AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043504

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043504.t1 MTR_2g091175 97.554 368 6 1 1 365 1 368 0.0 750
MS.gene043504.t1 MTR_4g079700 75.278 360 84 1 4 363 9 363 0.0 585
MS.gene043504.t1 MTR_2g015720 59.563 366 137 5 1 363 1 358 2.36e-159 451
MS.gene043504.t1 MTR_4g087730 56.164 365 157 2 1 364 1 363 5.89e-153 435
MS.gene043504.t1 MTR_4g088030 56.011 366 144 4 1 364 1 351 8.34e-149 424
MS.gene043504.t1 MTR_3g049330 54.829 321 142 2 47 364 31 351 2.02e-133 385
MS.gene043504.t1 MTR_2g073510 55.799 319 137 3 46 360 32 350 2.90e-131 380
MS.gene043504.t1 MTR_3g021060 52.905 327 153 1 38 363 37 363 3.62e-130 377
MS.gene043504.t1 MTR_3g035830 50.448 335 165 1 30 363 14 348 2.79e-127 369
MS.gene043504.t1 MTR_3g021090 52.548 314 148 1 47 359 31 344 5.56e-124 363
MS.gene043504.t1 MTR_5g034810 51.796 334 155 4 35 364 22 353 6.71e-123 358
MS.gene043504.t1 MTR_3g464570 48.358 335 156 4 47 364 43 377 1.59e-115 340
MS.gene043504.t1 MTR_5g071350 44.504 373 166 5 17 364 44 400 2.19e-115 341
MS.gene043504.t1 MTR_2g073500 45.289 329 158 8 47 364 29 346 4.75e-101 302
MS.gene043504.t1 MTR_1g050448 42.941 340 170 8 39 363 45 375 1.42e-95 290
MS.gene043504.t1 MTR_1g111010 45.897 329 157 10 44 365 29 343 6.28e-93 282
MS.gene043504.t1 MTR_1g112210 38.040 347 194 6 31 363 43 382 1.80e-91 279
MS.gene043504.t1 MTR_1g112210 39.143 350 173 9 31 363 43 369 7.52e-91 277
MS.gene043504.t1 MTR_0136s0010 38.400 375 204 6 15 364 138 510 3.63e-90 280
MS.gene043504.t1 MTR_7g093450 40.233 343 180 6 46 364 247 588 2.09e-89 281
MS.gene043504.t1 MTR_3g464570 47.407 270 125 4 112 364 4 273 5.16e-84 256
MS.gene043504.t1 MTR_4g126950 37.318 343 186 6 46 363 108 446 5.21e-84 262
MS.gene043504.t1 MTR_5g033800 35.342 365 212 6 23 364 121 484 2.57e-83 262
MS.gene043504.t1 MTR_4g121560 38.338 373 202 9 18 364 84 454 1.86e-81 256
MS.gene043504.t1 MTR_3g070900 39.017 346 184 9 47 365 106 451 2.19e-81 256
MS.gene043504.t1 MTR_7g109550 39.759 332 176 8 47 359 137 463 3.37e-80 253
MS.gene043504.t1 MTR_8g069790 37.356 348 188 6 43 364 174 517 5.88e-80 254
MS.gene043504.t1 MTR_5g082880 38.109 349 186 10 47 365 165 513 1.42e-78 250
MS.gene043504.t1 MTR_5g059400 36.827 353 193 8 39 364 167 516 1.79e-78 250
MS.gene043504.t1 MTR_1g116130 36.827 353 193 8 39 364 167 516 1.79e-78 250
MS.gene043504.t1 MTR_3g071650 34.085 399 227 10 2 364 33 431 1.05e-75 240
MS.gene043504.t1 MTR_5g083260 37.069 348 194 7 42 364 78 425 4.57e-75 238
MS.gene043504.t1 MTR_4g016940 34.000 400 219 11 9 363 28 427 1.24e-74 237
MS.gene043504.t1 MTR_1g058380 37.026 343 189 8 47 364 55 395 8.50e-73 232
MS.gene043504.t1 MTR_8g463790 35.613 351 196 9 40 364 64 410 6.03e-71 228
MS.gene043504.t1 MTR_2g022560 37.059 340 188 7 47 361 97 435 1.10e-68 223
MS.gene043504.t1 MTR_7g094750 36.812 345 189 8 47 364 91 433 3.81e-68 221
MS.gene043504.t1 MTR_4g014430 35.632 348 198 9 36 359 97 442 4.73e-68 221
MS.gene043504.t1 MTR_4g014440 32.687 361 209 9 33 364 53 408 6.64e-67 217
MS.gene043504.t1 MTR_7g070060 31.969 391 230 10 4 365 28 411 1.89e-66 216
MS.gene043504.t1 MTR_7g068920 35.838 346 195 9 44 363 77 421 4.88e-66 215
MS.gene043504.t1 MTR_3g090120 36.202 337 191 7 47 364 129 460 4.26e-62 206
MS.gene043504.t1 MTR_5g033800 34.749 259 157 4 23 269 121 379 1.21e-54 184
MS.gene043504.t1 MTR_8g093530 30.029 343 220 6 37 364 80 417 3.49e-53 182
MS.gene043504.t1 MTR_5g069320 33.224 304 143 9 74 356 122 386 1.69e-45 160
MS.gene043504.t1 MTR_7g094750 35.294 238 140 3 47 270 91 328 5.15e-45 157
MS.gene043504.t1 MTR_7g070060 30.545 275 174 5 4 268 28 295 9.11e-45 156
MS.gene043504.t1 MTR_7g070060 30.195 308 186 9 87 365 2 309 7.99e-42 149
MS.gene043504.t1 MTR_6g016880 29.008 393 231 11 4 363 42 419 3.28e-39 144
MS.gene043504.t1 MTR_1g076690 28.792 389 230 13 12 359 184 566 4.03e-38 144
MS.gene043504.t1 MTR_5g035090 30.049 406 224 19 4 359 28 423 5.82e-38 141
MS.gene043504.t1 MTR_5g035080 30.102 392 219 17 9 359 39 416 8.31e-38 140
MS.gene043504.t1 MTR_6g061710 27.632 380 233 11 16 359 137 510 3.47e-36 138
MS.gene043504.t1 MTR_7g019120 28.161 348 207 9 46 359 78 416 1.30e-34 132
MS.gene043504.t1 MTR_6g016875 28.244 393 235 14 3 359 23 404 1.68e-34 132
MS.gene043504.t1 MTR_6g016240 30.725 345 204 12 47 359 71 412 3.20e-34 131
MS.gene043504.t1 MTR_8g093870 31.389 360 206 17 35 363 123 472 3.28e-34 132
MS.gene043504.t1 MTR_5g035150 30.636 346 204 11 47 359 85 427 7.69e-34 130
MS.gene043504.t1 MTR_8g027900 27.174 368 220 10 34 363 198 555 7.40e-32 126
MS.gene043504.t1 MTR_2g016060 28.940 349 203 13 48 359 56 396 7.44e-32 124
MS.gene043504.t1 MTR_5g035070 29.480 346 208 12 47 359 87 429 1.22e-31 124
MS.gene043504.t1 MTR_7g033230 28.532 361 205 15 40 359 257 605 8.71e-31 123
MS.gene043504.t1 MTR_7g033230 28.532 361 205 15 40 359 327 675 1.15e-30 123
MS.gene043504.t1 MTR_4g092830 27.666 347 211 13 47 359 48 388 8.26e-30 119
MS.gene043504.t1 MTR_8g104970 27.397 365 218 13 33 359 106 461 3.21e-29 118
MS.gene043504.t1 MTR_3g021150 44.737 114 62 1 231 343 1 114 4.64e-29 109
MS.gene043504.t1 MTR_4g125060 29.395 347 208 15 47 363 132 471 5.31e-29 117
MS.gene043504.t1 MTR_4g125060 29.395 347 208 15 47 363 200 539 6.09e-29 117
MS.gene043504.t1 MTR_4g125060 29.395 347 208 15 47 363 171 510 7.56e-29 117
MS.gene043504.t1 MTR_6g007777 28.788 396 218 18 14 363 80 457 2.08e-28 115
MS.gene043504.t1 MTR_3g109840 27.089 395 240 14 3 359 27 411 5.93e-28 114
MS.gene043504.t1 MTR_8g099300 27.066 351 215 13 44 359 71 415 1.48e-27 112
MS.gene043504.t1 MTR_7g019140 44.444 90 50 0 46 135 78 167 4.89e-22 92.0
MS.gene043504.t1 MTR_5g035120 38.095 105 65 0 37 141 65 169 1.84e-18 82.4
MS.gene043504.t1 MTR_2g022580 32.773 119 66 3 107 211 2 120 2.56e-12 63.9
MS.gene043504.t1 MTR_3g021080 31.683 101 49 2 265 364 99 180 5.29e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043504.t1 AT2G42570 66.279 344 111 3 26 364 22 365 3.96e-180 504
MS.gene043504.t1 AT2G31110 62.928 321 118 1 44 363 42 362 6.60e-166 468
MS.gene043504.t1 AT1G29050 59.882 339 134 2 27 363 40 378 1.51e-157 447
MS.gene043504.t1 AT2G34070 59.692 325 129 2 41 363 59 383 2.62e-150 429
MS.gene043504.t1 AT3G14850 55.108 323 141 2 42 360 29 351 4.32e-131 379
MS.gene043504.t1 AT1G78710 50.153 327 157 4 42 364 33 357 2.52e-121 355
MS.gene043504.t1 AT2G30900 50.000 324 156 4 47 364 43 366 1.68e-119 350
MS.gene043504.t1 AT2G34070 60.643 249 96 2 117 363 1 249 1.94e-111 325
MS.gene043504.t1 AT5G58600 47.619 336 158 5 47 364 66 401 1.21e-110 329
MS.gene043504.t1 AT2G31110 61.927 218 83 0 44 261 42 259 7.85e-108 317
MS.gene043504.t1 AT2G30010 45.322 342 163 5 47 364 56 397 3.25e-105 315
MS.gene043504.t1 AT2G30010 45.322 342 163 5 47 364 99 440 3.32e-105 317
MS.gene043504.t1 AT2G31110 63.768 207 74 1 158 363 8 214 4.15e-104 305
MS.gene043504.t1 AT3G14850 55.242 248 107 2 117 360 1 248 2.35e-95 285
MS.gene043504.t1 AT3G54260 42.515 334 167 6 47 364 51 375 9.47e-92 280
MS.gene043504.t1 AT5G06700 42.274 343 171 7 47 364 255 595 4.39e-91 285
MS.gene043504.t1 AT3G12060 39.678 373 197 7 19 364 165 536 3.82e-89 279
MS.gene043504.t1 AT1G78710 41.896 327 131 5 42 364 26 297 1.44e-87 266
MS.gene043504.t1 AT2G30900 50.424 236 109 3 47 280 43 272 1.18e-84 258
MS.gene043504.t1 AT3G62390 38.544 371 201 9 19 364 104 472 1.70e-82 259
MS.gene043504.t1 AT5G58600 51.786 224 106 2 47 268 66 289 6.06e-82 252
MS.gene043504.t1 AT5G20590 38.571 350 184 9 41 364 136 480 7.21e-82 258
MS.gene043504.t1 AT5G49340 37.960 353 190 8 39 364 92 442 1.23e-80 254
MS.gene043504.t1 AT1G60790 38.211 369 194 10 26 364 169 533 4.05e-80 255
MS.gene043504.t1 AT3G55990 37.464 347 190 9 46 365 139 485 3.52e-77 246
MS.gene043504.t1 AT2G40150 35.977 353 193 8 46 365 70 422 8.55e-76 240
MS.gene043504.t1 AT3G11030 38.000 350 179 10 47 364 106 449 9.19e-75 238
MS.gene043504.t1 AT2G40160 35.817 349 192 9 47 364 77 424 3.37e-74 237
MS.gene043504.t1 AT2G40160 35.569 343 191 8 47 359 77 419 5.35e-74 236
MS.gene043504.t1 AT1G73140 36.544 353 194 8 38 364 58 406 4.61e-73 233
MS.gene043504.t1 AT5G19160 36.388 371 193 10 24 361 82 442 5.27e-73 234
MS.gene043504.t1 AT5G01360 35.756 344 195 7 46 363 35 378 4.93e-72 229
MS.gene043504.t1 AT5G01360 35.756 344 195 7 46 363 91 434 2.26e-71 229
MS.gene043504.t1 AT5G01620 34.402 343 198 8 42 359 113 453 9.93e-71 228
MS.gene043504.t1 AT5G01620 34.402 343 198 8 42 359 105 445 1.14e-70 228
MS.gene043504.t1 AT5G01620 34.402 343 198 8 42 359 105 445 1.14e-70 228
MS.gene043504.t1 AT2G40320 35.632 348 199 8 42 364 76 423 8.05e-70 225
MS.gene043504.t1 AT3G06080 36.550 342 189 7 47 361 103 443 1.21e-69 226
MS.gene043504.t1 AT5G06230 35.574 357 201 7 36 364 10 365 4.23e-68 219
MS.gene043504.t1 AT5G06230 35.574 357 201 7 36 364 51 406 1.17e-67 219
MS.gene043504.t1 AT3G11570 37.538 325 180 7 63 365 98 421 5.39e-67 218
MS.gene043504.t1 AT2G38320 33.333 351 204 9 44 364 55 405 3.79e-64 210
MS.gene043504.t1 AT1G48880 33.699 365 195 8 21 364 102 440 1.14e-61 204
MS.gene043504.t1 AT3G62390 37.918 269 149 6 19 269 104 372 2.77e-57 192
MS.gene043504.t1 AT5G20590 39.431 246 128 7 41 269 136 377 1.38e-55 186
MS.gene043504.t1 AT5G19160 36.923 260 144 4 24 269 82 335 1.00e-53 181
MS.gene043504.t1 AT2G38320 31.056 322 194 7 71 364 1 322 4.54e-53 179
MS.gene043504.t1 AT2G40160 35.470 234 139 4 47 268 77 310 3.09e-50 171
MS.gene043504.t1 AT3G06080 37.131 237 135 3 47 269 103 339 4.24e-49 169
MS.gene043504.t1 AT5G06230 35.827 254 143 4 36 269 51 304 4.28e-46 160
MS.gene043504.t1 AT2G14530 29.306 389 239 12 7 363 22 406 3.35e-40 147
MS.gene043504.t1 AT5G51640 28.571 378 222 11 23 359 114 484 4.81e-40 148
MS.gene043504.t1 AT1G70230 29.513 349 198 11 47 363 79 411 6.23e-40 146
MS.gene043504.t1 AT4G25360 27.990 393 219 12 18 359 141 520 7.86e-37 140
MS.gene043504.t1 AT4G25360 27.990 393 219 12 18 359 141 520 7.86e-37 140
MS.gene043504.t1 AT5G64020 29.494 356 208 12 47 363 57 408 5.70e-35 132
MS.gene043504.t1 AT5G64470 26.744 344 223 10 47 364 55 395 1.53e-34 131
MS.gene043504.t1 AT1G01430 29.301 372 215 12 32 365 88 449 1.24e-33 130
MS.gene043504.t1 AT5G64470 26.857 350 221 11 47 364 55 401 3.67e-33 127
MS.gene043504.t1 AT5G15890 28.065 367 219 12 25 359 165 518 1.48e-32 128
MS.gene043504.t1 AT5G15900 26.934 349 217 11 47 363 67 409 3.93e-32 125
MS.gene043504.t1 AT2G37720 30.939 362 183 14 47 363 143 482 4.67e-32 126
MS.gene043504.t1 AT5G15900 26.934 349 217 11 47 363 86 428 4.77e-32 125
MS.gene043504.t1 AT5G20680 28.226 372 220 14 23 363 102 457 1.95e-31 124
MS.gene043504.t1 AT3G28150 30.966 352 199 16 46 363 69 410 2.89e-31 122
MS.gene043504.t1 AT3G02440 26.513 347 212 12 47 359 127 464 4.34e-31 123
MS.gene043504.t1 AT3G02440 26.513 347 212 12 47 359 135 472 4.66e-31 123
MS.gene043504.t1 AT5G20680 28.226 372 220 14 23 363 178 533 5.26e-31 123
MS.gene043504.t1 AT5G20680 29.023 348 208 13 47 363 212 551 7.01e-31 123
MS.gene043504.t1 AT5G20680 29.023 348 208 13 47 363 212 551 7.01e-31 123
MS.gene043504.t1 AT5G20680 29.023 348 208 13 47 363 212 551 7.01e-31 123
MS.gene043504.t1 AT5G20680 29.023 348 208 13 47 363 212 551 7.01e-31 123
MS.gene043504.t1 AT4G11090 26.463 393 230 16 21 365 47 428 7.48e-29 116
MS.gene043504.t1 AT4G01080 27.901 362 214 12 33 359 77 426 2.91e-28 115
MS.gene043504.t1 AT4G23790 25.683 366 213 14 46 365 78 430 5.26e-23 99.8
MS.gene043504.t1 AT5G64470 26.378 254 166 8 47 282 55 305 5.49e-21 92.8
MS.gene043504.t1 AT5G64470 26.378 254 166 8 47 282 55 305 7.25e-21 92.4

Find 75 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCATGCAGCTCTCTT+TGG 0.210145 2.2:+8252183 MS.gene043504:CDS
TCTATTCCGCACACCATATT+TGG 0.219542 2.2:+8251075 MS.gene043504:CDS
TATCTCCACAATAACATTTC+AGG 0.240854 2.2:+8248688 MS.gene043504:CDS
AGAGAAACACAACCATTCTT+TGG 0.302957 2.2:+8251958 MS.gene043504:CDS
CGGTCAAGGTCTACCAAATA+TGG 0.307611 2.2:-8251088 None:intergenic
GTTTGACAACTTGGGCTAGA+TGG 0.309964 2.2:+8251390 MS.gene043504:CDS
TAGCATGTATGATTCATTCT+TGG 0.316438 2.2:+8248627 MS.gene043504:CDS
TGTAGTCATTGGTGTCTTCC+AGG 0.323483 2.2:+8252138 MS.gene043504:CDS
GTGTGCGGAATAGAAATAAT+TGG 0.347634 2.2:-8251066 None:intergenic
CAAGCCATTGGTGTTCCTGC+TGG 0.351195 2.2:-8251991 None:intergenic
AGAAGAACATTCCAAGTATC+AGG 0.357411 2.2:-8252165 None:intergenic
TCTACAAAGGTTTGACAACT+TGG 0.371927 2.2:+8251381 MS.gene043504:CDS
TTCCAGGACTTCCTGATACT+TGG 0.372897 2.2:+8252154 MS.gene043504:CDS
CAATTCAATTGTCAAAAGTA+TGG 0.391925 2.2:+8248215 MS.gene043504:CDS
TATTTCTGATACTGAGTATC+AGG 0.397938 2.2:-8248242 None:intergenic
ACCTTGACCGTGAGAACATT+GGG 0.399023 2.2:+8251101 MS.gene043504:CDS
AGCATGTATGATTCATTCTT+GGG 0.407416 2.2:+8248628 MS.gene043504:CDS
CTCATTACTACTACTGGAAA+TGG 0.409028 2.2:-8248093 None:intergenic
AAGAAGACCTTGGTTTGAGC+TGG 0.426020 2.2:-8251431 None:intergenic
GTAACTGGTTTCGTGGCAAA+TGG 0.428187 2.2:+8248135 MS.gene043504:CDS
CTCAGTATCAGAAATATAGA+TGG 0.444563 2.2:+8248249 MS.gene043504:CDS
TGCCAACAGATTGTAGTCAT+TGG 0.444942 2.2:+8252127 MS.gene043504:CDS
AGCAGGAACACCAATGGCTT+GGG 0.468530 2.2:+8251993 MS.gene043504:CDS
TACATGCTAGTGAATTGAAC+TGG 0.471439 2.2:-8248613 None:intergenic
AACTTGCTGGAAGATGTAAC+TGG 0.472478 2.2:+8248120 MS.gene043504:CDS
TTCACTATTACCCAAGCCAT+TGG 0.475251 2.2:-8252003 None:intergenic
TTTGACAACTTGGGCTAGAT+GGG 0.479154 2.2:+8251391 MS.gene043504:CDS
ATGGAGAGGGATGGATGTAT+TGG 0.479481 2.2:+8251162 MS.gene043504:CDS
ATAACACTACTATACCCTTC+TGG 0.481855 2.2:-8252105 None:intergenic
CATTGGGCGTGTTCTAAAGT+TGG 0.484902 2.2:+8251117 MS.gene043504:CDS
ATTGAATTGTGGATCTATGA+AGG 0.486450 2.2:-8248201 None:intergenic
TTCCAAGTATCAGGAAGTCC+TGG 0.489986 2.2:-8252156 None:intergenic
AGTAATGAGAGAAAACTTGC+TGG 0.493842 2.2:+8248107 MS.gene043504:CDS
GGTAGTTGTTGCTGTTGTGT+TGG 0.501527 2.2:-8248072 None:intergenic
AAGCCATTGGTGTTCCTGCT+GGG 0.502968 2.2:-8251990 None:intergenic
CAGCAGGAACACCAATGGCT+TGG 0.504711 2.2:+8251992 MS.gene043504:CDS
CGGTTGTGCATTTCCAGTGT+GGG 0.509875 2.2:-8251209 None:intergenic
CTTTGTAGAACGCAATGAAA+CGG 0.518428 2.2:-8251367 None:intergenic
TAACTGGTTTCGTGGCAAAT+GGG 0.526407 2.2:+8248136 MS.gene043504:CDS
CGTTTCATTGCGTTCTACAA+AGG 0.529142 2.2:+8251368 MS.gene043504:CDS
GATAGCACTTTGCTTTGAGA+AGG 0.530854 2.2:-8248669 None:intergenic
CTACAAAGGTTTGACAACTT+GGG 0.543154 2.2:+8251382 MS.gene043504:CDS
TGGCATTGGTGGACCCACAC+TGG 0.546946 2.2:+8251196 MS.gene043504:CDS
GACCTTGACCGTGAGAACAT+TGG 0.550954 2.2:+8251100 MS.gene043504:CDS
TCTATGAAGGGACAAGAAGA+AGG 0.551169 2.2:-8248188 None:intergenic
GAAAAGATGCACATCCAGAA+GGG 0.559145 2.2:+8252091 MS.gene043504:CDS
AGAGTGGTAATGCATGGAGA+GGG 0.562652 2.2:+8251149 MS.gene043504:CDS
TTTGGTTTGAAGTACCCAGC+AGG 0.566621 2.2:+8251976 MS.gene043504:CDS
GGATCATAGAGAGGATACGA+AGG 0.570131 2.2:-8248167 None:intergenic
GGGTACTTCAAACCAAAGAA+TGG 0.571214 2.2:-8251970 None:intergenic
CTTTCACTTGTTCTATACCA+AGG 0.575010 2.2:+8248276 MS.gene043504:CDS
CAGATGGGATTATATTCAAG+AGG 0.575480 2.2:+8251319 MS.gene043504:intron
ACTAACCTGAAATGTTATTG+TGG 0.576103 2.2:-8248693 None:intergenic
CAATAAAGAGTGGTAATGCA+TGG 0.581612 2.2:+8251143 MS.gene043504:CDS
ACGGTTGTGCATTTCCAGTG+TGG 0.583332 2.2:-8251210 None:intergenic
AGAAAAGATGCACATCCAGA+AGG 0.583703 2.2:+8252090 MS.gene043504:CDS
AAGAGTGGTAATGCATGGAG+AGG 0.584992 2.2:+8251148 MS.gene043504:CDS
CACCAATGACTACAATCTGT+TGG 0.588126 2.2:-8252129 None:intergenic
GCCCAATGTTCTCACGGTCA+AGG 0.590015 2.2:-8251102 None:intergenic
ACAACTTACTCGTAATGCAC+AGG 0.597709 2.2:-8251467 None:intergenic
TTGAATTGTGGATCTATGAA+GGG 0.607685 2.2:-8248200 None:intergenic
TGTAAATCCAGCTCAAACCA+AGG 0.609530 2.2:+8251424 MS.gene043504:CDS
TGGTAATGCATGGAGAGGGA+TGG 0.611599 2.2:+8251153 MS.gene043504:CDS
GGAAGATGTAACTGGTTTCG+TGG 0.617818 2.2:+8248128 MS.gene043504:CDS
CTTGGTATAGAACAAGTGAA+AGG 0.623660 2.2:-8248275 None:intergenic
AGATGGGATTATATTCAAGA+GGG 0.628534 2.2:+8251320 MS.gene043504:intron
GAACACGCCCAATGTTCTCA+CGG 0.633134 2.2:-8251108 None:intergenic
TTATATTCAAGAGGGTGGCA+AGG 0.635985 2.2:+8251328 MS.gene043504:CDS
TGGGATTATATTCAAGAGGG+TGG 0.638991 2.2:+8251323 MS.gene043504:CDS
CAAGAAGAAGGATCATAGAG+AGG 0.640153 2.2:-8248176 None:intergenic
GTACCCAGCAGGAACACCAA+TGG 0.661011 2.2:+8251987 MS.gene043504:CDS
GGTCTACCAAATATGGTGTG+CGG 0.672109 2.2:-8251081 None:intergenic
GATCATAGAGAGGATACGAA+GGG 0.672935 2.2:-8248166 None:intergenic
GTCACATCTAGAAAATACAC+TGG 0.675733 2.2:-8252051 None:intergenic
AAGTTGGACTCAATAAAGAG+TGG 0.679146 2.2:+8251133 MS.gene043504:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAAATACATTTATTG+GGG + chr2.2:8249236-8249255 MS.gene043504:intron 10.0%
!! TTTATCAACTATATTTATAA+TGG + chr2.2:8251876-8251895 MS.gene043504:intron 10.0%
!!! CTATATATTTTATTCATTAT+AGG + chr2.2:8250396-8250415 MS.gene043504:intron 10.0%
!!! TTATTATTTGAATTATTTTC+AGG + chr2.2:8251033-8251052 MS.gene043504:intron 10.0%
!! AATATAAATTACTAGAAGAA+AGG - chr2.2:8250674-8250693 None:intergenic 15.0%
!! ACAAAAAATAAAATTTGAAG+TGG + chr2.2:8250334-8250353 MS.gene043504:intron 15.0%
!! AGAATAATACAATATTAGTA+TGG + chr2.2:8249788-8249807 MS.gene043504:intron 15.0%
!! ATGTACAATTATTATATGTA+AGG + chr2.2:8250827-8250846 MS.gene043504:intron 15.0%
!! GTAAATAAATTATATGTTAG+TGG + chr2.2:8249762-8249781 MS.gene043504:intron 15.0%
!! TACTAAAGTTTCTATATAAA+GGG - chr2.2:8250012-8250031 None:intergenic 15.0%
!! TTAAAAAATACATTTATTGG+GGG + chr2.2:8249237-8249256 MS.gene043504:intron 15.0%
!! TTACTAAAGTTTCTATATAA+AGG - chr2.2:8250013-8250032 None:intergenic 15.0%
!!! AATGTTTTTTTACAGAATTA+CGG + chr2.2:8251538-8251557 MS.gene043504:intron 15.0%
!!! ATTTTTATTATTTTCCATGT+TGG + chr2.2:8248979-8248998 MS.gene043504:intron 15.0%
!!! CAAAAAATAAAATTTGAAGT+GGG + chr2.2:8250335-8250354 MS.gene043504:intron 15.0%
!!! GATCATGAATATAATTTTAT+TGG - chr2.2:8249970-8249989 None:intergenic 15.0%
!!! TTCAATACAAATTTCATTTT+TGG - chr2.2:8249641-8249660 None:intergenic 15.0%
!!! TTTGATACTTTTGAATTTAA+TGG + chr2.2:8248755-8248774 MS.gene043504:intron 15.0%
!! AAAGAAATCATTAGAATGTT+TGG + chr2.2:8251661-8251680 MS.gene043504:intron 20.0%
!! AAATAGAACCAAATATAAGA+CGG - chr2.2:8249598-8249617 None:intergenic 20.0%
!! ATAAAACTTAGTCTAGTAAT+TGG - chr2.2:8251861-8251880 None:intergenic 20.0%
!! ATTACTATCTATAATTACCA+TGG - chr2.2:8249162-8249181 None:intergenic 20.0%
!! ATTTGAAGAAAAACAAAACA+TGG - chr2.2:8248900-8248919 None:intergenic 20.0%
!! CATAATCAATTCTGTTATTA+AGG + chr2.2:8251257-8251276 MS.gene043504:intron 20.0%
!! GCTATTCACATTAAAAAAAT+AGG + chr2.2:8250280-8250299 MS.gene043504:intron 20.0%
!! GTACTACAATTTAATTCATA+TGG - chr2.2:8250361-8250380 None:intergenic 20.0%
!! GTATGATATTTGGTAAATTA+GGG + chr2.2:8251900-8251919 MS.gene043504:intron 20.0%
!! TAAAAAAAGATACAAACTAG+TGG + chr2.2:8249811-8249830 MS.gene043504:intron 20.0%
!! TAAAAAATACATTTATTGGG+GGG + chr2.2:8249238-8249257 MS.gene043504:intron 20.0%
!! TATGATATTTGGTAAATTAG+GGG + chr2.2:8251901-8251920 MS.gene043504:intron 20.0%
!! TGATCATTACAGTTTAAAAA+GGG - chr2.2:8250164-8250183 None:intergenic 20.0%
!! TGTATGATATTTGGTAAATT+AGG + chr2.2:8251899-8251918 MS.gene043504:intron 20.0%
!! TTAGAAAATGAAAATGTGTT+TGG + chr2.2:8248504-8248523 MS.gene043504:intron 20.0%
!! TTATAATGGTGTATGATATT+TGG + chr2.2:8251890-8251909 MS.gene043504:intron 20.0%
!! TTATGAAAAAACTATTACCA+TGG - chr2.2:8249133-8249152 None:intergenic 20.0%
!! TTCTAGTAATTTATATTCCA+TGG + chr2.2:8250677-8250696 MS.gene043504:intron 20.0%
!! TTGATCATTACAGTTTAAAA+AGG - chr2.2:8250165-8250184 None:intergenic 20.0%
!! TTTAAATTTCAGTAGAATCA+GGG + chr2.2:8251575-8251594 MS.gene043504:intron 20.0%
!! TTTGAAGAAAAACAAAACAT+GGG - chr2.2:8248899-8248918 None:intergenic 20.0%
!!! AAATTATGTTGGTTATGTTT+TGG + chr2.2:8248783-8248802 MS.gene043504:intron 20.0%
!!! AACTTTTATAAATCAACTGA+CGG - chr2.2:8250880-8250899 None:intergenic 20.0%
!!! ATTAGCAATAATTAGTGTTT+TGG + chr2.2:8250044-8250063 MS.gene043504:intron 20.0%
!!! TAAATTCAAGAGATTTTGTT+TGG - chr2.2:8248049-8248068 None:intergenic 20.0%
!!! TTGAATTATTTTCAGGATTA+TGG + chr2.2:8251040-8251059 MS.gene043504:intron 20.0%
!!! TTTTAAATTTCAGTAGAATC+AGG + chr2.2:8251574-8251593 MS.gene043504:intron 20.0%
!!! TTTTTTCATAAAAAATGCCA+TGG + chr2.2:8249142-8249161 MS.gene043504:intron 20.0%
! AAAAGATAATCCCTTCAAAA+TGG + chr2.2:8250519-8250538 MS.gene043504:intron 25.0%
! AACAGAATTGATTATGATCA+TGG - chr2.2:8251253-8251272 None:intergenic 25.0%
! AATCAATTCTGTTATTAAGG+AGG + chr2.2:8251260-8251279 MS.gene043504:intron 25.0%
! ACTCAATAAAGTTTCCAATT+AGG - chr2.2:8248860-8248879 None:intergenic 25.0%
! AGTTTCTATATAAAGGGAAA+AGG - chr2.2:8250006-8250025 None:intergenic 25.0%
! ATGATATTTGGTAAATTAGG+GGG + chr2.2:8251902-8251921 MS.gene043504:intron 25.0%
! CAATTCAATTGTCAAAAGTA+TGG + chr2.2:8248215-8248234 MS.gene043504:CDS 25.0%
! GAGTAAATGTATCATAATCA+CGG - chr2.2:8248404-8248423 None:intergenic 25.0%
! GATCATTACAGTTTAAAAAG+GGG - chr2.2:8250163-8250182 None:intergenic 25.0%
! GGAAAAAAGATTATGTTTGT+TGG + chr2.2:8248572-8248591 MS.gene043504:CDS 25.0%
! GTAAATGATAGTAATAACCA+TGG - chr2.2:8249105-8249124 None:intergenic 25.0%
! TAACTAGCTAGTATAATGTT+TGG - chr2.2:8248432-8248451 None:intergenic 25.0%
! TAATAAATGTGAATCTTCCA+TGG + chr2.2:8249085-8249104 MS.gene043504:intron 25.0%
! TTACTACTAATGATGATTGT+TGG - chr2.2:8250090-8250109 None:intergenic 25.0%
! TTGATTGATGCTTATATCAA+GGG - chr2.2:8250260-8250279 None:intergenic 25.0%
! TTTGACATTTGAATTCAGAT+GGG + chr2.2:8251304-8251323 MS.gene043504:intron 25.0%
!! AACATATTTTCCTCTTACAA+AGG + chr2.2:8249196-8249215 MS.gene043504:intron 25.0%
!! CTCTAAATTTTACAAATTCG+TGG - chr2.2:8251722-8251741 None:intergenic 25.0%
!! GTTTTTAAATCCTTTGTAAG+AGG - chr2.2:8249209-8249228 None:intergenic 25.0%
!! TAAATTTTACAAATTCGTGG+TGG - chr2.2:8251719-8251738 None:intergenic 25.0%
!! TAATGGTAGTGAAATTATGT+TGG + chr2.2:8248772-8248791 MS.gene043504:intron 25.0%
!! TACTTTTGACAATTGAATTG+TGG - chr2.2:8248215-8248234 None:intergenic 25.0%
!! TTAGTGAATGTTTTGTACTA+TGG - chr2.2:8251764-8251783 None:intergenic 25.0%
!! TTATTCTTTAAAAATGTCCC+CGG - chr2.2:8249068-8249087 None:intergenic 25.0%
!! TTCATTTTCATTGAAACTAG+AGG + chr2.2:8250705-8250724 MS.gene043504:intron 25.0%
!! TTTTGACATTTGAATTCAGA+TGG + chr2.2:8251303-8251322 MS.gene043504:intron 25.0%
!! TTTTTGTTCTATTGAGAACA+AGG - chr2.2:8250321-8250340 None:intergenic 25.0%
!!! AATGTGTTTGGTACTTTTTT+AGG + chr2.2:8248516-8248535 MS.gene043504:intron 25.0%
!!! ACAAAAGTGTCACAAAATAT+TGG + chr2.2:8250618-8250637 MS.gene043504:intron 25.0%
!!! AGAAAAAGGCACTATATATT+AGG - chr2.2:8249927-8249946 None:intergenic 25.0%
!!! CAAAAGTGTCACAAAATATT+GGG + chr2.2:8250619-8250638 MS.gene043504:intron 25.0%
!!! GTTTTTTTTTGTTGTGAAAC+CGG - chr2.2:8249897-8249916 None:intergenic 25.0%
!!! TTTGAAGGGATTATCTTTTT+TGG - chr2.2:8250518-8250537 None:intergenic 25.0%
AAAAACTATTACCATGGCAA+TGG - chr2.2:8249127-8249146 None:intergenic 30.0%
AACAGAAGCTATGATAGAAA+TGG - chr2.2:8248317-8248336 None:intergenic 30.0%
AATTCCTAAAAAGAAGACCT+TGG - chr2.2:8251444-8251463 None:intergenic 30.0%
ACTAACCTGAAATGTTATTG+TGG - chr2.2:8248696-8248715 None:intergenic 30.0%
AGAAAATAGACATCACTGAA+AGG + chr2.2:8250729-8250748 MS.gene043504:intron 30.0%
AGAAGAAGTAAAAACAGAGT+CGG - chr2.2:8248014-8248033 None:intergenic 30.0%
AGATGGGATTATATTCAAGA+GGG + chr2.2:8251320-8251339 MS.gene043504:intron 30.0%
AGCATGTATGATTCATTCTT+GGG + chr2.2:8248628-8248647 MS.gene043504:CDS 30.0%
ATTGAATTGTGGATCTATGA+AGG - chr2.2:8248204-8248223 None:intergenic 30.0%
CTCAGTATCAGAAATATAGA+TGG + chr2.2:8248249-8248268 MS.gene043504:CDS 30.0%
CTTGATTGATGCTTATATCA+AGG - chr2.2:8250261-8250280 None:intergenic 30.0%
GGTGTTAAATATAGATCCTA+CGG - chr2.2:8251232-8251251 None:intergenic 30.0%
GTGAGCAATAAAGTATGAAA+CGG + chr2.2:8249018-8249037 MS.gene043504:intron 30.0%
TAGCATGTATGATTCATTCT+TGG + chr2.2:8248627-8248646 MS.gene043504:CDS 30.0%
TATCTCCACAATAACATTTC+AGG + chr2.2:8248688-8248707 MS.gene043504:CDS 30.0%
TCCTATGCAAAGAAAGAAAA+AGG - chr2.2:8249941-8249960 None:intergenic 30.0%
TGAGCAATAAAGTATGAAAC+GGG + chr2.2:8249019-8249038 MS.gene043504:intron 30.0%
TGTTAGATTTAGTGTAGGTT+TGG + chr2.2:8248333-8248352 MS.gene043504:intron 30.0%
TTGAATTGTGGATCTATGAA+GGG - chr2.2:8248203-8248222 None:intergenic 30.0%
! AATAAAGTATGAAACGGGTT+TGG + chr2.2:8249024-8249043 MS.gene043504:intron 30.0%
! ATCCATTTTGTAGTTACTAG+CGG + chr2.2:8248925-8248944 MS.gene043504:intron 30.0%
! GAAACACGTGTTTTCTTTTT+CGG - chr2.2:8249544-8249563 None:intergenic 30.0%
! TATTTCTGATACTGAGTATC+AGG - chr2.2:8248245-8248264 None:intergenic 30.0%
! TTTCTTTTCCTGATCAAAAC+TGG + chr2.2:8249326-8249345 MS.gene043504:intron 30.0%
! TTTTCTCTCATTACTACTAC+TGG - chr2.2:8248102-8248121 None:intergenic 30.0%
!! AATGAAAATGAAAAGTGCCA+TGG - chr2.2:8250697-8250716 None:intergenic 30.0%
!! GATTTTCTAGCAAAGTATAG+GGG + chr2.2:8248551-8248570 MS.gene043504:CDS 30.0%
!! GGATTTTCTAGCAAAGTATA+GGG + chr2.2:8248550-8248569 MS.gene043504:CDS 30.0%
!! GTAGTTTACTGACTTTATGT+AGG - chr2.2:8250600-8250619 None:intergenic 30.0%
!! TAAATATGCCAGTTTTGATC+AGG - chr2.2:8249337-8249356 None:intergenic 30.0%
!! TGGATTTTCTAGCAAAGTAT+AGG + chr2.2:8248549-8248568 MS.gene043504:CDS 30.0%
!!! ATGTATTGGTTTTCAACACA+TGG + chr2.2:8251176-8251195 MS.gene043504:CDS 30.0%
!!! CTTTTTTAGGTTCAATGCAT+TGG + chr2.2:8248529-8248548 MS.gene043504:intron 30.0%
AAACTTGCACAAAGTATGTG+AGG + chr2.2:8251631-8251650 MS.gene043504:intron 35.0%
AAGTTGGACTCAATAAAGAG+TGG + chr2.2:8251133-8251152 MS.gene043504:CDS 35.0%
ACACGATTGATTGTGATTTG+TGG + chr2.2:8250933-8250952 MS.gene043504:intron 35.0%
ACAGCAAATGCAAGAGATTA+AGG - chr2.2:8250480-8250499 None:intergenic 35.0%
ACTATCATTTACCATTGCCA+TGG + chr2.2:8249113-8249132 MS.gene043504:intron 35.0%
AGAACCAAATATAAGACGGA+TGG - chr2.2:8249594-8249613 None:intergenic 35.0%
AGAAGAACATTCCAAGTATC+AGG - chr2.2:8252168-8252187 None:intergenic 35.0%
AGAGAAACACAACCATTCTT+TGG + chr2.2:8251958-8251977 MS.gene043504:CDS 35.0%
AGCTATGATAGAAATGGAGA+AGG - chr2.2:8248311-8248330 None:intergenic 35.0%
AGTAATGAGAGAAAACTTGC+TGG + chr2.2:8248107-8248126 MS.gene043504:CDS 35.0%
ATAACACTACTATACCCTTC+TGG - chr2.2:8252108-8252127 None:intergenic 35.0%
ATTGTACTTTCCCTCTTCTT+TGG + chr2.2:8249290-8249309 MS.gene043504:intron 35.0%
CAAATCACAATCAATCGTGT+TGG - chr2.2:8250933-8250952 None:intergenic 35.0%
CAATTAGGTGAAAGACAGAA+AGG - chr2.2:8248845-8248864 None:intergenic 35.0%
CAGATGGGATTATATTCAAG+AGG + chr2.2:8251319-8251338 MS.gene043504:intron 35.0%
CCACATTGTGTGATAAAAGA+AGG + chr2.2:8250642-8250661 MS.gene043504:intron 35.0%
CTACAAAGGTTTGACAACTT+GGG + chr2.2:8251382-8251401 MS.gene043504:CDS 35.0%
CTCATTACTACTACTGGAAA+TGG - chr2.2:8248096-8248115 None:intergenic 35.0%
CTTGGTATAGAACAAGTGAA+AGG - chr2.2:8248278-8248297 None:intergenic 35.0%
CTTTCACTTGTTCTATACCA+AGG + chr2.2:8248276-8248295 MS.gene043504:CDS 35.0%
CTTTCTGTCTTTCACCTAAT+TGG + chr2.2:8248843-8248862 MS.gene043504:intron 35.0%
CTTTGTAGAACGCAATGAAA+CGG - chr2.2:8251370-8251389 None:intergenic 35.0%
GAATAGAGCTTAATCGCTTA+AGG - chr2.2:8249875-8249894 None:intergenic 35.0%
GAATTAAGGTGACTCAAAGT+AGG + chr2.2:8248375-8248394 MS.gene043504:intron 35.0%
GAGTGAATTCGTCAGAATTA+AGG + chr2.2:8248361-8248380 MS.gene043504:intron 35.0%
GCTTCTGTTAGATTTAGTGT+AGG + chr2.2:8248328-8248347 MS.gene043504:intron 35.0%
GTCACATCTAGAAAATACAC+TGG - chr2.2:8252054-8252073 None:intergenic 35.0%
GTGTGCGGAATAGAAATAAT+TGG - chr2.2:8251069-8251088 None:intergenic 35.0%
TAAGCGATTAAGCTCTATTC+CGG + chr2.2:8249875-8249894 MS.gene043504:intron 35.0%
TACATGCTAGTGAATTGAAC+TGG - chr2.2:8248616-8248635 None:intergenic 35.0%
TCTACAAAGGTTTGACAACT+TGG + chr2.2:8251381-8251400 MS.gene043504:CDS 35.0%
TCTCAAGTAGTGTAGTACTT+TGG + chr2.2:8250769-8250788 MS.gene043504:intron 35.0%
! CAAACCAAGGTCTTCTTTTT+AGG + chr2.2:8251437-8251456 MS.gene043504:CDS 35.0%
! CAATAAAGAGTGGTAATGCA+TGG + chr2.2:8251143-8251162 MS.gene043504:CDS 35.0%
! CCTTCTTTTATCACACAATG+TGG - chr2.2:8250645-8250664 None:intergenic 35.0%
! GCCTTTTTCTTTCTTTGCAT+AGG + chr2.2:8249937-8249956 MS.gene043504:intron 35.0%
! GTGAATGTTTTGTACTATGG+TGG - chr2.2:8251761-8251780 None:intergenic 35.0%
!! ACACATAGATTTTGGCATAC+TGG - chr2.2:8251695-8251714 None:intergenic 35.0%
!! GGGAAAAGGTATTGACAATT+TGG - chr2.2:8249992-8250011 None:intergenic 35.0%
!!! GAGAAGGAATACCTTGTTTT+TGG - chr2.2:8248656-8248675 None:intergenic 35.0%
AACACGTGTTTCTCAGACTA+TGG + chr2.2:8249552-8249571 MS.gene043504:intron 40.0%
ACAAAACATGGGTTCACACA+AGG - chr2.2:8248888-8248907 None:intergenic 40.0%
ACAACTTACTCGTAATGCAC+AGG - chr2.2:8251470-8251489 None:intergenic 40.0%
ACGTGCAAAGAAGAAAAGGT+TGG - chr2.2:8250140-8250159 None:intergenic 40.0%
AGAAAAGATGCACATCCAGA+AGG + chr2.2:8252090-8252109 MS.gene043504:CDS 40.0%
AGGAAAAGTCATGAAAGCAG+AGG - chr2.2:8250580-8250599 None:intergenic 40.0%
ATGTCTACGTTCCAAAGAAG+AGG - chr2.2:8249304-8249323 None:intergenic 40.0%
ATTCTTGGGTGCCAAAAACA+AGG + chr2.2:8248642-8248661 MS.gene043504:CDS 40.0%
CAAAGAACACATCACTTGCA+TGG - chr2.2:8248822-8248841 None:intergenic 40.0%
CAAATATAAGACGGATGGAG+CGG - chr2.2:8249589-8249608 None:intergenic 40.0%
CAAGAAGAAGGATCATAGAG+AGG - chr2.2:8248179-8248198 None:intergenic 40.0%
CACCAATGACTACAATCTGT+TGG - chr2.2:8252132-8252151 None:intergenic 40.0%
CGTTTCATTGCGTTCTACAA+AGG + chr2.2:8251368-8251387 MS.gene043504:CDS 40.0%
GAAAAGATGCACATCCAGAA+GGG + chr2.2:8252091-8252110 MS.gene043504:CDS 40.0%
GATCATAGAGAGGATACGAA+GGG - chr2.2:8248169-8248188 None:intergenic 40.0%
GGAGAAGGAGATATATACCT+TGG - chr2.2:8248296-8248315 None:intergenic 40.0%
GGGTACTTCAAACCAAAGAA+TGG - chr2.2:8251973-8251992 None:intergenic 40.0%
TCCATGTTGGTGTGAAACAA+TGG + chr2.2:8248992-8249011 MS.gene043504:intron 40.0%
TCCATTGTTTCACACCAACA+TGG - chr2.2:8248996-8249015 None:intergenic 40.0%
TCTATGAAGGGACAAGAAGA+AGG - chr2.2:8248191-8248210 None:intergenic 40.0%
TCTATTCCGCACACCATATT+TGG + chr2.2:8251075-8251094 MS.gene043504:CDS 40.0%
TGCCAACAGATTGTAGTCAT+TGG + chr2.2:8252127-8252146 MS.gene043504:CDS 40.0%
TGGGATTATATTCAAGAGGG+TGG + chr2.2:8251323-8251342 MS.gene043504:CDS 40.0%
TGTAAATCCAGCTCAAACCA+AGG + chr2.2:8251424-8251443 MS.gene043504:CDS 40.0%
TGTCTACGTTCCAAAGAAGA+GGG - chr2.2:8249303-8249322 None:intergenic 40.0%
TTAAAAATGTCCCCGGCAAA+AGG - chr2.2:8249061-8249080 None:intergenic 40.0%
TTATATTCAAGAGGGTGGCA+AGG + chr2.2:8251328-8251347 MS.gene043504:CDS 40.0%
TTCACTATTACCCAAGCCAT+TGG - chr2.2:8252006-8252025 None:intergenic 40.0%
! AAAGTATGAAACGGGTTTGG+TGG + chr2.2:8249027-8249046 MS.gene043504:intron 40.0%
! GTCATGTGGTCCATTTTGAA+GGG - chr2.2:8250532-8250551 None:intergenic 40.0%
! TAACTGGTTTCGTGGCAAAT+GGG + chr2.2:8248136-8248155 MS.gene043504:CDS 40.0%
! TCTCTCATGCATGATTTTGC+TGG - chr2.2:8251943-8251962 None:intergenic 40.0%
! TGGTTTTCAACACATGGCAT+TGG + chr2.2:8251182-8251201 MS.gene043504:CDS 40.0%
! TGTCATGTGGTCCATTTTGA+AGG - chr2.2:8250533-8250552 None:intergenic 40.0%
! TTTGACAACTTGGGCTAGAT+GGG + chr2.2:8251391-8251410 MS.gene043504:CDS 40.0%
!! AACTTGCTGGAAGATGTAAC+TGG + chr2.2:8248120-8248139 MS.gene043504:CDS 40.0%
!! GATAGCACTTTGCTTTGAGA+AGG - chr2.2:8248672-8248691 None:intergenic 40.0%
!! TTTTCAACACATGGCATTGG+TGG + chr2.2:8251185-8251204 MS.gene043504:CDS 40.0%
!!! GTTTTCTTTTTCGGTCTAGC+TGG - chr2.2:8249535-8249554 None:intergenic 40.0%
ACCTTGACCGTGAGAACATT+GGG + chr2.2:8251101-8251120 MS.gene043504:CDS 45.0%
ATGCAGCTCTCTTTGGTTGA+AGG + chr2.2:8252190-8252209 MS.gene043504:CDS 45.0%
ATGGAGAGGGATGGATGTAT+TGG + chr2.2:8251162-8251181 MS.gene043504:CDS 45.0%
CAAAATGGACCACATGACAC+TGG + chr2.2:8250534-8250553 MS.gene043504:intron 45.0%
CGCCGCTAGTAACTACAAAA+TGG - chr2.2:8248930-8248949 None:intergenic 45.0%
CGCTCCATCCGTCTTATATT+TGG + chr2.2:8249587-8249606 MS.gene043504:intron 45.0%
CGGTCAAGGTCTACCAAATA+TGG - chr2.2:8251091-8251110 None:intergenic 45.0%
CTTCTTCATGCAGCTCTCTT+TGG + chr2.2:8252183-8252202 MS.gene043504:CDS 45.0%
GGATCATAGAGAGGATACGA+AGG - chr2.2:8248170-8248189 None:intergenic 45.0%
GGTAAATTAGGGGGAGAGAT+TGG + chr2.2:8251911-8251930 MS.gene043504:intron 45.0%
GGTCTACCAAATATGGTGTG+CGG - chr2.2:8251084-8251103 None:intergenic 45.0%
TCACACCACATTGTTGACAC+TGG - chr2.2:8250963-8250982 None:intergenic 45.0%
TCACTGAAAGGAGTTGAGTG+AGG + chr2.2:8250741-8250760 MS.gene043504:intron 45.0%
TTCCAAGTATCAGGAAGTCC+TGG - chr2.2:8252159-8252178 None:intergenic 45.0%
TTCCAGGACTTCCTGATACT+TGG + chr2.2:8252154-8252173 MS.gene043504:CDS 45.0%
! AAGAAGACCTTGGTTTGAGC+TGG - chr2.2:8251434-8251453 None:intergenic 45.0%
! AAGAGTGGTAATGCATGGAG+AGG + chr2.2:8251148-8251167 MS.gene043504:CDS 45.0%
! AGAGTGGTAATGCATGGAGA+GGG + chr2.2:8251149-8251168 MS.gene043504:CDS 45.0%
! CAGAGGAGTGCATCTGAATT+AGG - chr2.2:8250563-8250582 None:intergenic 45.0%
! GGAAGATGTAACTGGTTTCG+TGG + chr2.2:8248128-8248147 MS.gene043504:CDS 45.0%
! GGTAGTTGTTGCTGTTGTGT+TGG - chr2.2:8248075-8248094 None:intergenic 45.0%
! GTAACTGGTTTCGTGGCAAA+TGG + chr2.2:8248135-8248154 MS.gene043504:CDS 45.0%
! GTGGTGGCACACATAGATTT+TGG - chr2.2:8251703-8251722 None:intergenic 45.0%
! GTTACTAGCGGCGAATTTTG+TGG + chr2.2:8248937-8248956 MS.gene043504:intron 45.0%
! GTTTGACAACTTGGGCTAGA+TGG + chr2.2:8251390-8251409 MS.gene043504:CDS 45.0%
! TGTAGTCATTGGTGTCTTCC+AGG + chr2.2:8252138-8252157 MS.gene043504:CDS 45.0%
!! CATTGGGCGTGTTCTAAAGT+TGG + chr2.2:8251117-8251136 MS.gene043504:CDS 45.0%
!! ATTTAAAAAATACATTTATT+GGG + chr2.2:8249235-8249254 MS.gene043504:intron 5.0%
!! TATTTAAAAAATACATTTAT+TGG + chr2.2:8249234-8249253 MS.gene043504:intron 5.0%
!!! ATATTTAATTTATCTTTTTT+TGG + chr2.2:8249350-8249369 MS.gene043504:intron 5.0%
!!! TATTAATATTATTTTCAAAA+AGG - chr2.2:8250434-8250453 None:intergenic 5.0%
AATGGACCACATGACACTGG+TGG + chr2.2:8250537-8250556 MS.gene043504:intron 50.0%
ACGGTTGTGCATTTCCAGTG+TGG - chr2.2:8251213-8251232 None:intergenic 50.0%
ATTAGGCCACCAGTGTCATG+TGG - chr2.2:8250546-8250565 None:intergenic 50.0%
CACTGGAAATGCACAACCGT+AGG + chr2.2:8251213-8251232 MS.gene043504:intron 50.0%
CGGTTGTGCATTTCCAGTGT+GGG - chr2.2:8251212-8251231 None:intergenic 50.0%
GAACACGCCCAATGTTCTCA+CGG - chr2.2:8251111-8251130 None:intergenic 50.0%
GACCTTGACCGTGAGAACAT+TGG + chr2.2:8251100-8251119 MS.gene043504:CDS 50.0%
GGGGACGTGCAAAGAAGAAA+AGG - chr2.2:8250144-8250163 None:intergenic 50.0%
GTGTGCCAGTGTCAACAATG+TGG + chr2.2:8250955-8250974 MS.gene043504:intron 50.0%
! GTGGAGGCTTTTCCTTTTGC+CGG + chr2.2:8249046-8249065 MS.gene043504:intron 50.0%
! TGGAGGCTTTTCCTTTTGCC+GGG + chr2.2:8249047-8249066 MS.gene043504:intron 50.0%
! TGGTAATGCATGGAGAGGGA+TGG + chr2.2:8251153-8251172 MS.gene043504:CDS 50.0%
!! GTATGAAACGGGTTTGGTGG+AGG + chr2.2:8249030-8249049 MS.gene043504:intron 50.0%
GCCCAATGTTCTCACGGTCA+AGG - chr2.2:8251105-8251124 None:intergenic 55.0%
! GGAGGCTTTTCCTTTTGCCG+GGG + chr2.2:8249048-8249067 MS.gene043504:intron 55.0%
!! TGGCATTGGTGGACCCACAC+TGG + chr2.2:8251196-8251215 MS.gene043504:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 8247996 8252209 8247996 ID=MS.gene043504
chr2.2 mRNA 8247996 8252209 8247996 ID=MS.gene043504.t1;Parent=MS.gene043504
chr2.2 exon 8247996 8248297 8247996 ID=MS.gene043504.t1.exon1;Parent=MS.gene043504.t1
chr2.2 CDS 8247996 8248297 8247996 ID=cds.MS.gene043504.t1;Parent=MS.gene043504.t1
chr2.2 exon 8248538 8248709 8248538 ID=MS.gene043504.t1.exon2;Parent=MS.gene043504.t1
chr2.2 CDS 8248538 8248709 8248538 ID=cds.MS.gene043504.t1;Parent=MS.gene043504.t1
chr2.2 exon 8251055 8251230 8251055 ID=MS.gene043504.t1.exon3;Parent=MS.gene043504.t1
chr2.2 CDS 8251055 8251230 8251055 ID=cds.MS.gene043504.t1;Parent=MS.gene043504.t1
chr2.2 exon 8251322 8251480 8251322 ID=MS.gene043504.t1.exon4;Parent=MS.gene043504.t1
chr2.2 CDS 8251322 8251480 8251322 ID=cds.MS.gene043504.t1;Parent=MS.gene043504.t1
chr2.2 exon 8251921 8252209 8251921 ID=MS.gene043504.t1.exon5;Parent=MS.gene043504.t1
chr2.2 CDS 8251921 8252209 8251921 ID=cds.MS.gene043504.t1;Parent=MS.gene043504.t1
Gene Sequence

>MS.gene043504

ATGGGTTTTCTGTTACCGACTCTGTTTTTACTTCTTCTGTTTTCTTCACACCAAACAAAATCTCTTGAATTTAACTCCAACACAACAGCAACAACTACCATTTCCAGTAGTAGTAATGAGAGAAAACTTGCTGGAAGATGTAACTGGTTTCGTGGCAAATGGGTCTATGACCCTTCGTATCCTCTCTATGATCCTTCTTCTTGTCCCTTCATAGATCCACAATTCAATTGTCAAAAGTATGGCAGACCTGATACTCAGTATCAGAAATATAGATGGCAACCTTTCACTTGTTCTATACCAAGGTATATATCTCCTTCTCCATTTCTATCATAGCTTCTGTTAGATTTAGTGTAGGTTTGGTATCAGAGTGAATTCGTCAGAATTAAGGTGACTCAAAGTAGGTCACCGTGATTATGATACATTTACTCTGATACCAAACATTATACTAGCTAGTTATATTGTATAACTAATTTTTATTTCTAATAGTTTTAAGTGTGATAAATGTAAGTTAGAAAATGAAAATGTGTTTGGTACTTTTTTAGGTTCAATGCATTGGATTTTCTAGCAAAGTATAGGGGAAAAAAGATTATGTTTGTTGGTGATTCTTTGAGCTTAAACCAGTTCAATTCACTAGCATGTATGATTCATTCTTGGGTGCCAAAAACAAGGTATTCCTTCTCAAAGCAAAGTGCTATCTCCACAATAACATTTCAGGTTAGTTTTTGTATTTCTATGTTTTTTTTTCTTTAATTTCGATTTTTTGATACTTTTGAATTTAATGGTAGTGAAATTATGTTGGTTATGTTTTGGTGTGTAATTGTGTCCATGCAAGTGATGTGTTCTTTGCCTTTCTGTCTTTCACCTAATTGGAAACTTTATTGAGTAATTGCCTTGTGTGAACCCATGTTTTGTTTTTCTTCAAATCTACGATCCATTTTGTAGTTACTAGCGGCGAATTTTGTGGTCAAATTATTATCTTATTTATTTTTATTATTTTCCATGTTGGTGTGAAACAATGGAGCGTGAGCAATAAAGTATGAAACGGGTTTGGTGGAGGCTTTTCCTTTTGCCGGGGACATTTTTAAAGAATAATAAATGTGAATCTTCCATGGTTATTACTATCATTTACCATTGCCATGGTAATAGTTTTTTCATAAAAAATGCCATGGTAATTATAGATAGTAATTCATGTTTTCTATTAACATATTTTCCTCTTACAAAGGATTTAAAAACAATAATATTTAAAAAATACATTTATTGGGGGGAAATATAAATATATTAACATTTTCATATGATTGTACTTTCCCTCTTCTTTGGAACGTAGACATTATTTCTTTTCCTGATCAAAACTGGCATATTTAATTTATCTTTTTTTGGTTGTTATGATTATTTAATAGATGCTTGTTAGTATTCTTCAAACTTTTATCAAAGTATATAAATTTTAAAATATTTTTTTTATACAAATATTCGATATATTTTAAAAGTTTAATTTCAATGTAATTTTTAATAAATATTTTAAAAGTTTAATTTCTTTAACCAGCTAGACCGAAAAAGAAAACACGTGTTTCTCAGACTATGGAATAGATGCATCCGCTCCATCCGTCTTATATTTGGTTCTATTTAAGTTTGCATTAAGAATGTACCAAAAATGAAATTTGTATTGAAAGAATGATTAATAGCGTTGATTTAAATGATAAATATGTGTCATTTACTAAACATTCTTATTAATTATAATTAGTAAAATAATTATAGATAATTGAAATGCAGTAAATAAATTATATGTTAGTGGAAAAGAATAATACAATATTAGTATGGTAAAAAAAGATACAAACTAGTGGTTGAAGTCTTGAACGAGAGTTTCGTCATTTATATGTCACCTTAAGCGATTAAGCTCTATTCCGGTTTCACAACAAAAAAAAACTATTGATCCTAATATATAGTGCCTTTTTCTTTCTTTGCATAGGAAATGCTCCAATAAAATTATATTCATGATCCAAATTGTCAATACCTTTTCCCTTTATATAGAAACTTTAGTAACAAATTTCTTAATTAGCAATAATTAGTGTTTTGGCATCTAAGTTTTCTAGCACTCCAACAATCATCATTAGTAGTAACTAAATTATGAACACAACGTTACTCAGCCAACCTTTTCTTCTTTGCACGTCCCCTTTTTAAACTGTAATGATCAATAATTTGATACTACTATCTAGTTTTTCTAACTTCAAGAAATATCTGATTGAATACATGCTTTATTATGTTTTCCCTTGATATAAGCATCAATCAAGCTATTCACATTAAAAAAATAGGAGTAAAAAATGTGTTCCTTGTTCTCAATAGAACAAAAAATAAAATTTGAAGTGGGCCATATGAATTAAATTGTAGTACTAACAGTAGTATATACTATATATTTTATTCATTATAGGCTTGATGACATTCCTTTTTGAAAATAATATTAATACTGCATAAAATTAGTAATTTCTTCCTTAATCTCTTGCATTTGCTGTCTTTCAATGTTTGTACCAAAAAAGATAATCCCTTCAAAATGGACCACATGACACTGGTGGCCTAATTCAGATGCACTCCTCTGCTTTCATGACTTTTCCTACATAAAGTCAGTAAACTACAAAAGTGTCACAAAATATTGGGCCACATTGTGTGATAAAAGAAGGAATTTTCCTTTCTTCTAGTAATTTATATTCCATGGCACTTTTCATTTTCATTGAAACTAGAGGCAGAAAATAGACATCACTGAAAGGAGTTGAGTGAGGAAATTTCTCAAGTAGTGTAGTACTTTGGTAGAAAGTTATTTAAATAGAAATTAGATGAAAATTATGTACAATTATTATATGTAAGGAGTTTTTACACTCACAATCAATTTTAACCGTCAGTTGATTTATAAAAGTTTGACTTTTATCATAATTACTTCTAAAACGACCAACACGATTGATTGTGATTTGTGGTGTGCCAGTGTCAACAATGTGGTGTGATAGTGATTTAAACTTAATATTCTGTCGAAAAAAAAAACTAAACTTAATAATTATTATTTGAATTATTTTCAGGATTATGGTCTCCAATTATTTCTATTCCGCACACCATATTTGGTAGACCTTGACCGTGAGAACATTGGGCGTGTTCTAAAGTTGGACTCAATAAAGAGTGGTAATGCATGGAGAGGGATGGATGTATTGGTTTTCAACACATGGCATTGGTGGACCCACACTGGAAATGCACAACCGTAGGATCTATATTTAACACCATGATCATAATCAATTCTGTTATTAAGGAGGTGATTAATTAGTTGTTGTTTTTTTGACATTTGAATTCAGATGGGATTATATTCAAGAGGGTGGCAAGGTGTACAAAGACATGAACCGTTTCATTGCGTTCTACAAAGGTTTGACAACTTGGGCTAGATGGGTTAACATCAATGTAAATCCAGCTCAAACCAAGGTCTTCTTTTTAGGAATTTCTCCTGTGCATTACGAGTAAGTTGTTCTATCTACTTCAATTTAATATATTATCTAGTGCGTGTTTGAATTCACAATGTTTTTTTACAGAATTACGGTGTACTATCGTGATTTTAAATTTCAGTAGAATCAGGGTCACTTTACTTTTGTTACACTTGCTATGACTTCAAACTTGCACAAAGTATGTGAGGTTTTACAAAAGAAATCATTAGAATGTTTGGTATCGCAGCCAGTATGCCAAAATCTATGTGTGCCACCACGAATTTGTAAAATTTAGAGTGTAGTTTTTGCAAAACCACCATAGTACAAAACATTCACTAAACTTGCTTATGTCAAAAGTAATTTTCGTAAAAGTGATAAAAAAAAAGTTATTCTTTTTGTTACTAAATTGTGTGCCAATTACTAGACTAAGTTTTATCAACTATATTTATAATGGTGTATGATATTTGGTAAATTAGGGGGAGAGATTGGAATCAACCAGCAAAATCATGCATGAGAGAAACACAACCATTCTTTGGTTTGAAGTACCCAGCAGGAACACCAATGGCTTGGGTAATAGTGAATAAAGTATTGAGCAGATTAAAGAAGCCAGTGTATTTTCTAGATGTGACAACATTGTCACAGTACAGAAAAGATGCACATCCAGAAGGGTATAGTAGTGTTATGCCAACAGATTGTAGTCATTGGTGTCTTCCAGGACTTCCTGATACTTGGAATGTTCTTCTTCATGCAGCTCTCTTTGGTTGA
Protein sequence

>MS.gene043504.t1

MGFLLPTLFLLLLFSSHQTKSLEFNSNTTATTTISSSSNERKLAGRCNWFRGKWVYDPSYPLYDPSSCPFIDPQFNCQKYGRPDTQYQKYRWQPFTCSIPRFNALDFLAKYRGKKIMFVGDSLSLNQFNSLACMIHSWVPKTRYSFSKQSAISTITFQDYGLQLFLFRTPYLVDLDRENIGRVLKLDSIKSGNAWRGMDVLVFNTWHWWTHTGNAQPWDYIQEGGKVYKDMNRFIAFYKGLTTWARWVNINVNPAQTKVFFLGISPVHYEGRDWNQPAKSCMRETQPFFGLKYPAGTPMAWVIVNKVLSRLKKPVYFLDVTTLSQYRKDAHPEGYSSVMPTDCSHWCLPGLPDTWNVLLHAALFG