+AGG - chr2.4:9174143-9174162 None:intergenic 30.0%
CTCAGTATCAGAAATATAGA+TGG + chr2.4:9174188-9174207 MS.gene31379:CDS 30.0%
CTTGATTGATGCTTATATCA+AGG - chr2.4:9177018-9177037 None:intergenic 30.0%
GAATTAAAGTGACTCAAAGT+AGG + chr2.4:9174314-9174333 MS.gene31379:intron 30.0%
GAGTAAATCTATCAGAATCA+CGG - chr2.4:9174343-9174362 None:intergenic 30.0%
GGGTGTAACTATGATTATAT+GGG - chr2.4:9175346-9175365 None:intergenic 30.0%
GGTGTAACTATGATTATATG+GGG - chr2.4:9175345-9175364 None:intergenic 30.0%
GGTGTTAAATATAGATCCTA+CGG - chr2.4:9177957-9177976 None:intergenic 30.0%
GTGAGCAATAAAGTATGAAA+CGG + chr2.4:9174952-9174971 MS.gene31379:intron 30.0%
TAGCATGTATGATTCATTCT+TGG + chr2.4:9174566-9174585 MS.gene31379:CDS 30.0%
TATCTCCACAATAACATTTC+AGG + chr2.4:9174627-9174646 MS.gene31379:CDS 30.0%
TCCTATGCAAAGAAAGAAAA+AGG - chr2.4:9176704-9176723 None:intergenic 30.0%
TGAGCAATAAAGTATGAAAC+GGG + chr2.4:9174953-9174972 MS.gene31379:intron 30.0%
TGGGTGTAACTATGATTATA+TGG - chr2.4:9175347-9175366 None:intergenic 30.0%
TGTTAGATTTAGTGTAGGTT+TGG + chr2.4:9174272-9174291 MS.gene31379:intron 30.0%
TTGAATTGTGGATCTATGAA+GGG - chr2.4:9174142-9174161 None:intergenic 30.0%
TTTGACATTTGAATGCAGAT+GGG + chr2.4:9178029-9178048 MS.gene31379:intron 30.0%
! AATAAAGTATGAAACGGGTT+TGG + chr2.4:9174958-9174977 MS.gene31379:intron 30.0%
! ACTTTGAGTCACTTTAATTC+TGG - chr2.4:9174314-9174333 None:intergenic 30.0%
! ATCCATTTTGTAGTTACTAG+CGG + chr2.4:9174864-9174883 MS.gene31379:intron 30.0%
! CGATGTTGTTAATTTTACCA+TGG + chr2.4:9175619-9175638 MS.gene31379:intron 30.0%
! GAAACACGTGTTTTCTTTTT+CGG - chr2.4:9176305-9176324 None:intergenic 30.0%
! TATTTCTGATACTGAGTATC+AGG - chr2.4:9174184-9174203 None:intergenic 30.0%
! TTAGATGAAGAGGACAATTT+TGG - chr2.4:9175396-9175415 None:intergenic 30.0%
! TTTCTTTTCCTGATCAAAAC+TGG + chr2.4:9175260-9175279 MS.gene31379:intron 30.0%
! TTTTCTCTCATTACTAGTAC+TGG - chr2.4:9174041-9174060 None:intergenic 30.0%
! TTTTGACATTTGAATGCAGA+TGG + chr2.4:9178028-9178047 MS.gene31379:intron 30.0%
!! AATGAAAATGAAAAGTGCCA+TGG - chr2.4:9177453-9177472 None:intergenic 30.0%
!! GATTTTCTAGCAAAGTATAG+GGG + chr2.4:9174490-9174509 MS.gene31379:CDS 30.0%
!! GGATTTTCTAGCAAAGTATA+GGG + chr2.4:9174489-9174508 MS.gene31379:CDS 30.0%
!! GTAGTTTACTGACTTTATGT+AGG - chr2.4:9177356-9177375 None:intergenic 30.0%
!! TAAATATGCCAGTTTTGATC+AGG - chr2.4:9175271-9175290 None:intergenic 30.0%
!! TGGATTTTCTAGCAAAGTAT+AGG + chr2.4:9174488-9174507 MS.gene31379:CDS 30.0%
!!! ATGTATTGGTTTTCAACACA+TGG + chr2.4:9177901-9177920 MS.gene31379:CDS 30.0%
!!! GTTTTTTTTTGTTGCGAAAC+CGG - chr2.4:9176660-9176679 None:intergenic 30.0%
AACACCAAATATAAGACGGA+TGG - chr2.4:9176355-9176374 None:intergenic 35.0%
AAGTTGGACTCAATAAAGAG+TGG + chr2.4:9177858-9177877 MS.gene31379:CDS 35.0%
ACACCAAGTAAACTGAGTTT+AGG - chr2.4:9175795-9175814 None:intergenic 35.0%
ACACCTAAACTCAGTTTACT+TGG + chr2.4:9175789-9175808 MS.gene31379:intron 35.0%
ACACGATTGATTGTGATTTG+TGG + chr2.4:9177690-9177709 MS.gene31379:intron 35.0%
ACTATCATTTACCATTGCCA+TGG + chr2.4:9175047-9175066 MS.gene31379:intron 35.0%
AGAAGAACATTCCAAGTATC+AGG - chr2.4:9178894-9178913 None:intergenic 35.0%
AGAGAAACACAACCATTCTT+TGG + chr2.4:9178684-9178703 MS.gene31379:CDS 35.0%
AGCTATGATAGAAATGGAGA+AGG - chr2.4:9174250-9174269 None:intergenic 35.0%
ATAACACTACTATACCCTTC+TGG - chr2.4:9178834-9178853 None:intergenic 35.0%
ATACACTACATTGTTGACAC+TGG - chr2.4:9177720-9177739 None:intergenic 35.0%
ATTGTACTTTCCCTCTTCTT+TGG + chr2.4:9175224-9175243 MS.gene31379:intron 35.0%
CAAATCACAATCAATCGTGT+TGG - chr2.4:9177690-9177709 None:intergenic 35.0%
CAATTAGGTGAAAGACAGAA+AGG - chr2.4:9174784-9174803 None:intergenic 35.0%
CAGATGGGATTATATTCAAG+AGG + chr2.4:9178044-9178063 MS.gene31379:intron 35.0%
CCACATTGTGTGATAAAAGA+AGG + chr2.4:9177398-9177417 MS.gene31379:intron 35.0%
CTACAAAGGTTTGACAACTT+GGG + chr2.4:9178107-9178126 MS.gene31379:CDS 35.0%
CTTGGTATAGAACAAGTGAA+AGG - chr2.4:9174217-9174236 None:intergenic 35.0%
CTTTCACTTGTTCTATACCA+AGG + chr2.4:9174215-9174234 MS.gene31379:CDS 35.0%
CTTTCTGTCTTTCACCTAAT+TGG + chr2.4:9174782-9174801 MS.gene31379:intron 35.0%
CTTTGTAGAACGCAATGAAA+CGG - chr2.4:9178095-9178114 None:intergenic 35.0%
GAATAGAGCTTAATCGCTTA+AGG - chr2.4:9176638-9176657 None:intergenic 35.0%
GCTTCTGTTAGATTTAGTGT+AGG + chr2.4:9174267-9174286 MS.gene31379:intron 35.0%
GGGTGTAACTTGGATTATTT+GGG + chr2.4:9176101-9176120 MS.gene31379:intron 35.0%
GGTGTAACTTGGATTATTTG+GGG + chr2.4:9176102-9176121 MS.gene31379:intron 35.0%
GTCACATCTAGAAAATACAC+TGG - chr2.4:9178780-9178799 None:intergenic 35.0%
GTGCAGTGAACATGAATAAA+GGG + chr2.4:9176062-9176081 MS.gene31379:intron 35.0%
GTGTGCGGAATAGAAATAAT+TGG - chr2.4:9177794-9177813 None:intergenic 35.0%
TAAGCGATTAAGCTCTATTC+CGG + chr2.4:9176638-9176657 MS.gene31379:intron 35.0%
TACATGCTAGTGAATTGAAC+TGG - chr2.4:9174555-9174574 None:intergenic 35.0%
TCTACAAAGGTTTGACAACT+TGG + chr2.4:9178106-9178125 MS.gene31379:CDS 35.0%
TCTCAAGTAGTGTAGTACTT+TGG + chr2.4:9177525-9177544 MS.gene31379:intron 35.0%
TGTGCAGTGAACATGAATAA+AGG + chr2.4:9176061-9176080 MS.gene31379:intron 35.0%
! CAAACCAAGGTCTTCTTTTT+AGG + chr2.4:9178162-9178181 MS.gene31379:CDS 35.0%
! CAATAAAGAGTGGTAATGCA+TGG + chr2.4:9177868-9177887 MS.gene31379:CDS 35.0%
! CCTTCTTTTATCACACAATG+TGG - chr2.4:9177401-9177420 None:intergenic 35.0%
! CTCATTACTAGTACTGGAAA+CGG - chr2.4:9174035-9174054 None:intergenic 35.0%
! GCCTTTTTCTTTCTTTGCAT+AGG + chr2.4:9176700-9176719 MS.gene31379:intron 35.0%
! GTGAATGTTTTGTACTATGG+TGG - chr2.4:9178488-9178507 None:intergenic 35.0%
! TATTGATTGCATATCCATGG+TGG - chr2.4:9175479-9175498 None:intergenic 35.0%
!! ACACATAGATTTTGGCATAC+TGG - chr2.4:9178422-9178441 None:intergenic 35.0%
!! AGGTATTGATTGCATATCCA+TGG - chr2.4:9175482-9175501 None:intergenic 35.0%
!! GGGAAAAGGTATTGACAATT+TGG - chr2.4:9176755-9176774 None:intergenic 35.0%
!!! GAGAAGGAATACCTTGTTTT+TGG - chr2.4:9174595-9174614 None:intergenic 35.0%
AACACGTGTTTCTCAGACTA+TGG + chr2.4:9176313-9176332 MS.gene31379:intron 40.0%
ACAAAACATGGGTTCACACA+AGG - chr2.4:9174827-9174846 None:intergenic 40.0%
ACAACTTACTCGTAATGCAC+AGG - chr2.4:9178195-9178214 None:intergenic 40.0%
ACGTGCAAAGAAGAAAAGGT+TGG - chr2.4:9176903-9176922 None:intergenic 40.0%
ACTTGCACAAAGTATGTGTG+AGG + chr2.4:9178358-9178377 MS.gene31379:intron 40.0%
AGAAAAGATGCACATCCAGA+AGG + chr2.4:9178816-9178835 MS.gene31379:CDS 40.0%
AGGAAAAGTCATGAAAGCAG+AGG - chr2.4:9177336-9177355 None:intergenic 40.0%
AGTAATGAGAGAAAACTCGC+TGG + chr2.4:9174046-9174065 MS.gene31379:CDS 40.0%
ATGGAGAGGAATGGATGTAT+TGG + chr2.4:9177887-9177906 MS.gene31379:CDS 40.0%
ATGTCTACGTTCCAAAGAAG+AGG - chr2.4:9175238-9175257 None:intergenic 40.0%
ATTCTTGGGTGCCAAAAACA+AGG + chr2.4:9174581-9174600 MS.gene31379:CDS 40.0%
CAAAGAACACATCACTTGCA+TGG - chr2.4:9174761-9174780 None:intergenic 40.0%
CAAGAAGAAGGATCATAGAG+AGG - chr2.4:9174118-9174137 None:intergenic 40.0%
CACCAATGACTACAATCTGT+TGG - chr2.4:9178858-9178877 None:intergenic 40.0%
CGTTTCATTGCGTTCTACAA+AGG + chr2.4:9178093-9178112 MS.gene31379:CDS 40.0%
GAAAAGATGCACATCCAGAA+GGG + chr2.4:9178817-9178836 MS.gene31379:CDS 40.0%
GATCATAGAGAGGATACGAA+GGG - chr2.4:9174108-9174127 None:intergenic 40.0%
GGAGAAGGAGATATATACCT+TGG - chr2.4:9174235-9174254 None:intergenic 40.0%
GGGGTGTAACTTGGATTATT+TGG + chr2.4:9176100-9176119 MS.gene31379:intron 40.0%
GGGTACTTCAAACCAAAGAA+TGG - chr2.4:9178699-9178718 None:intergenic 40.0%
TCCATGTTGGTGTGAAACAA+TGG + chr2.4:9174926-9174945 MS.gene31379:intron 40.0%
TCCATTGTTTCACACCAACA+TGG - chr2.4:9174930-9174949 None:intergenic 40.0%
TCTATGAAGGGACAAGAAGA+AGG - chr2.4:9174130-9174149 None:intergenic 40.0%
TCTATTCCGCACACCATATT+TGG + chr2.4:9177800-9177819 MS.gene31379:CDS 40.0%
TGCCAACAGATTGTAGTCAT+TGG + chr2.4:9178853-9178872 MS.gene31379:CDS 40.0%
TGGGATTATATTCAAGAGGG+TGG + chr2.4:9178048-9178067 MS.gene31379:CDS 40.0%
TGTAAATCCAGCTCAAACCA+AGG + chr2.4:9178149-9178168 MS.gene31379:CDS 40.0%
TGTCTACGTTCCAAAGAAGA+GGG - chr2.4:9175237-9175256 None:intergenic 40.0%
TTAAAAATGTCCCCGGCAAA+AGG - chr2.4:9174995-9175014 None:intergenic 40.0%
TTATATTCAAGAGGGTGGCA+AGG + chr2.4:9178053-9178072 MS.gene31379:CDS 40.0%
! AAAGTATGAAACGGGTTTGG+TGG + chr2.4:9174961-9174980 MS.gene31379:intron 40.0%
! AACTTGGATTATTTGGGGTG+TGG + chr2.4:9176107-9176126 MS.gene31379:intron 40.0%
! ACTTGGATTATTTGGGGTGT+GGG + chr2.4:9176108-9176127 MS.gene31379:intron 40.0%
! GTCATGTGGTCCATTTTGAA+GGG - chr2.4:9177288-9177307 None:intergenic 40.0%
! TAACTGGTTTCGTGGCAAAT+GGG + chr2.4:9174075-9174094 MS.gene31379:CDS 40.0%
! TCTCTCATGCATGATTTTGC+TGG - chr2.4:9178669-9178688 None:intergenic 40.0%
! TGGTTTTCAACACATGGCAT+TGG + chr2.4:9177907-9177926 MS.gene31379:CDS 40.0%
! TGTCATGTGGTCCATTTTGA+AGG - chr2.4:9177289-9177308 None:intergenic 40.0%
! TTTGACAACTTGGGCTAGAT+GGG + chr2.4:9178116-9178135 MS.gene31379:CDS 40.0%
!! AGTAACTTTGGGGTGTAACT+TGG + chr2.4:9176091-9176110 MS.gene31379:intron 40.0%
!! GATAGCACTTTGCTTTGAGA+AGG - chr2.4:9174611-9174630 None:intergenic 40.0%
!! TTTTCAACACATGGCATTGG+TGG + chr2.4:9177910-9177929 MS.gene31379:CDS 40.0%
!!! GTTTTCTTTTTCGGTCTAGC+TGG - chr2.4:9176296-9176315 None:intergenic 40.0%
AACTCGCTGGAAGATGTAAC+TGG + chr2.4:9174059-9174078 MS.gene31379:CDS 45.0%
ACCTTGACCGTGAGAACATT+GGG + chr2.4:9177826-9177845 MS.gene31379:CDS 45.0%
CAAAATGGACCACATGACAC+TGG + chr2.4:9177290-9177309 MS.gene31379:intron 45.0%
CGCCGCTAGTAACTACAAAA+TGG - chr2.4:9174869-9174888 None:intergenic 45.0%
CGCTCCATCCGTCTTATATT+TGG + chr2.4:9176348-9176367 MS.gene31379:intron 45.0%
CGGTCAAGGTCTACCAAATA+TGG - chr2.4:9177816-9177835 None:intergenic 45.0%
CTTCTTCATGCAGCTCTCTT+TGG + chr2.4:9178909-9178928 MS.gene31379:CDS 45.0%
GGATCATAGAGAGGATACGA+AGG - chr2.4:9174109-9174128 None:intergenic 45.0%
GGTAAATTAGGGGGAGAGAT+TGG + chr2.4:9178637-9178656 MS.gene31379:intron 45.0%
GGTCTACCAAATATGGTGTG+CGG - chr2.4:9177809-9177828 None:intergenic 45.0%
TCACTGAAAGGAGTTGAGTG+AGG + chr2.4:9177497-9177516 MS.gene31379:intron 45.0%
TGCATATCCATGGTGGTTTG+TGG - chr2.4:9175472-9175491 None:intergenic 45.0%
TGTGGGTGAAAAAGGGATGA+GGG - chr2.4:9175454-9175473 None:intergenic 45.0%
TTCACTATCACCCAAGCCAT+TGG - chr2.4:9178732-9178751 None:intergenic 45.0%
TTCCAAGTATCAGGAAGTCC+TGG - chr2.4:9178885-9178904 None:intergenic 45.0%
TTCCAGGACTTCCTGATACT+TGG + chr2.4:9178880-9178899 MS.gene31379:CDS 45.0%
TTGTGGGTGAAAAAGGGATG+AGG - chr2.4:9175455-9175474 None:intergenic 45.0%
! AAGAAGACCTTGGTTTGAGC+TGG - chr2.4:9178159-9178178 None:intergenic 45.0%
! AAGAGTGGTAATGCATGGAG+AGG + chr2.4:9177873-9177892 MS.gene31379:CDS 45.0%
! CAGAGGAGTGCATCTGAATT+AGG - chr2.4:9177319-9177338 None:intergenic 45.0%
! GCATATCCATGGTGGTTTGT+GGG - chr2.4:9175471-9175490 None:intergenic 45.0%
! GGAAGATGTAACTGGTTTCG+TGG + chr2.4:9174067-9174086 MS.gene31379:CDS 45.0%
! GGTAGTTGTTGCTGTTGTGT+TGG - chr2.4:9174014-9174033 None:intergenic 45.0%
! GGTGGTTTGTGGGTGAAAAA+GGG - chr2.4:9175461-9175480 None:intergenic 45.0%
! GTAACTGGTTTCGTGGCAAA+TGG + chr2.4:9174074-9174093 MS.gene31379:CDS 45.0%
! GTGGTGGCACACATAGATTT+TGG - chr2.4:9178430-9178449 None:intergenic 45.0%
! GTTACTAGCGGCGAATTTTG+TGG + chr2.4:9174876-9174895 MS.gene31379:intron 45.0%
! GTTTGACAACTTGGGCTAGA+TGG + chr2.4:9178115-9178134 MS.gene31379:CDS 45.0%
! TGGTAATGCATGGAGAGGAA+TGG + chr2.4:9177878-9177897 MS.gene31379:CDS 45.0%
! TGGTGGTTTGTGGGTGAAAA+AGG - chr2.4:9175462-9175481 None:intergenic 45.0%
! TGTAGTCATTGGTGTCTTCC+AGG + chr2.4:9178864-9178883 MS.gene31379:CDS 45.0%
! TTTTCACCCACAAACCACCA+TGG + chr2.4:9175462-9175481 MS.gene31379:intron 45.0%
!! CATTGGGCGTGTTCTAAAGT+TGG + chr2.4:9177842-9177861 MS.gene31379:CDS 45.0%
!! TTTGGTTTGAAGTACCCAGC+AGG + chr2.4:9178702-9178721 MS.gene31379:CDS 45.0%
!! ATTTAAAAAATACATTTATT+GGG + chr2.4:9175169-9175188 MS.gene31379:intron 5.0%
!! GTAAAAAATTTAATAAATTT+GGG - chr2.4:9175366-9175385 None:intergenic 5.0%
!! TATTTAAAAAATACATTTAT+TGG + chr2.4:9175168-9175187 MS.gene31379:intron 5.0%
!! TGTAAAAAATTTAATAAATT+TGG - chr2.4:9175367-9175386 None:intergenic 5.0%
!!! ATATTTAATTTATCTTTTTT+TGG + chr2.4:9175284-9175303 MS.gene31379:intron 5.0%
!!! TATTAATATTATTTTCAAAA+AGG - chr2.4:9177190-9177209 None:intergenic 5.0%
AATGGACCACATGACACTGG+TGG + chr2.4:9177293-9177312 MS.gene31379:intron 50.0%
ACGGTTGTGCATTTCCAGTG+TGG - chr2.4:9177938-9177957 None:intergenic 50.0%
AGCAGGAACACCAATGGCTT+GGG + chr2.4:9178719-9178738 MS.gene31379:CDS 50.0%
ATTAGGCCACCAGTGTCATG+TGG - chr2.4:9177302-9177321 None:intergenic 50.0%
CACTGGAAATGCACAACCGT+AGG + chr2.4:9177938-9177957 MS.gene31379:intron 50.0%
GAACACGCCCAATGTTCTCA+CGG - chr2.4:9177836-9177855 None:intergenic 50.0%
GACCTTGACCGTGAGAACAT+TGG + chr2.4:9177825-9177844 MS.gene31379:CDS 50.0%
GGGGACGTGCAAAGAAGAAA+AGG - chr2.4:9176907-9176926 None:intergenic 50.0%
! AAGCCATTGGTGTTCCTGCT+GGG - chr2.4:9178719-9178738 None:intergenic 50.0%
! GTGGAGGCTTTTCCTTTTGC+CGG + chr2.4:9174980-9174999 MS.gene31379:intron 50.0%
! TGGAGGCTTTTCCTTTTGCC+GGG + chr2.4:9174981-9175000 MS.gene31379:intron 50.0%
!! GTATGAAACGGGTTTGGTGG+AGG + chr2.4:9174964-9174983 MS.gene31379:intron 50.0%
CAGCAGGAACACCAATGGCT+TGG + chr2.4:9178718-9178737 MS.gene31379:CDS 55.0%
GCCCAATGTTCTCACGGTCA+AGG - chr2.4:9177830-9177849 None:intergenic 55.0%
GTACCCAGCAGGAACACCAA+TGG + chr2.4:9178713-9178732 MS.gene31379:CDS 55.0%
! CAAGCCATTGGTGTTCCTGC+TGG - chr2.4:9178720-9178739 None:intergenic 55.0%
! GGAGGCTTTTCCTTTTGCCG+GGG + chr2.4:9174982-9175001 MS.gene31379:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 9173935 9178935 9173935 ID=MS.gene31379
chr2.4 mRNA 9173935 9178935 9173935 ID=MS.gene31379.t1;Parent=MS.gene31379
chr2.4 exon 9173935 9174236 9173935 ID=MS.gene31379.t1.exon1;Parent=MS.gene31379.t1
chr2.4 CDS 9173935 9174236 9173935 ID=cds.MS.gene31379.t1;Parent=MS.gene31379.t1
chr2.4 exon 9174477 9174648 9174477 ID=MS.gene31379.t1.exon2;Parent=MS.gene31379.t1
chr2.4 CDS 9174477 9174648 9174477 ID=cds.MS.gene31379.t1;Parent=MS.gene31379.t1
chr2.4 exon 9177780 9177955 9177780 ID=MS.gene31379.t1.exon3;Parent=MS.gene31379.t1
chr2.4 CDS 9177780 9177955 9177780 ID=cds.MS.gene31379.t1;Parent=MS.gene31379.t1
chr2.4 exon 9178047 9178205 9178047 ID=MS.gene31379.t1.exon4;Parent=MS.gene31379.t1
chr2.4 CDS 9178047 9178205 9178047 ID=cds.MS.gene31379.t1;Parent=MS.gene31379.t1
chr2.4 exon 9178647 9178935 9178647 ID=MS.gene31379.t1.exon5;Parent=MS.gene31379.t1
chr2.4 CDS 9178647 9178935 9178647 ID=cds.MS.gene31379.t1;Parent=MS.gene31379.t1
Gene Sequence

>MS.gene31379

ATGGGTTTTCTGTTACCGACTCTGTTTTTACTTCTTCTGTTTTCTTCACACCAAACAAAAGCTCTTGAATTTAACTCCAACACAACAGCAACAACTACCGTTTCCAGTACTAGTAATGAGAGAAAACTCGCTGGAAGATGTAACTGGTTTCGTGGCAAATGGGTCTATGACCCTTCGTATCCTCTCTATGATCCTTCTTCTTGTCCCTTCATAGATCCACAATTCAATTGTCAAAAGTATGGCAGACCTGATACTCAGTATCAGAAATATAGATGGCAACCTTTCACTTGTTCTATACCAAGGTATATATCTCCTTCTCCATTTCTATCATAGCTTCTGTTAGATTTAGTGTAGGTTTGGTATCAGAGTGAATTCGCCAGAATTAAAGTGACTCAAAGTAGGTCACCGTGATTCTGATAGATTTACTCTGATACCAAACATTATACTAGCTACTTATATTGTATAACTAATTTTTATTTCTAATAGTTTTAAGTGTGATAAATGTAAGTTAGAAAATGAAAACGTGTTTGGTATTTTTTTAGGTTCAATGCATTGGATTTTCTAGCAAAGTATAGGGGAAAAAAGATTATGTTTGTTGGTGATTCTTTGAGCTTAAACCAGTTCAATTCACTAGCATGTATGATTCATTCTTGGGTGCCAAAAACAAGGTATTCCTTCTCAAAGCAAAGTGCTATCTCCACAATAACATTTCAGGTTAGTTTTTGTATTTCTATGTTTTTTTTTCTTTAATTTCGATTTTTTGATACTTTTGAATTTAATGGTAGTGAAATTATGTTGGTTATGTTTTAGTGTGTAATTGTGTCCATGCAAGTGATGTGTTCTTTGCCTTTCTGTCTTTCACCTAATTGGAAACTTTATTGAGTAATTGCCTTGTGTGAACCCATGTTTTGTTTTTCTTCAAATCTACGATCCATTTTGTAGTTACTAGCGGCGAATTTTGTGGTCAAATTATTATTTATTTTTATTATTTTCCATGTTGGTGTGAAACAATGGAGCGTGAGCAATAAAGTATGAAACGGGTTTGGTGGAGGCTTTTCCTTTTGCCGGGGACATTTTTAAAGAATAATAAATGTGAATCTTCCATGGTTATTACTATCATTTACCATTGCCATGGTAATAGTTTTTTCATAAAAAATGCCATGGTAATAATAGATAGTAATTCATGTTTTCTATTAACATATTTTCCTCTTACAAAGGATTTAAAAACAATAATATTTAAAAAATACATTTATTGGGGGGAAATATAAATATATTAACATTTTCATATGATTGTACTTTCCCTCTTCTTTGGAACGTAGACATTATTTCTTTTCCTGATCAAAACTGGCATATTTAATTTATCTTTTTTTGGTTGTTATGATTATTTAATGACTCTTTCTAGTTTCACCCCATATAATCATAGTTACACCCAAATTTATTAAATTTTTTACAATAATACCAAAATTGTCCTCTTCATCTAAATTTCTAAAAACCCTTCTTTTATTATCATTCTGAACCCTCATCCCTTTTTCACCCACAAACCACCATGGATATGCAATCAATACCTACTAAAATCGTCAATTGAATCAACTTCTCTTCCATTGATACGTACTCGATTAATACAAGTAAGTGAATTTCATAAATTTCTTCGAGTTTTTATTTATCGTTTGCTCTATGATTTATTCGATGTTGTTAATTTTACCATGGATGTTTATAGTTGTAGTTAATTTCACTTTTGTCTATCGACATCGATTTGTTTGATTTAAGTTTACGTAGAAGGGTAACATAATTTAAGTTTACATATAGGTCTCACGTGAATTTAGTTTACGTACATATTCTAAAAATGAATAACATACACCTAAACTCAGTTTACTTGGTGTATGTAAACTCAGTTTACATAAACTATGTAAACTCAGTTTACATGAACTAAAAACTCAGTCTTAACTTACTTATGTAAATTCAGTTTACATAGTGTATGTAAACTCAGTTTACGTAGTATATGTAAACTCAGTTTACTTAACTCATGTAAACTGAATTTACATAAACTACGTAAACTGAATTTACATACACTACGTAAACTGAGTTTACATACACTATGTAAACTGAATTTACGTATACATAATTAATGTGCAGTGAACATGAATAAAGGGTATATTAGTAACTTTGGGGTGTAACTTGGATTATTTGGGGTGTGGGCAGTATCTGTCTTATTTAATAGATGCTTGTTAGTATTCTTCAAACTTTTATCAAAGTATATAAATTTTAAAATATTTTTTTTTATACAAATATTCGATATATTTTAAAAGTTTAATTTCAATGTAATTTTTAATAAATATTTTAAAAGTTTAATTTCTTTAACCAGCTAGACCGAAAAAGAAAACACGTGTTTCTCAGACTATGGAATAGATGCATACGCTCCATCCGTCTTATATTTGGTGTTCTATTTAAGTTTGCATTAAGAATGTACCAAAAATGAAATTTGTATTGAAAGAATGATTAATAGTGTTGATTTAAATGATAAATATGTGTCATTTACTAAACATTCTTATTAATTATAATTAGTAAAATAATTATAGATAATTGAAATGCAGTAAATAAATTATATGTTAGTGGAAAAGAATAATACAATATTAGTATGGTAAAAAAAGATACAAACTAGTGGTTGAAGTCTTGAACGAGAGTTTCGTCATTTATATGTCACCTTAAGCGATTAAGCTCTATTCCGGTTTCGCAACAAAAAAAAACTATTGATCCTAATATATAGTGCCTTTTTCTTTCTTTGCATAGGAAATGCTCCAATAAAATTATATTCATGATCCAAATTGTCAATACCTTTTCCCTTTATATAGAAACTTTAGGAACAAATTTCTTAATTAGCAATAATTAGTGTTTTGGCATCTAAGTTTTCTAGCACTCCAACAATCATCATTAGTAGTAACTAAATTATGAACACAACGTTACTCAGCCAACCTTTTCTTCTTTGCACGTCCCCTTTTTAAACTGTAATGATCAATAATTTGATATCTAGTTTTTCTAACTTCAAGAAATATCTGATTGAATACATGCTTTATTATGTTTTCCCTTGATATAAGCATCAATCAAGCTATTCACATTAAAAAAATAGGAGTAAAAAATGTGTTCCTTGTTCTCAATAGAACAAAAAATAAAATTTGAAGTGGGCCATATGAATTAAATTGTAGTACTAACAGTAGTATATACTATATATTTATTCATTATAGGCTTGATGACATTCCTTTTTGAAAATAATATTAATACTGCATAAAATTAGTAATTTCTTCATTAATCTCTTGCATTTGCTGTCTTTCAATGTTTGTACCAAAAAAGATAATCCCTTCAAAATGGACCACATGACACTGGTGGCCTAATTCAGATGCACTCCTCTGCTTTCATGACTTTTCCTACATAAAGTCAGTAAACTACAAAAGTGTCACAAAATATTGGGCCACATTGTGTGATAAAAGAAGGAATTTTCCTTTCTTCTAGTAATTTATATTCCATGGCACTTTTCATTTTCATTGAAACTAGAGGCAGAAAATAGACATCACTGAAAGGAGTTGAGTGAGGAAATTTCTCAAGTAGTGTAGTACTTTGGTAGAAAGTTATTTAAATAGAAAATTAGATGAAAATTATGTACAATTATTATATGTAAGGAGTTTTTACACTAACAATCAATTTTAACCGTCAAATGATTTATAAAAGTTTGACTTTTATCATAATCACTTCTAAAACGACCAACACGATTGATTGTGATTTGTGGTGTGCCAGTGTCAACAATGTAGTGTATGAAAACTAAGCTTAATAATTATTATTTGAATTATTTTCAGGATTATGGTCTCCAATTATTTCTATTCCGCACACCATATTTGGTAGACCTTGACCGTGAGAACATTGGGCGTGTTCTAAAGTTGGACTCAATAAAGAGTGGTAATGCATGGAGAGGAATGGATGTATTGGTTTTCAACACATGGCATTGGTGGACCCACACTGGAAATGCACAACCGTAGGATCTATATTTAACACCATGATCATAATCAATTCTGTTATTAAGGAGGTGATTAATTAGTTGTTGTTTTTTTGACATTTGAATGCAGATGGGATTATATTCAAGAGGGTGGCAAGGTGTACAAAGACATGAACCGTTTCATTGCGTTCTACAAAGGTTTGACAACTTGGGCTAGATGGGTTAACATCAATGTAAATCCAGCTCAAACCAAGGTCTTCTTTTTAGGAATTTCTCCTGTGCATTACGAGTAAGTTGTTCTATCTACTTCAATTTAATCTATTATTTAGTGCGTGTTTGAATTCACAATGTTTTTTTACAGAATTACGGTGTACTATCGTGATTTTAAATTTCAGTAGAATCAGGGTCACTTTACTTTTGTTACACTTGCTATGACTTCAAACTTGCACAAAGTATGTGTGAGGTTTTACAAAAGAAATCATTAGAATGTTTGGTATCGCAGCCAGTATGCCAAAATCTATGTGTGCCACCACGAATTTGTAAAATTTAGAGTGTAGTTTTTGCAAAACCACCATAGTACAAAACATTCACTAAACTTGCTTATGTCAAAAGTAATTTTCGTAAAAGTGATAAAAAAAAGTTATTCTTTTTGTTACTAAATTGTGTGCCAATTACTAGACTAAATTTTATCAACTATATTTATAATGGTGTATGATATTTGGTAAATTAGGGGGAGAGATTGGAATCAACCAGCAAAATCATGCATGAGAGAAACACAACCATTCTTTGGTTTGAAGTACCCAGCAGGAACACCAATGGCTTGGGTGATAGTGAATAAAGTATTGAGCAGATTAAAGAAGCCAGTGTATTTTCTAGATGTGACAACATTGTCACAGTACAGAAAAGATGCACATCCAGAAGGGTATAGTAGTGTTATGCCAACAGATTGTAGTCATTGGTGTCTTCCAGGACTTCCTGATACTTGGAATGTTCTTCTTCATGCAGCTCTCTTTGGTTGA
Protein sequence

>MS.gene31379.t1

MGFLLPTLFLLLLFSSHQTKALEFNSNTTATTTVSSTSNERKLAGRCNWFRGKWVYDPSYPLYDPSSCPFIDPQFNCQKYGRPDTQYQKYRWQPFTCSIPRFNALDFLAKYRGKKIMFVGDSLSLNQFNSLACMIHSWVPKTRYSFSKQSAISTITFQDYGLQLFLFRTPYLVDLDRENIGRVLKLDSIKSGNAWRGMDVLVFNTWHWWTHTGNAQPWDYIQEGGKVYKDMNRFIAFYKGLTTWARWVNINVNPAQTKVFFLGISPVHYEGRDWNQPAKSCMRETQPFFGLKYPAGTPMAWVIVNKVLSRLKKPVYFLDVTTLSQYRKDAHPEGYSSVMPTDCSHWCLPGLPDTWNVLLHAALFG