AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031625

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031625.t1 MTR_4g079700 97.534 365 5 1 1 361 1 365 0.0 748
MS.gene031625.t1 MTR_2g091175 75.783 351 77 1 17 359 16 366 0.0 566
MS.gene031625.t1 MTR_2g015720 59.322 354 139 4 10 360 8 359 7.50e-154 437
MS.gene031625.t1 MTR_4g087730 59.748 318 127 1 43 359 45 362 2.03e-142 408
MS.gene031625.t1 MTR_4g088030 58.255 321 133 1 40 359 30 350 9.52e-140 401
MS.gene031625.t1 MTR_3g049330 55.937 320 138 2 43 359 31 350 3.90e-135 389
MS.gene031625.t1 MTR_2g073510 56.289 318 135 3 43 356 33 350 1.22e-131 380
MS.gene031625.t1 MTR_5g034810 51.532 359 159 4 4 359 6 352 1.23e-130 378
MS.gene031625.t1 MTR_3g021060 51.351 333 155 2 30 361 39 365 1.29e-128 373
MS.gene031625.t1 MTR_3g035830 50.000 332 159 2 31 361 25 350 1.03e-124 363
MS.gene031625.t1 MTR_3g021090 50.000 326 156 2 31 355 25 344 8.13e-121 355
MS.gene031625.t1 MTR_5g071350 46.499 357 166 5 28 359 43 399 3.51e-117 345
MS.gene031625.t1 MTR_3g464570 49.254 335 153 4 42 359 42 376 1.67e-116 343
MS.gene031625.t1 MTR_2g073500 47.401 327 152 6 43 359 29 345 3.93e-105 313
MS.gene031625.t1 MTR_1g111010 45.732 328 155 10 40 359 29 341 3.68e-91 277
MS.gene031625.t1 MTR_0136s0010 38.859 368 198 6 16 359 145 509 1.86e-88 276
MS.gene031625.t1 MTR_1g050448 39.881 336 180 7 38 359 48 375 7.63e-88 270
MS.gene031625.t1 MTR_7g093450 40.643 342 176 7 43 359 248 587 5.92e-86 271
MS.gene031625.t1 MTR_3g464570 47.601 271 125 4 106 359 2 272 1.02e-84 258
MS.gene031625.t1 MTR_1g112210 36.842 361 194 10 23 359 32 382 1.30e-83 259
MS.gene031625.t1 MTR_1g112210 37.673 361 178 12 23 359 32 369 1.92e-83 258
MS.gene031625.t1 MTR_4g121560 37.901 343 186 8 42 359 113 453 5.52e-80 252
MS.gene031625.t1 MTR_5g082880 38.329 347 184 9 43 359 165 511 6.54e-80 253
MS.gene031625.t1 MTR_4g126950 33.242 364 219 7 18 359 85 446 1.07e-79 251
MS.gene031625.t1 MTR_3g070900 38.372 344 185 9 43 359 106 449 2.12e-79 250
MS.gene031625.t1 MTR_5g059400 36.782 348 192 7 38 359 170 515 2.50e-79 252
MS.gene031625.t1 MTR_1g116130 36.782 348 192 7 38 359 170 515 2.50e-79 252
MS.gene031625.t1 MTR_7g109550 36.905 336 188 8 43 359 137 467 4.29e-78 248
MS.gene031625.t1 MTR_3g071650 37.318 343 188 9 43 359 89 430 7.80e-76 241
MS.gene031625.t1 MTR_5g083260 36.182 351 199 7 34 359 74 424 8.44e-75 238
MS.gene031625.t1 MTR_5g033800 32.584 356 216 5 27 359 129 483 1.38e-74 239
MS.gene031625.t1 MTR_4g016940 34.795 342 198 7 43 359 86 427 7.94e-74 235
MS.gene031625.t1 MTR_8g069790 34.870 347 202 6 36 359 171 516 9.76e-74 238
MS.gene031625.t1 MTR_8g463790 36.000 350 188 10 39 359 67 409 1.08e-73 234
MS.gene031625.t1 MTR_1g058380 35.925 373 205 10 12 359 31 394 3.07e-70 225
MS.gene031625.t1 MTR_4g014440 31.854 383 231 9 8 361 28 409 5.08e-69 222
MS.gene031625.t1 MTR_3g090120 37.609 343 189 8 37 359 122 459 2.77e-68 222
MS.gene031625.t1 MTR_7g094750 35.756 344 192 9 43 359 91 432 3.09e-68 221
MS.gene031625.t1 MTR_7g070060 31.620 389 232 11 1 359 25 409 8.20e-68 219
MS.gene031625.t1 MTR_2g022560 34.694 343 198 7 40 357 94 435 2.12e-67 219
MS.gene031625.t1 MTR_4g014430 34.795 342 197 8 42 359 107 446 1.04e-66 217
MS.gene031625.t1 MTR_7g068920 35.965 342 194 7 43 359 80 421 1.69e-66 216
MS.gene031625.t1 MTR_8g093530 32.344 337 198 9 43 359 90 416 2.05e-57 192
MS.gene031625.t1 MTR_5g033800 30.952 252 162 3 27 266 129 380 4.11e-47 164
MS.gene031625.t1 MTR_7g070060 30.909 275 175 7 1 264 25 295 5.04e-47 162
MS.gene031625.t1 MTR_5g069320 36.111 288 110 9 90 352 148 386 1.36e-46 163
MS.gene031625.t1 MTR_7g094750 34.454 238 142 4 43 266 91 328 8.40e-45 157
MS.gene031625.t1 MTR_7g070060 29.693 293 178 7 95 359 15 307 5.88e-41 146
MS.gene031625.t1 MTR_4g092830 29.395 347 205 13 43 355 48 388 7.15e-38 140
MS.gene031625.t1 MTR_6g016240 30.352 369 218 12 27 360 53 417 2.46e-37 139
MS.gene031625.t1 MTR_6g061710 27.869 366 209 11 37 359 161 514 1.87e-36 138
MS.gene031625.t1 MTR_1g076690 29.577 355 197 14 43 355 223 566 3.94e-35 135
MS.gene031625.t1 MTR_8g093870 29.752 363 216 15 28 359 118 472 1.33e-34 132
MS.gene031625.t1 MTR_5g035080 30.899 356 197 16 43 359 75 420 8.75e-34 130
MS.gene031625.t1 MTR_6g016880 27.500 400 239 13 1 359 30 419 1.27e-32 126
MS.gene031625.t1 MTR_5g035090 29.143 350 210 13 43 359 83 427 5.44e-32 125
MS.gene031625.t1 MTR_5g035150 29.429 350 213 12 42 359 84 431 1.19e-31 124
MS.gene031625.t1 MTR_7g019120 26.171 363 227 8 30 359 66 420 7.81e-31 122
MS.gene031625.t1 MTR_6g016875 26.781 351 217 10 43 359 64 408 2.42e-30 120
MS.gene031625.t1 MTR_7g033230 28.232 379 225 14 18 359 241 609 1.91e-29 119
MS.gene031625.t1 MTR_5g035070 28.329 353 213 11 42 359 86 433 2.93e-29 117
MS.gene031625.t1 MTR_8g104970 28.406 345 212 10 43 355 120 461 3.25e-29 117
MS.gene031625.t1 MTR_4g125060 26.882 372 211 14 27 359 122 471 3.25e-29 117
MS.gene031625.t1 MTR_7g033230 28.232 379 225 14 18 359 311 679 3.43e-29 119
MS.gene031625.t1 MTR_4g125060 27.171 357 205 13 42 359 170 510 3.54e-29 118
MS.gene031625.t1 MTR_4g125060 26.810 373 212 14 26 359 189 539 4.51e-29 118
MS.gene031625.t1 MTR_2g016060 27.350 351 223 9 36 355 47 396 5.02e-29 116
MS.gene031625.t1 MTR_8g027900 27.198 364 199 15 43 359 211 555 7.39e-27 112
MS.gene031625.t1 MTR_3g021150 42.982 114 64 1 227 339 1 114 1.71e-26 102
MS.gene031625.t1 MTR_3g109840 28.814 354 204 15 43 359 73 415 4.18e-26 108
MS.gene031625.t1 MTR_6g007777 27.066 351 215 10 42 359 115 457 1.13e-25 107
MS.gene031625.t1 MTR_8g099300 24.438 356 218 9 40 355 71 415 1.23e-23 101
MS.gene031625.t1 MTR_7g019140 40.196 102 61 0 30 131 66 167 3.05e-19 84.3
MS.gene031625.t1 MTR_5g035120 41.758 91 53 0 43 133 75 165 1.94e-16 76.6
MS.gene031625.t1 MTR_3g021080 33.000 100 47 2 261 359 99 179 1.74e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031625.t1 AT2G42570 63.855 332 119 1 29 359 33 364 1.73e-166 469
MS.gene031625.t1 AT2G31110 58.659 358 144 3 6 360 7 363 2.89e-163 461
MS.gene031625.t1 AT1G29050 61.491 322 123 1 40 360 58 379 1.93e-155 442
MS.gene031625.t1 AT2G34070 61.250 320 122 2 43 360 65 384 9.73e-150 427
MS.gene031625.t1 AT3G14850 57.009 321 134 3 40 356 31 351 1.41e-133 385
MS.gene031625.t1 AT1G78710 50.440 341 162 5 23 359 19 356 1.22e-124 363
MS.gene031625.t1 AT2G30900 50.769 325 154 4 43 361 43 367 2.90e-120 352
MS.gene031625.t1 AT2G34070 61.200 250 95 2 113 360 1 250 1.84e-113 330
MS.gene031625.t1 AT5G58600 48.214 336 156 5 42 359 65 400 8.16e-110 327
MS.gene031625.t1 AT2G31110 56.693 254 107 2 6 257 7 259 2.12e-106 313
MS.gene031625.t1 AT2G30010 42.328 378 194 5 6 359 19 396 1.86e-103 310
MS.gene031625.t1 AT2G30010 42.328 378 194 5 6 359 62 439 4.26e-103 311
MS.gene031625.t1 AT2G31110 63.462 208 75 1 154 360 8 215 3.45e-102 300
MS.gene031625.t1 AT3G14850 56.048 248 105 3 113 356 1 248 3.33e-96 286
MS.gene031625.t1 AT1G78710 41.944 360 141 7 4 359 1 296 2.95e-93 281
MS.gene031625.t1 AT5G06700 41.096 365 190 6 19 359 231 594 2.05e-90 283
MS.gene031625.t1 AT3G54260 39.940 333 175 6 43 359 51 374 7.85e-86 265
MS.gene031625.t1 AT3G12060 39.474 342 182 5 42 359 195 535 1.59e-85 269
MS.gene031625.t1 AT2G30900 52.511 219 102 2 43 259 43 261 9.29e-84 256
MS.gene031625.t1 AT3G62390 38.166 338 188 6 43 361 138 473 3.42e-81 256
MS.gene031625.t1 AT5G58600 50.667 225 109 2 42 264 65 289 5.34e-79 244
MS.gene031625.t1 AT5G49340 36.544 353 181 7 41 359 98 441 5.98e-79 249
MS.gene031625.t1 AT3G55990 35.792 366 206 9 21 359 120 483 4.86e-78 248
MS.gene031625.t1 AT5G20590 35.882 340 194 7 43 359 141 479 1.20e-77 247
MS.gene031625.t1 AT1G73140 37.209 344 186 10 42 359 66 405 1.87e-75 239
MS.gene031625.t1 AT2G40160 35.714 350 193 9 43 361 77 425 2.42e-75 239
MS.gene031625.t1 AT2G40160 35.756 344 189 9 43 355 77 419 7.77e-75 238
MS.gene031625.t1 AT1G60790 36.047 344 192 7 43 359 190 532 2.31e-74 240
MS.gene031625.t1 AT2G40150 34.571 350 196 8 43 359 71 420 6.40e-74 235
MS.gene031625.t1 AT3G11030 35.694 353 199 9 34 359 97 448 2.43e-72 232
MS.gene031625.t1 AT5G01620 34.571 350 202 8 35 359 102 449 8.33e-72 231
MS.gene031625.t1 AT5G01620 34.571 350 202 8 35 359 102 449 8.33e-72 231
MS.gene031625.t1 AT5G01620 34.571 350 202 8 35 359 110 457 1.16e-71 230
MS.gene031625.t1 AT2G40320 34.521 365 214 8 20 359 58 422 2.52e-71 229
MS.gene031625.t1 AT5G01360 34.985 343 197 8 43 359 36 378 1.40e-70 225
MS.gene031625.t1 AT5G01360 34.985 343 197 8 43 359 92 434 5.44e-70 225
MS.gene031625.t1 AT2G38320 32.216 388 233 9 1 359 18 404 7.26e-70 224
MS.gene031625.t1 AT5G19160 34.109 387 203 10 19 357 60 442 1.11e-69 226
MS.gene031625.t1 AT3G06080 35.380 342 193 8 43 357 103 443 7.57e-68 221
MS.gene031625.t1 AT1G48880 35.310 371 215 9 1 355 74 435 2.56e-66 216
MS.gene031625.t1 AT5G06230 36.000 350 193 10 39 359 17 364 7.71e-66 213
MS.gene031625.t1 AT5G06230 36.000 350 193 10 39 359 58 405 2.21e-65 213
MS.gene031625.t1 AT3G11570 36.223 323 183 6 59 359 98 419 2.20e-64 211
MS.gene031625.t1 AT2G38320 31.761 318 189 7 70 359 4 321 3.77e-56 186
MS.gene031625.t1 AT3G62390 36.709 237 138 3 43 267 138 374 6.27e-55 186
MS.gene031625.t1 AT2G40160 37.179 234 135 5 43 264 77 310 2.89e-52 176
MS.gene031625.t1 AT5G20590 35.294 238 140 5 43 266 141 378 2.82e-51 175
MS.gene031625.t1 AT5G19160 33.935 277 154 5 19 266 60 336 3.71e-49 169
MS.gene031625.t1 AT3G06080 35.714 238 139 4 43 266 103 340 1.75e-46 162
MS.gene031625.t1 AT5G06230 35.081 248 141 5 39 266 58 305 1.99e-44 155
MS.gene031625.t1 AT2G14530 29.973 367 215 10 28 359 47 406 5.59e-42 151
MS.gene031625.t1 AT5G64470 29.799 349 212 12 36 355 46 390 6.16e-40 145
MS.gene031625.t1 AT5G64470 29.775 356 209 14 36 355 46 396 4.45e-38 141
MS.gene031625.t1 AT1G70230 29.412 340 210 10 43 359 79 411 5.37e-38 141
MS.gene031625.t1 AT5G51640 27.249 389 227 12 16 359 111 488 2.93e-36 137
MS.gene031625.t1 AT4G25360 27.632 380 222 13 20 355 150 520 8.98e-36 137
MS.gene031625.t1 AT4G25360 27.632 380 222 13 20 355 150 520 8.98e-36 137
MS.gene031625.t1 AT5G64020 29.444 360 205 15 42 359 56 408 3.85e-34 130
MS.gene031625.t1 AT2G37720 29.640 361 191 15 42 359 142 482 2.02e-32 127
MS.gene031625.t1 AT5G15900 25.862 406 242 14 1 359 16 409 3.17e-32 125
MS.gene031625.t1 AT5G15900 25.862 406 242 14 1 359 35 428 4.39e-32 125
MS.gene031625.t1 AT3G28150 27.577 388 244 13 5 361 31 412 2.44e-31 123
MS.gene031625.t1 AT1G01430 25.532 376 236 11 22 359 78 447 3.44e-30 120
MS.gene031625.t1 AT5G20680 27.855 359 198 15 43 359 118 457 6.90e-30 119
MS.gene031625.t1 AT5G20680 27.577 359 199 14 43 359 194 533 1.13e-29 119
MS.gene031625.t1 AT5G20680 27.577 359 199 14 43 359 212 551 1.15e-29 120
MS.gene031625.t1 AT5G20680 27.577 359 199 14 43 359 212 551 1.15e-29 120
MS.gene031625.t1 AT5G20680 27.577 359 199 14 43 359 212 551 1.15e-29 120
MS.gene031625.t1 AT5G20680 27.577 359 199 14 43 359 212 551 1.15e-29 120
MS.gene031625.t1 AT3G02440 24.928 349 223 10 43 359 135 476 6.99e-29 117
MS.gene031625.t1 AT3G02440 24.928 349 223 10 43 359 127 468 7.10e-29 117
MS.gene031625.t1 AT5G15890 26.724 348 204 12 43 355 187 518 6.05e-27 112
MS.gene031625.t1 AT5G64470 29.213 267 164 10 36 281 46 308 2.73e-24 101
MS.gene031625.t1 AT5G64470 29.213 267 164 10 36 281 46 308 4.12e-24 101
MS.gene031625.t1 AT4G01080 25.143 350 217 12 40 355 88 426 8.31e-24 102
MS.gene031625.t1 AT4G11090 24.866 374 223 13 30 359 66 425 1.46e-23 101
MS.gene031625.t1 AT4G23790 24.929 353 225 11 43 359 79 427 1.57e-21 95.5

Find 78 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAATGTGCTTCTAGAATT+TGG 0.184720 4.3:-29429572 None:intergenic
CAAACCAAAGTCTTCTTCTT+AGG 0.227258 4.3:+29431908 MS.gene031625:CDS
ATGGCATCCACCTATATTTC+TGG 0.239401 4.3:-29429336 None:intergenic
TGTAGCCACTGGTGTCTTCC+TGG 0.277598 4.3:+29432283 MS.gene031625:CDS
AGTGAGAAAGTGCCATATTT+TGG 0.279871 4.3:+29432103 MS.gene031625:CDS
CTTGGCATGTTACAAGAAAA+TGG 0.318130 4.3:-29429355 None:intergenic
AGAAGCTGATTCCAAGTATC+AGG 0.322948 4.3:-29432310 None:intergenic
GGGTATTTCAAACCAAAATA+TGG 0.332479 4.3:-29432115 None:intergenic
TGACAGATGGGATTATATTC+AGG 0.367092 4.3:+29431787 MS.gene031625:intron
TTCCTGGACTTCCTGATACT+TGG 0.369841 4.3:+29432299 MS.gene031625:CDS
TCACTCATGCATGACTTTGT+TGG 0.371357 4.3:-29432085 None:intergenic
ATAAGCTTTACCAGAAATAT+AGG 0.384304 4.3:+29429326 MS.gene031625:CDS
GTCACATCTAAGAAATAAAC+TGG 0.411760 4.3:-29432196 None:intergenic
AAGAAGACTTTGGTTTGTGC+TGG 0.422122 4.3:-29431902 None:intergenic
GGGAAGAAGATTATGTTTGT+TGG 0.424391 4.3:+29429491 MS.gene031625:CDS
CAAGCCATTGGTGTCCCTCC+AGG 0.427641 4.3:-29432136 None:intergenic
TTTGAAGTCAGTAACATTGC+AGG 0.428295 4.3:+29429187 MS.gene031625:CDS
AACTTTCTGAAGGGTAATAG+AGG 0.429506 4.3:+29429470 MS.gene031625:CDS
TCTATACCGCACAGCATACT+TGG 0.433438 4.3:+29431541 MS.gene031625:CDS
TGAAGGTTATAGTGGTGTTA+TGG 0.434673 4.3:+29432252 MS.gene031625:CDS
GTAACTTGTTCCGTGGCAAA+TGG 0.439848 4.3:+29429215 MS.gene031625:CDS
TATCTCTTCAGTCACATTTG+AGG 0.440220 4.3:+29429607 MS.gene031625:CDS
GTCCTTCTTTATCATGATCA+AGG 0.442671 4.3:-29431568 None:intergenic
TCTACAAAGGACTACAAACT+TGG 0.453097 4.3:+29431852 MS.gene031625:CDS
TACAAGCTAACGAATTGAAC+TGG 0.455479 4.3:-29429532 None:intergenic
AAATTGTTTCGCAGGTTCAA+TGG 0.460433 4.3:+29429443 MS.gene031625:intron
AATTCCTAAGAAGAAGACTT+TGG 0.470232 4.3:-29431912 None:intergenic
AATGTTACTGACTTCAAAGT+TGG 0.485805 4.3:-29429182 None:intergenic
CAATCAAGAGTGGTGAAGCT+TGG 0.495413 4.3:+29431609 MS.gene031625:CDS
AGGGACACCAATGGCTTGGA+TGG 0.502776 4.3:+29432141 MS.gene031625:CDS
TCTTCAACACGTGGCATTGG+TGG 0.505890 4.3:+29431651 MS.gene031625:CDS
TAACTTGTTCCGTGGCAAAT+GGG 0.506637 4.3:+29429216 MS.gene031625:CDS
GCACATCCTGAAGGTTATAG+TGG 0.507023 4.3:+29432244 MS.gene031625:CDS
CAATGGTTTGAACTTTCTGA+AGG 0.510925 4.3:+29429460 MS.gene031625:CDS
ATAACACCACTATAACCTTC+AGG 0.516042 4.3:-29432250 None:intergenic
CGGTTGTGAGCTTCCAGTGT+GGG 0.524560 4.3:-29431675 None:intergenic
TTGGTTTGAAATACCCTGGA+GGG 0.527368 4.3:+29432122 MS.gene031625:CDS
TGGTGAAGCTTGGAGAGGAA+TGG 0.527447 4.3:+29431619 MS.gene031625:CDS
CTGGAGGGACACCAATGGCT+TGG 0.529625 4.3:+29432137 MS.gene031625:CDS
TTCACCACCATCCAAGCCAT+TGG 0.532920 4.3:-29432148 None:intergenic
TCTATGAAAGGACAAGTTGA+AGG 0.533615 4.3:-29429268 None:intergenic
ATAACTTACTGGTAATGTAC+AGG 0.538647 4.3:-29431938 None:intergenic
TGATCTTCAACACGTGGCAT+TGG 0.541024 4.3:+29431648 MS.gene031625:CDS
TCATGCATGACTTTGTTGGT+TGG 0.542003 4.3:-29432081 None:intergenic
CAAGTTGAAGGGTTATAGAG+AGG 0.546976 4.3:-29429256 None:intergenic
TTCCAAGTATCAGGAAGTCC+AGG 0.548161 4.3:-29432301 None:intergenic
TAGAATTTGGTACAGCAGCA+TGG 0.554694 4.3:-29429559 None:intergenic
TTCCCACCAAACAAAATCAG+AGG 0.559578 4.3:+29429135 MS.gene031625:CDS
GACCTTGATCATGATAAAGA+AGG 0.566545 4.3:+29431566 MS.gene031625:CDS
GACTACAAACTTGGGCTAGG+TGG 0.570817 4.3:+29431861 MS.gene031625:CDS
ACTACAAACTTGGGCTAGGT+GGG 0.578379 4.3:+29431862 MS.gene031625:CDS
AAGCCATTGGTGTCCCTCCA+GGG 0.581911 4.3:-29432135 None:intergenic
CTACAAAGGACTACAAACTT+GGG 0.582833 4.3:+29431853 MS.gene031625:CDS
TGGCATTGGTGGACCCACAC+TGG 0.584418 4.3:+29431662 MS.gene031625:CDS
ATACCGCACAGCATACTTGG+TGG 0.586879 4.3:+29431544 MS.gene031625:CDS
TTGAACTGTGGATCTATGAA+AGG 0.589879 4.3:-29429280 None:intergenic
GGCTTGGATGGTGGTGAACA+AGG 0.591402 4.3:+29432153 MS.gene031625:CDS
AATGGTTTGAACTTTCTGAA+GGG 0.597673 4.3:+29429461 MS.gene031625:CDS
AGAAAAGATGCACATCCTGA+AGG 0.597997 4.3:+29432235 MS.gene031625:CDS
AAGGACTACAAACTTGGGCT+AGG 0.603384 4.3:+29431858 MS.gene031625:CDS
GACACCAATGGCTTGGATGG+TGG 0.610440 4.3:+29432144 MS.gene031625:CDS
CAGTTCAATTGTCAGAAACA+TGG 0.617132 4.3:+29429295 MS.gene031625:CDS
ACTTTCTGAAGGGTAATAGA+GGG 0.617389 4.3:+29429471 MS.gene031625:CDS
TGGCAACTGATTGTAGCCAC+TGG 0.618075 4.3:+29432272 MS.gene031625:CDS
CTATGAAAGGACAAGTTGAA+GGG 0.623253 4.3:-29429267 None:intergenic
TGGTGAACAAGGTGTTGAGA+AGG 0.632793 4.3:+29432164 MS.gene031625:CDS
ATACCCTGGAGGGACACCAA+TGG 0.646199 4.3:+29432132 MS.gene031625:CDS
AGCTTTACCAGAAATATAGG+TGG 0.648575 4.3:+29429329 MS.gene031625:CDS
ACGGTTGTGAGCTTCCAGTG+TGG 0.657143 4.3:-29431676 None:intergenic
AAGCTTGACTCAATCAAGAG+TGG 0.662012 4.3:+29431599 MS.gene031625:CDS
CGTCGTAGACCCATTTGCCA+CGG 0.673142 4.3:-29429225 None:intergenic
AAGAGTGGTGAAGCTTGGAG+AGG 0.679537 4.3:+29431614 MS.gene031625:CDS
GGAACATGTAACTTGTTCCG+TGG 0.681608 4.3:+29429208 MS.gene031625:CDS
TGTTTCTGACAATTGAACTG+TGG 0.711026 4.3:-29429292 None:intergenic
TTTGGTTTGAAATACCCTGG+AGG 0.715369 4.3:+29432121 MS.gene031625:CDS
GAAGTCCAGGAAGACACCAG+TGG 0.726350 4.3:-29432288 None:intergenic
ATGTGTTGATCTTCAACACG+TGG 0.756788 4.3:+29431642 MS.gene031625:CDS
GGTCCACCAAGTATGCTGTG+CGG 0.774384 4.3:-29431547 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAATATTAGGATTTTT+TGG - chr4.3:29431078-29431097 None:intergenic 10.0%
!!! TTTTTTATTTTTATTTTGAG+AGG - chr4.3:29430807-29430826 None:intergenic 10.0%
!! ATAATTCAATGTAATTACAA+GGG - chr4.3:29429745-29429764 None:intergenic 15.0%
!! ATTAGATATTAATAACTTAC+TGG - chr4.3:29431952-29431971 None:intergenic 15.0%
!!! ATTTTATTTATGATGAAAAG+TGG + chr4.3:29430384-29430403 MS.gene031625:intron 15.0%
!!! TAAATGTTATTGTTGTTTTT+AGG + chr4.3:29431499-29431518 MS.gene031625:intron 15.0%
!!! TATTTTTTTTGTTAAACATC+CGG + chr4.3:29430908-29430927 MS.gene031625:intron 15.0%
!!! TTAAGCTCTAAAAATATATA+TGG + chr4.3:29432030-29432049 MS.gene031625:intron 15.0%
!! AACATTGAACATTAAGATAA+CGG + chr4.3:29431388-29431407 MS.gene031625:intron 20.0%
!! ACCAATTAAAAAAACACTAT+TGG - chr4.3:29429872-29429891 None:intergenic 20.0%
!! ATCAACAAAGAGAAAATAAA+CGG + chr4.3:29430251-29430270 MS.gene031625:intron 20.0%
!! CATAATTCAATGTAATTACA+AGG - chr4.3:29429746-29429765 None:intergenic 20.0%
!! CTAAAAATATATATGGTGTA+TGG + chr4.3:29432037-29432056 MS.gene031625:intron 20.0%
!! TAAAATACGTAATGAATTTG+TGG - chr4.3:29430370-29430389 None:intergenic 20.0%
!! TTAAATACCAACAAAACTTT+AGG - chr4.3:29430956-29430975 None:intergenic 20.0%
!! TTAGCATCATTAATTAATCA+TGG - chr4.3:29431743-29431762 None:intergenic 20.0%
!!! ACCAATAGTGTTTTTTTAAT+TGG + chr4.3:29429868-29429887 MS.gene031625:intron 20.0%
!!! CAAAGTGTTAATATAAAAAC+CGG - chr4.3:29430930-29430949 None:intergenic 20.0%
!!! TTTATTTGACAACGTTTTAT+AGG - chr4.3:29430868-29430887 None:intergenic 20.0%
!!! TTTTGAAAATGAAGTAACAA+GGG - chr4.3:29430710-29430729 None:intergenic 20.0%
!!! TTTTTTGGAATCCTAAAATT+CGG - chr4.3:29431063-29431082 None:intergenic 20.0%
! AAAACAATGAAAGTGAATCA+CGG - chr4.3:29429833-29429852 None:intergenic 25.0%
! AAGCAATTAATCAGATTTAC+TGG - chr4.3:29431260-29431279 None:intergenic 25.0%
! AATGTGACTGGTATAATTAT+TGG + chr4.3:29431325-29431344 MS.gene031625:intron 25.0%
! AATTAATCAGATTTACTGGT+AGG - chr4.3:29431256-29431275 None:intergenic 25.0%
! ATAAGCTTTACCAGAAATAT+AGG + chr4.3:29429326-29429345 MS.gene031625:CDS 25.0%
! ATATATGGTGTATGGTATTA+GGG + chr4.3:29432045-29432064 MS.gene031625:intron 25.0%
! ATTTATGATGAAAAGTGGAA+TGG + chr4.3:29430389-29430408 MS.gene031625:intron 25.0%
! CAATAAAGAATTGATGTGAT+TGG + chr4.3:29430080-29430099 MS.gene031625:intron 25.0%
! GTTTGAGAAACAATAAAGAA+TGG - chr4.3:29430314-29430333 None:intergenic 25.0%
! TAAAAAATTGCCACAAAAGT+GGG - chr4.3:29430186-29430205 None:intergenic 25.0%
! TATATATGGTGTATGGTATT+AGG + chr4.3:29432044-29432063 MS.gene031625:intron 25.0%
! TTGAAAAATTCATGACAGAT+GGG + chr4.3:29431775-29431794 MS.gene031625:intron 25.0%
! TTTATGATGAAAAGTGGAAT+GGG + chr4.3:29430390-29430409 MS.gene031625:intron 25.0%
!! GTTTTATAGGATAAAGTTCA+AGG - chr4.3:29430855-29430874 None:intergenic 25.0%
!! TAAAATCCTCTGATTTTGTT+TGG - chr4.3:29429144-29429163 None:intergenic 25.0%
!!! ATTGTTGTTTTTAGGAGTAT+GGG + chr4.3:29431507-29431526 MS.gene031625:intron 25.0%
!!! CTACTCTTTATTTTTTTGAG+AGG + chr4.3:29430282-29430301 MS.gene031625:intron 25.0%
!!! CTTTTTTATTAGAACAGACA+TGG + chr4.3:29430333-29430352 MS.gene031625:intron 25.0%
!!! GTTTTGAAAATGAAGTAACA+AGG - chr4.3:29430711-29430730 None:intergenic 25.0%
!!! TATTGTTGTTTTTAGGAGTA+TGG + chr4.3:29431506-29431525 MS.gene031625:intron 25.0%
!!! TGTTCTTTTGTTGGTTTTTA+TGG + chr4.3:29429401-29429420 MS.gene031625:intron 25.0%
AAAGAATTGATGTGATTGGT+TGG + chr4.3:29430084-29430103 MS.gene031625:intron 30.0%
AATGGTTTGAACTTTCTGAA+GGG + chr4.3:29429461-29429480 MS.gene031625:CDS 30.0%
AATTCCTAAGAAGAAGACTT+TGG - chr4.3:29431915-29431934 None:intergenic 30.0%
ACCAACAAAAGAACACAAAA+TGG - chr4.3:29429396-29429415 None:intergenic 30.0%
ACTAACAAAGTCTACATTCA+GGG + chr4.3:29430585-29430604 MS.gene031625:intron 30.0%
ATGGGACCATGAAAAAAAAA+GGG - chr4.3:29431170-29431189 None:intergenic 30.0%
CTAGACAAGAAAATGAACTT+TGG + chr4.3:29430780-29430799 MS.gene031625:intron 30.0%
CTTGAAAAATTCATGACAGA+TGG + chr4.3:29431774-29431793 MS.gene031625:intron 30.0%
GGGTATTTCAAACCAAAATA+TGG - chr4.3:29432118-29432137 None:intergenic 30.0%
GTAAAAAATTGCCACAAAAG+TGG - chr4.3:29430187-29430206 None:intergenic 30.0%
GTCACATCTAAGAAATAAAC+TGG - chr4.3:29432199-29432218 None:intergenic 30.0%
TATATGGTGTATGGTATTAG+GGG + chr4.3:29432046-29432065 MS.gene031625:intron 30.0%
TGGTAACAAAAATTGTACCT+TGG - chr4.3:29429376-29429395 None:intergenic 30.0%
TTATGATGAAAAGTGGAATG+GGG + chr4.3:29430391-29430410 MS.gene031625:intron 30.0%
TTCAATGTAATTACAAGGGT+CGG - chr4.3:29429741-29429760 None:intergenic 30.0%
TTGCAATTTATCTGCATTCA+GGG + chr4.3:29430614-29430633 MS.gene031625:intron 30.0%
TTGCAATTTCTCTTCATTCA+GGG - chr4.3:29430464-29430483 None:intergenic 30.0%
TTTGCAATTTATCTGCATTC+AGG + chr4.3:29430613-29430632 MS.gene031625:intron 30.0%
TTTGCAATTTCTCTTCATTC+AGG - chr4.3:29430465-29430484 None:intergenic 30.0%
! ACCATTTTGTGTTCTTTTGT+TGG + chr4.3:29429392-29429411 MS.gene031625:intron 30.0%
! ATAACTTACTGGTAATGTAC+AGG - chr4.3:29431941-29431960 None:intergenic 30.0%
! TGAGTAATCAACCGAATTTT+AGG + chr4.3:29431049-29431068 MS.gene031625:intron 30.0%
! TGCTATTTATACAATGTGAC+TGG + chr4.3:29431313-29431332 MS.gene031625:intron 30.0%
! TTCATTACATTTGCTGAAAC+AGG - chr4.3:29431196-29431215 None:intergenic 30.0%
!! AATGTTACTGACTTCAAAGT+TGG - chr4.3:29429185-29429204 None:intergenic 30.0%
!! TTTGACACCTAAAGTTTTGT+TGG + chr4.3:29430946-29430965 MS.gene031625:intron 30.0%
!!! ACGAAACCCTTTTTTTTTCA+TGG + chr4.3:29431161-29431180 MS.gene031625:intron 30.0%
!!! TATTTTGGTTTGAAATACCC+TGG + chr4.3:29432118-29432137 MS.gene031625:CDS 30.0%
AAAGAAACAATTCCACTCTG+GGG - chr4.3:29430687-29430706 None:intergenic 35.0%
AAATTGTTTCGCAGGTTCAA+TGG + chr4.3:29429443-29429462 MS.gene031625:intron 35.0%
AATGTGAGAGAATGATCCTA+CGG - chr4.3:29431698-29431717 None:intergenic 35.0%
ACTAACGAAGTTTGCATTCA+GGG + chr4.3:29430553-29430572 MS.gene031625:intron 35.0%
AGCTTTACCAGAAATATAGG+TGG + chr4.3:29429329-29429348 MS.gene031625:CDS 35.0%
ATAACACCACTATAACCTTC+AGG - chr4.3:29432253-29432272 None:intergenic 35.0%
ATATGGTGTATGGTATTAGG+GGG + chr4.3:29432047-29432066 MS.gene031625:intron 35.0%
ATGAAAGTGAATCACGGTTA+CGG - chr4.3:29429827-29429846 None:intergenic 35.0%
CAAACCAAAGTCTTCTTCTT+AGG + chr4.3:29431908-29431927 MS.gene031625:CDS 35.0%
CAATGGTTTGAACTTTCTGA+AGG + chr4.3:29429460-29429479 MS.gene031625:CDS 35.0%
CAGTTCAATTGTCAGAAACA+TGG + chr4.3:29429295-29429314 MS.gene031625:CDS 35.0%
CATGGGACCATGAAAAAAAA+AGG - chr4.3:29431171-29431190 None:intergenic 35.0%
CATTATGTGCATGTTTGAGT+TGG - chr4.3:29430209-29430228 None:intergenic 35.0%
CTACAAAGGACTACAAACTT+GGG + chr4.3:29431853-29431872 MS.gene031625:CDS 35.0%
CTTGGCATGTTACAAGAAAA+TGG - chr4.3:29429358-29429377 None:intergenic 35.0%
GAAAGAAACAATTCCACTCT+GGG - chr4.3:29430688-29430707 None:intergenic 35.0%
GACCTTGATCATGATAAAGA+AGG + chr4.3:29431566-29431585 MS.gene031625:CDS 35.0%
GACTAACAAAGTCTACATTC+AGG + chr4.3:29430584-29430603 MS.gene031625:intron 35.0%
GGGAAGAAGATTATGTTTGT+TGG + chr4.3:29429491-29429510 MS.gene031625:CDS 35.0%
GTCCTTCTTTATCATGATCA+AGG - chr4.3:29431571-29431590 None:intergenic 35.0%
GTTGTGATAAATTGTTTCGC+AGG + chr4.3:29429435-29429454 MS.gene031625:intron 35.0%
TAATCAGATTTACTGGTAGG+TGG - chr4.3:29431253-29431272 None:intergenic 35.0%
TACAAGCTAACGAATTGAAC+TGG - chr4.3:29429535-29429554 None:intergenic 35.0%
TATCTCTTCAGTCACATTTG+AGG + chr4.3:29429607-29429626 MS.gene031625:CDS 35.0%
TCTACAAAGGACTACAAACT+TGG + chr4.3:29431852-29431871 MS.gene031625:CDS 35.0%
TGACAGATGGGATTATATTC+AGG + chr4.3:29431787-29431806 MS.gene031625:intron 35.0%
TGGTCATAAGTGTATCAAAC+TGG + chr4.3:29431345-29431364 MS.gene031625:intron 35.0%
TTCGTGCTTCATTACACAAA+AGG - chr4.3:29429662-29429681 None:intergenic 35.0%
TTGAACTGTGGATCTATGAA+AGG - chr4.3:29429283-29429302 None:intergenic 35.0%
TTTGAGTATTACAAGCCTTG+AGG + chr4.3:29430010-29430029 MS.gene031625:intron 35.0%
! AACTTTCTGAAGGGTAATAG+AGG + chr4.3:29429470-29429489 MS.gene031625:CDS 35.0%
! ACTTTCTGAAGGGTAATAGA+GGG + chr4.3:29429471-29429490 MS.gene031625:CDS 35.0%
! CACAAAAGGCATAAAAGTGA+AGG - chr4.3:29429648-29429667 None:intergenic 35.0%
! CATTTTCTTGTAACATGCCA+AGG + chr4.3:29429356-29429375 MS.gene031625:CDS 35.0%
! CTATGAAAGGACAAGTTGAA+GGG - chr4.3:29429270-29429289 None:intergenic 35.0%
! TCTATGAAAGGACAAGTTGA+AGG - chr4.3:29429271-29429290 None:intergenic 35.0%
! TGAAGGTTATAGTGGTGTTA+TGG + chr4.3:29432252-29432271 MS.gene031625:CDS 35.0%
! TGTTTCTGACAATTGAACTG+TGG - chr4.3:29429295-29429314 None:intergenic 35.0%
! TTTGAAGTCAGTAACATTGC+AGG + chr4.3:29429187-29429206 MS.gene031625:CDS 35.0%
!! AATCCTCTGATTTTGTTTGG+TGG - chr4.3:29429141-29429160 None:intergenic 35.0%
!! ATCCTCTGATTTTGTTTGGT+GGG - chr4.3:29429140-29429159 None:intergenic 35.0%
!! CGTTTTGTTGCATTCTACAA+AGG + chr4.3:29431839-29431858 MS.gene031625:CDS 35.0%
!! CTGAATGTGCTTCTAGAATT+TGG - chr4.3:29429575-29429594 None:intergenic 35.0%
!!! AGTGAGAAAGTGCCATATTT+TGG + chr4.3:29432103-29432122 MS.gene031625:CDS 35.0%
!!! CTTGTCTAGTTCTTTTGCAT+AGG - chr4.3:29430769-29430788 None:intergenic 35.0%
!!! CTTTTTTTTTCATGGTCCCA+TGG + chr4.3:29431169-29431188 MS.gene031625:intron 35.0%
AAGCTTGACTCAATCAAGAG+TGG + chr4.3:29431599-29431618 MS.gene031625:CDS 40.0%
ACCATCATGTTCTAACCTCA+AGG - chr4.3:29430028-29430047 None:intergenic 40.0%
ACTAACGGAGTCTACATTCA+GGG + chr4.3:29430521-29430540 MS.gene031625:intron 40.0%
ACTTTAGTCCCTGAATCAGA+AGG - chr4.3:29430656-29430675 None:intergenic 40.0%
AGAAAAGATGCACATCCTGA+AGG + chr4.3:29432235-29432254 MS.gene031625:CDS 40.0%
AGAAAATGTCCCCAACACAA+AGG - chr4.3:29430117-29430136 None:intergenic 40.0%
ATGGCATCCACCTATATTTC+TGG - chr4.3:29429339-29429358 None:intergenic 40.0%
CTTTAGTCCCTGAATCAGAA+GGG - chr4.3:29430655-29430674 None:intergenic 40.0%
GACAAATCCCTTCTGATTCA+GGG + chr4.3:29430645-29430664 MS.gene031625:intron 40.0%
GACTAACGAAGTTTGCATTC+AGG + chr4.3:29430552-29430571 MS.gene031625:intron 40.0%
GGAAAGAAACAATTCCACTC+TGG - chr4.3:29430689-29430708 None:intergenic 40.0%
TAGAATTTGGTACAGCAGCA+TGG - chr4.3:29429562-29429581 None:intergenic 40.0%
TCACTCATGCATGACTTTGT+TGG - chr4.3:29432088-29432107 None:intergenic 40.0%
TCAGGGACTAAAGTGACTAA+CGG + chr4.3:29430506-29430525 MS.gene031625:intron 40.0%
TCATGCATGACTTTGTTGGT+TGG - chr4.3:29432084-29432103 None:intergenic 40.0%
TCCTTGGATTATGTGTGTCT+CGG + chr4.3:29429685-29429704 MS.gene031625:intron 40.0%
TGACAAATCCCTTCTGATTC+AGG + chr4.3:29430644-29430663 MS.gene031625:intron 40.0%
TTCCCACCAAACAAAATCAG+AGG + chr4.3:29429135-29429154 MS.gene031625:CDS 40.0%
! AGAAGCTGATTCCAAGTATC+AGG - chr4.3:29432313-29432332 None:intergenic 40.0%
! AGTCACTTTAGTCCCTGAAT+GGG + chr4.3:29430418-29430437 MS.gene031625:intron 40.0%
! ATGTGTTGATCTTCAACACG+TGG + chr4.3:29431642-29431661 MS.gene031625:CDS 40.0%
! ATTTGCTGAAACAGGACCAT+GGG - chr4.3:29431188-29431207 None:intergenic 40.0%
! CAAGTTGAAGGGTTATAGAG+AGG - chr4.3:29429259-29429278 None:intergenic 40.0%
! GAATTGATGTGATTGGTTGG+AGG + chr4.3:29430087-29430106 MS.gene031625:intron 40.0%
! TAGTCACTTTAGTCCCTGAA+TGG + chr4.3:29430417-29430436 MS.gene031625:intron 40.0%
! TCTTCAGTCACATTTGAGGT+TGG + chr4.3:29429611-29429630 MS.gene031625:intron 40.0%
! TTGGTTTGAAATACCCTGGA+GGG + chr4.3:29432122-29432141 MS.gene031625:CDS 40.0%
!! AAGAAGACTTTGGTTTGTGC+TGG - chr4.3:29431905-29431924 None:intergenic 40.0%
!! GATACTTAGACCCACTTTTG+TGG + chr4.3:29430173-29430192 MS.gene031625:intron 40.0%
!! TAACTTGTTCCGTGGCAAAT+GGG + chr4.3:29429216-29429235 MS.gene031625:CDS 40.0%
!! TTTGGTTTGAAATACCCTGG+AGG + chr4.3:29432121-29432140 MS.gene031625:CDS 40.0%
AAGGACTACAAACTTGGGCT+AGG + chr4.3:29431858-29431877 MS.gene031625:CDS 45.0%
AAGTGACTAATTCCCCAGAG+TGG + chr4.3:29430672-29430691 MS.gene031625:intron 45.0%
AATGAAGCACGAACACTCCT+TGG + chr4.3:29429669-29429688 MS.gene031625:intron 45.0%
ACCGAGACACACATAATCCA+AGG - chr4.3:29429689-29429708 None:intergenic 45.0%
ATGGTATTAGGGGGAGAGAT+TGG + chr4.3:29432056-29432075 MS.gene031625:intron 45.0%
ATTGCAAAACAGTCCCTGCA+TGG + chr4.3:29430477-29430496 MS.gene031625:intron 45.0%
CAATCAAGAGTGGTGAAGCT+TGG + chr4.3:29431609-29431628 MS.gene031625:CDS 45.0%
CAGATTTACTGGTAGGTGGT+AGG - chr4.3:29431249-29431268 None:intergenic 45.0%
GACTAACGGAGTCTACATTC+AGG + chr4.3:29430520-29430539 MS.gene031625:intron 45.0%
GCACATCCTGAAGGTTATAG+TGG + chr4.3:29432244-29432263 MS.gene031625:CDS 45.0%
GCCTTGAGGTTAGAACATGA+TGG + chr4.3:29430024-29430043 MS.gene031625:intron 45.0%
TCCCTGAAATTGACCATGCA+GGG - chr4.3:29430493-29430512 None:intergenic 45.0%
TCCCTGCATGGTCAATTTCA+GGG + chr4.3:29430489-29430508 MS.gene031625:intron 45.0%
TCTATACCGCACAGCATACT+TGG + chr4.3:29431541-29431560 MS.gene031625:CDS 45.0%
TGATCTTCAACACGTGGCAT+TGG + chr4.3:29431648-29431667 MS.gene031625:CDS 45.0%
TGGAGGCTTTCCTTTGTGTT+GGG + chr4.3:29430104-29430123 MS.gene031625:intron 45.0%
TTCCAAGTATCAGGAAGTCC+AGG - chr4.3:29432304-29432323 None:intergenic 45.0%
TTCCTGGACTTCCTGATACT+TGG + chr4.3:29432299-29432318 MS.gene031625:CDS 45.0%
TTGGAGGCTTTCCTTTGTGT+TGG + chr4.3:29430103-29430122 MS.gene031625:intron 45.0%
! ACTACAAACTTGGGCTAGGT+GGG + chr4.3:29431862-29431881 MS.gene031625:CDS 45.0%
! CATTTGCTGAAACAGGACCA+TGG - chr4.3:29431189-29431208 None:intergenic 45.0%
! GATTATGTGTGTCTCGGTGT+CGG + chr4.3:29429691-29429710 MS.gene031625:intron 45.0%
!! GGAACATGTAACTTGTTCCG+TGG + chr4.3:29429208-29429227 MS.gene031625:CDS 45.0%
!! GTAACTTGTTCCGTGGCAAA+TGG + chr4.3:29429215-29429234 MS.gene031625:CDS 45.0%
!! TGGTGAACAAGGTGTTGAGA+AGG + chr4.3:29432164-29432183 MS.gene031625:CDS 45.0%
!! TAATGAATTAAAAAAATATT+AGG - chr4.3:29431087-29431106 None:intergenic 5.0%
AAGAGTGGTGAAGCTTGGAG+AGG + chr4.3:29431614-29431633 MS.gene031625:CDS 50.0%
ATACCGCACAGCATACTTGG+TGG + chr4.3:29431544-29431563 MS.gene031625:CDS 50.0%
CTTTAGTCCCTGAATGGGAG+AGG + chr4.3:29430423-29430442 MS.gene031625:intron 50.0%
GACGAATCCTCTCCCATTCA+GGG - chr4.3:29430433-29430452 None:intergenic 50.0%
GGAGGCTTTCCTTTGTGTTG+GGG + chr4.3:29430105-29430124 MS.gene031625:intron 50.0%
GTCCCTGAAATTGACCATGC+AGG - chr4.3:29430494-29430513 None:intergenic 50.0%
GTCCCTGCATGGTCAATTTC+AGG + chr4.3:29430488-29430507 MS.gene031625:intron 50.0%
TGAATCACGGTTACGGACAC+TGG - chr4.3:29429820-29429839 None:intergenic 50.0%
TGACGAATCCTCTCCCATTC+AGG - chr4.3:29430434-29430453 None:intergenic 50.0%
TGGCAACTGATTGTAGCCAC+TGG + chr4.3:29432272-29432291 MS.gene031625:CDS 50.0%
TGGTGAAGCTTGGAGAGGAA+TGG + chr4.3:29431619-29431638 MS.gene031625:CDS 50.0%
TTCACCACCATCCAAGCCAT+TGG - chr4.3:29432151-29432170 None:intergenic 50.0%
! GACTACAAACTTGGGCTAGG+TGG + chr4.3:29431861-29431880 MS.gene031625:CDS 50.0%
! TCTTCAACACGTGGCATTGG+TGG + chr4.3:29431651-29431670 MS.gene031625:CDS 50.0%
!! TACAAGGGTCGGTGTCGTAT+CGG - chr4.3:29429730-29429749 None:intergenic 50.0%
ACGGTTGTGAGCTTCCAGTG+TGG - chr4.3:29431679-29431698 None:intergenic 55.0%
ATACCCTGGAGGGACACCAA+TGG + chr4.3:29432132-29432151 MS.gene031625:CDS 55.0%
CACTGGAAGCTCACAACCGT+AGG + chr4.3:29431679-29431698 MS.gene031625:intron 55.0%
CGGTTGTGAGCTTCCAGTGT+GGG - chr4.3:29431678-29431697 None:intergenic 55.0%
CGTCGTAGACCCATTTGCCA+CGG - chr4.3:29429228-29429247 None:intergenic 55.0%
GAAGTCCAGGAAGACACCAG+TGG - chr4.3:29432291-29432310 None:intergenic 55.0%
GGTCCACCAAGTATGCTGTG+CGG - chr4.3:29431550-29431569 None:intergenic 55.0%
TGTAGCCACTGGTGTCTTCC+TGG + chr4.3:29432283-29432302 MS.gene031625:CDS 55.0%
! AAGCCATTGGTGTCCCTCCA+GGG - chr4.3:29432138-29432157 None:intergenic 55.0%
! AGGGACACCAATGGCTTGGA+TGG + chr4.3:29432141-29432160 MS.gene031625:CDS 55.0%
! GACACCAATGGCTTGGATGG+TGG + chr4.3:29432144-29432163 MS.gene031625:CDS 55.0%
! GGCTTGGATGGTGGTGAACA+AGG + chr4.3:29432153-29432172 MS.gene031625:CDS 55.0%
CTGGAGGGACACCAATGGCT+TGG + chr4.3:29432137-29432156 MS.gene031625:CDS 60.0%
! CAAGCCATTGGTGTCCCTCC+AGG - chr4.3:29432139-29432158 None:intergenic 60.0%
!! TGGCATTGGTGGACCCACAC+TGG + chr4.3:29431662-29431681 MS.gene031625:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 29429088 29432354 29429088 ID=MS.gene031625
chr4.3 mRNA 29429088 29432354 29429088 ID=MS.gene031625.t1;Parent=MS.gene031625
chr4.3 exon 29429088 29429377 29429088 ID=MS.gene031625.t1.exon1;Parent=MS.gene031625.t1
chr4.3 CDS 29429088 29429377 29429088 ID=cds.MS.gene031625.t1;Parent=MS.gene031625.t1
chr4.3 exon 29429457 29429628 29429457 ID=MS.gene031625.t1.exon2;Parent=MS.gene031625.t1
chr4.3 CDS 29429457 29429628 29429457 ID=cds.MS.gene031625.t1;Parent=MS.gene031625.t1
chr4.3 exon 29431521 29431696 29431521 ID=MS.gene031625.t1.exon3;Parent=MS.gene031625.t1
chr4.3 CDS 29431521 29431696 29431521 ID=cds.MS.gene031625.t1;Parent=MS.gene031625.t1
chr4.3 exon 29431793 29431951 29431793 ID=MS.gene031625.t1.exon4;Parent=MS.gene031625.t1
chr4.3 CDS 29431793 29431951 29431793 ID=cds.MS.gene031625.t1;Parent=MS.gene031625.t1
chr4.3 exon 29432066 29432354 29432066 ID=MS.gene031625.t1.exon5;Parent=MS.gene031625.t1
chr4.3 CDS 29432066 29432354 29432066 ID=cds.MS.gene031625.t1;Parent=MS.gene031625.t1
Gene Sequence

>MS.gene031625

ATGGTTTCAGTGCTTGTAAATGCTCTGCTTCTAACTCTGTTTCTGTTTTCCCACCAAACAAAATCAGAGGATTTTAACTCTTTCAGCAATGAGACCAACTTTGAAGTCAGTAACATTGCAGGAACATGTAACTTGTTCCGTGGCAAATGGGTCTACGACGCTTCATATCCTCTCTATAACCCTTCAACTTGTCCTTTCATAGATCCACAGTTCAATTGTCAGAAACATGGTCGCAAAGATAAGCTTTACCAGAAATATAGGTGGATGCCATTTTCTTGTAACATGCCAAGGTACAATTTTTGTTACCATTTTGTGTTCTTTTGTTGGTTTTTATGGTTGTAGCTATAGTTGTGATAAATTGTTTCGCAGGTTCAATGGTTTGAACTTTCTGAAGGGTAATAGAGGGAAGAAGATTATGTTTGTTGGTGACTCTTTGAGCTTAAACCAGTTCAATTCGTTAGCTTGTATGATCCATGCTGCTGTACCAAATTCTAGAAGCACATTCAGACAAAGAGATGCTATCTCTTCAGTCACATTTGAGGTTGGTTCTTTTCATTCCTTCACTTTTATGCCTTTTGTGTAATGAAGCACGAACACTCCTTGGATTATGTGTGTCTCGGTGTCGGACACGTGTCGCGTCCGATACGACACCGACCCTTGTAATTACATTGAATTATGTGATTTTTTCTAATTATTAGTTGTGTCAGTGTGTCAGTGTCTGTGTCATGTCCAGTGTCCGTAACCGTGATTCACTTTCATTGTTTTAAAATTTAAATTTAAACCAATAGTGTTTTTTTAATTGGTTTTTTGTTTGTTTTAATGATGAAAGCTAACTAAGTCATGTCATTTTCTGTTTTGCTTTTTGAAACTTTTGTTGTGTTATTACAAAATTGAACTGAAATTTTGTGACACTTCTGATCTATTTGAGTATTACAAGCCTTGAGGTTAGAACATGATGGTGTGCATATTTGTGTGTTAAAGTGAAGTTTGAGCAATAAAGAATTGATGTGATTGGTTGGAGGCTTTCCTTTGTGTTGGGGACATTTTCTAATGATAATTTAAGAGATAATAAATGTTTGTCTTCAGATACTTAGACCCACTTTTGTGGCAATTTTTTACCAACTCAAACATGCACATAATGTAATTTTTTTCATCTAGTCTAGATCAACAAAGAGAAAATAAACGGTACTAGTACTACTCTTTATTTTTTTGAGAGGATAGTACCATTCTTTATTGTTTCTCAAACTTTTTTATTAGAACAGACATGGAGTTTCAAATGCCACAAATTCATTACGTATTTTATTTATGATGAAAAGTGGAATGGGGAATTAGTCACTTTAGTCCCTGAATGGGAGAGGATTCGTCACTTTAGTCCCTGAATGAAGAGAAATTGCAAAACAGTCCCTGCATGGTCAATTTCAGGGACTAAAGTGACTAACGGAGTCTACATTCAGGGACTAAATTGACTAACGAAGTTTGCATTCAGGGACTAAATTGACTAACAAAGTCTACATTCAGGGACTATTTTGCAATTTATCTGCATTCAGGGACTAACGTGACAAATCCCTTCTGATTCAGGGACTAAAGTGACTAATTCCCCAGAGTGGAATTGTTTCTTTCCCTTGTTACTTCATTTTCAAAACTTGCTTAAAGCAAACTACAGTAATTTTCAGCAGTTCCTATGCAAAAGAACTAGACAAGAAAATGAACTTTGGTCCTCTCAAAATAAAAATAAAAAATGAACTTTGTTTTTGACATAATAAACCTTGAACTTTATCCTATAAAACGTTGTCAAATAAATTTATGTTTAGAGCAATGATTATTTTTTTTGTTAAACATCCGGTTTTTATATTAACACTTTGACACCTAAAGTTTTGTTGGTATTTAACATCTAATAATTTAATTCTATGCTATTTTTTAAATTATTATAAAGTGAACTTAAATTTTGAATTAATTTTATATGAGTAATCAACCGAATTTTAGGATTCCAAAAAATCCTAATATTTTTTTAATTCATTACATTTGTTGTAACTAAATTATGATATAGACAACGACTGCAACAAACATTGCTTTACGAAACCCTTTTTTTTTCATGGTCCCATGGTCCTGTTTCAGCAAATGTAATGAATGATAATAATTTGCTATTTATCATTTTTTTCCTACCACCTACCAGTAAATCTGATTAATTGCTTCATAATGTTTGTTTATCATATAAGTTTTCATAATGCTATTTATACAATGTGACTGGTATAATTATTGGTCATAAGTGTATCAAACTGGAACTTCAATAGTGACATACTAACATTGAACATTAAGATAACGGTTATAGCTTGTGAAATAAGTAAATAACTAACTACATATAATTTATGAAAAGAACGTGAAAATGAACACAAGCAATCTATGTATATAACTAAATGTTATTGTTGTTTTTAGGAGTATGGGCTTGAGTTATTTCTATACCGCACAGCATACTTGGTGGACCTTGATCATGATAAAGAAGGACGAGTATTGAAGCTTGACTCAATCAAGAGTGGTGAAGCTTGGAGAGGAATGGATGTGTTGATCTTCAACACGTGGCATTGGTGGACCCACACTGGAAGCTCACAACCGTAGGATCATTCTCTCACATTCATATTATTCATATTTTTATTGCCATGATTAATTAATGATGCTAATGTTTTTGTGACTTGAAAAATTCATGACAGATGGGATTATATTCAGGAGAATAAAAAGTTGTATAAAGACATGAATCGTTTTGTTGCATTCTACAAAGGACTACAAACTTGGGCTAGGTGGGTTGAAATGAATGTTAATCCAGCACAAACCAAAGTCTTCTTCTTAGGAATTTCTCCTGTACATTACCAGTAAGTTATTAATATCTAATTAATTAAATGTATGCATTAATCAACACACATTGAACATTTTTGTACATTGTATTAAAATTAAGCTCTAAAAATATATATGGTGTATGGTATTAGGGGGAGAGATTGGAACCAACCAACAAAGTCATGCATGAGTGAGAAAGTGCCATATTTTGGTTTGAAATACCCTGGAGGGACACCAATGGCTTGGATGGTGGTGAACAAGGTGTTGAGAAGGATGAGTAAGCCAGTTTATTTCTTAGATGTGACAACTCTTTCACAATACAGAAAAGATGCACATCCTGAAGGTTATAGTGGTGTTATGGCAACTGATTGTAGCCACTGGTGTCTTCCTGGACTTCCTGATACTTGGAATCAGCTTCTCTATGCAGCACTTTCTCATTGA
Protein sequence

>MS.gene031625.t1

MVSVLVNALLLTLFLFSHQTKSEDFNSFSNETNFEVSNIAGTCNLFRGKWVYDASYPLYNPSTCPFIDPQFNCQKHGRKDKLYQKYRWMPFSCNMPRFNGLNFLKGNRGKKIMFVGDSLSLNQFNSLACMIHAAVPNSRSTFRQRDAISSVTFEEYGLELFLYRTAYLVDLDHDKEGRVLKLDSIKSGEAWRGMDVLIFNTWHWWTHTGSSQPWDYIQENKKLYKDMNRFVAFYKGLQTWARWVEMNVNPAQTKVFFLGISPVHYQGRDWNQPTKSCMSEKVPYFGLKYPGGTPMAWMVVNKVLRRMSKPVYFLDVTTLSQYRKDAHPEGYSGVMATDCSHWCLPGLPDTWNQLLYAALSH